名称: | pGADT7-Ppp1cc(mouse) |
---|---|
别称: | NM_001424537.1;dis2m1; PP-1G; PP1 |
基因长: | 1011bp |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | LEU2 |
荧光标记: | |
启动子: | ADH1 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pGADT7 |
正向引物: | T7 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGCATGCCTG
CAGGTCGAGA
TCCGGGATCG
AAGAAATGAT
GGTAAATGAA
ATAGGAAATC
AAGGAGCATG
AAGGCAAAAG
ACAAATATAA
GGGTCGAACG
AAAAATAAAG
TGAAAAGTGT
TGATATGATG
TATTTGGCTT
TGCGGCGCCG
AAAAAACGAG
TTTACGCAAT
TGCACAATCA
TGCTGACTCT
GTGGCGGACC
CGCGCTCTTG
CCGGCCCGGC
GATAACGCTG
GGCGTGAGGC
TGTGCCCGGC
GGAGTTTTTT
GCGCCTGCAT
TTTCCAAGGT
TTACCCTGCG
CTAAGGGGCG
AGATTGGAGA
AGCAATAAGA
ATGCCGGTTG
GGGTTGCGAT
GATGACGACC
ACGACAACTG
GTGTCATTAT
TTAAGTTGCC
GAAAGAACCT
GAGTGCATTT
GCAACATGAG
TATACTAGAA
GAATGAGCCA
AGACTTGCGA
GACGCGAGTT
TGCCGGTGGT
GCGAACAATA
GAGCGACCAT
GACCTTGAAG
GTGAGACGCG
CATAACCGCT
AGAGTACTTT
GAAGAGGAAA
CAGCAATAGG
GTTGCTACCA
GTATAAATAG
ACAGGTACAT
ACAACACTGG
AAATGGTTGT
CTGTTTGAGT
ACGCTTTCAA
TTCATTTGGG
TGTGCACTTT
ATTATGTTAC
AATATGGAAG
GGAACTTTAC
ACTTCTCCTA
TGCACATATA
TTAATTAAAG
TCCAATGCTA
GTAGAGAAGG
GGGGTAACAC
CCCTCCGCGC
TCTTTTCCGA
TTTTTTTCTA
AACCGTGGAA
TATTTCGGAT
ATCCTTTTGT
TGTTTCCGGG
TGTACAATAT
GGACTTCCTC
TTTTCTGGCA
ACCAAACCCA
TACATCGGGA
TTCCTATAAT
ACCTTCGTTG
GTCTCCCTAA
CATGTAGGTG
GCGGAGGGGA
GATATACAAT
AGAACAGATA
CCAGACAAGA
CATAATGGGC
TAAACAAGAC
TACACCAATT
ACACTGCCTC
ATTGATGGTG
GTACATAACG
AACTAATACT
GTAGCCCTAG
ACTTGATAGC
CATCATCATA
TCGAAGTTTC
ACTACCCTTT
TTCCATTTGC
CATCTATTGA
AGTAATAATA
GGCGCATGCA
ACTTCTTTTC
TTTTTTTTTC
TTTTCTCTCT
CCCCCGTTGT
TGTCTCACCA
TATCCGCAAT
GACAAAAAAA
ATGATGGAAG
ACACTAAAGG
AAAAAATTAA
CGACAAAGAC
AGCACCAACA
GATGTCGTTG
TTCCAGAGCT
GATGAGGGGT
ATCTCGAAGC
ACACGAAACT
TTTTCCTTCC
TTCATTCACG
CACACTACTC
TCTAATGAGC
AACGGTATAC
GGCCTTCCTT
CCAGTTACTT
GAATTTGAAA
TAAAAAAAAG
TTTGCTGTCT
TGCTATCAAG
TATAAATAGA
CCTGCAATTA
TTAATCTTTT
GTTTCCTCGT
CATTGTTCTC
GTTCCCTTTC
TTCCTTGTTT
CTTTTTCTGC
ACAATATTTC
AAGCTATACC
AAGCATACAA
TCAACTCCAA
GCTTTGCAAA
GATGGATAAA
GCGGAATTAA
TTCCCGAGCC
TCCAAAAAAG
AAGAGAAAGG
TCGAATTGGG
TACCGCCGCC
AATTTTAATC
AAAGTGGGAA
TATTGCTGAT
AGCTCATTGT
CCTTCACTTT
CACTAACAGT
AGCAACGGTC
CGAACCTCAT
AACAACTCAA
ACAAATTCTC
AAGCGCTTTC
ACAACCAATT
GCCTCCTCTA
ACGTTCATGA
TAACTTCATG
AATAATGAAA
TCACGGCTAG
TAAAATTGAT
GATGGTAATA
ATTCAAAACC
ACTGTCACCT
GGTTGGACGG
ACCAAACTGC
GTATAACGCG
TTTGGAATCA
CTACAGGGAT
GTTTAATACC
ACTACAATGG
ATGATGTATA
TAACTATCTA
TTCGATGATG
AAGATACCCC
ACCAAACCCA
AAAAAAGAGA
TCTTTAATAC
GACTCACTAT
AGGGCGAGCG
CCGCCATGGA
GTACCCATAC
GACGTACCAG
ATTACGCTCA
TATGGCCATG
GAGGCCAGTG
AATTCATGGC
GGATATCGAC
AAACTCAACA
TCGACAGCAT
CATCCAACGG
CTGCTGGAAG
TGAGAGGGTC
CAAGCCAGGC
AAGAATGTCC
AGCTCCAGGA
GAACGAGATC
CGAGGACTCT
GCCTGAAGTC
TCGGGAGATC
TTCCTCAGTC
AGCCTATCCT
TTTAGAACTT
GAAGCACCAC
TCAAGATATG
TGGTGACATC
CACGGGCAGT
ACTATGATTT
GCTCCGTCTG
TTTGAATACG
GTGGCTTTCC
TCCAGAGAGC
AACTATTTGT
TTCTCGGGGA
CTATGTGGAC
AGGGGCAAGC
AGTCCCTGGA
GACAATCTGC
CTCTTGCTGG
CCTACAAAAT
CAAGTATCCG
GAGAACTTCT
TTCTTCTCAG
AGGGAACCAC
GAGTGCGCCA
GCATCAATAG
GATCTACGGA
TTTTATGATG
AGTGTAAAAG
AAGATACAAC
ATTAAGCTGT
GGAAAACGTT
CACAGACTGT
TTTAACTGCT
TGCCGATAGC
AGCCATCGTG
GACGAGAAGA
TATTCTGCTG
TCATGGAGGT
TTATCACCAG
ATCTTCAATC
TATGGAGCAG
ATTCGGCGAA
TTATGAGACC
AACTGATGTA
CCAGATCAAG
GTCTTCTTTG
TGATCTTTTG
TGGTCTGACC
CCGATAAAGA
TGTCTTAGGC
TGGGGTGAAA
ATGACAGAGG
AGTGTCCTTC
ACATTTGGTG
CAGAAGTGGT
TGCAAAATTT
CTCCATAAGC
ATGATTTGGA
TCTTATATGT
AGAGCCCATC
AGGTGGTTGA
AGATGGCTAT
GAGTTTTTTG
CAAAGAGGCA
GTTAGTCACT
CTGTTTTCTG
CACCCAACTA
CTGTGGCGAG
TTTGACAATG
CAGGCGCCAT
GATGAGTGTG
GATGAGACCC
TCATGTGTTC
CTTCCAGATT
TTAAAGCCTG
CAGAGAAAAA
GAAGCCCAAC
GCCACGAGAC
CTGTCACACC
ACCACGGGTT
GGATCAGGCC
TGAACCCGTC
CATTCAGAAA
GCTTCAAATT
ATAGAAACAA
CACTGTCCTA
TACGAGTGAG
GATCCATCGA
GCTCGAGCTG
CAGATGAATC
GTAGATACTG
AAAAACCCCG
CAAGTTCACT
TCAACTGTGC
ATCGTGCACC
ATCTCAATTT
CTTTCATTTA
TACATCGTTT
TGCCTTCTTT
TATGTAACTA
TACTCCTCTA
AGTTTCAATC
TTGGCCATGT
AACCTCTGAT
CTATAGAATT
TTTTAAATGA
CTAGAATTAA
TGCCCATCTT
TTTTTTGGAC
CTAAATTCTT
CATGAAAATA
TATTACGAGG
GCTTATTCAG
AAGCTTTGGA
CTTCTTCGCC
AGAGGTTTGG
TCAAGTCTCC
AATCAAGGTT
GTCGGCTTGT
CTACCTTGCC
AGAAATTTAC
GAAAAGATGG
AAAAGGGTCA
AATCGTTGGT
AGATACGTTG
TTGACACTTC
TAAATAAGCG
AATTTCTTAT
GATTTATGAT
TTTTATTATT
AAATAAGTTA
TAAAAAAAAT
AAGTGTATAC
AAATTTTAAA
GTGACTCTTA
GGTTTTAAAA
CGAAAATTCT
TATTCTTGAG
TAACTCTTTC
CTGTAGGTCA
GGTTGCTTTC
TCAGGTATAG
CATGAGGTCG
CTCTTATTGA
CCACACCTCT
ACCGGCCGGT
CGAAATTCCC
CTACCCTATG
AACATATTCC
ATTTTGTAAT
TTCGTGTCGT
TTCTATTATG
AATTTCATTT
ATAAAGTTTA
TGTACAAATA
TCATAAAAAA
AGAGAATCTT
TTTAAGCAAG
GATTTTCTTA
ACTTCTTCGG
CGACAGCATC
ACCGACTTCG
GTGGTACTGT
TGGAACCACC
TAAATCACCA
GTTCTGATAC
CTGCATCCAA
AACCTTTTTA
ACTGCATCTT
CAATGGCCTT
ACCTTCTTCA
GGCAAGTTCA
ATGACAATTT
CAACATCATT
GCAGCAGACA
AGATAGTGGC
GATAGGGTTG
ACCTTATTCT
TTGGCAAATC
TGGAGCAGAA
CCGTGGCATG
GTTCGTACAA
ACCAAATGCG
GTGTTCTTGT
CTGGCAAAGA
GGCCAAGGAC
GCAGATGGCA
ACAAACCCAA
GGAACCTGGG
ATAACGGAGG
CTTCATCGGA
GATGATATCA
CCAAACATGT
TGCTGGTGAT
TATAATACCA
TTTAGGTGGG
TTGGGTTCTT
AACTAGGATC
ATGGCGGCAG
AATCAATCAA
TTGATGTTGA
ACCTTCAATG
TAGGAAATTC
GTTCTTGATG
GTTTCCTCCA
CAGTTTTTCT
CCATAATCTT
GAAGAGGCCA
AAACATTAGC
TTTATCCAAG
GACCAAATAG
GCAATGGTGG
CTCATGTTGT
AGGGCCATGA
AAGCGGCCAT
TCTTGTGATT
CTTTGCACTT
CTGGAACGGT
GTATTGTTCA
CTATCCCAAG
CGACACCATC
ACCATCGTCT
TCCTTTCTCT
TACCAAAGTA
AATACCTCCC
ACTAATTCTC
TGACAACAAC
GAAGTCAGTA
CCTTTAGCAA
ATTGTGGCTT
GATTGGAGAT
AAGTCTAAAA
GAGAGTCGGA
TGCAAAGTTA
CATGGTCTTA
AGTTGGCGTA
CAATTGAAGT
TCTTTACGGA
TTTTTAGTAA
ACCTTGTTCA
GGTCTAACAC
TACCTGTACC
CCATTTAGGA
CCACCCACAG
CACCTAACAA
AACGGCATCA
ACCTTCTTGG
AGGCTTCCAG
CGCCTCATCT
GGAAGTGGGA
CACCTGTAGC
GTCGATAGCA
GCACCACCAA
TTAAATGATT
TTCGAAATCG
AACTTGACAT
TGGAACGAAC
ATCAGAAATA
GCTTTAAGAA
CCTTAATGGC
TTCGGCTGTG
ATTTCTTGAC
CAACGTGGTC
ACCTGGCAAA
ACGACGATCT
TCTTAGGGGC
AGACATTAGA
ATGGTATATC
CTTGAAATAT
ATATATATAT
TGCTGAAATG
TAAAAGGTAA
GAAAAGTTAG
AAAGTAAGAC
GATTGCTAAC
CACCTATTGG
AAAAAACAAT
AGGTCCTTAA
ATAATATTGT
CAACTTCAAG
TATTGTGATG
CAAGCATTTA
GTCATGAACG
CTTCTCTATT
CTATATGAAA
AGCCGGTTCC
GGCGCTCTCA
CCTTTCCTTT
TTCTCCCAAT
TTTTCAGTTG
AAAAAGGTAT
ATGCGTCAGG
CGACCTCTGA
AATTAACAAA
AAATTTCCAG
TCATCGAATT
TGATTCTGTG
CGATAGCGCC
CCTGTGTGTT
CTCGTTATGT
TGAGGAAAAA
AATAATGGTT
GCTAAGAGAT
TCGAACTCTT
GCATCTTACG
ATACCTGAGT
ATTCCCACAG
TTGGGGATCT
CGACTCTAGC
TAGAGGATCA
ATTCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGATAACTTC
GTATAATGTA
TGCTATACGA
AGTTATTAGG
TCTGAAGAGG
AGTTTACGTC
CAGCCAAGCT
AGCTTGGCTG
CAGGTCGAGC
GGCCGCGATC
CGGAACCCTT
AATATAACTT
CGTATAATGT
ATGCTATACG
AAGTTATCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTCGC
GCGTTTCGGT
GATGACGGTG
AAAACCTCTG
ACACATGCAG
CTCCCGGAGA
CGGTCACAGC
TTGTCTGTAA
GCGGATGCCG
GGAGCAGACA
AGCCCGTCAG
GGCGCGTCAG
CGGGTGTTGG
CGGGTGTCGG
GGCTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TAACGCATTT
AAGCATAAAC
ACGCACTATG
CCGTTCTTCT
CATGTATATA
TATATACAGG
CAACACGCAG
ATATAGGTGC
GACGTGAACA
GTGAGCTGTA
TGTGCGCAGC
TCGCGTTGCA
TTTTCGGAAG
CGCTCGTTTT
CGGAAACGCT
TTGAAGTTCC
TATTCCGAAG
TTCCTATTCT
CTAGCTAGAA
AGTATAGGAA
CTTCAGAGCG
CTTTTGAAAA
CCAAAAGCGC
TCTGAAGACG
CACTTTCAAA
AAACCAAAAA
CGCACCGGAC
TGTAACGAGC
TACTAAAATA
TTGCGAATAC
CGCTTCCACA
AACATTGCTC
AAAAGTATCT
CTTTGCTATA
TATCTCTGTG
CTATATCCCT
ATATAACCTA
CCCATCCACC
TTTCGCTCCT
TGAACTTGCA
TCTAAACTCG
ACCTCTACAT
TTTTTATGTT
TATCTCTAGT
ATTACTCTTT
AGACAAAAAA
ATTGTAGTAA
GAACTATTCA
TAGAGTGAAT
CGAAAACAAT
ACGAAAATGT
AAACATTTCC
TATACGTAGT
ATATAGAGAC
AAAATAGAAG
AAACCGTTCA
TAATTTTCTG
ACCAATGAAG
AATCATCAAC
GCTATCACTT
TCTGTTCACA
AAGTATGCGC
AATCCACATC
GGTATAGAAT
ATAATCGGGG
ATGCCTTTAT
CTTGAAAAAA
TGCACCCGCA
GCTTCGCTAG
TAATCAGTAA
ACGCGGGAAG
TGGAGTCAGG
CTTTTTTTAT
GGAAGAGAAA
ATAGACACCA
AAGTAGCCTT
CTTCTAACCT
TAACGGACCT
ACAGTGCAAA
AAGTTATCAA
GAGACTGCAT
TATAGAGCGC
ACAAAGGAGA
AAAAAAGTAA
TCTAAGATGC
TTTGTTAGAA
AAATAGCGCT
CTCGGGATGC
ATTTTTGTAG
AACAAAAAAG
AAGTATAGAT
TCTTTGTTGG
TAAAATAGCG
CTCTCGCGTT
GCATTTCTGT
TCTGTAAAAA
TGCAGCTCAG
ATTCTTTGTT
TGAAAAATTA
GCGCTCTCGC
GTTGCATTTT
TGTTTTACAA
AAATGAAGCA
CAGATTCTTC
GTTGGTAAAA
TAGCGCTTTC
GCGTTGCATT
TCTGTTCTGT
AAAAATGCAG
CTCAGATTCT
TTGTTTGAAA
AATTAGCGCT
CTCGCGTTGC
ATTTTTGTTC
TACAAAATGA
AGCACAGATG
CTTCGTTGCT