名称: | pLV3-CMV-SUPT16H(human-Toxic-opt)-3×FLAG-EF1a-mCherry-Blast |
---|---|
别称: | NM_007192.4;CDC68; FACTP140; NEDDFAC; SPT16;SPT16/CDC68 |
基因长: | 3141bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | 红色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-EF1a-mCherry-Blast |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
GAATTCGCCA
CCATGGCTGT
GACTCTGGAC
AAAGACGCTT
ATTATCGGCG
AGTGAAGAGA
CTGTACAGCA
ATTGGCGGAA
AGGAGAAGAT
GAGTATGCCA
ACGTTGATGC
CATTGTTGTA
TCAGTGGGTG
TTGATGAAGA
AATTGTTTAT
GCCAAATCAA
CTGCCTTACA
GACATGGCTC
TTTGGTTATG
AACTAACTGA
TACTATCATG
GTCTTTTGTG
ATGACAAAAT
CATCTTTATG
GCCAGCAAGA
AAAAAGTGGA
GTTCTTGAAA
CAGATTGCCA
ACACTAAGGG
CAATGAGAAT
GCTAATGGAG
CCCCTGCCAT
CACACTGCTA
ATACGAGAAA
AGAATGAAAG
TAATAAGAGT
AGCTTTGACA
AAATGATTGA
AGCCATTAAA
GAAAGCAAGA
ATGGCAAGAA
GATTGGAGTG
TTCAGCAAAG
ACAAATTCCC
TGGAGAGTTC
ATGAAGAGCT
GGAATGACTG
CCTCAACAAA
GAAGGCTTTG
ACAAAATAGA
TATCAGTGCA
GTTGTGGCAT
ATACCATCGC
TGTAAAGGAG
GATGGGGAGC
TCAACCTAAT
GAAGAAAGCA
GCCAGCATCA
CTTCTGAAGT
CTTCAACAAA
TTCTTCAAGG
AAAGAGTCAT
GGAAATAGTT
GATGCAGATG
AGAAAGTTCG
ACACAGCAAA
CTGGCTGAGT
CTGTGGAAAA
GGCCATTGAA
GAGAAAAAAT
ACCTTGCTGG
GGCAGACCCT
TCTACTGTGG
AAATGTGTTA
CCCTCCTATC
ATTCAGAGTG
GTGGCAACTA
TAATCTCAAG
TTCAGTGTGG
TGAGTGACAA
GAATCATATG
CACTTTGGGG
CTATCACTTG
TGCCATGGGT
ATTCGCTTCA
AGTCTTACTG
CTCCAACCTT
GTTCGCACTT
TGATGGTTGA
TCCTTCTCAA
GAAGTTCAAG
AAAATTATAA
CTTTTTGCTC
CAGCTTCAAG
AGGAGCTGCT
GAAGGAATTA
AGACATGGTG
TGAAGATATG
TGACGTGTAT
AACGCTGTCA
TGGACGTGGT
TAAAAAGCAG
AAGCCAGAAC
TGCTGAACAA
AATTACCAAA
AACCTAGGGT
TTGGGATGGG
AATTGAATTC
CGTGAAGGCT
CCCTAGTAAT
CAATAGCAAA
AATCAATACA
AACTGAAGAA
AGGAATGGTT
TTCAGCATCA
ATTTAGGATT
CTCAGACCTG
ACTAACAAGG
AGGGGAAAAA
GCCAGAAGAG
AAAACCTATG
CCCTGTTCAT
TGGTGACACA
GTGCTTGTGG
ATGAGGATGG
CCCAGCTACT
GTTCTCACTT
CTGTGAAGAA
GAAAGTGAAG
AATGTGGGGA
TTTTCCTAAA
GAATGAAGAT
GAGGAAGAAG
AGGAGGAGGA
GAAAGATGAG
GCAGAGGACC
TTTTGGGAAG
AGGTTCTCGG
GCAGCATTAC
TTACAGAAAG
AACAAGAAAT
GAAATGACTG
CAGAAGAGAA
GCGAAGAGCA
CATCAGAAAG
AACTAGCGGC
TCAACTCAAT
GAAGAAGCAA
AGAGGCGATT
GACTGAACAA
AAGGGAGAAC
AGCAGATTCA
GAAAGCTCGC
AAGTCTAATG
TGTCCTATAA
AAACCCATCT
CTGATGCCTA
AGGAACCACA
TATTCGGGAA
ATGAAGATCT
ACATCGATAA
GAAATATGAG
ACTGTAATAA
TGCCCGTGTT
TGGCATTGCA
ACACCGTTTC
ACATTGCCAC
AATCAAGAAT
ATAAGTATGT
CAGTTGAAGG
CGATTATACA
TATCTGCGAA
TTAACTTTTA
CTGCCCAGGA
TCCGCCTTGG
GGAGGAATGA
GGGAAACATC
TTCCCTAATC
CAGAGGCCAC
GTTTGTCAAA
GAAATTACCT
ACAGGGCATC
CAATATTAAG
GCTCCAGGAG
AACAGACTGT
GCCTGCTCTG
AATCTGCAGA
ATGCATTCCG
CATAATCAAA
GAAGTGCAGA
AGAGGTATAA
GACTAGGGAG
GCTGAGGAGA
AAGAAAAAGA
GGGTATTGTG
AAGCAGGATT
CCCTCGTGAT
CAACCTGAAC
AGGAGCAATC
CTAAACTGAA
AGACCTGTAT
ATACGCCCCA
ACATTGCTCA
AAAACGCATG
CAGGGGAGCC
TTGAAGCCCA
TGTGAACGGG
TTTAGGTTCA
CGTCCGTGAG
AGGCGATAAG
GTCGATATCC
TGTACAACAA
CATCAAACAC
GCTTTGTTTC
AGCCTTGTGA
CGGGGAGATG
ATCATCGTCC
TGCATTTCCA
TCTGAAGAAC
GCTATCATGT
TCGGCAAGAA
GCGCCACACA
GACGTGCAGT
TCTATACCGA
GGTGGGAGAG
ATCACAACCG
ATCTGGGAAA
GCACCAGCAC
ATGCATGACC
GCGATGATCT
GTATGCCGAG
CAGATGGAGA
GAGAGATGAG
ACACAAGCTG
AAGACAGCCT
TCAAGAACTT
CATCGAGAAA
GTGGAGGCCC
TGACCAAAGA
AGAGCTGGAG
TTCGAGGTGC
CCTTCAGAGA
TCTGGGCTTT
AATGGGGCCC
CCTACAGGTC
AACCTGCCTG
CTGCAGCCAA
CCAGCTCAGC
CCTGGTGAAT
GCCACAGAAT
GGCCACCCTT
TGTGGTGACC
CTGGACGAGG
TGGAACTGAT
TCACTTTGAG
AGGGTGCAGT
TCCACCTGAA
GAACTTTGAC
ATGGTGATCG
TGTACAAGGA
CTACTCTAAA
AAGGTGACAA
TGATAAATGC
CATCCCAGTT
GCCTCCCTCG
ATCCCATAAA
AGAGTGGCTC
AACAGTTGCG
ATCTCAAATA
TACCGAAGGC
GTGCAATCCC
TCAATTGGAC
CAAGATCATG
AAGACGATCG
TAGATGACCC
AGAAGGTTTC
TTCGAGCAAG
GCGGTTGGTC
CTTTCTGGAG
CCGGAGGGTG
AAGGGTCTGA
TGCCGAAGAA
GGCGACAGCG
AATCTGAGAT
TGAGGATGAG
ACGTTCAATC
CGAGTGAGGA
TGATTACGAG
GAAGAGGAAG
AGGACAGTGA
TGAGGACTAT
TCCTCAGAAG
CGGAGGAAAG
TGACTATTCC
AAGGAAAGTC
TGGGCAGCGA
AGAGGAGAGC
GGCAAAGATT
GGGACGAACT
TGAAGAGGAA
GCTAGGAAAG
CCGATAGGGA
ATCAAGGTAT
GAGGAAGAGG
AAGAGCAAAG
CAGATCAATG
TCTCGCAAAA
GGAAGGCAAG
CGTGCATTCT
TCTGGTAGAG
GTTCTAACAG
GGGCAGCCGG
CATTCATCAG
CTCCTCCTAA
GAAGAAAAGG
AAAGGATCCG
ACTACAAAGA
CCATGACGGT
GATTATAAAG
ATCATGACAT
CGACTACAAG
GATGACGATG
ACAAGTAGCG
GCCGCGAAGG
ATCTGCGATC
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACG
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
CTGAAGCTTC
GAGGGGCTCG
CATCTCTCCT
TCACGCGCCC
GCCGCCCTAC
CTGAGGCCGC
CATCCACGCC
GGTTGAGTCG
CGTTCTGCCG
CCTCCCGCCT
GTGGTGCCTC
CTGAACTGCG
TCCGCCGTCT
AGGTAAGTTT
AAAGCTCAGG
TCGAGACCGG
GCCTTTGTCC
GGCGCTCCCT
TGGAGCCTAC
CTAGACTCAG
CCGGCTCTCC
ACGCTTTGCC
TGACCCTGCT
TGCTCAACTC
TACGTCTTTG
TTTCGTTTTC
TGTTCTGCGC
CGTTACAGAT
CCAAGCTGTG
ACCGGCGCCT
ACGCTAGACG
CCACCATGGT
GAGCAAGGGC
GAGGAGGATA
ACATGGCCAT
CATCAAGGAG
TTCATGCGCT
TCAAGGTGCA
CATGGAGGGC
TCCGTGAACG
GCCACGAGTT
CGAGATCGAG
GGCGAGGGCG
AGGGCCGCCC
CTACGAGGGC
ACCCAGACCG
CCAAGCTGAA
GGTGACCAAG
GGTGGCCCCC
TGCCCTTCGC
CTGGGACATC
CTGTCCCCTC
AGTTCATGTA
CGGCTCCAAG
GCCTACGTGA
AGCACCCCGC
CGACATCCCC
GACTACTTGA
AGCTGTCCTT
CCCCGAGGGC
TTCAAGTGGG
AGCGCGTGAT
GAACTTCGAG
GACGGCGGCG
TGGTGACCGT
GACCCAGGAC
TCCTCCCTGC
AGGACGGCGA
GTTCATCTAC
AAGGTGAAGC
TGCGCGGCAC
CAACTTCCCC
TCCGACGGCC
CCGTAATGCA
GAAGAAGACC
ATGGGCTGGG
AGGCCTCCTC
CGAGCGGATG
TACCCCGAGG
ACGGCGCCCT
GAAGGGCGAG
ATCAAGCAGA
GGCTGAAGCT
GAAGGACGGC
GGCCACTACG
ACGCTGAGGT
CAAGACCACC
TACAAGGCCA
AGAAGCCCGT
GCAGCTGCCC
GGCGCCTACA
ACGTCAACAT
CAAGTTGGAC
ATCACCTCCC
ACAACGAGGA
CTACACCATC
GTGGAACAGT
ACGAACGCGC
CGAGGGCCGC
CACTCCACCG
GCGGCATGGA
CGAGCTGTAC
AAGGGATCTC
GCGAGGGCAG
AGGAAGTCTT
CTAACATGCG
GTGACGTGGA
GGAGAATCCC
GGCCCTATGG
CCAAGCCTTT
GTCTCAAGAA
GAATCCACCC
TCATTGAAAG
AGCAACGGCT
ACAATCAACA
GCATCCCCAT
CTCTGAAGAC
TACAGCGTCG
CCAGCGCAGC
TCTCTCTAGC
GACGGCCGCA
TCTTCACTGG
TGTCAATGTA
TATCATTTTA
CTGGGGGACC
TTGTGCAGAA
CTCGTGGTGC
TGGGCACTGC
TGCTGCTGCG
GCAGCTGGCA
ACCTGACTTG
TATCGTCGCG
ATCGGAAATG
AGAACAGGGG
CATCTTGAGC
CCCTGCGGAC
GGTGCCGACA
GGTGCTTCTC
GATCTGCATC
CTGGGATCAA
AGCCATAGTG
AAGGACAGTG
ATGGACAGCC
GACGGCAGTT
GGGATTCGTG
AATTGCTGCC
CTCTGGTTAT
GTGTGGGAGG
GCTAAGTCGA
CAATCAACCT
CTGGATTACA
AAATTTGTGA
AAGATTGACT
GGTATTCTTA
ACTATGTTGC
TCCTTTTACG
CTATGTGGAT
ACGCTGCTTT
AATGCCTTTG
TATCATGCTA
TTGCTTCCCG
TATGGCTTTC
ATTTTCTCCT
CCTTGTATAA
ATCCTGGTTG
CTGTCTCTTT
ATGAGGAGTT
GTGGCCCGTT
GTCAGGCAAC
GTGGCGTGGT
GTGCACTGTG
TTTGCTGACG
CAACCCCCAC
TGGTTGGGGC
ATTGCCACCA
CCTGTCAGCT
CCTTTCCGGG
ACTTTCGCTT
TCCCCCTCCC
TATTGCCACG
GCGGAACTCA
TCGCCGCCTG
CCTTGCCCGC
TGCTGGACAG
GGGCTCGGCT
GTTGGGCACT
GACAATTCCG
TGGTGTTGTC
GGGGAAGCTG
ACGTCCTTTC
CATGGCTGCT
CGCCTGTGTT
GCCACCTGGA
TTCTGCGCGG
GACGTCCTTC
TGCTACGTCC
CTTCGGCCCT
CAATCCAGCG
GACCTTCCTT
CCCGCGGCCT
GCTGCCGGCT
CTGCGGCCTC
TTCCGCGTCT
CCGCCTTCGC
CCTCAGACGA
GTCGGATCTC
CCTTTGGCCG
CCTCCCCGCC
TGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ATAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGC
TCTAGCTATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCGGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGACTT
TTGCAGAGAC
GGCCCAAATT
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGGACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCTTTCG
TCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAGACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATATG
CGGTGTGAAA
TACCGCACAG
ATGCGTAAGG
AGAAAATACC
GCATCAGGCG
CCATTCGCCA
TTCAGGCTGC
GCAACTGTTG
GGAAGGGCGA
TCGGTGCGGG
CCTCTTCGCT
ATTACGCCAG
CTGGCGAAAG
GGGGATGTGC
TGCAAGGCGA
TTAAGTTGGG
TAACGCCAGG
GTTTTCCCAG
TCACGACGTT
GTAAAACGAC
GGCCAGTGCC
AAGCTG