名称: | pLV2-Tet-miniCMV-PRAME(human)-3×FLAG-UbC-Neo |
---|---|
别称: | NM_006115.5;CT130/MAPE/OIP-4/OIP4 |
基因长: | 1527bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | Tet-miniCMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 30度 |
背景载体: | pInducer20 |
正向引物: | pFLAG-CMV-F |
反向引物: | |
诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCGAA
TTCACAAATG
GCAGTATTCA
TCCACAATTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTCGGGT
TTATTACAGG
GACAGCAGAG
ATCCAGTTTG
GACTAGGATC
CTTTACCACT
CCCTATCAGT
GATAGAGAAA
AGTGAAAGTC
GAGTTTACCA
CTCCCTATCA
GTGATAGAGA
AAAGTGAAAG
TCGAGTTTAC
CACTCCCTAT
CAGTGATAGA
GAAAAGTGAA
AGTCGAGTTT
ACCACTCCCT
ATCAGTGATA
GAGAAAAGTG
AAAGTCGAGT
TTACCACTCC
CTATCAGTGA
TAGAGAAAAG
TGAAAGTCGA
GTTTACCACT
CCCTATCAGT
GATAGAGAAA
AGTGAAAGTC
GAGTTTACCA
CTCCCTATCA
GTGATAGAGA
AAAGTGAAAG
TCGAGCTCGG
TACCCGGGTC
GAGGTAGGCG
TGTACGGTGG
GAGGCCTATA
TAAGCAGAGC
TCGTTTAGTG
AACCGTCAGA
TCGCCTGGAG
ACGCCATCCA
CGCTGTTTTG
ACCTCCATAG
AAGACACCGG
GACCGATCCA
GCCTCCGCGG
CCCCGAACTA
GTCCAGTGTG
GTGGAATTCT
GCAGATATCA
ACAAGTTTGT
ACAAAAAAGC
TGAACGAGAA
ACGTAAAATG
ATATAAATAT
CAATATATTA
AATTAGATTT
TGCATAAAAA
ACAGACTACA
TAATACTGTA
AAACACAACA
TATCCAGTCA
CTATGGCCAC
CATGGAACGA
AGGCGTTTGT
GGGGTTCCAT
TCAGAGCCGA
TACATCAGCA
TGAGTGTGTG
GACAAGCCCA
CGGAGACTTG
TGGAGCTGGC
AGGGCAGAGC
CTGCTGAAGG
ATGAGGCCCT
GGCCATTGCC
GCCCTGGAGT
TGCTGCCCAG
GGAGCTCTTC
CCGCCACTCT
TCATGGCAGC
CTTTGACGGG
AGACACAGCC
AGACCCTGAA
GGCAATGGTG
CAGGCCTGGC
CCTTCACCTG
CCTCCCTCTG
GGAGTGCTGA
TGAAGGGACA
ACATCTTCAC
CTGGAGACCT
TCAAAGCTGT
GCTTGATGGA
CTTGATGTGC
TCCTTGCCCA
GGAGGTTCGC
CCCAGGAGGT
GGAAACTTCA
AGTGCTGGAT
TTACGGAAGA
ACTCTCATCA
GGACTTCTGG
ACTGTATGGT
CTGGAAACAG
GGCCAGTCTG
TACTCATTTC
CAGAGCCAGA
AGCAGCTCAG
CCCATGACAA
AGAAGCGAAA
AGTAGATGGT
TTGAGCACAG
AGGCAGAGCA
GCCCTTCATT
CCAGTAGAGG
TGCTCGTAGA
CCTGTTCCTC
AAGGAAGGTG
CCTGTGATGA
ATTGTTCTCC
TACCTCATTG
AGAAAGTGAA
GCGAAAGAAA
AATGTACTAC
GCCTGTGCTG
TAAGAAGCTG
AAGATTTTTG
CAATGCCCAT
GCAGGATATC
AAGATGATCC
TGAAAATGGT
GCAGCTGGAC
TCTATTGAAG
ATTTGGAAGT
GACTTGTACC
TGGAAGCTAC
CCACCTTGGC
GAAATTTTCT
CCTTACCTGG
GCCAGATGAT
TAATCTGCGT
AGACTCCTCC
TCTCCCACAT
CCATGCATCT
TCCTACATTT
CCCCGGAGAA
GGAAGAGCAG
TATATCGCCC
AGTTCACCTC
TCAGTTCCTC
AGTCTGCAGT
GCCTGCAGGC
TCTCTATGTG
GACTCTTTAT
TTTTCCTTAG
AGGCCGCCTG
GATCAGTTGC
TCAGGCACGT
GATGAACCCC
TTGGAAACCC
TCTCAATAAC
TAACTGCCGG
CTTTCGGAAG
GGGATGTGAT
GCATCTGTCC
CAGAGTCCCA
GCGTCAGTCA
GCTAAGTGTC
CTGAGTCTAA
GTGGGGTCAT
GCTGACCGAT
GTAAGTCCCG
AGCCCCTCCA
AGCTCTGCTG
GAGAGAGCCT
CTGCCACCCT
CCAGGACCTG
GTCTTTGATG
AGTGTGGGAT
CACGGATGAT
CAGCTCCTTG
CCCTCCTGCC
TTCCCTGAGC
CACTGCTCCC
AGCTTACGAC
CTTAAGCTTC
TACGGGAATT
CCATCTCCAT
ATCTGCCCTG
CAGAGTCTCC
TGCAGCACCT
CATCGGGCTG
AGCAATCTGA
CCCACGTGCT
GTATCCTGTC
CCCCTGGAGA
GTTATGAGGA
CATCCATGGT
ACCCTCCACC
TGGAGAGGCT
TGCCTATCTG
CATGCCAGGC
TCAGGGAGTT
GCTGTGTGAG
TTGGGGCGGC
CCAGCATGGT
CTGGCTTAGT
GCCAACCCCT
GTCCTCACTG
TGGGGACAGA
ACCTTCTATG
ACCCGGAGCC
CATCCTGTGC
CCCTGTTTCA
TGCCTAATGA
CTACAAAGAC
CATGACGGTG
ATTATAAAGA
TCATGACATC
GACTACAAGG
ATGACGATGA
CAAGTAGCAT
AGTGACTGGA
TATGTTGTGT
TTTACAGTAT
TATGTAGTCT
GTTTTTTATG
CAAAATCTAA
TTTAATATAT
TGATATTTAT
ATCATTTTAC
GTTTCTCGTT
CAGCTTTCTT
GTACAAAGTG
GTTGATATCC
AGCACAGTGG
CGGCCGCTCG
AGTCTAGAGG
GCCCGCGGTT
CGAATACCCA
TACGACGTCC
CAGACTACGC
TTAGTAATGA
TTAATTAAAC
TAGAAATTCT
ACCGGGTAGG
GGAGGCGCTT
TTCCCAAGGC
AGTCTGGAGT
AACCCACCCA
AGATCTGGCC
TCCGCGCCGG
GTTTTGGCGC
CTCCCGCGGG
CGCCCCCCTC
CTCACGGCGA
GCGCTGCCAC
GTCAGACGAA
GGGCGCAGCG
AGCGTCCTGA
TCCTTCCGCC
CGGACGCTCA
GGACAGCGGC
CCGCTGCTCA
TAAGACTCGG
CCTTAGAACC
CCAGTATCAG
CAGAAGGACA
TTTTAGGACG
GGACTTGGGT
GACTCTAGGG
CACTGGTTTT
CTTTCCAGAG
AGCGGAACAG
GCGAGGAAAA
GTAGTCCCTT
CTCGGCGATT
CTGCGGAGGG
ATCTCCGTGG
GGCGGTGAAC
GCCGATGATT
ATATAAGGAC
GCGCCGGGTG
TGGCACAGCT
AGTTCCGTCG
CAGCCGGGAT
TTGGGTCGCG
GTTCTTGTTT
GTGGATCGCT
GTGATCGTCA
CTTGGTGAGT
AGCGGGCTGC
TGGGCTGGCC
GGGGCTTTCG
TGGCCGCCGG
GCCGCTCGGT
GGGACGGAAG
CGTGTGGAGA
GACCGCCAAG
GGCTGTAGTC
TGGGTCCGCG
AGCAAGGTTG
CCCTGAACTG
GGGGTTGGGG
GGAGCGCAGC
AAAATGGCGG
CTGTTCCCGA
GTCTTGAATG
GAAGACGCTT
GTGAGGCGGG
CTGTGAGGTC
GTTGAAACAA
GGTGGGGGGC
ATGGTGGGCG
GCAAGAACCC
AAGGTCTTGA
GGCCTTCGCT
AATGCGGGAA
AGCTCTTATT
CGGGTGAGAT
GGGCTGGGGC
ACCATCTGGG
GACCCTGACG
TGAAGTTTGT
CACTGACTGG
AGAACTCGGT
TTGTCGTCTG
TTGCGGGGGC
GGCAGTTATG
GCGGTGCCGT
TGGGCAGTGC
ACCCGTACCT
TTGGGAGCGC
GCGCCCTCGT
CGTGTCGTGA
CGTCACCCGT
TCTGTTGGCT
TATAATGCAG
GGTGGGGCCA
CCTGCCGGTA
GGTGTGCGGT
AGGCTTTTCT
CCGTCGCAGG
ACGCAGGGTT
CGGGCCTAGG
GTAGGCTCTC
CTGAATCGAC
AGGCGCCGGA
CCTCTGGTGA
GGGGAGGGAT
AAGTGAGGCG
TCAGTTTCTT
TGGTCGGTTT
TATGTACCTA
TCTTCTTAAG
TAGCTGAAGC
TCCGGTTTTG
AACTATGCGC
TCGGGGTTGG
CGAGTGTGTT
TTGTGAAGTT
TTTTAGGCAC
CTTTTGAAAT
GTAATCATTT
GGGTCAATAT
GTAATTTTCA
GTGTTAGACT
AGTAAATTGT
CCGCTAAATT
CTGGCCGTTT
TTGGCTTTTT
TGTTAGACGA
AGCTTGGTAC
CGAGCTCGGA
TCTCCACCCC
GTACCGGTCC
TGCAGTCGAA
TTCACCATGT
CTAGACTGGA
CAAGAGCAAA
GTCATAAACG
GAGCTCTGGA
ATTACTCAAT
GGTGTCGGTA
TCGAAGGCCT
GACGACAAGG
AAACTCGCTC
AAAAGCTGGG
AGTTGAGCAG
CCTACCCTGT
ACTGGCACGT
GAAGAACAAG
CGGGCCCTGC
TCGATGCCCT
GCCAATCGAG
ATGCTGGACA
GGCATCATAC
CCACTTCTGC
CCCCTGGAAG
GCGAGTCATG
GCAAGACTTT
CTGCGGAACA
ACGCCAAGTC
ATACCGCTGT
GCTCTCCTCT
CACATCGCGA
CGGGGCTAAA
GTGCATCTCG
GCACCCGCCC
AACAGAGAAA
CAGTACGAAA
CCCTGGAAAA
TCAGCTCGCG
TTCCTGTGTC
AGCAAGGCTT
CTCCCTGGAG
AACGCACTGT
ACGCTCTGTC
CGCCGTGGGC
CACTTTACAC
TGGGCTGCGT
ATTGGAGGAA
CAGGAGCATC
AAGTAGCAAA
AGAGGAAAGA
GAGACACCTA
CCACCGATTC
TATGCCCCCA
CTTCTGAGAC
AAGCAATTGA
GCTGTTCGAC
CGGCAGGGAG
CCGAACCTGC
CTTCCTTTTC
GGCCTGGAAC
TAATCATATG
TGGCCTGGAG
AAACAGCTAA
AGTGCGAAAG
CGGCGGGCCG
ACCGACGCCC
TTGACGATTT
TGACTTAGAC
ATGCTCCCAG
CCGATGCCCT
TGACGACTTT
GACCTTGATA
TGCTGCCTGC
TGACGCTCTT
GACGATTTTG
ACCTTGACAT
GCTCCCCGGG
TAACTAAGTA
AGGATCCGCG
GCCGCACTAG
AGGAATTCCG
CCCCTCTCCC
CCCCCCCCCC
CTAACGTTAC
TGGCCGAAGC
CGCTTGGAAT
AAGGCCGGTG
TGCGTTTGTC
TATATGTTAT
TTTCCACCAT
ATTGCCGTCT
TTTGGCAATG
TGAGGGCCCG
GAAACCTGGC
CCTGTCTTCT
TGACGAGCAT
TCCTAGGGGT
CTTTCCCCTC
TCGCCAAAGG
AATGCAAGGT
CTGTTGAATG
TCGTGAAGGA
AGCAGTTCCT
CTGGAAGCTT
CTTGAAGACA
AACAACGTCT
GTAGCGACCC
TTTGCAGGCA
GCGGAACCCC
CCACCTGGCG
ACAGGTGCCT
CTGCGGCCAA
AAGCCACGTG
TATAAGATAC
ACCTGCAAAG
GCGGCACAAC
CCCAGTGCCA
CGTTGTGAGT
TGGATAGTTG
TGGAAAGAGT
CAAATGGCTC
TCCTCAAGCG
TATTCAACAA
GGGGCTGAAG
GATGCCCAGA
AGGTACCCCA
TTGTATGGGA
TCTGATCTGG
GGCCTCGGTG
CACATGCTTT
ACATGTGTTT
AGTCGAGGTT
AAAAAAACGT
CTAGGCCCCC
CGAACCACGG
GGACGTGGTT
TTCCTTTGAA
AAACACGATG
ATAAGCTTAC
CGGTCCACCA
TGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AAGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGAGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGATGTACA
AGTAAAGCGG
CCGCGACTCT
AGATCATAAT
CAGCCATACC
ACATTTGTAG
AGGTTTTACT
TGCTTTAAAA
AACCTCCCAC
ACCTCCCCCT
GAACCTGAAA
CATAAAATGA
ATGCAATTGT
TGTTGTTTAG
TCCCTCCCAA
TTCGATATCA
AGCTTATCGC
GATAGATCCT
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAATCAT
CGTCCTTTCC
TTGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGAGATCCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
GTAGTTCATG
TCATCTTATT
ATTCAGTATT
TATAACTTGC
AAAGAAATGA
ATATCAGAGA
GTGAGAGGCC
CGGGTTAATT
AAGGAAAGGG
CTAGATCATT
CTTGAAGACG
AAAGGGCCTC
GTGATACGCC
TATTTTTATA
GGTTAATGTC
ATGATAATAA
TGGTTTCTTA
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTG
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTTCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAGAGC
GCCCAATACG
CAAACCGCCT
CTCCCCGCGC
GTTGGCCGAT
TCATTAATGC
AGCAAGCTCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TCCCCGTGGC
ACGACAGGTT
TCCCGACTGG
AAAGCGGGCA
GTGAGCGCAA
CGCAATTAAT
GTGAGTTAGC
TCACTCATTA
GGCACCCCAG
GCTTTACACT
TTATGCTTCC
GGCTCGTATG
TTGTGTGGAA
TTGTGAGCGG
ATAACAATTT
CACACAGGAA
ACAGCTATGA
CATGATTACG
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GATCAACTGG
ATAACTCAAG
CTAACCAAAA
TCATCCCAAA
CTTCCCACCC
CATACCCTAT
TACCACTGCC
AATTACCTGT
GGTTTCATTT
ACTCTAAACC
TGTGATTCCT
CTGAATTATT
TTCATTTTAA
AGAAATTGTA
TTTGTTAAAT
ATGTACTACA
AACTTAGTAG
T