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pLV3-U6-DDX46(cattle)-shRNA3-CopGFP-Puro

产品货号:P67767

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV3-U6-DDX46(cattle)-shRNA3-CopGFP-Puro
别称: -
基因长:
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 干扰沉默
片段类型: shRNA
片段物种:
原核抗性: Amp
筛选标记: Puro
荧光标记: 绿色
启动子: U6
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体: pLV3-U6-MCS-shRNA-CopGFP-Puro
正向引物: U6-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ACGCGTGTAG

TCTTATGCAA

TACTCTTGTA

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

61

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

121

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

181

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

GTGCCTAGCT

CGATACAATA

AACGGGTCTC

241

TCTGGTTAGA

CCAGATCTGA

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

301

AGCCTCAATA

AAGCTTGCCT

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

361

CTGGTAACTA

GAGATCCCTC

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

421

CCCGAACAGG

GACCTGAAAG

CGAAAGGGAA

ACCAGAGCTC

TCTCGACGCA

GGACTCGGCT

481

TGCTGAAGCG

CGCACGGCAA

GAGGCGAGGG

GCGGCGACTG

GTGAGTACGC

CAAAAATTTT

541

GACTAGCGGA

GGCTAGAAGG

AGAGAGATGG

GTGCGAGAGC

GTCAGTATTA

AGCGGGGGAG

601

AATTAGATCG

CGATGGGAAA

AAATTCGGTT

AAGGCCAGGG

GGAAAGAAAA

AATATAAATT

661

AAAACATATA

GTATGGGCAA

GCAGGGAGCT

AGAACGATTC

GCAGTTAATC

CTGGCCTGTT

721

AGAAACATCA

GAAGGCTGTA

GACAAATACT

GGGACAGCTA

CAACCATCCC

TTCAGACAGG

781

ATCAGAAGAA

CTTAGATCAT

TATATAATAC

AGTAGCAACC

CTCTATTGTG

TGCATCAAAG

841

GATAGAGATA

AAAGACACCA

AGGAAGCTTT

AGACAAGATA

GAGGAAGAGC

AAAACAAAAG

901

TAAGACCACC

GCACAGCAAG

CGGCCACTGA

TCTTCAGACC

TGGAGGAGGA

GATATGAGGG

961

ACAATTGGAG

AAGTGAATTA

TATAAATATA

AAGTAGTAAA

AATTGAACCA

TTAGGAGTAG

1021

CACCCACCAA

GGCAAAGAGA

AGAGTGGTGC

AGAGAGAAAA

AAGAGCAGTG

GGAATAGGAG

1081

CTTTGTTCCT

TGGGTTCTTG

GGAGCAGCAG

GAAGCACTAT

GGGCGCAGCC

TCAATGACGC

1141

TGACGGTACA

GGCCAGACAA

TTATTGTCTG

GTATAGTGCA

GCAGCAGAAC

AATTTGCTGA

1201

GGGCTATTGA

GGCGCAACAG

CATCTGTTGC

AACTCACAGT

CTGGGGCATC

AAGCAGCTCC

1261

AGGCAAGAAT

CCTGGCTGTG

GAAAGATACC

TAAAGGATCA

ACAGCTCCTG

GGGATTTGGG

1321

GTTGCTCTGG

AAAACTCATT

TGCACCACTG

CTGTGCCTTG

GAATGCTAGT

TGGAGTAATA

1381

AATCTCTGGA

ACAGATTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

1441

TTACACAAGC

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

1501

ACAAGAATTA

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

1561

TTGGCTGTGG

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

1621

AGTTTTTGCT

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

1681

TCAGACCCAC

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

1741

TGGAGAGAGA

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGGTT

1801

AACTTTTAAA

AGAAAAGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

1861

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAT

TCAAAATTTT

1921

ATCGATACTA

GTGAGGGCCT

ATTTCCCATG

ATTCCTTCAT

ATTTGCATAT

ACGATACAAG

1981

GCTGTTAGAG

AGATAATTAG

AATTAATTTG

ACTGTAAACA

CAAAGATATT

AGTACAAAAT

2041

ACGTGACGTA

GAAAGTAATA

ATTTCTTGGG

TAGTTTGCAG

TTTTAAAATT

ATGTTTTAAA

2101

ATGGACTATC

ATATGCTTAC

CGTAACTTGA

AAGTATTTCG

ATTTCTTGGC

TTTATATATC

2161

TTGTGGAAAG

GACTCATCGC

TAGCGAATTC

GGGAGCGATT

TCAAAGATCA

TTCAAGAGAT

2221

GATCTTTGAA

ATCGCTCCTT

TTTTGGATCC

GCGGCCGCGA

AGGATCTGCG

ATCGCTCCGG

2281

TGCCCGTCAG

TGGGCAGAGC

GCACATCGCC

CACAGTCCCC

GAGAAGTTGG

GGGGAGGGGT

2341

CGGCAATTGA

ACGGGTGCCT

AGAGAAGGTG

GCGCGGGGTA

AACTGGGAAA

GTGATGTCGT

2401

GTACTGGCTC

CGCCTTTTTC

CCGAGGGTGG

GGGAGAACCG

TATATAAGTG

CAGTAGTCGC

2461

CGTGAACGTT

CTTTTTCGCA

ACGGGTTTGC

CGCCAGAACA

CAGCTGAAGC

TTCGAGGGGC

2521

TCGCATCTCT

CCTTCACGCG

CCCGCCGCCC

TACCTGAGGC

CGCCATCCAC

GCCGGTTGAG

2581

TCGCGTTCTG

CCGCCTCCCG

CCTGTGGTGC

CTCCTGAACT

GCGTCCGCCG

TCTAGGTAAG

2641

TTTAAAGCTC

AGGTCGAGAC

CGGGCCTTTG

TCCGGCGCTC

CCTTGGAGCC

TACCTAGACT

2701

CAGCCGGCTC

TCCACGCTTT

GCCTGACCCT

GCTTGCTCAA

CTCTACGTCT

TTGTTTCGTT

2761

TTCTGTTCTG

CGCCGTTACA

GATCCAAGCT

GTGACCGGCG

CCTACGCTAG

ACGCCACCAT

2821

GGAGAGCGAC

GAGAGCGGCC

TGCCCGCCAT

GGAGATCGAG

TGCCGCATCA

CCGGCACCCT

2881

GAACGGCGTG

GAGTTCGAGC

TGGTGGGCGG

CGGAGAGGGC

ACCCCCAAGC

AGGGCCGCAT

2941

GACCAACAAG

ATGAAGAGCA

CCAAAGGCGC

CCTGACCTTC

AGCCCCTACC

TGCTGAGCCA

3001

CGTGATGGGC

TACGGCTTCT

ACCACTTCGG

CACCTACCCC

AGCGGCTACG

AGAACCCCTT

3061

CCTGCACGCC

ATCAACAACG

GCGGCTACAC

CAACACCCGC

ATCGAGAAGT

ACGAGGACGG

3121

CGGCGTGCTG

CACGTGAGCT

TCAGCTACCG

CTACGAGGCC

GGCCGCGTGA

TCGGCGACTT

3181

CAAGGTGGTG

GGCACCGGCT

TCCCCGAGGA

CAGCGTGATC

TTCACCGACA

AGATCATCCG

3241

CAGCAACGCC

ACCGTGGAGC

ACCTGCACCC

CATGGGCGAT

AACGTGCTGG

TGGGCAGCTT

3301

CGCCCGCACC

TTCAGCCTGC

GCGACGGCGG

CTACTACAGC

TTCGTGGTGG

ACAGCCACAT

3361

GCACTTCAAG

AGCGCCATCC

ACCCCAGCAT

CCTGCAGAAC

GGGGGCCCCA

TGTTCGCCTT

3421

CCGCCGCGTG

GAGGAGCTGC

ACAGCAACAC

CGAGCTGGGC

ATCGTGGAGT

ACCAGCACGC

3481

CTTCAAGACC

CCCATCGCCT

TCGCCAGATC

CCGCGCTCAG

TCGTCCAATT

CTGCCGTGGA

3541

CGGCACCGCC

GGACCCGGCT

CCACCGGATC

TCGCGAGGGC

AGAGGAAGTC

TTCTAACATG

3601

CGGTGACGTG

GAGGAGAATC

CCGGCCCTAT

GACCGAGTAC

AAGCCCACGG

TGCGCCTCGC

3661

CACCCGCGAC

GACGTCCCCA

GGGCCGTACG

CACCCTCGCC

GCCGCGTTCG

CCGACTACCC

3721

CGCCACGCGC

CACACCGTCG

ATCCGGACCG

CCACATCGAG

CGGGTCACCG

AGCTGCAAGA

3781

ACTCTTCCTC

ACGCGCGTCG

GGCTCGACAT

CGGCAAGGTG

TGGGTCGCGG

ACGACGGCGC

3841

CGCGGTGGCG

GTCTGGACCA

CGCCGGAGAG

CGTCGAAGCG

GGGGCGGTGT

TCGCCGAGAT

3901

CGGCCCGCGC

ATGGCCGAGT

TGAGCGGTTC

CCGGCTGGCC

GCGCAGCAAC

AGATGGAAGG

3961

CCTCCTGGCG

CCGCACCGGC

CCAAGGAGCC

CGCGTGGTTC

CTGGCCACCG

TCGGCGTCTC

4021

GCCCGACCAC

CAGGGCAAGG

GTCTGGGCAG

CGCCGTCGTG

CTCCCCGGAG

TGGAGGCGGC

4081

CGAGCGCGCC

GGGGTGCCCG

CCTTCCTGGA

GACCTCCGCG

CCCCGCAACC

TCCCCTTCTA

4141

CGAGCGGCTC

GGCTTCACCG

TCACCGCCGA

CGTCGAGGTG

CCCGAAGGAC

CGCGCACCTG

4201

GTGCATGACC

CGCAAGCCCG

GTGCCTGAAA

TCAACCTCTG

GATTACAAAA

TTTGTGAAAG

4261

ATTGACTGGT

ATTCTTAACT

ATGTTGCTCC

TTTTACGCTA

TGTGGATACG

CTGCTTTAAT

4321

GCCTTTGTAT

CATGCTATTG

CTTCCCGTAT

GGCTTTCATT

TTCTCCTCCT

TGTATAAATC

4381

CTGGTTGCTG

TCTCTTTATG

AGGAGTTGTG

GCCCGTTGTC

AGGCAACGTG

GCGTGGTGTG

4441

CACTGTGTTT

GCTGACGCAA

CCCCCACTGG

TTGGGGCATT

GCCACCACCT

GTCAGCTCCT

4501

TTCCGGGACT

TTCGCTTTCC

CCCTCCCTAT

TGCCACGGCG

GAACTCATCG

CCGCCTGCCT

4561

TGCCCGCTGC

TGGACAGGGG

CTCGGCTGTT

GGGCACTGAC

AATTCCGTGG

TGTTGTCGGG

4621

GAAGCTGACG

TCCTTTCCAT

GGCTGCTCGC

CTGTGTTGCC

ACCTGGATTC

TGCGCGGGAC

4681

GTCCTTCTGC

TACGTCCCTT

CGGCCCTCAA

TCCAGCGGAC

CTTCCTTCCC

GCGGCCTGCT

4741

GCCGGCTCTG

CGGCCTCTTC

CGCGTCTCCG

CCTTCGCCCT

CAGACGAGTC

GGATCTCCCT

4801

TTGGCCGCCT

CCCCGCCTGG

TACCTTTAAG

ACCAATGACT

TACAAGGCAG

CTGTAGATCT

4861

TAGCCACTTT

TTAAAAGAAA

AGGGGGGACT

GGAAGGGCTA

ATTCACTCCC

AACGAAAATA

4921

AGATCTGCTT

TTTGCTTGTA

CTGGGTCTCT

CTGGTTAGAC

CAGATCTGAG

CCTGGGAGCT

4981

CTCTGGCTAA

CTAGGGAACC

CACTGCTTAA

GCCTCAATAA

AGCTTGCCTT

GAGTGCTTCA

5041

AGTAGTGTGT

GCCCGTCTGT

TGTGTGACTC

TGGTAACTAG

AGATCCCTCA

GACCCTTTTA

5101

GTCAGTGTGG

AAAATCTCTA

GCAGTAGTAG

TTCATGTCAT

CTTATTATTC

AGTATTTATA

5161

ACTTGCAAAG

AAATGAATAT

CAGAGAGTGA

GAGGAACTTG

TTTATTGCAG

CTTATAATGG

5221

TTACAAATAA

AGCAATAGCA

TCACAAATTT

CACAAATAAA

GCATTTTTTT

CACTGCATTC

5281

TAGTTGTGGT

TTGTCCAAAC

TCATCAATGT

ATCTTATCAT

GTCTGGCTCT

AGCTATCCCG

5341

CCCCTAACTC

CGCCCAGTTC

CGCCCATTCT

CCGCCCCATG

GCTGACTAAT

TTTTTTTATT

5401

TATGCAGAGG

CCGAGGCCGC

CTCGGCCTCT

GAGCTATTCC

AGAAGTAGTG

AGGAGGCTTT

5461

TTTGGAGGCC

TAGACTTTTG

CAGAGACGGC

CCAAATTCGT

AATCATGGTC

ATAGCTGTTT

5521

CCTGTGTGAA

ATTGTTATCC

GCTCACAATT

CCACACAACA

TACGAGCCGG

AAGCATAAAG

5581

TGTAAAGCCT

GGGGTGCCTA

ATGAGTGAGC

TAACTCACAT

TAATTGCGTT

GCGCTCACTG

5641

CCCGCTTTCC

AGTCGGGAAA

CCTGTCGTGC

CAGCTGCATT

AATGAATCGG

CCAACGCGCG

5701

GGGAGAGGCG

GTTTGCGTAT

TGGGCGCTCT

TCCGCTTCCT

CGCTCACTGA

CTCGCTGCGC

5761

TCGGTCGTTC

GGCTGCGGCG

AGCGGTATCA

GCTCACTCAA

AGGCGGTAAT

ACGGTTATCC

5821

ACAGAATCAG

GGGATAACGC

AGGAAAGAAC

ATGTGAGCAA

AAGGCCAGCA

AAAGGCCAGG

5881

AACCGTAAAA

AGGCCGCGTT

GCTGGCGTTT

TTCCATAGGC

TCCGCCCCCC

TGACGAGCAT

5941

CACAAAAATC

GACGCTCAAG

TCAGAGGTGG

CGAAACCCGA

CAGGACTATA

AAGATACCAG

6001

GCGTTTCCCC

CTGGAAGCTC

CCTCGTGCGC

TCTCCTGTTC

CGACCCTGCC

GCTTACCGGA

6061

TACCTGTCCG

CCTTTCTCCC

TTCGGGAAGC

GTGGCGCTTT

CTCATAGCTC

ACGCTGTAGG

6121

TATCTCAGTT

CGGTGTAGGT

CGTTCGCTCC

AAGCTGGGCT

GTGTGCACGA

ACCCCCCGTT

6181

CAGCCCGACC

GCTGCGCCTT

ATCCGGTAAC

TATCGTCTTG

AGTCCAACCC

GGTAAGACAC

6241

GACTTATCGC

CACTGGCAGC

AGCCACTGGT

AACAGGATTA

GCAGAGCGAG

GTATGTAGGC

6301

GGTGCTACAG

AGTTCTTGAA

GTGGTGGCCT

AACTACGGCT

ACACTAGAAG

GACAGTATTT

6361

GGTATCTGCG

CTCTGCTGAA

GCCAGTTACC

TTCGGAAAAA

GAGTTGGTAG

CTCTTGATCC

6421

GGCAAACAAA

CCACCGCTGG

TAGCGGTGGT

TTTTTTGTTT

GCAAGCAGCA

GATTACGCGC

6481

AGAAAAAAAG

GATCTCAAGA

AGATCCTTTG

ATCTTTTCTA

CGGGGTCTGA

CGCTCAGTGG

6541

AACGAAAACT

CACGTTAAGG

GATTTTGGTC

ATGAGATTAT

CAAAAAGGAT

CTTCACCTAG

6601

ATCCTTTTAA

ATTAAAAATG

AAGTTTTAAA

TCAATCTAAA

GTATATATGA

GTAAACTTGG

6661

TCTGACAGTT

ACCAATGCTT

AATCAGTGAG

GCACCTATCT

CAGCGATCTG

TCTATTTCGT

6721

TCATCCATAG

TTGCCTGACT

CCCCGTCGTG

TAGATAACTA

CGATACGGGA

GGGCTTACCA

6781

TCTGGCCCCA

GTGCTGCAAT

GATACCGCGA

GACCCACGCT

CACCGGCTCC

AGATTTATCA

6841

GCAATAAACC

AGCCAGCCGG

AAGGGCCGAG

CGCAGAAGTG

GTCCTGCAAC

TTTATCCGCC

6901

TCCATCCAGT

CTATTAATTG

TTGCCGGGAA

GCTAGAGTAA

GTAGTTCGCC

AGTTAATAGT

6961

TTGCGCAACG

TTGTTGCCAT

TGCTACAGGC

ATCGTGGTGT

CACGCTCGTC

GTTTGGTATG

7021

GCTTCATTCA

GCTCCGGTTC

CCAACGATCA

AGGCGAGTTA

CATGATCCCC

CATGTTGTGC

7081

AAAAAAGCGG

TTAGCTCCTT

CGGTCCTCCG

ATCGTTGTCA

GAAGTAAGTT

GGCCGCAGTG

7141

TTATCACTCA

TGGTTATGGC

AGCACTGCAT

AATTCTCTTA

CTGTCATGCC

ATCCGTAAGA

7201

TGCTTTTCTG

TGACTGGTGA

GTACTCAACC

AAGTCATTCT

GAGAATAGTG

TATGCGGCGA

7261

CCGAGTTGCT

CTTGCCCGGC

GTCAATACGG

GATAATACCG

CGCCACATAG

CAGAACTTTA

7321

AAAGTGCTCA

TCATTGGAAA

ACGTTCTTCG

GGGCGAAAAC

TCTCAAGGAT

CTTACCGCTG

7381

TTGAGATCCA

GTTCGATGTA

ACCCACTCGT

GCACCCAACT

GATCTTCAGC

ATCTTTTACT

7441

TTCACCAGCG

TTTCTGGGTG

AGCAAAAACA

GGAAGGCAAA

ATGCCGCAAA

AAAGGGAATA

7501

AGGGCGACAC

GGAAATGTTG

AATACTCATA

CTCTTCCTTT

TTCAATATTA

TTGAAGCATT

7561

TATCAGGGTT

ATTGTCTCAT

GAGCGGATAC

ATATTTGAAT

GTATTTAGAA

AAATAAACAA

7621

ATAGGGGTTC

CGCGCACATT

TCCCCGAAAA

GTGCCACCTG

ACGTCTAAGA

AACCATTATT

7681

ATCATGACAT

TAACCTATAA

AAATAGGCGT

ATCACGAGGC

CCTTTCGTCT

CGCGCGTTTC

7741

GGTGATGACG

GTGAAAACCT

CTGACACATG

CAGCTCCCGG

AGACGGTCAC

AGCTTGTCTG

7801

TAAGCGGATG

CCGGGAGCAG

ACAAGCCCGT

CAGGGCGCGT

CAGCGGGTGT

TGGCGGGTGT

7861

CGGGGCTGGC

TTAACTATGC

GGCATCAGAG

CAGATTGTAC

TGAGAGTGCA

CCATATGCGG

7921

TGTGAAATAC

CGCACAGATG

CGTAAGGAGA

AAATACCGCA

TCAGGCGCCA

TTCGCCATTC

7981

AGGCTGCGCA

ACTGTTGGGA

AGGGCGATCG

GTGCGGGCCT

CTTCGCTATT

ACGCCAGCTG

8041

GCGAAAGGGG

GATGTGCTGC

AAGGCGATTA

AGTTGGGTAA

CGCCAGGGTT

TTCCCAGTCA

8101

CGACGTTGTA

AAACGACGGC

CAGTGCCAAG

CTG

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