名称: | pLV-EF1a-CA3(human)-IRES-BSD |
---|---|
别称: | NM_005181.4;CAIII; Car3 |
基因长: | 780bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV-EF1a-MCS-IRES-BSD |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AAGCTTAATG
TAGTCTTATG
CAATACTCTT
GTAGTCTTGC
AACATGGTAA
CGATGAGTTA
GCAACATGCC
TTACAAGGAG
AGAAAAAGCA
CCGTGCATGC
CGATTGGTGG
AAGTAAGGTG
GTACGATCGT
GCCTTATTAG
GAAGGCAACA
GACGGGTCTG
ACATGGATTG
GACGAACCAC
TGAATTGCCG
CATTGCAGAG
ATATTGTATT
TAAGTGCCTA
GCTCGATACA
TAAACGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTGG
CGCCCGAACA
GGGACTTGAA
AGCGAAAGGG
AAACCAGAGG
AGCTCTCTCG
ACGCAGGACT
CGGCTTGCTG
AAGCGCGCAC
GGCAAGAGGC
GAGGGGCGGC
GACTGGTGAG
TACGCCAAAA
ATTTTGACTA
GCGGAGGCTA
GAAGGAGAGA
GATGGGTGCG
AGAGCGTCAG
TATTAAGCGG
GGGAGAATTA
GATCGCGATG
GGAAAAAATT
CGGTTAAGGC
CAGGGGGAAA
GAAAAAATAT
AAATTAAAAC
ATATAGTATG
GGCAAGCAGG
GAGCTAGAAC
GATTCGCAGT
TAATCCTGGC
CTGTTAGAAA
CATCAGAAGG
CTGTAGACAA
ATACTGGGAC
AGCTACAACC
ATCCCTTCAG
ACAGGATCAG
AAGAACTTAG
ATCATTATAT
AATACAGTAG
CAACCCTCTA
TTGTGTGCAT
CAAAGGATAG
AGATAAAAGA
CACCAAGGAA
GCTTTAGACA
AGATAGAGGA
AGAGCAAAAC
AAAAGTAAGA
CCACCGCACA
GCAAGCGGCC
GCTGATCTTC
AGACCTGGAG
GAGGAGATAT
GAGGGACAAT
TGGAGAAGTG
AATTATATAA
ATATAAAGTA
GTAAAAATTG
AACCATTAGG
AGTAGCACCC
ACCAAGGCAA
AGAGAAGAGT
GGTGCAGAGA
GAAAAAAGAG
CAGTGGGAAT
AGGAGCTTTG
TTCCTTGGGT
TCTTGGGAGC
AGCAGGAAGC
ACTATGGGCG
CAGCGTCAAT
GACGCTGACG
GTACAGGCCA
GACAATTATT
GTCTGGTATA
GTGCAGCAGC
AGAACAATTT
GCTGAGGGCT
ATTGAGGCGC
AACAGCATCT
GTTGCAACTC
ACAGTCTGGG
GCATCAAGCA
GCTCCAGGCA
AGAATCCTGG
CTGTGGAAAG
ATACCTAAAG
GATCAACAGC
TCCTGGGGAT
TTGGGGTTGC
TCTGGAAAAC
TCATTTGCAC
CACTGCTGTG
CCTTGGAATG
CTAGTTGGAG
TAATAAATCT
CTGGAACAGA
TTTGGAATCA
CACGACCTGG
ATGGAGTGGG
ACAGAGAAAT
TAACAATTAC
ACAAGCTTAA
TACACTCCTT
AATTGAAGAA
TCGCAAAACC
AGCAAGAAAA
GAATGAACAA
GAATTATTGG
AATTAGATAA
ATGGGCAAGT
TTGTGGAATT
GGTTTAACAT
AACAAATTGG
CTGTGGTATA
TAAAATTATT
CATAATGATA
GTAGGAGGCT
TGGTAGGTTT
AAGAATAGTT
TTTGCTGTAC
TTTCTATAGT
GAATAGAGTT
AGGCAGGGAT
ATTCACCATT
ATCGTTTCAG
ACCCACCTCC
CAACCCCGAG
GGGACCCGAC
AGGCCCGAAG
GAATAGAAGA
AGAAGGTGGA
GAGAGAGACA
GAGACAGATC
CATTCGATTA
GTGAACGGAT
CTCGACGGTA
TCGGTTAACT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAATTCAA
AATTTTATCG
ATGCCTCCCC
GTCACCACCC
CCCCCAACCC
GCCCCGACCG
GAGCTGAGAG
TAATTCATAC
AAAAGGACTC
GCCCCTGCCT
TGGGGAATCC
CAGGGACCGT
CGTTAAACTC
CCACTAACGT
AGAACCCAGA
GATCGCTGCG
TTCCCGCCCC
CTCACCCGCC
CGCTCTCGTC
ATCACTGAGG
TGGAGAAGAG
CATGCGTGAG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GTAAGTGCCG
TGTGTGGTTC
CCGCGGGCCT
GGCCTCTTTA
CGGGTTATGG
CCCTTGCGTG
CCTTGAATTA
CTTCCACGCC
CCTGGCTGCA
GTACGTGATT
CTTGATCCCG
AGCTTCGGGT
TGGAAGTGGG
TGGGAGAGTT
CGAGGCCTTG
CGCTTAAGGA
GCCCCTTCGC
CTCGTGCTTG
AGTTGAGGCC
TGGCCTGGGC
GCTGGGGCCG
CCGCGTGCGA
ATCTGGTGGC
ACCTTCGCGC
CTGTCTCGCT
GCTTTCGATA
AGTCTCTAGC
CATTTAAAAT
TTTTGATGAT
ATCCTGCGAC
GCTTTTTTTC
TGGCAAGATA
GTCTTGTAAA
TGCGGGCCAA
GATCTGCACA
CTGGTATTTC
GGTTTTTGGG
GCCGCGGGCG
GCGACGGGGC
CCGTGCGTCC
CAGCGCACAT
GTTCGGCGAG
GCGGGGCCTG
CGAGCGCGGC
CACCGAGAAT
CGGACGGGGG
TAGTCTCAAG
CTGGCCGGCC
TGCTCTGGTG
CCTGGCCTCG
CGCCGCCGTG
TATCGCCCCG
CCCTGGGCGG
CAAGGCTGGC
CCGGTCGGCA
CCAGTTGCGT
GAGCGGAAAG
ATGGCCGCTT
CCCGGCCCTG
CTGCAGGGAG
CTCAAAATGG
AGGACGCGGC
GCTCGGGAGA
GCGGGCGGGT
GAGTCACCCA
CACAAAGGAA
AAGGGCCTTT
CCGTCCTCAG
CCGTCGCTTC
ATGTGACTCC
ACGGAGTACC
GGGCGCCGTC
CAGGCACCTC
GATTAGTTCT
CGAGCTTTTG
GAGTACGTCG
TCTTTAGGTT
GGGGGGAGGG
GTTTTATGCG
ATGGAGTTTC
CCCACACTGA
GTGGGTGGAG
ACTGAAGTTA
GGCCAGCTTG
GCACTTGATG
TAATTCTCCT
TGGAATTTGC
CCTTTTTGAG
TTTGGATCTT
GGTTCATTCT
CAAGCCTCAG
ACAGTGGTTC
AAAGTTTTTT
TCTTCCATTT
CAGGTGTCGT
GAAAACTACC
CCTCTAGAGC
CACCATGGCC
AAGGAGTGGG
GCTACGCCAG
TCACAACGGT
CCTGACCACT
GGCATGAACT
TTTCCCAAAT
GCCAAGGGGG
AAAACCAGTC
GCCCGTTGAG
CTGCATACTA
AAGACATCAG
GCATGACCCT
TCTCTGCAGC
CATGGTCTGT
GTCTTATGAT
GGTGGCTCTG
CCAAGACCAT
CCTGAATAAT
GGGAAGACCT
GCCGAGTTGT
ATTTGATGAT
ACTTATGATA
GGTCAATGCT
GAGAGGGGGT
CCTCTCCCTG
GACCCTACCG
ACTTCGCCAG
TTTCATCTTC
ACTGGGGCTC
TTCGGATGAT
CATGGCTCTG
AGCACACCGT
GGATGGAGTC
AAGTATGCAG
CGGAGCTTCA
TTTGGTTCAC
TGGAACCCGA
AGTATAACAC
TTTTAAAGAA
GCCCTGAAGC
AGCGCGATGG
GATCGCTGTG
ATTGGCATTT
TTCTGAAGAT
AGGACATGAG
AATGGCGAGT
TCCAGATTTT
CCTTGATGCA
TTGGACAAGA
TTAAGACAAA
GGGCAAGGAG
GCGCCCTTCA
CAAAGTTTGA
CCCATCCTGC
CTGTTCCCGG
CATGCCGGGA
CTACTGGACC
TACCAGGGCT
CATTCACCAC
GCCGCCCTGC
GAGGAATGCA
TTGTGTGGCT
GCTGCTGAAG
GAGCCCATGA
CCGTGAGCTC
TGACCAGATG
GCCAAGCTGC
GGAGCCTCCT
CTCCAGTGCT
GAGAACGAGC
CCCCAGTGCC
TCTTGTGAGC
AACTGGCGAC
CTCCACAGCC
TATCAATAAC
AGGGTGGTGA
GAGCTTCCTT
CAAATGAGGA
TCCACGCGTC
TCCGGCCTAG
GGATAACAGG
GTAATCCGCT
AGCCCCTCTC
CCTCCCCCCC
CCCTAACGTT
ACTGGCCGAA
GCCGCTTGGA
ATAAGGCCGG
TGTGCGTTTG
TCTATATGTT
ATTTTCCACC
ATATTGCCGT
CTTTTGGCAA
TGTGAGGGCC
CGGAAACCTG
GCCCTGTCTT
CTTGACGAGC
ATTCCTAGGG
GTCTTTCCCC
TCTCGCCAAA
GGAATGCAAG
GTCTGTTGAA
TGTCGTGAAG
GAAGCAGTTC
CTCTGGAAGC
TTCTTGAAGA
CAAACAACGT
CTGTAGCGAC
CCTTTGCAGG
CAGCGGAACC
CCCCACCTGG
CGACAGGTGC
CTCTGCGGCC
AAAAGCCACG
TGTATAAGAT
ACACCTGCAA
AGGCGGCACA
ACCCCAGTGC
CACGTTGTGA
GTTGGATAGT
TGTGGAAAGA
GTCAAATGGC
TCTCCTCAAG
CGTATTCAAC
AAGGGGCTGA
AGGATGCCCA
GAAGGTACCC
CATTGTATGG
GATCTGATCT
GGGGCCTCGG
TACACATGCT
TTACATGTGT
TTAGTCGAGG
TTAAAAAAAC
GTCTAGGCCC
CCCGAACCAC
GGGGACGTGG
TTTTCCTTTG
AAAAACACGA
TGATAATATG
GCCACACTAG
AGATCCACCG
GTCGCCACCA
TGGCCAAGCC
TTTGTCTCAA
GAAGAATCCA
CCCTCATTGA
AAGAGCAACG
GCTACAATCA
ACAGCATCCC
CATCTCTGAA
GACTACAGCG
TCGCCAGCGC
AGCTCTCTCT
AGCGACGGCC
GCATCTTCAC
TGGTGTCAAT
GTATATCATT
TTACTGGGGG
ACCTTGTGCA
GAACTCGTGG
TGCTGGGCAC
TGCTGCTGCT
GCGGCAGCTG
GCAACCTGAC
TTGTATCGTC
GCGATCGGAA
ATGAGAACAG
GGGCATCTTG
AGCCCCTGCG
GACGGTGCCG
ACAGGTGCTT
CTCGATCTGC
ATCCTGGGAT
CAAAGCCATA
GTGAAGGACA
GTGATGGACA
GCCGACGGCA
GTTGGGATTC
GTGAATTGCT
GCCCTCTGGT
TATGTGTGGG
AGGGCTAAGC
GGCCGCGTCG
ACAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAGCT
GACGTCCTTT
CCATGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CCTGGAATTC
GAGCTCGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATCTGCTTTT
TGCTTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTAGTAGTT
CATGTCATCT
TATTATTCAG
TATTTATAAC
TTGCAAAGAA
ATGAATATCA
GAGAGTGAGA
GGAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGGCTCTAG
CTATCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCGGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGGACGTAC
CCAATTCGCC
CTATAGTGAG
TCGTATTACG
CGCGCTCACT
GGCCGTCGTT
TTACAACGTC
GTGACTGGGA
AAACCCTGGC
GTTACCCAAC
TTAATCGCCT
TGCAGCACAT
CCCCCTTTCG
CCAGCTGGCG
TAATAGCGAA
GAGGCCCGCA
CCGATCGCCC
TTCCCAACAG
TTGCGCAGCC
TGAATGGCGA
ATGGGACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCCTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TAGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGTCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGCCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTTTAACA
AAATATTAAC
GCTTACAATT
TAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ATGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTATTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTT
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGGCGCAGC
GGTCGGGCTG
AACGGGGGGT
TCGTGCACAC
AGCCCAGCTT
GGAGCGAACG
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCTATGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCTG
GCACGACAGG
TTTCCCGACT
GGAAAGCGGG
CAGTGAGCGC
AACGCAATTA
ATGTGAGTTA
GCTCACTCAT
TAGGCACCCC
AGGCTTTACA
CTTTATGCTT
CCGGCTCGTA
TGTTGTGTGG
AATTGTGAGC
GGATAACAAT
TTCACACAGG
AAACAGCTAT
GACCATGATT
ACGCCAAGCG
CGCAATTAAC
CCTCACTAAA
GGGAACAAAA
GCTGGAGCTG
C