名称: | pMSGV1-ITGA8(human) |
---|---|
别称: | NM_003638.3 |
基因长: | 3192bp |
质粒宿主: | 哺乳细胞,逆病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pMSGV1 |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AATGAAAGAC
CCCACCTGTA
GGTTTGGCAA
GCTAGCTTAA
GTAACGCCAT
TTTGCAAGGC
ATGGAAAATA
CATAACTGAG
AATAGAGAAG
TTCAGATCAA
GGTTAGGAAC
AGAGAGACAG
CAGAATATGG
GCCAAACAGG
ATATCTGTGG
TAAGCAGTTC
CTGCCCCGGC
TCAGGGCCAA
GAACAGATGG
TCCCCAGATG
CGGTCCCGCC
CTCAGCAGTT
TCTAGAGAAC
CATCAGATGT
TTCCAGGGTG
CCCCAAGGAC
CTGAAATGAC
CCTGTGCCTT
ATTTGAACTA
ACCAATCAGT
TCGCTTCTCG
CTTCTGTTCG
CGCGCTTCTG
CTCCCCGAGC
TCAATAAAAG
AGCCCACAAC
CCCTCACTCG
GCGCGCCAGT
CCTCCGATAG
ACTGCGTCGC
CCGGGTACCC
GTATTCCCAA
TAAAGCCTCT
TGCTGTTTGC
ATCCGAATCG
TGGACTCGCT
GATCCTTGGG
AGGGTCTCCT
CAGATTGATT
GACTGCCCAC
CTCGGGGGTC
TTTCATTTGG
AGGTTCCACC
GAGATTTGGA
GACCCCTGCC
CAGGGACCAC
CGACCCCCCC
GCCGGGAGGT
AAGCTGGCCA
GCGGTCGTTT
CGTGTCTGTC
TCTGTCTTTG
TGCGTGTTTG
TGCCGGCATC
TAATGTTTGC
GCCTGCGTCT
GTACTAGTTA
GCTAACTAGC
TCTGTATCTG
GCGGACCCGT
GGTGGAACTG
ACGAGTTCGG
AACACCCGGC
CGCAACCCTG
GGAGACGTCC
CAGGGACTTC
GGGGGCCGTT
TTTGTGGCCC
GACCTGAGTC
CTAAAATCCC
GATCGTTTAG
GACTCTTTGG
TGCACCCCCC
TTAGAGGAGG
GATATGTGGT
TCTGGTAGGA
GACGAGAACC
TAAAACAGTT
CCCGCCTCCG
TCTGAATTTT
TGCTTTCGGT
TTGGGACCGA
AGCCGCGCCG
CGCGTCTTGT
CTGCTGCAGC
ATCGTTCTGT
GTTGTCTCTG
TCTGACTGTG
TTTCTGTATT
TGTCTGAAAA
TATGGGCCCG
GGCTAGCCTG
TTACCACTCC
CTTAAGTTTG
ACCTTAGGTC
ACTGGAAAGA
TGTCGAGCGG
ATCGCTCACA
ACCAGTCGGT
AGATGTCAAG
AAGAGACGTT
GGGTTACCTT
CTGCTCTGCA
GAATGGCCAA
CCTTTAACGT
CGGATGGCCG
CGAGACGGCA
CCTTTAACCG
AGACCTCATC
ACCCAGGTTA
AGATCAAGGT
CTTTTCACCT
GGCCCGCATG
GACACCCAGA
CCAGGTCCCC
TACATCGTGA
CCTGGGAAGC
CTTGGCTTTT
GACCCCCCTC
CCTGGGTCAA
GCCCTTTGTA
CACCCTAAGC
CTCCGCCTCC
TCTTCCTCCA
TCCGCCCCGT
CTCTCCCCCT
TGAACCTCCT
CGTTCGACCC
CGCCTCGATC
CTCCCTTTAT
CCAGCCCTCA
CTCCTTCTCT
AGGCGCCCCC
ATATGGCCAT
ATGAGATCTT
ATATGGGGCA
CCCCCGCCCC
TTGTAAACTT
CCCTGACCCT
GACATGACAA
GAGTTACTAA
CAGCCCCTCT
CTCCAAGCTC
ACTTACAGGC
TCTCTACTTA
GTCCAGCACG
AAGTCTGGAG
ACCTCTGGCG
GCAGCCTACC
AAGAACAACT
GGACCGACCG
GTGGTACCTC
ACCCTTACCG
AGTCGGCGAC
ACAGTGTGGG
TCCGCCGACA
CCAGACTAAG
AACCTAGAAC
CTCGCTGGAA
AGGACCTTAC
ACAGTCCTGC
TGACCACCCC
CACCGCCCTC
AAAGTAGACG
GCATCGCAGC
TTGGATACAC
GCCGCCCACG
TGAAGGCTGC
CGACCCCGGG
GGTGGACCAT
CCTCTAGCCC
TCGAGAAGCT
TGCCACCATG
TCGCCCGGGG
CCAGCCGCGG
TCCCCGGGGA
AGCCAGGCGC
CGCTGATCGC
GCCCCTCTGC
TGCGCCGCGG
CCGCGCTGGG
GATGTTGCTG
TGGTCCCCCG
CCTGTCAGGC
GTTCAACCTG
GACGTGGAAA
AGCTCACAGT
GTACAGCGGC
CCCAAGGGCA
GCTACTTCGG
CTACGCCGTG
GACTTCCACA
TACCCGACGC
CCGCACAGCG
AGTGTCTTGG
TGGGGGCGCC
CAAAGCCAAC
ACCAGCCAGC
CCGATATCGT
GGAAGGGGGA
GCCGTCTATT
ACTGTCCTTG
GCCCGCGGAG
GGGTCTGCGC
AGTGCAGGCA
GATACCGTTT
GACACCACCA
ACAACAGAAA
GATCAGAGTT
AATGGAACCA
AAGAACCTAT
CGAGTTCAAA
TCCAATCAGT
GGTTTGGAGC
AACAGTGAAA
GCTCACAAAG
GAAAAGTTGT
GGCCTGTGCT
CCTTTATATC
ACTGGAGAAC
TCTTAAACCG
ACACCAGAAA
AGGACCCAGT
TGGCACCTGC
TATGTAGCAA
TTCAGAACTT
CAGCGCCTAT
GCCGAGTTCT
CTCCTTGCCG
GAACAGCAAT
GCTGATCCGG
AAGGCCAGGG
TTACTGCCAA
GCAGGATTTA
GTCTGGATTT
TTATAAGAAT
GGAGACCTTA
TTGTGGGAGG
ACCTGGGAGT
TTCTACTGGC
AAGGACAAGT
GATCACTGCC
AGTGTTGCAG
ATATCATTGC
AAATTACTCA
TTCAAGGATA
TCCTCAGGAA
ACTGGCAGGA
GAAAAGCAGA
CGGAAGTGGC
TCCAGCTTCC
TATGATGACA
GTTACCTTGG
ATACTCAGTT
GCTGCTGGGG
AGTTTACTGG
GGATTCTCAG
CAAGAATTGG
TTGCTGGAAT
TCCAAGAGGA
GCACAGAATT
TTGGATATGT
TTCCATCATT
AACTCTACGG
ATATGACGTT
TATTCAGAAT
TTCACGGGAG
AACAGATGGC
ATCTTATTTT
GGATATACCG
TTGTCGTATC
AGATGTTAAC
AGTGATGGAC
TGGATGATGT
CCTGGTTGGG
GCACCTCTCT
TTATGGAACG
TGAATTTGAG
AGCAACCCCA
GAGAAGTAGG
GCAAATCTAC
CTGTATTTGC
AAGTGAGCTC
TCTCCTCTTC
AGAGACCCCC
AGATCCTCAC
TGGCACCGAG
ACGTTTGGGA
GATTCGGTAG
TGCTATGGCA
CACTTAGGAG
ACCTGAACCA
AGATGGATAC
AATGACATTG
CCATCGGAGT
GCCTTTTGCA
GGCAAGGATC
AAAGAGGCAA
AGTGCTCATT
TATAATGGGA
ACAAAGATGG
CTTAAACACC
AAGCCTTCCC
AAGTTCTGCA
AGGAGTGTGG
GCCTCACATG
CTGTCCCTTC
CGGATTTGGC
TTTACTTTAA
GAGGAGATTC
AGACATAGAC
AAGAATGATT
ACCCAGATTT
GATTGTGGGT
GCATTTGGAA
CAGGAAAAGT
CGCTGTTTAC
AGAGCAAGAC
CGGTTGTGAC
TGTAGATGCC
CAGCTTCTGC
TGCACCCAAT
GATTATCAAT
CTTGAAAATA
AAACTTGCCA
GGTTCCAGAC
TCTATGACAT
CTGCTGCCTG
CTTTTCTTTA
AGAGTATGTG
CATCTGTCAC
AGGCCAGAGC
ATTGCAAACA
CAATAGTCTT
GATGGCAGAG
GTGCAATTAG
ATTCCCTGAA
ACAGAAAGGA
GCTATTAAAC
GGACGCTCTT
CCTTGATAAC
CATCAGGCTC
ATCGCGTCTT
CCCTCTTGTG
ATAAAAAGGC
AGAAATCCCA
CCAGTGCCAG
GATTTCATCG
TTTACCTTCG
AGATGAAACT
GAATTCCGAG
ATAAATTATC
TCCAATCAAC
ATTAGTTTGA
ATTACAGTTT
GGACGAATCC
ACCTTTAAAG
AAGGCCTGGA
AGTGAAACCA
ATATTGAACT
ACTACAGAGA
AAACATTGTT
AGTGAACAGG
CTCACATTCT
GGTGGACTGT
GGAGAAGACA
ATCTGTGTGT
TCCTGACTTG
AAGCTGTCGG
CTAGACCAGA
TAAGCATCAG
GTAATCATTG
GAGATGAAAA
TCACCTTATG
CTCATAATAA
ATGCAAGAAA
TGAAGGGGAA
GGAGCATATG
AAGCTGAACT
CTTTGTAATG
ATACCAGAAG
AGGCAGATTA
TGTTGGAATC
GAACGCAACA
ACAAGGGATT
TCGACCACTG
AGCTGTGAGT
ACAAGATGGA
AAATGTAACC
AGGATGGTGG
TGTGTGACCT
TGGGAACCCT
ATGGTGTCTG
GAACAAATTA
TTCCCTGGGC
CTCCGATTTG
CAGTTCCACG
TCTTGAGAAA
ACAAACATGA
GCATTAACTT
CGATCTCCAA
ATCAGAAGTT
CCAACAAGGA
CAATCCAGAC
AGCAATTTTG
TGAGCCTGCA
AATCAACATC
ACTGCTGTAG
CGCAGGTGGA
AATAAGAGGA
GTGTCACACC
CTCCGCAGAT
TGTTCTGCCC
ATTCATAACT
GGGAACCAGA
AGAGGAGCCC
CACAAAGAGG
AGGAGGTTGG
ACCATTGGTG
GAACATATTT
ATGAGCTGCA
CAATATTGGA
CCAAGTACCA
TCAGTGACAC
CATCCTGGAG
GTGGGCTGGC
CTTTCTCTGC
CCGGGATGAA
TTTCTTCTCT
ATATTTTCCA
TATTCAAACT
CTGGGACCTC
TGCAGTGCCA
ACCAAATCCT
AATATCAATC
CACAGGATAT
AAAGCCTGCT
GCCTCCCCAG
AGGACACCCC
TGAGCTCAGC
GCCTTTTTGC
GAAACTCTAC
TATTCCTCAT
CTTGTCAGGA
AGAGGGATGT
ACATGTGGTC
GAATTCCACA
GACAGAGCCC
TGCAAAAATA
CTGAATTGTA
CAAATATCGA
GTGTTTACAA
ATCTCCTGTG
CAGTGGGACG
ACTCGAAGGA
GGAGAAAGCG
CAGTCCTGAA
AGTCAGGTCA
CGATTATGGG
CCCACACCTT
CCTCCAGAGA
AAAAATGATC
CCTATGCTCT
TGCATCCCTG
GTGTCCTTTG
AAGTTAAGAA
GATGCCTTAT
ACAGATCAGC
CAGCAAAACT
CCCAGAAGGA
AGCATAGTAA
TTAAGACATC
AGTTATTTGG
GCAACTCCGA
ATGTTTCCTT
CTCAATCCCA
TTATGGGTAA
TAATACTAGC
AATACTTCTT
GGATTGTTGG
TTCTCGCCAT
TTTAACCTTA
GCTTTATGGA
AGTGTGGATT
CTTTGACAGA
GCCAGACCTC
CTCAGGAGGA
CATGACCGAC
AGGGAACAGC
TGACAAATGA
CAAGACCCCT
GAGGCATGAG
TCGACGGTAC
CGCGGGCCCG
GGATCCGATA
AAATAAAAGA
TTTTATTTAG
TCTCCAGAAA
AAGGGGGGAA
TGAAAGACCC
CACCTGTAGG
TTTGGCAAGC
TAGCTTAAGT
AACGCCATTT
TGCAAGGCAT
GGAAAATACA
TAACTGAGAA
TAGAGAAGTT
CAGATCAAGG
TTAGGAACAG
AGAGACAGCA
GAATATGGGC
CAAACAGGAT
ATCTGTGGTA
AGCAGTTCCT
GCCCCGGCTC
AGGGCCAAGA
ACAGATGGTC
CCCAGATGCG
GTCCCGCCCT
CAGCAGTTTC
TAGAGAACCA
TCAGATGTTT
CCAGGGTGCC
CCAAGGACCT
GAAATGACCC
TGTGCCTTAT
TTGAACTAAC
CAATCAGTTC
GCTTCTCGCT
TCTGTTCGCG
CGCTTCTGCT
CCCCGAGCTC
AATAAAAGAG
CCCACAACCC
CTCACTCGGC
GCGCCAGTCC
TCCGATAGAC
TGCGTCGCCC
GGGTACCCGT
GTATCCAATA
AACCCTCTTG
CAGTTGCATC
CGACTTGTGG
TCTCGCTGTT
CCTTGGGAGG
GTCTCCTCTG
AGTGATTGAC
TACCCGTCAG
CGGGGGTCTT
TCATGGGTAA
CAGTTTCTTG
AAGTTGGAGA
ACAACATTCT
GAGGGTAGGA
GTCGAATATT
AAGTAATCCT
GACTCAATTA
GCCACTGTTT
TGAATCCACA
TACTCCAATA
CTCCTGAAAT
CCATCGATGG
AGTTCATTAT
GGACAGCGCA
GAAAGAGCTG
GGGAGAATTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCCCTTTC
GTCTCGCGCG
TTTCGGTGAT
GACGGTGAAA
ACCTCTGACA
CATGCAGCTC
CCGGAGACGG
TCACAGCTTG
TCTGTAAGCG
GATGCCGGGA
GCAGACAAGC
CCGTCAGGGC
GCGTCAGCGG
GTGTTGGCGG
GTGTCGGGGC
TGGCTTAACT
ATGCGGCATC
AGAGCAGATT
GTACTGAGAG
TGCACCATAT
GCGGTGTGAA
ATACCGCACA
GATGCGTAAG
GAGAAAATAC
CGCATCAGGC
GCCATTCGCC
ATTCAGGCTG
CGCAACTGTT
GGGAAGGGCG
ATCGGTGCGG
GCCTCTTCGC
TATTACGCCA
GCTGGCGAAA
GGGGGATGTG
CTGCAAGGCG
ATTAAGTTGG
GTAACGCCAG
GGTTTTCCCA
GTCACGACGT
TGTAAAACGA
CGGCGCAAGG
AATGGTGCAT
GCAAGGAGAT
GGCGCCCAAC
AGTCCCCCGG
CCACGGGGCC
TGCCACCATA
CCCACGCCGA
AACAAGCGCT
CATGAGCCCG
AAGTGGCGAG
CCCGATCTTC
CCCATCGGTG
ATGTCGGCGA
TATAGGCGCC
AGCAACCGCA
CCTGTGGCGC
CGGTGATGCC
GGCCACGATG
CGTCCGGCGT
AGAGGCGATT
AGTCCAATTT
GTTAAAGACA
GGATATCAGT
GGTCCAGGCT
CTAGTTTTGA
CTCAACAATA
TCACCAGCTG
AAGCCTATAG
AGTACGAGCC
ATAGATAAAA
TAAAAGATTT
TATTTAGTCT
CCAGAAAAAG
GGGGG