名称: | pLV3-U6-Pcyt1b(mouse)-sgRNA1-Cas9-Blast |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | LentiCRISPR v2-Blast |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
AGGTACCGAG
GGCCTATTTC
CCATGATTCC
TTCATATTTG
CATATACGAT
ACAAGGCTGT
TAGAGAGATA
ATTAGAATTA
ATTTGACTGT
AAACACAAAG
ATATTAGTAC
AAAATACGTG
ACGTAGAAAG
TAATAATTTC
TTGGGTAGTT
TGCAGTTTTA
AAATTATGTT
TTAAAATGGA
CTATCATATG
CTTACCGTAA
CTTGAAAGTA
TTTCGATTTC
TTGGCTTTAT
ATATCTTGTG
GAAAGGACGA
AACACCGTCT
CAGACACTGT
CGATACGGTT
TTAGAGCTAG
AAATAGCAAG
TTAAAATAAG
GCTAGTCCGT
TATCAACTTG
AAAAAGTGGC
ACCGAGTCGG
TGCTTTTTTG
AATTCGCTAG
CTAGGTCTTG
AAAGGAGTGG
GAATTGGCTC
CGGTGCCCGT
CAGTGGGCAG
AGCGCACATC
GCCCACAGTC
CCCGAGAAGT
TGGGGGGAGG
GGTCGGCAAT
TGATCCGGTG
CCTAGAGAAG
GTGGCGCGGG
GTAAACTGGG
AAAGTGATGT
CGTGTACTGG
CTCCGCCTTT
TTCCCGAGGG
TGGGGGAGAA
CCGTATATAA
GTGCAGTAGT
CGCCGTGAAC
GTTCTTTTTC
GCAACGGGTT
TGCCGCCAGA
ACACAGGACC
GGTTCTAGAG
CGCTGCCACC
ATGGACAAGA
AGTACAGCAT
CGGCCTGGAC
ATCGGCACCA
ACTCTGTGGG
CTGGGCCGTG
ATCACCGACG
AGTACAAGGT
GCCCAGCAAG
AAATTCAAGG
TGCTGGGCAA
CACCGACCGG
CACAGCATCA
AGAAGAACCT
GATCGGAGCC
CTGCTGTTCG
ACAGCGGCGA
AACAGCCGAG
GCCACCCGGC
TGAAGAGAAC
CGCCAGAAGA
AGATACACCA
GACGGAAGAA
CCGGATCTGC
TATCTGCAAG
AGATCTTCAG
CAACGAGATG
GCCAAGGTGG
ACGACAGCTT
CTTCCACAGA
CTGGAAGAGT
CCTTCCTGGT
GGAAGAGGAT
AAGAAGCACG
AGCGGCACCC
CATCTTCGGC
AACATCGTGG
ACGAGGTGGC
CTACCACGAG
AAGTACCCCA
CCATCTACCA
CCTGAGAAAG
AAACTGGTGG
ACAGCACCGA
CAAGGCCGAC
CTGCGGCTGA
TCTATCTGGC
CCTGGCCCAC
ATGATCAAGT
TCCGGGGCCA
CTTCCTGATC
GAGGGCGACC
TGAACCCCGA
CAACAGCGAC
GTGGACAAGC
TGTTCATCCA
GCTGGTGCAG
ACCTACAACC
AGCTGTTCGA
GGAAAACCCC
ATCAACGCCA
GCGGCGTGGA
CGCCAAGGCC
ATCCTGTCTG
CCAGACTGAG
CAAGAGCAGA
CGGCTGGAAA
ATCTGATCGC
CCAGCTGCCC
GGCGAGAAGA
AGAATGGCCT
GTTCGGAAAC
CTGATTGCCC
TGAGCCTGGG
CCTGACCCCC
AACTTCAAGA
GCAACTTCGA
CCTGGCCGAG
GATGCCAAAC
TGCAGCTGAG
CAAGGACACC
TACGACGACG
ACCTGGACAA
CCTGCTGGCC
CAGATCGGCG
ACCAGTACGC
CGACCTGTTT
CTGGCCGCCA
AGAACCTGTC
CGACGCCATC
CTGCTGAGCG
ACATCCTGAG
AGTGAACACC
GAGATCACCA
AGGCCCCCCT
GAGCGCCTCT
ATGATCAAGA
GATACGACGA
GCACCACCAG
GACCTGACCC
TGCTGAAAGC
TCTCGTGCGG
CAGCAGCTGC
CTGAGAAGTA
CAAAGAGATT
TTCTTCGACC
AGAGCAAGAA
CGGCTACGCC
GGCTACATTG
ACGGCGGAGC
CAGCCAGGAA
GAGTTCTACA
AGTTCATCAA
GCCCATCCTG
GAAAAGATGG
ACGGCACCGA
GGAACTGCTC
GTGAAGCTGA
ACAGAGAGGA
CCTGCTGCGG
AAGCAGCGGA
CCTTCGACAA
CGGCAGCATC
CCCCACCAGA
TCCACCTGGG
AGAGCTGCAC
GCCATTCTGC
GGCGGCAGGA
AGATTTTTAC
CCATTCCTGA
AGGACAACCG
GGAAAAGATC
GAGAAGATCC
TGACCTTCCG
CATCCCCTAC
TACGTGGGCC
CTCTGGCCAG
GGGAAACAGC
AGATTCGCCT
GGATGACCAG
AAAGAGCGAG
GAAACCATCA
CCCCCTGGAA
CTTCGAGGAA
GTGGTGGACA
AGGGCGCTTC
CGCCCAGAGC
TTCATCGAGC
GGATGACCAA
CTTCGATAAG
AACCTGCCCA
ACGAGAAGGT
GCTGCCCAAG
CACAGCCTGC
TGTACGAGTA
CTTCACCGTG
TATAACGAGC
TGACCAAAGT
GAAATACGTG
ACCGAGGGAA
TGAGAAAGCC
CGCCTTCCTG
AGCGGCGAGC
AGAAAAAGGC
CATCGTGGAC
CTGCTGTTCA
AGACCAACCG
GAAAGTGACC
GTGAAGCAGC
TGAAAGAGGA
CTACTTCAAG
AAAATCGAGT
GCTTCGACTC
CGTGGAAATC
TCCGGCGTGG
AAGATCGGTT
CAACGCCTCC
CTGGGCACAT
ACCACGATCT
GCTGAAAATT
ATCAAGGACA
AGGACTTCCT
GGACAATGAG
GAAAACGAGG
ACATTCTGGA
AGATATCGTG
CTGACCCTGA
CACTGTTTGA
GGACAGAGAG
ATGATCGAGG
AACGGCTGAA
AACCTATGCC
CACCTGTTCG
ACGACAAAGT
GATGAAGCAG
CTGAAGCGGC
GGAGATACAC
CGGCTGGGGC
AGGCTGAGCC
GGAAGCTGAT
CAACGGCATC
CGGGACAAGC
AGTCCGGCAA
GACAATCCTG
GATTTCCTGA
AGTCCGACGG
CTTCGCCAAC
AGAAACTTCA
TGCAGCTGAT
CCACGACGAC
AGCCTGACCT
TTAAAGAGGA
CATCCAGAAA
GCCCAGGTGT
CCGGCCAGGG
CGATAGCCTG
CACGAGCACA
TTGCCAATCT
GGCCGGCAGC
CCCGCCATTA
AGAAGGGCAT
CCTGCAGACA
GTGAAGGTGG
TGGACGAGCT
CGTGAAAGTG
ATGGGCCGGC
ACAAGCCCGA
GAACATCGTG
ATCGAAATGG
CCAGAGAGAA
CCAGACCACC
CAGAAGGGAC
AGAAGAACAG
CCGCGAGAGA
ATGAAGCGGA
TCGAAGAGGG
CATCAAAGAG
CTGGGCAGCC
AGATCCTGAA
AGAACACCCC
GTGGAAAACA
CCCAGCTGCA
GAACGAGAAG
CTGTACCTGT
ACTACCTGCA
GAATGGGCGG
GATATGTACG
TGGACCAGGA
ACTGGACATC
AACCGGCTGT
CCGACTACGA
TGTGGACCAT
ATCGTGCCTC
AGAGCTTTCT
GAAGGACGAC
TCCATCGACA
ACAAGGTGCT
GACCAGAAGC
GACAAGAACC
GGGGCAAGAG
CGACAACGTG
CCCTCCGAAG
AGGTCGTGAA
GAAGATGAAG
AACTACTGGC
GGCAGCTGCT
GAACGCCAAG
CTGATTACCC
AGAGAAAGTT
CGACAATCTG
ACCAAGGCCG
AGAGAGGCGG
CCTGAGCGAA
CTGGATAAGG
CCGGCTTCAT
CAAGAGACAG
CTGGTGGAAA
CCCGGCAGAT
CACAAAGCAC
GTGGCACAGA
TCCTGGACTC
CCGGATGAAC
ACTAAGTACG
ACGAGAATGA
CAAGCTGATC
CGGGAAGTGA
AAGTGATCAC
CCTGAAGTCC
AAGCTGGTGT
CCGATTTCCG
GAAGGATTTC
CAGTTTTACA
AAGTGCGCGA
GATCAACAAC
TACCACCACG
CCCACGACGC
CTACCTGAAC
GCCGTCGTGG
GAACCGCCCT
GATCAAAAAG
TACCCTAAGC
TGGAAAGCGA
GTTCGTGTAC
GGCGACTACA
AGGTGTACGA
CGTGCGGAAG
ATGATCGCCA
AGAGCGAGCA
GGAAATCGGC
AAGGCTACCG
CCAAGTACTT
CTTCTACAGC
AACATCATGA
ACTTTTTCAA
GACCGAGATT
ACCCTGGCCA
ACGGCGAGAT
CCGGAAGCGG
CCTCTGATCG
AGACAAACGG
CGAAACCGGG
GAGATCGTGT
GGGATAAGGG
CCGGGATTTT
GCCACCGTGC
GGAAAGTGCT
GAGCATGCCC
CAAGTGAATA
TCGTGAAAAA
GACCGAGGTG
CAGACAGGCG
GCTTCAGCAA
AGAGTCTATC
CTGCCCAAGA
GGAACAGCGA
TAAGCTGATC
GCCAGAAAGA
AGGACTGGGA
CCCTAAGAAG
TACGGCGGCT
TCGACAGCCC
CACCGTGGCC
TATTCTGTGC
TGGTGGTGGC
CAAAGTGGAA
AAGGGCAAGT
CCAAGAAACT
GAAGAGTGTG
AAAGAGCTGC
TGGGGATCAC
CATCATGGAA
AGAAGCAGCT
TCGAGAAGAA
TCCCATCGAC
TTTCTGGAAG
CCAAGGGCTA
CAAAGAAGTG
AAAAAGGACC
TGATCATCAA
GCTGCCTAAG
TACTCCCTGT
TCGAGCTGGA
AAACGGCCGG
AAGAGAATGC
TGGCCTCTGC
CGGCGAACTG
CAGAAGGGAA
ACGAACTGGC
CCTGCCCTCC
AAATATGTGA
ACTTCCTGTA
CCTGGCCAGC
CACTATGAGA
AGCTGAAGGG
CTCCCCCGAG
GATAATGAGC
AGAAACAGCT
GTTTGTGGAA
CAGCACAAGC
ACTACCTGGA
CGAGATCATC
GAGCAGATCA
GCGAGTTCTC
CAAGAGAGTG
ATCCTGGCCG
ACGCTAATCT
GGACAAAGTG
CTGTCCGCCT
ACAACAAGCA
CCGGGATAAG
CCCATCAGAG
AGCAGGCCGA
GAATATCATC
CACCTGTTTA
CCCTGACCAA
TCTGGGAGCC
CCTGCCGCCT
TCAAGTACTT
TGACACCACC
ATCGACCGGA
AGAGGTACAC
CAGCACCAAA
GAGGTGCTGG
ACGCCACCCT
GATCCACCAG
AGCATCACCG
GCCTGTACGA
GACACGGATC
GACCTGTCTC
AGCTGGGAGG
CGACAAGCGA
CCTGCCGCCA
CAAAGAAGGC
TGGACAGGCT
AAGAAGAAGA
AAGATTACAA
AGACGATGAC
GATAAGGGAT
CCGGCGCAAC
AAACTTCTCT
CTGCTGAAAC
AAGCCGGAGA
TGTCGAAGAG
AATCCTGGAC
CGATGGCCAA
GCCTTTGTCT
CAAGAAGAAT
CCACCCTCAT
TGAAAGAGCA
ACGGCTACAA
TCAACAGCAT
CCCCATCTCT
GAAGACTACA
GCGTCGCCAG
CGCAGCTCTC
TCTAGCGACG
GCCGCATCTT
CACTGGTGTC
AATGTATATC
ATTTTACTGG
GGGACCTTGT
GCAGAACTCG
TGGTGCTGGG
CACTGCTGCT
GCTGCGGCAG
CTGGCAACCT
GACTTGTATC
GTCGCGATCG
GAAATGAGAA
CAGGGGCATC
TTGAGCCCCT
GCGGACGGTG
CCGACAGGTG
CTTCTCGATC
TGCATCCTGG
GATCAAAGCC
ATAGTGAAGG
ACAGTGATGG
ACAGCCGACG
GCAGTTGGGA
TTCGTGAATT
GCTGCCCTCT
GGTTATGTGT
GGGAGGGCTA
AGAATTCGAT
ATCAAGCTTA
TCGGTAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCGTCGAC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
GGCCCGTTTA
AACCCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGACAA
TAGCAGGCAT
GCTGGGGATG
CGGTGGGCTC
TATGGCTTCT
GAGGCGGAAA
GAACCAGCTG
GGGCTCTAGG
GGGTATCCCC
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTA
ATTCTGTGGA
ATGTGTGTCA
GTTAGGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCAGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAT
AGTCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CTGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGCTTTTGCA
AAAAGCTCCC
GGGAGCTTGT
ATATCCATTT
TCGGATCTGA
TCAGCACGTG
TTGACAATTA
ATCATCGGCA
TAGTATATCG
GCATAGTATA
ATACGACAAG
GTGAGGAACT
AAACCATGGC
CAAGTTGACC
AGTGCCGTTC
CGGTGCTCAC
CGCGCGCGAC
GTCGCCGGAG
CGGTCGAGTT
CTGGACCGAC
CGGCTCGGGT
TCTCCCGGGA
CTTCGTGGAG
GACGACTTCG
CCGGTGTGGT
CCGGGACGAC
GTGACCCTGT
TCATCAGCGC
GGTCCAGGAC
CAGGTGGTGC
CGGACAACAC
CCTGGCCTGG
GTGTGGGTGC
GCGGCCTGGA
CGAGCTGTAC
GCCGAGTGGT
CGGAGGTCGT
GTCCACGAAC
TTCCGGGACG
CCTCCGGGCC
GGCCATGACC
GAGATCGGCG
AGCAGCCGTG
GGGGCGGGAG
TTCGCCCTGC
GCGACCCGGC
CGGCAACTGC
GTGCACTTCG
TGGCCGAGGA
GCAGGACTGA
CACGTGCTAC
GAGATTTCGA
TTCCACCGCC
GCCTTCTATG
AAAGGTTGGG
CTTCGGAATC
GTTTTCCGGG
ACGCCGGCTG
GATGATCCTC
CAGCGCGGGG
ATCTCATGCT
GGAGTTCTTC
GCCCACCCCA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
TATACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGAC