名称: | pM-Cas12a |
---|---|
别称: | Plasmid#196291 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | CaMV 35S |
复制子: | pUC |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCB301-2μ-HDV |
正向引物: | 35S |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AAGCTTGCAT
GCCTGCAGGT
CAACATGGTG
GAGCACGACA
CACTTGTCTA
CTCCAAAAAT
ATCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCAATTGAGA
CTTTTCAACA
AAGGGTAATA
TCCGGAAACC
TCCTCGGATT
CCATTGCCCA
GCTATCTGTC
ACTTTATTGT
GAAGATAGTG
GAAAAGGAAG
GTGGCTCCTA
CAAATGCCAT
CATTGCGATA
AAGGAAAGGC
CATCGTTGAA
GATGCCTCTG
CCGACAGTGG
TCCCAAAGAT
GGACCCCCAC
CCACGAGGAG
CATCGTGGAA
AAAGAAGACG
TTCCAACCAC
GTCTTCAAAG
CAAGTGGATT
GATGTGATAA
CATGGTGGAG
CACGACACAC
TTGTCTACTC
CAAAAATATC
AAAGATACAG
TCTCAGAAGA
CCAAAGGGCA
ATTGAGACTT
TTCAACAAAG
GGTAATATCC
GGAAACCTCC
TCGGATTCCA
TTGCCCAGCT
ATCTGTCACT
TTATTGTGAA
GATAGTGGAA
AAGGAAGGTG
GCTCCTACAA
ATGCCATCAT
TGCGATAAAG
GAAAGGCCAT
CGTTGAAGAT
GCCTCTGCCG
ACAGTGGTCC
CAAAGATGGA
CCCCCACCCA
CGAGGAGCAT
CGTGGAAAAA
GAAGACGTTC
CAACCACGTC
TTCAAAGCAA
GTGGATTGAT
GTGATATCTC
CACTGACGTA
AGGGATGACG
CACAATCCCA
CTATCCTTCG
CAAGACCCTT
CCTCTATATA
AGGAAGTTCA
TTTCATTTGG
AGAGGAGAGC
AATCAGTGCA
AACAAAAACC
TTAATCCAGA
CATCTTGAAA
TTAATCATAT
AACCATTATA
ATCTGAGCAG
ACGTATAAGA
TGGATTACAA
GGATGACGAC
GATAAGCCCG
GGGGATCCTC
TAGAGTCGAC
ATGGCTCCTA
AGAAGAAGCG
GAAGGTTGGT
ATTCACGGGG
TGCCTGCGGC
TTCAAAGCTC
GAGAAATTCA
CCAACTGTTA
TTCGTTGAGC
AAAACACTGC
GGTTTAAAGC
GATTCCAGTC
GGCAAGACTC
AAGAGAATAT
AGACAATAAG
CGGCTGTTGG
TGGAAGATGA
AAAGCGCGCG
GAAGACTACA
AAGGGGTGAA
GAAGTTGTTG
GACAGATACT
ACCTCTCTTT
TATCAATGAT
GTCTTGCACT
CAATCAAATT
GAAGAATCTG
AACAACTACA
TCTCCCTCTT
CAGAAAGAAA
ACAAGGACAG
AAAAGGAGAA
TAAGGAACTT
GAAAATTTGG
AGATCAATCT
GAGGAAAGAG
ATCGCGAAAG
CCTTTAAAGG
CAACGAAGGA
TACAAAAGTC
TGTTCAAGAA
GGATATAATT
GAGACAATTT
TGCCAGAGTT
CCTCGATGAC
AAGGACGAGA
TTGCGCTGGT
CAATTCGTTC
AACGGATTCA
CAACAGCATT
CACAGGCTTC
TTTGATAATC
GGGAAAATAT
GTTCTCTGAG
GAGGCAAAGT
CCACTTCTAT
TGCGTTCAGG
TGTATCAATG
AGAATCTCAC
TAGGTACATT
TCCAACATGG
ATATCTTTGA
GAATGTTGAC
GCAATTTTTG
ACAAGCACGA
AGTTCAGGAG
ATTAAGGAGA
AGATCCTCAA
TTCCGATTAT
GACGTTGAGG
ACTTCTTCGA
AGGTGAGTTT
TTTAATTTCG
TGCTCACTCA
AGAGGGTATC
GACGTGTATA
ATGCGATCAT
CGGTGGGTTC
GTGACTGAGT
CCGGTGAAAA
GATTAAGGGA
TTGAACGAGT
ATATCAACCT
TTACAACCAA
AAGACGAAAC
AGAAGCTGCC
AAAGTTCAAG
CCTCTTTACA
AACAGGTTCT
TTCAGACCGC
GAGTCACTCT
CGTTCTATGG
GGAGGGCTAC
ACTTCGGATG
AGGAAGTCCT
GGAGGTGTTC
AGGAATACTC
TCAATAAGAA
TTCGGAGATT
TTCTCTTCTA
TAAAAAAACT
GGAAAAGTTG
TTTAAGAATT
TTGACGAATA
CTCTAGCGCC
GGCATATTTG
TGAAAAACGG
CCCGGCCATA
TCAACGATAA
GTAAAGATAT
CTTCGGCGAA
TGGAACGTGA
TCAGAGACAA
ATGGAACGCG
GAGTATGACG
ATATTCACCT
GAAGAAGAAG
GCTGTCGTAA
CGGAGAAGTA
CGAGGATGAT
CGCAGGAAAA
GCTTCAAAAA
GATCGGAAGT
TTCAGCCTGG
AACAGTTGCA
GGAGTATGCT
GACGCCGATC
TTAGCGTCGT
CGAGAAGTTG
AAGGAGATAA
TCATCCAAAA
GGTCGACGAG
ATATATAAAG
TCTATGGATC
AAGTGAAAAA
CTGTTCGACG
CCGACTTCGT
TTTGGAGAAG
TCCCTGAAGA
AGAACGACGC
TGTTGTTGCC
ATTATGAAGG
ATCTGCTCGA
CAGCGTGAAG
AGTTTCGAGA
ACTATATTAA
GGCTTTTTTC
GGGGAGGGGA
AGGAGACTAA
CAGAGATGAG
TCCTTCTACG
GAGACTTCGT
CCTCGCGTAC
GATATACTCC
TTAAGGTAGA
CCACATCTAC
GACGCAATCA
GAAATTACGT
GACACAAAAG
CCGTACAGCA
AGGACAAGTT
CAAACTCTAC
TTCCAGAACC
CCCAGTTCAT
GGGCGGCTGG
GACAAGGACA
AGGAAACGGA
TTACAGGGCT
ACGATCCTGA
GGTATGGTTC
AAAATACTAC
TTGGCGATTA
TGGACAAGAA
GTACGCCAAG
TGTCTCCAGA
AGATTGACAA
AGACGATGTC
AATGGCAATT
ATGAGAAGAT
CAACTACAAG
CTGCTTCCGG
GTCCGAACAA
GATGCTCCCA
AAGGTTTTCT
TCAGCAAGAA
ATGGATGGCC
TACTATAACC
CAAGCGAGGA
CATCCAGAAG
ATTTATAAGA
ACGGTACGTT
CAAGAAGGGC
GACATGTTCA
ATCTTAACGA
CTGTCACAAG
CTGATCGACT
TCTTCAAAGA
CTCAATTAGC
CGGTACCCAA
AGTGGTCTAA
CGCCTATGAC
TTCAACTTTT
CGGAAACCGA
GAAGTACAAG
GATATAGCCG
GATTTTATAG
AGAGGTGGAA
GAGCAGGGCT
ACAAGGTGTC
ATTCGAGTCC
GCCAGCAAGA
AGGAAGTGGA
CAAGCTCGTG
GAAGATGGTA
AGCTCTACAT
GTTCCAGATT
TATAATAAAG
ACTTTAGCGA
TAAGAGCCAC
GGGACACCTA
ATCTCCACAC
AATGTATTTC
AAGCTGCTCT
TCGACGAGAA
TAACCACGGC
CAAATCAGGT
TGTCAGGAGG
GGCTGAACTC
TTCATGCGGC
GCGCTAGCCT
TAAGAAGGAG
GAGCTTGTAG
TCCACCCTGC
GAATAGTCCA
ATTGCGAATA
AGAACCCGGA
CAATCCTAAA
AAGACTACAA
CATTGAGCTA
CGACGTGTAC
AAGGATAAGA
GGTTTTCCGA
GGATCAGTAC
GAGCTCCACA
TCCCGATTGC
GATCAACAAG
TGCCCAAAGA
ATATTTTCAA
GATAAACACA
GAGGTGCGTG
TACTCCTGAA
GCATGACGAC
AATCCTTACG
TCATTGGGAT
TGATCGGGGC
GAGAGGAACC
TCCTCTATAT
TGTGGTGGTG
GACGGGAAGG
GGAACATAGT
CGAACAGTAC
TCCCTTAACG
AAATAATTAA
CAATTTCAAC
GGCATCCGTA
TCAAGACCGA
CTACCATTCG
TTGCTGGACA
AGAAGGAGAA
GGAGAGATTT
GAGGCGCGGC
AAAATTGGAC
AAGTATCGAG
AACATCAAGG
AACTCAAAGC
AGGTTATATC
TCTCAAGTTG
TGCATAAGAT
ATGCGAGCTG
GTTGAGAAGT
ATGACGCAGT
GATCGCTCTT
GAGGACCTCA
ACTCGGGCTT
TAAGAATTCT
AGAGTTAAAG
TGGAGAAGCA
GGTCTATCAA
AAGTTCGAGA
AGATGCTTAT
AGATAAGCTC
AACTACATGG
TCGATAAGAA
ATCGAACCCA
TGTGCCACCG
GCGGCGCACT
CAAAGGTTAC
CAAATAACAA
ACAAATTCGA
GTCCTTCAAA
TCGATTAGTA
CTCAGAATGG
GTTCATATTT
TATATACCGG
CGTGGCTTAC
GTCTAAGATC
GACCCGTCAA
CTGGTTTTGT
CAACCTGTTG
AAGACGAAAT
ACACGTCCAT
TGCCGATTCG
AAAAAGTTCA
TATCTAGTTT
TGATCGTATT
ATGTACGTCC
CAGAGGAAGA
TCTTTTCGAG
TTTGCTCTCG
ACTACAAAAA
CTTTTCGCGG
ACCGATGCGG
ATTACATTAA
AAAATGGAAA
CTCTATTCGT
ACGGCAACAG
AATCAGGATT
TTTCGCAACC
CTAAGAAGAA
TAACGTCTTT
GATTGGGAGG
AAGTTTGCTT
GACTAGCGCG
TACAAGGAGC
TCTTTAATAA
GTATGGCATT
AACTACCAAC
AGGGTGATAT
CAGAGCACTG
CTTTGCGAAC
AATCTGACAA
GGCTTTCTAC
TCATCCTTCA
TGGCTTTGAT
GAGCCTGATG
CTCCAGATGA
GAAATTCAAT
TACAGGCAGA
ACCGACGTGG
ATTTCTTGAT
CTCCCCGGTT
AAAAATTCTG
ATGGCATCTT
TTACGATAGC
AGGAACTATG
AAGCGCAAGA
GAATGCGATT
CTGCCAAAAA
ATGCAGACGC
CAACGGTGCC
TATAACATCG
CCAGGAAAGT
CCTGTGGGCG
ATCGGCCAGT
TCAAAAAGGC
CGAAGACGAA
AAATTGGACA
AGGTCAAAAT
CGCTATCAGC
AACAAAGAGT
GGCTGGAGTA
TGCTCAGACA
TCCGTAAAGC
ATAAGCGTCC
TGCTGCCACC
AAAAAGGCCG
GACAGGCTAA
GAAAAAGAAG
TGAGGCTGCT
TGTCACAAGC
TAAATTTGAT
CCTATTTCCT
ACATTAATCG
AATTATATAT
ATATAAAATA
AACAAAAAAC
AAACAAAAAT
ACAAATAAAC
AAAAAACAAC
AAAAAGAGTC
TTCGGACCAA
AGTTTGGCTT
TTCAGCCTTA
TTTTGTTTTT
GTTTTTTGTT
TTTGATTTTT
TGTTTGTTTT
TTATTTTTGT
TTTTGTTTGG
TTTTTGATTT
AAAAATGCAA
AATAAACAGA
GATTTAAGCT
TAAATAAGTG
GTATTAAAAG
TACTATATTT
CATCAAAAGA
TAACTGAGCA
ACATTATCAG
AAACCCCTTC
CTCCTCTTCT
TCAACTGATC
TCTCAAGATT
TGAGCTCAGT
TCTTTAAGCT
GTTTTTTAAT
CTGCTTCTCA
CTGTTTCCTT
TAGGAATTAT
CAGCTTGCAA
GCCTCAATGA
ATGCTTGAGA
TCTAGCTCTA
ATAGCCCTGT
TTAGAGGTAT
GACCATGCAA
CTTTTGTCTT
TATCAGCTCT
GGGTGAATCA
CAAAACTCCT
TTGTCCAAGA
ATACATGACA
CTACCAAAAG
AAACCCCCTT
CTTGTATTCT
TGACTGCACA
TCAAGTGCAG
CTGACAGCAG
TTTTCTGGAG
TGTTATTCAT
TTTCGGAATA
GACCAGTTCA
GATAAAAAAC
AAAACAGATA
GGATCAGTTA
TTGTCCCCTG
ACCCTTCAGA
ATGATTTGCT
TTTCAGATGG
CATGTTGGGA
TCGACCAGAG
CAACCACAAG
TTTCCCTGTA
GGGTTTGGTA
TAGTGGGGCA
GACCCATATC
ACAATCCTGG
AAATCATCAT
GTATTGTTTT
CTGCTGTCCC
AAGTTGGACA
GATCTTGATG
ACCTTATTAG
CGTTTTGCTT
CCCGTTGCCG
ACAAAAAGAT
CATTTTTCCA
GTTTGAGATA
TGGTGGTTTG
TATCTACTAT
CATTCTTGCA
GAAAGATCAT
AACCCTCTGA
TTCTGTGATA
GAATCAGATT
CATATGTTCC
AAAGGAAGAT
GTTCCCTCTG
TGTTCAACAG
TATCTTTCCT
TTGGATGTAT
CCATAGCTTT
GGTTAAAGCA
AGCTTTTCAT
CAGAAGAAGA
CCATGGTTTT
GAGACTTCAA
CATTACCGTT
ATGTTTAGCA
AGTGAAACTA
AAGGCCCTTC
ATCCTTTCTG
GCAGACTTAG
AAGACCCCTT
ATTACCAAAA
AAAGTCAACA
TTTTGAGTTC
AACAGCCTGA
GGTAGAGGAG
CTTGTGTAAT
GTAATTGATT
GATTCTTAGC
AAAGTGTATA
ATAGGTATAT
TTCTGATGCA
CTGATTGCTC
TGGGTCGGCA
TGGCATCTCC
ACCTCCTCGC
GGTCCGACCT
GGGCATCCGA
AGGAGGACGT
CGTCCACTCG
GATGGCTAAG
GGAGAGCTCG
AATTTCCCCG
ATCGTTCAAA
CATTTGGCAA
TAAAGTTTCT
TAAGATTGAA
TCCTGTTGCC
GGTCTTGCGA
TGATTATCAT
ATAATTTCTG
TTGAATTACG
TTAAGCATGT
AATAATTAAC
ATGTAATGCA
TGACGTTATT
TATGAGATGG
GTTTTTATGA
TTAGAGTCCC
GCAATTATAC
ATTTAATACG
CGATAGAAAA
CAAAATATAG
CGCGCAAACT
AGGATAAATT
ATCGCGCGCG
GTGTCATCTA
TGTTACTAGA
TCGGAATTCA
GATTGTCGTT
TCCCGCCTTC
AGTTTAAACT
ATCAGTGTTT
GACAGGATAT
ATTGGCGGGT
AAACCTAAGA
GAAAAGAGCG
TTTATTAGAA
TAATCGGATA
TTTAAAAGGG
CGTGAAAAGG
TTTATCCGTT
CGTCCATTTG
TATGTGCATG
CCAACCACAG
GAGATCTCAG
TAAAGCGAAA
AGTGCCACCT
GAACGAAGCA
TCTGTGCTTC
ATTTTGTAGA
ACAAAAATGC
AACGCGAGAG
CGCTAATTTT
TCAAACAAAG
AATCTGAGCT
GCATTTTTAC
AGAACAGAAA
TGCAACGCGA
AAGCGCTATT
TTACCAACGA
AGAATCTGTG
CTTCATTTTT
GTAAAACAAA
AATGCAACGC
GAGAGCGCTA
ATTTTTCAAA
CAAAGAATCT
GAGCTGCATT
TTTACAGAAC
AGAAATGCAA
CGCGAGAGCG
CTATTTTACC
AACAAAGAAT
CTATACTTCT
TTTTTGTTCT
ACAAAAATGC
ATCCCGAGAG
CGCTATTTTT
CTAACAAAGC
ATCTTAGATT
ACTTTTTTTC
TCCTTTGTGC
GCTCTATAAT
GCAGTCTCTT
GATAACTTTT
TGCACTGTAG
GTCCGTTAAG
GTTAGAAGAA
GGCTACTTTG
GTGTCTATTT
TCTCTTCCAT
AAAAAAAGCC
TGACTCCACT
TCCCGCGTTT
ACTGATTACT
AGCGAAGCTG
CGGGTGCATT
TTTTCAAGAT
AAAGGCATCC
CCGATTATAT
TCTATACCGA
TGTGGATTGC
GCATACTTTG
TGAACAGAAA
GTGATAGCGT
TGATGATTCT
TCATTGGTCA
GAAAATTATG
AACGGTTTCT
TCTATTTTGT
CTCTATATAC
TACGTATAGG
AAATGTTTAC
ATTTTCGTAT
TGTTTTCGAT
TCACTCTATG
AATAGTTCTT
ACTACAATTT
TTTTGTCTAA
AGAGTAATAC
TAGAGATAAA
CATAAAAAAT
GTAGAGGTCG
AGTTTAGATG
CAAGTTCAAG
GAGCGAAAGG
TGGATGGGTA
GGTTATATAG
GGATATAGCA
CAGAGATATA
TAGCAAAGAG
ATACTTTTGA
GCAATGTTTG
TGGAAGCGGT
ATTCGCAATA
TTTTAGTAGC
TCGTTACAGT
CCGGTGCGTT
TTTGGTTTTT
TGAAAGTGCG
TCTTCAGAGC
GCTTTTGGTT
TTCAAAAGCG
CTCTGAAGTT
CCTATACTTT
CTAGAGAATA
GGAACTTCGG
AATAGGAACT
TCAAAGCGTT
TCCGAAAACG
AGCGCTTCCG
AAAATGCAAC
GCGAGCTGCG
CACATACAGC
TCACTGTTCA
CGTCGCACCT
ATATCTGCGT
GTTGCCTGTA
TATATATATA
CATGAGAAGA
ACGGCATAGT
GCGTGTTTAT
GCTTAAATGC
GTACTTATAT
GCGTCTATTT
ATGTAGGATG
AAAGGTAGTC
TAGTACCTCC
TGTGATATTA
TCCCATTCCA
TGCGGGGTAT
CGTATGCTTC
CTTCAGCACT
ACCCTTTAGC
TGTTCTATAT
GCTGCCACTC
CTCAATTGGA
TTAGTCTCAT
CCTTCAATGC
TATCATTTCC
TTTGATATTG
GATCATACTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCTTTCG
TCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAGACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATAGA
TCAACGACAT
TACTATATAT
ATAATATAGG
AAGCATTTAA
TAGAACAGCA
TCGTAATATA
TGTGTACTTT
GCAGTTATGA
CGCCAGATGG
CAGTAGTGGA
AGATATTCTT
TATTGAAAAA
TAGCTTGTCA
CCTTACGTAC
AATCTTGATC
CGGAGCTTTT
CTTTTTTTGC
CGATTAAGAA
TTAATTCGGT
CGAAAAAAGA
AAAGGAGAGG
GCCAAGAGGG
AGGGCATTGG
TGACTATTGA
GCACGTGAGT
ATACGTGATT
AAGCACACAA
AGGCAGCTTG
GAGTATGTCT
GTTATTAATT
TCACAGGTAG
TTCTGGTCCA
TTGGTGAAAG
TTTGCGGCTT
GCAGAGCACA
GAGGCCGCAG
AATGTGCTCT
AGATTCCGAT
GCTGACTTGC
TGGGTATTAT
ATGTGTGCCC
AATAGAAAGA
GAACAATTGA
CCCGGTTATT
GCAAGGAAAA
TTTCAAGTCT
TGTAAAAGCA
TATAAAAATA
GTTCAGGCAC
TCCGAAATAC
TTGGTTGGCG
TGTTTCGTAA
TCAACCTAAG
GAGGATGTTT
TGGCTCTGGT
CAATGATTAC
GGCATTGATA
TCGTCCAACT
GCATGGAGAT
GAGTCGTGGC
AAGAATACCA
AGAGTTCCTC
GGTTTGCCAG
TTATTAAAAG
ACTCGTATTT
CCAAAAGACT
GCAACATACT
ACTCAGTGCA
GCTTCACAGA
AACCTCATTC
GTTTATTCCC
TTGTTTGATT
CAGAAGCAGG
TGGGACAGGT
GAACTTTTGG
ATTGGAACTC
GATTTCTGAC
TGGGTTGGAA
GGCAAGAGAG
CCCCGAAAGC
TTACATTTTA
TGTTAGCTGG
TGGACTGACG
CCAGAAAATG
TTGGTGATGC
GCTTAGATTA
AATGGCGTTA
TTGGTGTTGA
TGTAAGCGGA
GGTGTGGAGA
CAAATGGTGT
AAAAGACTCT
AACAAAATAG
CAAATTTCGT
CAAAAATGCT
AAGAAATAGG
TTATTACTGA
GTAGTATTTA
TTTAAGTATT
GTTTGTGCAC
TTGCCGATCT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GAAATTGTAA
GCGTTAATAT
TTTGTTAAAA
TTCGCGTTAA
ATTTTTGTTA
AATCAGCTCA
TTTTTTAACC
AATAGGCCGA
AATCGGCAAA
ATCCCTTATA
AATCAAAAGA
ATAGACCGAG
ATAGGGTTGA
GTGTTGTTCC
AGTTTGGAAC
AAGAGTCCAC
TATTAAAGAA
CGTGGACTCC
AACGTCAAAG
GGCGAAAAAC
CGTCTATCAG
GGCGATGGCC
CACTACGTGA
ACCATCACCC
TAATCAAGTT
TTTTGGGGTC
GAGGTGCCGT
AAAGCACTAA
ATCGGAACCC
TAAAGGGAGC
CCCCGATTTA
GAGCTTGACG
GGGAAAGCCG
GCGAACGTGG
CGAGAAAGGA
AGGGAAGAAA
GCGAAAGGAG
CGGGCGCTAG
GGCGCTGGCA
AGTGTAGCGG
TCACGCTGCG
CGTAACCACC
ACACCCGCCG
CGCTTAATGC
GCCGCTACAG
GGCGCGTCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGATC
CTTGCTGGCT
GAACCCCCAG
CCGGAACTGA
CCCCACAAGG
CCCTAGCGTT
TGCAATGCAC
CAGGTCATCA
TTGACCCAGG
CGTGTTCCAC
CAGGCCGCTG
CCTCGCAACT
CTTCGCAGGC
TTCGCCGACC
TGCTCGCGCC
ACTTCTTCAC
GCGGGTGGAA
TCCGATCCGC
ACATGAGGCG
GAAGGTTTCC
AGCTTGAGCG
GGTACGGCTC
CCGGTGCGAG
CTGAAATAGT
CGAACATCCG
TCGGGCCGTC
GGCGACAGCT
TGCGGTACTT
CTCCCATATG
AATTTCGTGT
AGTGGTCGCC
AGCAAACAGC
ACGACGATTT
CCTCGTCGAT
CAGGACCTGG
CAACGGGACG
TTTTCTTGCC
ACGGTCCAGG
ACGCGGAAGC
GGTGCAGCAG
CGACACCGAT
TCCAGGTGCC
CAACGCGGTC
GGACGTGAAG
CCCATCGCCG
TCGCCTGTAG
GCGCGACAGG
CATTCCTCGG
CCTTCGTGTA
ATACCGGCCA
TTGATCGACC
AGCCCAGGTC
CTGGCAAAGC
TCGTAGAACG
TGAAGGTGAT
CGGCTCGCCG
ATAGGGGTGC
GCTTCGCGTA
CTCCAACACC
TGCTGCCACA
CCAGTTCGTC
ATCGTCGGCC
CGCAGCTCGA
CGCCGGTGTA
GGTGATCTTC
ACGTCCTTGT
TGACGTGGAA
AATGACCTTG
TTTTGCAGCG
CCTCGCGCGG
GATTTTCTTG
TTGCGCGTGG
TGAACAGGGC
AGAGCGGGCC
GTGTCGTTTG
GCATCGCTCG
CATCGTGTCC
GGCCACGGCG
CAATATCGAA
CAAGGAAAGC
TGCATTTCCT
TGATCTGCTG
CTTCGTGTGT
TTCAGCAACG
CGGCCTGCTT
GGCCTCGCTG
ACCTGTTTTG
CCAGGTCCTC
GCCGGCGGTT
TTTCGCTTCT
TGGTCGTCAT
AGTTCCTCGC
GTGTCGATGG
TCATCGACTT
CGCCAAACCT
GCCGCCTCCT
GTTCGAGACG
ACGCGAACGC
TCCACGGCGG
CCGATGGCGC
GGGCAGGGCA
GGGGGAGCCA
GTTGCACGCT
GTCGCGCTCG
ATCTTGGCCG
TAGCTTGCTG
GGCCATCGAG
CCGACGGACT
GGAAGGTTTC
GCGGGGCGCA
CGCATGACGG
TGCGGCTTGC
GATGGTTTCG
GCATCCTCGG
CGGAAAACCC
CGCGTCGATC
AGTTCTTGCC
TGTATGCCTT
CCGGTCAAAC
GTCCGATTCA
TTCACCCTCC
TTGCGGGATT
GCCCCGACTC
ACGCCGGGGC
AATGTGCCCT
TATTCCTGAT
TTGACCCGCC
TGGTGCCTTG
GTGTCCAGAT
AATCCACCTT
ATCGGCAATG
AAGTCGGTCC
CGTAGACCGT
CTGGCCGTCC
TTCTCGTACT
TGGTATTCCG
AATCTTGCCC
TGCACGAATA
CCAGCGACCC
CTTGCCCAAA
TACTTGCCGT
GGGCCTCGGC
CTGAGAGCCA
AAACACTTGA
TGCGGAAGAA
GTCGGTGCGC
TCCTGCTTGT
CGCCGGCATC
GTTGCGCCAC
ATCTAGGTAC
TAAAACAATT
CATCCAGTAA
AATATAATAT
TTTATTTTCT
CCCAATCAGG
CTTGATCCCC
AGTAAGTCAA
AAAATAGCTC
GACATACTGT
TCTTCCCCGA
TATCCTCCCT
GATCGACCGG
ACGCAGAAGG
CAATGTCATA
CCACTTGTCC
GCCCTGCCGC
TTCTCCCAAG
ATCAATAAAG
CCACTTACTT
TGCCATCTTT
CACAAAGATG
TTGCTGTCTC
CCAGGTCGCC
GTGGGAAAAG
ACAAGTTCCT
CTTCGGGCTT
TTCCGTCTTT
AAAAAATCAT
ACAGCTCGCG
CGGATCTTTA
AATGGGGTGT
CTTCTTCCCA
GTTTTCGCAA
TCCACATCGG
CCAGATCGTT
ATTCAGTAAG
TAATCCAATT
CGGCTAAGCG
GCTGTCTAAG
CTATTCGTAT
AGGGACAATC
CGATATGTCG
ATGGAGTGAA
AGAGCCTGAT
GCACTCCGCA
TACAGCTCGA
TAATCTTTTC
AGGGCTTTGT
TCATCTTCAT
ACTCTTCCGA
GCAAAGGACG
CCATCGGCCT
CACTCATGAG
CAGATTGCTC
CAGCCATCAT
GCCGTTCAAG
GTGCAGGACC
TTTGGAACAG
GCAGCTTTCC
TTCCAGCCAT
AGCATCATGT
CCTTTTCCCG
TTCCACATCA
TAGGTGGTCC
CTTTATACCG
GCTGTCCGTC
ATTTTTAAAT
ATAGGTTTTC
ATTTTCTCCC
ACCAGCTTAT
ATACCTTAGC
AGGAGACATT
CCTTCCGTAT
CTTTTACGCA
GCGGTATTTT
TCGATCAGTT
TTTTCAATTC
CGGTGATATT
CTCATTTTAG
CCATTTATTA
TTTCCTTCCT
CTTTTCTACA
GTATTTAAAG
ATACCCCAAG
AAGCTAATTA
TAACAAGACG
AACTCCAATT
CACTGTTCCT
TGCATTCTAA
AACTTTAAAT
ACCAGAAAAC
AGCTTTTTCA
AAGTTGTTTT
CAAAGTTGGC
GTATAACATA
GTATCGACGG
AGCCGATTTT
GAAACCACAA
TTATGGGTGA
TGCTGCAACT
CGAGCGGGCC
GGGAGGGTTC
GAGAAGGGGG
GGCACCCCCC
TTCGGCGTGC
GCGGTCACGC
GCACAGGGCG
CAGCCCTGGT
TAAAAACAAG
GTTTATAAAT
ATTGGTTTAA
AAGCAGGTTA
AAAGACAGGT
TAGCGGTGGC
CGAAAAACGG
GCGGAAACCC
TTGCAAATGC
TGGATTTTCT
GCCTGTGGAC
AGCCCCTCAA
ATGTCAATAG
GTGCGCCCCT
CATCTGTCAG
CACTCTGCCC
CTCAAGTGTC
AAGGATCGCG
CCCCTCATCT
GTCAGTAGTC
GCGCCCCTCA
AGTGTCAATA
CCGCAGGGCA
CTTATCCCCA
GGCTTGTCCA
CATCATCTGT
GGGAAACTCG
CGTAAAATCA
GGCGTTTTCG
CCGATTTGCG
AGGCTGGCCA
GCTCCACGTC
GCCGGCCGAA
ATCGAGCCTG
CCCCTCATCT
GTCAACGCCG
CGCCGGGTGA
GTCGGCCCCT
CAAGTGTCAA
CGTCCGCCCC
TCATCTGTCA
GTGAGGGCCA
AGTTTTCCGC
GAGGTATCCA
CAACGCCGGC
GGCCGCGGTG
TCTCGCACAC
GGCTTCGACG
GCGTTTCTGG
CGCGTTTGCA
GGGCCATAGA
CGGCCGCCAG
CCCAGCGGCG
AGGGCAACCA
GCCCGGTGAG
CGTCTCTAGT
GGACTGATGG
GCTGCCTGTA
TCGAGTGGTG
ATTTTGTGCC
GAGCTGCCGG
TCGGGGAGCT
GTTGGCTGGC
TGGTGGCAGG
ATATATTGTG
GTGTAAACAA
ATTGACGCTT
AGACAACTTA
ATAACACATT
GCGGACGTTT
TTAATGTACT
GGGGTGGTTT
T