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pLV3-CMV-POSTN(human)-3×FLAG-CopGFP-Puro

产品货号:P67118

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV3-CMV-POSTN(human)-3×FLAG-CopGFP-Puro
别称: NM_006475.3;OSF-2; OSF2; PDLPOSTN; PN
基因长: 2508bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记: Puro
荧光标记: 绿色
启动子: CMV
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ACGCGTGTAG

TCTTATGCAA

TACTCTTGTA

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

61

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

121

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

181

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

GTGCCTAGCT

CGATACAATA

AACGGGTCTC

241

TCTGGTTAGA

CCAGATCTGA

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

301

AGCCTCAATA

AAGCTTGCCT

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

361

CTGGTAACTA

GAGATCCCTC

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

421

CCCGAACAGG

GACCTGAAAG

CGAAAGGGAA

ACCAGAGCTC

TCTCGACGCA

GGACTCGGCT

481

TGCTGAAGCG

CGCACGGCAA

GAGGCGAGGG

GCGGCGACTG

GTGAGTACGC

CAAAAATTTT

541

GACTAGCGGA

GGCTAGAAGG

AGAGAGATGG

GTGCGAGAGC

GTCAGTATTA

AGCGGGGGAG

601

AATTAGATCG

CGATGGGAAA

AAATTCGGTT

AAGGCCAGGG

GGAAAGAAAA

AATATAAATT

661

AAAACATATA

GTATGGGCAA

GCAGGGAGCT

AGAACGATTC

GCAGTTAATC

CTGGCCTGTT

721

AGAAACATCA

GAAGGCTGTA

GACAAATACT

GGGACAGCTA

CAACCATCCC

TTCAGACAGG

781

ATCAGAAGAA

CTTAGATCAT

TATATAATAC

AGTAGCAACC

CTCTATTGTG

TGCATCAAAG

841

GATAGAGATA

AAAGACACCA

AGGAAGCTTT

AGACAAGATA

GAGGAAGAGC

AAAACAAAAG

901

TAAGACCACC

GCACAGCAAG

CGGCCACTGA

TCTTCAGACC

TGGAGGAGGA

GATATGAGGG

961

ACAATTGGAG

AAGTGAATTA

TATAAATATA

AAGTAGTAAA

AATTGAACCA

TTAGGAGTAG

1021

CACCCACCAA

GGCAAAGAGA

AGAGTGGTGC

AGAGAGAAAA

AAGAGCAGTG

GGAATAGGAG

1081

CTTTGTTCCT

TGGGTTCTTG

GGAGCAGCAG

GAAGCACTAT

GGGCGCAGCC

TCAATGACGC

1141

TGACGGTACA

GGCCAGACAA

TTATTGTCTG

GTATAGTGCA

GCAGCAGAAC

AATTTGCTGA

1201

GGGCTATTGA

GGCGCAACAG

CATCTGTTGC

AACTCACAGT

CTGGGGCATC

AAGCAGCTCC

1261

AGGCAAGAAT

CCTGGCTGTG

GAAAGATACC

TAAAGGATCA

ACAGCTCCTG

GGGATTTGGG

1321

GTTGCTCTGG

AAAACTCATT

TGCACCACTG

CTGTGCCTTG

GAATGCTAGT

TGGAGTAATA

1381

AATCTCTGGA

ACAGATTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

1441

TTACACAAGC

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

1501

ACAAGAATTA

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

1561

TTGGCTGTGG

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

1621

AGTTTTTGCT

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

1681

TCAGACCCAC

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

1741

TGGAGAGAGA

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGGTT

1801

AACTTTTAAA

AGAAAAGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

1861

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAA

TTCAAAATTT

1921

TATCGATACT

AGTATTATGC

CCAGTACATG

ACCTTATGGG

ACTTTCCTAC

TTGGCAGTAC

1981

ATCTACGTAT

TAGTCATCGC

TATTACCATG

GTGATGCGGT

TTTGGCAGTA

CATCAATGGG

2041

CGTGGATAGC

GGTTTGACTC

ACGGGGATTT

CCAAGTCTCC

ACCCCATTGA

CGTCAATGGG

2101

AGTTTGTTTT

GGCACCAAAA

TCAACGGGAC

TTTCCAAAAT

GTCGTAACAA

CTCCGCCCCA

2161

TTGACGCAAA

TGGGCGGTAG

GCGTGTACGG

TGGGAGGTTT

ATATAAGCAG

AGCTCGTTTA

2221

GTGAACCGTC

AGATCGCCTG

GAGACGCCAT

CCACGCTGTT

TTGACCTCCA

TAGAAGATTC

2281

TAGAGCTAGC

CTGATACGAA

CTCGGAATTC

GCCACCATGA

TTCCCTTTTT

ACCCATGTTT

2341

TCTCTACTAT

TGCTGCTTAT

TGTTAACCCT

ATAAACGCCA

ACAATCATTA

TGACAAGATC

2401

TTGGCTCATA

GTCGTATCAG

GGGTCGGGAC

CAAGGCCCAA

ATGTCTGTGC

CCTTCAACAG

2461

ATTTTGGGCA

CCAAAAAGAA

ATACTTCAGC

ACTTGTAAGA

ACTGGTATAA

AAAGTCCATC

2521

TGTGGACAGA

AAACGACTGT

GTTATATGAA

TGTTGCCCTG

GTTATATGAG

AATGGAAGGA

2581

ATGAAAGGCT

GCCCAGCAGT

TTTGCCCATT

GACCATGTTT

ATGGCACTCT

GGGCATCGTG

2641

GGAGCCACCA

CAACGCAGCG

CTATTCTGAC

GCCTCAAAAC

TGAGGGAGGA

GATCGAGGGA

2701

AAGGGATCCT

TCACTTACTT

TGCACCGAGT

AATGAGGCTT

GGGACAACTT

GGATTCTGAT

2761

ATCCGTAGAG

GTTTGGAGAG

CAACGTGAAT

GTTGAATTAC

TGAATGCTTT

ACATAGTCAC

2821

ATGATTAATA

AGAGAATGTT

GACCAAGGAC

TTAAAAAATG

GCATGATTAT

TCCTTCAATG

2881

TATAACAATT

TGGGGCTTTT

CATTAACCAT

TATCCTAATG

GGGTTGTCAC

TGTTAATTGT

2941

GCTCGAATCA

TCCATGGGAA

CCAGATTGCA

ACAAATGGTG

TTGTCCATGT

CATTGACCGT

3001

GTGCTTACAC

AAATTGGTAC

CTCAATTCAA

GACTTCATTG

AAGCAGAAGA

TGACCTTTCA

3061

TCTTTTAGAG

CAGCTGCCAT

CACATCGGAC

ATATTGGAGG

CCCTTGGAAG

AGACGGTCAC

3121

TTCACACTCT

TTGCTCCCAC

CAATGAGGCT

TTTGAGAAAC

TTCCACGAGG

TGTCCTAGAA

3181

AGGATCATGG

GAGACAAAGT

GGCTTCCGAA

GCTCTTATGA

AGTACCACAT

CTTAAATACT

3241

CTCCAGTGTT

CTGAGTCTAT

TATGGGAGGA

GCAGTCTTTG

AGACGCTGGA

AGGAAATACA

3301

ATTGAGATAG

GATGTGACGG

TGACAGTATA

ACAGTAAATG

GAATCAAAAT

GGTGAACAAA

3361

AAGGATATTG

TGACAAATAA

TGGTGTGATC

CATTTGATTG

ATCAGGTCCT

AATTCCTGAT

3421

TCTGCCAAAC

AAGTTATTGA

GCTGGCTGGA

AAACAGCAAA

CCACCTTCAC

GGATCTTGTG

3481

GCCCAATTAG

GCTTGGCATC

TGCTCTGAGG

CCAGATGGAG

AATACACTTT

GCTGGCACCT

3541

GTGAATAATG

CATTTTCTGA

TGATACTCTC

AGCATGGATC

AGCGCCTCCT

TAAATTAATT

3601

CTGCAGAATC

ACATATTGAA

AGTAAAAGTT

GGCCTTAATG

AGCTTTACAA

CGGGCAAATA

3661

CTGGAAACCA

TCGGAGGCAA

ACAGCTCAGA

GTCTTCGTAT

ATCGTACAGC

TGTCTGCATT

3721

GAAAATTCAT

GCATGGAGAA

AGGGAGTAAG

CAAGGGAGAA

ACGGTGCGAT

TCACATATTC

3781

CGCGAGATCA

TCAAGCCAGC

AGAGAAATCC

CTCCATGAAA

AGTTAAAACA

AGATAAGCGC

3841

TTTAGCACCT

TCCTCAGCCT

ACTTGAAGCT

GCAGACTTGA

AAGAGCTCCT

GACACAACCT

3901

GGAGACTGGA

CATTATTTGT

GCCAACCAAT

GATGCTTTTA

AGGGAATGAC

TAGTGAAGAA

3961

AAAGAAATTC

TGATACGGGA

CAAAAATGCT

CTTCAAAACA

TCATTCTTTA

TCACCTGACA

4021

CCAGGAGTTT

TCATTGGAAA

AGGATTTGAA

CCTGGTGTTA

CTAACATTTT

AAAGACCACA

4081

CAAGGAAGCA

AAATCTTTCT

GAAAGAAGTA

AATGATACAC

TTCTGGTGAA

TGAATTGAAA

4141

TCAAAAGAAT

CTGACATCAT

GACAACAAAT

GGTGTAATTC

ATGTTGTAGA

TAAACTCCTC

4201

TATCCAGCAG

ACACACCTGT

TGGAAATGAT

CAACTGCTGG

AAATACTTAA

TAAATTAATC

4261

AAATACATCC

AAATTAAGTT

TGTTCGTGGT

AGCACCTTCA

AAGAAATCCC

CGTGACTGTC

4321

TATACAACTA

AAATTATAAC

CAAAGTTGTG

GAACCAAAAA

TTAAAGTGAT

TGAAGGCAGT

4381

CTTCAGCCTA

TTATCAAAAC

TGAAGGACCC

ACACTAACAA

AAGTCAAAAT

TGAAGGTGAA

4441

CCTGAATTCA

GACTGATTAA

AGAAGGTGAA

ACAATAACTG

AAGTGATCCA

TGGAGAGCCA

4501

ATTATTAAAA

AATACACCAA

AATCATTGAT

GGAGTGCCTG

TGGAAATAAC

TGAAAAAGAG

4561

ACACGAGAAG

AACGAATCAT

TACAGGTCCT

GAAATAAAAT

ACACTAGGAT

TTCTACTGGA

4621

GGTGGAGAAA

CAGAAGAAAC

TCTGAAGAAA

TTGTTACAAG

AAGAGGTCAC

CAAGGTCACC

4681

AAATTCATTG

AAGGTGGTGA

TGGTCATTTA

TTTGAAGATG

AAGAAATTAA

AAGACTGCTT

4741

CAGGGAGACA

CACCCGTGAG

GAAGTTGCAA

GCCAACAAAA

AAGTTCAAGG

ATCTAGAAGA

4801

CGATTAAGGG

AAGGTCGTTC

TCAGGGATCC

GACTACAAAG

ACCATGACGG

TGATTATAAA

4861

GATCATGACA

TCGACTACAA

GGATGACGAT

GACAAGTAGC

GGCCGCGAAG

GATCTGCGAT

4921

CGCTCCGGTG

CCCGTCAGTG

GGCAGAGCGC

ACATCGCCCA

CAGTCCCCGA

GAAGTTGGGG

4981

GGAGGGGTCG

GCAATTGAAC

GGGTGCCTAG

AGAAGGTGGC

GCGGGGTAAA

CTGGGAAAGT

5041

GATGTCGTGT

ACTGGCTCCG

CCTTTTTCCC

GAGGGTGGGG

GAGAACCGTA

TATAAGTGCA

5101

GTAGTCGCCG

TGAACGTTCT

TTTTCGCAAC

GGGTTTGCCG

CCAGAACACA

GCTGAAGCTT

5161

CGAGGGGCTC

GCATCTCTCC

TTCACGCGCC

CGCCGCCCTA

CCTGAGGCCG

CCATCCACGC

5221

CGGTTGAGTC

GCGTTCTGCC

GCCTCCCGCC

TGTGGTGCCT

CCTGAACTGC

GTCCGCCGTC

5281

TAGGTAAGTT

TAAAGCTCAG

GTCGAGACCG

GGCCTTTGTC

CGGCGCTCCC

TTGGAGCCTA

5341

CCTAGACTCA

GCCGGCTCTC

CACGCTTTGC

CTGACCCTGC

TTGCTCAACT

CTACGTCTTT

5401

GTTTCGTTTT

CTGTTCTGCG

CCGTTACAGA

TCCAAGCTGT

GACCGGCGCC

TACGCTAGAC

5461

GCCACCATGG

AGAGCGACGA

GAGCGGCCTG

CCCGCCATGG

AGATCGAGTG

CCGCATCACC

5521

GGCACCCTGA

ACGGCGTGGA

GTTCGAGCTG

GTGGGCGGCG

GAGAGGGCAC

CCCCAAGCAG

5581

GGCCGCATGA

CCAACAAGAT

GAAGAGCACC

AAAGGCGCCC

TGACCTTCAG

CCCCTACCTG

5641

CTGAGCCACG

TGATGGGCTA

CGGCTTCTAC

CACTTCGGCA

CCTACCCCAG

CGGCTACGAG

5701

AACCCCTTCC

TGCACGCCAT

CAACAACGGC

GGCTACACCA

ACACCCGCAT

CGAGAAGTAC

5761

GAGGACGGCG

GCGTGCTGCA

CGTGAGCTTC

AGCTACCGCT

ACGAGGCCGG

CCGCGTGATC

5821

GGCGACTTCA

AGGTGGTGGG

CACCGGCTTC

CCCGAGGACA

GCGTGATCTT

CACCGACAAG

5881

ATCATCCGCA

GCAACGCCAC

CGTGGAGCAC

CTGCACCCCA

TGGGCGATAA

CGTGCTGGTG

5941

GGCAGCTTCG

CCCGCACCTT

CAGCCTGCGC

GACGGCGGCT

ACTACAGCTT

CGTGGTGGAC

6001

AGCCACATGC

ACTTCAAGAG

CGCCATCCAC

CCCAGCATCC

TGCAGAACGG

GGGCCCCATG

6061

TTCGCCTTCC

GCCGCGTGGA

GGAGCTGCAC

AGCAACACCG

AGCTGGGCAT

CGTGGAGTAC

6121

CAGCACGCCT

TCAAGACCCC

CATCGCCTTC

GCCAGATCCC

GCGCTCAGTC

GTCCAATTCT

6181

GCCGTGGACG

GCACCGCCGG

ACCCGGCTCC

ACCGGATCTC

GCGAGGGCAG

AGGAAGTCTT

6241

CTAACATGCG

GTGACGTGGA

GGAGAATCCC

GGCCCTATGA

CCGAGTACAA

GCCCACGGTG

6301

CGCCTCGCCA

CCCGCGACGA

CGTCCCCAGG

GCCGTACGCA

CCCTCGCCGC

CGCGTTCGCC

6361

GACTACCCCG

CCACGCGCCA

CACCGTCGAT

CCGGACCGCC

ACATCGAGCG

GGTCACCGAG

6421

CTGCAAGAAC

TCTTCCTCAC

GCGCGTCGGG

CTCGACATCG

GCAAGGTGTG

GGTCGCGGAC

6481

GACGGCGCCG

CGGTGGCGGT

CTGGACCACG

CCGGAGAGCG

TCGAAGCGGG

GGCGGTGTTC

6541

GCCGAGATCG

GCCCGCGCAT

GGCCGAGTTG

AGCGGTTCCC

GGCTGGCCGC

GCAGCAACAG

6601

ATGGAAGGCC

TCCTGGCGCC

GCACCGGCCC

AAGGAGCCCG

CGTGGTTCCT

GGCCACCGTC

6661

GGCGTCTCGC

CCGACCACCA

GGGCAAGGGT

CTGGGCAGCG

CCGTCGTGCT

CCCCGGAGTG

6721

GAGGCGGCCG

AGCGCGCCGG

GGTGCCCGCC

TTCCTGGAGA

CCTCCGCGCC

CCGCAACCTC

6781

CCCTTCTACG

AGCGGCTCGG

CTTCACCGTC

ACCGCCGACG

TCGAGGTGCC

CGAAGGACCG

6841

CGCACCTGGT

GCATGACCCG

CAAGCCCGGT

GCCTGAAATC

AACCTCTGGA

TTACAAAATT

6901

TGTGAAAGAT

TGACTGGTAT

TCTTAACTAT

GTTGCTCCTT

TTACGCTATG

TGGATACGCT

6961

GCTTTAATGC

CTTTGTATCA

TGCTATTGCT

TCCCGTATGG

CTTTCATTTT

CTCCTCCTTG

7021

TATAAATCCT

GGTTGCTGTC

TCTTTATGAG

GAGTTGTGGC

CCGTTGTCAG

GCAACGTGGC

7081

GTGGTGTGCA

CTGTGTTTGC

TGACGCAACC

CCCACTGGTT

GGGGCATTGC

CACCACCTGT

7141

CAGCTCCTTT

CCGGGACTTT

CGCTTTCCCC

CTCCCTATTG

CCACGGCGGA

ACTCATCGCC

7201

GCCTGCCTTG

CCCGCTGCTG

GACAGGGGCT

CGGCTGTTGG

GCACTGACAA

TTCCGTGGTG

7261

TTGTCGGGGA

AATCATCGTC

CTTTCCTTGG

CTGCTCGCCT

GTGTTGCCAC

CTGGATTCTG

7321

CGCGGGACGT

CCTTCTGCTA

CGTCCCTTCG

GCCCTCAATC

CAGCGGACCT

TCCTTCCCGC

7381

GGCCTGCTGC

CGGCTCTGCG

GCCTCTTCCG

CGTCTTCGCC

TTCGCCCTCA

GACGAGTCGG

7441

ATCTCCCTTT

GGGCCGCCTC

CCCGCCTGGT

ACCTTTAAGA

CCAATGACTT

ACAAGGCAGC

7501

TGTAGATCTT

AGCCACTTTT

TAAAAGAAAA

GGGGGGACTG

GAAGGGCTAA

TTCACTCCCA

7561

ACGAAGATAA

GATCTGCTTT

TTGCTTGTAC

TGGGTCTCTC

TGGTTAGACC

AGATCTGAGC

7621

CTGGGAGCTC

TCTGGCTAAC

TAGGGAACCC

ACTGCTTAAG

CCTCAATAAA

GCTTGCCTTG

7681

AGTGCTTCAA

GTAGTGTGTG

CCCGTCTGTT

GTGTGACTCT

GGTAACTAGA

GATCCCTCAG

7741

ACCCTTTTAG

TCAGTGTGGA

AAATCTCTAG

CAGTAGTAGT

TCATGTCATC

TTATTATTCA

7801

GTATTTATAA

CTTGCAAAGA

AATGAATATC

AGAGAGTGAG

AGGAACTTGT

TTATTGCAGC

7861

TTATAATGGT

TACAAATAAA

GCAATAGCAT

CACAAATTTC

ACAAATAAAG

CATTTTTTTC

7921

ACTGCATTCT

AGTTGTGGTT

TGTCCAAACT

CATCAATGTA

TCTTATCATG

TCTGGCTCTA

7981

GCTATCCCGC

CCCTAACTCC

GCCCATCCCG

CCCCTAACTC

CGCCCAGTTC

CGCCCATTCT

8041

CCGCCCCATG

GCTGACTAAT

TTTTTTTATT

TATGCAGAGG

CCGAGGCCGC

CTCGGCCTCT

8101

GAGCTATTCC

AGAAGTAGTG

AGGAGGCTTT

TTTGGAGGCC

TAGACTTTTG

CAGAGACCAA

8161

ATTCGTAATC

ATGTCATAGC

TGTTTCCTGT

GTGAAATTGT

TATCCGCTCA

CAATTCCACA

8221

CAACATACGA

GCCGGAAGCA

TAAAGTGTAA

AGCCTGGGGT

GCCTAATGAG

TGAGCTAACT

8281

CACATTAATT

GCGTTGCGCT

CACTGCCCGC

TTTCCAGTCG

GGAAACCTGT

CGTGCCAGCT

8341

GCATTAATGA

ATCGGCCAAC

GCGCGGGGAG

AGGCGGTTTG

CGTATTGGGC

GCTCTTCCGC

8401

TTCCTCGCTC

ACTGACTCGC

TGCGCTCGGT

CGTTCGGCTG

CGGCGAGCGG

TATCAGCTCA

8461

CTCAAAGGCG

GTAATACGGT

TATCCACAGA

ATCAGGGGAT

AACGCAGGAA

AGAACATGTG

8521

AGCAAAAGGC

CAGCAAAAGG

CCAGGAACCG

TAAAAAGGCC

GCGTTGCTGG

CGTTTTTCCA

8581

TAGGCTCCGC

CCCCCTGACG

AGCATCACAA

AAATCGACGC

TCAAGTCAGA

GGTGGCGAAA

8641

CCCGACAGGA

CTATAAAGAT

ACCAGGCGTT

TCCCCCTGGA

AGCTCCCTCG

TGCGCTCTCC

8701

TGTTCCGACC

CTGCCGCTTA

CCGGATACCT

GTCCGCCTTT

CTCCCTTCGG

GAAGCGTGGC

8761

GCTTTCTCAT

AGCTCACGCT

GTAGGTATCT

CAGTTCGGTG

TAGGTCGTTC

GCTCCAAGCT

8821

GGGCTGTGTG

CACGAACCCC

CCGTTCAGCC

CGACCGCTGC

GCCTTATCCG

GTAACTATCG

8881

TCTTGAGTCC

AACCCGGTAA

GACACGACTT

ATCGCCACTG

GCAGCAGCCA

CTGGTAACAG

8941

GATTAGCAGA

GCGAGGTATG

TAGGCGGTGC

TACAGAGTTC

TTGAAGTGGT

GGCCTAACTA

9001

CGGCTACACT

AGAAGGACAG

TATTTGGTAT

CTGCGCTCTG

CTGAAGCCAG

TTACCTTCGG

9061

AAAAAGAGTT

GGTAGCTCTT

GATCCGGCAA

ACAAACCACC

GCTGGTAGCG

GTGGTTTTTT

9121

TGTTTGCAAG

CAGCAGATTA

CGCGCAGAAA

AAAAGGATCT

CAAGAAGATC

CTTTGATCTT

9181

TTCTACGGGG

TCTGACGCTC

AGTGGAACGA

AAACTCACGT

TAAGGGATTT

TGGTCATGAG

9241

ATTATCAAAA

AGGATCTTCA

CCTAGATCCT

TTTAAATTAA

AAATGAAGTT

TTAAATCAAT

9301

CTAAAGTATA

TATGAGTAAA

CTTGGTCTGA

CAGTTACCAA

TGCTTAATCA

GTGAGGCACC

9361

TATCTCAGCG

ATCTGTCTAT

TTCGTTCATC

CATAGTTGCC

TGACTCCCCG

TCGTGTAGAT

9421

AACTACGATA

CGGGAGGGCT

TACCATCTGG

CCCCAGTGCT

GCAATGATAC

CGCGAGACCC

9481

ACGCTCACCG

GCTCCAGATT

TATCAGCAAT

AAACCAGCCA

GCCGGAAGGG

CCGAGCGCAG

9541

AAGTGGTCCT

GCAACTTTAT

CCGCCTCCAT

CCAGTCTATT

AATTGTTGCC

GGGAAGCTAG

9601

AGTAAGTAGT

TCGCCAGTTA

ATAGTTTGCG

CAACGTTGTT

GCCATTGCTA

CAGGCATCGT

9661

GGTGTCACGC

TCGTCGTTTG

GTATGGCTTC

ATTCAGCTCC

GGTTCCCAAC

GATCAAGGCG

9721

AGTTACATGA

TCCCCCATGT

TGTGCAAAAA

AGCGGTTAGC

TCCTTCGGTC

CTCCGATCGT

9781

TGTCAGAAGT

AAGTTGGCCG

CAGTGTTATC

ACTCATGGTT

ATGGCAGCAC

TGCATAATTC

9841

TCTTACTGTC

ATGCCATCCG

TAAGATGCTT

TTCTGTGACT

GGTGAGTACT

CAACCAAGTC

9901

ATTCTGAGAA

TAGTGTATGC

GGCGACCGAG

TTGCTCTTGC

CCGGCGTCAA

TACGGGATAA

9961

TACCGCGCCA

CATAGCAGAA

CTTTAAAAGT

GCTCATCATT

GGAAAACGTT

CTTCGGGGCG

10021

AAAACTCTCA

AGGATCTTAC

CGCTGTTGAG

ATCCAGTTCG

ATGTAACCCA

CTCGTGCACC

10081

CAACTGATCT

TCAGCATCTT

TTACTTTCAC

CAGCGTTTCT

GGGTGAGCAA

AAACAGGAAG

10141

GCAAAATGCC

GCAAAAAAGG

GAATAAGGGC

GACACGGAAA

TGTTGAATAC

TCATACTCTT

10201

CCTTTTTCAA

TATTATTGAA

GCATTTATCA

GGGTTATTGT

CTCATGAGCG

GATACATATT

10261

TGAATGTATT

TAGAAAAATA

AACAAATAGG

GGTTCCGCGC

ACATTTCCCC

GAAAAGTGCC

10321

ACCTGACGTC

TAAGAAACCA

TTATTATCAT

GACATTAACC

TATAAAAATA

GGCGTATCAC

10381

GAGGCCCTTT

CGTCTCGCGC

GTTTCGGTGA

TGACGGTGAA

AACCTCTGAC

ACATGCAGCT

10441

CCCGGAGACG

GTCACAGCTT

GTCTGTAAGC

GGATGCCGGG

AGCAGACAAG

CCCGTCAGGG

10501

CGCGTCAGCG

GGTGTTGGCG

GGTGTCGGGG

CTGGCTTAAC

TATGCGGCAT

CAGAGCAGAT

10561

TGTACTGAGA

GTGCACCATA

TGCGGTGTGA

AATACCGCAC

AGATGCGTAA

GGAGAAAATA

10621

CCGCATCAGG

CGCCATTCGC

CATTCAGGCT

GCGCAACTGT

TGGGAAGGGC

GATCGGTGCG

10681

GGCCTCTTCG

CTATTACGCC

AGCTGGCGAA

AGGGGGATGT

GCTGCAAGGC

GATTAAGTTG

10741

GGTAACGCCA

GGGTTTTCCC

AGTCACGACG

TTGTAAAACG

ACGGCCAGTG

CCAAGCTG

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