名称: | pLV3-U6-EBNA-2(human)-sgRNA2-BFP-Puro |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 蓝色 |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | LentiGuide-Puro |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCCGGGTGCA
AAGATGGATA
AAGTTTTAAA
CAGAGAGGAA
TCTTTGCAGC
TAATGGACCT
TCTAGGTCTT
GAAAGGAGTG
GGAATTGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGATCCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACCTGGCT
GCAGTACGTG
ATTCTTGATC
CCGAGCTTCG
GGTTGGAAGT
GGGTGGGAGA
GTTCGAGGCC
TTGCGCTTAA
GGAGCCCCTT
CGCCTCGTGC
TTGAGTTGAG
GCCTGGCCTG
GGCGCTGGGG
CCGCCGCGTG
CGAATCTGGT
GGCACCTTCG
CGCCTGTCTC
GCTGCTTTCG
ATAAGTCTCT
AGCCATTTAA
AATTTTTGAT
GACCTGCTGC
GACGCTTTTT
TTCTGGCAAG
ATAGTCTTGT
AAATGCGGGC
CAAGATCTGC
ACACTGGTAT
TTCGGTTTTT
GGGGCCGCGG
GCGGCGACGG
GGCCCGTGCG
TCCCAGCGCA
CATGTTCGGC
GAGGCGGGGC
CTGCGAGCGC
GGCCACCGAG
AATCGGACGG
GGGTAGTCTC
AAGCTGGCCG
GCCTGCTCTG
GTGCCTGGCC
TCGCGCCGCC
GTGTATCGCC
CCGCCCTGGG
CGGCAAGGCT
GGCCCGGTCG
GCACCAGTTG
CGTGAGCGGA
AAGATGGCCG
CTTCCCGGCC
CTGCTGCAGG
GAGCTCAAAA
TGGAGGACGC
GGCGCTCGGG
AGAGCGGGCG
GGTGAGTCAC
CCACACAAAG
GAAAAGGGCC
TTTCCGTCCT
CAGCCGTCGC
TTCATGTGAC
TCCACGGAGT
ACCGGGCGCC
GTCCAGGCAC
CTCGATTAGT
TCTCGAGCTT
TTGGAGTACG
TCGTCTTTAG
GTTGGGGGGA
GGGGTTTTAT
GCGATGGAGT
TTCCCCACAC
TGAGTGGGTG
GAGACTGAAG
TTAGGCCAGC
TTGGCACTTG
ATGTAATTCT
CCTTGGAATT
TGCCCTTTTT
GAGTTTGGAT
CTTGGTTCAT
TCTCAAGCCT
CAGACAGTGG
TTCAAAGTTT
TTTTCTTCCA
TTTCAGGTGT
CGTGACGTAC
GGCCACCATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCCGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGAGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCGGATCC
GGCGCAACAA
ACTTCTCTCT
GCTGAAACAA
GCCGGAGATG
TCGAAGAGAA
TCCTGGACCG
ATGGTGAGCA
AGGGCGAGGA
GCTGTTCACC
GGGGTGGTGC
CCATCCTGGT
CGAGCTGGAC
GGCGACGTAA
ACGGCCACAA
GTTCAGCGTG
TCCGGCGAGG
GCGAGGGCGA
TGCCACCTAC
GGCAAGCTGA
CCCTGAAGTT
CATCTGCACC
ACCGGCAAGC
TGCCCGTGCC
CTGGCCCACC
CTCGTGACCA
CCCTGAGCCA
CGGCGTCCAG
TGCTTCAGCC
GCTACCCCGA
CCACATGAAG
CAGCACGACT
TCTTCAAGTC
CGCCATGCCC
GAAGGCTACG
TCCAGGAGCG
CACCATCTTC
TTCAAGGACG
ACGGCAACTA
CAAGACCCGC
GCCGAGGTGA
AGTTCGAGGG
CGACACCCTG
GTGAACCGCA
TCGAGCTGAA
GGGCATCGAC
TTCAAGGAGG
ACGGCAACAT
CCTGGGGCAC
AAGCTGGAGT
ACAACTTCAA
CAGCCACAAC
GTCTATATCA
TGGCCGACAA
GCAGAAGAAC
GGCATCAAGG
CGAACTTCAA
GATCCGCCAC
AACATCGAGG
ACGGCAGCGT
GCAGCTCGCC
GACCACTACC
AGCAGAACAC
CCCCATCGGC
GACGGCCCCG
TGCTGCTGCC
CGACAGCCAC
TACCTGAGCA
CCCAGTCCGC
CCTGAGCAAA
GACCCCAACG
AGAAGCGCGA
TCACATGGTC
CTGCTGGAGT
TCGTGACCGC
CGCCGGGATC
ACTCTCGGCA
TGGACGAGCT
GTACAAGTAA
GTCGACAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AATCATCGTC
CTTTCCTTGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCGTCGA
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAGACAAGA
TCTGCTTTTT
GCTTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTACGTATAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGC
TCTAGCTATC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGGA
CGTACCCAAT
TCGCCCTATA
GTGAGTCGTA
TTACGCGCGC
TCACTGGCCG
TCGTTTTACA
ACGTCGTGAC
TGGGAAAACC
CTGGCGTTAC
CCAACTTAAT
CGCCTTGCAG
CACATCCCCC
TTTCGCCAGC
TGGCGTAATA
GCGAAGAGGC
CCGCACCGAT
CGCCCTTCCC
AACAGTTGCG
CAGCCTGAAT
GGCGAATGGG
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTT
TAACAAAATA
TTAACGCTTA
CAATTTAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTTCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAGAGC
GCCCAATACG
CAAACCGCCT
CTCCCCGCGC
GTTGGCCGAT
TCATTAATGC
AGCTGGCACG
ACAGGTTTCC
CGACTGGAAA
GCGGGCAGTG
AGCGCAACGC
AATTAATGTG
AGTTAGCTCA
CTCATTAGGC
ACCCCAGGCT
TTACACTTTA
TGCTTCCGGC
TCGTATGTTG
TGTGGAATTG
TGAGCGGATA
ACAATTTCAC
ACAGGAAACA
GCTATGACCA
TGATTACGCC
AAGCGCGCAA
TTAACCCTCA
CTAAAGGGAA
CAAAAGCTGG
AGCTGCAAGC
TTAATGTAGT
CTTATGCAAT
ACTCTTGTAG
TCTTGCAACA
TGGTAACGAT
GAGTTAGCAA
CATGCCTTAC
AAGGAGAGAA
AAAGCACCGT
GCATGCCGAT
TGGTGGAAGT
AAGGTGGTAC
GATCGTGCCT
TATTAGGAAG
GCAACAGACG
GGTCTGACAT
GGATTGGACG
AACCACTGAA
TTGCCGCATT
GCAGAGATAT
TGTATTTAAG
TGCCTAGCTC
GATACATAAA
CGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTGGCGCC
CGAACAGGGA
CTTGAAAGCG
AAAGGGAAAC
CAGAGGAGCT
CTCTCGACGC
AGGACTCGGC
TTGCTGAAGC
GCGCACGGCA
AGAGGCGAGG
GGCGGCGACT
GGTGAGTACG
CCAAAAATTT
TGACTAGCGG
AGGCTAGAAG
GAGAGAGATG
GGTGCGAGAG
CGTCAGTATT
AAGCGGGGGA
GAATTAGATC
GCGATGGGAA
AAAATTCGGT
TAAGGCCAGG
GGGAAAGAAA
AAATATAAAT
TAAAACATAT
AGTATGGGCA
AGCAGGGAGC
TAGAACGATT
CGCAGTTAAT
CCTGGCCTGT
TAGAAACATC
AGAAGGCTGT
AGACAAATAC
TGGGACAGCT
ACAACCATCC
CTTCAGACAG
GATCAGAAGA
ACTTAGATCA
TTATATAATA
CAGTAGCAAC
CCTCTATTGT
GTGCATCAAA
GGATAGAGAT
AAAAGACACC
AAGGAAGCTT
TAGACAAGAT
AGAGGAAGAG
CAAAACAAAA
GTAAGACCAC
CGCACAGCAA
GCGGCCGCTG
ATCTTCAGAC
CTGGAGGAGG
AGATATGAGG
GACAATTGGA
GAAGTGAATT
ATATAAATAT
AAAGTAGTAA
AAATTGAACC
ATTAGGAGTA
GCACCCACCA
AGGCAAAGAG
AAGAGTGGTG
CAGAGAGAAA
AAAGAGCAGT
GGGAATAGGA
GCTTTGTTCC
TTGGGTTCTT
GGGAGCAGCA
GGAAGCACTA
TGGGCGCAGC
GTCAATGACG
CTGACGGTAC
AGGCCAGACA
ATTATTGTCT
GGTATAGTGC
AGCAGCAGAA
CAATTTGCTG
AGGGCTATTG
AGGCGCAACA
GCATCTGTTG
CAACTCACAG
TCTGGGGCAT
CAAGCAGCTC
CAGGCAAGAA
TCCTGGCTGT
GGAAAGATAC
CTAAAGGATC
AACAGCTCCT
GGGGATTTGG
GGTTGCTCTG
GAAAACTCAT
TTGCACCACT
GCTGTGCCTT
GGAATGCTAG
TTGGAGTAAT
AAATCTCTGG
AACAGATTTG
GAATCACACG
ACCTGGATGG
AGTGGGACAG
AGAAATTAAC
AATTACACAA
GCTTAATACA
CTCCTTAATT
GAAGAATCGC
AAAACCAGCA
AGAAAAGAAT
GAACAAGAAT
TATTGGAATT
AGATAAATGG
GCAAGTTTGT
GGAATTGGTT
TAACATAACA
AATTGGCTGT
GGTATATAAA
ATTATTCATA
ATGATAGTAG
GAGGCTTGGT
AGGTTTAAGA
ATAGTTTTTG
CTGTACTTTC
TATAGTGAAT
AGAGTTAGGC
AGGGATATTC
ACCATTATCG
TTTCAGACCC
ACCTCCCAAC
CCCGAGGGGA
CCCAGAGAGG
GCCTATTTCC
CATGATTCCT
TCATATTTGC
ATATACGATA
CAAGGCTGTT
AGAGAGATAA
TTAGAATTAA
TTTGACTGTA
AACACAAAGA
TATTAGTACA
AAATACGTGA
CGTAGAAAGT
AATAATTTCT
TGGGTAGTTT
GCAGTTTTAA
AATTATGTTT
TAAAATGGAC
TATCATATGC
TTACCGTAAC
TTGAAAGTAT
TTCGATTTCT
TGGCTTTATA
TATCTTGTGG
AAAGGACGAA
ACACCGGCAT
CTCCCTGTCT
TGCATGGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTAA
GCTTGGCGTA
ACTAGATCTT
GAGACAAATG
GCAGTATTCA
TCCACAATTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTCGGGT
TTATTACAGG
GACAGCAGAG
ATCCACTTTG
GCGCCGGCTC
GAGGGGG