名称: | Fuw-dCas9-Tet1CD_IM |
---|---|
别称: | Plasmid #84479 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Bleo |
荧光标记: | |
启动子: | UbC |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | FUW |
正向引物: | |
反向引物: | WPRE-R |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGCTACAACA
AGGCAAGGCT
TGACCGACAA
TTGCATGAAG
AATCTGCTTA
GGGTTAGGCG
TTTTGCGCTG
CTTCGCGATG
TACGGGCCAG
ATATACGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GCGCGTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGCTGATC
TTCAGACCTG
GAGGAGGAGA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCGGCACT
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGTTA
ATTAACCCGT
GTCGGCTCCA
GATCTGGCCT
CCGCGCCGGG
TTTTGGCGCC
TCCCGCGGGC
GCCCCCCTCC
TCACGGCGAG
CGCTGCCACG
TCAGACGAAG
GGCGCAGCGA
GCGTCCTGAT
CCTTCCGCCC
GGACGCTCAG
GACAGCGGCC
CGCTGCTCAT
AAGACTCGGC
CTTAGAACCC
CAGTATCAGC
AGAAGGACAT
TTTAGGACGG
GACTTGGGTG
ACTCTAGGGC
ACTGGTTTTC
TTTCCAGAGA
GCGGAACAGG
CGAGGAAAAG
TAGTCCCTTC
TCGGCGATTC
TGCGGAGGGA
TCTCCGTGGG
GCGGTGAACG
CCGATGATTA
TATAAGGACG
CGCCGGGTGT
GGCACAGCTA
GTTCCGTCGC
AGCCGGGATT
TGGGTCGCGG
TTCTTGTTTG
TGGATCGCTG
TGATCGTCAC
TTGGTGAGTA
GCGGGCTGCT
GGGCTGGCCG
GGGCTTTCGT
GGCCGCCGGG
CCGCTCGGTG
GGACGGAAGC
GTGTGGAGAG
ACCGCCAAGG
GCTGTAGTCT
GGGTCCGCGA
GCAAGGTTGC
CCTGAACTGG
GGGTTGGGGG
GAGCGCAGCA
AAATGGCGGC
TGTTCCCGAG
TCTTGAATGG
AAGACGCTTG
TGAGGCGGGC
TGTGAGGTCG
TTGAAACAAG
GTGGGGGGCA
TGGTGGGCGG
CAAGAACCCA
AGGTCTTGAG
GCCTTCGCTA
ATGCGGGAAA
GCTCTTATTC
GGGTGAGATG
GGCTGGGGCA
CCATCTGGGG
ACCCTGACGT
GAAGTTTGTC
ACTGACTGGA
GAACTCGGTT
TGTCGTCTGT
TGCGGGGGCG
GCAGTTATGG
CGGTGCCGTT
GGGCAGTGCA
CCCGTACCTT
TGGGAGCGCG
CGCCCTCGTC
GTGTCGTGAC
GTCACCCGTT
CTGTTGGCTT
ATAATGCAGG
GTGGGGCCAC
CTGCCGGTAG
GTGTGCGGTA
GGCTTTTCTC
CGTCGCAGGA
CGCAGGGTTC
GGGCCTAGGG
TAGGCTCTCC
TGAATCGACA
GGCGCCGGAC
CTCTGGTGAG
GGGAGGGATA
AGTGAGGCGT
CAGTTTCTTT
GGTCGGTTTT
ATGTACCTAT
CTTCTTAAGT
AGCTGAAGCT
CCGGTTTTGA
ACTATGCGCT
CGGGGTTGGC
GAGTGTGTTT
TGTGAAGTTT
TTTAGGCACC
TTTTGAAATG
TAATCATTTG
GGTCAATATG
TAATTTTCAG
TGTTAGACTA
GTAAATTGTC
CGCTAAATTC
TGGCCGTTTT
TGGCTTTTTT
GTTAGACGAA
GCTTGGGCTG
CAGGTCGACT
CTAGAAGTTA
ACCTCGAGGG
CGCGCCCATG
GACAAGAAGT
ATTCTATCGG
ACTGGCCATC
GGGACTAATA
GCGTCGGGTG
GGCCGTGATC
ACTGACGAGT
ACAAGGTGCC
CTCTAAGAAG
TTCAAGGTGC
TCGGGAACAC
CGACCGGCAT
TCCATCAAGA
AAAATCTGAT
CGGAGCTCTC
CTCTTTGATT
CAGGGGAGAC
CGCTGAAGCA
ACCCGCCTCA
AGCGGACTGC
TAGACGGCGG
TACACCAGGA
GGAAGAACCG
GATTTGTTAC
CTTCAAGAGA
TATTCTCCAA
CGAAATGGCA
AAGGTCGACG
ACAGCTTCTT
CCATAGGCTG
GAAGAATCAT
TCCTCGTGGA
AGAGGATAAG
AAGCATGAAC
GGCATCCCAT
CTTCGGTAAT
ATCGTCGACG
AGGTGGCCTA
TCACGAGAAA
TACCCAACCA
TCTACCATCT
TCGCAAAAAG
CTGGTGGACT
CAACCGACAA
GGCAGACCTC
CGGCTTATCT
ACCTGGCCCT
GGCCCACATG
ATCAAGTTCA
GAGGCCACTT
CCTGATCGAG
GGCGACCTCA
ATCCTGACAA
TAGCGATGTG
GATAAACTGT
TCATCCAGCT
GGTGCAGACT
TACAACCAGC
TCTTTGAAGA
GAACCCCATC
AATGCAAGCG
GAGTCGATGC
CAAGGCCATT
CTGTCAGCCC
GGCTGTCAAA
GAGCCGCAGA
CTTGAGAATC
TTATCGCTCA
GCTGCCGGGT
GAAAAGAAAA
ATGGACTGTT
CGGGAACCTG
ATTGCTCTTT
CACTTGGGCT
GACTCCCAAT
TTCAAGTCTA
ATTTCGACCT
GGCAGAGGAT
GCCAAGCTGC
AACTGTCCAA
GGACACCTAT
GATGACGATC
TCGACAACCT
CCTGGCCCAG
ATCGGTGACC
AATACGCCGA
CCTTTTCCTT
GCTGCTAAGA
ATCTTTCTGA
CGCCATCCTG
CTGTCTGACA
TTCTCCGCGT
GAACACTGAA
ATCACCAAGG
CCCCTCTTTC
AGCTTCAATG
ATTAAGCGGT
ATGATGAGCA
CCACCAGGAC
CTGACCCTGC
TTAAGGCACT
CGTCCGGCAG
CAGCTTCCGG
AGAAGTACAA
GGAAATCTTC
TTTGACCAGT
CAAAGAATGG
ATACGCCGGC
TACATCGACG
GAGGTGCCTC
CCAAGAGGAA
TTTTATAAGT
TTATCAAACC
TATCCTTGAG
AAGATGGACG
GCACCGAAGA
GCTCCTCGTG
AAACTGAATC
GGGAGGATCT
GCTGCGGAAG
CAGCGCACTT
TCGACAATGG
GAGCATTCCC
CACCAGATCC
ATCTTGGGGA
GCTTCACGCC
ATCCTTCGGC
GCCAAGAGGA
CTTCTACCCC
TTTCTTAAGG
ACAACAGGGA
GAAGATTGAG
AAAATTCTCA
CTTTCCGCAT
CCCCTACTAC
GTGGGACCCC
TCGCCAGAGG
AAATAGCCGG
TTTGCTTGGA
TGACCAGAAA
GTCAGAAGAA
ACTATCACTC
CCTGGAACTT
CGAAGAGGTG
GTGGACAAGG
GAGCCAGCGC
TCAGTCATTC
ATCGAACGGA
TGACTAACTT
CGATAAGAAC
CTCCCCAATG
AGAAGGTCCT
GCCGAAACAT
TCCCTGCTCT
ACGAGTACTT
TACCGTGTAC
AACGAGCTGA
CCAAGGTGAA
ATATGTCACC
GAAGGGATGA
GGAAGCCCGC
ATTCCTGTCA
GGCGAACAAA
AGAAGGCAAT
TGTGGACCTT
CTGTTCAAGA
CCAATAGAAA
GGTGACCGTG
AAGCAGCTGA
AGGAGGACTA
TTTCAAGAAA
ATTGAATGCT
TCGACTCTGT
GGAGATTAGC
GGGGTCGAAG
ATCGGTTCAA
CGCAAGCCTG
GGTACCTACC
ATGATCTGCT
TAAGATCATC
AAGGACAAGG
ATTTTCTGGA
CAATGAGGAG
AACGAGGACA
TCCTTGAGGA
CATTGTCCTG
ACTCTCACTC
TGTTCGAGGA
CCGGGAAATG
ATCGAGGAGA
GGCTTAAGAC
CTACGCCCAT
CTGTTCGACG
ATAAAGTGAT
GAAGCAACTT
AAACGGAGAA
GATATACCGG
ATGGGGACGC
CTTAGCCGCA
AACTCATCAA
CGGAATCCGG
GACAAACAGA
GCGGAAAGAC
CATTCTTGAT
TTCCTTAAGA
GCGACGGATT
CGCTAATCGC
AACTTCATGC
AACTTATCCA
TGATGATTCC
CTGACCTTTA
AGGAGGACAT
CCAGAAGGCC
CAAGTGTCTG
GACAAGGTGA
CTCACTGCAC
GAGCATATCG
CAAATCTGGC
TGGTTCACCC
GCTATTAAGA
AGGGTATTCT
CCAGACCGTG
AAAGTCGTGG
ACGAGCTGGT
CAAGGTGATG
GGTCGCCATA
AACCAGAGAA
CATTGTCATC
GAGATGGCCA
GGGAAAACCA
GACTACCCAG
AAGGGACAGA
AGAACAGCAG
GGAGCGGATG
AAAAGAATTG
AGGAAGGGAT
TAAGGAGCTC
GGGTCACAGA
TCCTTAAAGA
GCACCCGGTG
GAAAACACCC
AGCTTCAGAA
TGAGAAGCTC
TATCTGTACT
ACCTTCAAAA
TGGACGCGAT
ATGTATGTGG
ACCAAGAGCT
TGATATCAAC
AGGCTCTCAG
ACTACGACGT
GGACGCCATC
GTCCCTCAGA
GCTTCCTCAA
AGACGACTCA
ATTGACAATA
AGGTGCTGAC
TCGCTCAGAC
AAGAACCGGG
GAAAGTCAGA
TAACGTGCCC
TCAGAGGAAG
TCGTGAAAAA
GATGAAGAAC
TATTGGCGCC
AGCTTCTGAA
CGCAAAGCTG
ATCACTCAGC
GGAAGTTCGA
CAATCTCACT
AAGGCTGAGA
GGGGCGGACT
GAGCGAACTG
GACAAAGCAG
GATTCATTAA
ACGGCAACTT
GTGGAGACTC
GGCAGATTAC
TAAACATGTC
GCCCAAATCC
TTGACTCACG
CATGAATACC
AAGTACGACG
AAAACGACAA
ACTTATCCGC
GAGGTGAAGG
TGATTACCCT
GAAGTCCAAG
CTGGTCAGCG
ATTTCAGAAA
GGACTTTCAA
TTCTACAAAG
TGCGGGAGAT
CAATAACTAT
CATCATGCTC
ATGACGCATA
TCTGAATGCC
GTGGTGGGAA
CCGCCCTGAT
CAAGAAGTAC
CCAAAGCTGG
AAAGCGAGTT
CGTGTACGGA
GACTACAAGG
TCTACGACGT
GCGCAAGATG
ATTGCCAAAT
CTGAGCAGGA
GATCGGAAAG
GCCACCGCAA
AGTACTTCTT
CTACAGCAAC
ATCATGAATT
TCTTCAAGAC
CGAAATCACC
CTTGCAAACG
GTGAGATCCG
GAAGAGGCCG
CTCATCGAGA
CTAATGGGGA
GACTGGCGAA
ATCGTGTGGG
ACAAGGGCAG
AGATTTCGCT
ACCGTGCGCA
AAGTGCTTTC
TATGCCTCAA
GTGAACATCG
TGAAGAAAAC
CGAGGTGCAA
ACCGGAGGCT
TTTCTAAGGA
ATCAATCCTC
CCCAAGCGCA
ACTCCGACAA
GCTCATTGCA
AGGAAGAAGG
ATTGGGACCC
TAAGAAGTAC
GGCGGATTCG
ATTCACCAAC
TGTGGCTTAT
TCTGTCCTGG
TCGTGGCTAA
GGTGGAAAAA
GGAAAGTCTA
AGAAGCTCAA
GAGCGTGAAG
GAACTGCTGG
GTATCACCAT
TATGGAGCGC
AGCTCCTTCG
AGAAGAACCC
AATTGACTTT
CTCGAAGCCA
AAGGTTACAA
GGAAGTCAAG
AAGGACCTTA
TCATCAAGCT
CCCAAAGTAT
AGCCTGTTCG
AACTGGAGAA
TGGGCGGAAG
CGGATGCTCG
CCTCCGCTGG
CGAACTTCAG
AAGGGTAATG
AGCTGGCTCT
CCCCTCCAAG
TACGTGAATT
TCCTCTACCT
TGCAAGCCAT
TACGAGAAGC
TGAAGGGGAG
CCCCGAGGAC
AACGAGCAAA
AGCAACTGTT
TGTGGAGCAG
CATAAGCATT
ATCTGGACGA
GATCATTGAG
CAGATTTCCG
AGTTTTCTAA
ACGCGTCATT
CTCGCTGATG
CCAACCTCGA
TAAAGTCCTT
AGCGCATACA
ATAAGCACAG
AGACAAACCA
ATTCGGGAGC
AGGCTGAGAA
TATCATCCAC
CTGTTCACCC
TCACCAATCT
TGGTGCCCCT
GCCGCATTCA
AGTACTTCGA
CACCACCATC
GACCGGAAAC
GCTATACCTC
CACCAAAGAA
GTGCTGGACG
CCACCCTCAT
CCACCAGAGC
ATCACCGGAC
TTTACGAAAC
TCGGATTGAC
CTCTCACAGC
TCGGAGGGGA
TGAGGGAGCT
GATCCAAAAA
AGAAGAGAAA
GGTAGACCCA
AAGAAAAAAC
GAAAAGTAGA
TCCGAAAAAG
AAGAGGAAGG
TGGGATCCCT
GCCCACCTGC
AGCTGTCTTG
ATCGAGTTAT
ACAAAAAGAC
AAAGGCCCAT
ATTATACACA
CCTTGGGGCA
GGACCAAGTG
TTGCTGCTGT
CAGGGAAATC
ATGGAGAATA
GGTATGGTCA
AAAAGGAAAC
GCAATAAGGA
TAGAAATAGT
AGTGTACACC
GGTAAAGAAG
GGAAAAGCTC
TCATGGGTGT
CCAATTGCTA
AGTGGGTTTT
AAGAAGAAGC
AGTGATGAAG
AAAAAGTTCT
TTGTTTGGTC
CGGCAGCGTA
CAGGCCACCA
CTGTCCAACT
GCTGTGATGG
TGGTGCTCAT
CATGGTGTGG
GATGGCATCC
CTCTTCCAAT
GGCCGACCGG
CTATACACAG
AGCTCACAGA
GAATCTAAAG
TCATACAATG
GGCACCCTAC
CGACAGAAGA
TGCACCCTCA
ATGAAAATCG
TACCTGTACA
TGTCAAGGAA
TTGATCCAGA
GACTTGTGGA
GCTTCATTCT
CTTTTGGCTG
TTCATGGAGT
ATGTACTTTA
ATGGCTGTAA
GTTTGGTAGA
AGCCCAAGCC
CCAGAAGATT
TAGAATTGAT
CCAAGCTCTC
CCTTACATGA
AAAAAACCTT
GAAGATAACT
TACAGAGTTT
GGCTACACGA
TTAGCTCCAA
TTTATAAGCA
GTATGCTCCA
GTAGCTTACC
AAAATCAGGT
GGAATATGAA
AATGTTGCCC
GAGAATGTCG
GCTTGGCAGC
AAGGAAGGTC
GTCCCTTCTC
TGGGGTCACT
GCTTGCCTGG
ACTTCTGTGC
TCATCCCTAC
AGGGCCATTC
ACAACATGAA
TAATGGAAGC
ACTGTGGTTT
GTACCTTAAC
TCGAGAAGAT
AACCGCTCTT
TGGGTGTTAT
TCCTCAAGAT
GAGCAGCTCC
ATGTGCTACC
TCTTTATAAG
CTTTCAGACA
CAGATGAGTT
TGGCTCCAAG
GAAGGAATGG
AAGCCAAGAT
CAAATCTGGG
GCCATCGAGG
TCCTGGCACC
CCGCCGCAAA
AAAAGAACGT
GTTTCACTCA
GCCTGTTCCC
CGTTCTGGAA
AGAAGAGGGC
TGCGATGATG
ACAGAGGTTC
TTGCACATAA
GATAAGGGCA
GTGGAAAAGA
AACCTATTCC
CCGAATCAAG
CGGAAGAATA
ACTCAACAAC
AACAAACAAC
AGTAAGCCTT
CGTCACTGCC
AACCTTAGGG
AGTAACACTG
AGACCGTGCA
ACCTGAAGTA
AAAAGTGAAA
CCGAACCCCA
TTTTATCTTA
AAAAGTTCAG
ACAACACTAA
AACTTATTCG
CTGATGCCAT
CCGCTCCTCA
CCCAGTGAAA
GAGGCATCTC
CAGGCTTCTC
CTGGTCCCCG
AAGACTGCTT
CAGCCACACC
AGCTCCACTG
AAGAATGACG
CAACAGCCTC
ATGCGGGTTT
TCAGAAAGAA
GCAGCACTCC
CCACTGTACG
ATGCCTTCGG
GAAGACTCAG
TGGTGCCAAT
GCAGCTGCTG
CTGATGGCCC
TGGCATTTCA
CAGCTTGGCG
AAGTGGCTCC
TCTCCCCACC
CTGTCTGCTC
CTGTGATGGA
GCCCCTCATT
AATTCTGAGC
CTTCCACTGG
TGTGACTGAG
CCGCTAACGC
CTCATCAGCC
AAACCACCAG
CCCTCCTTCC
TCACCTCTCC
TCAAGACCTT
GCCTCTTCTC
CAATGGAAGA
AGATGAGCAG
CATTCTGAAG
CAGATGAGCC
TCCATCAGAC
GAACCCCTAT
CTGATGACCC
CCTGTCACCT
GCTGAGGAGA
AATTGCCCCA
CATTGATGAG
TATTGGTCAG
ACAGTGAGCA
CATCTTTTTG
GATGCAAATA
TTGGTGGGGT
GGCCATCGCA
CCTGCTCACG
GCTCGGTTTT
GATTGAGTGT
GCCCGGCGAG
AGCTGCACGC
TACCACTCCT
GTTGAGCACC
CCAACCGTAA
TCATCCAACC
CGCCTCTCCC
TTGTCTTTTA
CCAGCACAAA
AACCTAAATA
AGCCCCAACA
TGGTTTTGAA
CTAAACAAGA
TTAAGTTTGA
GGCTAAAGAA
GCTAAGAATA
AGAAAATGAA
GGCCTCAGAG
CAAAAAGACC
AGGCAGCTAA
TGAAGGTCCA
GAACAGTCCT
CTGAAGTAAA
TGAATTGAAC
CAAATTCCTT
CTCATAAAGC
ATTAACATTA
ACCCATGACA
ATGTTGTCAC
CGTGTCCCCT
TATGCTCTCA
CACACGTTGC
GGGGCCCTAT
AACCATTGGG
TCTGAGAATT
CGATATCAAG
CTTATCGATA
ATCAACCTCT
GGATTACAAA
ATTTGTGAAA
GATTGACTGG
TATTCTTAAC
TATGTTGCTC
CTTTTACGCT
ATGTGGATAC
GCTGCTTTAA
TGCCTTTGTA
TCATGCTATT
GCTTCCCGTA
TGGCTTTCAT
TTTCTCCTCC
TTGTATAAAT
CCTGGTTGCT
GTCTCTTTAT
GAGGAGTTGT
GGCCCGTTGT
CAGGCAACGT
GGCGTGGTGT
GCACTGTGTT
TGCTGACGCA
ACCCCCACTG
GTTGGGGCAT
TGCCACCACC
TGTCAGCTCC
TTTCCGGGAC
TTTCGCTTTC
CCCCTCCCTA
TTGCCACGGC
GGAACTCATC
GCCGCCTGCC
TTGCCCGCTG
CTGGACAGGG
GCTCGGCTGT
TGGGCACTGA
CAATTCCGTG
GTGTTGTCGG
GGAAATCATC
GTCCTTTCCT
TGGCTGCTCG
CCTGTGTTGC
CACCTGGATT
CTGCGCGGGA
CGTCCTTCTG
CTACGTCCCT
TCGGCCCTCA
ATCCAGCGGA
CCTTCCTTCC
CGCGGCCTGC
TGCCGGCTCT
GCGGCCTCTT
CCGCGTCTTC
GCCTTCGCCC
TCAGACGAGT
CGGATCTCCC
TTTGGGCCGC
CTCCCCGCAT
CGATACCGTC
GACCTCGAGA
CCTAGAAAAA
CATGGAGCAA
TCACAAGTAG
CAATACAGCA
GCTACCAATG
CTGATTGTGC
CTGGCTAGAA
GCACAAGAGG
AGGAGGAGGT
GGGTTTTCCA
GTCACACCTC
AGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ACAAGATATC
CTTGATCTGT
GGATCTACCA
CACACAAGGC
TACTTCCCTG
ATTGGCAGAA
CTACACACCA
GGGCCAGGGA
TCAGATATCC
ACTGACCTTT
GGATGGTGCT
ACAAGCTAGT
ACCAGTTGAG
CAAGAGAAGG
TAGAAGAAGC
CAATGAAGGA
GAGAACACCC
GCTTGTTACA
CCCTGTGAGC
CTGCATGGGA
TGGATGACCC
GGAGAGAGAA
GTATTAGAGT
GGAGGTTTGA
CAGCCGCCTA
GCATTTCATC
ACATGGCCCG
AGAGCTGCAT
CCGGACTGTA
CTGGGTCTCT
CTGGTTAGAC
CAGATCTGAG
CCTGGGAGCT
CTCTGGCTAA
CTAGGGAACC
CACTGCTTAA
GCCTCAATAA
AGCTTGCCTT
GAGTGCTTCA
AGTAGTGTGT
GCCCGTCTGT
TGTGTGACTC
TGGTAACTAG
AGATCCCTCA
GACCCTTTTA
GTCAGTGTGG
AAAATCTCTA
GCAGGGCCCG
TTTAAACCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
TAGGGGGTAT
CCCCACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTAATTCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCTGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTC
CCGGGAGCTT
GTATATCCAT
TTTCGGATCT
GATCAGCACG
TGTTGACAAT
TAATCATCGG
CATAGTATAT
CGGCATAGTA
TAATACGACA
AGGTGAGGAA
CTAAACCATG
GCCAAGTTGA
CCAGTGCCGT
TCCGGTGCTC
ACCGCGCGCG
ACGTCGCCGG
AGCGGTCGAG
TTCTGGACCG
ACCGGCTCGG
GTTCTCCCGG
GACTTCGTGG
AGGACGACTT
CGCCGGTGTG
GTCCGGGACG
ACGTGACCCT
GTTCATCAGC
GCGGTCCAGG
ACCAGGTGGT
GCCGGACAAC
ACCCTGGCCT
GGGTGTGGGT
GCGCGGCCTG
GACGAGCTGT
ACGCCGAGTG
GTCGGAGGTC
GTGTCCACGA
ACTTCCGGGA
CGCCTCCGGG
CCGGCCATGA
CCGAGATCGG
CGAGCAGCCG
TGGGGGCGGG
AGTTCGCCCT
GCGCGACCCG
GCCGGCAACT
GCGTGCACTT
CGTGGCCGAG
GAGCAGGACT
GACACGTGCT
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCG
ACGGATCGGG
AGATCTCCCG
ATCCCCTATG
GTGCACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGTATC
TGCTCCCTGC
TTGTGTGTTG
GAGGTCGCTG
AGTAGTGCGC
GAGCAAAATT
TA