名称: | pLV3-U6-Usp30(mouse)-sgRNA3-Cas9-Puro |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLentiCRISPR V2 |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TATCGATACT
AGTATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTTT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGATTC
TAGAGCTAGC
CTGATACGAA
CTCGGAATTC
GCCACCATGC
CGCCCAAAGC
CCCGCGCAGA
GCCGCGGCCG
CCGAGCCCCC
GCCACCGCCG
CCGCCGCCGC
CTCGGGAGGA
CGACCCCGCG
CAGGACAGCG
GCCCCGAAGA
GCTGCCCCTG
GCCAGGCTTG
AGTTTGAAGA
AATTGAAGAA
CCCGAATTTA
TTGCATTATG
TCAAAAGTTA
AAGGTACCCG
ATCATGTCAG
AGAAAGAGCT
TGGCTAACTT
GGGAGAAAGT
TTCATCCGTG
GATGGAATCC
TGGAAGGATA
TATTCAAAAG
AAGAAGGAAC
TCTGGGGCAT
CTGCATCTTT
ATCGCAGCAG
TTGATCTAGA
TGAGATGCCA
TTCACTTTTA
CTGAGCTACA
GAAAAGCATA
GAAACCAGTG
TCTATAAATT
CTTTGACTTA
TTAAAAGAAA
TCGATACCAG
TACCAAGGTT
GATAATGCTA
TGTCAAGACT
ATTGAAGAAG
TATAATGTGT
TATGTGCACT
CTACAGCAAA
TTAGAACGGA
CGTGTGAACT
TATATATTTG
ACACAACCCA
GCAGTGCGTT
ATCTACTGAA
ATAAATTCTA
TGTTGGTGCT
AAAAATTTCT
TGGATCACTT
TTTTACTAGC
TAAAGGAGAA
GTATTACAGA
TGGAAGATGA
CCTGGTAATC
TCATTTCAGC
TAATGTTGTG
TGTAGTTGAC
TATTTTATTA
AGTTCTCACC
TCCTGCACTA
CTCAGAGAGC
CATACAAAAC
AGCTGCAATC
CCCATTAATG
GTTCACCTCG
AACACCCAGA
AGAGGTCAGA
ACAGGAGCGC
TCGGATAGCA
AAACAACTAG
AAAATGATAC
GAGGATTATC
GAGGTTCTCT
GTAAAGAACA
CGAGTGTAAT
ATAGATGAGG
TGAAAAATGT
TTATTTCAAA
AATTTTATCC
CTTTTATAAA
TTCACTTGGA
ATTGTATCAT
CTAATGGACT
TCCAGAGGTT
GAAAGTCTTT
CTAAACGCTA
TGAAGAAGTT
TATCTTAAAA
ACAAAGATTT
AGATGCAAGA
CTGTTTTTGG
ATCATGATAA
AACACTTCAG
ACTGATCCTA
TAGACAGTTT
TGAAACAGAG
AGAACGCCAC
GAAAAAACAA
CCCTGATGAA
GAGGCAAACG
TGGTTACTCC
ACACACTCCA
GTTAGGACTG
TTATGAATAC
TATTCAACAA
TTAATGGTGA
TTTTAAATTC
TGCAAGTGAT
CAGCCATCAG
AAAATCTGAT
TTCCTACTTC
AATAATTGCA
CAGTGAATCC
AAAAGAAAAT
ATCCTAAAGA
GAGTAAAGGA
TGTTGGGCAC
ATCTTTAAAG
AGAAGTTTGC
TAACGCTGTG
GGCCAGGGCT
GTGTTGACAT
CGGAGTACAG
CGATATAAAC
TTGGAGTCCG
ATTGTATTAC
CGTGTGATGG
AATCCATGCT
TAAATCAGAA
GAAGAACGTT
TGTCCATTCA
GAATTTTAGC
AAACTCCTAA
ATGACAACAT
CTTTCATATG
TCTTTACTGG
CCTGTGCTCT
TGAAGTTGTA
ATGGCTACGT
ATAGCAGAAG
TACATTGCAG
CATCTTGATT
CTGGAACAGA
TTTGTCCTTC
CCGTGGATTC
TGAACGTACT
TAATTTAAAA
GCCTTTGATT
TTTACAAAGT
GATTGAAAGT
TTTATCAAAG
TGGAAGCCAA
CTTGACAAGA
GAAATGATAA
AACATTTAGA
AAGATGTGAG
CATCGAATCA
TGGAATCCCT
TGCATGGCTT
TCAGATTCAC
CTTTATTTGA
TCTCATTAAG
CAGTCCAAGG
ATGGAGAAGG
ACCTGATAAC
CTTGAACCTG
CTTGTCCTCT
CAGCCTGCCT
CTCCAGGGTA
ACCATACTGC
AGCAGATATG
TATCTTTCTC
CTCTAAGATC
TCCAAAGAAA
AGAACTTCCA
CTACACGTGT
AAATTCTGCT
GCAAATACAG
AGACACAAGC
AGCCTCAGCC
TTCCATACTC
AGAAGCCATT
GAAATCTACC
TCCCTTGCCC
TGTTTTACAA
AAAAGTGTAC
CGTCTAGCAT
ATCTCCGACT
AAATACACTC
TGTGCACGCC
TTCTGTCTGA
CCACCCAGAG
CTAGAGCACA
TCATCTGGAC
TCTGTTTCAG
CATACATTGC
AAAATGAGTA
TGAGCTCATG
AGAGACCGAC
ATTTGGACCA
GATTATGATG
TGCTCTATGT
ATGGCATCTG
CAAGGTGAAG
AACATCGACC
TCAAGTTCAA
AATCATCGTC
ACTGCCTACA
AGGATCTTCC
TCACGCTGCC
CAGGAGACCT
TTAAACGTGT
TTTGATCAGA
GAAGAGGAGT
TTGATTCCAT
TATAGTATTC
TATAACTCCG
TTTTCATGCA
GAGACTAAAA
ACAAATATTT
TACAGTATGC
CTCCACCAGG
CCTCCTACCT
TGTCACCAAT
ACCTCACATT
CCTCGAAGCC
CTTACAAGTT
TTCTAGTTCA
CCCTTACGGA
TTCCTGGAGG
TAACATCTAT
ATATCACCCC
TAAAGAGTCC
TTATAAAATT
TCAGAAGGTC
TGCCAACACC
CACAAAAATG
ACTCCGAGAT
CAAGAATCTT
GGTCTCAATT
GGTGAATCAT
TTGGGACATC
TGAAAAGTTC
CAGAAAATAA
ACCAGATGGT
GTGTAATAGT
GACAGAGTGC
TCAAAAGAAG
TGCTGAAGGC
GGCAACCCCC
CCAAACCACT
GAAAAAGCTG
CGCTTTGACA
TCGAGGGAGC
CGATGAAGCA
GATGGGAGTA
AACATCTCCC
AGCGGAGTCC
AAATTCCAAC
AGAAACTGGC
AGAAATGACT
TCCACTCGAA
CACGAATGCA
AAAGCAGAGA
ATGAATGAGA
GCAAGGATGT
CTCAAACAAG
GAGGAAAAGT
GAGGATCCCT
GCGATATCCA
GCGCGGCCGC
GAAGGATCTG
CGATCGCTCC
GGTGCCCGTC
AGTGGGCAGA
GCGCACATCG
CCCACAGTCC
CCGAGAAGTT
GGGGGGAGGG
GTCGGCAATT
GAACGGGTGC
CTAGAGAAGG
TGGCGCGGGG
TAAACTGGGA
AAGTGATGTC
GTGTACTGGC
TCCGCCTTTT
TCCCGAGGGT
GGGGGAGAAC
CGTATATAAG
TGCAGTAGTC
GCCGTGAACG
TTCTTTTTCG
CAACGGGTTT
GCCGCCAGAA
CACAGCTGAA
GCTTCGAGGG
GCTCGCATCT
CTCCTTCACG
CGCCCGCCGC
CCTACCTGAG
GCCGCCATCC
ACGCCGGTTG
AGTCGCGTTC
TGCCGCCTCC
CGCCTGTGGT
GCCTCCTGAA
CTGCGTCCGC
CGTCTAGGTA
AGTTTAAAGC
TCAGGTCGAG
ACCGGGCCTT
TGTCCGGCGC
TCCCTTGGAG
CCTACCTAGA
CTCAGCCGGC
TCTCCACGCT
TTGCCTGACC
CTGCTTGCTC
AACTCTACGT
CTTTGTTTCG
TTTTCTGTTC
TGCGCCGTTA
CAGATCCAAG
CTGTGACCGG
CGCCTACGCT
AGACGCCACC
ATGGAGAGCG
ACGAGAGCGG
CCTGCCCGCC
ATGGAGATCG
AGTGCCGCAT
CACCGGCACC
CTGAACGGCG
TGGAGTTCGA
GCTGGTGGGC
GGCGGAGAGG
GCACCCCCAA
GCAGGGCCGC
ATGACCAACA
AGATGAAGAG
CACCAAAGGC
GCCCTGACCT
TCAGCCCCTA
CCTGCTGAGC
CACGTGATGG
GCTACGGCTT
CTACCACTTC
GGCACCTACC
CCAGCGGCTA
CGAGAACCCC
TTCCTGCACG
CCATCAACAA
CGGCGGCTAC
ACCAACACCC
GCATCGAGAA
GTACGAGGAC
GGCGGCGTGC
TGCACGTGAG
CTTCAGCTAC
CGCTACGAGG
CCGGCCGCGT
GATCGGCGAC
TTCAAGGTGG
TGGGCACCGG
CTTCCCCGAG
GACAGCGTGA
TCTTCACCGA
CAAGATCATC
CGCAGCAACG
CCACCGTGGA
GCACCTGCAC
CCCATGGGCG
ATAACGTGCT
GGTGGGCAGC
TTCGCCCGCA
CCTTCAGCCT
GCGCGACGGC
GGCTACTACA
GCTTCGTGGT
GGACAGCCAC
ATGCACTTCA
AGAGCGCCAT
CCACCCCAGC
ATCCTGCAGA
ACGGGGGCCC
CATGTTCGCC
TTCCGCCGCG
TGGAGGAGCT
GCACAGCAAC
ACCGAGCTGG
GCATCGTGGA
GTACCAGCAC
GCCTTCAAGA
CCCCCATCGC
CTTCGCCAGA
TCCCGCGCTC
AGTCGTCCAA
TTCTGCCGTG
GACGGCACCG
CCGGACCCGG
CTCCACCGGA
TCTCGCGAGG
GCAGAGGAAG
TCTTCTAACA
TGCGGTGACG
TGGAGGAGAA
TCCCGGCCCT
ATGACCGAGT
ACAAGCCCAC
GGTGCGCCTC
GCCACCCGCG
ACGACGTCCC
CAGGGCCGTA
CGCACCCTCG
CCGCCGCGTT
CGCCGACTAC
CCCGCCACGC
GCCACACCGT
CGATCCGGAC
CGCCACATCG
AGCGGGTCAC
CGAGCTGCAA
GAACTCTTCC
TCACGCGCGT
CGGGCTCGAC
ATCGGCAAGG
TGTGGGTCGC
GGACGACGGC
GCCGCGGTGG
CGGTCTGGAC
CACGCCGGAG
AGCGTCGAAG
CGGGGGCGGT
GTTCGCCGAG
ATCGGCCCGC
GCATGGCCGA
GTTGAGCGGT
TCCCGGCTGG
CCGCGCAGCA
ACAGATGGAA
GGCCTCCTGG
CGCCGCACCG
GCCCAAGGAG
CCCGCGTGGT
TCCTGGCCAC
CGTCGGCGTC
TCGCCCGACC
ACCAGGGCAA
GGGTCTGGGC
AGCGCCGTCG
TGCTCCCCGG
AGTGGAGGCG
GCCGAGCGCG
CCGGGGTGCC
CGCCTTCCTG
GAGACCTCCG
CGCCCCGCAA
CCTCCCCTTC
TACGAGCGGC
TCGGCTTCAC
CGTCACCGCC
GACGTCGAGG
TGCCCGAAGG
ACCGCGCACC
TGGTGCATGA
CCCGCAAGCC
CGGTGCCTGA
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAGCTGA
CGTCCTTTCC
ATGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTC
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGCCGC
CTCCCCGCCT
GGTACCTTTA
AGACCAATGA
CTTACAAGGC
AGCTGTAGAT
CTTAGCCACT
TTTTAAAAGA
AAAGGGGGGA
CTGGAAGGGC
TAATTCACTC
CCAACGAAAA
TAAGATCTGC
TTTTTGCTTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTAGT
AGTTCATGTC
ATCTTATTAT
TCAGTATTTA
TAACTTGCAA
AGAAATGAAT
ATCAGAGAGT
GAGAGGAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGGCT
CTAGCTATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGACTTT
TGCAGAGACG
GCCCAAATTC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGGACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCTAA
GAAACCATTA
TTATCATGAC
ATTAACCTAT
AAAAATAGGC
GTATCACGAG
GCCCTTTCGT
CTCGCGCGTT
TCGGTGATGA
CGGTGAAAAC
CTCTGACACA
TGCAGCTCCC
GGAGACGGTC
ACAGCTTGTC
TGTAAGCGGA
TGCCGGGAGC
AGACAAGCCC
GTCAGGGCGC
GTCAGCGGGT
GTTGGCGGGT
GTCGGGGCTG
GCTTAACTAT
GCGGCATCAG
AGCAGATTGT
ACTGAGAGTG
CACCATATGC
GGTGTGAAAT
ACCGCACAGA
TGCGTAAGGA
GAAAATACCG
CATCAGGCGC
CATTCGCCAT
TCAGGCTGCG
CAACTGTTGG
GAAGGGCGAT
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGC
TGGCGAAAGG
GGGATGTGCT
GCAAGGCGAT
TAAGTTGGGT
AACGCCAGGG
TTTTCCCAGT
CACGACGTTG
TAAAACGACG
GCCAGTGCCA
AGCTG