名称: | pCMV-MYH9(human)-3×Myc-Neo |
---|---|
别称: | NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA;NMHC-II-A; NMMHC-IIA; NMMHCA; |
基因长: | 5880bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCDNA3.1-3×Myc-C |
正向引物: | BGH |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGTATCTG
CTCCCTGCTT
GTGTGTTGGA
GGTCGCTGAG
TAGTGCGCGA
GCAAAATTTA
AGCTACAACA
AGGCAAGGCT
TGACCGACAA
TTGCATGAAG
AATCTGCTTA
GGGTTAGGCG
TTTTGCGCTG
CTTCGCGATG
TACGGGCCAG
ATATACGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTCTCTG
GCTAACTAGA
GAACCCACTG
CTTACTGGCT
TATCGAAATT
AATACGACTC
ACTATAGGGA
GACCCAAGCT
GGCTAGCGTT
TAAACTTAAG
CTTGGTACCG
AGCTCGGATC
CACTAGTCCA
GTGTGGTGGA
ATTCGCCACC
ATGGCACAGC
AAGCTGCCGA
TAAGTATCTC
TATGTGGATA
AAAACTTCAT
CAACAATCCG
CTGGCCCAGG
CCGACTGGGC
TGCCAAGAAG
CTGGTATGGG
TGCCTTCCGA
CAAGAGTGGC
TTTGAGCCAG
CCAGCCTCAA
GGAGGAGGTG
GGCGAAGAGG
CCATCGTGGA
GCTGGTGGAG
AATGGGAAGA
AGGTGAAGGT
GAACAAGGAT
GACATCCAGA
AGATGAACCC
GCCCAAGTTC
TCCAAGGTGG
AGGACATGGC
AGAGCTCACG
TGCCTCAACG
AAGCCTCGGT
GCTGCACAAC
CTCAAGGAGC
GTTACTACTC
AGGGCTCATC
TACACCTATT
CAGGCCTGTT
CTGTGTGGTC
ATCAATCCTT
ACAAGAACCT
GCCCATCTAC
TCTGAAGAGA
TTGTGGAAAT
GTACAAGGGC
AAGAAGAGGC
ACGAGATGCC
CCCTCACATC
TATGCCATCA
CAGACACCGC
CTACAGGAGT
ATGATGCAAG
ACCGAGAAGA
TCAATCCATC
TTGTGCACTG
GTGAATCTGG
AGCTGGCAAG
ACGGAGAACA
CCAAGAAGGT
CATCCAGTAT
CTGGCGTACG
TGGCGTCCTC
GCACAAGAGC
AAGAAGGACC
AGGGCGAGCT
GGAGCGGCAG
CTGCTGCAGG
CCAACCCCAT
CCTGGAGGCC
TTCGGGAACG
CCAAGACCGT
GAAGAATGAC
AACTCCTCCC
GCTTCGGCAA
ATTCATTCGC
ATCAACTTTG
ATGTCAATGG
CTACATTGTT
GGAGCCAACA
TTGAGACTTA
TCTTTTGGAG
AAATCTCGTG
CTATCCGCCA
AGCCAAGGAA
GAACGGACCT
TCCACATCTT
CTATTATCTC
CTGTCTGGGG
CTGGAGAGCA
CCTGAAGACC
GATCTCCTGT
TGGAGCCGTA
CAACAAATAC
CGCTTCCTGT
CCAATGGACA
CGTCACCATC
CCCGGGCAGC
AGGACAAGGA
CATGTTCCAG
GAGACCATGG
AGGCCATGAG
GATTATGGGC
ATCCCAGAAG
AGGAGCAAAT
GGGCCTGCTG
CGGGTCATCT
CAGGGGTTCT
TCAGCTCGGC
AACATCGTCT
TCAAGAAGGA
GCGGAACACT
GACCAGGCGT
CCATGCCCGA
CAACACAGCT
GCCCAAAAGG
TGTCCCATCT
CTTGGGTATC
AATGTGACCG
ATTTCACCAG
AGGAATCCTC
ACCCCGCGCA
TCAAGGTGGG
ACGGGATTAC
GTCCAGAAGG
CGCAGACTAA
AGAGCAGGCT
GACTTTGCCA
TCGAGGCCTT
GGCCAAGGCG
ACCTATGAGC
GGATGTTCCG
CTGGCTGGTG
CTGCGCATCA
ACAAGGCTCT
GGACAAGACC
AAGAGGCAGG
GCGCCTCCTT
CATCGGGATC
CTGGACATTG
CCGGCTTCGA
GATCTTTGAT
CTGAACTCGT
TTGAGCAGCT
GTGCATCAAT
TACACCAATG
AGAAGCTGCA
GCAGCTCTTC
AACCACACCA
TGTTCATCCT
GGAGCAGGAG
GAGTACCAGC
GCGAGGGCAT
CGAGTGGAAC
TTCATCGACT
TTGGCCTCGA
CCTGCAGCCC
TGCATCGACC
TCATTGAGAA
GCCAGCAGGC
CCCCCGGGCA
TTCTGGCCCT
GCTGGACGAG
GAGTGCTGGT
TCCCCAAAGC
CACCGACAAG
AGCTTCGTGG
AGAAGGTGAT
GCAGGAGCAG
GGCACCCACC
CCAAGTTCCA
GAAGCCCAAG
CAGCTGAAGG
ACAAAGCTGA
TTTCTGCATT
ATCCACTATG
CCGGCAAGGT
GGATTACAAA
GCTGACGAGT
GGCTGATGAA
GAACATGGAT
CCCCTGAATG
ACAACATCGC
CACACTGCTC
CACCAGTCCT
CTGACAAGTT
TGTCTCGGAG
CTGTGGAAGG
ATGTGGACCG
CATCATCGGC
CTGGACCAGG
TGGCCGGCAT
GTCGGAGACC
GCACTGCCCG
GGGCCTTCAA
GACGCGGAAG
GGCATGTTCC
GCACTGTGGG
GCAGCTTTAC
AAGGAGCAGC
TGGCCAAGCT
GATGGCTACG
CTGAGGAACA
CGAACCCCAA
CTTTGTCCGC
TGCATCATCC
CCAACCACGA
GAAGAAGGCC
GGCAAGCTGG
ACCCGCATCT
CGTGCTGGAC
CAGCTGCGCT
GCAACGGTGT
TCTCGAGGGC
ATCCGTATCT
GCCGCCAGGG
CTTCCCCAAC
AGGGTGGTCT
TCCAGGAGTT
TCGGCAGAGA
TATGAGATCC
TGACTCCAAA
CTCCATTCCC
AAGGGTTTCA
TGGACGGGAA
GCAGGCGTGC
GTGCTCATGA
TAAAAGCCCT
GGAGCTCGAC
AGCAATCTGT
ACCGCATTGG
CCAGAGCAAA
GTCTTCTTCC
GTGCCGGTGT
GCTGGCCCAC
CTGGAGGAGG
AGCGAGACCT
GAAGATCACC
GACGTCATCA
TAGGGTTCCA
GGCCTGCTGC
AGGGGCTACC
TGGCCAGGAA
AGCATTTGCC
AAGCGGCAGC
AGCAGCTTAC
CGCCATGAAG
GTCCTCCAGC
GGAACTGCGC
TGCCTACCTG
AAGCTGCGGA
ACTGGCAGTG
GTGGCGGCTC
TTCACCAAGG
TCAAGCCGCT
GCTGCAGGTG
AGCCGGCAGG
AGGAGGAGAT
GATGGCCAAG
GAGGAGGAGC
TGGTGAAGGT
CAGAGAGAAG
CAGCTGGCTG
CGGAGAACAG
GCTCACGGAG
ATGGAGACGC
TGCAGTCTCA
GCTCATGGCA
GAGAAATTGC
AGCTGCAGGA
GCAGCTCCAG
GCAGAAACCG
AGCTGTGTGC
CGAGGCTGAG
GAGCTCCGGG
CCCGCCTGAC
CGCCAAGAAG
CAGGAATTAG
AAGAGATCTG
CCATGACCTA
GAGGCCAGGG
TGGAGGAGGA
GGAGGAGCGC
TGCCAGCACC
TGCAGGCGGA
GAAGAAGAAG
ATGCAGCAGA
ACATCCAGGA
GCTTGAGGAG
CAGCTGGAGG
AGGAGGAGAG
CGCCCGGCAG
AAGCTGCAGC
TGGAGAAGGT
GACCACCGAG
GCGAAGCTGA
AAAAGCTGGA
GGAGGAGCAG
ATCATCCTGG
AGGACCAGAA
CTGCAAGCTG
GCCAAGGAAA
AGAAACTGCT
GGAAGACAGA
ATAGCTGAGT
TCACCACCAA
CCTCACAGAA
GAGGAGGAGA
AATCTAAGAG
CCTCGCCAAG
CTCAAGAACA
AGCATGAGGC
AATGATCACT
GACTTGGAAG
AGCGCCTCCG
CAGGGAGGAG
AAGCAGCGAC
AGGAGCTGGA
GAAGACCCGC
CGGAAGCTGG
AGGGAGACTC
CACAGACCTC
AGCGACCAGA
TCGCCGAGCT
CCAGGCCCAG
ATCGCGGAGC
TCAAGATGCA
GCTGGCCAAG
AAAGAGGAGG
AGCTCCAGGC
CGCCCTGGCC
AGAGTGGAAG
AGGAAGCTGC
CCAGAAGAAC
ATGGCCCTCA
AGAAGATCCG
GGAGCTGGAA
TCTCAGATCT
CTGAACTCCA
GGAAGACCTG
GAGTCTGAGC
GTGCTTCCAG
GAATAAAGCT
GAGAAGCAGA
AACGGGACCT
TGGGGAAGAG
CTAGAGGCTC
TGAAAACAGA
GTTGGAGGAC
ACGCTGGATT
CCACAGCTGC
CCAGCAGGAG
CTCAGGTCAA
AACGTGAGCA
GGAGGTGAAC
ATCCTGAAGA
AGACCCTGGA
GGAGGAGGCC
AAGACCCACG
AGGCCCAGAT
CCAGGAGATG
AGGCAGAAGC
ACTCACAGGC
CGTGGAGGAG
CTGGCGGAGC
AGCTGGAGCA
GACGAAGCGG
GTGAAAGCAA
ACCTCGAGAA
GGCAAAGCAG
ACTCTGGAGA
ACGAGCGGGG
GGAGCTGGCC
AACGAGGTGA
AGGTGCTGCT
GCAGGGCAAA
GGGGACTCGG
AGCACAAGCG
CAAGAAAGTG
GAGGCGCAGC
TGCAGGAGCT
GCAGGTCAAG
TTCAACGAGG
GAGAGCGCGT
GCGCACAGAG
CTGGCCGACA
AGGTCACCAA
GCTGCAGGTG
GAGCTGGACA
ACGTGACCGG
GCTTCTCAGC
CAGTCCGACA
GCAAGTCCAG
CAAGCTCACC
AAGGACTTCT
CCGCGCTGGA
GTCCCAGCTG
CAGGACACTC
AGGAGCTGCT
GCAGGAGGAG
AACCGGCAGA
AGCTGAGCCT
GAGCACCAAG
CTCAAGCAGG
TGGAGGACGA
GAAGAATTCC
TTCCGGGAGC
AGCTGGAGGA
GGAGGAGGAG
GCCAAGCACA
ACCTGGAGAA
GCAGATCGCC
ACCCTCCATG
CCCAGGTGGC
CGACATGAAA
AAGAAGATGG
AGGACAGTGT
GGGGTGCCTG
GAAACTGCTG
AGGAGGTGAA
GAGGAAGCTC
CAGAAGGACC
TGGAGGGCCT
GAGCCAGCGG
CACGAGGAGA
AGGTGGCCGC
CTACGACAAG
CTGGAGAAGA
CCAAGACGCG
GCTGCAGCAG
GAGCTGGACG
ACCTGCTGGT
GGACCTGGAC
CACCAGCGCC
AGAGCGCGTG
CAACCTGGAG
AAGAAGCAGA
AGAAGTTTGA
CCAGCTCCTG
GCGGAGGAGA
AGACCATCTC
TGCCAAGTAT
GCAGAGGAGC
GCGACCGGGC
TGAGGCGGAG
GCCCGAGAGA
AGGAGACCAA
GGCTCTGTCG
CTGGCCCGGG
CCCTGGAGGA
AGCCATGGAG
CAGAAGGCGG
AGCTGGAGCG
GCTCAACAAG
CAGTTCCGCA
CGGAGATGGA
GGACCTTATG
AGCTCCAAGG
ATGATGTGGG
CAAGAGTGTC
CACGAGCTGG
AGAAGTCCAA
GCGGGCCCTA
GAGCAGCAGG
TGGAGGAGAT
GAAGACGCAG
CTGGAAGAGC
TGGAGGACGA
GCTGCAGGCC
ACCGAAGATG
CCAAGCTGCG
GTTGGAGGTC
AACCTGCAGG
CCATGAAGGC
CCAGTTCGAG
CGGGACCTGC
AGGGCCGGGA
CGAGCAGAGC
GAGGAGAAGA
AGAAGCAGCT
GGTCAGACAG
GTGCGGGAGA
TGGAGGCAGA
GCTGGAGGAC
GAGAGGAAGC
AGCGCTCGAT
GGCAGTGGCC
GCCCGGAAGA
AGCTGGAGAT
GGACCTGAAG
GACCTGGAGG
CGCACATCGA
CTCGGCCAAC
AAGAACCGGG
ACGAAGCCAT
CAAACAGCTG
CGGAAGCTGC
AGGCCCAGAT
GAAGGACTGC
ATGCGCGAGC
TGGATGACAC
CCGCGCCTCT
CGTGAGGAGA
TCCTGGCCCA
GGCCAAAGAG
AACGAGAAGA
AGCTGAAGAG
CATGGAGGCC
GAGATGATCC
AGTTGCAGGA
GGAACTGGCA
GCCGCGGAGC
GTGCCAAGCG
CCAGGCCCAG
CAGGAGCGGG
ATGAGCTGGC
TGACGAGATC
GCCAACAGCA
GCGGCAAAGG
AGCCCTGGCG
TTAGAGGAGA
AGCGGCGTCT
GGAGGCCCGC
ATCGCCCAGC
TGGAGGAGGA
GCTGGAGGAG
GAGCAGGGCA
ACACGGAGCT
GATCAACGAC
CGGCTGAAGA
AGGCCAACCT
GCAGATCGAC
CAGATCAACA
CCGACCTGAA
CCTGGAGCGC
AGCCACGCCC
AGAAGAACGA
GAATGCTCGG
CAGCAGCTGG
AACGCCAGAA
CAAGGAGCTT
AAGGTCAAGC
TGCAGGAGAT
GGAGGGCACT
GTCAAGTCCA
AGTACAAGGC
CTCCATCACC
GCCCTCGAGG
CCAAGATTGC
ACAGCTGGAG
GAGCAGCTGG
ACAACGAGAC
CAAGGAGCGC
CAGGCAGCCT
GCAAACAGGT
GCGTCGGACC
GAGAAGAAGC
TGAAGGATGT
GCTGCTGCAG
GTGGATGACG
AGCGGAGGAA
CGCCGAGCAG
TACAAGGACC
AGGCCGACAA
GGCATCTACC
CGCCTGAAGC
AGCTCAAGCG
GCAGCTGGAG
GAGGCCGAAG
AGGAGGCCCA
GCGGGCCAAC
GCCTCCCGCC
GGAAACTGCA
GCGCGAGCTG
GAGGACGCCA
CTGAGACGGC
CGATGCCATG
AACCGCGAAG
TCAGCTCCCT
AAAGAACAAG
CTCAGGCGCG
GGGACCTGCC
GTTTGTCGTG
CCCCGCCGAA
TGGCCCGGAA
AGGCGCCGGG
GATGGCTCCG
ACGAAGAGGT
AGATGGCAAA
GCGGATGGGG
CTGAGGCCAA
ACCTGCCGAA
CTCGAGGAAC
AAAAACTAAT
ATCAGAGGAA
GATTTGGAAC
AGAAACTAAT
CAGTGAAGAA
GATTTAGAGC
AAAAGTTAAT
TTCCGAGGAG
GACTTATGAG
GGCCCGTTTA
AACCCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGACAA
TAGCAGGCAT
GCTGGGGATG
CGGTGGGCTC
TATGGCTTCT
GAGGCGGAAA
GAACCAGCTG
GGGCTCTAGG
GGGTATCCCC
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTA
ATTCTGTGGA
ATGTGTGTCA
GTTAGGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCAGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAT
AGTCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CTGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGCTTTTGCA
AAAAGCTCCC
GGGAGCTTGT
ATATCCATTT
TCGGATCTGA
TCAAGAGACA
GGATGAGGAT
CGTTTCGCAT
GATTGAACAA
GATGGATTGC
ACGCAGGTTC
TCCGGCCGCT
TGGGTGGAGA
GGCTATTCGG
CTATGACTGG
GCACAACAGA
CAATCGGCTG
CTCTGATGCC
GCCGTGTTCC
GGCTGTCAGC
GCAGGGGCGC
CCGGTTCTTT
TTGTCAAGAC
CGACCTGTCC
GGTGCCCTGA
ATGAACTGCA
GGACGAGGCA
GCGCGGCTAT
CGTGGCTGGC
CACGACGGGC
GTTCCTTGCG
CAGCTGTGCT
CGACGTTGTC
ACTGAAGCGG
GAAGGGACTG
GCTGCTATTG
GGCGAAGTGC
CGGGGCAGGA
TCTCCTGTCA
TCTCACCTTG
CTCCTGCCGA
GAAAGTATCC
ATCATGGCTG
ATGCAATGCG
GCGGCTGCAT
ACGCTTGATC
CGGCTACCTG
CCCATTCGAC
CACCAAGCGA
AACATCGCAT
CGAGCGAGCA
CGTACTCGGA
TGGAAGCCGG
TCTTGTCGAT
CAGGATGATC
TGGACGAAGA
GCATCAGGGG
CTCGCGCCAG
CCGAACTGTT
CGCCAGGCTC
AAGGCGCGCA
TGCCCGACGG
CGAGGATCTC
GTCGTGACCC
ATGGCGATGC
CTGCTTGCCG
AATATCATGG
TGGAAAATGG
CCGCTTTTCT
GGATTCATCG
ACTGTGGCCG
GCTGGGTGTG
GCGGACCGCT
ATCAGGACAT
AGCGTTGGCT
ACCCGTGATA
TTGCTGAAGA
GCTTGGCGGC
GAATGGGCTG
ACCGCTTCCT
CGTGCTTTAC
GGTATCGCCG
CTCCCGATTC
GCAGCGCATC
GCCTTCTATC
GCCTTCTTGA
CGAGTTCTTC
TGAGCGGGAC
TCTGGGGTTC
GAAATGACCG
ACCAAGCGAC
GCCCAACCTG
CCATCACGAG
ATTTCGATTC
CACCGCCGCC
TTCTATGAAA
GGTTGGGCTT
CGGAATCGTT
TTCCGGGACG
CCGGCTGGAT
GATCCTCCAG
CGCGGGGATC
TCATGCTGGA
GTTCTTCGCC
CACCCCAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGTAT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CGACGGATCG
GGAGATCTCC
CGATCCCCTA
TGGT