名称: | pCMV-CNTNAP4(human)-Myc-6×His |
---|---|
别称: | NM_033401.5;CNTNAP4/CASPR4 |
基因长: | 3924bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCDNA4-Myc-HisB |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTCGACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAC
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGT
TAAGCTTGGT
ACCGAGCTCG
GATCCGCCAC
CATGGGATCT
GTCACGGGAG
CTGTCCTCAA
GACGCTACTT
CTGTTATCTA
CTCAAAATTG
GAACAGAGTC
GAAGCTGGGA
ATTCCTATGA
CTGTGATGAT
CCTCTTGTGT
CTGCCTTGCC
TCAGGCATCC
TTCAGCAGTT
CTTCCGAGCT
CTCCAGCAGT
CATGGTCCTG
GATTTGCAAG
GCTGAATAGA
AGAGATGGAG
CTGGTGGCTG
GTCTCCACTT
GTGTCTAACA
AATACCAGTG
GTTGCAGATT
GACCTTGGAG
AGAGAATGGA
GGTCACCGCT
GTGGCCACTC
AAGGGGGATA
TGGTAGCTCC
AACTGGGTGA
CCAGCTACCT
CCTGATGTTC
AGTGATAGTG
GCTGGAACTG
GAAACAATAT
CGCCAAGAGG
ACAGCATCTG
GGGTTTTTCA
GGAAATGCAA
ATGCAGACAG
TGTTGTGTAC
TATAGACTCC
AGCCTTCTAT
CAAAGCCAGA
TTTCTGCGCT
TCATCCCTTT
GGAATGGAAC
CCCAAGGGCA
GAATTGGAAT
GCGAATCGAA
GTGTTCGGAT
GTGCATACAG
ATCAGAAGTG
GTTGATCTTG
ATGGAAAAAG
TTCCCTTCTC
TACAGATTTG
ATCAAAAATC
CCTGAGCCCA
ATAAAAGACA
TTATTTCTTT
GAAATTCAAA
ACCATGCAGA
GTGATGGGAT
TCTACTCCAC
AGGGAAGGGC
CAAATGGAGA
TCACATCACA
CTGCAATTAA
GAAGAGCAAG
ACTCTTTTTA
CTTATTAATT
CAGGTGAAGC
TAAACTGCCT
TCCACTTCCA
CCCTGGTCAA
TCTCACCCTG
GGCAGCCTGC
TAGATGATCA
GCATTGGCAT
TCAGTGCTCA
TCCAGCGTTT
GGGCAAACAA
GTCAACTTCA
CAGTGGACGA
ACACAGGCAT
CATTTCCATG
CACGGGGAGA
ATTCAATCTC
ATGAATCTTG
ATTATGAGAT
CAGCTTTGGA
GGGATTCCAG
CACCTGGAAA
ATCAGTGTCA
TTCCCACATA
GAAATTTTCA
TGGATGTTTA
GAAAATCTCT
ATTATAATGG
AGTGGATATC
ATTGATTTGG
CCAAGCAGCA
AAAACCACAG
ATCATTGCTA
TGGGAAATGT
GTCATTTTCT
TGTTCACAAC
CACAATCTAT
GCCCGTGACT
TTTCTGAGCT
CCAGGAGTTA
TTTAGCACTG
CCAGACTTCT
CTGGAGAGGA
GGAGGTTTCT
GCCACTTTTC
AATTTCGAAC
TTGGAATAAG
GCAGGGCTTC
TGCTGTTCAG
TGAACTTCAG
CTGATTTCAG
GGGGTATCCT
CCTCTTTCTG
AGTGATGGAA
AACTTAAGTC
GAATCTCTAC
CAGCCAGGAA
AATTACCCAG
TGACATCACA
GCAGGTGTCG
AATTAAATGA
TGGGCAGTGG
CATTCTGTCT
CTTTATCTGC
TAAAAAGAAT
CACTTGAGTG
TGGCGGTGGA
CGGCCAGATG
GCTTCTGCTG
CTCCTCTGCT
GGGGCCTGAG
CAGATTTATT
CGGGTGGCAC
CTATTATTTT
GGAGGTTGTC
CTGACAAAAG
CTTTGGATCC
AAATGTAAAA
GTCCACTTGG
TGGATTTCAG
GGATGTATGA
GGCTCATTTC
TATCAGCGGC
AAAGTGGTAG
ATCTGATTTC
AGTTCAGCAG
GGGTCCCTTG
GGAACTTCAG
TGACCTTCAG
ATAGACTCAT
GTGGCATCTC
AGACAGGTGT
TTGCCCAACT
ATTGTGAACA
CGGTGGGGAG
TGTTCCCAGT
CCTGGAGCAC
CTTTCATTGT
AACTGTACCA
ACACTGGTTA
CAGAGGAGCT
ACTTGCCATA
ACTCTATCTA
TGAGCAGTCA
TGTGAAGCCT
ATAAGCACAG
AGGAAATACT
TCAGGGTTTT
ACTATATAGA
TTCAGATGGA
AGTGGTCCCC
TGGAACCATT
TCTTCTATAT
TGCAATATGA
CCGAAACTGC
ATGGACCATC
ATACAGCACA
ACGGCTCTGA
CTTAACAAGA
GTCAGAAATA
CTAATCCAGA
GAACCCATAT
GCTGGGTTTT
TCGAGTATGT
GGCCAGCATG
GAGCAACTTC
AGGCCACTAT
TAACCGTGCA
GAGCACTGTG
AACAGGAGTT
TACTTATTAC
TGCAAGAAGT
CACGGCTGGT
CAATAAGCAA
GATGGAACCC
CTCTGAGTTG
GTGGGTAGGA
AGAACCAATG
AAACGCAAAC
CTACTGGGGA
GGTTCTTCGC
CTGATCTTCA
AAAATGTACT
TGTGGATTAG
AGGGAAACTG
CATTGATTCT
CAGTATTACT
GCAATTGTGA
TGCTGACCGG
AATGAATGGA
CCAATGACAC
TGGATTGCTT
GCTTATAAAG
AACATCTTCC
AGTAACTAAG
ATCGTGATTA
CAGACACAGG
CCGACTGCAT
TCAGAAGCAG
CTTATAAACT
GGGGCCTCTG
CTCTGCCAGG
GAGACAGATC
ATTTTGGAAT
TCAGCTTCCT
TTGATACCGA
GGCTTCATAT
CTTCATTTTC
CTACCTTCCA
CGGAGAACTT
AGCGCGGATG
TATCTTTCTT
TTTTAAGACA
ACAGCTTCAT
CTGGGGTATT
TTTAGAGAAC
TTGGGGATTG
CTGATTTTAT
ACGGATAGAG
CTTCGCTCTC
CGACAGTAGT
GACTTTTTCA
TTTGATGTGG
GGAATGGGCC
TTTTGAAATC
TCAGTGCAGT
CACCCACCCA
CTTCAACGAC
AACCAGTGGC
ACCATGTGAG
GGTTGAAAGG
AACATGAAGG
AGGCCTCCCT
TCAAGTGGAT
CAGCTGACAC
CAAAGACACA
GCCCGCCCCC
GCTGATGGGC
ATGTCCTGTT
ACAGCTCAAC
AGTCAGCTCT
TCGTGGGTGG
AACGGCCACC
AGACAGAGAG
GCTTTCTGGG
CTGCATTCGG
TCTCTGCAGT
TGAATGGGAT
GACCCTGGAT
TTGGAAGAAA
GAGCCCAGGT
GACTCCAGAA
GTGCAGCCAG
GTTGTAGGGG
ACATTGCAGC
AGCTATGGGA
AGTTATGCCG
CAATGGAGGG
AAATGCAGAG
AAAGACCCAT
TGGGTTCTTT
TGTGACTGCA
CTTTCTCTGC
ATACACAGGG
CCATTCTGCT
CAAATGAGAT
TTCTGCATAT
TTTGGATCTG
GCTCATCCGT
GATATACAAT
TTTCAAGAAA
ATTATCTTTT
AAGTAAAAAC
TCCAGCTCCC
ACGCTGCTTC
ATTTCATGGT
GATATGAAGC
TGAGCAGAGA
AATGATCAAA
TTTAGTTTCC
GAACAACACG
AACACCAAGC
TTGCTGCTTT
TTGTGAGCTC
CTTTTACAAA
GAATACCTTT
CTGTGATCAT
TGCCAAAAAT
GGAAGTTTGC
AGATCAGGTA
CAAGTTAAAT
AAATATCAAG
AGCCTGATGT
TGTTAACTTT
GATTTTAAAA
ACATGGCTGA
TGGACAACTT
CACCACATAA
TGATTAACAG
AGAAGAAGGA
GTGGTCTTTA
TAGAGATTGA
CGATAATAGA
AGGAGACAAG
TTCACCTGTC
ATCAGGCACA
GAATTCAGTG
CAGTCAAATC
TCTGGTATTG
GGCAGGATTT
TAGAACACAG
TGATGTGGAC
CAGGATACTG
CACTGGCAGG
TGCGCAGGGC
TTCACAGGCT
GCCTCTCTGC
AGTGCAGCTC
AGCCACGTGG
CCCCTCTGAA
GGCAGCTCTG
CACCCCAGCC
ACCCAGACCC
TGTCACTGTT
ACAGGACACG
TGACTGAGTC
CAGCTGTATG
GCCCAGCCTG
GCACTGATGC
CACATCAAGG
GAAAGGACAC
ACTCGTTTGC
AGATCATTCT
GGAACAATAG
ATGACAGAGA
GCCCCTTGCT
AATGCAATCA
AAAGTGACTC
TGCAGTAATT
GGAGGTCTGA
TAGCTGTTGT
GATTTTTATC
TTGCTTTGCA
TCACTGCCAT
AGCTGTTCGC
ATTTATCAGC
AGAAAAGGTT
ATATAAAAGA
AGTGAGGCAA
AAAGGTCAGA
GAATGTAGAC
AGTGCTGAGG
CTGTTCTGAA
AAGTGAGCTT
AATATACAAA
ATGCAGTCAA
TGAAAATCAG
AAAGAGTACT
TCTTCCTCGA
GGAACAAAAA
CTCATCTCAG
AAGAGGATCT
GAATATGAAT
ACCGGTCATC
ATCACCATCA
CCATTGAGTT
TAAACCCGCT
GATCAGCCTC
GACTGTGCCT
TCTAGTTGCC
AGCCATCTGT
TGTTTGCCCC
TCCCCCGTGC
CTTCCTTGAC
CCTGGAAGGT
GCCACTCCCA
CTGTCCTTTC
CTAATAAAAT
GAGGAAATTG
CATCGCATTG
TCTGAGTAGG
TGTCATTCTA
TTCTGGGGGG
TGGGGTGGGG
CAGGACAGCA
AGGGGGAGGA
TTGGGAAGAC
AATAGCAGGC
ATGCTGGGGA
TGCGGTGGGC
TCTATGGCTT
CTGAGGCGGA
AAGAACCAGC
TGGGGCTCTA
GGGGGTATCC
CCACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGC
ATCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTAG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGTCTATT
CTTTTGATTT
ATAAGGGATT
TTGGGGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TAATTCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCTGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
CCCGGGAGCT
TGTATATCCA
TTTTCGGATC
TGATCAGCAC
GTGTTGACAA
TTAATCATCG
GCATAGTATA
TCGGCATAGT
ATAATACGAC
AAGGTGAGGA
ACTAAACCAT
GGCCAAGTTG
ACCAGTGCCG
TTCCGGTGCT
CACCGCGCGC
GACGTCGCCG
GAGCGGTCGA
GTTCTGGACC
GACCGGCTCG
GGTTCTCCCG
GGACTTCGTG
GAGGACGACT
TCGCCGGTGT
GGTCCGGGAC
GACGTGACCC
TGTTCATCAG
CGCGGTCCAG
GACCAGGTGG
TGCCGGACAA
CACCCTGGCC
TGGGTGTGGG
TGCGCGGCCT
GGACGAGCTG
TACGCCGAGT
GGTCGGAGGT
CGTGTCCACG
AACTTCCGGG
ACGCCTCCGG
GCCGGCCATG
ACCGAGATCG
GCGAGCAGCC
GTGGGGGCGG
GAGTTCGCCC
TGCGCGACCC
GGCCGGCAAC
TGCGTGCACT
TCGTGGCCGA
GGAGCAGGAC
TGACACGTGC
TACGAGATTT
CGATTCCACC
GCCGCCTTCT
ATGAAAGGTT
GGGCTTCGGA
ATCGTTTTCC
GGGACGCCGG
CTGGATGATC
CTCCAGCGCG
GGGATCTCAT
GCTGGAGTTC
TTCGCCCACC
CCAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGTATACCG
TCGACCTCTA
GCTAGAGCTT
GGCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAA
TGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC