名称: | pNEAT1(human)-Fluc-SV40-hRluc-Neo |
---|---|
别称: | - |
基因长: | 2001bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 启动子检测 |
片段类型: | Promoter |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | Fluc,Rluc |
启动子: | NEAT1 |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pMCS-Fluc-SV40-hRluc-Neo |
正向引物: | cer region-F1 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATGCAAGCT
GATCCGGCTG
CTAACAAAGC
CCGAAAGGAA
GCTGAGTTGG
CTGCTGCCAC
CGCTGAGCAA
TAACTAGCAT
AACCCCTTGG
GGCGGCCGCT
TCGAGCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTAACA
ACAACAATTG
CATTCATTTT
ATGTTTCAGG
TTCAGGGGGA
GATGTGGGAG
GTTTTTTTAA
GCAAGTAAAA
CCTCTACAAA
TGTGGTAAAA
TCGAATTTTA
ACAAAATATT
AACGCTTACA
ATTTCCTGAT
GCGGTATTTT
CTCCTTACGC
ATCTGTGCGG
TATTTCACAC
CGCATACGCG
GATCTGCGCA
GCACCATGGC
CTGAAATAAC
CTCTGAAAGA
GGAACTTGGT
TAGGTACCTT
CTGAGGCGGA
AAGAACCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTTGATTCT
TCTGACACAA
CAGTCTCGAA
CCAAAGGCTG
GAGCCACCAT
GGCTTCCAAG
GTGTACGACC
CCGAGCAACG
CAAACGCATG
ATCACTGGGC
CTCAGTGGTG
GGCTCGCTGC
AAGCAAATGA
ACGTGCTGGA
CTCCTTCATC
AACTACTATG
ATTCCGAGAA
GCACGCCGAG
AACGCCGTGA
TTTTTCTGCA
TGGTAACGCT
GCCTCCAGCT
ACCTGTGGAG
GCACGTCGTG
CCTCACATCG
AGCCCGTGGC
TAGATGCATC
ATCCCTGATC
TGATCGGAAT
GGGTAAGTCC
GGCAAGAGCG
GGAATGGCTC
ATATCGCCTC
CTGGATCACT
ACAAGTACCT
CACCGCTTGG
TTCGAGCTGC
TGAACCTTCC
AAAGAAAATC
ATCTTTGTGG
GCCACGACTG
GGGGGCTTGT
CTGGCCTTTC
ACTACTCCTA
CGAGCACCAA
GACAAGATCA
AGGCCATCGT
CCATGCTGAG
AGTGTCGTGG
ACGTGATCGA
GTCCTGGGAC
GAGTGGCCTG
ACATCGAGGA
GGATATCGCC
CTGATCAAGA
GCGAAGAGGG
CGAGAAAATG
GTGCTTGAGA
ATAACTTCTT
CGTCGAGACC
ATGCTCCCAA
GCAAGATCAT
GCGGAAACTG
GAGCCTGAGG
AGTTCGCTGC
CTACCTGGAG
CCATTCAAGG
AGAAGGGCGA
GGTTAGACGG
CCTACCCTCT
CCTGGCCTCG
CGAGATCCCT
CTCGTTAAGG
GAGGCAAGCC
CGACGTCGTC
CAGATTGTCC
GCAACTACAA
CGCCTACCTT
CGGGCCAGCG
ACGATCTGCC
TAAGATGTTC
ATCGAGTCCG
ACCCTGGGTT
CTTTTCCAAC
GCTATTGTCG
AGGGAGCTAA
GAAGTTCCCT
AACACCGAGT
TCGTGAAGGT
GAAGGGCCTC
CACTTCAGCC
AGGAGGACGC
TCCAGATGAA
ATGGGTAAGT
ACATCAAGAG
CTTCGTGGAG
CGCGTGCTGA
AGAACGAGCA
GACCGGTGGT
GGGAGCGGAG
GTGGCGGATC
AGGTGGCGGA
GGCTCCGGAG
GGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AGGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGCGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
TGGCCGCAAT
AAAATATCTT
TATTTTCATT
ACATCTGTGT
GTTGGTTTTT
TGTGTGAATC
GATAGCGATA
ATCTTTGCGC
TTGCGTTTTC
CCTTGTCCAG
ATAGCCCAGT
AGCTGACATT
CATCCGGGGT
CAGCACCGTT
TCTGCGGACT
GGCTTTCTAC
GTAATGGTTT
CTTAGACGTC
AGGTGGCACT
TTTCGGGGAA
ATGTGCGCGG
AACCCCTATT
TGTTTATTTT
TCTAAATACA
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTC
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACTATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGGTCT
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ATTCGAAATG
ACCGACCAAG
CGACGCCCAA
CCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGG
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATTTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTATA
GTCCGGAAAT
ACAGGAACGC
ACGCTGGATG
GCCCTTCGCT
GGGATGGTGA
AACCATGAAA
AATGGCAGCT
TCAGTGGATT
AAGTGGGGGT
AATGTGGCCT
GTACCCTCTG
GTTGCATAGG
TATTCATACG
GTTAAAATTT
ATCAGGCGCG
ATTGCGGCAG
TTTTTCGGGT
GGTTTGTTGC
CATTTTTACC
TGTCTGCTGC
CGTGATCGCG
CTGAACGCGT
TTTAGCGGTG
CGTACAATTA
AGGGATTATG
GTAAATCCAC
TTACTGTCTG
CCCTCGTAGC
CATCGAGATA
AACCGCAGTA
CTCCGGCCAC
GATGCGTCCG
GCGTAGAGGA
TCGAGATCTC
GAGCTCAAGC
TTCGAATTCT
CTTGCTGTGT
TTCCCAGGCT
GGTCTCGAAC
TCCTGGCCTC
TAGGCAATCC
TCCCACCATG
GCCTTCAAAA
GCGAATGGAT
CCCACTCTTT
TTTGAAAAAT
AAAATCTACC
ATAGTTTGTT
TCTGGGATTT
GGGAATGATG
TGTGTGAATT
AGAGAGATTT
CGAGACCTGA
AATAGAGAAA
AGCAAGTTAA
GACATCAACT
GGGGCCCAGA
AACAGCACTA
CAGCGGGGAC
GACCCACCTC
TCAGATGCCT
GGTGAGATAA
AGAAAGGTCT
GGCTGACTCC
ACTGCCCAGT
ACTGTGAAGC
AGCAGTGAAG
ACTCCTCCAT
TGGTTTCATA
AGCTGCCCAG
CTTTCTAGAA
CATTTTGACA
GACAATTCCC
TCTTTCCACA
CGGTTCTTTC
AGGCCAAATC
ACCCCACCCC
AACCCACCCC
TGGAGATACA
GTCAGGAAGA
GACTGATGGG
GCAGATCCTG
GAGGAGGCCG
CACTGCCGGA
ATCTTCCCCT
GGCAGAGAAA
CAGGGCTAAG
CAGGCGCCAG
GGAGCAAGCC
TGGGCTTGCC
ACATCACCAC
CTTCTGTGCT
GAAGGTCAGA
TGACACACAG
TCACCAGTTT
TCCGAGAAGC
ACAGGGAGGG
GTGCATGTCA
GGCCCAGCCC
TGCCTGCTGA
TACCACCTCA
CAGACGCAGG
CGAAATGTCT
TCACCAGCTT
CTTCTCGGTT
GCCAAGGGGG
CCTGCTTTCA
AGGGGTTGTT
TTGTATGGGG
TGTGGGTGTT
AACCAGGGAG
AGGTTCCTGG
CAGGAGTTCC
TGTCAGATGC
CATTTTCCAT
TCTGGTTACT
TGAACCGGGG
TCCTTTTTCA
TCCTCTACCT
CAGGGGCCAG
AGCTTCCTCA
TCACCCTGCA
CCCCCTAGCA
AGGGAGGTGC
CCGCTACACG
GTCCAAAGTC
CTCTCCAGAC
ATTCGGGCCC
TGCCCGCCTT
GCTGGAGGGC
TCAGGAGTTC
ACCAGGTTTG
CTTCCAGAAG
AGCCAGGCTG
GGGATGCCTG
CTTGCAAATG
CAGCAAATAA
AAAATAAATA
AATAAATAAA
AATCACCTTT
CTCCCCACCC
CCACCCAAAT
GTAAAATGAG
CCGACAGCCT
GTCCCTCGGC
TATGTCAGAT
ACTGCTTTCC
GCACAATATC
TTGGTTTTAC
ATTATTTTGC
AACGGCCTCT
TCCCACTTAA
TCCATCCTGA
ACACACTTCT
TGAATGTTCT
ACCCCGGCAG
GGATGCGCAT
TCTCAGGAAA
CACGTCCCCT
CGCCAGGCCC
CTGGGAAGAG
TCAAAACCAC
GATGCCTGCC
CCTGAAGCGC
CCGCGCGGCT
CCACGGGGCT
CCATGTTGTC
ACCCACTAGC
TCCTGGACGC
TATCAGCCCG
CAGCAGGGTT
TCCTGGCCAG
AGAAACCGCC
TGTTGGGGTG
CGGAGCGTTC
CACGTCCCCT
CGCAGCACCC
CGCTGGCCTT
CTGGGCCGCC
CCGCCCCCAA
CCCGCGCCCG
GGCGCTGACG
TCATCGGCCG
AGCCCGACTC
GGAACCACCG
CCCGAAAGTC
ACGCGCGCCT
CCCGGCGGGA
CGTGCCCTCT
GCAGAGATCC
CTCCGCCGCC
GCCTGGAATT
TTCCAGATGT
CCTGCCGGCC
CTTCTTTCGG
GCCTCGGCTG
GGCGTGGTGA
CCTCAAGCGA
CCCCGGCGGC
TCCCGTCGCC
CACTCAAGAG
GGCGCCCCCG
CCCGCCACGC
CCCCAGCCCC
TCTCGGGGAA
GCGCGTCCCC
CGCCCGACCT
CAACAACATC
CGGGAAGAAA
AGGGGTCTTC
TTCCTCATGG
CATTCGCCTC
CCAAATGTCA
CCTTGAGCGC
TGTCCGCGAT
CCCAAAAAGC
ACTGTTAAAG
AGAAGCGGGG
ATACACTGGG
GTCCTTGCGT
GGGGGCCGCC
TGGGAGACCA
TGCACCGCCC
GGGAGTCTCT
CCGGGCAGGG
TCGGGGAGGG
ACTTTTTTGC
CGGTGGCCGT
GGAGGAATCG
TCCCGTTGAG
CAATGACCCC
GGTGACGCGG
CTGAGGGCTA
TAAAAGCAAA
AGTTGTGGCA
AGTCCAGCCG
GGATCCCACC
ATGGAAGATG
CCAAAAACAT
TAAGAAGGGC
CCAGCGCCAT
TCTACCCACT
CGAAGACGGG
ACCGCCGGCG
AGCAGCTGCA
CAAAGCCATG
AAGCGCTACG
CCCTGGTGCC
CGGCACCATC
GCCTTTACCG
ACGCACATAT
CGAGGTGGAC
ATTACCTACG
CCGAGTACTT
CGAGATGAGC
GTTCGGCTGG
CAGAAGCTAT
GAAGCGCTAT
GGGCTGAATA
CAAACCATCG
GATCGTGGTG
TGCAGCGAGA
ATAGCTTGCA
GTTCTTCATG
CCCGTGTTGG
GTGCCCTGTT
CATCGGTGTG
GCTGTGGCCC
CAGCTAACGA
CATCTACAAC
GAGCGCGAGC
TGCTGAACAG
CATGGGCATC
AGCCAGCCCA
CCGTCGTATT
CGTGAGCAAG
AAAGGGCTGC
AAAAGATCCT
CAACGTGCAA
AAGAAGCTAC
CGATCATACA
AAAGATCATC
ATCATGGATA
GCAAGACCGA
CTACCAGGGC
TTCCAAAGCA
TGTACACCTT
CGTGACTTCC
CATTTGCCAC
CCGGCTTCAA
CGAGTACGAC
TTCGTGCCCG
AGAGCTTCGA
CCGGGACAAA
ACCATCGCCC
TGATCATGAA
CAGTAGTGGC
AGTACCGGAT
TGCCCAAGGG
CGTAGCCCTA
CCGCACCGCA
CCGCTTGTGT
CCGATTCAGT
CATGCCCGCG
ACCCCATCTT
CGGCAACCAG
ATCATCCCCG
ACACCGCTAT
CCTCAGCGTG
GTGCCATTTC
ACCACGGCTT
CGGCATGTTC
ACCACGCTGG
GCTACTTGAT
CTGCGGCTTT
CGGGTCGTGC
TCATGTACCG
CTTCGAGGAG
GAGCTATTCT
TGCGCAGCTT
GCAAGACTAT
AAGATTCAAT
CTGCCCTGCT
GGTGCCCACA
CTATTTAGCT
TCTTCGCTAA
GAGCACTCTC
ATCGACAAGT
ACGACCTAAG
CAACTTGCAC
GAGATCGCCA
GCGGCGGGGC
GCCGCTCAGC
AAGGAGGTAG
GTGAGGCCGT
GGCCAAACGC
TTCCACCTAC
CAGGCATCCG
CCAGGGCTAC
GGCCTGACAG
AAACAACCAG
CGCCATTCTG
ATCACCCCCG
AAGGGGACGA
CAAGCCTGGC
GCAGTAGGCA
AGGTGGTGCC
CTTCTTCGAG
GCTAAGGTGG
TGGACTTGGA
CACCGGTAAG
ACACTGGGTG
TGAACCAGCG
CGGCGAGCTG
TGCGTCCGTG
GCCCCATGAT
CATGAGCGGC
TACGTTAACA
ACCCCGAGGC
TACAAACGCT
CTCATCGACA
AGGACGGCTG
GCTGCACAGC
GGCGACATCG
CCTACTGGGA
CGAGGACGAG
CACTTCTTCA
TCGTGGACCG
GCTGAAGAGC
CTGATCAAAT
ACAAGGGCTA
CCAGGTAGCC
CCAGCCGAAC
TGGAGAGCAT
CCTGCTGCAA
CACCCCAACA
TCTTCGACGC
CGGGGTCGCC
GGCCTGCCCG
ACGACGATGC
CGGCGAGCTG
CCCGCCGCAG
TCGTCGTGCT
GGAACACGGT
AAAACCATGA
CCGAGAAGGA
GATCGTGGAC
TATGTGGCCA
GCCAGGTTAC
AACCGCCAAG
AAGCTGCGCG
GTGGTGTTGT
GTTCGTGGAC
GAGGTGCCTA
AAGGACTGAC
CGGCAAGTTG
GACGCCCGCA
AGATCCGCGA
GATTCTCATT
AAGGCCAAGA
AGGGCGGCAA
GATCGCCGTG
TAATTCTAGT
TGTTTAAACG
AGCTCGCTAG
CCTCGAGTCT
AGAGTCGACC
TGCAGG