名称: | pCMV-EGFP-Sf3b1(mouse-Toxic-2-opt)-3×FLAG-Neo |
---|---|
别称: | NM_031179.2;155kDa/2810001M05Rik/AA409119/Prp10/SAP155/SF3b155/TA-8/Targ4 |
基因长: | 3912bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-EGFP-Sf3b1(mouse-Toxic-opt)-3×FLAG-Neo |
正向引物: | |
反向引物: | BGH-R |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGCCA
CCATGGTGAG
CAAGGGCGAG
GAGCTGTTCA
CCGGGGTGGT
GCCCATCCTG
GTCGAGCTGG
ACGGCGACGT
AAACGGCCAC
AAGTTCAGCG
TGTCCGGCGA
GGGCGAGGGC
GATGCCACCT
ACGGCAAGCT
GACCCTGAAG
TTCATCTGCA
CCACCGGCAA
GCTGCCCGTG
CCCTGGCCCA
CCCTCGTGAC
CACCCTGACC
TACGGCGTGC
AGTGCTTCAG
CCGCTACCCC
GACCACATGA
AGCAGCACGA
CTTCTTCAAG
TCCGCCATGC
CCGAAGGCTA
CGTCCAGGAG
CGCACCATCT
TCTTCAAGGA
CGACGGCAAC
TACAAGACCC
GCGCCGAGGT
GAAGTTCGAG
GGCGACACCC
TGGTGAACCG
CATCGAGCTG
AAGGGCATCG
ACTTCAAGGA
GGACGGCAAC
ATCCTGGGGC
ACAAGCTGGA
GTACAACTAC
AACAGCCACA
ACGTCTATAT
CATGGCCGAC
AAGCAGAAGA
ACGGCATCAA
GGTGAACTTC
AAGATCCGCC
ACAACATCGA
GGACGGCAGC
GTGCAGCTCG
CCGACCACTA
CCAGCAGAAC
ACCCCCATCG
GCGACGGCCC
CGTGCTGCTG
CCCGACAACC
ACTACCTGAG
CACCCAGTCC
GCCCTGAGCA
AAGACCCCAA
CGAGAAGCGC
GATCACATGG
TCCTGCTGGA
GTTCGTGACC
GCCGCCGGGA
TCACTCTCGG
CATGGACGAG
CTGTACAAGG
GCGGTGGAGG
CAGTGGTGGA
GGCGGTAGCG
AATTCATGGC
GAAGATCGCC
AAGACTCACG
AAGATATCGA
AGCACAGATT
CGAGAAATTC
AAGGCAAGAA
GGCAGCTCTT
GATGAAGCCC
AAGGAGTGGG
CCTTGATTCC
ACAGGTTATT
ATGACCAAGA
AATTTATGGT
GGAAGTGATA
GCAGATTTGC
TGGATATGTG
ACATCAATTG
CTGCAACTGA
ACTTGAAGAT
GATGACGATG
ACTACTCATC
ATCCACGAGC
TTGCTCGGTC
AGAAGAAGCC
TGGATATCAT
GCCCCCGTGG
CGTTGCTTAA
TGATATACCA
CAGTCAACAG
AGCAGTATGA
TCCATTTGCT
GAGCATCGTC
CTCCAAAGAT
TGCCGATCGG
GAAGATGAAT
ACAAAAAGCA
TAGGCGGACC
ATGATAATTT
CCCCAGAGCG
TCTTGATCCT
TTTGCAGATG
GAGGGAAGAC
TCCCGATCCT
AAAATGAATG
CTAGAACCTA
CATGGATGTT
ATGCGAGAAC
AACATTTGAC
TAAGGAAGAG
AGAGAAATTA
GGCAACAACT
AGCAGAAAAA
GCTAAAGCTG
GAGAACTAAA
AGTCGTCAAT
GGAGCAGCAG
CATCCCAGCC
TCCCTCAAAA
CGAAAACGGC
GCTGGGATCA
GACCGCTGAC
CAGACTCCTG
GTGCCACTCC
CAAAAAGCTA
TCGAGTTGGG
ATCAGGCAGA
GACCCCTGGG
CATACCCCAT
CTTTAAGATG
GGATGAGACA
CCGGGTCGTG
CAAAAGGAAG
TGAAACACCT
GGCGCAACCC
CAGGCTCAAA
AATATGGGAC
CCTACACCGA
GTCATACACC
TGCGGGAGCT
GCTACTCCTG
GGCGAGGCGA
CACACCAGGC
CATGCAACCC
CGGGCCATGG
TGGTGCAACT
TCCAGTGCTC
GTAAAAACAG
ATGGGATGAG
ACCCCCAAAA
CAGAAAGAGA
TACTCCTGGG
CATGGAAGTG
GATGGGCTGA
GACTCCTCGA
ACAGATCGAG
GTGGAGACTC
TATTGGTGAA
ACACCAACTC
CTGGAGCAAG
TAAAAGAAAG
TCTCGTTGGG
ATGAAACGCC
GGCGAGTCAA
ATGGGTGGAA
GCACTCCTGT
TCTGACTCCA
GGAAAAACAC
CAATTGGCAC
ACCAGCCATG
AACATGGCTA
CCCCGACTCC
AGGTCACATA
ATGAGCATGA
CACCTGAACA
GCTTCAGGCG
TGGCGGTGGG
AGAGAGAAAT
TGATGAACGA
AACCGCCCAC
TTTCTGATGA
AGAATTAGAT
GCTATGTTCC
CAGAAGGATA
TAAGGTACTT
CCCCCTCCAG
CTGGTTATGT
TCCTATTCGA
ACTCCAGCTC
GAAAGCTGAC
AGCAACTCCT
ACACCTTTGG
GTGGTATGAC
TGGTTTCCAC
ATGCAAACTG
AAGACAGAAC
TATGAAAAGT
GTCAATGACC
AGCCATCTGG
GAATCTTCCA
TTTTTAAAAC
CTGATGACAT
TCAGTACTTT
GATAAACTAT
TGGTTGATGT
AGATGAATCA
ACACTTAGTC
CAGAAGAACA
AAAAGAAAGA
AAAATAATGA
AGTTGCTTTT
AAAAATTAAG
AATGGTACAC
CTCCAATGAG
AAAGGCTGCC
TTACGTCAGA
TTACTGATAA
AGCTCGAGAA
TTTGGAGCTG
GACCACTGTT
TAACCAGATT
CTTCCTCTGT
TGATGTCTCC
AACACTCGAG
GATCAGGAAA
GACACCTCCT
TGTGAAGGTC
ATCGACCGCA
TCCTGTATAA
GCTCGATGAC
CTCGTGCGGC
CATATGTCCA
TAAGATCCTT
GTGGTTATCG
AGCCCCTTCT
GATCGACGAG
GATTACTATG
CACGCGTTGA
GGGGCGGGAG
ATAATTAGCA
ACTTGGCAAA
GGCTGCAGGC
CTTGCTACCA
TGATCTCTAC
TATGCGGCCG
GATATCGACA
ACATGGACGA
ATACGTGAGA
AACACAACTG
CCCGAGCATT
TGCCGTGGTC
GCTTCTGCAC
TCGGAATACC
TAGTCTGCTG
CCCTTTCTGA
AAGCCGTGTG
TAAGAGTAAG
AAAAGCTGGC
AAGCACGGCA
CACCGGTATA
AAGATCGTCC
AGCAGATAGC
CATCCTCATG
GGTTGTGCCA
TCCTGCCACA
CCTTAGGAGC
CTGGTCGAAA
TTATTGAGCA
CGGTCTCGTC
GACGAGCAGC
AGAAGGTAAG
AACGATCAGT
GCCCTGGCAA
TAGCTGCCCT
GGCAGAGGCT
GCTACCCCAT
ATGGGATAGA
GTCCTTTGAT
TCAGTCCTGA
AGCCTCTGTG
GAAGGGAATC
AGACAACACC
GCGGGAAGGG
ACTTGCAGCC
TTTCTTAAAG
CTATCGGCTA
CTTGATACCG
CTCATGGACG
CCGAGTACGC
CAACTACTAC
ACCCGGGAGG
TGATGCTGAT
ACTTATAAGG
GAATTTCAGT
CACCTGATGA
AGAAATGAAA
AAAATTGTCC
TTAAGGTCGT
GAAGCAATGC
TGTGGTACTG
ACGGAGTGGA
AGCAAACTAC
ATTAAGACCG
AGATCCTGCC
GCCTTTTTTC
AAACACTTCT
GGCAGCATCG
AATGGCTTTG
GACAGGAGAA
ATTACCGGCA
GCTCGTCGAC
ACCACAGTGG
AGCTTGCAAA
TAAGGTGGGT
GCTGCCGAAA
TAATCAGCCG
CATCGTGGAC
GATCTCAAGG
ATGAGGCCGA
ACAGTACCGG
AAGATGGTGA
TGGAGACTAT
CGAGAAGATT
ATGGGAAACC
TGGGAGCTGC
TGACATAGAT
CATAAGCTGG
AGGAGCAGCT
TATAGACGGA
ATTCTGTACG
CTTTCCAGGA
GCAGACCACA
GAGGACTCTG
TTATGCTCAA
TGGCTTTGGA
ACTGTGGTTA
ACGCCCTGGG
AAAGCGAGTC
AAACCATATC
TCCCGCAGAT
TTGCGGTACC
GTTTTGTGGA
GACTGAACAA
CAAGTCCGCA
AAGGTCCGGC
AGCAAGCTGC
TGACCTGATC
AGCCGCACTG
CTGTCGTTAT
GAAGACGTGC
CAAGAAGAGA
AACTCATGGG
CCATTTGGGG
GTTGTGTTGT
ACGAGTACCT
TGGGGAGGAG
TATCCCGAGG
TTTTGGGATC
CATCCTTGGC
GCTCTGAAGG
CAATAGTCAA
CGTGATCGGC
ATGCACAAAA
TGACTCCTCC
TATCAAAGAC
CTGCTGCCCC
GCCTGACCCC
AATCTTGAAA
AATCGCCACG
AGAAAGTCCA
GGAAAATTGC
ATCGACCTTG
TTGGAAGAAT
CGCTGACAGA
GGAGCCGAAT
ATGTAAGCGC
AAGGGAATGG
ATGAGAATCT
GCTTTGAGCT
CCTCGAGCTG
CTGAAAGCTC
ACAAGAAGGC
CATTAGGCGA
GCTACTGTGA
ACACCTTCGG
CTACATTGCA
AAGGCCATCG
GACCTCATGA
TGTTCTCGCC
ACCCTGCTCA
ATAACCTGAA
GGTACAAGAG
AGACAAAACA
GAGTATGTAC
CACAGTGGCC
ATCGCAATCG
TCGCTGAAAC
GTGTAGCCCA
TTCACAGTGC
TGCCAGCTTT
GATGAACGAG
TATCGGGTAC
CGGAGCTGAA
CGTTCAAAAC
GGGGTGCTGA
AGAGCCTGAG
CTTCCTTTTC
GAGTACATCG
GCGAGATGGG
CAAGGACTAC
ATTTATGCTG
TAACACCCTT
GCTCGAGGAT
GCTCTCATGG
ACCGAGATCT
TGTCCACAGG
CAGACAGCTA
GCGCTGTTGT
CCAGCATATG
AGCCTCGGTG
TATACGGATT
CGGATGCGAG
GATTCTCTTA
ACCACCTGTT
GAATTATGTT
TGGCCGAACG
TATTCGAAAC
CAGTCCACAC
GTTATCCAAG
CCGTGATGGG
TGCTTTGGAA
GGCCTGCGAG
TGGCCATCGG
CCCCTGTAGA
ATGCTGCAGT
ACTGTTTGCA
GGGTCTCTTC
CACCCCGCTA
GGAAGGTTAG
AGACGTGTAT
TGGAAGATCT
ACAACAGCAT
CTACATTGGC
TCCCAAGACG
CCCTCATCGC
CCACTACCCT
CGAATCTATA
ACGACGACAA
GAACACCTAC
ATCCGATATG
AGCTGGACTA
TATTCTTCTC
GAGGACTACA
AAGACCATGA
CGGTGATTAT
AAAGATCATG
ACATCGACTA
CAAGGATGAC
GATGACAAGT
AGTGAGGGCC
CGTTTAAACC
CGCTGATCAG
CCTCGACTGT
GCCTTCTAGT
TGCCAGCCAT
CTGTTGTTTG
CCCCTCCCCC
GTGCCTTCCT
TGACCCTGGA
AGGTGCCACT
CCCACTGTCC
TTTCCTAATA
AAATGAGGAA
ATTGCATCGC
ATTGTCTGAG
TAGGTGTCAT
TCTATTCTGG
GGGGTGGGGT
GGGGCAGGAC
AGCAAGGGGG
AGGATTGGGA
AGACAATAGC
AGGCATGCTG
GGGATGCGGT
GGGCTCTATG
GCTTCTGAGG
CGGAAAGAAC
CAGCTGGGGC
TCTAGGGGGT
ATCCCCACGC
GCCCTGTAGC
GGCGCATTAA
GCGCGGCGGG
TGTGGTGGTT
ACGCGCAGCG
TGACCGCTAC
ACTTGCCAGC
GCCCTAGCGC
CCGCTCCTTT
CGCTTTCTTC
CCTTCCTTTC
TCGCCACGTT
CGCCGGCTTT
CCCCGTCAAG
CTCTAAATCG
GGGGCTCCCT
TTAGGGTTCC
GATTTAGTGC
TTTACGGCAC
CTCGACCCCA
AAAAACTTGA
TTAGGGTGAT
GGTTCACGTA
GTGGGCCATC
GCCCTGATAG
ACGGTTTTTC
GCCCTTTGAC
GTTGGAGTCC
ACGTTCTTTA
ATAGTGGACT
CTTGTTCCAA
ACTGGAACAA
CACTCAACCC
TATCTCGGTC
TATTCTTTTG
ATTTATAAGG
GATTTTGCCG
ATTTCGGCCT
ATTGGTTAAA
AAATGAGCTG
ATTTAACAAA
AATTTAACGC
GAATTAATTC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCTGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTCCCGGGA
GCTTGTATAT
CCATTTTCGG
ATCTGATCAA
GAGACAGGAT
GAGGATCGTT
TCGCATGATT
GAACAAGATG
GATTGCACGC
AGGTTCTCCG
GCCGCTTGGG
TGGAGAGGCT
ATTCGGCTAT
GACTGGGCAC
AACAGACAAT
CGGCTGCTCT
GATGCCGCCG
TGTTCCGGCT
GTCAGCGCAG
GGGCGCCCGG
TTCTTTTTGT
CAAGACCGAC
CTGTCCGGTG
CCCTGAATGA
ACTGCAGGAC
GAGGCAGCGC
GGCTATCGTG
GCTGGCCACG
ACGGGCGTTC
CTTGCGCAGC
TGTGCTCGAC
GTTGTCACTG
AAGCGGGAAG
GGACTGGCTG
CTATTGGGCG
AAGTGCCGGG
GCAGGATCTC
CTGTCATCTC
ACCTTGCTCC
TGCCGAGAAA
GTATCCATCA
TGGCTGATGC
AATGCGGCGG
CTGCATACGC
TTGATCCGGC
TACCTGCCCA
TTCGACCACC
AAGCGAAACA
TCGCATCGAG
CGAGCACGTA
CTCGGATGGA
AGCCGGTCTT
GTCGATCAGG
ATGATCTGGA
CGAAGAGCAT
CAGGGGCTCG
CGCCAGCCGA
ACTGTTCGCC
AGGCTCAAGG
CGCGCATGCC
CGACGGCGAG
GATCTCGTCG
TGACCCATGG
CGATGCCTGC
TTGCCGAATA
TCATGGTGGA
AAATGGCCGC
TTTTCTGGAT
TCATCGACTG
TGGCCGGCTG
GGTGTGGCGG
ACCGCTATCA
GGACATAGCG
TTGGCTACCC
GTGATATTGC
TGAAGAGCTT
GGCGGCGAAT
GGGCTGACCG
CTTCCTCGTG
CTTTACGGTA
TCGCCGCTCC
CGATTCGCAG
CGCATCGCCT
TCTATCGCCT
TCTTGACGAG
TTCTTCTGAG
CGGGACTCTG
GGGTTCGAAA
TGACCGACCA
AGCGACGCCC
AACCTGCCAT
CACGAGATTT
CGATTCCACC
GCCGCCTTCT
ATGAAAGGTT
GGGCTTCGGA
ATCGTTTTCC
GGGACGCCGG
CTGGATGATC
CTCCAGCGCG
GGGATCTCAT
GCTGGAGTTC
TTCGCCCACC
CCAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGTATACCG
TCGACCTCTA
GCTAGAGCTT
GGCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGAACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTC