产品货号：P68272
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pKLAC1-miR162a-IGG6-DCL1(arabidopsis)-HA |
|---|---|
| 别称: | NM_099986.4;ABNORMAL SUSPENSOR 1; ASU1; ATDCL1; CAF;CARPEL FACTORY; dicer-like 1; DICER-LIKE 1; EMB60; EMB76;EMBRYO DEFECTIVE 60; EMBRYO DEFECTIVE 76; SHORT INTEGUMENTS 1; |
| 基因长: | 5730bp |
| 质粒宿主： | 酵母菌 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 拟南芥 |
| 原核抗性： | Amp |
| 筛选标记： | |
| 荧光标记： | |
| 启动子: | |
| 复制子: | pUC |
| 拷贝数: | 低 |
| 感受态: | BL21(DE3) |
| 温度: | 30度 |
| 背景载体: | pKLAC1 |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
AAGCTTGAAA
AAAATGAAAT
TCTCTACTAT
ATTAGCCGCA
TCTACTGCTT
TAATTTCCGT
TGTTATGGCT
GCTCCAGTTT
CTACCGAAAC
TGACATCGAC
GATCTTCCAA
TATCGGTTCC
AGAAGAAGCC
TTGATTGGAT
TCATTGACTT
AACCGGGGAT
GAAGTTTCCT
TGTTGCCTGT
TAATAACGGA
ACCCACACTG
GTATTCTATT
CTTAAACACC
ACCATCGCTG
AAGCTGCTTT
CGCTGACAAG
GATGATCTCG
AGAAAAGAGA
GGCTGAAGCT
AGAAGAGCTA
GATCTCCTAG
GGGTACCTAG
TTGGAAGAAG
AGTGAGAGTC
GCTGGAGGCA
GCGGTTCATC
GATCTCTTCC
TGTGAACACA
TTAAAAATGT
AAAAGCATGA
ATAGATCGAT
AAACCTCTGC
ATCCAGCGTT
TGCCTCTTGT
ATCTTTCTTA
TTGACTTCAA
TCAAACATGG
TAATGGAGGA
TGAGCCTAGA
GAAGCCACAA
TAAAGCCTTC
TTATTGGCTA
GATGCTTGCG
AGGACATCTC
TTGTGATCTT
ATCGATGATC
TCGTGTCTGA
ATTTGATCCT
TCCTCTGTTG
CTGTCAATGA
ATCCACTGAT
GAAAACGGCG
TCATCAATGA
TTTTTTCGGT
GGGATTGATC
ACATTTTAGA
TAGTATCAAG
AACGGTGGAG
GCTTACCAAA
CAATGGCGTT
TCTGATACCA
ATTCTCAAAT
CAACGAGGTT
ACTGTAACTC
CTCAGGTTAT
TGCTAAGGAG
ACAGTGAAGG
AGAATGGGTT
GCAAAAGAAT
GGCGGTAAGA
GAGACGAATT
CTCGAAAGAG
GAAGGAGACA
AGGATAGGAA
GAGAGCTAGG
GTTTGTAGTT
ATCAGAGTGA
AAGGAGTAAC
CTTTCAGGTA
GAGGGCATGT
TAATAATTCT
AGGGAGGGAG
ATAGGTTTAT
GAATAGGAAA
CGTACTCGTA
ATTGGGACGA
GGCGGGTAAC
AATAAGAAGA
AAAGGGAATG
TAACAATTAC
AGAAGAGATG
GTAGAGATAG
AGAAGTTAGG
GGTTATTGGG
AGAGGGATAA
AGTTGGTTCC
AATGAGTTGG
TTTATAGGTC
AGGGACTTGG
GAAGCTGATC
ATGAAAGAGA
TGTTAAGAAA
GTGAGTGGTG
GAAACCGCGA
ATGCGATGTC
AAGGCAGAGG
AGAACAAGAG
TAAGCCTGAA
GAACGTAAAG
AGAAGGTTGT
GGAAGAGCAA
GCAAGGCGAT
ACCAGTTGGA
TGTTCTTGAA
CAAGCTAAAG
CGAAAAACAC
GATTGCTTTC
CTTGAGACCG
GTGCTGGAAA
GACACTTATC
GCGATTCTTC
TTATTAAAAG
TGTTCATAAG
GATCTGATGA
GCCAGAACAG
AAAAATGCTC
TCGGTGTTCT
TGGTTCCCAA
AGTGCCTTTG
GTTTATCAGC
AAGCAGAAGT
GATCCGTAAT
CAAACTTGTT
TTCAAGTTGG
ACATTATTGT
GGTGAGATGG
GACAGGACTT
TTGGGATTCT
CGAAGGTGGC
AACGAGAGTT
TGAGTCTAAG
CAGGTTCTAG
TTATGACAGC
ACAAATTCTG
TTGAATATAC
TGAGACACAG
TATCATTAGA
ATGGAAACAA
TTGATCTTCT
TATTCTCGAC
GAGTGTCACC
ACGCTGTCAA
GAAACATCCA
TACTCTTTAG
TGATGTCAGA
GTTTTACCAT
ACAACTCCTA
AAGATAAAAG
ACCTGCCATC
TTTGGAATGA
CTGCTTCGCC
TGTTAATTTA
AAGGGTGTTT
CAAGCCAAGT
AGATTGTGCG
ATAAAGATAC
GTAACCTCGA
GACCAAGTTG
GATTCTACGG
TTTGTACTAT
AAAAGATCGA
AAAGAATTAG
AGAAACATGT
GCCTATGCCT
TCAGAGATAG
TCGTCGAGTA
TGACAAAGCT
GCTACTATGT
GGTCTCTTCA
TGAGACAATA
AAGCAAATGA
TTGCAGCTGT
TGAAGAAGCG
GCACAAGCAA
GTTCAAGGAA
AAGCAAGTGG
CAATTTATGG
GGGCTAGGGA
TGCTGGAGCA
AAGGATGAAT
TGAGACAGGT
TTATGGCGTC
TCTGAAAGAA
CGGAGAGCGA
TGGTGCTGCC
AATTTGATTC
ATAAACTTAG
AGCTATCAAT
TATACTCTTG
CTGAATTGGG
TCAATGGTGT
GCTTACAAGG
TGGGACAATC
ATTCTTGTCT
GCTTTGCAAA
GTGATGAGAG
GGTGAATTTC
CAAGTCGACG
TGAAGTTTCA
AGAATCATAC
CTCAGTGAGG
TGGTGTCACT
CTTGCAATGT
GAGCTTCTGG
AAGGCGCTGC
TGCTGAAAAA
GTCGCGGCGG
AAGTTGGCAA
ACCAGAAAAT
GGTAATGCAC
ATGACGAGAT
GGAGGAGGGA
GAGCTCCCTG
ATGATCCTGT
GGTCTCGGGA
GGGGAGCACG
TTGATGAAGT
AATAGGCGCC
GCAGTGGCTG
ATGGGAAAGT
TACTCCAAAA
GTACAATCAT
TGATCAAACT
ACTCCTCAAA
TATCAGCACA
CAGCTGATTT
TCGAGCTATT
GTTTTCGTTG
AGAGGGTGGT
TGCTGCTTTG
GTTCTTCCTA
AGGTTTTTGC
GGAGCTGCCT
TCGCTTAGTT
TTATACGGTG
TGCCAGCATG
ATTGGACACA
ATAACAGCCA
GGAGATGAAA
TCATCTCAAA
TGCAGGATAC
AATTTCCAAA
TTCCGAGATG
GGCATGTGAC
ACTGTTAGTT
GCCACAAGCG
TTGCTGAGGA
AGGACTTGAT
ATTAGGCAAT
GTAACGTTGT
TATGCGTTTC
GACCTTGCAA
AGACGGTGCT
GGCATACATT
CAGTCTCGTG
GCCGGGCAAG
AAAGCCTGGA
TCAGACTACA
TACTCATGGT
TGAGAGAGGA
AATGTATCTC
ACGCAGCGTT
CCTAAGGAAT
GCTAGGAACA
GTGAGGAGAC
ACTTCGAAAA
GAAGCAATAG
AAAGGACTGA
TCTTAGTCAT
CTCAAAGATA
CATCGAGATT
AATCTCAATT
GATGCTGTGC
CTGGTACAGT
TTATAAGGTG
GAGGCAACTG
GTGCCATGGT
TAGCTTGAAT
TCCGCGGTTG
GTCTTGTACA
TTTCTACTGC
TCTCAGCTTC
CTGGTGACAG
GTATGCAATC
CTTCGTCCTG
AGTTTAGCAT
GGAGAAGCAT
GAAAAGCCTG
GGGGCCACAC
GGAATATTCA
TGTAGGCTTC
AGCTTCCTTG
CAATGCACCG
TTTGAAATAC
TTGAGGGTCC
TGTTTGCAGT
TCAATGCGTC
TTGCACAACA
GGCTGTATGT
TTAGCTGCTT
GCAAGAAACT
GCATGAGATG
GGTGCATTTA
CCGATATGCT
ATTACCGGAC
AAAGGAAGTG
GTCAAGACGC
TGAGAAGGCT
GACCAAGATG
ATGAAGGTGA
GCCTGTTCCT
GGAACTGCTA
GACATAGAGA
GTTCTATCCT
GAAGGTGTGG
CGGATGTACT
TAAGGGAGAA
TGGGTTTCAT
CTGGAAAGGA
AGTTTGTGAG
AGCTCAAAGC
TATTCCATTT
ATACATGTAT
AATGTCAGAT
GTGTAGATTT
TGGCTCTTCA
AAAGATCCAT
TCCTAAGCGA
AGTTTCAGAG
TTCGCGATTC
TTTTTGGCAA
TGAGCTGGAT
GCAGAGGTAT
TATCGATGTC
TATGGATCTT
TATGTTGCTC
GGGCCATGAT
CACTAAAGCA
TCTCTTGCTT
TCAAGGGATC
ACTTGATATT
ACAGAAAACC
AGCTATCATC
TCTAAAAAAG
TTTCATGTGA
GGTTAATGAG
TATCGTGTTG
GATGTTGATG
TTGAACCCTC
CACGACACCA
TGGGATCCTG
CAAAGGCCTA
CCTGTTTGTC
CCTGTTACTG
ACAATACGTC
TATGGAACCC
ATAAAAGGGA
TCAACTGGGA
ATTGGTTGAA
AAGATTACGA
AAACCACAGC
GTGGGACAAC
CCTCTTCAGA
GAGCTCGTCC
CGATGTATAT
CTCGGGACTA
ATGAGAGAAC
TCTTGGTGGG
GACAGAAGGG
AATATGGGTT
TGGTAAACTT
CGTCACAACA
TTGTATTTGG
GCAGAAATCT
CACCCAACTT
ATGGTATTAG
AGGAGCTGTT
GCATCCTTCG
ATGTTGTGAG
AGCTTCTGGA
TTGTTACCTG
TGAGAGATGC
TTTTGAGAAG
GAAGTAGAAG
AGGATTTATC
AAAAGGAAAA
TTGATGATGG
CTGATGGGTG
CATGGTTGCA
GAAGATCTTA
TTGGGAAAAT
AGTGACAGCC
GCACATTCCG
GGAAGCGGTT
TTACGTAGAT
TCAATTTGTT
ATGACATGAG
TGCAGAAACA
TCTTTCCCTA
GGAAAGAGGG
ATATCTTGGT
CCCCTAGAGT
ACAACACGTA
CGCTGACTAT
TACAAGCAAA
AGTATGGAGT
TGATTTGAAC
TGTAAGCAAC
AACCTTTGAT
TAAAGGACGT
GGTGTTTCGT
ATTGCAAGAA
CCTTCTTTCT
CCTCGGTTTG
AACAGTCAGG
TGAATCTGAG
ACAGTCCTTG
ATAAGACATA
TTACGTGTTT
CTTCCACCTG
AACTATGCGT
TGTGCATCCG
CTTTCGGGTT
CACTTATCCG
AGGTGCTCAG
AGGTTACCCT
CTATAATGAG
AAGAGTTGAG
AGCATGTTAC
TCGCTGTTCA
ACTCAAAAAT
TTGATTAGTT
ATCCTATTCC
CACATCAAAG
ATTCTTGAAG
CCTTGACTGC
CGCCTCGTGC
CAGGAAACGT
TCTGCTACGA
GAGAGCTGAG
CTTTTAGGAG
ATGCGTATCT
AAAATGGGTT
GTTAGTCGTT
TTCTGTTTCT
CAAGTATCCT
CAAAAGCACG
AGGGTCAGCT
TACAAGGATG
AGGCAACAAA
TGGTTAGTAA
TATGGTTCTT
TATCAGTTTG
CTCTGGTTAA
AGGGCTTCAG
TCATATATCC
AGGCGGATCG
ATTCGCCCCG
TCTAGGTGGT
CTGCTCCTGG
TGTGCCTCCG
GTTTTCGACG
AGGACACAAA
AGATGGAGGA
TCTTCGTTTT
TCGATGAAGA
GCAAAAACCT
GTTTCCGAGG
AAAACAGCGA
TGTGTTTGAA
GATGGGGAGA
TGGAGGATGG
TGAACTAGAG
GGTGATTTGA
GTTCGTACCG
AGTTTTATCT
AGCAAAACGT
TAGCTGATGT
TGTTGAGGCT
TTGATTGGTG
TTTATTACGT
CGAAGGGGGT
AAGATTGCAG
CTAATCATTT
GATGAAATGG
ATTGGGATTC
ACGTGGAGGA
TGATCCTGAT
GAAGTCGATG
GAACATTGAA
AAATGTTAAT
GTTCCAGAGA
GTGTGCTCAA
GAGCATCGAC
TTTGTTGGTC
TTGAGAGAGC
TCTTAAATAT
GAGTTTAAAG
AGAAAGGTCT
TCTTGTTGAA
GCTATAACAC
ATGCTTCAAG
ACCATCTTCA
GGTGTTTCGT
GTTACCAGAG
ATTGGAATTT
GTTGGTGACG
CGGTCTTGGA
TCATCTCATC
ACAAGACATC
TATTTTTCAC
ATACACAAGC
CTTCCTCCTG
GTCGGTTAAC
AGATCTTCGA
GCTGCAGCGG
TTAACAACGA
GAATTTTGCT
CGCGTTGCGG
TTAAACATAA
ACTCCACTTG
TACCTTCGTC
ACGGTTCAAG
CGCCCTCGAA
AAACAGATTC
GGGAATTTGT
GAAGGAGGTT
CAAACCGAGT
CATCGAAACC
GGGGTTTAAC
TCTTTTGGTT
TGGGAGACTG
CAAAGCACCA
AAAGTTCTTG
GAGACATTGT
TGAATCTATT
GCAGGTGCTA
TTTTTCTTGA
TAGTGGAAAA
GATACAACTG
CTGCTTGGAA
GGTTTTTCAA
CCTTTGCTTC
AGCCCATGGT
GACACCAGAG
ACACTTCCAA
TGCATCCGGT
GCGAGAGCTA
CAAGAGCGGT
GCCAGCAACA
AGCAGAAGGG
TTAGAATACA
AAGCGAGTAG
GAGTGGTAAC
ACAGCGACTG
TGGAAGTTTT
CATCGACGGT
GTTCAAGTTG
GAGTAGCGCA
AAACCCGCAG
AAGAAAATGG
CTCAAAAGCT
AGCTGCGAGG
AACGCACTTG
CAGCTTTGAA
AGAGAAAGAA
ATAGCAGAAT
CAAAGGAGAA
GCATATCAAC
AACGGTAATG
CGGGAGAGGA
TCAAGGCGAG
AATGAGAATG
GGAACAAGAA
GAATGGGCAT
CAGCCGTTTA
CGAGACAAAC
GTTGAATGAT
ATTTGTTTGA
GGAAGAATTG
GCCAATGCCT
TCTTACAGAT
GTGTGAAAGA
AGGAGGACCG
GCTCATGCAA
AGAGATTTAC
GTTTGGGGTA
AGAGTTAATA
CGAGCGATAG
AGGATGGACC
GATGAGTGTA
TTGGCGAGCC
AATGCCGAGT
GTTAAGAAAG
CTAAGGATTC
AGCTGCGGTT
CTTCTACTTG
AGCTTTTAAA
TAAAACTTTT
TCTGGTGGTT
CTGGTTACCC
ATACGACGTC
CCAGACTACG
CTTGAGCGGC
CGCTTAATTA
AGGCCTTGAA
TCGAGAATTT
ATACTTAGAT
AAGTATGTAC
TTACAGGTAT
ATTTCTATGA
GATACTGATG
TATACATGCA
TGATAATATT
TAAACGGTTA
TTAGTGCCGA
TTGTCTTGTG
CGATAATGAC
GTTCCTATCA
AAGCAATACA
CTTACCACCT
ATTACATGGG
CCAAGAAAAT
ATTTTCGAAC
TTGTTTAGAA
TATTAGCACA
GAGTATATGA
TGATATCCGT
TAGATTATGC
ATGATTCATT
CCTACAACTT
TTTCGTAGCA
TAAGGATTAA
TTACTTGGAT
GCCAATAAAA
AAAAAAAACA
TCGAGAAAAT
TTCAGCATGC
TCAGAAACAA
TTGCAGTGTA
TCAAAGTAAA
AAAAAGATTT
TCACTACATG
TTCCTTTTGA
AGAAAGAAAA
TCATGGAACA
TTAGATTTAC
AAAAATTTAA
CCACCGCTGA
TTAACGATTA
GACCGTTAAG
CGCACAACAG
GTTATTAGTA
CAGAGAAAGC
ATTCTGTGGT
GTTGCCCCGG
ACTTTCTTTT
GCGACATAGG
TAAATCGAAT
ACCATCATAC
TATCTTTTCC
AATGACTCCC
TAAAGAAAGA
CTCTTCTTCG
ATGTTGTATA
CGTTGGAGCA
TAGGGCAAGA
ATTGTGGCTT
GAGATCATCC
TTTTGTTGTT
TCCGGGTGTA
CAATATGGAC
TTCCTCTTTT
CTGGCAACCA
AACCCATACA
TCGGGATTCC
TATAATACCT
TCGTTGGTCT
CCCTAACATG
TAGGTGGCGG
AGGGGAGATA
TACAATAGAA
CAGATACCAG
ACAAGACATA
ATGGGCTAAA
CAAGACTACA
CCAATTACAC
TGCCTCATTG
ATGGTGGTAC
ATAACGAACT
AATACTGTAG
CCCTAGACTT
GATAGCCATC
ATCATATCGA
AGTTTCACTA
CCCTTTTTCC
ATTTGCCATC
TATTGAAGTA
ATAATAGGCG
CATGCAACTT
CTTTTCTTTT
TTTTTCTTTT
CTCTCTCCCC
CGTTGTTGTC
TCACCATATC
CGCAATGACA
AAAAAATGAT
GGAAGACACT
AAAGGAAAAA
ATTAACGACA
AAGACAGCAC
CAACAGATGT
CGTTGTTCCA
GAGCTGATGA
GGGGTATCTC
GAAGCACACG
AAACTTTTTC
CTTCCTTCAT
TCACGCACAC
TACTCTCTAA
TGAGCAACGG
TATACGGCCT
TCCTTCCAGT
TACTTGAATT
TGAAATAAAA
AAAAGTTTGC
TGTCTTGCTA
TCAAGTATAA
ATAGACCTGC
AATTATTAAT
CTTTTGTTTC
CTCGTCATTG
TTCTCGTTCC
CTTTCTTCCT
TGTTTCTTTT
TCTGCACAAT
ATTTCAAGCT
ATACCAAGCA
TACAATCAAG
GAATTCCGGA
TCCGCCACCA
TGCCTCAATC
CTGGGAAGAA
CTGGCCGCTG
ATAAGCGCGC
CCGCCTCGCA
AAAACCATCC
CTGATGAATG
GAAAGTCCAG
ACGCTGCCTG
CGGAAGACAG
CGTTATTGAT
TTCCCAAAGA
AATCGGGGAT
CCTTTCAGAG
GCCGAACTGA
AGATCACAGA
GGCTTCCGCT
GCGGATCTTG
TGTCCAAGCT
GGCGGCCGGA
GAGTTGACCT
CGGTGGAAGT
TACGCTAGCA
TTCTGTAAAC
GGGCAGCAAT
CGCCCAGCAG
TTAACAAACT
GCGCCCACGA
GTTCTTCCCT
GACGCCGCTC
TCGCGCAGGC
AAGGGAACTC
GATGAATACT
ACGCAAAGCA
CAAGAGACCC
GTTGGTCCAC
TCCATGGCCT
CCCCATCTCT
CTCAAAGACC
AGCTTCGAGT
CAAGGGCTAC
GAAACATCAA
TGGGCTACAT
CTCATGGCTA
AACAAGTACG
ACGAAGGGGA
CTCGGTTCTG
ACAACCATGC
TCCGCAAAGC
CGGTGCCGTC
TTCTACGTCA
AGACCTCTGT
CCCGCAGACC
CTGATGGTCT
GCGAGACAGT
CAACAACATC
ATCGGGCGCA
CCGTCAACCC
ACGCAACAAG
AACTGGTCGT
GCGGCGGCAG
TTCTGGTGGT
GAGGGTGCGA
TCGTTGGGAT
TCGTGGTGGC
GTCATCGGTG
TAGGAACGGA
TATCGGTGGC
TCGATTCGAG
TGCCGGCCGC
GTTCAACTTC
CTGTACGGTC
TAAGGCCGAG
TCATGGGCGG
CTGCCGTATG
CAAAGATGGC
GAACAGCATG
GAGGGTCAGG
AGACGGTGCA
CAGCGTTGTC
GGGCCGATTA
CGCACTCTGT
TGAGGACCTC
CGCCTCTTCA
CCAAATCCGT
CCTCGGTCAG
GAGCCATGGA
AATACGACTC
CAAGGTCATC
CCCATGCCCT
GGCGCCAGTC
CGAGTCGGAC
ATTATTGCCT
CCAAGATCAA
GAACGGCGGG
CTCAATATCG
GCTACTACAA
CTTCGACGGC
AATGTCCTTC
CACACCCTCC
TATCCTGCGC
GGCGTGGAAA
CTACCGTCGC
CGCACTCGCC
AAAGCCGGTC
ACACCGTGAC
CCCGTGGACG
CCATACAAGC
ACGATTTCGG
CCACGATCTC
ATCTCCCATA
TCTACGCGGC
TGACGGCAGC
GCCGACGTAA
TGCGCGATAT
CAGTGCATCC
GGCGAGCCGG
CGATTCCAAA
TATCAAAGAC
CTACTGAACC
CGAACATCAA
AGCTGTTAAC
ATGAACGAGC
TCTGGGACAC
GCATCTCCAG
AAGTGGAATT
ACCAGATGGA
GTACCTTGAG
AAATGGCGGG
AGGCTGAAGA
AAAGGCCGGG
AAGGAACTGG
ACGCCATCAT
CGCGCCGATT
ACGCCTACCG
CTGCGGTACG
GCATGACCAG
TTCCGGTACT
ATGGGTATGC
CTCTGTGATC
AACCTGCTGG
ATTTCACGAG
CGTGGTTGTT
CCGGTTACCT
TTGCGGATAA
GAACATCGAT
AAGAAGAATG
AGAGTTTCAA
GGCGGTTAGT
GAGCTTGATG
CCCTCGTGCA
GGAAGAGTAT
GATCCGGAGG
CGTACCATGG
GGCACCGGTT
GCAGTGCAGG
TTATCGGACG
GAGACTCAGT
GAAGAGAGGA
CGTTGGCGAT
TGCAGAGGAA
GTGGGGAAGT
TGCTGGGAAA
TGTGGTGACT
CCATAGCCCG
GGGGGGGCTC
GATCCCCTCG
CGAGTTGGTT
CAGCTGCTGC
CTGAGGCTGG
ACGACCTCGC
GGAGTTCTAC
CGGCAGTGCA
AATCCGTCGG
CATCCAGGAA
ACCAGCAGCG
GCTATCCGCG
CATCCATGCC
CCCGAACTGC
AGGAGTGGGG
AGGCACGATG
GCCGCTTTGG
TCGATCTAGA
TTACGTGGAA
GAAAGGTAGT
AAAAGTAGTA
GTATAAGTAG
TAAAAAGAGG
TAAAAAGAGA
AAACCGGCTA
CATACTAGAG
AAGCACGTAC
ACAAAAACTC
ATAGGCACTT
CATCATACGA
CAGTTTCTTG
ATGCATTATA
ATAGTGTATT
AGATATTTTC
AGAAATATGC
ATAGAACCTC
CTCTTGCCTT
TACTTTTTAT
ACATAGAACA
TTGGCAGATT
TACTTACACT
ACTTTGTTTC
TACGCCATTT
CTTTTGTTTT
CAACACTTAG
ACAAGTTGTT
GAGAACCGGA
CTACTAAAAA
GCAATGTTCC
CACTGAAAAT
CATGTACCTG
CAGGATAATA
ACCCCCTAAT
TCTGCATCGA
TCCAGTATGT
TTTTTTTTCT
CTACTCATTT
TTACCTGAAG
ATAGAGCTTC
TAAAACAAAA
AAAATCAGTG
ATTACATGCA
TATTGTGTGT
TCTAGTAACC
AAAGGAAAGG
AACAGATAGA
TAAAATTCCG
AGACTGTCAA
ATTAGGTTTT
TTTCTTTTTT
TTTGGCGGGA
GTCAGTGGGC
CGAAATATGT
TCTTGGCCTA
GAACTTAATC
TGGTTTGATC
ATGCCAATAC
TTGCCTGAGT
GCCCGACTTT
TTGCCCACCC
TCTTGCCTTC
TGTCATCCTT
CAAAACCCAC
CTGTTTTCCA
GCCGTATCTT
CGCTCGCATC
TACACATACT
GTGCCATATC
TTGTGTGTAG
CCGGACGTGA
CTATGACCAA
AAACAAACAA
GGAGAACTGT
TCGCCGATTT
GTAACACTCC
TGCATCCATC
CAAGTGGGTA
TGCGCTATGC
AATGTTAAGC
TAGGTCAGGT
CAGACCAGGT
CCAAGGACAG
CAACTTGACT
GTATGCAACC
TTTACCATCT
TTGCACAGAA
CATACTTGTA
GCTAGCTAGT
TACACTTATG
GACCGAAAAG
GCACCCCACC
ATGTCTGTCC
GGCTTTAGAG
TACGGCCGCA
GACCGCTGAT
TTGCCTTGCC
AAGCAGTAGT
CACAATGCAT
CGCATGAGCA
CACGGGCACG
GGCACGGGCA
CAGGAACCAT
TGGCAAAAAT
ACCAGATACA
CTATACCGAC
GTATATCAAG
CCCAAGTTTA
AAATTCCTAA
ATTTCCGCGG
CTACTTTTCA
ATTCCCTATA
GTGAGTCGTA
TTAAATTCGT
AATCATGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGCGCCCTGT
AGCGGCGCAT
TAAGCGCGGC
GGGTGTGGTG
GTTACGCGCA
GCGTGACCGC
TACACTTGCC
AGCGCCCTAG
CGCCCGCTCC
TTTCGCTTTC
TTCCCTTCCT
TTCTCGCCAC
GTTCGCCGGC
TTTCCCCGTC
AAGCTCTAAA
TCGGGGGCTC
CCTTTAGGGT
TCCGATTTAG
TGCTTTACGG
CACCTCGACC
CCAAAAAACT
TGATTAGGGT
GATGGTTCAC
GTAGTGGGCC
ATCGCCCTGA
TAGACGGTTT
TTCGCCCTTT
GACGTTGGAG
TCCACGTTCT
TTAATAGTGG
ACTCTTGTTC
CAAACTGGAA
CAACACTCAA
CCCTATCTCG
GTCTATTCTT
TTGATTTATA
AGGGATTTTG
CCGATTTCGG
CCTATTGGTT
AAAAAATGAG
CTGATTTAAC
AAAAATTTAA
CGCGAATTTT
AACAAAATAT
TAACGCTTAC
AATTTCCATT
CGCCATTCAG
GCTGCGCAAC
TGTTGGGAAG
GGCGATCGGT
GCGGGCCTCT
TCGCTATTAC
GCCAGCTGGC
GAAAGGGGGA
TGTGCTGCAA
GGCGATTAAG
TTGGGTAACG
CCAGGGTTTT
CCCAGTCACG
ACGTTGTAAA
ACGACGGCCA
GTGCCAAGCT
CCCGCGGGGA
TCGACTCATA
AAATAGTAAC
CTTCTAATGC
GTATCTATTG
ACTACCAACC
ATTAGTGTGG
TTGCAGAAGG
CGGAATTCTC
CCTTCTTCGA
ATTCAGCTTG
CTTTTTCATT
TTTTATTTTC
CATTTTTCAG
TTTTTGTTTG
TGTCGAATTT
AGCCAGTTGC
TTCTCCAAGA
TGAAAAAAAC
CCCTGCGCAG
TTTCTGTGCT
GCAAGATCCT
AATCGACTTT
TCCACCCCCC
ACAAAAGTAA
ATGTTCTTTT
GTTACATTCG
CGTGGGTAGC
TAGCTCCCCG
AATCTTCAAA
GGACTTAGGG
ACTGCACTAC
ATCAGAGTGT
GTTCACCTGG
TTTGCTGCCT
GGTTTGAAAG
AAAAGAGCAG
GGAACTCGCG
GGTTCCCGGC
GAATAATCAT
GCGATAGTCC
TTTGGCCTTC
CAAGTCGCAT
GTAGAGTAGA
CAACAGACAG
GGAGGGCAGG
AAGGATCTTT
CACTGAGATC
CTGTATCTTG
TTGGGTAAGT
CGGATGAAAG
GGGAATCGTA
TGAGATTGGA
GAGGATGCGG
AAGAGGTAAC
GCCTTTTGTT
AACTTGTTTA
ATTATTATGG
GGCAGGCGAG
AGGGGGAGGA
ATGTATGTGT
GTGAGGCGGG
CGAGACGGAG
CCATCCAGGC
CAGGTAGAAA
TAGAGAAAGC
CGAATGTTAG
ACAATATGGC
AGCGTAGTAG
AGTAGGTAGG
TAGGCAAGTA
CTGCTAGCAA
AGAGGAGAAG
GGTAAGCTCA
CTCTTCGCAT
TCCACACCGT
TAGTGTGTCA
GTTTGAACAA
AAAAACAATC
ATCATACCAA
TTGATGGACT
GTGGACTGGC
TTTTGGAACG
GCTTTTCGGA
CTGCGATTAT
TCGTGAGGAA
TCAAGGTAGG
AATTTGGTCA
TATTTACGGA
CAACAGTGGG
TGATTCCCAT
ATGGAGTAGG
AAAACGAGAT
CATGGTATCC
TCAGATATGT
TGCGGAATTC
TGTTCACCGC
AAAGTTCAGG
GTGCTCTGGT
GGGTTTCGGT
TGGTCTTTGC
TTTGCTTCTC
CCTTGTCTTG
CATGTTAATA
ATAGCCTAGC
CTGTGAGCCG
AAACTTAGGG
TAGGCTTAGT
GTTGGAACGT
ACATATGTAT
CACGTTGACT
TGGTTTAACC
AGGCGACCTG
GTAGCCAGCC
ATACCCACAC
ACGTTTTTTG
TATCTTCAGT
ATAGTTGTGA
AAAGTGTAGC
GGAAATTTGT
GGTCCGAGCA
ACAGCGTCTT
TTTCTAGTAG
TGCGGTCGGT
TACTTGGTTG
ACATTGGTAT
TTGGACTTTG
TTGCTACACC
ATTCACTACT
TGAAGTCGAG
TGTGAAGGGT
ATGATTTCTA
GTGGTGAACA
CCTTTAGTTA
CGTAATGTTT
TCATTGCTGT
TTTACTTGAG
ATTTCGATTG
AGAAAAAGGT
ATTTAATAGC
TCGAATCAAT
GTGAGAACAG
AGAGAAGATG
TTCTTCCCTA
ACTCGAAAGG
TATATGAGGC
TTGTGTTTCT
TAGGAGAATT
ATTATTCTTT
TGTTATGTTG
CGCTTGTAGT
TGGAAAAGGT
GAAGAGACAA
AAGCTGGAAT
TGTGAGCGGA
TAACAAGCTC
AACACTTGAA
ATTTAGGAAA
GAGCAGAATT
TGGCAAAAAA
AATAAAAAAA
AAATAAACAC
ACATACTCAT
CGAG
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