名称: | pWPXL-EF1a-Cd80(mouse-opt-mouse)-Puro |
---|---|
别称: | NM_001359898.1;B71; Cd28l; Ly-53; Ly53; MIC17; TSA1 |
基因长: | 921bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pWPXL |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTGGAAGGGC
TAATTCACTC
CCAAAGAAGA
CAAGATATCC
TTGATCTGTG
GATCTACCAC
ACACAAGGCT
ACTTCCCTGA
TTAGCAGAAC
TACACACCAG
GGCCAGGGGT
CAGATATCCA
CTGACCTTTG
GATGGTGCTA
CAAGCTAGTA
CCAGTTGAGC
CAGATAAGGT
AGAAGAGGCC
AATAAAGGAG
AGAACACCAG
CTTGTTACAC
CCTGTGAGCC
TGCATGGGAT
GGATGACCCG
GAGAGAGAAG
TGTTAGAGTG
GAGGTTTGAC
AGCCGCCTAG
CATTTCATCA
CGTGGCCCGA
GAGCTGCATC
CGGAGTACTT
CAAGAACTGC
TGATATCGAG
CTTGCTACAA
GGGACTTTCC
GCTGGGGACT
TTCCAGGGAG
GCGTGGCCTG
GGCGGGACTG
GGGAGTGGCG
AGCCCTCAGA
TCCTGCATAT
AAGCAGCTGC
TTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
TCGACGGTAT
CGATGTCGAC
GATAAGCTTT
GCAAAGATGG
ATAAAGTTTT
AAACAGAGAG
GAATCTTTGC
AGCTAATGGA
CCTTCTAGGT
CTTGAAAGGA
GTGGGAATTG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GTAAGTGCCG
TGTGTGGTTC
CCGCGGGCCT
GGCCTCTTTA
CGGGTTATGG
CCCTTGCGTG
CCTTGAATTA
CTTCCACTGG
CTGCAGTACG
TGATTCTTGA
TCCCGAGCTT
CGGGTTGGAA
GTGGGTGGGA
GAGTTCGAGG
CCTTGCGCTT
AAGGAGCCCC
TTCGCCTCGT
GCTTGAGTTG
AGGCCTGGCC
TGGGCGCTGG
GGCCGCCGCG
TGCGAATCTG
GTGGCACCTT
CGCGCCTGTC
TCGCTGCTTT
CGATAAGTCT
CTAGCCATTT
AAAATTTTTG
ATGACCTGCT
GCGACGCTTT
TTTTCTGGCA
AGATAGTCTT
GTAAATGCGG
GCCAAGATCT
GCACACTGGT
ATTTCGGTTT
TTGGGGCCGC
GGGCGGCGAC
GGGGCCCGTG
CGTCCCAGCG
CACATGTTCG
GCGAGGCGGG
GCCTGCGAGC
GCGGCCACCG
AGAATCGGAC
GGGGGTAGTC
TCAAGCTGGC
CGGCCTGCTC
TGGTGCCTGG
CCTCGCGCCG
CCGTGTATCG
CCCCGCCCTG
GGCGGCAAGG
CTGGCCCGGT
CGGCACCAGT
TGCGTGAGCG
GAAAGATGGC
CGCTTCCCGG
CCCTGCTGCA
GGGAGCTCAA
AATGGAGGAC
GCGGCGCTCG
GGAGAGCGGG
CGGGTGAGTC
ACCCACACAA
AGGAAAAGGG
CCTTTCCGTC
CTCAGCCGTC
GCTTCATGTG
ACTCCACGGA
GTACCGGGCG
CCGTCCAGGC
ACCTCGATTA
GTTCTCGAGC
TTTTGGAGTA
CGTCGTCTTT
AGGTTGGGGG
GAGGGGTTTT
ATGCGATGGA
GTTTCCCCAC
ACTGAGTGGG
TGGAGACTGA
AGTTAGGCCA
GCTTGGCACT
TGATGTAATT
CTCCTTGGAA
TTTGCCCTTT
TTGAGTTTGG
ATCTTGGTTC
ATTCTCAAGC
CTCAGACAGT
GGTTCAAAGT
TTTTTTCTTC
CATTTCAGGT
GTCGTGAGGA
ATTTCGACAT
TTAAATTTAA
TTAATCTCGA
CGGTATCGGT
TAACTTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTATCGATCA
CGAGACTAGC
CTCGAGGTTT
AAACTACGGG
ATCCGCCACC
ATGGCCTGTA
ACTGTCAGTT
GATGCAAGAC
ACCCCGCTGC
TTAAGTTTCC
CTGTCCTAGG
CTGATTCTGC
TGTTTGTGTT
GTTGATACGC
CTGTCACAGG
TCTCAAGTGA
TGTCGACGAG
CAATTGTCTA
AAAGCGTCAA
GGACAAGGTC
TTGCTGCCGT
GCCGGTACAA
TTCACCTCAC
GAAGACGAAT
CCGAGGACCG
CATTTATTGG
CAGAAGCACG
ACAAAGTGGT
CCTGTCCGTG
ATAGCAGGAA
AACTGAAGGT
GTGGCCAGAG
TATAAGAACA
GGACCTTGTA
TGACAACACT
ACATATTCCT
TGATCATACT
CGGCCTGGTG
TTGAGCGACA
GGGGCACCTA
TTCTTGTGTG
GTACAGAAAA
AGGAGCGAGG
CACTTATGAG
GTTAAGCATC
TCGCCCTCGT
GAAGCTGAGT
ATAAAGGCAG
ACTTCAGTAC
ACCTAATATT
ACCGAGTCTG
GCAATCCTTC
TGCAGATACA
AAGAGGATCA
CCTGTTTCGC
TAGCGGAGGA
TTTCCGAAGC
CCAGATTTAG
TTGGTTGGAA
AATGGGCGGG
AACTTCCGGG
CATTAATACA
ACCATTAGCC
AGGATCCAGA
GTCAGAACTT
TACACTATCT
CATCCCAGCT
GGACTTTAAT
ACGACCCGGA
ACCACACTAT
AAAGTGCTTG
ATCAAATACG
GGGACGCCCA
CGTTAGTGAG
GATTTCACTT
GGGAGAAGCC
CCCAGAAGAT
CCTCCTGACA
GTAAAAACAC
ATTGGTACTT
TTCGGGGCTG
GCTTCGGAGC
AGTTATTACA
GTGGTTGTGA
TAGTCGTCAT
AATCAAGTGT
TTCTGCAAAC
ATCGCAGTTG
TTTTCGCCGC
AACGAAGCTA
GCCGGGAGAC
AAATAATAGC
CTTACCTTTG
GCCCAGAAGA
GGCACTGGCA
GAGCAGACAG
TCTTCCTGTA
GGAATTCAAT
TCTACCGGGT
AGGGGAGGCG
CTTTTCCCAA
GGCAGTCTGG
AGCATGCGCT
TTAGCAGCCC
CGCTGGGCAC
TTGGCGCTAC
ACAAGTGGCC
TCTGGCCTCG
CACACATTCC
ACATCCACCG
GTAGGCGCCA
ACCGGCTCCG
TTCTTTGGTG
GCCCCTTCGC
GCCACCTTCT
ACTCCTCCCC
TAGTCAGGAA
GTTCCCCCCC
GCCCCGCAGC
TCGCGTCGTG
CAGGACGTGA
CAAATGGAAG
TAGCACGTCT
CACTAGTCTC
GTGCAGATGG
ACAGCACCGC
TGAGCAATGG
AAGCGGGTAG
GCCTTTGGGG
CAGCGGCCAA
TAGCAGCTTT
GCTCCTTCGC
TTTCTGGGCT
CAGAGGCTGG
GAAGGGGTGG
GTCCGGGGGC
GGGCTCAGGG
GCGGGCTCAG
GGGCGGGGCG
GGCGCCCGAA
GGTCCTCCGG
AGGCCCGGCA
TTCTGCACGC
TTCAAAAGCG
CACGTCTGCC
GCGCTGTTCT
CCTCTTCCTC
ATCTCCGGGC
CTTTCGACCT
GCAGCCCAAG
CTTACCATGA
CCGAGTACAA
GCCCACGGTG
CGCCTCGCCA
CCCGCGACGA
CGTCCCCAGG
GCCGTACGCA
CCCTCGCCGC
CGCGTTCGCC
GACTACCCCG
CCACGCGCCA
CACCGTCGAT
CCGGACCGCC
ACATCGAGCG
GGTCACCGAG
CTGCAAGAAC
TCTTCCTCAC
GCGCGTCGGG
CTCGACATCG
GCAAGGTGTG
GGTCGCGGAC
GACGGCGCCG
CGGTGGCGGT
CTGGACCACG
CCGGAGAGCG
TCGAAGCGGG
GGCGGTGTTC
GCCGAGATCG
GCCCGCGCAT
GGCCGAGTTG
AGCGGTTCCC
GGCTGGCCGC
GCAGCAACAG
ATGGAAGGCC
TCCTGGCGCC
GCACCGGCCC
AAGGAGCCCG
CGTGGTTCCT
GGCCACCGTC
GGCGTCTCGC
CCGACCACCA
GGGCAAGGGT
CTGGGCAGCG
CCGTCGTGCT
CCCCGGAGTG
GAGGCGGCCG
AGCGCGCCGG
GGTGCCCGCC
TTCCTGGAGA
CCTCCGCGCC
CCGCAACCTC
CCCTTCTACG
AGCGGCTCGG
CTTCACCGTC
ACCGCCGACG
TCGAGGTGCC
CGAAGGACCG
CGCACCTGGT
GCATGACCCG
CAAGCCCGGT
GCCTGAACTA
GTCATATGAT
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAGCTGA
CGTCCTTTCC
ATGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCA
TCGGTACGTA
TGGCCAGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATGGGATCAA
TTCACCATGG
GAATAACTTC
GTATAGCATA
CATTATACGA
AGTTATGCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGCAT
CTAGAATTAA
TTCCGTGTAT
TCTATAGTGT
CACCTAAATC
GTATGTGTAT
GATACATAAG
GTTATGTATT
AATTGTAGCC
GCGTTCTAAC
GACAATATGT
ACAAGCCTAA
TTGTGTAGCA
TCTGGCTTAC
TGAAGCAGAC
CCTATCATCT
CTCTCGTAAA
CTGCCGTCAG
AGTCGGTTTG
GTTGGACGAA
CCTTCTGAGT
TTCTGGTAAC
GCCGTCCCGC
ACCCGGAAAT
GGTCAGCGAA
CCAATCAGCA
GGGTCATCGC
TAGCCAGATC
CTCTACGCCG
GACGCATCGT
GGCCGGCATC
ACCGGCGCCA
CAGGTGCGGT
TGCTGGCGCC
TATATCGCCG
ACATCACCGA
TGGGGAAGAT
CGGGCTCGCC
ACTTCGGGCT
CATGAGCGCT
TGTTTCGGCG
TGGGTATGGT
GGCAGGCCCC
GTGGCCGGGG
GACTGTTGGG
CGCCATCTCC
TTGCATGCAC
CATTCCTTGC
GGCGGCGGTG
CTCAACGGCC
TCAACCTACT
ACTGGGCTGC
TTCCTAATGC
AGGAGTCGCA
TAAGGGAGAG
CGTCGAATGG
TGCACTCTCA
GTACAATCTG
CTCTGATGCC
GCATAGTTAA
GCCAGCCCCG
ACACCCGCCA
ACACCCGCTG
ACGCGCCCTG
ACGGGCTTGT
CTGCTCCCGG
CATCCGCTTA
CAGACAAGCT
GTGACCGTCT
CCGGGAGCTG
CATGTGTCAG
AGGTTTTCAC
CGTCATCACC
GAAACGCGCG
AGACGAAAGG
GCCTCGTGAT
ACGCCTATTT
TTATAGGTTA
ATGTCATGAT
AATAATGGTT
TCTTAGACGT
CAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ATGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTATTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTC
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGGCGCAGC
GGTCGGGCTG
AACGGGGGGT
TCGTGCACAC
AGCCCAGCTT
GGAGCGAACG
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCATTGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
AGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TTGGACACAA
GACAGGCTTG
CGAGATATGT
TTGAGAATAC
CACTTTATCC
CGCGTCAGGG
AGAGGCAGTG
CGTAAAAAGA
CGCGGACTCA
TGTGAAATAC
TGGTTTTTAG
TGCGCCAGAT
CTCTATAATC
TCGCGCAACC
TATTTTCCCC
TCGAACACTT
TTTAAGCCGT
AGATAAACAG
GCTGGGACAC
TTCACATGAG
CGAAAAATAC
ATCGTCACCT
GGGACATGTT
GCAGATCCAT
GCACGTAAAC
TCGCAAGCCG
ACTGATGCCT
TCTGAACAAT
GGAAAGGCAT
TATTGCCGTA
AGCCGTGGCG
GTCTGTACCG
GGTGCGTTAC
TGGCGCGTGA
ACTGGGTATT
CGTCATGTCG
ATACCGTTTG
TATTTCCAGC
TACGATCACG
ACAACCAGCG
CGAGCTTAAA
GTGCTGAAAC
GCGCAGAAGG
CGATGGCGAA
GGCTTCATCG
TTATTGATGA
CCTGGTGGAT
ACCGGTGGTA
CTGCGGTTGC
GATTCGTGAA
ATGTATCCAA
AAGCGCACTT
TGTCACCATC
TTCGCAAAAC
CGGCTGGTCG
TCCGCTGGTT
GATGACTATG
TTGTTGATAT
CCCGCAAGAT
ACCTGGATTG
AACAGCCGTG
GGATATGGGC
GTCGTATTCG
TCCCGCCAAT
CTCCGGTCGC
TAATCTTTTC
AACGCCTGGC
ACTGCCGGGC
GTTGTTCTTT
TTAACTTCAG
GCGGGTTACA
ATAGTTTCCA
GTAAGTATTC
TGGAGGCTGC
ATCCATGACA
CAGGCAAACC
TGAGCGAAAC
CCTGTTCAAA
CCCCGCTTTA
AACATCCTGA
AACCTCGACG
CTAGTCCGCC
GCTTTAATCA
CGGCGCACAA
CCGCCTGTGC
AGTCGGCCCT
TGATGGTAAA
ACCATCCCTC
ACTGGTATCG
CATGATTAAC
CGTCTGATGT
GGATCTGGCG
CGGCATTGAC
CCACGCGAAA
TCCTCGACGT
CCAGGCACGT
ATTGTGATGA
GCGATGCCGA
ACGTACCGAC
GATGATTTAT
ACGATACGGT
GATTGGCTAC
CGTGGCGGCA
ACTGGATTTA
TGAGTGGGCC
CCGGATCTTT
GTGAAGGAAC
CTTACTTCTG
TGGTGTGACA
TAATTGGACA
AACTACCTAC
AGAGATTTAA
AGCTCTAAGG
TAAATATAAA
ATTTTTAAGT
GTATAATGTG
TTAAACTACT
GATTCTAATT
GTTTGTGTAT
TTTAGATTCC
AACCTATGGA
ACTGATGAAT
GGGAGCAGTG
GTGGAATGCC
TTTAATGAGG
AAAACCTGTT
TTGCTCAGAA
GAAATGCCAT
CTAGTGATGA
TGAGGCTACT
GCTGACTCTC
AACATTCTAC
TCCTCCAAAA
AAGAAGAGAA
AGGTAGAAGA
CCCCAAGGAC
TTTCCTTCAG
AATTGCTAAG
TTTTTTGAGT
CATGCTGTGT
TTAGTAATAG
AACTCTTGCT
TGCTTTGCTA
TTTACACCAC
AAAGGAAAAA
GCTGCACTGC
TATACAAGAA
AATTATGGAA
AAATATTCTG
TAACCTTTAT
AAGTAGGCAT
AACAGTTATA
ATCATAACAT
ACTGTTTTTT
CTTACTCCAC
ACAGGCATAG
AGTGTCTGCT
ATTAATAACT
ATGCTCAAAA
ATTGTGTACC
TTTAGCTTTT
TAATTTGTAA
AGGGGTTAAT
AAGGAATATT
TGATGTATAG
TGCCTTGACT
AGAGATCATA
ATCAGCCATA
CCACATTTGT
AGAGGTTTTA
CTTGCTTTAA
AAAACCTCCC
ACACCTCCCC
CTGAACCTGA
AACATAAAAT
GAATGCAATT
GTTGTTGTTA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GATCAACTGG
ATAACTCAAG
CTAACCAAAA
TCATCCCAAA
CTTCCCACCC
CATACCCTAT
TACCACTGCC
AATTACCTAG
TGGTTTCATT
TACTCTAAAC
CTGTGATTCC
TCTGAATTAT
TTTCATTTTA
AAGAAATTGT
ATTTGTTAAA
TATGTACTAC
AAACTTAGTA
G