名称: | pCMV-IARS1(human)-Neo |
---|---|
别称: | NM_013417.4;GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785 |
基因长: | 3786bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCDNA3.1(+) |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGTATCTG
CTCCCTGCTT
GTGTGTTGGA
GGTCGCTGAG
TAGTGCGCGA
GCAAAATTTA
AGCTACAACA
AGGCAAGGCT
TGACCGACAA
TTGCATGAAG
AATCTGCTTA
GGGTTAGGCG
TTTTGCGCTG
CTTCGCGATG
TACGGGCCAG
ATATACGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTCTCTG
GCTAACTAGA
GAACCCACTG
CTTACTGGCT
TATCGAAATT
AATACGACTC
ACTATAGGGA
GACCCAAGCT
GGCTAGCCGC
CACCATGCTT
CAACAAGTTC
CAGAAAACAT
AAATTTTCCT
GCTGAAGAAG
AGAAAATCTT
GGAGTTTTGG
ACTGAATTTA
ATTGTTTTCA
GGAATGCTTA
AAGCAATCAA
AACATAAACC
AAAATTTACC
TTCTATGATG
GTCCTCCTTT
TGCAACTGGA
CTGCCTCACT
ATGGACATAT
ACTTGCGGGT
ACAATTAAAG
ATATAGTTAC
AAGATATGCT
CACCAGAGTG
GGTTTCATGT
TGACAGAAGA
TTTGGATGGG
ATTGCCATGG
CTTACCTGTG
GAATATGAAA
TTGATAAGAC
ACTGGGAATC
AGAGGACCAG
AGGATGTGGC
CAAAATGGGG
ATTACAGAGT
ATAACAATCA
GTGCCGAGCA
ATTGTGATGA
GATATTCTGC
TGAGTGGAAG
TCTACTGTTA
GCAGACTTGG
CCGATGGATT
GACTTTGACA
ATGACTATAA
AACTCTGTAT
CCACAATTCA
TGGAATCAGT
CTGGTGGGTC
TTCAAACAAC
TCTATGATAA
AGGCCTTGTT
TATAGAGGTG
TGAAAGTCAT
GCCCTTCTCT
ACGGCATGTA
ACACTCCACT
TTCCAACTTC
GAGTCACACC
AGAATTATAA
GGATGTTCAA
GATCCTTCAG
TATTTGTAAC
TTTCCCTTTG
GAAGAAGATG
AAACTGTATC
TTTAGTTGCT
TGGACAACCA
CTCCCTGGAC
TCTACCTAGT
AACCTTGCTG
TGTGTGTTAA
TCCAGAAATG
CAATATGTGA
AAATTAAAGA
TGTTGCCAGA
GGACGATTAC
TCATTTTAAT
GGAAGCCAGA
TTGTCAGCCC
TCTATAAATT
GGAGAGTGAC
TATGAGATCC
TTGAAAGATT
TCCTGGTGCC
TATCTTAAAG
GCAAGAAGTA
CAGGCCCCTG
TTTGACTATT
TCCTGAAGTG
TAAAGAGAAT
GGCGCTTTCA
CTGTGCTTGT
TGACAACTAT
GTGAAGGAAG
AAGAAGGCAC
AGGGGTTGTC
CACCAAGCTC
CTTACTTCGG
TGCTGAGGAC
TATCGGGTCT
GTATGGACTT
TAACATTATT
CGGAAAGACT
CACTCCCTGT
TTGCCCTGTG
GATGCTTCAG
GCTGCTTCAC
AACGGAGGTG
ACAGATTTCG
CAGGACAGTA
TGTGAAGGAT
GCTGACAAAA
GTATCATCAG
GACTTTGAAG
GAACAAGGCC
GACTTCTGGT
TGCCACCACC
TTCACTCACA
GCTACCCTTT
TTGCTGGAGA
TCAGACACTC
CTCTAATTTA
CAAAGCAGTG
CCCAGCTGGT
TTGTGCGAGT
GGAGAACATG
GTGGACCAGC
TCCTAAGGAA
CAATGACCTG
TGCTACTGGG
TCCCAGAGTT
GGTACGAGAA
AAACGATTTG
GAAATTGGCT
GAAAGATGCA
CGTGACTGGA
CAATTTCCAG
AAACAGATAC
TGGGGCACCC
CCATCCCACT
GTGGGTCAGC
GATGACTTTG
AGGAGGTGGT
ATGCATTGGG
TCAGTGGCGG
AACTTGAAGA
ACTGTCAGGA
GCAAAGATCT
CAGATCTCCA
CAGAGAGAGT
GTTGACCACC
TGACCATTCC
TTCACGCTGT
GGGAAGGGAT
CCTTGCACCG
CATCTCTGAA
GTGTTTGACT
GTTGGTTTGA
GAGTGGCAGC
ATGCCCTATG
CTCAGGTTCA
TTACCCGTTT
GAAAACAAGA
GGGAGTTTGA
GGATGCTTTT
CCTGCAGATT
TCATTGCCGA
GGGCATCGAC
CAAACCAGAG
GATGGTTTTA
TACCCTGCTG
GTGCTGGCCA
CGGCCCTCTT
TGGACAACCG
CCTTTCAAGA
ACGTAATTGT
GAATGGGCTT
GTCCTGGCAA
GTGATGGCCA
AAAAATGAGC
AAACGGAAAA
AGAATTATCC
AGATCCAGTT
TCCATCATCC
AGAAGTATGG
TGCTGATGCC
CTCAGATTAT
ATCTGATTAA
CTCCCCTGTG
GTGAGAGCAG
AAAACCTCCG
CTTTAAAGAA
GAGGGTGTGC
GGGACGTCCT
TAAGGATGTA
CTGCTCCCAT
GGTACAATGC
CTATCGCTTC
TTAATCCAGA
ACGTTCTGAG
GCTCCAGAAG
GAGGAAGAAA
TAGAATTTCT
CTACAATGAG
AACACGGTTA
GAGAAAGCCC
CAACATTACA
GACCGGTGGA
TCCTGTCCTT
CATGCAGTCT
CTCATTGGCT
TCTTTGAGAC
TGAAATGGCA
GCTTATAGGC
TTTATACTGT
GGTGCCTCGC
CTGGTCAAGT
TTGTAGATAT
TCTGACCAAT
TGGTATGTTA
GAATGAACCG
CAGAAGATTA
AAGGGTGAAA
ATGGGATGGA
GGATTGTGTC
ATGGCCCTAG
AAACCTTGTT
TAGTGTTCTG
CTTTCTCTTT
GCAGACTTAT
GGCTCCCTAC
ACACCTTTTC
TCACTGAATT
GATGTACCAG
AATCTAAAGG
TGCTGATTGA
CCCTGTTTCT
GTTCAGGACA
AGGACACACT
CAGCATTCAC
TACCTCATGC
TGCCCCGTGT
TCGAGAAGAA
TTGATTGACA
AGAAAACAGA
GAGTGCAGTA
TCTCAGATGC
AGTCTGTGAT
TGAACTTGGA
AGAGTGATCA
GAGACCGAAA
AACTATTCCC
ATAAAGTATC
CTTTGAAAGA
AATTGTGGTT
ATCCATCAAG
ATCCAGAAGC
TCTTAAAGAT
ATCAAGTCTT
TGGAGAAGTA
TATCATTGAG
GAACTCAATG
TTCGAAAAGT
TACACTGTCT
ACAGATAAAA
ACAAGTATGG
CATTCGGCTA
AGGGCAGAAC
CAGATCACAT
GGTCCTGGGG
AAGCGTCTGA
AGGGAGCCTT
TAAGGCAGTG
ATGACGTCCA
TCAAGCAGTT
GAGCAGTGAG
GAGCTGGAGC
AGTTCCAGAA
GACTGGGACC
ATTGTTGTGG
AAGGCCATGA
ATTGCACGAT
GAAGACATCC
GCCTCATGTA
CACCTTTGAT
CAGGCCACAG
GTGGGACTGC
GCAATTTGAA
GCACACTCAG
ATGCTCAGGC
TTTGGTCCTC
TTAGATGTCA
CTCCTGACCA
GTCAATGGTA
GATGAAGGAA
TGGCTCGGGA
AGTCATCAAT
CGCATACAGA
AACTTCGCAA
AAAGTGCAAT
CTGGTTCCAA
CTGATGAAAT
CACAGTGTAC
TATAAAGCAA
AGTCTGAAGG
AACATATCTG
AATAGTGTTA
TTGAAAGCCA
CACAGAGTTC
ATATTTACCA
CCATAAAGGC
TCCCTTGAAA
CCATATCCAG
TTTCTCCATC
GGATAAAGTC
CTTATTCAAG
AAAAAACACA
GTTGAAGGGA
TCTGAACTGG
AAATTACACT
CACCAGAGGA
TCTTCCCTTC
CTGGTCCTGC
TTGTGCATAT
GTCAATCTTA
ACATTTGTGC
AAATGGCAGT
GAACAAGGTG
GAGTATTGCT
CCTGGAAAAT
CCAAAAGGTG
ACAATAGGTT
GGACCTTTTA
AAGCTGAAGA
GTGTTGTCAC
TAGCATTTTT
GGTGTGAAAA
ATACAGAGCT
GGCTGTCTTC
CATGATGAAA
CAGAAATACA
AAACCAAACT
GACTTACTGA
GTCTTAGTGG
AAAAACACTT
TGTGTGACTG
CAGGATCGGC
TCCCTCTCTG
ATCAACAGTT
CTAGTACTCT
TCTTTGTCAG
TATATCAACC
TACAGCTCCT
GAATGCAAAG
CCACAAGAGT
GTTTAATGGG
GACAGTGGGC
ACTCTCCTGC
TTGAAAACCC
ACTTGGGCAG
AATGGACTCA
CCCACCAAGG
TCTTCTGTAT
GAAGCAGCCA
AGGTGTTTGG
CCTTCGGAGC
AGGAAGCTAA
AGCTGTTTCT
GAATGAGACC
CAAACGCAGG
AAATTACAGA
AGACATCCCC
GTGAAGACTT
TGAATATGAA
GACTGTGTAT
GTTTCTGTGT
TACCAACAAC
AGCAGACTTC
TAGGCGGCCG
CTCGAGTCTA
GAGGGCCCGT
TTAAACCCGC
TGATCAGCCT
CGACTGTGCC
TTCTAGTTGC
CAGCCATCTG
TTGTTTGCCC
CTCCCCCGTG
CCTTCCTTGA
CCCTGGAAGG
TGCCACTCCC
ACTGTCCTTT
CCTAATAAAA
TGAGGAAATT
GCATCGCATT
GTCTGAGTAG
GTGTCATTCT
ATTCTGGGGG
GTGGGGTGGG
GCAGGACAGC
AAGGGGGAGG
ATTGGGAAGA
CAATAGCAGG
CATGCTGGGG
ATGCGGTGGG
CTCTATGGCT
TCTGAGGCGG
AAAGAACCAG
CTGGGGCTCT
AGGGGGTATC
CCCACGCGCC
CTGTAGCGGC
GCATTAAGCG
CGGCGGGTGT
GGTGGTTACG
CGCAGCGTGA
CCGCTACACT
TGCCAGCGCC
CTAGCGCCCG
CTCCTTTCGC
TTTCTTCCCT
TCCTTTCTCG
CCACGTTCGC
CGGCTTTCCC
CGTCAAGCTC
TAAATCGGGG
GCTCCCTTTA
GGGTTCCGAT
TTAGTGCTTT
ACGGCACCTC
GACCCCAAAA
AACTTGATTA
GGGTGATGGT
TCACGTAGTG
GGCCATCGCC
CTGATAGACG
GTTTTTCGCC
CTTTGACGTT
GGAGTCCACG
TTCTTTAATA
GTGGACTCTT
GTTCCAAACT
GGAACAACAC
TCAACCCTAT
CTCGGTCTAT
TCTTTTGATT
TATAAGGGAT
TTTGCCGATT
TCGGCCTATT
GGTTAAAAAA
TGAGCTGATT
TAACAAAAAT
TTAACGCGAA
TTAATTCTGT
GGAATGTGTG
TCAGTTAGGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCTGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
CCCGGGAGCT
TGTATATCCA
TTTTCGGATC
TGATCAAGAG
ACAGGATGAG
GATCGTTTCG
CATGATTGAA
CAAGATGGAT
TGCACGCAGG
TTCTCCGGCC
GCTTGGGTGG
AGAGGCTATT
CGGCTATGAC
TGGGCACAAC
AGACAATCGG
CTGCTCTGAT
GCCGCCGTGT
TCCGGCTGTC
AGCGCAGGGG
CGCCCGGTTC
TTTTTGTCAA
GACCGACCTG
TCCGGTGCCC
TGAATGAACT
GCAGGACGAG
GCAGCGCGGC
TATCGTGGCT
GGCCACGACG
GGCGTTCCTT
GCGCAGCTGT
GCTCGACGTT
GTCACTGAAG
CGGGAAGGGA
CTGGCTGCTA
TTGGGCGAAG
TGCCGGGGCA
GGATCTCCTG
TCATCTCACC
TTGCTCCTGC
CGAGAAAGTA
TCCATCATGG
CTGATGCAAT
GCGGCGGCTG
CATACGCTTG
ATCCGGCTAC
CTGCCCATTC
GACCACCAAG
CGAAACATCG
CATCGAGCGA
GCACGTACTC
GGATGGAAGC
CGGTCTTGTC
GATCAGGATG
ATCTGGACGA
AGAGCATCAG
GGGCTCGCGC
CAGCCGAACT
GTTCGCCAGG
CTCAAGGCGC
GCATGCCCGA
CGGCGAGGAT
CTCGTCGTGA
CCCATGGCGA
TGCCTGCTTG
CCGAATATCA
TGGTGGAAAA
TGGCCGCTTT
TCTGGATTCA
TCGACTGTGG
CCGGCTGGGT
GTGGCGGACC
GCTATCAGGA
CATAGCGTTG
GCTACCCGTG
ATATTGCTGA
AGAGCTTGGC
GGCGAATGGG
CTGACCGCTT
CCTCGTGCTT
TACGGTATCG
CCGCTCCCGA
TTCGCAGCGC
ATCGCCTTCT
ATCGCCTTCT
TGACGAGTTC
TTCTGAGCGG
GACTCTGGGG
TTCGAAATGA
CCGACCAAGC
GACGCCCAAC
CTGCCATCAC
GAGATTTCGA
TTCCACCGCC
GCCTTCTATG
AAAGGTTGGG
CTTCGGAATC
GTTTTCCGGG
ACGCCGGCTG
GATGATCCTC
CAGCGCGGGG
ATCTCATGCT
GGAGTTCTTC
GCCCACCCCA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
TATACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
TCCCGATCCC
CTATGGT