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ARR3tk-MCS-SV40-mCherry

产品货号:P65081

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: ARR3tk-MCS-SV40-mCherry
别称: -
基因长:
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 转录因子报告
片段类型: Element
片段物种: 空载体
原核抗性: Amp
筛选标记:
荧光标记: 红色
启动子:
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体: ARR3tk-eGFP/SV40-mCherry
正向引物:
反向引物: WPRE-R
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CATGTTCTTT

CCTGCGTTAT

CCCCTGATTC

TGTGGATAAC

CGTATTACCG

CCTTTGAGTG

61

AGCTGATACC

GCTCGCCGCA

GCCGAACGAC

CGAGCGCAGC

GAGTCAGTGA

GCGAGGAAGC

121

GGAAGAGCGC

CCAATACGCA

AACCGCCTCT

CCCCGCGCGT

TGGCCGATTC

ATTAATGCAG

181

CTGGCACGAC

AGGTTTCCCG

ACTGGAAAGC

GGGCAGTGAG

CGCAACGCAA

TTAATGTGAG

241

TTAGCTCACT

CATTAGGCAC

CCCAGGCTTT

ACACTTTATG

CTTCCGGCTC

GTATGTTGTG

301

TGGAATTGTG

AGCGGATAAC

AATTTCACAC

AGGAAACAGC

TATGACCATG

ATTACGCCAA

361

GCGCGCAATT

AACCCTCACT

AAAGGGAACA

AAAGCTGGAG

CTGCAAGCTT

AATGTAGTCT

421

TATGCAATAC

TCTTGTAGTC

TTGCAACATG

GTAACGATGA

GTTAGCAACA

TGCCTTACAA

481

GGAGAGAAAA

AGCACCGTGC

ATGCCGATTG

GTGGAAGTAA

GGTGGTACGA

TCGTGCCTTA

541

TTAGGAAGGC

AACAGACGGG

TCTGACATGG

ATTGGACGAA

CCACTGAATT

GCCGCATTGC

601

AGAGATATTG

TATTTAAGTG

CCTAGCTCGA

TACATAAACG

GGTCTCTCTG

GTTAGACCAG

661

ATCTGAGCCT

GGGAGCTCTC

TGGCTAACTA

GGGAACCCAC

TGCTTAAGCC

TCAATAAAGC

721

TTGCCTTGAG

TGCTTCAAGT

AGTGTGTGCC

CGTCTGTTGT

GTGACTCTGG

TAACTAGAGA

781

TCCCTCAGAC

CCTTTTAGTC

AGTGTGGAAA

ATCTCTAGCA

GTGGCGCCCG

AACAGGGACT

841

TGAAAGCGAA

AGGGAAACCA

GAGGAGCTCT

CTCGACGCAG

GACTCGGCTT

GCTGAAGCGC

901

GCACGGCAAG

AGGCGAGGGG

CGGCGACTGG

TGAGTACGCC

AAAAATTTTG

ACTAGCGGAG

961

GCTAGAAGGA

GAGAGATGGG

TGCGAGAGCG

TCAGTATTAA

GCGGGGGAGA

ATTAGATCGC

1021

GATGGGAAAA

AATTCGGTTA

AGGCCAGGGG

GAAAGAAAAA

ATATAAATTA

AAACATATAG

1081

TATGGGCAAG

CAGGGAGCTA

GAACGATTCG

CAGTTAATCC

TGGCCTGTTA

GAAACATCAG

1141

AAGGCTGTAG

ACAAATACTG

GGACAGCTAC

AACCATCCCT

TCAGACAGGA

TCAGAAGAAC

1201

TTAGATCATT

ATATAATACA

GTAGCAACCC

TCTATTGTGT

GCATCAAAGG

ATAGAGATAA

1261

AAGACACCAA

GGAAGCTTTA

GACAAGATAG

AGGAAGAGCA

AAACAAAAGT

AAGACCACCG

1321

CACAGCAAGC

GGCCGCTGAT

CTTCAGACCT

GGAGGAGGAG

ATATGAGGGA

CAATTGGAGA

1381

AGTGAATTAT

ATAAATATAA

AGTAGTAAAA

ATTGAACCAT

TAGGAGTAGC

ACCCACCAAG

1441

GCAAAGAGAA

GAGTGGTGCA

GAGAGAAAAA

AGAGCAGTGG

GAATAGGAGC

TTTGTTCCTT

1501

GGGTTCTTGG

GAGCAGCAGG

AAGCACTATG

GGCGCAGCGT

CAATGACGCT

GACGGTACAG

1561

GCCAGACAAT

TATTGTCTGG

TATAGTGCAG

CAGCAGAACA

ATTTGCTGAG

GGCTATTGAG

1621

GCGCAACAGC

ATCTGTTGCA

ACTCACAGTC

TGGGGCATCA

AGCAGCTCCA

GGCAAGAATC

1681

CTGGCTGTGG

AAAGATACCT

AAAGGATCAA

CAGCTCCTGG

GGATTTGGGG

TTGCTCTGGA

1741

AAACTCATTT

GCACCACTGC

TGTGCCTTGG

AATGCTAGTT

GGAGTAATAA

ATCTCTGGAA

1801

CAGATTTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

TTACACAAGC

1861

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

ACAAGAATTA

1921

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

TTGGCTGTGG

1981

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

AGTTTTTGCT

2041

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

TCAGACCCAC

2101

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

TGGAGAGAGA

2161

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGGTT

AACTTTTAAA

2221

AGAAAAGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

AATAGCAACA

2281

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAA

TTCAAAATTT

TATGGATCCA

2341

AGCTCAGATC

CAAGCTTGGA

GCTTATGATA

GCATCTTGTT

CTTAGTCTTT

TTCTTAATAG

2401

GGACATAAAG

CCCACAAATA

AAAATATGCC

TGAAGAATGG

GACAGGCATT

GGGCATTGTC

2461

CATGCCTAGT

AAAGTACTCC

AAGAACCTAT

TTGTATACTA

GATGACACAA

TGTTCTAGCC

2521

AAGCTTGGAG

CTTATGATAG

CATCTTGTTC

TTAGTCTTTT

TCTTAATAGG

GACATAAAGC

2581

CCACAAATAA

AAATATGCCT

GAAGAATGGG

ACAGGCATTG

GGCATTGTCC

ATGCCTAGTA

2641

AAGTACTCCA

AGAACCTATT

TGTATACTAG

ATGACACAAT

GTTCTAGCCA

AGCTTATGAT

2701

AGCATCTTGT

TCTTAGTCTT

TTTCTTAATA

GGGACATAAA

GCCCACAAAT

AAAAATATGC

2761

CTGAAGAATG

GGACAGGCAT

TGGGCATTGT

CCATGCCTAG

TAAAGTACTC

CAAGAACCTA

2821

TTTGTATACT

AGATGACACA

ATGTTCTAGG

GGTACCGAGC

TCGAGCCCGG

GCTCGAGATC

2881

TAGACTCTAG

AGGGTATATA

ATGGAAGCTC

GAATTCCAGC

TTGGCATTCC

GGTACTGTTG

2941

GTAAAAAGCT

TGGCATTCCG

GTACTGTTGG

TAAAGCTAGC

GTATCCACTA

GTCAGATATC

3001

AGCGGCCGCG

ACTCTAGAGT

CGAGAAGCTT

GATGATCTGC

GCAGCACCAT

GGCCTGAAAT

3061

AACCTCTGAA

AGAGGAACTT

GGTTAGGTAC

CTTCTGAGGC

GGAAAGAACC

AGCTGTGGAA

3121

TGTGTGTCAG

TTAGGGTGTG

GAAAGTCCCC

AGGCTCCCCA

GCAGGCAGAA

GTATGCAAAG

3181

CATGCATCTC

AATTAGTCAG

CAACCAGGTG

TGGAAAGTCC

CCAGGCTCCC

CAGCAGGCAG

3241

AAGTATGCAA

AGCATGCATC

TCAATTAGTC

AGCAACCATA

GTCCCGCCCC

TAACTCCGCC

3301

CATCCCGCCC

CTAACTCCGC

CCAGTTCCGC

CCATTCTCCG

CCCCATGGCT

GACTAATTTT

3361

TTTTATTTAT

GCAGAGGCCG

AGGCCGCCTC

GGCCTCTGAG

CTATTCCAGA

AGTAGTGAGG

3421

AGGCTTTTTT

GGAGGCCTAG

GCTTTTGCAA

AAAGCTTACC

ATGGTGAGCA

AGGGCGAGGA

3481

GGATAACATG

GCCATCATCA

AGGAGTTCAT

GCGCTTCAAG

GTGCACATGG

AGGGCTCCGT

3541

GAACGGCCAC

GAGTTCGAGA

TCGAGGGCGA

GGGCGAGGGC

CGCCCCTACG

AGGGCACCCA

3601

GACCGCCAAG

CTGAAGGTGA

CCAAGGGTGG

CCCCCTGCCC

TTCGCCTGGG

ACATCCTGTC

3661

CCCTCAGTTC

ATGTACGGCT

CCAAGGCCTA

CGTGAAGCAC

CCCGCCGACA

TCCCCGACTA

3721

CTTGAAGCTG

TCCTTCCCCG

AGGGCTTCAA

GTGGGAGCGC

GTGATGAACT

TCGAGGACGG

3781

CGGCGTGGTG

ACCGTGACCC

AGGACTCCTC

CCTGCAGGAC

GGCGAGTTCA

TCTACAAGGT

3841

GAAGCTGCGC

GGCACCAACT

TCCCCTCCGA

CGGCCCCGTA

ATGCAGAAGA

AGACCATGGG

3901

CTGGGAGGCC

TCCTCCGAGC

GGATGTACCC

CGAGGACGGC

GCCCTGAAGG

GCGAGATCAA

3961

GCAGAGGCTG

AAGCTGAAGG

ACGGCGGCCA

CTACGACGCT

GAGGTCAAGA

CCACCTACAA

4021

GGCCAAGAAG

CCCGTGCAGC

TGCCCGGCGC

CTACAACGTC

AACATCAAGT

TGGACATCAC

4081

CTCCCACAAC

GAGGACTACA

CCATCGTGGA

ACAGTACGAA

CGCGCCGAGG

GCCGCCACTC

4141

CACCGGCGGC

ATGGACGAGC

TGTACAAGTA

AGTCGACAAT

CAACCTCTGG

ATTACAAAAT

4201

TTGTGAAAGA

TTGACTGGTA

TTCTTAACTA

TGTTGCTCCT

TTTACGCTAT

GTGGATACGC

4261

TGCTTTAATG

CCTTTGTATC

ATGCTATTGC

TTCCCGTATG

GCTTTCATTT

TCTCCTCCTT

4321

GTATAAATCC

TGGTTGCTGT

CTCTTTATGA

GGAGTTGTGG

CCCGTTGTCA

GGCAACGTGG

4381

CGTGGTGTGC

ACTGTGTTTG

CTGACGCAAC

CCCCACTGGT

TGGGGCATTG

CCACCACCTG

4441

TCAGCTCCTT

TCCGGGACTT

TCGCTTTCCC

CCTCCCTATT

GCCACGGCGG

AACTCATCGC

4501

CGCCTGCCTT

GCCCGCTGCT

GGACAGGGGC

TCGGCTGTTG

GGCACTGACA

ATTCCGTGGT

4561

GTTGTCGGGG

AAGCTGACGT

CCTTTCCATG

GCTGCTCGCC

TGTGTTGCCA

CCTGGATTCT

4621

GCGCGGGACG

TCCTTCTGCT

ACGTCCCTTC

GGCCCTCAAT

CCAGCGGACC

TTCCTTCCCG

4681

CGGCCTGCTG

CCGGCTCTGC

GGCCTCTTCC

GCGTCTTCGC

CTTCGCCCTC

AGACGAGTCG

4741

GATCTCCCTT

TGGGCCGCCT

CCCCGCCTGG

AATTCGAGCT

CGGTACCTTT

AAGACCAATG

4801

ACTTACAAGG

CAGCTGTAGA

TCTTAGCCAC

TTTTTAAAAG

AAAAGGGGGG

ACTGGAAGGG

4861

CTAATTCACT

CCCAACGAAG

ACAAGATCTG

CTTTTTGCTT

GTACTGGGTC

TCTCTGGTTA

4921

GACCAGATCT

GAGCCTGGGA

GCTCTCTGGC

TAACTAGGGA

ACCCACTGCT

TAAGCCTCAA

4981

TAAAGCTTGC

CTTGAGTGCT

TCAAGTAGTG

TGTGCCCGTC

TGTTGTGTGA

CTCTGGTAAC

5041

TAGAGATCCC

TCAGACCCTT

TTAGTCAGTG

TGGAAAATCT

CTAGCAGTAG

TAGTTCATGT

5101

CATCTTATTA

TTCAGTATTT

ATAACTTGCA

AAGAAATGAA

TATCAGAGAG

TGAGAGGAAC

5161

TTGTTTATTG

CAGCTTATAA

TGGTTACAAA

TAAAGCAATA

GCATCACAAA

TTTCACAAAT

5221

AAAGCATTTT

TTTCACTGCA

TTCTAGTTGT

GGTTTGTCCA

AACTCATCAA

TGTATCTTAT

5281

CATGTCTGGC

TCTAGCTATC

CCGCCCCTAA

CTCCGCCCAT

CCCGCCCCTA

ACTCCGCCCA

5341

GTTCCGCCCA

TTCTCCGCCC

CATGGCTGAC

TAATTTTTTT

TATTTATGCA

GAGGCCGAGG

5401

CCGCCTCGGC

CTCTGAGCTA

TTCCAGAAGT

AGTGAGGAGG

CTTTTTTGGA

GGCCTAGGGA

5461

CGTACCCAAT

TCGCCCTATA

GTGAGTCGTA

TTACGCGCGC

TCACTGGCCG

TCGTTTTACA

5521

ACGTCGTGAC

TGGGAAAACC

CTGGCGTTAC

CCAACTTAAT

CGCCTTGCAG

CACATCCCCC

5581

TTTCGCCAGC

TGGCGTAATA

GCGAAGAGGC

CCGCACCGAT

CGCCCTTCCC

AACAGTTGCG

5641

CAGCCTGAAT

GGCGAATGGG

ACGCGCCCTG

TAGCGGCGCA

TTAAGCGCGG

CGGGTGTGGT

5701

GGTTACGCGC

AGCGTGACCG

CTACACTTGC

CAGCGCCCTA

GCGCCCGCTC

CTTTCGCTTT

5761

CTTCCCTTCC

TTTCTCGCCA

CGTTCGCCGG

CTTTCCCCGT

CAAGCTCTAA

ATCGGGGGCT

5821

CCCTTTAGGG

TTCCGATTTA

GTGCTTTACG

GCACCTCGAC

CCCAAAAAAC

TTGATTAGGG

5881

TGATGGTTCA

CGTAGTGGGC

CATCGCCCTG

ATAGACGGTT

TTTCGCCCTT

TGACGTTGGA

5941

GTCCACGTTC

TTTAATAGTG

GACTCTTGTT

CCAAACTGGA

ACAACACTCA

ACCCTATCTC

6001

GGTCTATTCT

TTTGATTTAT

AAGGGATTTT

GCCGATTTCG

GCCTATTGGT

TAAAAAATGA

6061

GCTGATTTAA

CAAAAATTTA

ACGCGAATTT

TAACAAAATA

TTAACGCTTA

CAATTTAGGT

6121

GGCACTTTTC

GGGGAAATGT

GCGCGGAACC

CCTATTTGTT

TATTTTTCTA

AATACATTCA

6181

AATATGTATC

CGCTCATGAG

ACAATAACCC

TGATAAATGC

TTCAATAATA

TTGAAAAAGG

6241

AAGAGTATGA

GTATTCAACA

TTTCCGTGTC

GCCCTTATTC

CCTTTTTTGC

GGCATTTTGC

6301

CTTCCTGTTT

TTGCTCACCC

AGAAACGCTG

GTGAAAGTAA

AAGATGCTGA

AGATCAGTTG

6361

GGTGCACGAG

TGGGTTACAT

CGAACTGGAT

CTCAACAGCG

GTAAGATCCT

TGAGAGTTTT

6421

CGCCCCGAAG

AACGTTTTCC

AATGATGAGC

ACTTTTAAAG

TTCTGCTATG

TGGCGCGGTA

6481

TTATCCCGTA

TTGACGCCGG

GCAAGAGCAA

CTCGGTCGCC

GCATACACTA

TTCTCAGAAT

6541

GACTTGGTTG

AGTACTCACC

AGTCACAGAA

AAGCATCTTA

CGGATGGCAT

GACAGTAAGA

6601

GAATTATGCA

GTGCTGCCAT

AACCATGAGT

GATAACACTG

CGGCCAACTT

ACTTCTGACA

6661

ACGATCGGAG

GACCGAAGGA

GCTAACCGCT

TTTTTGCACA

ACATGGGGGA

TCATGTAACT

6721

CGCCTTGATC

GTTGGGAACC

GGAGCTGAAT

GAAGCCATAC

CAAACGACGA

GCGTGACACC

6781

ACGATGCCTG

TAGCAATGGC

AACAACGTTG

CGCAAACTAT

TAACTGGCGA

ACTACTTACT

6841

CTAGCTTCCC

GGCAACAATT

AATAGACTGG

ATGGAGGCGG

ATAAAGTTGC

AGGACCACTT

6901

CTGCGCTCGG

CCCTTCCGGC

TGGCTGGTTT

ATTGCTGATA

AATCTGGAGC

CGGTGAGCGT

6961

GGGTCTCGCG

GTATCATTGC

AGCACTGGGG

CCAGATGGTA

AGCCCTCCCG

TATCGTAGTT

7021

ATCTACACGA

CGGGGAGTCA

GGCAACTATG

GATGAACGAA

ATAGACAGAT

CGCTGAGATA

7081

GGTGCCTCAC

TGATTAAGCA

TTGGTAACTG

TCAGACCAAG

TTTACTCATA

TATACTTTAG

7141

ATTGATTTAA

AACTTCATTT

TTAATTTAAA

AGGATCTAGG

TGAAGATCCT

TTTTGATAAT

7201

CTCATGACCA

AAATCCCTTA

ACGTGAGTTT

TCGTTCCACT

GAGCGTCAGA

CCCCGTAGAA

7261

AAGATCAAAG

GATCTTCTTG

AGATCCTTTT

TTTCTGCGCG

TAATCTGCTG

CTTGCAAACA

7321

AAAAAACCAC

CGCTACCAGC

GGTGGTTTGT

TTGCCGGATC

AAGAGCTACC

AACTCTTTTT

7381

CCGAAGGTAA

CTGGCTTCAG

CAGAGCGCAG

ATACCAAATA

CTGTTCTTCT

AGTGTAGCCG

7441

TAGTTAGGCC

ACCACTTCAA

GAACTCTGTA

GCACCGCCTA

CATACCTCGC

TCTGCTAATC

7501

CTGTTACCAG

TGGCTGCTGC

CAGTGGCGAT

AAGTCGTGTC

TTACCGGGTT

GGACTCAAGA

7561

CGATAGTTAC

CGGATAAGGC

GCAGCGGTCG

GGCTGAACGG

GGGGTTCGTG

CACACAGCCC

7621

AGCTTGGAGC

GAACGACCTA

CACCGAACTG

AGATACCTAC

AGCGTGAGCT

ATGAGAAAGC

7681

GCCACGCTTC

CCGAAGGGAG

AAAGGCGGAC

AGGTATCCGG

TAAGCGGCAG

GGTCGGAACA

7741

GGAGAGCGCA

CGAGGGAGCT

TCCAGGGGGA

AACGCCTGGT

ATCTTTATAG

TCCTGTCGGG

7801

TTTCGCCACC

TCTGACTTGA

GCGTCGATTT

TTGTGATGCT

CGTCAGGGGG

GCGGAGCCTA

7861

TGGAAAAACG

CCAGCAACGC

GGCCTTTTTA

CGGTTCCTGG

CCTTTTGCTG

GCCTTTTGCT

7921

CA

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