名称: | CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA |
---|---|
别称: | Plasmid#171380 |
基因长: | 2898bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 红色 |
启动子: | CMV, U6 |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCX539 |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCGTGCCCCG
CTCCGCCGCC
GCCTCGCGCC
GCCCGCCCCG
GCTCTGACTG
ACCGCGTTAC
TCCCACAGGT
GAGCGGGCGG
GACGGCCCTT
CTCCTCCGGG
CTGTAATTAG
CTGAGCAAGA
GGTAAGGGTT
TAAGGGATGG
TTGGTTGGTG
GGGTATTAAT
GTTTAATTAC
CTGGAGCACC
TGCCTGAAAT
CACTTTTTTT
CAGGTTGGAC
CGGTGCCACC
ATGCCTAAAA
AGAAAAGAAA
GGTGGGTTCT
GGTATCGAGA
AGAAGAAGAG
CTTCGCCAAG
GGCATGGGAG
TGAAGAGCAC
CCTGGTGTCC
GGCTCTAAGG
TGTACATGAC
CACATTTGCT
GAGGGAAGCG
ACGCCAGGCT
GGAGAAGATC
GTGGAGGGCG
ATAGCATCAG
ATCCGTGAAC
GAGGGAGAGG
CTTTCAGCGC
CGAGATGGCT
GACAAGAACG
CTGGCTACAA
GATCGGAAAC
GCCAAGTTTT
CCCACCCAAA
GGGCTACGCC
GTGGTGGCTA
ACAACCCACT
GTACACCGGA
CCAGTGCAGC
AGGACATGCT
GGGACTGAAG
GAGACACTGG
AGAAGAGGTA
CTTCGGCGAG
TCCGCCGACG
GAAACGATAA
CATCTGCATC
CAGGTCATCC
ACAACATCCT
GGATATCGAG
AAGATCCTGG
CTGAGTACAT
CACAAACGCC
GCTTACGCCG
TGAACAACAT
CTCCGGCCTG
GACAAGGATA
TCATCGGCTT
CGGAAAGTTT
TCTACCGTGT
ACACATACGA
CGAGTTCAAG
GATCCAGAGC
ACCACCGGGC
CGCTTTTAAC
AACAACGACA
AGCTGATCAA
CGCCATCAAG
GCTCAGTACG
ACGAGTTCGA
TAACTTTCTG
GATAACCCCA
GGCTGGGCTA
CTTCGGACAG
GCTTTCTTTT
CTAAGGAGGG
CAGAAACTAC
ATCATCAACT
ACGGAAACGA
GTGTTACGAC
ATCCTGGCCC
TGCTGAGCGG
ACTGAGGCAC
TGGGTGGTGC
ACAACAACGA
GGAGGAGTCT
CGGATCAGCC
GCACCTGGCT
GTACAACCTG
GACAAGAACC
TGGATAACGA
GTACATCTCC
ACACTGAACT
ACCTGTACGA
CAGGATCACC
AACGAGCTGA
CAAACAGCTT
CTCCAAGAAC
TCTGCCGCTA
ACGTGAACTA
CATCGCTGAG
ACCCTGGGCA
TCAACCCAGC
TGAGTTCGCT
GAGCAGTACT
TCAGATTTTC
CATCATGAAG
GAGCAGAAGA
ACCTGGGCTT
CAACATCACA
AAGCTGAGAG
AAGTGATGCT
GGACAGAAAG
GATATGTCCG
AGATCAGGAA
GAACCACAAG
GTGTTCGATT
CTATCAGAAC
CAAGGTGTAC
ACAATGATGG
ACTTTGTGAT
CTACAGGTAC
TACATCGAGG
AGGATGCCAA
GGTGGCCGCT
GCCAACAAGA
GCCTGCCCGA
CAACGAGAAG
TCTCTGAGCG
AGAAGGATAT
CTTCGTGATC
AACCTGAGAG
GCTCCTTTAA
CGACGATCAG
AAGGACGCTC
TGTACTACGA
TGAGGCCAAC
AGGATCTGGA
GAAAGCTGGA
GAACATCATG
CACAACATCA
AGGAGTTCCG
GGGAAACAAG
ACCCGCGAGT
ACAAGAAGAA
GGACGCTCCA
AGGCTGCCTA
GGATCCTGCC
TGCTGGAAGG
GACGTGAGCG
CCTTCAGCAA
GCTGATGTAC
GCCCTGACAA
TGTTTCTGGA
CGGAAAGGAG
ATCAACGATC
TGCTGACCAC
ACTGATCAAC
AAGTTCGACA
ACATCCAGTC
TTTTCTGAAA
GTGATGCCTC
TGATCGGCGT
GAACGCTAAG
TTCGTGGAGG
AGTACGCCTT
CTTTAAGGAC
AGCGCCAAGA
TCGCTGATGA
GCTGCGGCTG
ATCAAGTCCT
TTGCCAGGAT
GGGAGAGCCA
ATCGCTGACG
CTAGGAGAGC
TATGTACATC
GATGCCATCC
GGATCCTGGG
AACCAACCTG
TCTTACGACG
AGCTGAAGGC
TCTGGCCGAC
ACCTTCAGCC
TGGATGAGAA
CGGCAACAAG
CTGAAGAAGG
GCAAGCACGG
AATGCGCAAC
TTCATCATCA
ACAACGTGAT
CAGCAACAAG
CGGTTTCACT
ACCTGATCAG
ATACGGCGAC
CCAGCTCACC
TGCACGAGAT
CGCTAAGAAC
GAGGCCGTGG
TGAAGTTCGT
GCTGGGACGG
ATCGCCGATA
TCCAGAAGAA
GCAGGGCCAG
AACGGAAAGA
ACCAGATCGA
CCGCTACTAC
GAGACCTGCA
TCGGCAAGGA
TAAGGGAAAG
TCCGTGTCTG
AGAAGGTGGA
CGCTCTGACC
AAGATCATCA
CAGGCATGAA
CTACGACCAG
TTCGATAAGA
AGAGATCTGT
GATCGAGGAC
ACCGGAAGGG
AGAACGCCGA
GAGAGAGAAG
TTTAAGAAGA
TCATCAGCCT
GTACCTGACA
GTGATCTACC
ACATCCTGAA
GAACATCGTG
AACATCAACG
CTAGATACGT
GATCGGCTTC
CACTGCGTGG
AGCGCGATGC
CCAGCTGTAC
AAGGAGAAGG
GATACGACAT
CAACCTGAAG
AAGCTGGAGG
AGAAGGGCTT
TAGCTCCGTG
ACCAAGCTGT
GCGCTGGAAT
CGACGAGACA
GCCCCCGACA
AGAGGAAGGA
TGTGGAGAAG
GAGATGGCCG
AGAGAGCTAA
GGAGAGCATC
GACTCCCTGG
AGTCTGCTAA
CCCTAAGCTG
TACGCCAACT
ACATCAAGTA
CTCCGATGAG
AAGAAGGCCG
AGGAGTTCAC
CAGGCAGATC
AACAGAGAGA
AGGCCAAGAC
CGCTCTGAAC
GCCTACCTGA
GGAACACAAA
GTGGAACGTG
ATCATCCGGG
AGGACCTGCT
GCGCATCGAT
AACAAGACCT
GTACACTGTT
CCGGAACAAG
GCTGTGCACC
TGGAGGTGGC
TCGCTACGTG
CACGCCTACA
TCAACGACAT
CGCCGAGGTG
AACTCCTACT
TTCAGCTGTA
CCACTACATC
ATGCAGAGGA
TCATCATGAA
CGAGAGATAC
GAGAAGTCTA
GCGGCAAGGT
GTCTGAGTAC
TTCGACGCCG
TGAACGATGA
GAAGAAGTAC
AACGATAGAC
TGCTGAAGCT
GCTGTGCGTG
CCTTTCGGAT
ACTGTATCCC
ACGGTTTAAG
AACCTGAGCA
TCGAGGCCCT
GTTCGACCGC
AACGAGGCTG
CCAAGTTTGA
TAAGGAGAAG
AAGAAGGTGA
GCGGCAACTC
CGGTTCTGGT
TACCCATACG
ACGTACCAGA
TTACGCTCCC
AAGAAGAAGC
GCAAGGTGGG
ATAAAAGCTT
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGATCACTAG
TGAGGGCCTA
TTTCCCATGA
TTCCTTCATA
TTTGCATATA
CGATACAAGG
CTGTTAGAGA
GATAATTGGA
ATTAATTTGA
CTGTAAACAC
AAAGATATTA
GTACAAAATA
CGTGACGTAG
AAAGTAATAA
TTTCTTGGGT
AGTTTGCAGT
TTTAAAATTA
TGTTTTAAAA
TGGACTATCA
TATGCTTACC
GTAACTTGAA
AGTATTTCGA
TTTCTTGGCT
TTATATATCT
TGTGGAAAGG
ACGAAACACC
GCAAGTAAAC
CCCTACCAAC
TGGTCGGGGT
TTGAAACGGG
TCTTCGAGAA
GACCTTTTTT
TTACGCGTTA
GTTATTAATA
GTAATCAATT
ACGGGGTCAT
TAGTTCATAG
CCCATATATG
GAGTTCCGCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAAT
GACGTATGTT
CCCATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGAGTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
CATATGCCAA
GTACGCCCCC
TATTGACGTC
AATGACGGTA
AATGGCCCGC
CTGGCATTAT
GCCCAGTACA
TGACCTTATG
GGACTTTCCT
ACTTGGCAGT
ACATCTACGT
ATTAGTCATC
GCTATTACCA
TGGTGATGCG
GTTTTGGCAG
TACATCAATG
GGCGTGGATA
GCGGTTTGAC
TCACGGGGAT
TTCCAAGTCT
CCACCCCATT
GACGTCAATG
GGAGTTTGTT
TTGGCACCAA
AATCAACGGG
ACTTTCCAAA
ATGTCGTAAC
AACTCCGCCC
CATTGACGCA
AATGGGCGGT
AGGCGTGTAC
GGTGGGAGGT
CTATATAAGC
AGAGCTGGTT
TAGTGAACCG
TCAGATCCGC
TAGCGCCACC
ATGGTGAGCA
AGGGCGAGGA
GGATAACATG
GCCATCATCA
AGGAGTTCAT
GCGCTTCAAG
GTGCACATGG
AGGGCTCCGT
GAACGGCCAC
GAGTTCGAGA
TCGAGGGCGA
GGGCGAGGGC
CGCCCCTACG
AGGGCACCCA
GACCGCCAAG
CTGAAGGTGA
CCAAGGGTGG
CCCCCTGCCC
TTCGCCTGGG
ACATCCTGTC
CCCTCAGTTC
ATGTACGGCT
CCAAGGCCTA
CGTGAAGCAC
CCCGCCGACA
TCCCCGACTA
CTTGAAGCTG
TCCTTCCCCG
AGGGCTTCAA
GTGGGAGCGC
GTGATGAACT
TCGATGACGG
CGGCGTGGTG
ACCGTGACCC
AGGACTCCTC
CCTGCAGGAC
GGCGAGTTCA
TCTACAAGGT
GAAGCTGCGC
GGCACCAACT
TCCCCTCCGA
CGGCCCCGTA
ATGCAGAAGA
AAACCATGGG
CTGGGAGGCC
TCCTCCGAGC
GGATGTACCC
CGAGGACGGC
GCCCTGAAGG
GCGAGATCAA
GCAGAGGCTG
AAGCTGAAGG
ACGGCGGCCA
CTACGACGCT
GAGGTCAAGA
CCACCTACAA
GGCCAAGAAG
CCCGTGCAGC
TGCCCGGCGC
CTACAACGTC
AACATCAAGT
TGGACATCAC
CTCCCACAAC
GAGGACTACA
CCATCGTGGA
ACAGTACGAA
CGCGCCGAGG
GCCGCCACTC
CACCGGCGGC
ATGGACGAGC
TGTACAAGTA
ACTCTTGGCC
CGGATCCCTG
TGCCTTCTAG
TTGCCAGCCA
TCTGTTGTTT
GCCCCTCCCC
CGTGCCTTCC
TTGACCCTGG
AAGGTGCCAC
TCCCACTGTC
CTTTCCTAAT
AAAATGAGGA
AATTGCATCG
CATTGTCTGA
GTAGGTGTCA
TTCTATTCTG
GGGGGTGGGG
TGGGGCAGGA
CAGCAAGGGG
GAGGATTGGG
AAAACAATAG
CAGGCATGCT
GGGGATGCGG
TGGGCTCTAT
GGGTCGACGG
CGCGCCCTAG
ACGAAGCAGC
TCCAGCCTAC
ACAATCGCTC
AAGACGTGTA
ATGCTTTTAT
TATATATTAG
TCACGATATC
TATAACAAGA
AAATATATAT
ATAATAAGTT
ATCACGTAAG
TAGAACATGA
AATAACAATA
TAATTATCGT
ATGAGTTAAA
TCTTAAAAGT
CACGTAAAAG
ATAATCATGC
GTCATTTTGA
CTCACGCGGT
CGTTATAGTT
CAAAATCAGT
GACACTTACC
GCATTGACAA
GCACGCCTCA
CGGGAGCTCC
AAGCGGCGAC
TGAGATGTCC
TAAATGCACA
GCGACGGATT
CGCGCTATTT
AGAAAGAGAG
AGCAATATTT
CAAGAATGCA
TGCGTCAATT
TTACGCAGAC
TATCTTTCTA
GGGTTAAGGA
TCCGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TATTGAAAAA
GGAAGAGTAT
GAGTATTCAA
CATTTCCGTG
TCGCCCTTAT
TCCCTTTTTT
GCGGCATTTT
GCCTTCCTGT
TTTTGCTCAC
CCAGAAACGC
TGGTGAAAGT
AAAAGATGCT
GAAGATCAGT
TGGGTGCACG
AGTGGGTTAC
ATCGAACTGG
ATCTCAACAG
CGGTAAGATC
CTTGAGAGTT
TTCGCCCCGA
AGAACGTTTT
CCAATGATGA
GCACTTTTAA
AGTTCTGCTA
TGTGGCGCGG
TATTATCCCG
TATTGACGCC
GGGCAAGAGC
AACTCGGTCG
CCGCATACAC
TATTCTCAGA
ATGACTTGGT
TGAGTACTCA
CCAGTCACAG
AAAAGCATCT
TACGGATGGC
ATGACAGTAA
GAGAATTATG
CAGTGCTGCC
ATAACCATGA
GTGATAACAC
TGCGGCCAAC
TTACTTCTGA
CAACGATCGG
AGGACCGAAG
GAGCTAACCG
CTTTTTTGCA
CAACATGGGG
GATCATGTAA
CTCGCCTTGA
TCGTTGGGAA
CCGGAGCTGA
ATGAAGCCAT
ACCAAACGAC
GAGCGTGACA
CCACGATGCC
TGTAGCAATG
GCAACAACGT
TGCGCAAACT
ATTAACTGGC
GAACTACTTA
CTCTAGCTTC
CCGGCAACAA
TTAATAGACT
GGATGGAGGC
GGATAAAGTT
GCAGGACCAC
TTCTGCGCTC
GGCCCTTCCG
GCTGGCTGGT
TTATTGCTGA
TAAATCTGGA
GCCGGTGAGC
GTGGGTCTCG
CGGTATCATT
GCAGCACTGG
GGCCAGATGG
TAAGCCCTCC
CGTATCGTAG
TTATCTACAC
GACGGGGAGT
CAGGCAACTA
TGGATGAACG
AAATAGACAG
ATCGCTGAGA
TAGGTGCCTC
ACTGATTAAG
CATTGGTAAC
TGTCAGACCA
AGTTTACTCA
TATATACTTT
AGATTGATTT
AAAACTTCAT
TTTTAATTTA
AAAGGATCTA
GGTGAAGATC
CTTTTTGATA
ATCTCATGAC
CAAAATCCCT
TAACGTGAGT
TTTCGTTCCA
CTGAGCGTCA
GACCCCGTAG
AAAAGATCAA
AGGATCTTCT
TGAGATCCTT
TTTTTCTGCG
CGTAATCTGC
TGCTTGCAAA
CAAAAAAACC
ACCGCTACCA
GCGGTGGTTT
GTTTGCCGGA
TCAAGAGCTA
CCAACTCTTT
TTCCGAAGGT
AACTGGCTTC
AGCAGAGCGC
AGATACCAAA
TACTGTTCTT
CTAGTGTAGC
CGTAGTTAGG
CCACCACTTC
AAGAACTCTG
TAGCACCGCC
TACATACCTC
GCTCTGCTAA
TCCTGTTACC
AGTGGCTGCT
GCCAGTGGCG
ATAAGTCGTG
TCTTACCGGG
TTGGACTCAA
GACGATAGTT
ACCGGATAAG
GCGCAGCGGT
CGGGCTGAAC
GGGGGGTTCG
TGCACACAGC
CCAGCTTGGA
GCGAACGACC
TACACCGAAC
TGAGATACCT
ACAGCGTGAG
CTATGAGAAA
GCGCCACGCT
TCCCGAAGGG
AGAAAGGCGG
ACAGGTATCC
GGTAAGCGGC
AGGGTCGGAA
CAGGAGAGCG
CACGAGGGAG
CTTCCAGGGG
GAAACGCCTG
GTATCTTTAT
AGTCCTGTCG
GGTTTCGCCA
CCTCTGACTT
GAGCGTCGAT
TTTTGTGATG
CTCGTCAGGG
GGGCGGAGCC
TATGGAAAAA
CGCCAGCAAC
GCGGCCTTTT
TACGGTTCCT
GGCCTTTTGC
TGGCCTTTTG
CTCACATGTT
CTTTCCTGCG
TTATCCCCTG
ATTCTCTCGA
GTTAACCCTA
GAAAGATAAT
CATATTGTGA
CGTACGTTAA
AGATAATCAT
GCGTAAAATT
GACGCATGTG
TTTTATCGAT
CTGTATATCG
AGGTTTATTT
ATTAATTTGA
ATAGATATTA
AGTTTTATTA
TATTTACACT
TACATACTAA
TAATAAATTC
AACAAACAAT
TTATTTATGT
TTATTTATTT
ATTAAAAAAA
AACAAAAACT
CAAAATTTCT
TCTATAAAGT
AACAAAACTT
TTAAACATTC
TCTCTTTTAC
AAAAATAAAC
TTATTTTGTA
CTTTAAAAAC
AGTCATGTTG
TATTATAAAA
TAAGTAATTA
GCTTAACTTA
TACATAATAG
AAACAAATTA
TACTTATTAA
TTAATCTAGA
GGTACCCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGAGTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
CATATGCCAA
GTACGCCCCC
TATTGACGTC
AATGACGGTA
AATGGCCCGC
CTGGCATTGT
GCCCAGTACA
TGACCTTATG
GGACTTTCCT
ACTTGGCAGT
ACATCTACGT
ATTAGTCATC
GCTATTACCA
TGGTCGAGGT
GAGCCCCACG
TTCTGCTTCA
CTCTCCCCAT
CTCCCCCCCC
TCCCCACCCC
CAATTTTGTA
TTTATTTATT
TTTTAATTAT
TTTGTGCAGC
GATGGGGGCG
GGGGGGGGGG