名称: | pPGK-Fluc-CCND2(human)-3UTR-Rluc-Neo |
---|---|
别称: | · |
基因长: | 5344bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 荧光素酶检测 |
片段类型: | UTR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | Fluc,Rluc |
启动子: | PGK |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pmirGLO |
正向引物: | luc-F2 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATGCAAGCT
GATCCGGCTG
CTAACAAAGC
CCGAAAGGAA
GCTGAGTTGG
CTGCTGCCAC
CGCTGAGCAA
TAACTAGCAT
AACCCCTTGG
GGCGGCCGCT
TCGAGCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTAACA
ACAACAATTG
CATTCATTTT
ATGTTTCAGG
TTCAGGGGGA
GATGTGGGAG
GTTTTTTTAA
GCAAGTAAAA
CCTCTACAAA
TGTGGTAAAA
TCGAATTTTA
ACAAAATATT
AACGCTTACA
ATTTCCTGAT
GCGGTATTTT
CTCCTTACGC
ATCTGTGCGG
TATTTCACAC
CGCATACGCG
GATCTGCGCA
GCACCATGGC
CTGAAATAAC
CTCTGAAAGA
GGAACTTGGT
TAGGTACCTT
CTGAGGCGGA
AAGAACCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTTGATTCT
TCTGACACAA
CAGTCTCGAA
CCAAAGGCTG
GAGCCACCAT
GGCTTCCAAG
GTGTACGACC
CCGAGCAACG
CAAACGCATG
ATCACTGGGC
CTCAGTGGTG
GGCTCGCTGC
AAGCAAATGA
ACGTGCTGGA
CTCCTTCATC
AACTACTATG
ATTCCGAGAA
GCACGCCGAG
AACGCCGTGA
TTTTTCTGCA
TGGTAACGCT
GCCTCCAGCT
ACCTGTGGAG
GCACGTCGTG
CCTCACATCG
AGCCCGTGGC
TAGATGCATC
ATCCCTGATC
TGATCGGAAT
GGGTAAGTCC
GGCAAGAGCG
GGAATGGCTC
ATATCGCCTC
CTGGATCACT
ACAAGTACCT
CACCGCTTGG
TTCGAGCTGC
TGAACCTTCC
AAAGAAAATC
ATCTTTGTGG
GCCACGACTG
GGGGGCTTGT
CTGGCCTTTC
ACTACTCCTA
CGAGCACCAA
GACAAGATCA
AGGCCATCGT
CCATGCTGAG
AGTGTCGTGG
ACGTGATCGA
GTCCTGGGAC
GAGTGGCCTG
ACATCGAGGA
GGATATCGCC
CTGATCAAGA
GCGAAGAGGG
CGAGAAAATG
GTGCTTGAGA
ATAACTTCTT
CGTCGAGACC
ATGCTCCCAA
GCAAGATCAT
GCGGAAACTG
GAGCCTGAGG
AGTTCGCTGC
CTACCTGGAG
CCATTCAAGG
AGAAGGGCGA
GGTTAGACGG
CCTACCCTCT
CCTGGCCTCG
CGAGATCCCT
CTCGTTAAGG
GAGGCAAGCC
CGACGTCGTC
CAGATTGTCC
GCAACTACAA
CGCCTACCTT
CGGGCCAGCG
ACGATCTGCC
TAAGATGTTC
ATCGAGTCCG
ACCCTGGGTT
CTTTTCCAAC
GCTATTGTCG
AGGGAGCTAA
GAAGTTCCCT
AACACCGAGT
TCGTGAAGGT
GAAGGGCCTC
CACTTCAGCC
AGGAGGACGC
TCCAGATGAA
ATGGGTAAGT
ACATCAAGAG
CTTCGTGGAG
CGCGTGCTGA
AGAACGAGCA
GACCGGTGGT
GGGAGCGGAG
GTGGCGGATC
AGGTGGCGGA
GGCTCCGGAG
GGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AGGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGCGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
TGGCCGCAAT
AAAATATCTT
TATTTTCATT
ACATCTGTGT
GTTGGTTTTT
TGTGTGAATC
GATAGCGATA
AGGATCCTCT
TTGCGCTTGC
GTTTTCCCTT
GTCCAGATAG
CCCAGTAGCT
GACATTCATC
CGGGGTCAGC
ACCGTTTCTG
CGGACTGGCT
TTCTACGTAA
TGGTTTCTTA
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTCAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACTATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAATTC
GAAATGACCG
ACCAAGCGAC
GCCCAACCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATTT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTATAGTCC
GGAAATACAG
GAACGCACGC
TGGATGGCCC
TTCGCTGGGA
TGGTGAAACC
ATGAAAAATG
GCAGCTTCAG
TGGATTAAGT
GGGGGTAATG
TGGCCTGTAC
CCTCTGGTTG
CATAGGTATT
CATACGGTTA
AAATTTATCA
GGCGCGATTG
CGGCAGTTTT
TCGGGTGGTT
TGTTGCCATT
TTTACCTGTC
TGCTGCCGTG
ATCGCGCTGA
ACGCGTTTTA
GCGGTGCGTA
CAATTAAGGG
ATTATGGTAA
ATCCACTTAC
TGTCTGCCCT
CGTAGCCATC
GAGATAAACC
GCAGTACTCC
GGCCACGATG
CGTCCGGCGT
AGAGGATCGA
GATCTACCGG
GTAGGGGAGG
CGCTTTTCCC
AAGGCAGTCT
GGAGCATGCG
CTTTAGCAGC
CCCGCTGGGC
ACTTGGCGCT
ACACAAGTGG
CCTCTGGCCT
CGCACACATT
CCACATCCAC
CGGTAGGCGC
CAACCGGCTC
CGTTCTTTGG
TGGCCCCTTC
GCGCCACCTT
CTACTCCTCC
CCTAGTCAGG
AAGTTCCCCC
CCGCCCCGCA
GCTCGCGTCG
TGCAGGACGT
GACAAATGGA
AGTAGCACGT
CTCACTAGTC
TCGTGCAGAT
GGACAGCACC
GCTGAGCAAT
GGAAGCGGGT
AGGCCTTTGG
GGCAGCGGCC
AATAGCAGCT
TTGCTCCTTC
GCTTTCTGGG
CTCAGAGGCT
GGGAAGGGGT
GGGTCCGGGG
GCGGGCTCAG
GGGCGGGCTC
AGGGGCGGGG
CGGGCGCCCG
AAGGTCCTCC
GGAGGCCCGG
CATTCTGCAC
GCTTCAAAAG
CGCACGTCTG
CCGCGCTGTT
CTCCTCTTCC
TCATCTCCGG
GCCTTTCGAC
CTGCAGCCCA
AGCTTGGCAA
TCCGGTACTG
TTGGTAAAGC
CACCATGGAA
GATGCCAAAA
ACATTAAGAA
GGGCCCAGCG
CCATTCTACC
CACTCGAAGA
CGGGACCGCC
GGCGAGCAGC
TGCACAAAGC
CATGAAGCGC
TACGCCCTGG
TGCCCGGCAC
CATCGCCTTT
ACCGACGCAC
ATATCGAGGT
GGACATTACC
TACGCCGAGT
ACTTCGAGAT
GAGCGTTCGG
CTGGCAGAAG
CTATGAAGCG
CTATGGGCTG
AATACAAACC
ATCGGATCGT
GGTGTGCAGC
GAGAATAGCT
TGCAGTTCTT
CATGCCCGTG
TTGGGTGCCC
TGTTCATCGG
TGTGGCTGTG
GCCCCAGCTA
ACGACATCTA
CAACGAGCGC
GAGCTGCTGA
ACAGCATGGG
CATCAGCCAG
CCCACCGTCG
TATTCGTGAG
CAAGAAAGGG
CTGCAAAAGA
TCCTCAACGT
GCAAAAGAAG
CTACCGATCA
TACAAAAGAT
CATCATCATG
GATAGCAAGA
CCGACTACCA
GGGCTTCCAA
AGCATGTACA
CCTTCGTGAC
TTCCCATTTG
CCACCCGGCT
TCAACGAGTA
CGACTTCGTG
CCCGAGAGCT
TCGACCGGGA
CAAAACCATC
GCCCTGATCA
TGAACAGTAG
TGGCAGTACC
GGATTGCCCA
AGGGCGTAGC
CCTACCGCAC
CGCACCGCTT
GTGTCCGATT
CAGTCATGCC
CGCGACCCCA
TCTTCGGCAA
CCAGATCATC
CCCGACACCG
CTATCCTCAG
CGTGGTGCCA
TTTCACCACG
GCTTCGGCAT
GTTCACCACG
CTGGGCTACT
TGATCTGCGG
CTTTCGGGTC
GTGCTCATGT
ACCGCTTCGA
GGAGGAGCTA
TTCTTGCGCA
GCTTGCAAGA
CTATAAGATT
CAATCTGCCC
TGCTGGTGCC
CACACTATTT
AGCTTCTTCG
CTAAGAGCAC
TCTCATCGAC
AAGTACGACC
TAAGCAACTT
GCACGAGATC
GCCAGCGGCG
GGGCGCCGCT
CAGCAAGGAG
GTAGGTGAGG
CCGTGGCCAA
ACGCTTCCAC
CTACCAGGCA
TCCGCCAGGG
CTACGGCCTG
ACAGAAACAA
CCAGCGCCAT
TCTGATCACC
CCCGAAGGGG
ACGACAAGCC
TGGCGCAGTA
GGCAAGGTGG
TGCCCTTCTT
CGAGGCTAAG
GTGGTGGACT
TGGACACCGG
TAAGACACTG
GGTGTGAACC
AGCGCGGCGA
GCTGTGCGTC
CGTGGCCCCA
TGATCATGAG
CGGCTACGTT
AACAACCCCG
AGGCTACAAA
CGCTCTCATC
GACAAGGACG
GCTGGCTGCA
CAGCGGCGAC
ATCGCCTACT
GGGACGAGGA
CGAGCACTTC
TTCATCGTGG
ACCGGCTGAA
GAGCCTGATC
AAATACAAGG
GCTACCAGGT
AGCCCCAGCC
GAACTGGAGA
GCATCCTGCT
GCAACACCCC
AACATCTTCG
ACGCCGGGGT
CGCCGGCCTG
CCCGACGACG
ATGCCGGCGA
GCTGCCCGCC
GCAGTCGTCG
TGCTGGAACA
CGGTAAAACC
ATGACCGAGA
AGGAGATCGT
GGACTATGTG
GCCAGCCAGG
TTACAACCGC
CAAGAAGCTG
CGCGGTGGTG
TTGTGTTCGT
GGACGAGGTG
CCTAAAGGAC
TGACCGGCAA
GTTGGACGCC
CGCAAGATCC
GCGAGATTCT
CATTAAGGCC
AAGAAGGGCG
GCAAGATCGC
CGTGTAATTC
TAGTTGTTTA
AACGAGCTCG
CTAGCGGATG
CCAGTTGGGC
CGAAAGAGAG
AGACGCGTCC
ATAATCTGGT
CTCTTCTTCT
TTCTGGTTGT
TTTTGTTCTT
TGTGTTTTAG
GGTGAAACTT
AAAAAAAAAA
TTCTGCCCCC
ACCTAGATCA
TATTTAAAGA
TCTTTTAGAA
GTGAGAGAAA
AAGGTCCTAC
GAAAACGGAA
TAATAAAAAG
CATTTGGTGC
CTATTTGAAG
TACAGCATAA
GGGAATCCCT
TGTATATGCG
AACAGTTATT
GTTTGATTAT
GTAAAAGTAA
TAGTAAAATG
CTTACAGGAA
AACCTGCAGA
GTAGTTAGAG
AATATGTATG
CCTGCAATAT
GGGAACAAAT
TAGAGGAGAC
TTTTTTTTTT
CATGTTATGA
GCTAGCACAT
ACACCCCCTT
GTAGTATAAT
TTCAAGGAAC
TGTGTACGCC
ATTTATGGCA
TGATTAGATT
GCAAAGCAAT
GAACTCAAGA
AGGAATTGAA
ATAAGGAGGG
ACATGATGGG
GAAGGAGTAC
AAAACAATCT
CTCAACATGA
TTGAACCATT
TGGGATGGAG
AAGCACCTTT
GCTCTCAGCC
ACCTGTTACT
AAGTCAGGAG
TGTAGTTGGA
TCTCTACATT
AATGTCCTCT
TGCTGTCTAC
AGTAGCTGCT
ACCTAAAAAA
AGATGTTTTA
TTTTGCCAGT
TGGACACAGG
TGATTGGCTC
CTGGGTTTCA
TGTTCTGTGA
CATCCTGCTT
CTTCTTCCAA
ATGCAGTTCA
TTGCAGACAC
CACCATATTG
CTATCTAATG
GGGAAATGTA
GCTATGGGCC
ATAACCAAAA
CTCACATGAA
ACGGAGGCAG
ATGGAGACCA
AGGGTGGGAT
CCAGAATGGA
GTCTTTTCTG
TTATTGTATT
TAAAAGGGTA
ATGTGGCCTT
GGCATTTCTT
CTTAGAAAAA
AACTAATTTT
TGGTGCTGAT
TGGCATGTCT
GGTTCACAGT
TTAGCATTGT
TATAAACCAT
TCCATTCGAA
AAGCACTTTG
AAAAATTGTT
CCCGAGCGAT
AGATGGGATG
GTTTATGCAA
GTCATGCTGA
ATACTCCTCC
CCTCTTCTCT
TTTGCCCCCT
CCCTTCCTGC
CCCCAGTCTG
GGTTACTCTT
CGCTTCTGGT
ATCTGGCGTT
CTTTGGTACA
CAGTTCTGGT
GTTCCTACCA
GGACTCAAGA
GACACCCCTT
CCTGCTGACA
TTCCCATCAC
AACATTCCTC
AGACAAGCCT
GTAAACTAAA
ATCTGTTACC
ATTCTGATGG
CACAGAAGGA
TCTTAATTCC
CATCTCTATA
CTTCTCCTTT
GGACATGGAA
AGAAAAGTTA
TTGCTGGTGC
AAAGATAGAT
GGCTGAACAT
CAGGGTGTGG
CATTTTGTTC
CCTTTTCCGT
TTTTTTTTTT
TTATTGTTGT
TGTTAATTTT
ATTGCAAAGT
TGTATTCAGC
GTACTTGAAT
TTTTCTTCCT
CTCCACTTCT
TAGAGGCATT
CAGTTAGCAA
AGAGGTTGGA
GCAACAACTT
TTTTTTTTTT
TTTTGCACAA
TTGTAATTGA
CAGGTAATGA
AGCTATTTGT
TAAAATATTT
GCCTTTTTAA
GTAAAAAAGA
AAAATCAGAA
CAGGGCTATT
TGAAGAATTA
TTTTATACAC
AGATTCTGCC
TTGTTTCATA
GTATGAGGGT
TGAAGACGGA
AAACAATCTA
AGGGTCTCTC
ATTTTTTTAA
TTTTGTTTTG
TTCAGTTTGG
TTTTTTTTTT
TTTTTGCGCT
GCTAAGAAGC
TAAAGTCATC
CATCCTTATT
CACGTTGACA
GTACCTAGCT
GTAATGTTTC
ACAGAGTGTG
CTGCTATTTT
ATAAACATTT
TTATAATATA
TTATTTTACT
GCTTAAATTC
CAAGTCCTGA
AGTAGATGGT
TGAGATATGA
GTTCTTCGTA
CTGGAAAAGC
CCTTCCGTAG
TTTGTTTTCT
TCTGGTAGCA
TATTCATGGT
TGTTTTTTTT
TTTCTTTTTT
GGTTTTTTGG
TTTTTTTTTT
TTCCTCTGAT
CACATTCTTC
AAAGACGGAG
TATTCTTTAC
CTCAGGTTTA
CTGGACAAAA
TCAATAACTA
CAAAAGGCAA
TGATTCACGC
TTTTGTTTTC
ATAATACCTC
ACAACCGTAC
AGTTTCTGCT
TGGGAGCCCA
TTCGCATGAG
GAATACAGAA
GCAGTGTGAG
CAGGGCTGAC
TCCCTCTCAG
GTGGAAGGCA
GGGCGGTCTC
ACTCCCAGGG
ACCTTTTTGG
TCATGGAGGC
CATCGGGCTC
CCAGTTAGAC
CCTGGTATCC
TCATCATGAT
GGAAAAAATA
CATTGAACCA
AGGGATCCTC
CCTCCCCTTC
AAGGCAGACG
TTCAGTACAA
ACATTTATGC
GGTAGGCTCA
GATGTCGTAA
TTTGCACTTA
GGTACCAGGT
GTCAGGAAAC
AGACTAAAAA
GAATTCCACC
AGGCTGTTTG
GAGATCCTCA
TCTTGGAGCT
TTTTCAAAAG
CGGGGCTTCA
TCTGCAAAGG
GCCCTTTCAT
CTTGAAGTTT
TTCCCCTCCG
TCTTTCCCCT
CCCCTGGCAT
GGACACCTTG
TGTTTAGGAT
CATCTCTGCA
GGTTTCCTAG
GTCTGAATCT
GCGAGTAGAT
GAACCTGCAG
CAAGCAGCGT
TTATGGTGCT
TCCTTCTCCC
TCCTCTGTCT
CAAACTGCGC
AGGCAAGCAC
TATGCAAGCC
CAGGCCCTCT
GCTGAGCGGT
ACTAAACGGT
CGGGTTTTCA
ATCACACTGA
ATTGGCAGGA
TAAGAAAAAT
AGGTCAGATA
AGTATGGGAT
GATAGTTGAA
GGGAGGTGAA
GAGGCTGCTT
CTCTACAGAG
GTGAAATTCC
AGATGAGTCA
GTCTCTTGGG
AAGTGTGTTT
AGAAGGGTTC
AGGACTTTGT
GAGTTAGCAT
GACCCTAAAA
TTCTAGGGGA
TTTCTGGTGG
GACAATGGGT
GGTGAATTCT
GAAGTTTTGG
AGAGGGAAGT
GGAGCAGCCA
GCAAGTAAGC
TAGCCAGAGT
TTTCTCAAGA
GCCAGCTTTG
CTCAGCACAC
TCTCCTGGGC
CCCAAGGAGT
CCCACGGAAT
GGGGAAAGCG
GGAACCCTGG
AGTTCTTGGG
AATCTTGGAG
CCTAAAGAGA
AACCGAGGTG
CAAATTCATT
TCATGGTGAC
TGACCCTTGA
GCTTAAACAG
AAGCAGCAAA
TGAAAGAACC
GGACAAATAA
GGAAGGGCAC
AAGCCTACCC
GACTCTATTT
ACAGTCTGTA
ACTTTCCACT
CTTCCTGTAG
TCCCGAGGCC
CCTGGGTCCT
TCTAGCTTTT
CTCTTTCCCA
TCCTTGGGGC
CTTGTGTGAT
GATGGGTGTG
GGGCTGCCGA
TGGGAAAGTC
GGGGGTTGTT
AGGCTTTTCT
GCCTGCTCCT
GCTTAAACAC
AAGAAGGAAT
CCTGGATTTT
GCCCTCTCCT
TAGCTCTTAG
TCTCTTTGGT
AGGAGTTTTG
TTCCAGAGGA
GCTCTCCCCC
TTGGATTTGA
ACTTGCTCTT
TTTGTTGTTG
TTGTTCTTTC
TCTTCTTTTT
CTTACCTCCC
ACTAAAGGGG
TTCCAAATTA
TCCTGGTCTT
TTTCTACCTT
GTTGTGTTTC
TATCTCGTCT
TTACTTCCAT
CTGTTTGTTT
TTTTCTCCAT
CAGTGGGGGC
CGAGTTGTTC
CCCCAGCCTG
CCAAATTTTG
ATCCTTCCCC
TCTTTTGGCC
AAATCCTAGG
GGGAAGAAAT
CCTAGTATGC
CAAAAATATA
TGCTAAGCAT
AATTAAACTC
CATGCGGGTC
CATAACAGCC
AAGAAGCCTG
CAGGAGAAAG
CCAAGGGCAG
TTCCCTCCGC
AGAACACCCC
ATGCGTGCTG
AGAGGCGAGC
TCCTTGAAGA
AGGGGCTGTT
CTTCCAGGAG
GCCTTATTTT
GAACTGCCTC
AGGACCCCAC
TGGAGAGCAC
AGCATGCCTT
ACTACTGGGT
CATCCTTGGT
CTATGTGCTC
TGTACTGGAG
GCTCTGTTCT
GCCTCTTATC
AGCCAGGTCA
GGGGCACACA
TGGCTTAAGT
GACAAAGCCA
GAGGAGAAGA
CAACCCTGAC
AGCATCACGC
TGCATCCCAT
TGCTAGCAGG
ATTGGCAACT
CTTCAGACGG
AGCTGCGCTT
CCCTGCAGTC
TAGCACCTCT
AGGGCCTCTC
CAGACTGTGC
CCTGGGAGCT
CTGGGACTGA
AAGGTTAAGA
ACATAAGGCA
GGATCAGATG
ACTCTCTCCA
AGAGGGCAGG
GGAATTTTCT
CTCCATGGGC
CACAGGGGAC
AGGGCTGGGA
GAAGAAATAG
ACTTGCACCT
TATGTCATGT
AAATAATTGA
TTTTCTAGTT
CAAGAAGATA
ATATTGGTAG
TGTGGGAATT
GGAGGTAGGA
AGGGGAGGAA
GTCTGAGTAA
GCCAGTTGGC
TTCTAAGCCA
AAAGGATTCC
TCTTTGTTTA
TCTCTGAGAC
AGTCCAACCT
TGAGAATAGC
TTTAAAAGGG
AAATTAATGC
TGAGATGATA
AAGTCCCCTT
AAGCCAACAA
ACCCTCTGTA
GCTATAGAAT
GAGTGCAGGT
TTCTATTGGT
GTGGACTCAG
AGCAATTTAC
AAGAGCTGTT
CATGCAGCCA
TCCATTTGTG
CAAAATAGGG
TAAGAAGATT
CAAGAGGATA
TTTATTACTT
CCTCATACCA
CATGGCTTTT
GATGATTCTG
GATTCTAAAC
AACCCAGAAT
GGTCATTTCA
GGCACAACGA
TACTACATTC
GTGTGTGTCT
GCTTTTAAAC
TTGGCTGGGC
TATCAGACCC
TATTCTCGGC
TCAGGTTTTG
AGAAGCCATC
AGCAAATGTG
TACGTGCATG
CTGTAGCTGC
AGCCTGCATC
CCTTCGCCTG
CAGCCTACTT
TGGGGAAATA
AAGTGCCTTA
CTGACTGTAG
CCATTACAGT
ATCCAATGTC
TTTTGACAGG
TGCCTGTCCT
TGAAAAACAA
AGTTTCTATT
TTTATTTTTA
ATTGGTTTAG
TTCTTAACTG
CTGGCCAACT
CTTACATCCC
CAGCAAATCA
TCGGGCCATT
GGATTTTTTC
CATTATGTTC
ATCACCCTTA
TATCATGTAC
CTCAGATCTC
TCTCTCTCTC
CTCTCTCTCA
GTTATGTAGT
TTCTTGTCTT
GGACTTTTTT
TTTTCTTTTC
TTTTTCTTTT
TTTTTTTGCT
TTAAAACAAG
TGTGATGCCA
TATCAAGTCC
ATGTTATTCT
CTCACAGTGT
ACTCTATAAG
AGGTGTGGGT
GTCTGTTTGG
TCAGGATGTT
AGAAAGTGCT
GATAAGTAGC
ATGATCAGTG
TATGCGAAAA
GGTTTTTAGG
AAGTATGGCA
AAAATGTTGT
ATTGGCTATG
ATGGTGACAT
GATATAGTCA
GCTGCCTTTT
AAGAGGTCTT
ATCTGTTCAG
TGTTAAGTGA
TTTAAAAAAA
TAATAACCTG
TTTTCTGACT
AGTTTAAAGA
TGGATTTGAA
AATGGTTTTG
AATGCAATTA
GGTTATGCTA
TTTGGACAAT
AAACTCACCT
TGACCTAAAT
CTAGAGTCGA
CCTGCAGG