名称: | pHIV-EF1a-CCBE1(human)-IRES2-EGFP |
---|---|
别称: | NM_133459.4;HKLLS1; |
基因长: | 1221bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pHIV-EGFP |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
GCCGCTGATC
TTCAGACCTG
GAGGAGGAGA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCGGCACT
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGTTA
GTACCGGGCC
CGCTCTAGCG
TGAGGCTCCG
GTGCCCGTCA
GTGGGCAGAG
CGCACATCGC
CCACAGTCCC
CGAGAAGTTG
GGGGGAGGGG
TCGGCAATTG
AACCGGTGCC
TAGAGAAGGT
GGCGCGGGGT
AAACTGGGAA
AGTGATGTCG
TGTACTGGCT
CCGCCTTTTT
CCCGAGGGTG
GGGGAGAACC
GTATATAAGT
GCAGTAGTCG
CCGTGAACGT
TCTTTTTCGC
AACGGGTTTG
CCGCCAGAAC
ACAGGTAAGT
GCCGTGTGTG
GTTCCCGCGG
GCCTGGCCTC
TTTACGGGTT
ATGGCCCTTG
CGTGCCTTGA
ATTACTTCCA
CCTGGCTGCA
GTACGTGATT
CTTGATCCCG
AGCTTCGGGT
TGGAAGTGGG
TGGGAGAGTT
CGAGGCCTTG
CGCTTAAGGA
GCCCCTTCGC
CTCGTGCTTG
AGTTGAGGCC
TGGCCTGGGC
GCTGGGGCCG
CCGCGTGCGA
ATCTGGTGGC
ACCTTCGCGC
CTGTCTCGCT
GCTTTCGATA
AGTCTCTAGC
CATTTAAAAT
TTTTGATGAC
CTGCTGCGAC
GCTTTTTTTC
TGGCAAGATA
GTCTTGTAAA
TGCGGGCCAA
GATCTGCACA
CTGGTATTTC
GGTTTTTGGG
GCCGCGGGCG
GCGACGGGGC
CCGTGCGTCC
CAGCGCACAT
GTTCGGCGAG
GCGGGGCCTG
CGAGCGCGGC
CACCGAGAAT
CGGACGGGGG
TAGTCTCAAG
CTGGCCGGCC
TGCTCTGGTG
CCTGGCCTCG
CGCCGCCGTG
TATCGCCCCG
CCCTGGGCGG
CAAGGCTGGC
CCGGTCGGCA
CCAGTTGCGT
GAGCGGAAAG
ATGGCCGCTT
CCCGGCCCTG
CTGCAGGGAG
CTCAAAATGG
AGGACGCGGC
GCTCGGGAGA
GCGGGCGGGT
GAGTCACCCA
CACAAAGGAA
AAGGGCCTTT
CCGTCCTCAG
CCGTCGCTTC
ATGTGACTCC
ACGGAGTACC
GGGCGCCGTC
CAGGCACCTC
GATTAGTTCT
CGAGCTTTTG
GAGTACGTCG
TCTTTAGGTT
GGGGGGAGGG
GTTTTATGCG
ATGGAGTTTC
CCCACACTGA
GTGGGTGGAG
ACTGAAGTTA
GGCCAGCTTG
GCACTTGATG
TAATTCTCCT
TGGAATTTGC
CCTTTTTGAG
TTTGGATCTT
GGTTCATTCT
CAAGCCTCAG
ACAGTGGTTC
AAAGTTTTTT
TCTTCCATTT
CAGGTGTCGT
GAGCGGCCGC
TGAGTTAACT
ATTCTAGAGC
CACCATGGTG
CCGCCGCCTC
CGAGCCGGGG
AGGAGCTGCC
AGGGGCCAGC
TGGGCAGGAG
CCTGGGTCCG
CTGCTGCTGC
TCCTGGCGTT
GGGACACACG
TGGACCTACA
GAGAGGAGCC
GGAGGACGGC
GACAGAGAAA
TCTGCTCAGA
GAGCAAAATC
GCGACGACTA
AATACCCGTG
TCTGAAGTCT
TCAGGCGAGC
TCACCACATG
CTACAGGAAA
AAGTGCTGCA
AAGGATATAA
ATTTGTTCTT
GGACAATGCA
TCCCAGAAGA
TTACGACGTT
TGTGCCGAGG
CTCCCTGTGA
ACAGCAGTGC
ACGGACAACT
TTGGCCGAGT
GCTGTGTACT
TGTTATCCGG
GATACCGATA
TGACCGGGAG
AGACACCGGA
AGCGGGAGAA
GCCATACTGT
CTGGATATTG
ATGAGTGTGC
CAGCAGCAAT
GGGACGCTGT
GTGCCCACAT
CTGCATCAAT
ACCTTGGGCA
GCTACCGCTG
CGAGTGCCGG
GAAGGCTACA
TCCGGGAAGA
TGATGGGAAG
ACATGTACCA
GGGGAGACAA
ATATCCCAAT
GACACTGGCC
ATGAGAAGTC
TGAGAACATG
GTGAAAGCCG
GAACTTGCTG
TGCCACATGC
AAGGAGTTCT
ACCAGATGAA
GCAGACCGTG
CTGCAGCTGA
AGCAAAAGAT
TGCTCTGCTC
CCCAACAATG
CAGCTGACCT
GGGCAAGTAT
ATCACTGGTG
ACAAGGTGCT
GGCCTCAAAC
ACCTACCTTC
CAGGACCTCC
TGGCCTGCCT
GGGGGCCAGG
GCCCTCCCGG
CTCACCAGGA
CCAAAGGGAA
GCCCAGGCTT
CCCCGGTATG
CCAGGCCCTC
CTGGGCAGCC
CGGCCCACGG
GGCTCAATGG
GACCCATGGG
ACCATCTCCT
GATCTGTCCC
ACATTAAGCA
AGGCCGGAGG
GGCCCTGTGG
GTCCACCAGG
GGCACCAGGA
AGAGATGGTT
CTAAGGGGGA
GAGAGGAGCG
CCTGGGCCCA
GAGGGTCTCC
AGGACCCCCT
GGTTCTTTCG
ACTTCCTGCT
ACTTATGCTG
GCTGACATCC
GCAATGACAT
CACTGAGCTG
CAGGAAAAGG
TGTTCGGGCA
CCGGACTCAC
TCTTCAGCAG
AGGAGTTCCC
TTTACCTCAG
GAATTTCCCA
GCTACCCAGA
AGCCATGGAC
CTGGGCTCTG
GAGATGACCA
TCCAAGAAGA
ACTGAGACAA
GAGACTTGAG
AGCCCCCAGA
GACTTCTACC
CATAGGGATC
CGCCCCTCTC
CCTCCCCCCC
CCCTAACGTT
ACTGGCCGAA
GCCGCTTGGA
ATAAGGCCGG
TGTGCGTTTG
TCTATATGTT
ATTTTCCACC
ATATTGCCGT
CTTTTGGCAA
TGTGAGGGCC
CGGAAACCTG
GCCCTGTCTT
CTTGACGAGC
ATTCCTAGGG
GTCTTTCCCC
TCTCGCCAAA
GGAATGCAAG
GTCTGTTGAA
TGTCGTGAAG
GAAGCAGTTC
CTCTGGAAGC
TTCTTGAAGA
CAAACAACGT
CTGTAGCGAC
CCTTTGCAGG
CAGCGGAACC
CCCCACCTGG
CGACAGGTGC
CTCTGCGGCC
AAAAGCCACG
TGTATAAGAT
ACACCTGCAA
AGGCGGCACA
ACCCCAGTGC
CACGTTGTGA
GTTGGATAGT
TGTGGAAAGA
GTCAAATGGC
TCTCCTCAAG
CGTATTCAAC
AAGGGGCTGA
AGGATGCCCA
GAAGGTACCC
CATTGTATGG
GATCTGATCT
GGGGCCTCGG
TACACATGCT
TTACATGTGT
TTAGTCGAGG
TTAAAAAAAC
GTCTAGGCCC
CCCGAACCAC
GGGGACGTGG
TTTTCCTTTG
AAAAACACGA
TGATAATATG
GCCACAACCA
TGGTGAGCAA
GGGCGAGGAG
CTGTTCACCG
GGGTGGTGCC
CATCCTGGTC
GAGCTGGACG
GCGACGTAAA
CGGCCACAAG
TTCAGCGTGT
CCGGCGAGGG
CGAGGGCGAT
GCCACCTACG
GCAAGCTGAC
CCTGAAGTTC
ATCTGCACCA
CCGGCAAGCT
GCCCGTGCCC
TGGCCCACCC
TCGTGACCAC
CCTGACCTAC
GGCGTGCAGT
GCTTCAGCCG
CTACCCCGAC
CACATGAAGC
AGCACGACTT
CTTCAAGTCC
GCCATGCCCG
AAGGCTACGT
CCAGGAGCGC
ACCATCTTCT
TCAAGGACGA
CGGCAACTAC
AAGACCCGCG
CCGAGGTGAA
GTTCGAGGGC
GACACCCTGG
TGAACCGCAT
CGAGCTGAAG
GGCATCGACT
TCAAGGAGGA
CGGCAACATC
CTGGGGCACA
AGCTGGAGTA
CAACTACAAC
AGCCACAACG
TCTATATCAT
GGCCGACAAG
CAGAAGAACG
GCATCAAGGT
GAACTTCAAG
ATCCGCCACA
ACATCGAGGA
CGGCAGCGTG
CAGCTCGCCG
ACCACTACCA
GCAGAACACC
CCCATCGGCG
ACGGCCCCGT
GCTGCTGCCC
GACAACCACT
ACCTGAGCAC
CCAGTCCGCC
CTGAGCAAAG
ACCCCAACGA
GAAGCGCGAT
CACATGGTCC
TGCTGGAGTT
CGTGACCGCC
GCCGGGATCA
CTCTCGGCAT
GGACGAGCTG
TACAAGTAAA
GCGGCCGCAT
CGATACCGTC
GACCTCGATC
GAGACCTAGA
AAAACATGGA
GCAATCACAA
GTAGCAATAC
AGCAGCTACC
AATGCTGATT
GTGCCTGGCT
AGAAGCACAA
GAGGAGGAGG
AGGTGGGTTT
TCCAGTCACA
CCTCAGGTAC
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTGGC
AGAACTACAC
ACCAGGGCCA
GGGATCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCAAGAG
AAGGTAGAAG
AAGCCAATGA
AGGAGAGAAC
ACCCGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTATTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACATGG
CCCGAGAGCT
GCATCCGGAC
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGCA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
C