名称: | pLV3-CMV-Tnf(mouse)-CopGFP-Puro |
---|---|
别称: | NM_013693.3;DIF; TNF-a; TNF-alpha; Tnfa; TNFalpha;Tnfsf1a; TNFSF2; Tnlg1f |
基因长: | 705bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGA
GCACAGAAAG
CATGATCCGC
GACGTGGAAC
TGGCAGAAGA
GGCACTCCCC
CAAAAGATGG
GGGGCTTCCA
GAACTCCAGG
CGGTGCCTAT
GTCTCAGCCT
CTTCTCATTC
CTGCTTGTGG
CAGGGGCCAC
CACGCTCTTC
TGTCTACTGA
ACTTCGGGGT
GATCGGTCCC
CAAAGGGATG
AGAAGTTCCC
AAATGGCCTC
CCTCTCATCA
GTTCTATGGC
CCAGACCCTC
ACACTCAGAT
CATCTTCTCA
AAATTCGAGT
GACAAGCCTG
TAGCCCACGT
CGTAGCAAAC
CACCAAGTGG
AGGAGCAGCT
GGAGTGGCTG
AGCCAGCGCG
CCAACGCCCT
CCTGGCCAAC
GGCATGGATC
TCAAAGACAA
CCAACTAGTG
GTGCCAGCCG
ATGGGTTGTA
CCTTGTCTAC
TCCCAGGTTC
TCTTCAAGGG
ACAAGGCTGC
CCCGACTACG
TGCTCCTCAC
CCACACCGTC
AGCCGATTTG
CTATCTCATA
CCAGGAGAAA
GTCAACCTCC
TCTCTGCCGT
CAAGAGCCCC
TGCCCCAAGG
ACACCCCTGA
GGGGGCTGAG
CTCAAACCCT
GGTATGAGCC
CATATACCTG
GGAGGAGTCT
TCCAGCTGGA
GAAGGGGGAC
CAACTCAGCG
CTGAGGTCAA
TCTGCCCAAG
TACTTAGACT
TTGCGGAGTC
CGGGCAGGTC
TACTTTGGAG
TCATTGCTCT
GTGAGGATCC
GACTACAAAG
ACCATGACGG
TGATTATAAA
GATCATGACA
TCGACTACAA
GGATGACGAT
GACAAGTAGC
GGCCGCGAAG
GATCTGCGAT
CGCTCCGGTG
CCCGTCAGTG
GGCAGAGCGC
ACATCGCCCA
CAGTCCCCGA
GAAGTTGGGG
GGAGGGGTCG
GCAATTGAAC
GGGTGCCTAG
AGAAGGTGGC
GCGGGGTAAA
CTGGGAAAGT
GATGTCGTGT
ACTGGCTCCG
CCTTTTTCCC
GAGGGTGGGG
GAGAACCGTA
TATAAGTGCA
GTAGTCGCCG
TGAACGTTCT
TTTTCGCAAC
GGGTTTGCCG
CCAGAACACA
GCTGAAGCTT
CGAGGGGCTC
GCATCTCTCC
TTCACGCGCC
CGCCGCCCTA
CCTGAGGCCG
CCATCCACGC
CGGTTGAGTC
GCGTTCTGCC
GCCTCCCGCC
TGTGGTGCCT
CCTGAACTGC
GTCCGCCGTC
TAGGTAAGTT
TAAAGCTCAG
GTCGAGACCG
GGCCTTTGTC
CGGCGCTCCC
TTGGAGCCTA
CCTAGACTCA
GCCGGCTCTC
CACGCTTTGC
CTGACCCTGC
TTGCTCAACT
CTACGTCTTT
GTTTCGTTTT
CTGTTCTGCG
CCGTTACAGA
TCCAAGCTGT
GACCGGCGCC
TACGCTAGAC
GCCACCATGG
AGAGCGACGA
GAGCGGCCTG
CCCGCCATGG
AGATCGAGTG
CCGCATCACC
GGCACCCTGA
ACGGCGTGGA
GTTCGAGCTG
GTGGGCGGCG
GAGAGGGCAC
CCCCAAGCAG
GGCCGCATGA
CCAACAAGAT
GAAGAGCACC
AAAGGCGCCC
TGACCTTCAG
CCCCTACCTG
CTGAGCCACG
TGATGGGCTA
CGGCTTCTAC
CACTTCGGCA
CCTACCCCAG
CGGCTACGAG
AACCCCTTCC
TGCACGCCAT
CAACAACGGC
GGCTACACCA
ACACCCGCAT
CGAGAAGTAC
GAGGACGGCG
GCGTGCTGCA
CGTGAGCTTC
AGCTACCGCT
ACGAGGCCGG
CCGCGTGATC
GGCGACTTCA
AGGTGGTGGG
CACCGGCTTC
CCCGAGGACA
GCGTGATCTT
CACCGACAAG
ATCATCCGCA
GCAACGCCAC
CGTGGAGCAC
CTGCACCCCA
TGGGCGATAA
CGTGCTGGTG
GGCAGCTTCG
CCCGCACCTT
CAGCCTGCGC
GACGGCGGCT
ACTACAGCTT
CGTGGTGGAC
AGCCACATGC
ACTTCAAGAG
CGCCATCCAC
CCCAGCATCC
TGCAGAACGG
GGGCCCCATG
TTCGCCTTCC
GCCGCGTGGA
GGAGCTGCAC
AGCAACACCG
AGCTGGGCAT
CGTGGAGTAC
CAGCACGCCT
TCAAGACCCC
CATCGCCTTC
GCCAGATCCC
GCGCTCAGTC
GTCCAATTCT
GCCGTGGACG
GCACCGCCGG
ACCCGGCTCC
ACCGGATCTC
GCGAGGGCAG
AGGAAGTCTT
CTAACATGCG
GTGACGTGGA
GGAGAATCCC
GGCCCTATGA
CCGAGTACAA
GCCCACGGTG
CGCCTCGCCA
CCCGCGACGA
CGTCCCCAGG
GCCGTACGCA
CCCTCGCCGC
CGCGTTCGCC
GACTACCCCG
CCACGCGCCA
CACCGTCGAT
CCGGACCGCC
ACATCGAGCG
GGTCACCGAG
CTGCAAGAAC
TCTTCCTCAC
GCGCGTCGGG
CTCGACATCG
GCAAGGTGTG
GGTCGCGGAC
GACGGCGCCG
CGGTGGCGGT
CTGGACCACG
CCGGAGAGCG
TCGAAGCGGG
GGCGGTGTTC
GCCGAGATCG
GCCCGCGCAT
GGCCGAGTTG
AGCGGTTCCC
GGCTGGCCGC
GCAGCAACAG
ATGGAAGGCC
TCCTGGCGCC
GCACCGGCCC
AAGGAGCCCG
CGTGGTTCCT
GGCCACCGTC
GGCGTCTCGC
CCGACCACCA
GGGCAAGGGT
CTGGGCAGCG
CCGTCGTGCT
CCCCGGAGTG
GAGGCGGCCG
AGCGCGCCGG
GGTGCCCGCC
TTCCTGGAGA
CCTCCGCGCC
CCGCAACCTC
CCCTTCTACG
AGCGGCTCGG
CTTCACCGTC
ACCGCCGACG
TCGAGGTGCC
CGAAGGACCG
CGCACCTGGT
GCATGACCCG
CAAGCCCGGT
GCCTGAAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AATCATCGTC
CTTTCCTTGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCCTGGT
ACCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GGGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGATAA
GATCTGCTTT
TTGCTTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGCTCTA
GCTATCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGACTTTTG
CAGAGACCAA
ATTCGTAATC
ATGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCATA
TGCGGTGTGA
AATACCGCAC
AGATGCGTAA
GGAGAAAATA
CCGCATCAGG
CGCCATTCGC
CATTCAGGCT
GCGCAACTGT
TGGGAAGGGC
GATCGGTGCG
GGCCTCTTCG
CTATTACGCC
AGCTGGCGAA
AGGGGGATGT
GCTGCAAGGC
GATTAAGTTG
GGTAACGCCA
GGGTTTTCCC
AGTCACGACG
TTGTAAAACG
ACGGCCAGTG
CCAAGCTG