产品货号：P68577
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV3-CMV-Aff4(mouse)-3×FLAG-CopGFP-Puro |
|---|---|
| 别称: | NM_033565.2;AF5Q31; Alf4; Laf4l; MCEF; |
| 基因长: | 3480bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 小鼠 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 绿色 |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGA
ACCGTGAAGA
CCGGAATGTG
CTGCGCATGA
AAGAAAGGGA
AAGGCGGAAT
CAGGAAATTC
AGCAGGGTGA
AGACGCCTTC
CCACCTAGTT
CTCCTCTCTT
TGCTGAGCCA
TACAAAGTCA
CTAGCAAAGA
AGATAAGTTA
TCAAGTCGGA
TTCAGAGCAT
GCTTGGAAAC
TACGATGAAA
TGAAGGATTA
CATAGGAGAC
AGATCGATAC
CAAAGCTTGT
TGCAATTCCC
AAGCCTGCAG
TACCAACGAC
CACAGACGAG
AAAGCAAACC
CAAATTTTTT
TGAGCAGAGA
CATGGAGGAT
CTCATCAGAG
TAGCAAATGG
ACTCCTGTAG
GCCCCGCCCC
CAGCACCTCT
CAGTCTCAGA
AACGCTCCTC
TGCCCTGCAG
AGTGGACACA
GCAGCCAACG
GAGTGGTGCA
GGAGGGAGTG
GCGCCAGCAG
CAGTGGCCAG
AGGCATGACC
GTGACTCCTA
CAGCAGTAGC
AGGAAAAAAG
GCCAGCACGG
CTCAGAGCAC
TCCAAATCTC
GATCCTCAAG
CCCTGGAAAA
CCCCAGGCTG
TTTCTTCATT
AAGTTCTAGT
CATTCCAGGT
CTCATGGGAA
TGATCACCAT
AGCAAGGAAC
ATCAGCGCTC
CAAATCACCT
CGGGACCCTG
ATGCCAACTG
GGATTCTCCT
TCCCGCGGAC
CTTTTTCAAG
TGGGCAACAT
TCAAGTCAGT
CTTTCCCACC
TTCTTTGATG
TCAAAATCTA
GTTCAATGTT
ACAGAAACCC
ACTGCCTATG
TACGGCCCAT
GGATGGACAG
GAGTCTGTGG
AACCAAAGCT
GTCCTCTGAG
CACTACAGCA
GCCAGTCTCA
TGGTAATAGC
ATGACTGAGC
TGAAGCCCAG
CAGCAAAGCA
CATCTCACCA
AGCTGAAAAT
TCCTTCCCGA
CCTTTGGATG
CATCGGTTTC
TGGTGATGTA
AGCTGTGTAG
ATGAAATACT
TAAAGAGATG
ACGCATTCAT
GGCCTCCCCC
TCTAACGGCT
ATTCATACAC
CATGCAAAAC
AGAACCTTCC
AAATTCCCTT
TTCCAACTAA
GGAGTCGCAA
CAGTCTAACT
TTGGCCCTGG
AGAACAAAAA
AGATACAGTA
CTGCTAAAAC
ATCAAATGGA
CACCAGTCAA
AATCTATGTT
AAAAGATGAC
TTAAAACTAA
GCAGCAGTGA
AGACAGTGAT
GGGGAACAGG
ATTGTGATAA
GACAATGCCA
AGGAGTACAC
CAGGAAGTAA
CTCTGAACCT
TCACACCATA
ACAGTGAGGG
AGCAGACAAC
TCCAGGGATG
ACTCGAGCAG
CCACAGTGGA
TCTGAGAGCA
GCTCTGGGTC
TGACTCGGAG
AGTGAGAGCA
GCTCCAGTGA
CAGTGAGGCT
AATGAGCCGT
CGCAGAGCGC
ATCTCCTGAG
CCTGAACCAC
CACCAACAAA
CAAGTGGCAA
CTTGATAATT
GGTTGAATAA
AGTGAACCCA
CATAAAGTAT
CCCCAGCTTC
CTCTGTAGAC
AGCAACATCC
CATCTTCTCA
AGCCTACAAG
AAAGAAGGCC
GGGAGCAGGG
CACTGCCAGT
AACTACACAG
ATCCAGGGGG
GACAAAAGAA
ACGAGCTCTG
CCACCCCAGG
ACGAGACTCT
AAAACCATCC
AAAAGGGATC
AGAAAGTGGC
CGTGGGAGGC
AGAAGTCTCC
TGCACAGAGC
GACAGTACCA
CTCAGAGGAG
AACTGTCGGC
AAAAAACAAC
CCAAAAAACC
TGAGAAGTCA
GCTGCTGAAG
AGCCTCGTGG
AGGCCTGAAG
ATAGAAAGTG
AGACCCCTGT
GGACATGGCT
GCCAGCATGC
CCTCCAGCAG
GCACAAAGCA
GCCACCAAGG
GCTCGAGGAA
ACCCAACATC
AAAAAGGAAT
CAAAATCTTC
TCCTCGACCT
ACGGCAGAGA
AGAAGAAGTA
CAAGTCAGCC
AGTAAACCGT
CCCAGAAGTC
TCGGGAGATC
ATAGAGACTG
ACACCTCGTC
CTCAGACTCG
GACGGCAGCG
AGAGCCTGCC
TCCCTCCTCA
CAGACTCCTA
AGTACCCTGA
AAGCAACAGG
ACTCCTGTCA
AACCCTCCTC
AGTGGAGGAA
GAAGATAGCT
TTTTTCGGCA
ACGAATGTTC
TCTCCTATGG
AAGAGAAGGA
GCTCCTTTCC
CCACTCAGTG
AGCCTGATGA
CAGGTATCCA
CTCATTGTGA
AGATTGACCT
CAATCTTCTG
ACTCGAATAC
CCGGGAAGCC
TTACAAAGAA
ACAGAGCCAC
CCAAGGGGGA
AAAGAAAAAT
GTTCCAGAGA
AACACTCGAG
AGAGGTGCAG
AAGCAAGCCT
CAGAAAAGGC
TTCCAACAAA
GGCAAGAGGA
AACACAAGAA
TGATGATGAT
ACCCGAGCCA
GTGAGAGCAA
AAAACCCAAA
ACAGAGGACA
AGAACTCATC
AGGCCACAAG
CCATCCAGCA
GCAGAGAGTC
ATCTAAGCAG
AGTAGTACAA
AAGAAAAGGA
CTTGCTACCT
TCACCTGCTG
GGCCCATTCT
TTCAAAAGAT
TCAAAAACAG
AGCATGGTTC
TCGGAAGAGG
ACTGTCAGTC
AGTCGTCGTC
CTTAAAGTCC
AGTGGGACCA
GCAGCAAAGA
GAACAGTGGC
AGCAGCAGCA
AGAGCAGCTC
CTCCTCCACA
GCGAAGCAGA
AGAAGACTGA
AGGCAAGGGT
CCCAGCAGCT
CCAAGGAGGC
CAAGGAGAAG
GCTCCAAATA
GCTCCTCTAA
TTGTCCTCCG
TCTACACCAA
CTTCTGAGTC
TTCGAAGCCT
CGGAGAACAA
AGCTTGCCTT
TGATGACAGA
AATTATTCAG
CAGACCATTA
TTTACAAGAA
GCAAAAAAAC
TCAAACACAA
TGCAGATGCT
TTGTCTGACA
GGTTTGAGAA
AGCCGTGTAC
TACCTTGATG
CTGTAGTGTC
TTTCATTGAG
TGTGGGAATG
CATTAGAGAA
GAACGCTCAG
GAATCCAAGT
CCCCATTCCC
TATGTACTCA
GACACGGTGG
AGCTCATTAA
GTACACTATG
AAACTAAAGA
ATTATTTGGC
ACCAGATGCT
ACAGCTGCAG
ACAAGAGACT
CACAGTACTT
TGCCTGAGAT
GCCAGTCATT
ATTGTACCTG
AGGCTGTTCA
AACTGAAGAA
GGAGAACGCT
CTCAAGTACT
CCAAGACGCT
AACTGAGCAC
CTGAAGAATT
CTTACAGTAA
TTCTCAAGCA
CCTTCACCCG
GCTTGGGGAG
CAAAGCTGTT
GGGATGCCCT
CCCCTGTTTC
TCCAAAACTG
TCACCAGGCA
ATTCTGGAAG
TTACTCTTCT
GGGGGAAGTA
GTGCTTCAGC
AAGTGGTTCT
TCAGTGACCA
TTCCACAGAA
AATCCACCAG
ATGGCAGCAA
GCTATGTCCA
GGTTACATCT
AACTTCCTCT
ATGCCACAGA
AATTTGGGAC
CAAGCTGAAC
AGCTTTCCAA
AGAACAAAAA
GAATTCTTTG
CTGAACTGGA
CAAAGTGATG
GGCCCTCTCA
TCTTTAATGC
AAGCATCATG
ACAGATCTGG
CTCGTTACAC
CCGGCAGGGA
CTGCATTGGC
TTCGGCAGGA
TGCCAAGCTG
ATATCTGGAT
CCGACTACAA
AGACCATGAC
GGTGATTATA
AAGATCATGA
CATCGACTAC
AAGGATGACG
ATGACAAGTA
GCGGCCGCGA
AGGATCTGCG
ATCGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACGGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGCTGAAGC
TTCGAGGGGC
TCGCATCTCT
CCTTCACGCG
CCCGCCGCCC
TACCTGAGGC
CGCCATCCAC
GCCGGTTGAG
TCGCGTTCTG
CCGCCTCCCG
CCTGTGGTGC
CTCCTGAACT
GCGTCCGCCG
TCTAGGTAAG
TTTAAAGCTC
AGGTCGAGAC
CGGGCCTTTG
TCCGGCGCTC
CCTTGGAGCC
TACCTAGACT
CAGCCGGCTC
TCCACGCTTT
GCCTGACCCT
GCTTGCTCAA
CTCTACGTCT
TTGTTTCGTT
TTCTGTTCTG
CGCCGTTACA
GATCCAAGCT
GTGACCGGCG
CCTACGCTAG
ACGCCACCAT
GGAGAGCGAC
GAGAGCGGCC
TGCCCGCCAT
GGAGATCGAG
TGCCGCATCA
CCGGCACCCT
GAACGGCGTG
GAGTTCGAGC
TGGTGGGCGG
CGGAGAGGGC
ACCCCCAAGC
AGGGCCGCAT
GACCAACAAG
ATGAAGAGCA
CCAAAGGCGC
CCTGACCTTC
AGCCCCTACC
TGCTGAGCCA
CGTGATGGGC
TACGGCTTCT
ACCACTTCGG
CACCTACCCC
AGCGGCTACG
AGAACCCCTT
CCTGCACGCC
ATCAACAACG
GCGGCTACAC
CAACACCCGC
ATCGAGAAGT
ACGAGGACGG
CGGCGTGCTG
CACGTGAGCT
TCAGCTACCG
CTACGAGGCC
GGCCGCGTGA
TCGGCGACTT
CAAGGTGGTG
GGCACCGGCT
TCCCCGAGGA
CAGCGTGATC
TTCACCGACA
AGATCATCCG
CAGCAACGCC
ACCGTGGAGC
ACCTGCACCC
CATGGGCGAT
AACGTGCTGG
TGGGCAGCTT
CGCCCGCACC
TTCAGCCTGC
GCGACGGCGG
CTACTACAGC
TTCGTGGTGG
ACAGCCACAT
GCACTTCAAG
AGCGCCATCC
ACCCCAGCAT
CCTGCAGAAC
GGGGGCCCCA
TGTTCGCCTT
CCGCCGCGTG
GAGGAGCTGC
ACAGCAACAC
CGAGCTGGGC
ATCGTGGAGT
ACCAGCACGC
CTTCAAGACC
CCCATCGCCT
TCGCCAGATC
CCGCGCTCAG
TCGTCCAATT
CTGCCGTGGA
CGGCACCGCC
GGACCCGGCT
CCACCGGATC
TCGCGAGGGC
AGAGGAAGTC
TTCTAACATG
CGGTGACGTG
GAGGAGAATC
CCGGCCCTAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAATCATCG
TCCTTTCCTT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCCTG
GTACCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAT
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTAGTA
GTTCATGTCA
TCTTATTATT
CAGTATTTAT
AACTTGCAAA
GAAATGAATA
TCAGAGAGTG
AGAGGAACTT
GTTTATTGCA
GCTTATAATG
GTTACAAATA
AAGCAATAGC
ATCACAAATT
TCACAAATAA
AGCATTTTTT
TCACTGCATT
CTAGTTGTGG
TTTGTCCAAA
CTCATCAATG
TATCTTATCA
TGTCTGGCTC
TAGCTATCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGACTTT
TGCAGAGACC
AAATTCGTAA
TCATGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTCGC
GCGTTTCGGT
GATGACGGTG
AAAACCTCTG
ACACATGCAG
CTCCCGGAGA
CGGTCACAGC
TTGTCTGTAA
GCGGATGCCG
GGAGCAGACA
AGCCCGTCAG
GGCGCGTCAG
CGGGTGTTGG
CGGGTGTCGG
GGCTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCCATTC
GCCATTCAGG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGCCAAGCTG
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