名称: | pLV3-CMV-GAL4-ELK1(human)-P2A-EGFP-KLB(human)-FLAG-Puro |
---|---|
别称: | |
基因长: | 1284+3132bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCATGAA
GCTACTGTCT
TCTATCGAAC
AAGCATGCGA
TATTTGCCGA
CTTAAAAAGC
TCAAGTGCTC
CAAAGAAAAA
CCGAAGTGCG
CCAAGTGTCT
GAAGAACAAC
TGGGAGTGTC
GCTACTCTCC
CAAAACCAAA
AGGTCTCCGC
TGACTAGGGC
ACATCTGACA
GAAGTGGAAT
CAAGGCTAGA
AAGACTGGAA
CAGCTATTTC
TACTGATTTT
TCCTCGAGAA
GACCTTGACA
TGATTTTGAA
AATGGATTCT
TTACAGGATA
TAAAAGCATT
GTTAACAGGA
TTATTTGTAC
AAGATAATGT
GAATAAAGAT
GCCGTCACAG
ATAGATTGGC
TTCAGTGGAG
ACTGATATGC
CTCTAACATT
GAGACAGCAT
AGAATAAGTG
CGACATCATC
ATCGGAAGAG
AGTAGTAACA
AAGGTCAAAG
ACAGTTGACT
GTATCGATGG
ACCCATCTGT
GACGCTGTGG
CAGTTTCTGC
TGCAGCTGCT
GAGAGAGCAA
GGCAATGGCC
ACATCATCTC
CTGGACTTCA
CGGGATGGTG
GTGAATTCAA
GCTGGTGGAT
GCAGAGGAGG
TGGCCCGGCT
GTGGGGGCTA
CGCAAGAACA
AGACCAACAT
GAATTACGAC
AAGCTCAGCC
GGGCCTTGCG
GTACTACTAT
GACAAGAACA
TCATCCGCAA
GGTGAGCGGC
CAGAAGTTCG
TCTACAAGTT
TGTGTCCTAC
CCTGAGGTCG
CAGGGTGCTC
CACTGAGGAC
TGCCCGCCCC
AGCCAGAGGT
GTCTGTTACC
TCCACCATGC
CAAATGTGGC
CCCTGCTGCT
ATACATGCCG
CCCCAGGGGA
CACTGTCTCT
GGAAAGCCAG
GCACACCCAA
GGGTGCAGGA
ATGGCAGGCC
CAGGCGGTTT
GGCACGCAGC
AGCCGGAACG
AGTACATGCG
CTCGGGCCTC
TATTCCACCT
TCACCATCCA
GTCTCTGCAG
CCGCAGCCAC
CCCCTCATCC
TCGGCCTGCT
GTGGTGCTCC
CCAGTGCAGC
TCCTGCAGGG
GCAGCAGCGC
CCCCCTCGGG
GAGCAGGAGC
ACCAGTCCAA
GCCCCTTGGA
GGCCTGTCTG
GAGGCTGAAG
AGGCCGGCTT
GCCTCTGCAG
GTCATCCTGA
CCCCGCCCGA
GGCCCCAAAC
CTGAAATCGG
AAGAGCTTAA
TGTGGAGCCG
GGTTTGGGCC
GGGCTTTGCC
CCCAGAAGTG
AAAGTAGAAG
GGCCCAAGGA
AGAGTTGGAA
GTTGCGGGGG
AGAGAGGGTT
TGTGCCAGAA
ACCACCAAGG
CCGAGCCAGA
AGTCCCTCCA
CAGGAGGGCG
TGCCAGCCCG
GCTGCCCGCG
GTTGTTATGG
ACACCGCAGG
GCAGGCGGGC
GGCCATGCGG
CTTCCAGCCC
TGAGATCTCC
CAGCCGCAGA
AGGGCCGGAA
GCCCCGGGAC
CTAGAGCTTC
CACTCAGCCC
GAGCCTGCTA
GGTGGGCCGG
GACCCGAACG
GACCCCAGGA
TCGGGAAGTG
GCTCCGGCCT
CCAGGCTCCG
GGGCCGGCGC
TGACCCCATC
CCTGCTTCCT
ACGCATACAT
TGACCCCGGT
GCTGCTGACA
CCCAGCTCGC
TGCCTCCTAG
CATTCACTTC
TGGAGCACCC
TGAGTCCCAT
TGCGCCCCGT
AGCCCGGCCA
AGCTCTCCTT
CCAGTTTCCA
TCCAGTGGCA
GCGCCCAGGT
GCACATCCCT
TCTATCAGCG
TGGATGGCCT
CTCGACCCCC
GTGGTGCTCT
CCCCAGGGCC
CCAGAAGCCA
GGATCCGGCG
CAACAAACTT
CTCTCTGCTG
AAACAAGCCG
GAGATGTCGA
AGAGAATCCT
GGACCGATGG
TGTCTAAGGG
CGAAGAGCTG
TTCACCGGGG
TGGTGCCCAT
CCTGGTCGAG
CTGGACGGCG
ACGTAAACGG
CCACAAGTTC
AGCGTGTCCG
GCGAGGGCGA
GGGCGATGCC
ACCTACGGCA
AGCTGACCCT
GAAGTTCATC
TGCACCACCG
GCAAGCTGCC
CGTGCCCTGG
CCCACCCTCG
TGACCACCCT
GACCTACGGC
GTGCAGTGCT
TCAGCCGCTA
CCCCGACCAC
ATGAAGCAGC
ACGACTTCTT
CAAGTCCGCC
ATGCCCGAAG
GCTACGTCCA
GGAGCGCACC
ATCTTCTTCA
AGGACGACGG
CAACTACAAG
ACCCGCGCCG
AGGTGAAGTT
CGAGGGCGAC
ACCCTGGTGA
ACCGCATCGA
GCTGAAGGGC
ATCGACTTCA
AGGAGGACGG
CAACATCCTG
GGGCACAAGC
TGGAGTACAA
CTACAACAGC
CACAACGTCT
ATATCATGGC
CGACAAGCAG
AAGAACGGCA
TCAAGGTGAA
CTTCAAGATC
CGCCACAACA
TCGAGGACGG
CAGCGTGCAG
CTCGCCGACC
ACTACCAGCA
GAACACCCCC
ATCGGCGACG
GCCCCGTGCT
GCTGCCCGAC
AACCACTACC
TGAGCACCCA
GTCCGCCCTG
AGCAAAGACC
CCAACGAGAA
GCGCGATCAC
ATGGTCCTGC
TGGAGTTCGT
GACCGCCGCC
GGGATCACTC
TCGGCATGGA
CGAGCTGTAC
AAGGGTGGAG
GCGGATCAGG
AGGCGGTGGA
TCCATGAAGC
CAGGCTGTGC
GGCAGGATCT
CCAGGGAATG
AATGGATTTT
CTTCAGCACT
GATGAAATAA
CCACACGCTA
TAGGAATACA
ATGTCCAACG
GGGGATTGCA
AAGATCTGTC
ATCCTGTCAG
CACTTATTCT
GCTACGAGCT
GTTACTGGAT
TCTCTGGAGA
TGGAAGAGCT
ATATGGTCTA
AAAATCCTAA
TTTTACTCCG
GTAAATGAAA
GTCAGCTGTT
TCTCTATGAC
ACTTTCCCTA
AAAACTTTTT
CTGGGGTATT
GGGACTGGAG
CATTGCAAGT
GGAAGGGAGT
TGGAAGAAGG
ATGGAAAAGG
ACCTTCTATA
TGGGATCATT
TCATCCACAC
ACACCTTAAA
AATGTCAGCA
GCACGAATGG
TTCCAGTGAC
AGTTATATTT
TTCTGGAAAA
AGACTTATCA
GCCCTGGATT
TTATAGGAGT
TTCTTTTTAT
CAATTTTCAA
TTTCCTGGCC
AAGGCTTTTC
CCCGATGGAA
TAGTAACAGT
TGCCAACGCA
AAAGGTCTGC
AGTACTACAG
TACTCTTCTG
GACGCTCTAG
TGCTTAGAAA
CATTGAACCT
ATAGTTACTT
TATACCACTG
GGATTTGCCT
TTGGCACTAC
AAGAAAAATA
TGGGGGGTGG
AAAAATGATA
CCATAATAGA
TATCTTCAAT
GACTATGCCA
CATACTGTTT
CCAGATGTTT
GGGGACCGTG
TCAAATATTG
GATTACAATT
CACAACCCAT
ATCTAGTGGC
TTGGCATGGG
TATGGGACAG
GTATGCATGC
CCCTGGAGAG
AAGGGAAATT
TAGCAGCTGT
CTACACTGTG
GGACACAACT
TGATCAAGGC
TCACTCGAAA
GTTTGGCATA
ACTACAACAC
ACATTTCCGC
CCACATCAGA
AGGGTTGGTT
ATCGATCACG
TTGGGATCTC
ATTGGATCGA
GCCAAACCGG
TCGGAAAACA
CGATGGATAT
ATTCAAATGT
CAACAATCCA
TGGTTTCTGT
GCTTGGATGG
TTTGCCAACC
CTATCCATGG
GGATGGCGAC
TATCCAGAGG
GGATGAGAAA
GAAGTTGTTC
TCCGTTCTAC
CCATTTTCTC
TGAAGCAGAG
AAGCATGAGA
TGAGAGGCAC
AGCTGATTTC
TTTGCCTTTT
CTTTTGGACC
CAACAACTTC
AAGCCCCTAA
ACACCATGGC
TAAAATGGGA
CAAAATGTTT
CACTTAATTT
AAGAGAAGCG
CTGAACTGGA
TTAAACTGGA
ATACAACAAC
CCTCGAATCT
TGATTGCTGA
GAATGGCTGG
TTCACAGACA
GTCGTGTGAA
AACAGAAGAC
ACCACGGCCA
TCTACATGAT
GAAGAATTTC
CTCAGCCAGG
TGCTTCAAGC
AATAAGGTTA
GATGAAATAC
GAGTGTTTGG
TTATACTGCC
TGGTCTCTCC
TGGATGGCTT
TGAATGGCAG
GATGCTTACA
CCATCCGCCG
AGGATTATTT
TATGTGGATT
TTAACAGTAA
ACAGAAAGAG
CGGAAACCTA
AGTCTTCAGC
ACACTACTAC
AAACAGATCA
TACGAGAAAA
TGGTTTTTCT
TTAAAAGAGT
CCACGCCAGA
TGTGCAGGGC
CAGTTTCCCT
GTGACTTCTC
CTGGGGTGTC
ACTGAATCTG
TTCTTAAGCC
CGAGTCTGTG
GCTTCGTCCC
CACAGTTCAG
CGATCCTCAT
CTGTACGTGT
GGAACGCCAC
TGGCAACAGA
CTGTTGCACC
GAGTGGAAGG
GGTGAGGCTG
AAAACACGAC
CCGCTCAATG
CACAGATTTT
GTAAACATCA
AAAAACAACT
TGAGATGTTG
GCAAGAATGA
AAGTCACCCA
CTACCGGTTT
GCTCTGGATT
GGGCCTCGGT
CCTTCCCACT
GGCAACCTGT
CCGCGGTGAA
CCGACAGGCC
CTGAGGTACT
ACAGGTGCGT
GGTCAGTGAG
GGGCTGAAGC
TTGGCATCTC
CGCGATGGTC
ACCCTGTATT
ATCCGACCCA
CGCCCACCTA
GGCCTCCCCG
AGCCTCTGTT
GCATGCCGAC
GGGTGGCTGA
ACCCATCGAC
GGCCGAGGCC
TTCCAGGCCT
ACGCTGGGCT
GTGCTTCCAG
GAGCTGGGGG
ACCTGGTGAA
GCTCTGGATC
ACCATCAACG
AGCCTAACCG
GCTAAGTGAC
ATCTACAACC
GCTCTGGCAA
CGACACCTAC
GGGGCGGCGC
ACAACCTGCT
GGTGGCCCAC
GCCCTGGCCT
GGCGCCTCTA
CGACCGGCAG
TTCAGGCCCT
CACAGCGCGG
GGCCGTGTCG
CTGTCGCTGC
ACGCGGACTG
GGCGGAACCC
GCCAACCCCT
ATGCTGACTC
GCACTGGAGG
GCGGCCGAGC
GCTTCCTGCA
GTTCGAGATC
GCCTGGTTCG
CCGAGCCGCT
CTTCAAGACC
GGGGACTACC
CCGCGGCCAT
GAGGGAATAC
ATTGCCTCCA
AGCACCGACG
GGGGCTTTCC
AGCTCGGCCC
TGCCGCGCCT
CACCGAGGCC
GAAAGGAGGC
TGCTCAAGGG
CACGGTCGAC
TTCTGCGCGC
TCAACCACTT
CACCACTAGG
TTCGTGATGC
ACGAGCAGCT
GGCCGGCAGC
CGCTACGACT
CGGACAGGGA
CATCCAGTTT
CTGCAGGACA
TCACCCGCCT
GAGCTCCCCC
ACGCGCCTGG
CTGTGATTCC
CTGGGGGGTG
CGCAAGCTGC
TGCGGTGGGT
CCGGAGGAAC
TACGGCGACA
TGGACATTTA
CATCACCGCC
AGTGGCATCG
ACGACCAGGC
TCTGGAGGAT
GACCGGCTCC
GGAAGTACTA
CCTAGGGAAG
TACCTTCAGG
AGGTGCTGAA
AGCATACCTG
ATTGATAAAG
TCAGAATCAA
AGGCTATTAT
GCATTCAAAC
TGGCTGAAGA
GAAATCTAAA
CCCAGATTTG
GATTCTTCAC
ATCTGATTTT
AAAGCTAAAT
CCTCAATACA
ATTTTACAAC
AAAGTGATCA
GCAGCAGGGG
CTTCCCTTTT
GAGAACAGTA
GTTCTAGATG
CAGTCAGACC
CAAGAAAATA
CAGAGTGCAC
TGTCTGCTTA
TTCCTTGTGC
AGAAGAAACC
ACTGATATTC
CTGGGTTGTT
GCTTCTTCTC
CACCCTGGTT
CTACTCTTAT
CAATTGCCAT
TTTTCAAAGG
CAGAAGAGAA
GAAAGTTTTG
GAAAGCAAAA
AACTTACAAC
ACATACCATT
AAAGAAAGGC
AAGAGAGTTG
TTAGCCTCGA
GGATTACAAG
GACGACGATG
ACAAGTAGGG
ATCCGCGGCC
GCGAAGGATC
TGCGATCGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACGGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGCTG
AAGCTTCGAG
GGGCTCGCAT
CTCTCCTTCA
CGCGCCCGCC
GCCCTACCTG
AGGCCGCCAT
CCACGCCGGT
TGAGTCGCGT
TCTGCCGCCT
CCCGCCTGTG
GTGCCTCCTG
AACTGCGTCC
GCCGTCTAGG
TAAGTTTAAA
GCTCAGGTCG
AGACCGGGCC
TTTGTCCGGC
GCTCCCTTGG
AGCCTACCTA
GACTCAGCCG
GCTCTCCACG
CTTTGCCTGA
CCCTGCTTGC
TCAACTCTAC
GTCTTTGTTT
CGTTTTCTGT
TCTGCGCCGT
TACAGATCCA
AGCTGTGACC
GGCGCCTACG
CTAGATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCCGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGACCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTGAGTCGAC
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAATCAT
CGTCCTTTCC
TTGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAA
ATAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGC
TCTAGCTATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCGGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGACTT
TTGCAGAGAC
GGCCCAAATT
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGGACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCTTTCG
TCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAGACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATATG
CGGTGTGAAA
TACCGCACAG
ATGCGTAAGG
AGAAAATACC
GCATCAGGCG
CCATTCGCCA
TTCAGGCTGC
GCAACTGTTG
GGAAGGGCGA
TCGGTGCGGG
CCTCTTCGCT
ATTACGCCAG
CTGGCGAAAG
GGGGATGTGC
TGCAAGGCGA
TTAAGTTGGG
TAACGCCAGG
GTTTTCCCAG
TCACGACGTT
GTAAAACGAC
GGCCAGTGCC
AAGCTG