名称: | pCMV-DOCK10(human)-3×FLAG-Neo |
---|---|
别称: | NM_014689.3 NP_055504.2 55619 DOCK10 DOCK10/DRIP2/Nbla10300/ZIZ3 |
基因长: | 6561bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | 低 |
感受态: | BL21(DE3) |
温度: | 30度 |
背景载体: | pCMV-T7-MCS-3×FLAG-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGCCACCAT
GGCCGGTGAG
CGGACCCGCA
GGTTCACCCG
GAGCCTGTTG
AGACCTGGGC
AGGCGGCCGA
GCTCCGGCAC
AGCGCCGCGT
CCGCCGCCGC
GGTGGCAGTC
AGCAGCCGGC
AGCAGCAGCG
GCAAGAAAAG
CCTAGGCTTC
TCGAGCCTTT
GGATTATGAG
ACTGTCATTG
AAGAACTTGA
AAAGACCTAC
CGGAATGATC
CTCTTCAAGA
TCTCTTGTTC
TTCCCCAGTG
ATGACTTTTC
AGCAGCCACA
GTTTCCTGGG
ATATCCGCAC
GTTGTACTCA
ACAGTACCTG
AAGATGCAGA
GCACAAGGCA
GAAAATTTAC
TGGTTAAGGA
GGCTTGTAAA
TTTTATAGTT
CCCAGTGGCA
TGTGGTAAAC
TACAAATATG
AACAATATTC
TGGAGACATT
CGACAGCTAC
CCCGAGCAGA
ATACAAACCA
GAGAAGCTTC
CTTCACATTC
CTTTGAGATT
GACCATGAAG
ATGCTGATAA
GGATGAAGAT
ACCACTTCCC
ACTCGTCTTC
CAAGGGGGGT
GGAGGAGCGG
GAGGAACTGG
TGTTTTCAAG
TCCGGCTGGC
TCTACAAGGG
GAATTTTAAC
AGCACCGTGA
ACAACACCGT
TACTGTTCGG
TCATTCAAAA
AGCGCTACTT
CCAGCTGACT
CAGTTACCAG
ATAACTCCTA
CATTATGAAC
TTTTACAAAG
ATGAGAAAAT
ATCCAAAGAA
CCCAAAGGAT
GCATCTTTTT
GGATTCCTGT
ACAGGAGTGG
TGCAGAATAA
CAGACTAAGA
AAATATGCCT
TTGAATTGAA
AATGAATGAT
CTGACCTATT
TTGTGCTGGC
AGCTGAAACA
GAGTCAGATA
TGGATGAATG
GATCCACACC
CTCAACCGCA
TTCTGCAAAT
CAGTCCTGAG
GGGCCCCTCC
AAGGGAGGAG
GAGCACAGAG
CTCACTGATC
TGGGTCTGGA
TTCGCTGGAT
AATTCTGTAA
CTTGTGAATG
CACGCCAGAG
GAAACAGATT
CTTCAGAGAA
CAACCTACAC
GCAGACTTTG
CAAAGTACCT
CACAGAAACA
GAAGATACTG
TAAAAACAAC
TCGAAACATG
GAGAGGCTAA
ATCTGTTCTC
TCTAGATCCA
GACATAGATA
CCTTGAAACT
TCAAAAAAAA
GATCTCTTGG
AACCTGAGTC
TGTGATCAAA
CCATTTGAAG
AAAAAGCTGC
CAAGAGAATC
ATGATCATCT
GTAAAGCCCT
CAACTCAAAT
CTTCAGGGAT
GTGTTACGGA
GAATGAAAAT
GATCCGATAA
CGAATATTGA
GCCTTTTTTT
GTGAGTGTGG
CACTTTATGA
CCTCAGAGAC
AGCAGGAAGA
TTTCTGCTGA
TTTTCATGTG
GATCTAAACC
ATGCTGCTGT
CAGACAGATG
CTCTTGGGGG
CTTCTGTGGC
TTTGGAAAAT
GGCAACATCG
ACACCATCAC
TCCAAGACAA
TCAGAAGAAC
CTCACATCAA
GGGACTTCCA
GAGGAATGGC
TAAAATTTCC
AAAGCAGGCT
GTATTTTCTG
TAAGCAATCC
ACATTCTGAA
ATTGTTTTGG
TGGCCAAAAT
CGAAAAAGTC
TTGATGGGAA
ACATTGCAAG
TGGTGCCGAA
CCTTATATTA
AGAACCCAGA
CTCCAACAAG
TATGCACAAA
AGATACTAAA
ATCCAACAGA
CAATTCTGCA
GCAAATTGGG
AAAATACCGT
ATGCCTTTTG
CTTGGGCAGT
AAGATCAGTA
TTTAAGGACA
ACCAGGGAAA
TGTGGACAGA
GACTCAAGAT
TTTCACCATT
GTTTAGACAA
GAAAGTAGCA
AGATTTCAAC
TGAGGACCTA
GTTAAACTAG
TATCAGATTA
TAGAAGGGCC
GACAGAATAA
GCAAAATGCA
GACCATTCCT
GGAAGCCTGG
ATATTGCTGT
TGACAACGTT
CCCTTGGAGC
ATCCAAATTG
TGTAACATCG
TCCTTTATCC
CTGTCAAGCC
TTTCAACATG
ATGGCTCAAA
CAGAACCCAC
AGTGGAGGTG
GAAGAATTTG
TTTACGATTC
AACAAAGTAT
TGTCGGCCTT
ACAGAGTATA
TAAAAATCAA
ATTTATATTT
ACCCCAAACA
CCTCAAGTAT
GATAGCCAGA
AATGCTTCAA
CAAGGCACGG
AATATAACTG
TGTGCATTGA
ATTCAAAAAT
TCAGATGAAG
AAAGTGCCAA
GCCCCTGAAG
TGTATTTATG
GAAAACCTGG
AGGGCCCCTC
TTCACCTCAG
CCGCCTACAC
AGCAGTTCTG
CACCACTCTC
AGAATCCGGA
TTTCTCAGAT
GAGGTGAAAA
TTGAGCTACC
AACACAACTC
CATGAGAAAC
ACCATATTTT
GTTTTCTTTT
TATCACGTCA
CCTGTGACAT
CAATGCAAAA
GCTAATGCCA
AAAAGAAGGA
GGCTCTGGAA
ACGTCAGTTG
GATATGCTTG
GCTTCCTCTG
ATGAAACACG
ATCAGATAGC
TTCTCAAGAG
TACAACATCC
CAATAGCAAC
AAGTCTGCCT
CCTAATTATT
TAAGCTTTCA
AGATTCTGCA
AGTGGAAAGC
ATGGTGGGAG
TGACATTAAA
TGGGTTGATG
GTGGCAAACC
ACTTTTCAAA
GTGTCGACAT
TTGTTGTATC
AACAGTAAAT
ACTCAGGATC
CACATGTGAA
TGCATTTTTC
CAAGAGTGCC
AAAAAAGAGA
GAAAGATATG
TCTCAGTCAC
CTACCTCAAA
TTTCATCCGC
TCTTGTAAGA
ACTTATTGAA
TGTGGAAAAG
ATTCATGCAA
TCATGAGTTT
TCTGCCTATA
ATTTTGAATC
AGCTCTTCAA
AGTTCTGGTA
CAGAATGAGG
AAGATGAAAT
AACTACAACT
GTCACCAGGG
TTCTGACCGA
CATTGTGGCC
AAGTGCCATG
AGGAGCAGCT
GGATCATTCT
GTCCAGTCAT
ATATTAAGTT
CGTGTTCAAG
ACCAGGGCAT
GCAAGGAGAG
GACTGTACAT
GAGGAACTGG
CTAAAAATGT
GACTGGTCTT
TTGAAATCAA
ATGACTCAAC
AACAGTAAAG
CATGTCCTAA
AGCATTCCTG
GTTCTTCTTT
GCAATTATCC
TAAAATCGAT
GGCACAGCAC
TTGATTGACA
CAAATAAAAT
CCAGCTTCCC
CGGCCTCAGA
GATTTCCTGA
ATCTTACCAA
AATGAATTGG
ACAATCTTGT
CATGGTCCTA
TCCGACCATG
TGATTTGGAA
ATACAAGGAT
GCACTTGAAG
AAACAAGAAG
GGCAAACCAC
AGCGTTGCCA
GATTTCTCAA
GCGCTGCTTT
ACATTTATGG
ACCGAGGGTA
TGTGTTTAAG
ATGGTCAACA
ATTACATCAG
CATGTTCTCC
TCCGGTGACC
TTAAGACCTT
GTGCCAGTAT
AAATTTGATT
TTCTTCAAGA
AGTATGTCAA
CATGAACACT
TTATCCCTTT
GTGTCTGCCC
ATAAGATCAG
CAAACATTCC
AGATCCTTTG
ACACCTTCAG
AATCGACTCA
AGAGTTACAT
GCATCAGATA
TGCCTGAATA
TTCAGTCACA
AATGAATTTT
GTCGCAAACA
CTTCTTAATC
GGAATTCTGC
TCCGAGAAGT
TGGCTTTGCC
CTGCAGGAAG
ACCAAGATGT
CAGACACTTA
GCTTTAGCTG
TCCTAAAAAA
TCTAATGGCT
AAGCATTCAT
TTGATGATCG
ATACAGAGAG
CCAAGAAAGC
AGGCCCAGAT
AGCAAGTTTA
TACATGCCCC
TGTACGGCAT
GCTCCTGGAC
AATATGCCAA
GGATTTATCT
GAAGGACCTG
TATCCTTTTA
CTGTCAATAC
ATCTAATCAG
GGGTCTAGAG
ATGATCTAAG
CACCAATGGA
GGATTTCAAA
GCCAGACAGC
TATCAAACAT
GCAAACTCTG
TGGATACATC
ATTTTCTAAA
GATGTTTTAA
ATTCCATAGC
AGCATTTTCA
TCAATAGCTA
TTTCTACAGT
AAACCATGCT
GACTCCAGAG
CATCTTTAGC
AAGTCTTGAC
TCCAATCCAA
GTACCAATGA
GAAGAGCAGT
GAGAAGACGG
ACAACTGTGA
AAAGATCCCA
AGACCCTTGT
CTTTGATTGG
CTCAACTCTT
CGATTTGACA
AGTTAGATCA
AGCAGAAACC
AGGAGTCTCC
TGATGTGTTT
TCTTCACATT
ATGAAAACGA
TTTCGTACGA
GACTCTGATT
GCCTACTGGC
AGAGAGCTCC
CAGCCCAGAG
GTGTCCGACT
TCTTCAGCAT
CTTGGACGTT
TGTCTTCAAA
ATTTCAGATA
CCTAGGAAAA
CGCAACATAA
TAAGAAAAAT
TGCTGCTGCA
TTTAAATTTG
TGCAGTCCAC
CCAGAACAAT
GGAACTCTCA
AAGGATCCAA
TCCTTCCTGC
CAGACATCAG
GTCTCTTGTC
ACAATGGATG
CACTCCACTT
CCAGTCATGA
AGGCCATAAG
CAGCACAGAT
CACAAACTTT
ACCTATAATT
CGAGGCAAAA
ATGCACTTTC
TAACCCCAAA
CTCTTACAGA
TGTTAGACAA
TACCATGACC
AGCAACTCCA
ATGAAATAGA
CATCGTGCAT
CATGTAGACA
CTGAGGCCAA
TATAGCTACG
GAGGTTTGCC
TCACTATTCT
GGACCTGTTA
TCCCTCTTCA
CACAGACTCA
TCAGAGACAA
CTCCAACAAT
GTGACTGTCA
AAATTCATTG
ATGAAAAGGG
TCTTTGATAC
CTACATGCTC
TTTTTCCAAG
TCAATCAGTC
AGCCACAGCG
CTGAAGCATG
TGTTTGCCTC
CTTGAGACTG
TTTGTATGCA
AGTTTCCTTC
AGCGTTCTTT
CAAGGGCCTG
CTGACCTCTG
TGGATCATTC
TGTTACGAAG
TCCTAAAATG
CTGTAACCAC
AGGTCACGGT
CAACTCAGAC
AGAAGCCTCA
GCCCTTCTGT
ACTTTTTCAT
GAGGAAGAAT
TTTGAATTTA
ACAAGCAGAA
GTCAATTGTC
CGGTCCCACT
TACAACTCAT
CAAAGCTGTG
AGCCAGTTAA
TAGCCGATGC
TGGGATTGGA
GGCTCTCGGT
TTCAACATTC
GCTTGCAATT
ACCAATAATT
TCGCCAATGG
AGATAAGCAA
ATGAAAAACA
GCAATTTCCC
AGCAGAGGTG
AAGGACCTGA
CTAAGCGTAT
AAGGACTGTT
TTGATGGCCA
CAGCTCAGAT
GAAGGAGCAC
GAGAAGGACC
CCGAGATGCT
GGTGGATCTC
CAGTACAGCC
TGGCAAACTC
CTACGCAAGC
ACTCCTGAAC
TACGCAGGAC
CTGGCTGGAA
AGTATGGCCA
AGATTCATGC
CAGAAACGGA
GATTTATCTG
AGGCTGCCAT
GTGTTACATC
CATATTGCTG
CTCTCATTGC
AGAGTATCTG
AAAAGAAAGG
GTTACTGGAA
AGTGGAAAAG
ATTTGCACAG
CATCCCTGCT
CTCGGAGGAT
ACCCACCCCT
GTGATAGCAA
CTCATTACTA
ACAACTCCCA
GTGGAGGAAG
CATGTTCTCT
ATGGGATGGC
CAGCTTTTTT
GAGCATTACA
CCAAACATTA
AGGAAGAAGG
AGCGATGAAA
GAGGATTCTG
GAATGCAAGA
TACACCATAC
AATGAGAATA
TCCTGGTGGA
GCAGCTATAC
ATGTGTGTGG
AGTTTCTCTG
GAAGTCTGAG
CGATATGAAC
TCATTGCTGA
TGTCAACAAG
CCCATCATTG
CTGTCTTTGA
GAAACAACGA
GACTTCAAAA
AATTGTCAGA
TCTCTACTAC
GACATTCATC
GGTCATATCT
GAAAGTGGCA
GAGGTGGTGA
ATTCGGAGAA
GCGGCTGTTT
GGTCGCTACT
ATCGTGTGGC
ATTTTATGGG
CAGGGCTTTT
TTGAAGAAGA
AGAAGGTAAA
GAGTATATTT
ATAAAGAGCC
TAAGCTGACA
GGTCTGTCCG
AGATTTCCCA
AAGATTACTC
AAGCTCTATG
CAGATAAATT
TGGAGCAGAC
AATGTGAAGA
TAATCCAGGA
TTCCAACAAG
GTAAACCCCA
AGGATTTGGA
CCCCAAATAT
GCCTACATCC
AGGTGACCTA
TGTGACGCCG
TTCTTTGAGG
AAAAGGAAAT
CGAAGACCGG
AAGACAGATT
TCGAAATGCA
CCACAACATC
AACCGCTTTG
TCTTCGAGAC
ACCCTTCACG
CTGTCGGGCA
AGAAGCACGG
TGGGGTGGCG
GAGCAGTGCA
AGCGGCGGAC
GATCCTGACA
ACGAGTCACC
TGTTCCCCTA
CGTGAAGAAG
AGAATACAAG
TAATTAGCCA
ATCGAGCACA
GAACTGAATC
CAATTGAAGT
GGCAATTGAC
GAGATGTCCA
AGAAGGTTTC
TGAGCTTAAT
CAGCTTTGCA
CAATGGAAGA
AGTGGACATG
ATCAGACTGC
AGCTCAAACT
GCAAGGAAGT
GTCAGCGTGA
AGGTTAATGC
TGGGCCAATG
GCCTATGCAC
GAGCTTTTCT
TGAAGAAACC
AATGCAAAGA
AGTACCCTGA
CAACCAAGTA
AAGCTTTTGA
AGGAGATCTT
CAGGCAATTT
GCAGATGCAT
GTGGGCAGGC
CCTTGACGTG
AATGAGCGCC
TCATCAAAGA
GGACCAGCTG
GAGTACCAGG
AAGAACTGAG
GTCCCACTAC
AAGGACATGC
TCAGCGAACT
CTCCACAGTC
ATGAATGAGC
AGATTACGGG
CAGGGACGAC
CTGTCAAAGC
GCGGAGTGGA
CCAAACCTGC
ACTCGAGTAA
TTAGCAAAGC
AACTCCGGCC
CTACCCACGG
TCTCCATCTC
ATCTAGTGCT
GAAGTCTGGC
GGCCGCTCGA
GGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGACTACA
AGGATGACGA
TGACAAGTAG
TGAGGGCCCG
TTTAAACCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
TAGGGGGTAT
CCCCACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTAATTCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
AGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCTGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TCCCGGGAGC
TTGTATATCC
ATTTTCGGAT
CTGATCAAGA
GACAGGATGA
GGATCGTTTC
GCATGATTGA
ACAAGATGGA
TTGCACGCAG
GTTCTCCGGC
CGCTTGGGTG
GAGAGGCTAT
TCGGCTATGA
CTGGGCACAA
CAGACAATCG
GCTGCTCTGA
TGCCGCCGTG
TTCCGGCTGT
CAGCGCAGGG
GCGCCCGGTT
CTTTTTGTCA
AGACCGACCT
GTCCGGTGCC
CTGAATGAAC
TGCAGGACGA
GGCAGCGCGG
CTATCGTGGC
TGGCCACGAC
GGGCGTTCCT
TGCGCAGCTG
TGCTCGACGT
TGTCACTGAA
GCGGGAAGGG
ACTGGCTGCT
ATTGGGCGAA
GTGCCGGGGC
AGGATCTCCT
GTCATCTCAC
CTTGCTCCTG
CCGAGAAAGT
ATCCATCATG
GCTGATGCAA
TGCGGCGGCT
GCATACGCTT
GATCCGGCTA
CCTGCCCATT
CGACCACCAA
GCGAAACATC
GCATCGAGCG
AGCACGTACT
CGGATGGAAG
CCGGTCTTGT
CGATCAGGAT
GATCTGGACG
AAGAGCATCA
GGGGCTCGCG
CCAGCCGAAC
TGTTCGCCAG
GCTCAAGGCG
CGCATGCCCG
ACGGCGAGGA
TCTCGTCGTG
ACCCATGGCG
ATGCCTGCTT
GCCGAATATC
ATGGTGGAAA
ATGGCCGCTT
TTCTGGATTC
ATCGACTGTG
GCCGGCTGGG
TGTGGCGGAC
CGCTATCAGG
ACATAGCGTT
GGCTACCCGT
GATATTGCTG
AAGAGCTTGG
CGGCGAATGG
GCTGACCGCT
TCCTCGTGCT
TTACGGTATC
GCCGCTCCCG
ATTCGCAGCG
CATCGCCTTC
TATCGCCTTC
TTGACGAGTT
CTTCTGAGCG
GGACTCTGGG
GTTCGAAATG
ACCGACCAAG
CGACGCCCAA
CCTGCCATCA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GTATACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTC