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pCMV-MORC3(human)-Neo

产品货号:P65140

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCMV-MORC3(human)-Neo
别称: NM_015358.3;NXP2; ZCW5; ZCWCC3
基因长: 2817bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记: Neo/G418
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体: pCDNA3.1(+)
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GCACTCTCAG

TACAATCTGC

TCTGATGCCG

CATAGTTAAG

CCAGTATCTG

CTCCCTGCTT

61

GTGTGTTGGA

GGTCGCTGAG

TAGTGCGCGA

GCAAAATTTA

AGCTACAACA

AGGCAAGGCT

121

TGACCGACAA

TTGCATGAAG

AATCTGCTTA

GGGTTAGGCG

TTTTGCGCTG

CTTCGCGATG

181

TACGGGCCAG

ATATACGCGT

TGACATTGAT

TATTGACTAG

TTATTAATAG

TAATCAATTA

241

CGGGGTCATT

AGTTCATAGC

CCATATATGG

AGTTCCGCGT

TACATAACTT

ACGGTAAATG

301

GCCCGCCTGG

CTGACCGCCC

AACGACCCCC

GCCCATTGAC

GTCAATAATG

ACGTATGTTC

361

CCATAGTAAC

GCCAATAGGG

ACTTTCCATT

GACGTCAATG

GGTGGAGTAT

TTACGGTAAA

421

CTGCCCACTT

GGCAGTACAT

CAAGTGTATC

ATATGCCAAG

TACGCCCCCT

ATTGACGTCA

481

ATGACGGTAA

ATGGCCCGCC

TGGCATTATG

CCCAGTACAT

GACCTTATGG

GACTTTCCTA

541

CTTGGCAGTA

CATCTACGTA

TTAGTCATCG

CTATTACCAT

GGTGATGCGG

TTTTGGCAGT

601

ACATCAATGG

GCGTGGATAG

CGGTTTGACT

CACGGGGATT

TCCAAGTCTC

CACCCCATTG

661

ACGTCAATGG

GAGTTTGTTT

TGGCACCAAA

ATCAACGGGA

CTTTCCAAAA

TGTCGTAACA

721

ACTCCGCCCC

ATTGACGCAA

ATGGGCGGTA

GGCGTGTACG

GTGGGAGGTC

TATATAAGCA

781

GAGCTCTCTG

GCTAACTAGA

GAACCCACTG

CTTACTGGCT

TATCGAAATT

AATACGACTC

841

ACTATAGGGA

GACCCAAGCT

GGCTAGCCGC

CACCATGGCG

GCGCAGCCAC

CCCGCGGGAT

901

ACGCCTCAGC

GCGCTTTGCC

CGAAGTTTTT

ACATACAAAT

TCTACTAGTC

ACACCTGGCC

961

ATTCAGTGCA

GTTGCTGAAT

TAATAGATAA

TGCTTATGAT

CCTGATGTGA

ACGCTAAACA

1021

AATATGGATT

GACAAAACAG

TGATAAATGA

CCATATATGC

TTGACATTCA

CCGACAATGG

1081

GAATGGTATG

ACTTCTGATA

AATTACATAA

AATGCTAAGC

TTTGGCTTCA

GTGACAAAGT

1141

CACCATGAAT

GGTCATGTCC

CAGTTGGATT

ATATGGGAAT

GGCTTCAAGT

CGGGTTCTAT

1201

GCGTCTGGGT

AAAGACGCAA

TCGTTTTTAC

CAAAAATGGA

GAAAGCATGA

GCGTGGGCCT

1261

TTTGTCTCAG

ACCTACTTGG

AAGTCATAAA

AGCGGAGCAT

GTTGTTGTTC

CAATAGTGGC

1321

ATTCAACAAG

CACCGACAGA

TGATTAATTT

AGCAGAATCA

AAAGCCAGCC

TTGCTGCAAT

1381

TCTGGAACAT

TCTCTGTTTT

CCACGGAACA

GAAGTTACTG

GCAGAACTTG

ATGCTATTAT

1441

AGGCAAGAAG

GGGACGAGGA

TCATCATTTG

GAATCTTAGA

AGCTACAAAA

ATGCAACAGA

1501

GTTCGATTTT

GAAAAGGATA

AATATGATAT

CAGAATTCCC

GAGGATTTAG

ATGAGATAAC

1561

AGGGAAGAAG

GGGTACAAGA

AGCAGGAAAG

GATGGACCAG

ATTGCCCCTG

AGAGTGACTA

1621

TTCCCTGAGG

GCTTATTGCA

GTATATTATA

TCTAAAGCCA

AGAATGCAGA

TCATCCTACG

1681

TGGACAGAAA

GTGAAGACAC

AGCTGGTTTC

GAAGAGTCTT

GCCTACATCG

AACGTGATGT

1741

TTATCGACCA

AAATTTTTAT

CTAAAACAGT

GAGAATTACC

TTTGGATTCA

ACTGCAGAAA

1801

TAAAGATCAT

TATGGGATAA

TGATGTATCA

CAGAAATAGA

CTCATCAAAG

CTTATGAAAA

1861

AGTTGGATGT

CAGTTAAGGG

CAAACAACAT

GGGTGTTGGA

GTGGTTGGAA

TTATAGAGTG

1921

TAATTTCCTT

AAGCCAACTC

ATAATAAACA

AGATTTCGAC

TATACTAATG

AGTACAGACT

1981

TACAATAACA

GCACTAGGAG

AAAAGCTGAA

TGATTACTGG

AATGAAATGA

AAGTGAAGAA

2041

AAATACAGAA

TATCCTCTAA

ATTTGCCAGT

TGAAGATATA

CAGAAGCGTC

CTGATCAGAC

2101

ATGGGTTCAG

TGTGATGCCT

GTCTAAAGTG

GCGGAAATTA

CCTGATGGGA

TGGATCAACT

2161

TCCTGAAAAA

TGGTATTGCT

CCAATAACCC

TGACCCACAG

TTCAGAAATT

GTGAGGTTCC

2221

AGAAGAACCT

GAAGATGAGG

ATTTGGTACA

TCCCACTTAT

GAAAAAACCT

ACAAAAAGAC

2281

CAACAAGGAA

AAATTCAGGA

TCAGACAACC

GGAAATGATC

CCTCGGATTA

ATGCTGAACT

2341

GTTGTTTCGG

CCAACTGCTC

TTTCAACTCC

AAGCTTTTCT

TCTCCTAAGG

AAAGTGTTCC

2401

AAGAAGACAT

CTTTCAGAAG

GAACAAATTC

TTATGCGACA

AGACTTCTAA

ATAATCATCA

2461

AGTTCCACCT

CAGTCTGAAC

CTGAGAGCAA

CAGCTTGAAA

CGGAGACTTT

CTACTCGTTC

2521

CTCAATTTTG

AATGCAAAGA

ATCGGAGATT

GAGTAGTCAG

TTTGAAAATT

CAGTTTATAA

2581

AGGTGATGAT

GATGATGAAG

ATGTCATCAT

CTTAGAAGAA

AACAGTACCC

CCAAACCTGC

2641

AGTAGATCAT

GATATTGACA

TGAAATCAGA

ACAGAGTCAC

GTTGAGCAAG

GTGGTGTTCA

2701

GGTTGAGTTT

GTGGGTGACA

GTGAACCTTG

TGGCCAGACT

GGTTCAACAA

GCACCTCATC

2761

ATCCCGATGC

GACCAGGGAA

ATACTGCAGC

TACCCAGACT

GAAGTACCAA

GTTTAGTTGT

2821

TAAAAAAGAA

GAAACTGTTG

AAGACGAGAT

AGACGTAAGA

AATGATGCAG

TGATTCTGCC

2881

CTCCTGTGTA

GAAGCTGAAG

CAAAGATACA

TGAAACCCAG

GAAACCACCG

ATAAATCTGC

2941

AGATGATGCA

GGCTGCCAAT

TACAAGAACT

GAGAAACCAG

CTACTCCTTG

TCACTGAGGA

3001

AAAAGAGAAT

TATAAAAGAC

AGTGTCATAT

GTTTACTGAT

CAAATCAAAG

TGTTACAACA

3061

GAGGATACTA

GAAATGAATG

ACAAGTATGT

TAAGAAAGAA

ACTTGCCATC

AGTCCACTGA

3121

AACCGATGCT

GTATTTTTAC

TTGAAAGTAT

TAATGGCAAA

TCTGAAAGTC

CAGACCATAT

3181

GGTATCTCAG

TATCAGCAAG

CTTTGGAAGA

AATAGAAAGG

CTGAAAAAAC

AATGTAGTGC

3241

TTTGCAACAT

GTAAAGGCTG

AATGCAGCCA

GTGTTCCAAT

AATGAGAGTA

AAAGTGAAAT

3301

GGATGAGATG

GCTGTGCAGC

TTGACGATGT

GTTTAGACAA

CTGGACAAAT

GCAGTATTGA

3361

GAGGGACCAG

TATAAAAGTG

AGGTTGAATT

GCTGGAAATG

GAAAAGTCAC

AAATCCGTTC

3421

ACAGTGTGAA

GAACTCAAAA

CTGAAGTAGA

ACAGTTAAAA

TCTACAAATC

AACAGACGGC

3481

AACAGATGTT

TCAACATCAA

GTAACATTGA

GGAGTCTGTA

AATCATATGG

ATGGAGAAAG

3541

CCTCAAACTC

CGATCTCTTC

GAGTTAACGT

AGGACAACTG

CTGGCTATGA

TTGTGCCTGA

3601

TCTTGATCTT

CAGCAAGTGA

ATTACGATGT

TGATGTAGTT

GATGAGATTT

TAGGACAAGT

3661

TGTTGAACAA

ATGAGTGAAA

TCAGTAGTAC

TTAAGCGGCC

GCTCGAGTCT

AGAGGGCCCG

3721

TTTAAACCCG

CTGATCAGCC

TCGACTGTGC

CTTCTAGTTG

CCAGCCATCT

GTTGTTTGCC

3781

CCTCCCCCGT

GCCTTCCTTG

ACCCTGGAAG

GTGCCACTCC

CACTGTCCTT

TCCTAATAAA

3841

ATGAGGAAAT

TGCATCGCAT

TGTCTGAGTA

GGTGTCATTC

TATTCTGGGG

GGTGGGGTGG

3901

GGCAGGACAG

CAAGGGGGAG

GATTGGGAAG

ACAATAGCAG

GCATGCTGGG

GATGCGGTGG

3961

GCTCTATGGC

TTCTGAGGCG

GAAAGAACCA

GCTGGGGCTC

TAGGGGGTAT

CCCCACGCGC

4021

CCTGTAGCGG

CGCATTAAGC

GCGGCGGGTG

TGGTGGTTAC

GCGCAGCGTG

ACCGCTACAC

4081

TTGCCAGCGC

CCTAGCGCCC

GCTCCTTTCG

CTTTCTTCCC

TTCCTTTCTC

GCCACGTTCG

4141

CCGGCTTTCC

CCGTCAAGCT

CTAAATCGGG

GGCTCCCTTT

AGGGTTCCGA

TTTAGTGCTT

4201

TACGGCACCT

CGACCCCAAA

AAACTTGATT

AGGGTGATGG

TTCACGTAGT

GGGCCATCGC

4261

CCTGATAGAC

GGTTTTTCGC

CCTTTGACGT

TGGAGTCCAC

GTTCTTTAAT

AGTGGACTCT

4321

TGTTCCAAAC

TGGAACAACA

CTCAACCCTA

TCTCGGTCTA

TTCTTTTGAT

TTATAAGGGA

4381

TTTTGCCGAT

TTCGGCCTAT

TGGTTAAAAA

ATGAGCTGAT

TTAACAAAAA

TTTAACGCGA

4441

ATTAATTCTG

TGGAATGTGT

GTCAGTTAGG

GTGTGGAAAG

TCCCCAGGCT

CCCCAGCAGG

4501

CAGAAGTATG

CAAAGCATGC

ATCTCAATTA

GTCAGCAACC

AGGTGTGGAA

AGTCCCCAGG

4561

CTCCCCAGCA

GGCAGAAGTA

TGCAAAGCAT

GCATCTCAAT

TAGTCAGCAA

CCATAGTCCC

4621

GCCCCTAACT

CCGCCCATCC

CGCCCCTAAC

TCCGCCCAGT

TCCGCCCATT

CTCCGCCCCA

4681

TGGCTGACTA

ATTTTTTTTA

TTTATGCAGA

GGCCGAGGCC

GCCTCTGCCT

CTGAGCTATT

4741

CCAGAAGTAG

TGAGGAGGCT

TTTTTGGAGG

CCTAGGCTTT

TGCAAAAAGC

TCCCGGGAGC

4801

TTGTATATCC

ATTTTCGGAT

CTGATCAAGA

GACAGGATGA

GGATCGTTTC

GCATGATTGA

4861

ACAAGATGGA

TTGCACGCAG

GTTCTCCGGC

CGCTTGGGTG

GAGAGGCTAT

TCGGCTATGA

4921

CTGGGCACAA

CAGACAATCG

GCTGCTCTGA

TGCCGCCGTG

TTCCGGCTGT

CAGCGCAGGG

4981

GCGCCCGGTT

CTTTTTGTCA

AGACCGACCT

GTCCGGTGCC

CTGAATGAAC

TGCAGGACGA

5041

GGCAGCGCGG

CTATCGTGGC

TGGCCACGAC

GGGCGTTCCT

TGCGCAGCTG

TGCTCGACGT

5101

TGTCACTGAA

GCGGGAAGGG

ACTGGCTGCT

ATTGGGCGAA

GTGCCGGGGC

AGGATCTCCT

5161

GTCATCTCAC

CTTGCTCCTG

CCGAGAAAGT

ATCCATCATG

GCTGATGCAA

TGCGGCGGCT

5221

GCATACGCTT

GATCCGGCTA

CCTGCCCATT

CGACCACCAA

GCGAAACATC

GCATCGAGCG

5281

AGCACGTACT

CGGATGGAAG

CCGGTCTTGT

CGATCAGGAT

GATCTGGACG

AAGAGCATCA

5341

GGGGCTCGCG

CCAGCCGAAC

TGTTCGCCAG

GCTCAAGGCG

CGCATGCCCG

ACGGCGAGGA

5401

TCTCGTCGTG

ACCCATGGCG

ATGCCTGCTT

GCCGAATATC

ATGGTGGAAA

ATGGCCGCTT

5461

TTCTGGATTC

ATCGACTGTG

GCCGGCTGGG

TGTGGCGGAC

CGCTATCAGG

ACATAGCGTT

5521

GGCTACCCGT

GATATTGCTG

AAGAGCTTGG

CGGCGAATGG

GCTGACCGCT

TCCTCGTGCT

5581

TTACGGTATC

GCCGCTCCCG

ATTCGCAGCG

CATCGCCTTC

TATCGCCTTC

TTGACGAGTT

5641

CTTCTGAGCG

GGACTCTGGG

GTTCGAAATG

ACCGACCAAG

CGACGCCCAA

CCTGCCATCA

5701

CGAGATTTCG

ATTCCACCGC

CGCCTTCTAT

GAAAGGTTGG

GCTTCGGAAT

CGTTTTCCGG

5761

GACGCCGGCT

GGATGATCCT

CCAGCGCGGG

GATCTCATGC

TGGAGTTCTT

CGCCCACCCC

5821

AACTTGTTTA

TTGCAGCTTA

TAATGGTTAC

AAATAAAGCA

ATAGCATCAC

AAATTTCACA

5881

AATAAAGCAT

TTTTTTCACT

GCATTCTAGT

TGTGGTTTGT

CCAAACTCAT

CAATGTATCT

5941

TATCATGTCT

GTATACCGTC

GACCTCTAGC

TAGAGCTTGG

CGTAATCATG

GTCATAGCTG

6001

TTTCCTGTGT

GAAATTGTTA

TCCGCTCACA

ATTCCACACA

ACATACGAGC

CGGAAGCATA

6061

AAGTGTAAAG

CCTGGGGTGC

CTAATGAGTG

AGCTAACTCA

CATTAATTGC

GTTGCGCTCA

6121

CTGCCCGCTT

TCCAGTCGGG

AAACCTGTCG

TGCCAGCTGC

ATTAATGAAT

CGGCCAACGC

6181

GCGGGGAGAG

GCGGTTTGCG

TATTGGGCGC

TCTTCCGCTT

CCTCGCTCAC

TGACTCGCTG

6241

CGCTCGGTCG

TTCGGCTGCG

GCGAGCGGTA

TCAGCTCACT

CAAAGGCGGT

AATACGGTTA

6301

TCCACAGAAT

CAGGGGATAA

CGCAGGAAAG

AACATGTGAG

CAAAAGGCCA

GCAAAAGGCC

6361

AGGAACCGTA

AAAAGGCCGC

GTTGCTGGCG

TTTTTCCATA

GGCTCCGCCC

CCCTGACGAG

6421

CATCACAAAA

ATCGACGCTC

AAGTCAGAGG

TGGCGAAACC

CGACAGGACT

ATAAAGATAC

6481

CAGGCGTTTC

CCCCTGGAAG

CTCCCTCGTG

CGCTCTCCTG

TTCCGACCCT

GCCGCTTACC

6541

GGATACCTGT

CCGCCTTTCT

CCCTTCGGGA

AGCGTGGCGC

TTTCTCATAG

CTCACGCTGT

6601

AGGTATCTCA

GTTCGGTGTA

GGTCGTTCGC

TCCAAGCTGG

GCTGTGTGCA

CGAACCCCCC

6661

GTTCAGCCCG

ACCGCTGCGC

CTTATCCGGT

AACTATCGTC

TTGAGTCCAA

CCCGGTAAGA

6721

CACGACTTAT

CGCCACTGGC

AGCAGCCACT

GGTAACAGGA

TTAGCAGAGC

GAGGTATGTA

6781

GGCGGTGCTA

CAGAGTTCTT

GAAGTGGTGG

CCTAACTACG

GCTACACTAG

AAGAACAGTA

6841

TTTGGTATCT

GCGCTCTGCT

GAAGCCAGTT

ACCTTCGGAA

AAAGAGTTGG

TAGCTCTTGA

6901

TCCGGCAAAC

AAACCACCGC

TGGTAGCGGT

TTTTTTGTTT

GCAAGCAGCA

GATTACGCGC

6961

AGAAAAAAAG

GATCTCAAGA

AGATCCTTTG

ATCTTTTCTA

CGGGGTCTGA

CGCTCAGTGG

7021

AACGAAAACT

CACGTTAAGG

GATTTTGGTC

ATGAGATTAT

CAAAAAGGAT

CTTCACCTAG

7081

ATCCTTTTAA

ATTAAAAATG

AAGTTTTAAA

TCAATCTAAA

GTATATATGA

GTAAACTTGG

7141

TCTGACAGTT

ACCAATGCTT

AATCAGTGAG

GCACCTATCT

CAGCGATCTG

TCTATTTCGT

7201

TCATCCATAG

TTGCCTGACT

CCCCGTCGTG

TAGATAACTA

CGATACGGGA

GGGCTTACCA

7261

TCTGGCCCCA

GTGCTGCAAT

GATACCGCGA

GACCCACGCT

CACCGGCTCC

AGATTTATCA

7321

GCAATAAACC

AGCCAGCCGG

AAGGGCCGAG

CGCAGAAGTG

GTCCTGCAAC

TTTATCCGCC

7381

TCCATCCAGT

CTATTAATTG

TTGCCGGGAA

GCTAGAGTAA

GTAGTTCGCC

AGTTAATAGT

7441

TTGCGCAACG

TTGTTGCCAT

TGCTACAGGC

ATCGTGGTGT

CACGCTCGTC

GTTTGGTATG

7501

GCTTCATTCA

GCTCCGGTTC

CCAACGATCA

AGGCGAGTTA

CATGATCCCC

CATGTTGTGC

7561

AAAAAAGCGG

TTAGCTCCTT

CGGTCCTCCG

ATCGTTGTCA

GAAGTAAGTT

GGCCGCAGTG

7621

TTATCACTCA

TGGTTATGGC

AGCACTGCAT

AATTCTCTTA

CTGTCATGCC

ATCCGTAAGA

7681

TGCTTTTCTG

TGACTGGTGA

GTACTCAACC

AAGTCATTCT

GAGAATAGTG

TATGCGGCGA

7741

CCGAGTTGCT

CTTGCCCGGC

GTCAATACGG

GATAATACCG

CGCCACATAG

CAGAACTTTA

7801

AAAGTGCTCA

TCATTGGAAA

ACGTTCTTCG

GGGCGAAAAC

TCTCAAGGAT

CTTACCGCTG

7861

TTGAGATCCA

GTTCGATGTA

ACCCACTCGT

GCACCCAACT

GATCTTCAGC

ATCTTTTACT

7921

TTCACCAGCG

TTTCTGGGTG

AGCAAAAACA

GGAAGGCAAA

ATGCCGCAAA

AAAGGGAATA

7981

AGGGCGACAC

GGAAATGTTG

AATACTCATA

CTCTTCCTTT

TTCAATATTA

TTGAAGCATT

8041

TATCAGGGTT

ATTGTCTCAT

GAGCGGATAC

ATATTTGAAT

GTATTTAGAA

AAATAAACAA

8101

ATAGGGGTTC

CGCGCACATT

TCCCCGAAAA

GTGCCACCTG

ACGTCGACGG

ATCGGGAGAT

8161

CTCCCGATCC

CCTATGGT

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