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CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

产品货号:P68695

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
别称: Plasmid#171380
基因长: 2898bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记:
荧光标记: 红色
启动子: CMV, U6
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体: pCX539
正向引物: U6-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CCGTGCCCCG

CTCCGCCGCC

GCCTCGCGCC

GCCCGCCCCG

GCTCTGACTG

ACCGCGTTAC

61

TCCCACAGGT

GAGCGGGCGG

GACGGCCCTT

CTCCTCCGGG

CTGTAATTAG

CTGAGCAAGA

121

GGTAAGGGTT

TAAGGGATGG

TTGGTTGGTG

GGGTATTAAT

GTTTAATTAC

CTGGAGCACC

181

TGCCTGAAAT

CACTTTTTTT

CAGGTTGGAC

CGGTGCCACC

ATGCCTAAAA

AGAAAAGAAA

241

GGTGGGTTCT

GGTATCGAGA

AGAAGAAGAG

CTTCGCCAAG

GGCATGGGAG

TGAAGAGCAC

301

CCTGGTGTCC

GGCTCTAAGG

TGTACATGAC

CACATTTGCT

GAGGGAAGCG

ACGCCAGGCT

361

GGAGAAGATC

GTGGAGGGCG

ATAGCATCAG

ATCCGTGAAC

GAGGGAGAGG

CTTTCAGCGC

421

CGAGATGGCT

GACAAGAACG

CTGGCTACAA

GATCGGAAAC

GCCAAGTTTT

CCCACCCAAA

481

GGGCTACGCC

GTGGTGGCTA

ACAACCCACT

GTACACCGGA

CCAGTGCAGC

AGGACATGCT

541

GGGACTGAAG

GAGACACTGG

AGAAGAGGTA

CTTCGGCGAG

TCCGCCGACG

GAAACGATAA

601

CATCTGCATC

CAGGTCATCC

ACAACATCCT

GGATATCGAG

AAGATCCTGG

CTGAGTACAT

661

CACAAACGCC

GCTTACGCCG

TGAACAACAT

CTCCGGCCTG

GACAAGGATA

TCATCGGCTT

721

CGGAAAGTTT

TCTACCGTGT

ACACATACGA

CGAGTTCAAG

GATCCAGAGC

ACCACCGGGC

781

CGCTTTTAAC

AACAACGACA

AGCTGATCAA

CGCCATCAAG

GCTCAGTACG

ACGAGTTCGA

841

TAACTTTCTG

GATAACCCCA

GGCTGGGCTA

CTTCGGACAG

GCTTTCTTTT

CTAAGGAGGG

901

CAGAAACTAC

ATCATCAACT

ACGGAAACGA

GTGTTACGAC

ATCCTGGCCC

TGCTGAGCGG

961

ACTGAGGCAC

TGGGTGGTGC

ACAACAACGA

GGAGGAGTCT

CGGATCAGCC

GCACCTGGCT

1021

GTACAACCTG

GACAAGAACC

TGGATAACGA

GTACATCTCC

ACACTGAACT

ACCTGTACGA

1081

CAGGATCACC

AACGAGCTGA

CAAACAGCTT

CTCCAAGAAC

TCTGCCGCTA

ACGTGAACTA

1141

CATCGCTGAG

ACCCTGGGCA

TCAACCCAGC

TGAGTTCGCT

GAGCAGTACT

TCAGATTTTC

1201

CATCATGAAG

GAGCAGAAGA

ACCTGGGCTT

CAACATCACA

AAGCTGAGAG

AAGTGATGCT

1261

GGACAGAAAG

GATATGTCCG

AGATCAGGAA

GAACCACAAG

GTGTTCGATT

CTATCAGAAC

1321

CAAGGTGTAC

ACAATGATGG

ACTTTGTGAT

CTACAGGTAC

TACATCGAGG

AGGATGCCAA

1381

GGTGGCCGCT

GCCAACAAGA

GCCTGCCCGA

CAACGAGAAG

TCTCTGAGCG

AGAAGGATAT

1441

CTTCGTGATC

AACCTGAGAG

GCTCCTTTAA

CGACGATCAG

AAGGACGCTC

TGTACTACGA

1501

TGAGGCCAAC

AGGATCTGGA

GAAAGCTGGA

GAACATCATG

CACAACATCA

AGGAGTTCCG

1561

GGGAAACAAG

ACCCGCGAGT

ACAAGAAGAA

GGACGCTCCA

AGGCTGCCTA

GGATCCTGCC

1621

TGCTGGAAGG

GACGTGAGCG

CCTTCAGCAA

GCTGATGTAC

GCCCTGACAA

TGTTTCTGGA

1681

CGGAAAGGAG

ATCAACGATC

TGCTGACCAC

ACTGATCAAC

AAGTTCGACA

ACATCCAGTC

1741

TTTTCTGAAA

GTGATGCCTC

TGATCGGCGT

GAACGCTAAG

TTCGTGGAGG

AGTACGCCTT

1801

CTTTAAGGAC

AGCGCCAAGA

TCGCTGATGA

GCTGCGGCTG

ATCAAGTCCT

TTGCCAGGAT

1861

GGGAGAGCCA

ATCGCTGACG

CTAGGAGAGC

TATGTACATC

GATGCCATCC

GGATCCTGGG

1921

AACCAACCTG

TCTTACGACG

AGCTGAAGGC

TCTGGCCGAC

ACCTTCAGCC

TGGATGAGAA

1981

CGGCAACAAG

CTGAAGAAGG

GCAAGCACGG

AATGCGCAAC

TTCATCATCA

ACAACGTGAT

2041

CAGCAACAAG

CGGTTTCACT

ACCTGATCAG

ATACGGCGAC

CCAGCTCACC

TGCACGAGAT

2101

CGCTAAGAAC

GAGGCCGTGG

TGAAGTTCGT

GCTGGGACGG

ATCGCCGATA

TCCAGAAGAA

2161

GCAGGGCCAG

AACGGAAAGA

ACCAGATCGA

CCGCTACTAC

GAGACCTGCA

TCGGCAAGGA

2221

TAAGGGAAAG

TCCGTGTCTG

AGAAGGTGGA

CGCTCTGACC

AAGATCATCA

CAGGCATGAA

2281

CTACGACCAG

TTCGATAAGA

AGAGATCTGT

GATCGAGGAC

ACCGGAAGGG

AGAACGCCGA

2341

GAGAGAGAAG

TTTAAGAAGA

TCATCAGCCT

GTACCTGACA

GTGATCTACC

ACATCCTGAA

2401

GAACATCGTG

AACATCAACG

CTAGATACGT

GATCGGCTTC

CACTGCGTGG

AGCGCGATGC

2461

CCAGCTGTAC

AAGGAGAAGG

GATACGACAT

CAACCTGAAG

AAGCTGGAGG

AGAAGGGCTT

2521

TAGCTCCGTG

ACCAAGCTGT

GCGCTGGAAT

CGACGAGACA

GCCCCCGACA

AGAGGAAGGA

2581

TGTGGAGAAG

GAGATGGCCG

AGAGAGCTAA

GGAGAGCATC

GACTCCCTGG

AGTCTGCTAA

2641

CCCTAAGCTG

TACGCCAACT

ACATCAAGTA

CTCCGATGAG

AAGAAGGCCG

AGGAGTTCAC

2701

CAGGCAGATC

AACAGAGAGA

AGGCCAAGAC

CGCTCTGAAC

GCCTACCTGA

GGAACACAAA

2761

GTGGAACGTG

ATCATCCGGG

AGGACCTGCT

GCGCATCGAT

AACAAGACCT

GTACACTGTT

2821

CCGGAACAAG

GCTGTGCACC

TGGAGGTGGC

TCGCTACGTG

CACGCCTACA

TCAACGACAT

2881

CGCCGAGGTG

AACTCCTACT

TTCAGCTGTA

CCACTACATC

ATGCAGAGGA

TCATCATGAA

2941

CGAGAGATAC

GAGAAGTCTA

GCGGCAAGGT

GTCTGAGTAC

TTCGACGCCG

TGAACGATGA

3001

GAAGAAGTAC

AACGATAGAC

TGCTGAAGCT

GCTGTGCGTG

CCTTTCGGAT

ACTGTATCCC

3061

ACGGTTTAAG

AACCTGAGCA

TCGAGGCCCT

GTTCGACCGC

AACGAGGCTG

CCAAGTTTGA

3121

TAAGGAGAAG

AAGAAGGTGA

GCGGCAACTC

CGGTTCTGGT

TACCCATACG

ACGTACCAGA

3181

TTACGCTCCC

AAGAAGAAGC

GCAAGGTGGG

ATAAAAGCTT

AACTTGTTTA

TTGCAGCTTA

3241

TAATGGTTAC

AAATAAAGCA

ATAGCATCAC

AAATTTCACA

AATAAAGCAT

TTTTTTCACT

3301

GCATTCTAGT

TGTGGTTTGT

CCAAACTCAT

CAATGTATCT

TATCATGTCT

GGATCACTAG

3361

TGAGGGCCTA

TTTCCCATGA

TTCCTTCATA

TTTGCATATA

CGATACAAGG

CTGTTAGAGA

3421

GATAATTGGA

ATTAATTTGA

CTGTAAACAC

AAAGATATTA

GTACAAAATA

CGTGACGTAG

3481

AAAGTAATAA

TTTCTTGGGT

AGTTTGCAGT

TTTAAAATTA

TGTTTTAAAA

TGGACTATCA

3541

TATGCTTACC

GTAACTTGAA

AGTATTTCGA

TTTCTTGGCT

TTATATATCT

TGTGGAAAGG

3601

ACGAAACACC

GCAAGTAAAC

CCCTACCAAC

TGGTCGGGGT

TTGAAACGGG

TCTTCGAGAA

3661

GACCTTTTTT

TTACGCGTTA

GTTATTAATA

GTAATCAATT

ACGGGGTCAT

TAGTTCATAG

3721

CCCATATATG

GAGTTCCGCG

TTACATAACT

TACGGTAAAT

GGCCCGCCTG

GCTGACCGCC

3781

CAACGACCCC

CGCCCATTGA

CGTCAATAAT

GACGTATGTT

CCCATAGTAA

CGCCAATAGG

3841

GACTTTCCAT

TGACGTCAAT

GGGTGGAGTA

TTTACGGTAA

ACTGCCCACT

TGGCAGTACA

3901

TCAAGTGTAT

CATATGCCAA

GTACGCCCCC

TATTGACGTC

AATGACGGTA

AATGGCCCGC

3961

CTGGCATTAT

GCCCAGTACA

TGACCTTATG

GGACTTTCCT

ACTTGGCAGT

ACATCTACGT

4021

ATTAGTCATC

GCTATTACCA

TGGTGATGCG

GTTTTGGCAG

TACATCAATG

GGCGTGGATA

4081

GCGGTTTGAC

TCACGGGGAT

TTCCAAGTCT

CCACCCCATT

GACGTCAATG

GGAGTTTGTT

4141

TTGGCACCAA

AATCAACGGG

ACTTTCCAAA

ATGTCGTAAC

AACTCCGCCC

CATTGACGCA

4201

AATGGGCGGT

AGGCGTGTAC

GGTGGGAGGT

CTATATAAGC

AGAGCTGGTT

TAGTGAACCG

4261

TCAGATCCGC

TAGCGCCACC

ATGGTGAGCA

AGGGCGAGGA

GGATAACATG

GCCATCATCA

4321

AGGAGTTCAT

GCGCTTCAAG

GTGCACATGG

AGGGCTCCGT

GAACGGCCAC

GAGTTCGAGA

4381

TCGAGGGCGA

GGGCGAGGGC

CGCCCCTACG

AGGGCACCCA

GACCGCCAAG

CTGAAGGTGA

4441

CCAAGGGTGG

CCCCCTGCCC

TTCGCCTGGG

ACATCCTGTC

CCCTCAGTTC

ATGTACGGCT

4501

CCAAGGCCTA

CGTGAAGCAC

CCCGCCGACA

TCCCCGACTA

CTTGAAGCTG

TCCTTCCCCG

4561

AGGGCTTCAA

GTGGGAGCGC

GTGATGAACT

TCGATGACGG

CGGCGTGGTG

ACCGTGACCC

4621

AGGACTCCTC

CCTGCAGGAC

GGCGAGTTCA

TCTACAAGGT

GAAGCTGCGC

GGCACCAACT

4681

TCCCCTCCGA

CGGCCCCGTA

ATGCAGAAGA

AAACCATGGG

CTGGGAGGCC

TCCTCCGAGC

4741

GGATGTACCC

CGAGGACGGC

GCCCTGAAGG

GCGAGATCAA

GCAGAGGCTG

AAGCTGAAGG

4801

ACGGCGGCCA

CTACGACGCT

GAGGTCAAGA

CCACCTACAA

GGCCAAGAAG

CCCGTGCAGC

4861

TGCCCGGCGC

CTACAACGTC

AACATCAAGT

TGGACATCAC

CTCCCACAAC

GAGGACTACA

4921

CCATCGTGGA

ACAGTACGAA

CGCGCCGAGG

GCCGCCACTC

CACCGGCGGC

ATGGACGAGC

4981

TGTACAAGTA

ACTCTTGGCC

CGGATCCCTG

TGCCTTCTAG

TTGCCAGCCA

TCTGTTGTTT

5041

GCCCCTCCCC

CGTGCCTTCC

TTGACCCTGG

AAGGTGCCAC

TCCCACTGTC

CTTTCCTAAT

5101

AAAATGAGGA

AATTGCATCG

CATTGTCTGA

GTAGGTGTCA

TTCTATTCTG

GGGGGTGGGG

5161

TGGGGCAGGA

CAGCAAGGGG

GAGGATTGGG

AAAACAATAG

CAGGCATGCT

GGGGATGCGG

5221

TGGGCTCTAT

GGGTCGACGG

CGCGCCCTAG

ACGAAGCAGC

TCCAGCCTAC

ACAATCGCTC

5281

AAGACGTGTA

ATGCTTTTAT

TATATATTAG

TCACGATATC

TATAACAAGA

AAATATATAT

5341

ATAATAAGTT

ATCACGTAAG

TAGAACATGA

AATAACAATA

TAATTATCGT

ATGAGTTAAA

5401

TCTTAAAAGT

CACGTAAAAG

ATAATCATGC

GTCATTTTGA

CTCACGCGGT

CGTTATAGTT

5461

CAAAATCAGT

GACACTTACC

GCATTGACAA

GCACGCCTCA

CGGGAGCTCC

AAGCGGCGAC

5521

TGAGATGTCC

TAAATGCACA

GCGACGGATT

CGCGCTATTT

AGAAAGAGAG

AGCAATATTT

5581

CAAGAATGCA

TGCGTCAATT

TTACGCAGAC

TATCTTTCTA

GGGTTAAGGA

TCCGCACTTT

5641

TCGGGGAAAT

GTGCGCGGAA

CCCCTATTTG

TTTATTTTTC

TAAATACATT

CAAATATGTA

5701

TCCGCTCATG

AGACAATAAC

CCTGATAAAT

GCTTCAATAA

TATTGAAAAA

GGAAGAGTAT

5761

GAGTATTCAA

CATTTCCGTG

TCGCCCTTAT

TCCCTTTTTT

GCGGCATTTT

GCCTTCCTGT

5821

TTTTGCTCAC

CCAGAAACGC

TGGTGAAAGT

AAAAGATGCT

GAAGATCAGT

TGGGTGCACG

5881

AGTGGGTTAC

ATCGAACTGG

ATCTCAACAG

CGGTAAGATC

CTTGAGAGTT

TTCGCCCCGA

5941

AGAACGTTTT

CCAATGATGA

GCACTTTTAA

AGTTCTGCTA

TGTGGCGCGG

TATTATCCCG

6001

TATTGACGCC

GGGCAAGAGC

AACTCGGTCG

CCGCATACAC

TATTCTCAGA

ATGACTTGGT

6061

TGAGTACTCA

CCAGTCACAG

AAAAGCATCT

TACGGATGGC

ATGACAGTAA

GAGAATTATG

6121

CAGTGCTGCC

ATAACCATGA

GTGATAACAC

TGCGGCCAAC

TTACTTCTGA

CAACGATCGG

6181

AGGACCGAAG

GAGCTAACCG

CTTTTTTGCA

CAACATGGGG

GATCATGTAA

CTCGCCTTGA

6241

TCGTTGGGAA

CCGGAGCTGA

ATGAAGCCAT

ACCAAACGAC

GAGCGTGACA

CCACGATGCC

6301

TGTAGCAATG

GCAACAACGT

TGCGCAAACT

ATTAACTGGC

GAACTACTTA

CTCTAGCTTC

6361

CCGGCAACAA

TTAATAGACT

GGATGGAGGC

GGATAAAGTT

GCAGGACCAC

TTCTGCGCTC

6421

GGCCCTTCCG

GCTGGCTGGT

TTATTGCTGA

TAAATCTGGA

GCCGGTGAGC

GTGGGTCTCG

6481

CGGTATCATT

GCAGCACTGG

GGCCAGATGG

TAAGCCCTCC

CGTATCGTAG

TTATCTACAC

6541

GACGGGGAGT

CAGGCAACTA

TGGATGAACG

AAATAGACAG

ATCGCTGAGA

TAGGTGCCTC

6601

ACTGATTAAG

CATTGGTAAC

TGTCAGACCA

AGTTTACTCA

TATATACTTT

AGATTGATTT

6661

AAAACTTCAT

TTTTAATTTA

AAAGGATCTA

GGTGAAGATC

CTTTTTGATA

ATCTCATGAC

6721

CAAAATCCCT

TAACGTGAGT

TTTCGTTCCA

CTGAGCGTCA

GACCCCGTAG

AAAAGATCAA

6781

AGGATCTTCT

TGAGATCCTT

TTTTTCTGCG

CGTAATCTGC

TGCTTGCAAA

CAAAAAAACC

6841

ACCGCTACCA

GCGGTGGTTT

GTTTGCCGGA

TCAAGAGCTA

CCAACTCTTT

TTCCGAAGGT

6901

AACTGGCTTC

AGCAGAGCGC

AGATACCAAA

TACTGTTCTT

CTAGTGTAGC

CGTAGTTAGG

6961

CCACCACTTC

AAGAACTCTG

TAGCACCGCC

TACATACCTC

GCTCTGCTAA

TCCTGTTACC

7021

AGTGGCTGCT

GCCAGTGGCG

ATAAGTCGTG

TCTTACCGGG

TTGGACTCAA

GACGATAGTT

7081

ACCGGATAAG

GCGCAGCGGT

CGGGCTGAAC

GGGGGGTTCG

TGCACACAGC

CCAGCTTGGA

7141

GCGAACGACC

TACACCGAAC

TGAGATACCT

ACAGCGTGAG

CTATGAGAAA

GCGCCACGCT

7201

TCCCGAAGGG

AGAAAGGCGG

ACAGGTATCC

GGTAAGCGGC

AGGGTCGGAA

CAGGAGAGCG

7261

CACGAGGGAG

CTTCCAGGGG

GAAACGCCTG

GTATCTTTAT

AGTCCTGTCG

GGTTTCGCCA

7321

CCTCTGACTT

GAGCGTCGAT

TTTTGTGATG

CTCGTCAGGG

GGGCGGAGCC

TATGGAAAAA

7381

CGCCAGCAAC

GCGGCCTTTT

TACGGTTCCT

GGCCTTTTGC

TGGCCTTTTG

CTCACATGTT

7441

CTTTCCTGCG

TTATCCCCTG

ATTCTCTCGA

GTTAACCCTA

GAAAGATAAT

CATATTGTGA

7501

CGTACGTTAA

AGATAATCAT

GCGTAAAATT

GACGCATGTG

TTTTATCGAT

CTGTATATCG

7561

AGGTTTATTT

ATTAATTTGA

ATAGATATTA

AGTTTTATTA

TATTTACACT

TACATACTAA

7621

TAATAAATTC

AACAAACAAT

TTATTTATGT

TTATTTATTT

ATTAAAAAAA

AACAAAAACT

7681

CAAAATTTCT

TCTATAAAGT

AACAAAACTT

TTAAACATTC

TCTCTTTTAC

AAAAATAAAC

7741

TTATTTTGTA

CTTTAAAAAC

AGTCATGTTG

TATTATAAAA

TAAGTAATTA

GCTTAACTTA

7801

TACATAATAG

AAACAAATTA

TACTTATTAA

TTAATCTAGA

GGTACCCGTT

ACATAACTTA

7861

CGGTAAATGG

CCCGCCTGGC

TGACCGCCCA

ACGACCCCCG

CCCATTGACG

TCAATAGTAA

7921

CGCCAATAGG

GACTTTCCAT

TGACGTCAAT

GGGTGGAGTA

TTTACGGTAA

ACTGCCCACT

7981

TGGCAGTACA

TCAAGTGTAT

CATATGCCAA

GTACGCCCCC

TATTGACGTC

AATGACGGTA

8041

AATGGCCCGC

CTGGCATTGT

GCCCAGTACA

TGACCTTATG

GGACTTTCCT

ACTTGGCAGT

8101

ACATCTACGT

ATTAGTCATC

GCTATTACCA

TGGTCGAGGT

GAGCCCCACG

TTCTGCTTCA

8161

CTCTCCCCAT

CTCCCCCCCC

TCCCCACCCC

CAATTTTGTA

TTTATTTATT

TTTTAATTAT

8221

TTTGTGCAGC

GATGGGGGCG

GGGGGGGGGG

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