名称: | pLV-Tet-miniCMV-HECTD2(human)-GFP-nanobody-mCherry-Puro |
---|---|
别称: | NM_001284274.3; |
基因长: | 2343bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 红色 |
启动子: | Tet-miniCMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV-Tet-miniCMV-MCS-GFP-nanobody-mCherry-Puro |
正向引物: | PFLAG-CMV-F |
反向引物: | |
诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTCGATCC
GCAGCAGTGA
ACTCCAAGCC
ACCATGAGTG
AGGCGGTTCG
GGTACCCTCG
CCCGCCACTC
CGCTGGTGGT
GGCGGCGCCC
GCGCCTGAGG
AGAGGAAAGG
GAAGGAGTCA
GAGCGCGAGA
AGCTGCCGCC
CATCGTATCG
GCGGGCGCCG
GCGCGACCGC
GGGTTTGGAC
AGAGGAGCCA
AAGGCCAAAT
TTCCACTTTC
AGCAGTTTTA
TTTCAGCTGT
TAGCCCGAAG
AAAGAAGCTG
CTGAAAACAG
AAGTTCACCT
GCACATCTTG
TTTTCCCTAA
CATCAAGAAT
GTGAGAGAAC
CACCACCAAT
TTGCCTTGAT
GTTAGACAAA
AACAGCGTAC
ATCTATGGAT
GCATCATCAT
CCGAAATGAA
GGCCCCAGTC
CTTCCAGAAC
CTATTCTTCC
TATCCAGCCC
AAAACTGTGA
AAGACTTTCA
GGAAGATGTA
GAAAAAGTTA
AGTCATCAGG
AGATTGGAAA
GCAGTACATG
ATTTTTATCT
AACAACGTTT
GATTCTTTCC
CAGAATTAAA
TGCTGCATTT
AAGAAAGATG
CCACTGCCTC
ATTTAACACC
ATTGAAGACT
CTGGGATTAA
TGCTAAATTT
GTGAATGCTG
TGTATGATAC
CTTACTTAAT
ACTCCTCAAG
ACGTTCAGAA
GACAGTATTA
AAGGGAATCA
TTAACAGCCT
GTTACGAGAA
TGGAAAGGTC
CACGAACAAA
AGATGATCTT
AGAGCATATT
TTATACTTTT
ACAGAATCCT
CAGTTTAATA
ACACATCTAC
GTATGTCATC
TATGCTCACT
TGCTACGACA
GATAGCTACC
TTAGTGGAAG
CTGACCATCA
TTTCCTAGTT
CACTGGTTTA
AAAAACAGTA
TTCAAGATTG
TCCCAGAAGA
GGTTCAAACA
ATTGGTAGAG
AGATTGCTGC
AATTTATTTC
TTTACGCCTG
TTTCCTGCAA
AGCCTGAAGA
ATTTCCACCT
ATAACAAAGT
GTTCCTGGTG
GATTCCATCA
GCCGCTAAAG
TGTTGGCTTT
ACTTAATACT
GCAAACAATT
TAGTTCACCC
TCCCCTTATT
CCTTATACTG
ATTTCTATAA
TTCTACATTG
GATCACATTG
ATCTCATGGA
AGAATATCAT
ACTTGGCAAA
ACTTTGGAAA
CTCTCACAGG
TTTTCCTTCT
GTCAGTACCC
ATTCGTTATT
TCTGTAGCTG
CAAAAAAAAT
CATTATTCAG
AGAGACTCAG
AGCAACAGAT
GATAAACATC
GCAAGGCAAA
GTCTGGTGGA
TAAAGTATCT
CGAAGACAGA
GACCTGATAT
GAATATATTA
TTTCTAAATA
TGAAAGTAAG
ACGGACACAT
CTGGTTAGCG
ATTCACTTGA
TGAGCTAACC
CGGAAAAGAG
CCGATTTGAA
AAAGAAGTTA
AAAGTTACAT
TTGTAGGGGA
AGCTGGTTTG
GATATGGGTG
GTTTGACTAA
AGAATGGTTC
CTTCTTCTAA
TTCGCCAAAT
TTTTCATCCA
GATTATGGCA
TGTTTACATA
TCACAAGGAT
TCACACTGCC
ATTGGTTTAG
CAGCTTTAAA
TGTGATAACT
ATTCTGAATT
CCGATTGGTT
GGAATTCTTA
TGGGACTAGC
TGTTTATAAC
AGCATCACCT
TGGATATTCG
TTTCCCTCCC
TGCTGTTACA
AGAAATTATT
GAGCCCTCCC
ATCATTCCTA
GTGATCAAAA
TATACCAGTA
GGCATCTGCA
ATGTTACCGT
GGACGACTTA
TGTCAAATTA
TGCCTGAGTT
GGCCCATGGA
TTAAGTGAAC
TCTTATCACA
TGAAGGCAAT
GTTGAAGAAG
ATTTCTATTC
AACATTTCAG
GTTTTTCAAG
AAGAATTTGG
AATAATCAAG
TCCTATAATT
TAAAGCCCGG
TGGTGATAAA
ATTTCAGTTA
CCAATCAAAA
TAGAAAAGAA
TATGTACAGC
TTTATACTGA
CTTCCTTCTG
AACAAATCCA
TCTATAAGCA
GTTTGCTGCA
TTTTATTATG
GATTTCATAG
TGTGTGTGCT
TCAAATGCCC
TAATGCTGCT
TCGTCCAGAA
GAGGTTGAAA
TTTTGGTCTG
TGGAAGTCCT
GACCTGGATA
TGCATGCTCT
GCAGAGAAGT
ACTCAGTATG
ATGGCTATGC
AAAAACGGAC
TTAACTATAA
AATACTTTTG
GGATGTTGTG
CTTGGATTTC
CTCTTGATCT
TCAAAAGAAG
TTGCTACATT
TTACTACAGG
GAGTGACAGA
GTACCTGTAG
GAGGGATGGC
TGATTTGAAC
TTTAAAATCT
CAAAGAATGA
AACTTCTACT
AACTGCTTAC
CTGTGGCCCA
TACCTGCTTC
AATCAACTTT
GCCTTCCCCC
CTACAAGAGC
AAAAAGGATC
TGAAACAGAA
ATTGATCATT
GGAATTTCAA
ATTCAGAAGG
TTTTGGACTT
GAGTACCCCT
ACGACGTGCC
TGACTACGCC
GGCGGCGGTG
GTTCAGGCGG
TGGCGGTTCC
GGTGGTGGGG
GATCAGTCCA
ACTGGTGGAG
TCTGGTGGCG
CTTTGGTGCA
GCCAGGTGGC
TCTCTGCGTT
TGTCCTGTGC
CGCTTCTGGC
TTCCCAGTGA
ACCGCTATTC
CATGCGCTGG
TATCGCCAGG
CTCCAGGCAA
AGAGCGTGAG
TGGGTAGCCG
GTATGTCCAG
CGCGGGTGAT
CGTAGCTCCT
ATGAAGACTC
CGTGAAGGGC
CGTTTCACCA
TCAGCCGTGA
CGATGCCCGT
AACACGGTGT
ATCTGCAAAT
GAACAGCTTG
AAACCTGAAG
ATACGGCCGT
GTATTACTGT
AATGTGAACG
TGGGCTTCGA
GTATTGGGGC
CAAGGCACCC
AGGTCACCGT
CTCCAGCTAA
GATCCTTGAC
TTGCGGCCGC
AACTCCCACC
TGCAACATGC
GTGACTGACT
GAGGCCGCGA
CTCTAGAGTC
GACCTGCATC
TAGGCGCCGG
AATTAGATCT
CTCGAGGTTA
ACGAATTCTA
CCGGGTAGGG
GAGGCGCTTT
TCCCAAGGCA
GTCTGGAGCA
TGCGCTTTAG
CAGCCCCGCT
GGGCACTTGG
CGCTACACAA
GTGGCCTCTG
GCCTCGCACA
CATTCCACAT
CCACCGGTAG
GCGCCAACCG
GCTCCGTTCT
TTGGTGGCCC
CTTCGCGCCA
CCTTCTACTC
CTCCCCTAGT
CAGGAAGTTC
CCCCCCGCCC
CGCAGCTCGC
GTCGTGCAGG
ACGTGACAAA
TGGAAGTAGC
ACGTCTCACT
AGTCTCGTGC
AGATGGACAG
CACCGCTGAG
CAATGGAAGC
GGGTAGGCCT
TTGGGGCAGC
GGCCAATAGC
AGCTTTGCTC
CTTCGCTTTC
TGGGCTCAGA
GGCTGGGAAG
GGGTGGGTCC
GGGGGCGGGC
TCAGGGGCGG
GCTCAGGGGC
GGGGCGGGCG
CCCGAAGGTC
CTCCGGAGGC
CCGGCATTCT
GCACGCTTCA
AAAGCGCACG
TCTGCCGCGC
TGTTCTCCTC
TTCCTCATCT
CCGGGCCTTT
CGACCTGCAG
CCCAAGCTTA
CCATGGTGAG
CAAGGGCGAG
GAGGATAACA
TGGCCATCAT
CAAGGAGTTC
ATGCGCTTCA
AGGTGCACAT
GGAGGGCTCC
GTGAACGGCC
ACGAGTTCGA
GATCGAGGGC
GAGGGCGAGG
GCCGCCCCTA
CGAGGGCACC
CAGACCGCCA
AGCTGAAGGT
GACCAAGGGT
GGCCCCCTGC
CCTTCGCCTG
GGACATCCTG
TCCCCTCAGT
TCATGTACGG
CTCCAAGGCC
TACGTGAAGC
ACCCCGCCGA
CATCCCCGAC
TACTTGAAGC
TGTCCTTCCC
CGAGGGCTTC
AAGTGGGAGC
GCGTGATGAA
CTTCGAGGAC
GGCGGCGTGG
TGACCGTGAC
CCAGGACTCC
TCCCTGCAGG
ACGGCGAGTT
CATCTACAAG
GTGAAGCTGC
GCGGCACCAA
CTTCCCCTCC
GACGGCCCCG
TAATGCAGAA
GAAGACCATG
GGCTGGGAGG
CCTCCTCCGA
GCGGATGTAC
CCCGAGGACG
GCGCCCTGAA
GGGCGAGATC
AAGCAGAGGC
TGAAGCTGAA
GGACGGCGGC
CACTACGACG
CTGAGGTCAA
GACCACCTAC
AAGGCCAAGA
AGCCCGTGCA
GCTGCCCGGC
GCCTACAACG
TCAACATCAA
GTTGGACATC
ACCTCCCACA
ACGAGGACTA
CACCATCGTG
GAACAGTACG
AACGCGCCGA
GGGCCGCCAC
TCCACCGGCG
GCATGGACGA
GCTGTACAAG
GCAACAAACT
TCTCTCTGCT
GAAACAAGCC
GGAGATGTCG
AAGAGAATCC
TGGACCGATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGATCT
TATCGATAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAATCATCGT
CCTTTCCTTG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCATCG
ATACCGTCGA
CCAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GGGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATCTGCTTT
TTGCTTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGCATCTAG
AATTAATTCC
GTGTATTCTA
TAGTGTCACC
TAAATCGTAT
GTGTATGATA
CATAAGGTTA
TGTATTAATT
GTAGCCGCGT
TCTAACGACA
ATATGTACAA
GCCTAATTGT
GTAGCATCTG
GCTTACTGAA
GCAGACCCTA
TCATCTCTCT
CGTAAACTGC
CGTCAGAGTC
GGTTTGGTTG
GACGAACCTT
CTGAGTTTCT
GGTAACGCCG
TCCCGCACCC
GGAAATGGTC
AGCGAACCAA
TCAGCAGGGT
CATCGCTAGC
CAGATCCTCT
ACGCCGGACG
CATCGTGGCC
GGCATCACCG
GCGCCACAGG
TGCGGTTGCT
GGCGCCTATA
TCGCCGACAT
CACCGATGGG
GAAGATCGGG
CTCGCCACTT
CGGGCTCATG
AGCGCTTGTT
TCGGCGTGGG
TATGGTGGCA
GGCCCCGTGG
CCGGGGGACT
GTTGGGCGCC
ATCTCCTTGC
ATGCACCATT
CCTTGCGGCG
GCGGTGCTCA
ACGGCCTCAA
CCTACTACTG
GGCTGCTTCC
TAATGCAGGA
GTCGCATAAG
GGAGAGCGTC
GAATGGTGCA
CTCTCAGTAC
AATCTAGCTC
TGATGCCGCA
TAGTTAAGCC
AGCCCCGACA
CCCGCCAACA
CCCGCTGACG
CGCCCTGACG
GGCTTGTCTG
CTCCCGGCAT
CCGCTTACAG
ACAAGCTGTG
ACCGTCTCCG
GGAGCTGCAT
GTGTCAGAGG
TTTTCACCGT
CATCACCGAA
ACGCGCGAGA
CGAAAGGGCC
TCGTGATACG
CCTATTTTTA
TAGGTTAATG
TCATGATAAT
AATGGTTTCT
TAGACGTCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TATTGAAAAA
GGAAGAGTAT
GAGTATTCAA
CATTTCCGTG
TCGCCCTTAT
TCCCTTTTTT
GCGGCATTTT
GCCTTCCTGT
TTTTGCTCAC
CCAGAAACGC
TGGTGAAAGT
AAAAGATGCT
GAAGATCAGT
TGGGTGCACG
AGTGGGTTAC
ATCGAACTGG
ATCTCAACAG
CGGTAAGATC
CTTGAGAGTT
TTCGCCCCGA
AGAACGTTTT
CCAATGATGA
GCACTTTTAA
AGTTCTGCTA
TGTGGCGCGG
TATTATCCCG
TATTGACGCC
GGGCAAGAGC
AACTCGGTCG
CCGCATACAC
TATTCTCAGA
ATGACTTGGT
TGAGTACTCA
CCAGTCACAG
AAAAGCATCT
TACGGATGGC
ATGACAGTAA
GAGAATTATG
CAGTGCTGCC
ATAACCATGA
GTGATAACAC
TGCGGCCAAC
TTACTTCTGA
CAACGATCGG
AGGACCGAAG
GAGCTAACCG
CTTTTTTGCA
CAACATGGGG
GATCATGTAA
CTCGCCTTGA
TCGTTGGGAA
CCGGAGCTGA
ATGAAGCCAT
ACCAAACGAC
GAGCGTGACA
CCACGATGCC
TGTAGCAATG
GCAACAACGT
TGCGCAAACT
ATTAACTGGC
GAACTACTTA
CTCTAGCTTC
CCGGCAACAA
TTAATAGACT
GGATGGAGGC
GGATAAAGTT
GCAGGACCAC
TTCTGCGCTC
GGCCCTTCCG
GCTGGCTGGT
TTATTGCTGA
TAAATCTGGA
GCCGGTGAGC
GTGGGTCTCG
CGGTATCATT
GCAGCACTGG
GGCCAGATGG
TAAGCCCTCC
CGTATCGTAG
TTATCTACAC
GACGGGGAGT
CAGGCAACTA
TGGATGAACG
AAATAGACAG
ATCGCTGAGA
TAGGTGCCTC
ACTGATTAAG
CATTGGTAAC
TGTCAGACCA
AGTTTACTCA
TATATACTTT
AGATTGATTT
AAAACTTCAT
TTTTAATTTA
AAAGGATCTA
GGTGAAGATC
CTTTTTGATA
ATCTCATGAC
CAAAATCCCT
TAACGTGAGT
TTTCGTTCCA
CTGAGCGTCA
GACCCCGTAG
AAAAGATCAA
AGGATCTTCT
TGAGATCCTT
TTTTTCTGCG
CGTAATCTGC
TGCTTGCAAA
CAAAAAAACC
ACCGCTACCA
GCGGTGGTTT
GTTTGCCGGA
TCAAGAGCTA
CCAACTCTTT
TTCCGAAGGT
AACTGGCTTC
AGCAGAGCGC
AGATACCAAA
TACTGTCCTT
CTAGTGTAGC
CGTAGTTAGG
CCACCACTTC
AAGAACTCTG
TAGCACCGCC
TACATACCTC
GCTCTGCTAA
TCCTGTTACC
AGTGGCTGCT
GCCAGTGGCG
ATAAGTCGTG
TCTTACCGGG
TTGGACTCAA
GACGATAGTT
ACCGGATAAG
GCGCAGCGGT
CGGGCTGAAC
GGGGGGTTCG
TGCACACAGC
CCAGCTTGGA
GCGAACGACC
TACACCGAAC
TGAGATACCT
ACAGCGTGAG
CTATGAGAAA
GCGCCACGCT
TCCCGAAGGG
AGAAAGGCGG
ACAGGTATCC
GGTAAGCGGC
AGGGTCGGAA
CAGGAGAGCG
CACGAGGGAG
CTTCCAGGGG
GAAACGCCTG
GTATCTTTAT
AGTCCTGTCG
GGTTTCGCCA
CCTCTGACTT
GAGCGTCGAT
TTTTGTGATG
CTCGTCAGGG
GGGCGGAGCC
TATGGAAAAA
CGCCAGCAAC
GCGGCCTTTT
TACGGTTCCT
GGCCTTTTGC
TGGCCTTTTG
CTCACATGTT
CTTTCCTGCG
TTATCCCCTG
ATTCTGTGGA
TAACCGTATT
ACCGCCTTTG
AGTGAGCTGA
TACCGCTCGC
CGCAGCCGAA
CGACCGAGCG
CAGCGAGTCA
GTGAGCGAGG
AAGCGGAAGA
GCGCCCAATA
CGCAAACCGC
CTCTCCCCGC
GCGTTGGCCG
ATTCATTAAT
GCAGCTGTGG
AATGTGTGTC
AGTTAGGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCAGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
TAGTCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCGGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGCTTTTGC
AAAAAGCTTG
GACACAAGAC
AGGCTTGCGA
GATATGTTTG
AGAATACCAC
TTTATCCCGC
GTCAGGGAGA
GGCAGTGCGT
AAAAAGACGC
GGACTCATGT
GAAATACTGG
TTTTTAGTGC
GCCAGATCTC
TATAATCTCG
CGCAACCTAT
TTTCCCCTCG
AACACTTTTT
AAGCCGTAGA
TAAACAGGCT
GGGACACTTC
ACATGAGCGA
AAAATACATC
GTCACCTGGG
ACATGTTGCA
GATCCATGCA
CGTAAACTCG
CAAGCCGACT
GATGCCTTCT
GAACAATGGA
AAGGCATTAT
TGCCGTAAGC
CGTGGCGGTC
TGTACCGGGT
GCGTTACTGG
CGCGTGAACT
GGGTATTCGT
CATGTCGATA
CCGTTTGTAT
TTCCAGCTAC
GATCACGACA
ACCAGCGCGA
GCTTAAAGTG
CTGAAACGCG
CAGAAGGCGA
TGGCGAAGGC
TTCATCGTTA
TTGATGACCT
GGTGGATACC
GGTGGTACTG
CGGTTGCGAT
TCGTGAAATG
TATCCAAAAG
CGCACTTTGT
CACCATCTTC
GCAAAACCGG
CTGGTCGTCC
GCTGGTTGAT
GACTATGTTG
TTGATATCCC
GCAAGATACC
TGGATTGAAC
AGCCGTGGGA
TATGGGCGTC
GTATTCGTCC
CGCCAATCTC
CGGTCGCTAA
TCTTTTCAAC
GCCTGGCACT
GCCGGGCGTT
GTTCTTTTTA
ACTTCAGGCG
GGTTACAATA
GTTTCCAGTA
AGTATTCTGG
AGGCTGCATC
CATGACACAG
GCAAACCTGA
GCGAAACCCT
GTTCAAACCC
CGCTTTAAAC
ATCCTGAAAC
CTCGACGCTA
GTCCGCCGCT
TTAATCACGG
CGCACAACCG
CCTGTGCAGT
CGGCCCTTGA
TGGTAAAACC
ATCCCTCACT
GGTATCGCAT
GATTAACCGT
CTGATGTGGA
TCTGGCGCGG
CATTGACCCA
CGCGAAATCC
TCGACGTCCA
GGCACGTATT
GTGATGAGCG
ATGCCGAACG
TACCGACGAT
GATTTATACG
ATACGGTGAT
TGGCTACCGT
GGCGGCAACT
GGATTTATGA
GTGGGCCCCG
GATCTTTGTG
AAGGAACCTT
ACTTCTGTGG
TGTGACATAA
TTGGACAAAC
TACCTACAGA
GATTTAAAGC
TCTAAGGTAA
ATATAAAATT
TTTAAGTGTA
TAATGTGTTA
AACTACTGAT
TCTAATTGTT
TGTGTATTTT
AGATTCCAAC
CTATGGAACT
GATGAATGGG
AGCAGTGGTG
GAATGCCTTT
AATGAGGAAA
ACCTGTTTTG
CTCAGAAGAA
ATGCCATCTA
GTGATGATGA
GGCTACTGCT
GACTCTCAAC
ATTCTACTCC
TCCAAAAAAG
AAGAGAAAGG
TAGAAGACCC
CAAGGACTTT
CCTTCAGAAT
TGCTAAGTTT
TTTGAGTCAT
GCTGTGTTTA
GTAATAGAAC
TCTTGCTTGC
TTTGCTATTT
ACACCACAAA
GGAAAAAGCT
GCACTGCTAT
ACAAGAAAAT
TATGGAAAAA
TATTCTGTAA
CCTTTATAAG
TAGGCATAAC
AGTTATAATC
ATAACATACT
GTTTTTTCTT
ACTCCACACA
GGCATAGAGT
GTCTGCTATT
AATAACTATG
CTCAAAAATT
GTGTACCTTT
AGCTTTTTAA
TTTGTAAAGG
GGTTAATAAG
GAATATTTGA
TGTATAGTGC
CTTGACTAGA
GATCATAATC
AGCCATACCA
CATTTGTAGA
GGTTTTACTT
GCTTTAAAAA
ACCTCCCACA
CCTCCCCCTG
AACCTGAAAC
ATAAAATGAA
TGCAATTGTT
GTTGTTAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGGAT
CAACTGGATA
ACTCAAGCTA
ACCAAAATCA
TCCCAAACTT
CCCACCCCAT
ACCCTATTAC
CACTGCCAAT
TACCTAGTGG
TTTCATTTAC
TCTAAACCTG
TGATTCCTCT
GAATTATTTT
CATTTTAAAG
AAATTGTATT
TGTTAAATAT
GTACTACAAA
CTTAGTAGTT
GGAAGGGCTA
ATTCACTCCC
AAAGAAGACA
AGATATCCTT
GATCTGTGGA
TCTACCACAC
ACAAGGCTAC
TTCCCTGATT
AGCAGAACTA
CACACCAGGG
CCAGGGGTCA
GATATCCACT
GACCTTTGGA
TGGTGCTACA
AGCTAGTACC
AGTTGAGCCA
GATAAGGTAG
AAGAGGCCAA
TAAAGGAGAG
AACACCAGCT
TGTTACACCC
TGTGAGCCTG
CATGGGATGG
ATGACCCGGA
GAGAGAAGTG
TTAGAGTGGA
GGTTTGACAG
CCGCCTAGCA
TTTCATCACG
TGGCCCGAGA
GCTGCATCCG
GAGTACTTCA
AGAACTGCTG
ATATCGAGCT
TGCTACAAGG
GACTTTCCGC
TGGGGACTTT
CCAGGGAGGC
GTGGCCTGGG
CGGGACTGGG
GAGTGGCGAG
CCCTCAGATC
CTGCATATAA
GCAGCTGCTT
TTTGCCTGTA
CTGGGTCTCT
CTGGTTAGAC
CAGATCTGAG
CCTGGGAGCT
CTCTGGCTAA
CTAGGGAACC
CACTGCTTAA
GCCTCAATAA
AGCTTGCCTT
GAGTGCTTCA
AGTAGTGTGT
GCCCGTCTGT
TGTGTGACTC
TGGTAACTAG
AGATCCCTCA
GACCCTTTTA
GTCAGTGTGG
AAAATCTCTA
GCAGTGGCGC
CCGAACAGGG
ACTTGAAAGC
GAAAGGGAAA
CCAGAGGAGC
TCTCTCGACG
CAGGACTCGG
CTTGCTGAAG
CGCGCACGGC
AAGAGGCGAG
GGGCGGCGAC
TGGTGAGTAC
GCCAAAAATT
TTGACTAGCG
GAGGCTAGAA
GGAGAGAGAT
GGGTGCGAGA
GCGTCAGTAT
TAAGCGGGGG
AGAATTAGAT
CGCGATGGGA
AAAAATTCGG
TTAAGGCCAG
GGGGAAAGAA
AAAATATAAA
TTAAAACATA
TAGTATGGGC
AAGCAGGGAG
CTAGAACGAT
TCGCAGTTAA
TCCTGGCCTG
TTAGAAACAT
CAGAAGGCTG
TAGACAAATA
CTGGGACAGC
TACAACCATC
CCTTCAGACA
GGATCAGAAG
AACTTAGATC
ATTATATAAT
ACAGTAGCAA
CCCTCTATTG
TGTGCATCAA
AGGATAGAGA
TAAAAGACAC
CAAGGAAGCT
TTAGACAAGA
TAGAGGAAGA
GCAAAACAAA
AGTAAGACCA
CCGCACAGCA
AGCGGCCGGT
GATCTTCAGA
CCTGGACGAT
ATATATGAGG
GACAATTGGA
GAAGTGAATT
ATATAAATAT
AAAGTAGTAA
AAATTGAACC
ATTAGGAGTA
GCACCCACCA
AGGCAAAGAG
AAGAGTGGTG
CAGAGAGAAA
AAAGAGCAGT
GGGAATAGGA
GCTTTGTTCC
TTGGGTTCTT
GGGAGCAGCA
GGAAGCACTA
TGGGCGCAGC
GTCAATGACG
CTGACGGTAC
AGGCCAGACA
ATTATTGTCT
GGTATAGTGC
AGCAGCAGAA
CAATTTGCTG
AGGGCTATTG
AGGCGCAACA
GCATCTGTTG
CAACTCACAG
TCTGGGGCAT
CAAGCAGCTC
CAGGCAAGAA
TCCTGGCTGT
GGAAAGATAC
CTAAAGGATC
AACAGCTCCT
GGGGATTTGG
GGTTGCTCTG
GAAAACTCAT
TTGCACCACT
GCTGTGCCTT
GGAATGCTAG
TTGGAGTAAT
AAATCTCTGG
AACAGATTTG
GAATCACACG
ACCTGGATGG
AGTGGGACAG
AGAAATTAAC
AATTACACAA
GCTTAATACA
CTCCTTAATT
GAAGAATCGC
AAAACCAGCA
AGAAAAGAAT
GAACAAGAAT
TATTGGAATT
AGATAAATGG
GCAAGTTTGT
GGAATTGGTT
TAACATAACA
AATTGGCTGT
GGTATATAAA
ATTATTCATA
ATGATAGTAG
GAGGCTTGGT
AGGTTTAAGA
ATAGTTTTTG
CTGTACTTTC
TATAGTGAAT
AGAGTTAGGC
AGGGATATTC
ACCATTATCG
TTTCAGACCC
ACCTCCCAAC
CCCGAGGGGA
CCCGACAGGC
CCGAAGGAAT
AGAAGAAGAA
GGTGGAGAGA
GAGACAGAGA
CAGATCCATT
CGATTAGTGA
ACGGATCTCG
ACGGTCG