名称: | pLV3-U6-Atg5(rat)-sgRNA1-Cas9-Hyg |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | Hyg |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLentiCRISPRV2-Hygro |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGCGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GCGCGTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGCTGATC
TTCAGACCTG
GAGGAGGAGA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCGGCACT
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGTTA
ATTAAGGTAC
CGAGGGCCTA
TTTCCCATGA
TTCCTTCATA
TTTGCATATA
CGATACAAGG
CTGTTAGAGA
GATAATTAGA
ATTAATTTGA
CTGTAAACAC
AAAGATATTA
GTACAAAATA
CGTGACGTAG
AAAGTAATAA
TTTCTTGGGT
AGTTTGCAGT
TTTAAAATTA
TGTTTTAAAA
TGGACTATCA
TATGCTTACC
GTAACTTGAA
AGTATTTCGA
TTTCTTGGCT
TTATATATCT
TGTGGAAAGG
ACGAAACACC
GGAAGAGTCA
GCTATTTGAC
GCGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTATCA
ACTTGAAAAA
GTGGCACCGA
GTCGGTGCTT
TTTTGAATTC
GCTAGCTAGG
TCTTGAAAGG
AGTGGGAATT
GGCTCCGGTG
CCCGTCAGTG
GGCAGAGCGC
ACATCGCCCA
CAGTCCCCGA
GAAGTTGGGG
GGAGGGGTCG
GCAATTGATC
CGGTGCCTAG
AGAAGGTGGC
GCGGGGTAAA
CTGGGAAAGT
GATGTCGTGT
ACTGGCTCCG
CCTTTTTCCC
GAGGGTGGGG
GAGAACCGTA
TATAAGTGCA
GTAGTCGCCG
TGAACGTTCT
TTTTCGCAAC
GGGTTTGCCG
CCAGAACACA
GGACCGGTTC
TAGAGCGCTG
CCACCATGGA
CAAGAAGTAC
AGCATCGGCC
TGGACATCGG
CACCAACTCT
GTGGGCTGGG
CCGTGATCAC
CGACGAGTAC
AAGGTGCCCA
GCAAGAAATT
CAAGGTGCTG
GGCAACACCG
ACCGGCACAG
CATCAAGAAG
AACCTGATCG
GAGCCCTGCT
GTTCGACAGC
GGCGAAACAG
CCGAGGCCAC
CCGGCTGAAG
AGAACCGCCA
GAAGAAGATA
CACCAGACGG
AAGAACCGGA
TCTGCTATCT
GCAAGAGATC
TTCAGCAACG
AGATGGCCAA
GGTGGACGAC
AGCTTCTTCC
ACAGACTGGA
AGAGTCCTTC
CTGGTGGAAG
AGGATAAGAA
GCACGAGCGG
CACCCCATCT
TCGGCAACAT
CGTGGACGAG
GTGGCCTACC
ACGAGAAGTA
CCCCACCATC
TACCACCTGA
GAAAGAAACT
GGTGGACAGC
ACCGACAAGG
CCGACCTGCG
GCTGATCTAT
CTGGCCCTGG
CCCACATGAT
CAAGTTCCGG
GGCCACTTCC
TGATCGAGGG
CGACCTGAAC
CCCGACAACA
GCGACGTGGA
CAAGCTGTTC
ATCCAGCTGG
TGCAGACCTA
CAACCAGCTG
TTCGAGGAAA
ACCCCATCAA
CGCCAGCGGC
GTGGACGCCA
AGGCCATCCT
GTCTGCCAGA
CTGAGCAAGA
GCAGACGGCT
GGAAAATCTG
ATCGCCCAGC
TGCCCGGCGA
GAAGAAGAAT
GGCCTGTTCG
GAAACCTGAT
TGCCCTGAGC
CTGGGCCTGA
CCCCCAACTT
CAAGAGCAAC
TTCGACCTGG
CCGAGGATGC
CAAACTGCAG
CTGAGCAAGG
ACACCTACGA
CGACGACCTG
GACAACCTGC
TGGCCCAGAT
CGGCGACCAG
TACGCCGACC
TGTTTCTGGC
CGCCAAGAAC
CTGTCCGACG
CCATCCTGCT
GAGCGACATC
CTGAGAGTGA
ACACCGAGAT
CACCAAGGCC
CCCCTGAGCG
CCTCTATGAT
CAAGAGATAC
GACGAGCACC
ACCAGGACCT
GACCCTGCTG
AAAGCTCTCG
TGCGGCAGCA
GCTGCCTGAG
AAGTACAAAG
AGATTTTCTT
CGACCAGAGC
AAGAACGGCT
ACGCCGGCTA
CATTGACGGC
GGAGCCAGCC
AGGAAGAGTT
CTACAAGTTC
ATCAAGCCCA
TCCTGGAAAA
GATGGACGGC
ACCGAGGAAC
TGCTCGTGAA
GCTGAACAGA
GAGGACCTGC
TGCGGAAGCA
GCGGACCTTC
GACAACGGCA
GCATCCCCCA
CCAGATCCAC
CTGGGAGAGC
TGCACGCCAT
TCTGCGGCGG
CAGGAAGATT
TTTACCCATT
CCTGAAGGAC
AACCGGGAAA
AGATCGAGAA
GATCCTGACC
TTCCGCATCC
CCTACTACGT
GGGCCCTCTG
GCCAGGGGAA
ACAGCAGATT
CGCCTGGATG
ACCAGAAAGA
GCGAGGAAAC
CATCACCCCC
TGGAACTTCG
AGGAAGTGGT
GGACAAGGGC
GCTTCCGCCC
AGAGCTTCAT
CGAGCGGATG
ACCAACTTCG
ATAAGAACCT
GCCCAACGAG
AAGGTGCTGC
CCAAGCACAG
CCTGCTGTAC
GAGTACTTCA
CCGTGTATAA
CGAGCTGACC
AAAGTGAAAT
ACGTGACCGA
GGGAATGAGA
AAGCCCGCCT
TCCTGAGCGG
CGAGCAGAAA
AAGGCCATCG
TGGACCTGCT
GTTCAAGACC
AACCGGAAAG
TGACCGTGAA
GCAGCTGAAA
GAGGACTACT
TCAAGAAAAT
CGAGTGCTTC
GACTCCGTGG
AAATCTCCGG
CGTGGAAGAT
CGGTTCAACG
CCTCCCTGGG
CACATACCAC
GATCTGCTGA
AAATTATCAA
GGACAAGGAC
TTCCTGGACA
ATGAGGAAAA
CGAGGACATT
CTGGAAGATA
TCGTGCTGAC
CCTGACACTG
TTTGAGGACA
GAGAGATGAT
CGAGGAACGG
CTGAAAACCT
ATGCCCACCT
GTTCGACGAC
AAAGTGATGA
AGCAGCTGAA
GCGGCGGAGA
TACACCGGCT
GGGGCAGGCT
GAGCCGGAAG
CTGATCAACG
GCATCCGGGA
CAAGCAGTCC
GGCAAGACAA
TCCTGGATTT
CCTGAAGTCC
GACGGCTTCG
CCAACAGAAA
CTTCATGCAG
CTGATCCACG
ACGACAGCCT
GACCTTTAAA
GAGGACATCC
AGAAAGCCCA
GGTGTCCGGC
CAGGGCGATA
GCCTGCACGA
GCACATTGCC
AATCTGGCCG
GCAGCCCCGC
CATTAAGAAG
GGCATCCTGC
AGACAGTGAA
GGTGGTGGAC
GAGCTCGTGA
AAGTGATGGG
CCGGCACAAG
CCCGAGAACA
TCGTGATCGA
AATGGCCAGA
GAGAACCAGA
CCACCCAGAA
GGGACAGAAG
AACAGCCGCG
AGAGAATGAA
GCGGATCGAA
GAGGGCATCA
AAGAGCTGGG
CAGCCAGATC
CTGAAAGAAC
ACCCCGTGGA
AAACACCCAG
CTGCAGAACG
AGAAGCTGTA
CCTGTACTAC
CTGCAGAATG
GGCGGGATAT
GTACGTGGAC
CAGGAACTGG
ACATCAACCG
GCTGTCCGAC
TACGATGTGG
ACCATATCGT
GCCTCAGAGC
TTTCTGAAGG
ACGACTCCAT
CGACAACAAG
GTGCTGACCA
GAAGCGACAA
GAACCGGGGC
AAGAGCGACA
ACGTGCCCTC
CGAAGAGGTC
GTGAAGAAGA
TGAAGAACTA
CTGGCGGCAG
CTGCTGAACG
CCAAGCTGAT
TACCCAGAGA
AAGTTCGACA
ATCTGACCAA
GGCCGAGAGA
GGCGGCCTGA
GCGAACTGGA
TAAGGCCGGC
TTCATCAAGA
GACAGCTGGT
GGAAACCCGG
CAGATCACAA
AGCACGTGGC
ACAGATCCTG
GACTCCCGGA
TGAACACTAA
GTACGACGAG
AATGACAAGC
TGATCCGGGA
AGTGAAAGTG
ATCACCCTGA
AGTCCAAGCT
GGTGTCCGAT
TTCCGGAAGG
ATTTCCAGTT
TTACAAAGTG
CGCGAGATCA
ACAACTACCA
CCACGCCCAC
GACGCCTACC
TGAACGCCGT
CGTGGGAACC
GCCCTGATCA
AAAAGTACCC
TAAGCTGGAA
AGCGAGTTCG
TGTACGGCGA
CTACAAGGTG
TACGACGTGC
GGAAGATGAT
CGCCAAGAGC
GAGCAGGAAA
TCGGCAAGGC
TACCGCCAAG
TACTTCTTCT
ACAGCAACAT
CATGAACTTT
TTCAAGACCG
AGATTACCCT
GGCCAACGGC
GAGATCCGGA
AGCGGCCTCT
GATCGAGACA
AACGGCGAAA
CCGGGGAGAT
CGTGTGGGAT
AAGGGCCGGG
ATTTTGCCAC
CGTGCGGAAA
GTGCTGAGCA
TGCCCCAAGT
GAATATCGTG
AAAAAGACCG
AGGTGCAGAC
AGGCGGCTTC
AGCAAAGAGT
CTATCCTGCC
CAAGAGGAAC
AGCGATAAGC
TGATCGCCAG
AAAGAAGGAC
TGGGACCCTA
AGAAGTACGG
CGGCTTCGAC
AGCCCCACCG
TGGCCTATTC
TGTGCTGGTG
GTGGCCAAAG
TGGAAAAGGG
CAAGTCCAAG
AAACTGAAGA
GTGTGAAAGA
GCTGCTGGGG
ATCACCATCA
TGGAAAGAAG
CAGCTTCGAG
AAGAATCCCA
TCGACTTTCT
GGAAGCCAAG
GGCTACAAAG
AAGTGAAAAA
GGACCTGATC
ATCAAGCTGC
CTAAGTACTC
CCTGTTCGAG
CTGGAAAACG
GCCGGAAGAG
AATGCTGGCC
TCTGCCGGCG
AACTGCAGAA
GGGAAACGAA
CTGGCCCTGC
CCTCCAAATA
TGTGAACTTC
CTGTACCTGG
CCAGCCACTA
TGAGAAGCTG
AAGGGCTCCC
CCGAGGATAA
TGAGCAGAAA
CAGCTGTTTG
TGGAACAGCA
CAAGCACTAC
CTGGACGAGA
TCATCGAGCA
GATCAGCGAG
TTCTCCAAGA
GAGTGATCCT
GGCCGACGCT
AATCTGGACA
AAGTGCTGTC
CGCCTACAAC
AAGCACCGGG
ATAAGCCCAT
CAGAGAGCAG
GCCGAGAATA
TCATCCACCT
GTTTACCCTG
ACCAATCTGG
GAGCCCCTGC
CGCCTTCAAG
TACTTTGACA
CCACCATCGA
CCGGAAGAGG
TACACCAGCA
CCAAAGAGGT
GCTGGACGCC
ACCCTGATCC
ACCAGAGCAT
CACCGGCCTG
TACGAGACAC
GGATCGACCT
GTCTCAGCTG
GGAGGCGACA
AGCGACCTGC
CGCCACAAAG
AAGGCTGGAC
AGGCTAAGAA
GAAGAAAGAT
TACAAAGACG
ATGACGATAA
GGGATCCGGC
GCAACAAACT
TCTCTCTGCT
GAAACAAGCC
GGAGATGTCG
AAGAGAATCC
TGGACCGAAA
AAGCCTGAAC
TCACCGCGAC
GTCTGTCGAG
AAGTTTCTGA
TCGAAAAGTT
CGACAGCGTG
TCCGACCTGA
TGCAGCTCTC
GGAGGGCGAA
GAATCTCGTG
CTTTCAGCTT
CGATGTAGGA
GGGCGTGGAT
ATGTCCTGCG
GGTAAATAGC
TGCGCCGATG
GTTTCTACAA
AGATCGTTAT
GTTTATCGGC
ACTTTGCATC
GGCCGCGCTC
CCGATTCCGG
AAGTGCTTGA
CATTGGGGAA
TTCAGCGAGA
GCCTGACCTA
TTGCATCTCC
CGCCGTGCAC
AGGGTGTCAC
GTTGCAAGAC
CTGCCTGAAA
CCGAACTGCC
CGCTGTTCTG
CAGCCGGTCG
CGGAGGCCAT
GGATGCGATC
GCTGCGGCCG
ATCTTAGCCA
GACGAGCGGG
TTCGGCCCAT
TCGGACCGCA
AGGAATCGGT
CAATACACTA
CATGGCGTGA
TTTCATATGC
GCGATTGCTG
ATCCCCATGT
GTATCACTGG
CAAACTGTGA
TGGACGACAC
CGTCAGTGCG
TCCGTCGCGC
AGGCTCTCGA
TGAGCTGATG
CTTTGGGCCG
AGGACTGCCC
CGAAGTCCGG
CACCTCGTGC
ACGCGGATTT
CGGCTCCAAC
AATGTCCTGA
CGGACAATGG
CCGCATAACA
GCGGTCATTG
ACTGGAGCGA
GGCGATGTTC
GGGGATTCCC
AATACGAGGT
CGCCAACATC
TTCTTCTGGA
GGCCGTGGTT
GGCTTGTATG
GAGCAGCAGA
CGCGCTACTT
CGAGCGGAGG
CATCCGGAGC
TTGCAGGATC
GCCGCGGCTC
CGGGCGTATA
TGCTCCGCAT
TGGTCTTGAC
CAACTCTATC
AGAGCTTGGT
TGACGGCAAT
TTCGATGATG
CAGCTTGGGC
GCAGGGTCGA
TGCGACGCAA
TCGTCCGATC
CGGAGCCGGG
ACTGTCGGGC
GTACACAAAT
CGCCCGCAGA
AGCGCGGCCG
TCTGGACCGA
TGGCTGTGTA
GAAGTACTTC
GCCGATAGAC
GCGTTAAGTC
GACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCGTCGACTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGGG
CCCGTTTAAA
CCCGCTGATC
AGCCTCGACT
GTGCCTTCTA
GTTGCCAGCC
ATCTGTTGTT
TGCCCCTCCC
CCGTGCCTTC
CTTGACCCTG
GAAGGTGCCA
CTCCCACTGT
CCTTTCCTAA
TAAAATGAGG
AAATTGCATC
GCATTGTCTG
AGTAGGTGTC
ATTCTATTCT
GGGGGGTGGG
GTGGGGCAGG
ACAGCAAGGG
GGAGGATTGG
GAAGACAATA
GCAGGCATGC
TGGGGATGCG
GTGGGCTCTA
TGGCTTCTGA
GGCGGAAAGA
ACCAGCTGGG
GCTCTAGGGG
GTATCCCCAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTAAT
TCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCT
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
AAGCTCCCGG
GAGCTTGTAT
ATCCATTTTC
GGATCTGATC
AGCACGTGTT
GACAATTAAT
CATCGGCATA
GTATATCGGC
ATAGTATAAT
ACGACAAGGT
GAGGAACTAA
ACCATGGCCA
AGTTGACCAG
TGCCGTTCCG
GTGCTCACCG
CGCGCGACGT
CGCCGGAGCG
GTCGAGTTCT
GGACCGACCG
GCTCGGGTTC
TCCCGGGACT
TCGTGGAGGA
CGACTTCGCC
GGTGTGGTCC
GGGACGACGT
GACCCTGTTC
ATCAGCGCGG
TCCAGGACCA
GGTGGTGCCG
GACAACACCC
TGGCCTGGGT
GTGGGTGCGC
GGCCTGGACG
AGCTGTACGC
CGAGTGGTCG
GAGGTCGTGT
CCACGAACTT
CCGGGACGCC
TCCGGGCCGG
CCATGACCGA
GATCGGCGAG
CAGCCGTGGG
GGCGGGAGTT
CGCCCTGCGC
GACCCGGCCG
GCAACTGCGT
GCACTTCGTG
GCCGAGGAGC
AGGACTGACA
CGTGCTACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGTA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
AC