名称: | pAf-CRISPR-phoA |
---|---|
别称: | Plasmid#207991 |
基因长: | |
质粒宿主: | 真菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 黄曲霉 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stable |
温度: | 37度 |
背景载体: | pPTRII |
正向引物: | |
反向引物: | AmpNR |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AAAAAAAAAA
AACAAGACAG
CCACATATTA
GGGAGACTAG
TTAGAAGCTA
GTTCCAGGAC
TAGGAAAATA
AAAGACAATG
ATACCACAGT
CTAGTTGACA
ACTAGATAGA
TTCTAGATTG
AGGCCAAAGT
CTCTGAGATC
CAGGTTAGTT
GCAACTAATA
CTAGTTAGTA
TCTAGTCTCC
TATAACTCTG
AAGCTAGAAT
AACTTACTAC
TATTATCCTC
ACCACTGTTC
AGCTGCGCAA
ACGGAGTGAT
TGCAAGGTGT
TCAGAGACTA
GTTATTGACT
AGTCAGTGAC
TAGCAATAAC
TAACAAGGTA
TTAACCTACC
ATGTCTGCCA
TCACCCTGCA
CTTCCTCGGG
CTCAGCAGCC
TTTTCCTCCT
CATTTTCATG
CTCATTTTCC
TTGTTTAAGA
CTGTGACTAG
TCAAAGACTA
GTCCAGAACC
ACAAAGGAGA
AATGTCTTAC
CACTTTCTTC
ATTGCTTGTC
TCTTTTGCAT
TATCCATGTC
TGCAACTAGT
TAGAGTCTAG
TTAGTGACTA
GTCCGACGAG
GACTTGCTTG
TCTCCGGATT
GTTGGAGGAA
CTCTCCAGGG
CCTCAAGATC
CACAACAGAG
CCTTCTAGAA
GACTGGTCAA
TAACTAGTTG
GTCTTTGTCT
GAGTCTGACT
TACGAGGTTG
CATACTCGCT
CCCTTTGCCT
CGTCAATCGA
TGAGAAAAAG
CGCCAAAACT
CGCAATATGG
CTTTGAACCA
CACGGTGCTG
AGACTAGTTA
GAATCTAGTC
CCAAACTAGC
TTGGATAGCT
TACCTTTGCC
CTTTGCGTTG
CGACAGGTCT
TGCAGGGTAT
GGTTCCTTTC
TCACCAGCTG
ATTTAGCTGC
CTTGCTACCC
TCACGGCGGA
TCTGCATAAA
GAGTGGCTAG
AGGTTATAAA
TTAGCACTGA
TCCTAGGTAC
GGGGCTGAAT
GTAACTTGCC
TTTCCTTTCT
CATCGCGCGG
CAAGACAGGC
TTGCTCAAAT
TCCTACCAGT
CACAGGGGTA
TGCACGGCGT
ACGGACCACT
TGAACTAGTC
ACAGATTAGT
TAGCAACTAG
TCTGCATTGA
ATGGCTGTAC
TTACGGGCCC
TCGCCATTGT
CCTGATCATT
TCCAGCTTCA
CCCTCGTTGC
TGCAAAGTAG
TTAGTGACTA
GTCAAGGACT
AGTTGAAATG
GGAGAAGAAA
CTCACGAATT
CTCGACACCC
TTAGTATTGT
GGTCCTTGGA
CTTGGTGCTG
CTATATATTA
GCTAATACAC
TAGTTAGACT
CACAGAAACT
TACGCAGCTC
GCTTGCGCTT
CTTGGTAGGA
GTCGGGGTTG
GGAGAACAGT
GCCTTCAAAC
AAGCCTTCAT
ACCATGCTAC
TTGACTAGTC
AGGGACTAGT
CACCAAGTAA
TCTAGATAGG
ACTTGCCTTT
GGCCTCCATC
AGTTCCTTCA
TAGTGGGAGG
TCCATTGTGC
AATGTAAACT
CCATGCCGTG
GGAGTTCTTG
TCCTTCAAGT
GCTTGACCAA
TATGTTTCTG
TTGGCAGAGG
GAACCTGTCA
ACTAGTTAAT
AACTAGTCAG
AAACTAGTAT
AGCAGTAGAC
TCACTGTACG
CTTGAGGCAT
CCCTTCACTC
GGCAGTAGAC
TTCATATGGA
TGGATATCAG
GCACGCCATT
GTCGTCCTGT
GGACTAGTCA
GTAACTAGGC
TTAAAGCTAG
TCGGGTCGGC
TTACTATCTT
GAAATCCGGC
AGCGTAAGCT
CCCCGTCCTT
AACTGCCTCG
AGATAGTGAC
AGTACTCTGG
GGACTTTCGG
AGATCGTTAT
CGCGAATGCT
CGGCATACTA
ATCGTTGACT
AGTCTTGGAC
TAGTCCCGAG
CAAAAAGGAT
TGGAGGAGGA
GGAGGAAGGT
GAGAGTGAGA
CAAAGAGCGA
AATAAGAGCT
TCAAAGGCTA
TCTCTAAGCA
GTATGAAGGT
TAAGTATCTA
GTTCTTGACT
AGATTTAAAA
GAGATTTCGA
CTAGTTATGT
ACCTGGAGTT
TGGATATAGG
AATGTGTTGT
GGTAACGAAA
TGTAAGGGGG
AGGAAAGAAA
AAGTCGGTCA
AGAGGTAACT
CTAAGTCGGC
CATTCCTTTT
TGGGAGGCGC
TAACCATAAA
CGGCATGGTC
GACTTAGAGT
TAGCTCAGGG
AATTTAGGGA
GTTATCTGCG
ACCACCGAGG
AACGGCGGAA
TGCCAAAGAA
TCCCGATGGA
GCTCTAGCTG
GCGGTTGACA
ACCCCACCTT
TTGGCGTTTC
TGCGGCGTTG
CAGGCGGGAC
TGGATACTTC
GTAGAACCAG
AAAGGCAAGG
CAGAACGCGC
TCAGCAAGAG
TGTTGGAAGT
GATAGCATGA
TGTGCCTTGT
TAACTAGGTC
AAAATCTGCA
ATATGCTTGA
TGTTATCCAA
AGTGTGAGAG
AGGAAGGTCC
AAACATACAC
GATTGGGAGA
GGGCCTAGGT
ATAAGAGTTT
TTGAGTAGAA
CGCATGTGAG
CCCAGCCATC
TCGAGGAGAT
TAAACACGGG
CCGGCATTTG
ATGGCTATGT
TAGTACCCCA
ATGGAAAGCC
TGAGAGTCCA
GTGGTCGCAG
ATAACTCCCT
AAATTCCCTG
AGCTAACTCT
AAGTCGACCA
TGCCGTTTAT
GGTTAGCGCC
TCCCAAAAAG
GAATGGCCGA
CTTAGAGTTA
CCTCTTGACC
GACTGCAGTT
CTCTTTAGAA
TTCAACTGTG
GGTTTTGCTT
TTTGCTTCAT
TCTCTTTGTC
TTCTCCATCT
TTGATCAAAT
CCTGGACTTT
CTCAATCCCC
AGCTAATTCA
ATCATAGTCA
GTTTTCTATT
TTTATTATTT
CTTTTTCTTT
TGAAATGTGA
TTAACAACCA
GTCTGTTATA
TATCTTGTAC
CCAGATTACG
CCCAACTCGT
GCTCCTCAGC
CACAAAGATA
CTCAATTGAT
AGCCAAGATA
CATACATACC
ACAAAGTAAG
GACTCCATGC
ATTGAGTATT
ACTCATCGTA
TTCTAGACTA
CTCCAAAACT
CAGCACATAG
ACAAACAATA
CGAACCTCGT
CTAAGGGTGA
TTCAGAGGCG
GCAAAGCGGG
GTTTTCGCAT
TTGATGTTCC
TGGCACTTAT
GTAAGCCCAC
GCTTCCCGCT
CAACTAAACC
ATCAGCCAAT
CAGACTGCTC
AGATTTATCT
TTTGAAGGGT
ATATAAATCA
TTGTAAAGAA
GAACAAGTGT
CATCCTTAGA
CGACACTCGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTATCAACTT
GAAAAAGTGG
CACCGAGTCG
GTGCTTTTTT
TTTGAGCATT
TATCAGCTTG
ATATAGAGGT
AGGAATGTAT
GGAGGTGCAG
AATGGCTATT
TTGTTATTGG
AGCGGGTTCG
AAACGGAGGG
CAGGAGACTT
TTTTTAAATA
TGTGGTACCG
TTACTAGGTT
GCAGTCAATG
CATTAATGCA
TTGCAGATGA
GCTGTATCTG
GAAGAGGTAA
ACCCGAAACG
CGTTTTATTC
TTGTTGACAT
GGAGCTATTA
AATCACTAGA
AGGCACTCTT
TGCTGCTTGG
ACAAATGAAC
GTATCTTATC
GAGATCCTGA
ACACCATTTG
TCTCAACTCC
GGAGCTGACA
TCGACACCAA
CGATCTTATA
TCCAGATTCG
TCAAGCTGTT
TGATGATTTC
AGTAACGTTA
AGTGGATCCG
GTCCGGAATT
CCCCTAGCTC
AGACCTTACG
CTTCTTCTTG
GGGTCAGCAC
GGGAGTCACC
ACCGAGCTGA
GAGAGGTCGA
TACGAGTCTC
GTAGAGACCG
GTAATGGACT
GGTGGATGAG
AGTGGCGTCG
AGGACCTCCT
TGGTAGAGGT
GTAACGCTTA
CGGTCGATGG
TGGTGTCGAA
GTACTTGAAG
GCAGCAGGGG
CACCAAGGTT
GGTGAGAGTG
AAGAGGTGGA
TGATGTTCTC
AGCCTGCTCA
CGGATGGGCT
TGTCACGGTG
CTTGTTGTAA
GCGGAGAGGA
CCTTATCGAG
GTTAGCGTCG
GCGAGGATGA
CACGCTTGGA
GAACTCGGAG
ATCTGCTCGA
TGATCTCATC
AAGGTAGTGC
TTGTGCTGCT
CGACGAAGAG
CTGCTTCTGC
TCGTTGTCTT
CGGGAGAACC
CTTGAGCTTC
TCGTAGTGGG
AGGCGAGGTA
GAGGAAGTTG
ACGTACTTGG
AGGGGAGAGC
GAGCTCGTTA
CCCTTCTGGA
GCTCACCGGC
GGAAGCGAGC
ATACGCTTAC
GACCGTTCTC
AAGCTCGAAG
AGGGAGTACT
TGGGCAGCTT
GATGATGAGG
TCCTTCTTGA
CCTCCTTGTA
ACCCTTGGCC
TCGAGGAAGT
CGATGGGGTT
CTTCTCGAAG
GAGGAACGCT
CCATGATGGT
GATGCCGAGG
AGCTCCTTGA
CGGACTTGAG
CTTCTTAGAC
TTACCCTTCT
CGACCTTGGC
GACAACGAGG
ACGGAGTAAG
CGACAGTGGG
AGAGTCGAAA
CCGCCGTACT
TCTTGGGGTC
CCAGTCCTTC
TTGCGAGCGA
TGAGCTTGTC
GGAGTTACGC
TTGGGGAGGA
TAGATTCCTT
GGAGAAGCCA
CCGGTCTGGA
CCTCGGTCTT
CTTGACGATG
TTGACCTGGG
GCATGGAGAG
GACCTTACGG
ACAGTGGCGA
AGTCACGACC
CTTGTCCCAG
ACGATCTCAC
CAGTCTCACC
GTTGGTCTCG
ATGAGGGGAC
GCTTACGGAT
CTCACCGTTG
GCGAGGGTGA
TCTCGGTCTT
GAAGAAGTTC
ATGATGTTGG
AGTAGAAGAA
GTACTTAGCG
GTGGCCTTAC
CGATCTCCTG
CTCGGACTTA
GCGATCATCT
TACGAACATC
GTAGACCTTG
TAGTCACCGT
AGACGAACTC
GGACTCCAGC
TTGGGGTACT
TCTTGATAAG
AGCAGTGCCA
ACGACAGCGT
TGAGGTAGGC
ATCGTGGGCG
TGGTGGTAGT
TGTTGATCTC
ACGGACCTTG
TAGAACTGGA
AGTCCTTACG
GAAGTCGGAG
ACGAGCTTAG
ACTTGAGAGT
GATAACCTTG
ACCTCACGGA
TGAGCTTGTC
GTTCTCATCG
TACTTGGTGT
TCATGCGAGA
GTCGAGAATC
TGGGCGACGT
GCTTGGTGAT
CTGACGAGTC
TCAACAAGCT
GACGCTTGAT
GAAGCCGGCC
TTGTCGAGCT
CAGAGAGGCC
ACCACGTTCA
GCCTTAGTGA
GGTTATCGAA
CTTACGCTGG
GTGATGAGCT
TGGCGTTGAG
AAGCTGACGC
CAGTAGTTCT
TCATCTTCTT
GACAACCTCT
TCGGAGGGGA
CGTTGTCAGA
CTTGCCACGG
TTCTTATCGG
AACGGGTGAG
GACCTTGTTG
TCGATAGAGT
CATCCTTGAG
GAAGGACTGG
GGGACGATGT
GATCGACGTC
GTAGTCGGAG
AGACGGTTGA
TGTCGAGCTC
CTGATCAACG
TACATGTCAC
GGCCGTTCTG
GAGGTAGTAG
AGGTAGAGCT
TCTCGTTCTG
AAGCTGGGTG
TTCTCGACGG
GGTGCTCCTT
AAGGATCTGG
GAGCCGAGTT
CCTTGATACC
CTCCTCGATA
CGCTTCATAC
GTTCACGAGA
GTTCTTCTGA
CCCTTCTGGG
TAGTCTGGTT
CTCACGGGCC
ATCTCGATAA
CGATGTTCTC
GGGCTTGTGA
CGACCCATAA
CCTTGACGAG
CTCATCGACG
ACCTTAACGG
TCTGGAGGAT
ACCCTTCTTG
ATAGCGGGGG
AACCGGCGAG
GTTAGCGATG
TGCTCGTGAA
GGGAGTCACC
CTGACCGGAA
ACCTGAGCCT
TCTGGATGTC
CTCCTTGAAG
GTGAGAGAGT
CGTCGTGGAT
GAGCTGCATG
AAGTTACGGT
TGGCGAAACC
ATCGGACTTA
AGGAAGTCGA
GGATAGTCTT
ACCAGACTGC
TTGTCACGGA
TGCCGTTGAT
CAGCTTACGG
GAGAGACGGC
CCCAACCAGT
GTAACGACGA
CGCTTGAGCT
GCTTCATGAC
CTTGTCGTCG
AAGAGGTGAG
CGTAAGTCTT
GAGACGTTCT
TCGATCATCT
CACGATCCTC
GAAGAGGGTA
AGGGTGAGGA
CGATGTCCTC
AAGGATGTCC
TCGTTCTCCT
CGTTGTCGAG
GAAGTCCTTG
TCCTTGATGA
TCTTGAGGAG
ATCGTGGTAG
GTGCCGAGGG
AAGCGTTGAA
ACGATCCTCG
ACACCGGAGA
TCTCAACGGA
GTCGAAGCAC
TCGATCTTCT
TGAAGTAGTC
TTCCTTGAGC
TGCTTGACGG
TAACCTTACG
GTTGGTCTTG
AAGAGGAGGT
CGACGATAGC
CTTCTTCTGC
TCACCAGAGA
GGAAGGCGGG
CTTACGCATA
CCTTCGGTGA
CGTACTTGAC
CTTGGTGAGC
TCGTTGTAAA
CAGTGAAGTA
CTCGTAAAGG
AGAGAGTGCT
TAGGAAGGAC
CTTCTCGTTA
GGGAGGTTCT
TGTCGAAGTT
AGTCATACGC
TCGATGAAGG
ACTGGGCAGA
GGCACCCTTA
TCGACGACTT
CCTCGAAGTT
CCAGGGAGTG
ATGGTCTCTT
CGGACTTACG
AGTCATCCAG
GCGAAACGGG
AGTTACCACG
GGCGAGGGGG
CCAACGTAGT
AGGGGATACG
GAAGGTGAGG
ATCTTCTCGA
TCTTCTCACG
GTTGTCCTTG
AGGAAGGGGT
AGAAGTCCTC
CTGACGACGG
AGGATAGCGT
GGAGTTCGCC
AAGGTGGATC
TGGTGGGGGA
TGGAACCGTT
GTCGAAAGTA
CGCTGCTTAC
GGAGGAGATC
CTCACGGTTG
AGCTTGACGA
GGAGCTCCTC
GGTGCCGTCC
ATCTTCTCGA
GGATGGGCTT
GATGAACTTG
TAGAACTCCT
CCTGGGAAGC
ACCGCCGTCG
ATGTAACCGG
CGTAACCGTT
CTTGGACTGA
TCGAAGAAGA
TCTCCTTGTA
CTTCTCAGGG
AGCTGCTGAC
GGACAAGGGC
CTTGAGAAGA
GTGAGGTCCT
GGTGGTGCTC
ATCGTAACGC
TTGATCATAG
AAGCGGAGAG
GGGAGCCTTG
GTGATCTCGG
TGTTGACACG
GAGGATATCG
GAGAGGAGGA
TGGCGTCGGA
GAGGTTCTTA
GCGGCAAGGA
AGAGGTCAGC
GTACTGGTCA
CCGATCTGGG
CGAGGAGGTT
GTCGAGATCG
TCATCGTAGG
TGTCCTTGGA
GAGCTGGAGT
TTGGCATCCT
CGGCGAGGTC
GAAGTTGGAC
TTGAAGTTGG
GGGTGAGACC
GAGAGAGAGG
GCGATAAGGT
TACCGAAGAG
ACCGTTCTTC
TTCTCACCAG
GGAGCTGAGC
GATGAGGTTC
TCGAGACGAC
GGGACTTGGA
GAGACGAGCG
GAGAGGATGG
CCTTGGCGTC
AACACCGGAA
GCGTTGATGG
GGTTCTCTTC
GAAGAGCTGG
TTGTAAGTCT
GAACGAGCTG
GATGAAGAGC
TTGTCGACAT
CGGAGTTGTC
AGGGTTGAGG
TCACCCTCGA
TGAGGAAGTG
ACCACGGAAC
TTGATCATGT
GGGCGAGGGC
GAGGTAGATG
AGACGAAGGT
CAGCCTTATC
AGTAGAGTCG
ACCAGCTTCT
TACGGAGGTG
GTAGATGGTG
GGGTACTTCT
CGTGGTAGGC
GACCTCGTCG
ACGATGTTAC
CGAAGATGGG
GTGACGCTCG
TGCTTCTTAT
CCTCCTCGAC
GAGGAAGGAC
TCCTCGAGAC
GGTGGAAGAA
GGAGTCATCG
ACCTTAGCCA
TCTCGTTGGA
GAAGATCTCC
TGGAGGTAGC
AGATACGGTT
CTTACGACGG
GTGTAACGAC
GACGGGCGGT
ACGCTTGAGA
CGGGTGGCCT
CGGCGGTCTC
ACCGGAGTCG
AAGAGGAGGG
CACCGATGAG
GTTCTTCTTG
ATGGAGTGAC
GGTCGGTGTT
ACCGAGGACC
TTGAACTTCT
TGGAGGGGAC
CTTGTACTCG
TCGGTGATGA
CGGCCCAACC
GACGGAGTTG
GTGCCGATGT
CGAGACCGAT
GGAGTACTTC
TTGTCCATGC
GGCCGGCTGC
TGGGACTCCG
TGGATACCGA
CCTTCCGCTT
CTTCTTTGGG
GCCATGATCA
TGCGGCCGCC
TGCTCAAGCG
GGGTAGCTGT
TAGTCAAGCT
GCGATGAAGT
GGGAAAGCTC
GAACTGAAAG
GTTCAAAGGA
ATAAGGGATG
GGAAGGATGG
AGTATGGATG
TAGCAAAGTA
CTTACTTAGG
GGAAATAAAG
GTTCTTGGAT
GGGAAGATGA
ATATACTGAA
GATGGGAAAA
GAAAGAGAAA
AGAAAAGAGC
AGCTGGTGGG
GAGAGCAGGA
AAATATGGCA
ACAAATGTTG
GACTGACGCA
ACGACCTTGT
CAACCCCGCC
GACACACCGG
GCGGACAGAC
GGGGCAAAGC
TGCCTACCAG
GGACTGAGGG
ACCTCAGCAG
GTCGAGTGCA
GAGCACCGGA
TGGGTCGACT
GCCAGCTTGT
GTTCCCGGTC
TGCGCCGCTG
GCCAGCTCCT
GAGCGGCCTT
TCCGGTTTCA
TACACCGGGC
AAAGCAGGAG
AGGCACGATA
TTTGGACGCC
CTACAGATGC
CGGATGGGCC
AATTAGGGAG
CTTACGCGCC
GGGTACTCGC
TCTACCTACT
TCGGAGAAGG
TACTATCTCG
TGAATCTTTT
ACCAGATCGG
AAGCAATTGG
ACTTCTGTAC
CTAGGTTAAT
GGCATGCTAT
TTCGCCGACG
GCTATACACC
CCTGGCTTCA
CATTCTCCTT
CGCTTACTGC
CGGTGATTCG
ATGAAGCTCC
ATATTCTCCG
ATGATGCAAT
AGATTCTTGG
TCAACGAGGG
GCACACCAGC
CTTTCCACTT
CGGGGCGGAG
GGGCGGCCGG
TCCCGGATTA
ATAATCATCC
ACTGCACCTC
AGAGCCGCCA
GAGCTGTCTG
GCGCAGTGGC
GCTTATTACT
CAGCCCTTCT
CTCTGCGTCC
GTCCGTCTCT
CCGCATGCCA
GAAAGAGTCA
CCGGTCACTG
TACAGAGCTC
ACGAGTTCGT
CACATTTTTC
TACAAATGGT
GGAGGCGGCG
GATTTTAGGC
TCAAGTCATG
ACCCTCTGGG
TCACTCCAGA
ATCAGCTAGG
TCAACGAATA
AGGATGATTC
TATAGGAAGA
TCCAGGCACC
GGTCAACCAT
GATCTGGACA
GATTTGGGAG
CTCGGTATAA
GCTCTCCACC
ATCTTATTCT
GTATAGTTTA
GGCTTAAAGT
TTATCCAGGA
GATGTTGCTG
AAGTCGATTT
GAGTCCACTT
CCTCACTGGT
AGCTATACGA
CTTTGATGGT
CGTTGTAGGG
GCTGTATTAG
GTCTCGATCA
AACACAAATA
GAATTAAATG
GTACTCGAAG
CTTGGCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCTGCATTAA
TGAATCGGCC
AACGCGCGGG
GAGAGGCGGT
TTGCGTATTG
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGAA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTACAGGCAT
CGTGGTGTCA
CGCTCGTCGT
TTGGTATGGC
TTCATTCAGC
TCCGGTTCCC
AACGATCAAG
GCGAGTTACA
TGATCCCCCA
TGTTGTGCAA
AAAAGCGGTT
AGCTCCTTCG
GTCCTCCGAT
CGTTGTCAGA
AGTAAGTTGG
CCGCAGTGTT
ATCACTCATG
GTTATGGCAG
CACTGCATAA
TTCTCTTACT
GTCATGCCAT
CCGTAAGATG
CTTTTCTGTG
ACTGGTGAGT
ACTCAACCAA
GTCATTCTGA
GAATAGTGTA
TGCGGCGACC
GAGTTGCTCT
TGCCCGGCGT
CAATACGGGA
TAATACCGCG
CCACATAGCA
GAACTTTAAA
AGTGCTCATC
ATTGGAAAAC
GTTCTTCGGG
GCGAAAACTC
TCAAGGATCT
TACCGCTGTT
GAGATCCAGT
TCGATGTAAC
CCACTCGTGC
ACCCAACTGA
TCTTCAGCAT
CTTTTACTTT
CACCAGCGTT
TCTGGGTGAG
CAAAAACAGG
AAGGCAAAAT
GCCGCAAAAA
AGGGAATAAG
GGCGACACGG
AAATGTTGAA
TACTCATACT
CTTCCTTTTT
CAATGGGCAA
TTGATTACGG
GATCCCATTG
GTAACGAAAT
GTAAAAGCTA
GGAGATCGTC
CGCCGATGTC
AGGATGATTT
CACTTGTTTC
TTGTCCGGCT
CACCGGTCAA
AGCTAAAGAG
GAGCAAAAGG
AACGGATAGA
ATCGGGTGCC
GCTGATCTAT
ACGGTATAGT
GCCCTTATCA
CGTTGACTCA
ACCCATGCTA
TTTAACTCAA
CCCCTCCTTC
TGAACCCCAC
CATCTTCTTC
CTTTTCCTCT
CATCCCACAC
AATTCTCTAT
CTCAGATTTG
AATTCCAAAA
GTCCTCGGAC
GAAACTGAAC
AAGTCTTCCT
CCCTTCGATA
AACCTTTGGT
GATTGGAATA
ACTGACCATC
TTCTATAGTT
CCCAAACCAA
CCGACAATGT
AAATACACTC
CTCGATTAGC
CCTCTAGAGG
GCATACGATG
GAAGTCATGG
AATACTTTTG
GCTGGACTCT
CACAATGATC
AAGGTATCTT
AGGTAACGTC
TTTGGCGTGG
GCCGGTGTTC
GTTCCCAGTC
ATCGATGCAT
TCACATGCCC
TCCCTAAGCT
GGGCCCTAGA
CTCTAGGATC
CTAGTCTAGA
AGGACATGGC
ATCGATGGAC
TGGGTTCGTT
CTGAGATTAT
ACGGCTAAAA
CTTGATCTGG
ATAATACCAG
CGAAAAGGGT
CATGCCTTCT
CTCGTTCTTC
CTGTTGATGG
AATGGCTAAC
AGATGATAGT
CATTGCAACT
TGAAACATGT
CTCCTCCAGC
TGCCATCTAC
GAACCCACTG
TGGCCGCTAC
CGGCCTCAAG
GGTAAGGTCG
TGGTTTCTGA
GACCGTCCCC
GTTGAGGGAG
CTTCTCAGAC
CAAGCTGTTG
GACCATTTCG
GTGGCAAGTG
GGACGAGTTC
AAGTTCGCCC
CTATCCGCGA
AAGCCAGGTC
TCTCGTGCCA
TGACCAGACG
TTACTTTGAG
GACCTGGACA
AGTACGCTGA
AAGTGACGTT
GTCATTGTTG
GTGCTGGTTC
CTGCGGTCTG
AGCACTGCGT
ACGTCTTGGC
CAAGGCTCGT
CCGGACCTGA
AGATTGCTAT
CGTCGAGGCC
AGCGTCTCTC
CTGGTCAGTA
GTCCATGATG
GATTGCCTTG
CACTCAGCTT
TCCGGAACTA
ACGTGCAATA
GGTGGCGGTG
CCTGGTTGGG
TGGCCAACTC
TTTTCTGCTA
TGGTCATGCG
CCGTCCCGCG
GAAGTCTTCC
TGAACGAGCT
GGGTGTTCCT
TACGAAGAGG
ACGCAAACCC
CAACTACGTT
GTCGTCAAGC
ACGCCTCCCT
GTTTACCTCG
ACACTCATGT
CGAAGGTTCT
CTCCTTCCCC
AATGTCAAGC
TCTTCAATGC
TACCGCTGTT
GAGGACTTGA
TCACCCGTCC
GACCGAGAAC
GGCAACCCCC
AGATTGCTGG
TGTTGTCGTC
AACTGGACGC
TGGTCACCCT
TCACCACGAT
GATCACTCCT
GCATGGACCC
CAACACTATC
AACGCTCCTG
TCATCATCAG
TACCACTGGT
CACGATGGGC
CATTCGGCGC
CTTCTGTGCG
AAGCGCTTGG
TGTCCATGGG
CAGCGTCGAC
AAGCTAGGTG
GCATGCGTGG
TCTCGACATG
AGCTCGGCCG
AGGATGCCAT
CGTCAAGAAC
ACCCGCGAGG
TTACTAAGGG
CTTGATAATC
GGCGGTATGG
AGCTGTCTGA
AATTGATGGC
TTTAACCGCA
TGGGCCCTAC
CTTCGGTGCC
ATGGTTCTCA
GTGGTGTCAA
GGCTGCCGAG
GAGGCATTGA
AGGTGTTCGA
CGAGCGTCAG
CGCGAGTGTG
CTGAGTAAAT
GACTCACTAC
CCGAATGGGT
TCAGTGCATG
AACCGGATTT
GTCTTACGGT
CTTTGACGAT
AGGGGAATGA
TGATTATGTG
ATAGTTCTGA
GATTTGAATG
AACTCGTTAG
CTCGTAATCC
ACATGCATAT
GTAAATGGCT
GTGTCCCGTA
TGTAACGGTG
GGGCATTCTA
GAATAATTAT
GTGTAACAAG
AAAGACAGTA
TAATACAAAC
AAAGATGCAA
GAGCGGCTCA
TCGTCACCCC
ATTTTGTTAA
AATTCGCGTT
AAATTTTTGT
TAAATCAGCT
CATTTTTTAA
CCAATAGACC
GAAATCGGCA
AAATCCCTTA
TAAATCAAAA
GAATAGCCCG
AGATAGAGTT
GAGTGTTGTT
CCAGTTTGGA
ACAAGAGTCC
ACTATTAAAG
AACGTGGACT
CCAACGTCAA
AGGGCGAAAA
ACCGTCTATC
AGGGCGATGG
CCCACTACGT
GAACCATCAC
CCAAATCAAG
TTTTTTGGGG
TCGAGGTGCC
GTAAAGCACT
AAATCGGAAC
CCTAAAGGGA
GCCCCCGATT
TAGAGCTTGA
CGGGGAAAGC
CAGCTTATTT
TTTGTATACT
GTTTTGTGAT
AGCACGAAGT
TTTTCCACGG
TATCTTGTTA
AAAATATATA
TTTGTGGCGG
GCTTACCTAC
ATCAAATTAA
TAAGAGACTA
ATTATAAACT
AAACACACAA
GCAAGCTACT
TTAGGGTAAA
AGTTTATAAA
TGCTTTTGAC
GTATAAACGT
TGCTTGTATT
TATTATTACA
ATTAAAGGTG
GATAGAAAAC
CTAGAGACTA
GTTAGAAACT
AATCTCAGGT
TTGCGTTAAA
CTAAATCAGA
GCCCGAGAGG
TTAACAGAAC
CTAGAAGGGG
ACTAGATATC
CGGGTAGGGA
AAC