名称: | pCMV-Akap13(mouse)-mut-Neo |
---|---|
别称: | SC93367 |
基因长: | 8331bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGAGCTCGA
TGAAACTTAG
TCCACAACAA
GCTCCATTAT
ATGGCGATTG
TGTTGTTACA
GTATTGCTTG
CTGAAGAGGA
CAAAGTTGAA
GATGATGCTA
TTTTTTACTT
GATATTTTCT
GGCTCTACCC
TCTATCACTG
CACAAGTACC
CGGAAAGTCA
GTTCTGATAC
GTTGGAGACA
ATTGCTCCTG
GTCACGACTG
CTGTGAGACA
GTGAAGGTGC
TGCTCTGTGC
TTCCAGAGAG
GGCCTTCCTG
TGTTTGTAGT
CGCTGAAGAA
GACTTCCATT
TCGTCCAGGA
TGAGGCGTAT
GATGCAGCTC
AGTTCCTGGC
AACCAGTGCT
GGAAATCAGC
AGGCTCTGAA
CTTCACACGG
TTCCTCGATC
GATCAGGACC
TCCTTCTAGA
GATGTGAACT
CTCTCGATGA
GAAGGTGGCA
CTGGCATTCA
GACACCTGAA
GCTGCCTGCA
GAATGGAACG
TGTTAGGGAC
AGATCACACT
TTGCATGACG
GTGGCCCTCG
GGAAACCCTG
ATGCACTTTG
CTGTGCGGCT
GGGACTGCTG
CGGTTGACAT
GGTTCCTGTT
ACAGAAGCCA
GGTGGCCGTG
GAGCTCTCAG
CATCCACAAC
AAGGAAGGGG
CAACGCCTGT
GAGCCTGGCA
CTGGAGCGAG
GTTATCACGA
ACTTCACCAG
CTTCTGACTG
AGGAAAATGC
AGGGGAGCCA
GATTCCTGGA
GCAGCTTATC
CTATGAAATC
CCTTATGGCG
ACTGTTCAGT
GAGGCACCAT
CGAGAACTGG
ACATCTACAC
ATTGACTTCT
GAATCCGAGT
CACATCGTGA
ACCCCATGGA
GACAGCTGTA
CTGGACATAT
TTCTAAACTT
ATGAATATCC
AACAGCAGCT
GATGAAAACA
AACTTGAAGC
AGATGGACAA
TCTCATGCCA
TTAATGGTGA
CAGCACAAGA
TTCGTCTTGT
GTGCCCAGTG
TACCAGAGAC
AGATGGCCTA
TTCCTTCCCT
GTGTACCAGA
GCCTTCTGAC
CACCAGCATC
CTCCTTTTGA
AGAAACTAAG
AGCACTCTGT
GCTGCCAAAG
GAGTCCTGGG
AGGATGGCTG
AAAGTTCCTG
TGATTTGTCA
AGCATGGTTG
AGGAAGAAAA
TGTCATCTGT
TCTCATAAGA
AAAATAAAGA
TGTGGGGAGA
AAGGGGGAAG
AGGCGGAGCC
AGCATCTGCC
ATGGACTCCG
GGTCTGCATC
TCATCAAGAC
AGCTGCCTTC
AGAGCGTACC
CGACTGTGGA
GTGAAGGGCA
GAGAAGGTCT
TCCATCCTGT
GGAAACAGAA
ACGAAGTAAC
TGGAACAAAC
TATTCTGGAG
TAGCCACATG
TCAGCAGCCA
CTGAGCAGCA
GGAGCAGTGT
GCTGCAGGAT
GCCATGGTCA
CAGAACCAGA
TGCATGCCAG
CACTCTTCTG
GAAGGGAGCT
ACCAGACAGC
TCTTCAACAG
ATGTTGGTGC
CCCAGAAAAG
GCAGGGGAAC
TGGAACATAG
CCTGCTTACC
CCAGATGCTA
CCACCCAGAA
CAATAAGCCT
CAAGTAGGGG
AAGGCACAAA
GGAAAGACTT
GAGAATTCAG
ACAGTAGCAC
AACTGAAACA
ACTGCTGTTC
AAGTTTTAAG
TGAACCCATG
GAGAAAGCCG
ATATTACAAA
CCATGTCTTT
GCCACCAGTG
CCGTAGGTGT
TAACACTCCT
GCTGAGGCTT
CACCTGCATT
GAGTTCTGAA
GAAATCCCCA
CTGAAAAACC
AGGAATGGAA
ACTCAAGAGA
GAGGCTGTGA
GGGGGGAACC
ACAAGTGACC
AGAGCTCCCC
GGTGCTTCCT
GCTGCTGCTA
TAGAAAACAA
GGTTTTAGGT
GGACAAGAAC
CTGATACTAG
TATAAGCGGC
TTTTGTAAGA
CAGCATCACC
TTTGGATTTA
ACCATGCCAG
GCCCAAGTTC
AGATGGGATG
CCAGAACAGA
ACTCAGAGTC
TCATGCACGG
CCTGCTCAAA
GCCTAAGTAG
CCAGGCGCTG
CTCTGTTCTA
CAGCTGAGGC
TGGCACGCCT
TCTGCTGAGG
CTACACATCA
GCCGAGCACA
GTGACTTCTA
GTGGCAGGTT
GGAAGAATGT
GGCAGTGGTA
AAGCTAGCCT
GCCTGAAAGC
ACAATGGTGC
AGCCAAGCAC
GCAAGAACTT
TGCACCACCC
TCTGCCCAGA
AGACCCACAA
GCTGACACAG
TCACTTCTGA
CACTGTGAAA
AATACCCAGA
AATCTGTGGG
GGTTTGTCAT
CTCTGTGTTT
CAGATGCAAA
GAATCAAGGA
AATGGTTTGA
AGCAAGATAC
ACCTTTAACG
AACGTGCTTG
AGGATGTTCC
ACGTCTGCCT
TCAGTTGTCT
CCCAGACTGA
GAAAGAACTA
GCGCCAGACC
AGGTATCACC
TCCAGGCAGC
AGTTTCTCTC
TGGCAAGCAG
TCCAGAGAGT
GAATCAGTAA
CCAAAGATGA
CGCACTTTCT
CTTGTCCCCT
CCCAGAAAGA
AAAGGGAACA
GCAACTCCTC
AGCTCCATAG
AACTACAGCT
TGTCGAGATG
GCCCAGATGG
AAGAGATTTG
AGTGATACCG
ATAAGGTAGG
AGACGGGGCC
ACTGACCCAC
CTCCATCCTC
TGCTGTAGAG
CTTCGGACCA
GCATGGGAAA
TACCAGCCCT
GTAGGCATTG
GTGGAGAACA
GGAGGGTTCC
AGCCCAACAG
CAACACTTGA
AGTGCTGAGT
GACTCTTTAC
TGCATAACGT
GGATAAAGCT
GCTTTGGTTT
CTGACTTTAC
TCTGCCTGAA
GAAGGTGTCA
GTGTAGTGGG
TCCAGAAAGT
TCCACAGCTC
TGGGGCAAGA
TGGCAAAGAT
AGAGCAATGA
GCTGTTCTTC
AGTTAAAGAA
GACGTGCATT
CTTCAGAAAT
GTCAAGGGAA
GACCAGAGAA
CACCTCCCTC
TGGGCAGGAG
ATACCAGGAC
TCTGTGAAAA
ACCTATGTCA
GCCCTCTGTG
CCGAGGAGAA
AGCACAGCAG
CACACACCCT
CTGCCTGTCT
GAAAACAGAA
ACTAAAGACA
TCAAGGAAGT
GGCCCCACAG
GTCTCCCTGC
TGACTGAAGG
TGGGGCTGCA
AAGAGCCTGG
TGCCACCAAG
AACGAGTCTG
TCTGCCGATT
CAAAGCAGAA
AGCCTCGAGT
ACAGAGCAGA
GCGGCTCATC
TCTGTTGCCA
AGTGGGCTGC
CAGGTGCGTC
TGAGGCACTC
CATTGTAATC
AGCCTTCTGC
TCTCGATGTT
GTCGTAGAGA
ATACTCAGTT
CCAAGGGGAA
ACCAATGCTT
GTGAGGTAAG
CAGAAGTGCG
ATGGAGGATG
TTACAGTGGC
TGATGCTTCA
CCAGCTACTG
CTGAGCCCAG
AAGAAAGGAT
GCATCACACT
GTATCAAAGA
CATCCCAGTT
TCAGAGTTGT
TAAGCCAAGA
GAAGCAAATG
ACTCCAAGTT
TGCCAGAAGC
TTTCCTGGAC
AAAGGTGTCA
CTGACTTACA
GGAGGTCATA
ACCCCAGAGA
TAGAACCACT
TGATTGCAAG
AGAGAGACGC
TGGAGGGAAC
AGACCTTAAC
TGTGCCACGA
GTAACTCCAA
AGAGACCCCA
ATCGAAAAAC
CAATGCAGCC
TCTTGCCAGG
GACCTCCCCA
CAGAGACAGG
GCTCTCAGTG
ATCAATAACA
ATGTCCCCCA
AGCTGACATG
AAGCAAGTGG
CACAGGCTTC
CGTCCCAGCA
GAGGAAAGTA
ATGCCACTAC
TGTCTCAACC
CAAGCTGCAG
ATGTGTCTAC
CAGAGCAGAC
TCCATAGAGG
AGACCGCCAC
TAGGATAGTG
GAGGCCGTCA
TCAGGCAAGT
CAGGGCCTCC
AACACACTGA
TGGCTAAAGT
AGAAACCCAG
AATCCGTCAC
TCTCCAGTCC
AGAGACTAAG
CAGCTAGAGA
ATGCTTACAC
AGAGAGTGCA
TGCGCTTTCC
TGCCTGGGGA
GACCCCGCAG
ATAGAGAAGA
CGCACGAAGA
CACCACCGGG
CAGTGTGGTG
CTGAGACAGA
AGAACCAGAG
AAAATTATTC
TACCTGAGCC
AGCACCAGAA
ATGCCAGACA
CCAGAACTGG
AGATGAAGTG
GACCTTCTGA
GTAGGATCAG
TGCTGCTTCT
GAGGAGGAGG
CTGTAGGAAA
TGGAGCTGCC
ACACCCAAGA
TGAAGCAAGG
CCCGGGGACC
CAGGCGATCA
ACCGAGAAAG
CTGGTGTGCC
ATAGAGCCCT
GCCCTGAAGC
AGCATCTCTC
TTAGCTTCCA
AACAGAGCTC
AGAATGCAGA
AGCTTCATAG
ATGTTGGACT
GGGCACGGAG
TGTGCCTCAA
AAGAAGGCAT
GCTCCAGAGA
GTGTCAGGGA
GTGAGTCTGA
CCTCTTTCAC
TCACCCAGTG
ATGAAATGGA
CAGCATCATC
TTCCCAAAGC
CTGAAGAAGA
GCAGTTACTT
TGTGATACAA
CAGGATCCAG
TTCTTCCACA
GATGACACAG
CTTCCCTGGA
TCGACATTCT
TCTCATGGCA
GTGACGTGTC
CCTTCCTCAG
ACTTCAAAGT
TAAACAGATC
CAGAAATCAT
CAAAGTGCCA
ATGGCTTTTT
CAGCCCTGGA
GTCGAGGCAC
CTGAGAGTCG
AGAGAGTGAG
AGTGAGCCTG
CTGGCTCTGG
TGAAATGGAA
GAGGAGGAGA
TGGACAGCAT
CACTGAAGTG
CCTGCCAACT
GCTCTTTTCT
GAGGAGCTCC
ATGCGTTCTC
TTTCTCCTTT
CCGGAGGCAC
AGCTGGGGAC
CTGGGAAGAA
CGCAGCCAGT
GATGCAGAAA
TGAACCAGCG
GAGTTCAATG
CGAGCTCTTG
GGCATGTTGT
CAGGAGACCT
CCCATTCATA
GGAGAAGTTT
CAGTCTGGAA
GGCTTGACAG
GAGGAGGTGT
TGGAAATAAG
CCATCCTCGT
CTCTAGAAAT
GAGCTCTGCA
AACTCCAGCG
AGCTCAGAAA
CCCTTTCGGT
GGTGAGGAAC
AGAGAAACTC
TTTGATGTCA
CTTTCAGAAG
AACATCTGGA
GCCAGACCAG
AGACAGCATC
ATAGGATGTT
TGATCAGCAG
ACATGTTACA
GGTCTAAACA
ACAGGGATTT
AACTACTGTA
CATCAGCCAT
TTCTTCTCCA
TTGACAAAAT
CCATCTCATT
AATGACAATC
AGTCATCCTG
GATTGGACAG
TTCACGGCCC
TTCCACAGTA
CCAGTGCCAA
CCTGACCGAG
AGTATAACAG
AAGAAAACTG
TAACTTCCTG
CCTCCAAGTC
CCTCAAAAAA
GAATTTTGAA
GAAAAGAGTG
GGACTAAGGT
TAGTCGCACC
TTCAGCTACA
TCAGGAATAA
AATGTCCAGC
AGCAAGAAGA
GCAAAGAAAA
GGAAAAGGAG
AAGGATAAAA
TTAAGGAGAA
AGAGAAAGAC
TCTAAAGAGA
AGGAAAAAGA
TAAAAAGACT
CTTAACGGGC
ACACATTCAG
CCCCATTCCT
ATTGTGGGTC
CCATCAGCTG
CAGCCAGTGC
ATGAAGCCTT
TTACCAACAA
GGACGCCTAC
ACGTGTGCAG
GCTGTGGTGC
TTTTGTCCAC
AAAGGCTGCA
GAGAAAATCT
AGCCTCCTGT
GCAAAGGTCA
AAATGAAGCA
GCCAAAAGGG
AGCCTCCAGG
CCCACGATAC
ATCATCGCTG
CCCACAGTCA
TCATGAGAAA
CAAGTCTTCA
CAGCCCAAGG
AGCGCCCTAG
GTCTGCAGTC
CTCTTGGCTG
ATGAGGCCAC
TGCGGCACCA
ATGTTTACTA
ACAGACGGTC
CCAGCAGAGT
GTCTCCCTCT
CTAAAAGTGT
CTCCATACAA
AACATCACTG
GAGTTGGCAA
TGATGAGAAC
ATGTCAAACA
CCTGGAAATT
TCTATCTCAT
TCAACAGACT
CATTGAATAA
AATCTGCAAG
GTCAATGAGT
CAACAGAATC
CCTTACTGAT
GAGGGAGTAG
GTACAGACAT
GAATGAAGGA
CAGCTGATGG
GAGATTTTGA
AAGCGACTCT
AAACAGCTAG
AAGCAGAGTC
ATGGAGCCGG
ACAGTGGACA
GCAAGTTCCT
TAAACAGCAG
AAGAAAGATG
TGGTCAAACG
GCAGGAAGTG
ATTTACGAGT
TGATGCAGAC
AGAGTTACAT
CACATCCGCA
CACTCAAGAT
CATGAGTGAC
GTGTACAGCC
GGGGCATGAT
GACAGATCTG
CTCTTTGAGC
AGCAGATGGT
GGAAAAGCTG
TTCCCCTGCT
TGGATGAGCT
GATCAGTATC
CACAGCCAGT
TCTTTCAGAG
GATCCTGGAG
CGGAAGAAGG
AGTCTCTTGT
GGATAAGAGT
GAAAAGAACT
TTCTCATCAA
GAGGATAGGG
GATGTGCTTG
TCAGTCAGTT
CTCAGGTGAG
AGTGCAGAGC
GCTTAAAGAA
GACATACGGC
AAGTTCTGTG
GACAGCACAA
CCAGTCTGTC
AACTACTTCA
AAGACCTCTA
CACCAAGGAT
AAGCGTTTCC
AAGCCTTTGT
GAAGAAGAAG
ATGAGCAGTT
CAGTAGTAAG
AAGGCTGGGA
ATTCCAGAGT
GCATTTTACT
TGTAACTCAG
AGGATCACCA
AGTACCCAGT
TTTATTCCAA
AGGATACTGC
AATGTACCAA
AGACAATGAA
GTGGAGCAGG
AAGACCTCAC
TCAGTCCTTG
AGCCTGGTGA
AGGATGTGAT
TGGAGCCGTG
GACAGCAAAG
TGGCAAGTTA
CGAGAAGAAA
GTGCGTCTCG
GTGAGATTTA
CACAAAGACA
GATAGCAAGT
CGATCATGAG
AATGAAGAGT
GGGCAGATGT
TCGCCAAGGA
GGACCTAAGA
CGGAAGAAGC
TGGTGCGCGA
CGGGAGTGTG
TTTCTGAAGA
GCACAACAGG
AAGGCTGAAA
GAGGTTCAAG
CAGTTCTACT
CACTGACATT
TTAGTTTTCC
TTCAAGAAAA
AGACCAGAAA
TACGTGTTTG
CATCCTTGGA
CCATAAGTCA
ACAGTGATCT
CCTTAAAGAA
GCTGATTGTA
AGAGAAGTGG
CCCATGAGGA
GAAAGGCCTG
TTCCTCATCA
GTATGGGGGT
GAAAGATCCA
GAGATGGTGG
AGGTCCATGC
AAGCTCCAGA
GAGGAGCGGA
ATAGCTGGAT
TCAGATCATT
CAGGACACAA
TCAACTCCCT
GAATAGAGAT
GAAGATGAAG
GGATTCCCAG
TGAGAATGAA
GAAGAAAAGA
AATTGTTGGA
TACCAAAGCC
CGGGAGTTAA
AAGAACAGCT
TCAGCAGAAG
GACCAGCAGA
TCCTCCTCTT
GCTGGAAGAG
AAGGAGATGA
TTTTCCGGGA
CATGACTGAG
TGCAGCACTC
CCTTGCCAGA
GGATTGCTCC
CCAACGCACA
GCCCGAGAGT
GCTCTTCCGC
TCCAACACAG
AGGAGGCTCT
CAAAGGAGGA
CCGCTGATGA
AAAGTGCAAT
AAATGAAGTG
GAAATCCTTC
AGAGTTTGGT
GAGTGGAAGC
CTCGGTGGCA
CACTTGGACA
GTCCATCAGC
AGCCCTGTTG
AACAGGAAGT
CATGGCTGCG
CCCATTTCTT
TGCCCCGGAG
AGCAGAGACC
TTTGGAGGAT
TTGACTGTCA
TCAGATGAAT
GCTTCCAAAG
GAGGTGAGAA
AGAAGAGGGT
GATGACGGCC
AAGATCTCAG
GAGAACAGAG
TCAGACAGCG
GCCTGAAAAA
GGGTGGAAAT
GGTAACCTGG
TATTTATGCT
TAAAAGAAAC
AGTGAGCAGG
TTGTCCAGAG
CATTGTTCAC
CTCCACGAAC
TCCTCAGCAT
GCTGCAGGGT
GTCGTGCTAC
AACAAGACAG
CTACATTGAG
GATCAGAAGC
TGGTGCTGAC
AGAGAAGGTC
CTCACAAGGA
GTGCATCTCG
CCCCAGCTCT
CTCATTGAGC
AGGAGAAGCA
GCGGAGCCTG
GAGAAGCAGC
GCCAGGACCT
GGCCAACCTG
CAGAAGCAGC
AAGCACAGCA
CCTGGAGGAA
AAGCGCCGCC
GGGAGCGTGA
GTGGGAGGCC
CGGGAGCAGG
AGCTCCGAGA
CCGAGAGGCC
AAGCTGGCCG
AGCGGGAAGA
GACGGTCCGC
CGCAGGCAGC
AGGACCTGGA
GAGGGACCGC
GAAGAGCTCC
AGCAGAAGAA
GGGCACTTAC
CAGTGTGACC
TAGAGAGGCT
ACGGGCTGCC
CAGAAACAGC
TGGAACGAGA
ACAGGAACAG
CTGAGGAGGG
ACACAGAGCG
GCTCAGCCAG
AGGCAGATGG
ATCAGAACCT
TTGCCAGGTT
TCAAATAAAC
ATGGCAGGCT
GATGCGGATC
CCATCCTTCT
TGCCCAATTC
GGATGAGTTC
TCCTCGCCGT
CTGCACCTTC
TGTAACCAAA
TCAGGCTCAT
TGGACTCAGA
ACTCTCAGTG
TCTCCTAAAA
GGAACAGCAT
CTCTCGGACA
CAGAAAGATA
AGGGGCCTTT
CCATATACTG
GGCTCAGCCA
GCCAGACAAA
AGTTCCCGAG
GGGCAGAGCC
AGGCCCCGTC
CAGCACCTCC
ACTTCCACCC
GACTCTTTGG
GTTATCCAAG
CCAAAGGAAA
AGAAGGAGAA
AAAGAAGAAG
AGCAAAGGAA
GCCGCACCCA
GCCTGGTGAC
GGCCCTGCTT
CGGAAGTACC
AGCAGAGGGT
GAAGAGATCT
TCTGTTGAGA
TATCCAGCAC
AGTGGCGGCC
GCTCGAGTCT
AGAGGGCCCG
TTTAAACCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
TAGGGGGTAT
CCCCACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTAATTCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
AGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCTGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TCCCGGGAGC
TTGTATATCC
ATTTTCGGAT
CTGATCAAGA
GACAGGATGA
GGATCGTTTC
GCATGATTGA
ACAAGATGGA
TTGCACGCAG
GTTCTCCGGC
CGCTTGGGTG
GAGAGGCTAT
TCGGCTATGA
CTGGGCACAA
CAGACAATCG
GCTGCTCTGA
TGCCGCCGTG
TTCCGGCTGT
CAGCGCAGGG
GCGCCCGGTT
CTTTTTGTCA
AGACCGACCT
GTCCGGTGCC
CTGAATGAAC
TGCAGGACGA
GGCAGCGCGG
CTATCGTGGC
TGGCCACGAC
GGGCGTTCCT
TGCGCAGCTG
TGCTCGACGT
TGTCACTGAA
GCGGGAAGGG
ACTGGCTGCT
ATTGGGCGAA
GTGCCGGGGC
AGGATCTCCT
GTCATCTCAC
CTTGCTCCTG
CCGAGAAAGT
ATCCATCATG
GCTGATGCAA
TGCGGCGGCT
GCATACGCTT
GATCCGGCTA
CCTGCCCATT
CGACCACCAA
GCGAAACATC
GCATCGAGCG
AGCACGTACT
CGGATGGAAG
CCGGTCTTGT
CGATCAGGAT
GATCTGGACG
AAGAGCATCA
GGGGCTCGCG
CCAGCCGAAC
TGTTCGCCAG
GCTCAAGGCG
CGCATGCCCG
ACGGCGAGGA
TCTCGTCGTG
ACCCATGGCG
ATGCCTGCTT
GCCGAATATC
ATGGTGGAAA
ATGGCCGCTT
TTCTGGATTC
ATCGACTGTG
GCCGGCTGGG
TGTGGCGGAC
CGCTATCAGG
ACATAGCGTT
GGCTACCCGT
GATATTGCTG
AAGAGCTTGG
CGGCGAATGG
GCTGACCGCT
TCCTCGTGCT
TTACGGTATC
GCCGCTCCCG
ATTCGCAGCG
CATCGCCTTC
TATCGCCTTC
TTGACGAGTT
CTTCTGAGCG
GGACTCTGGG
GTTCGAAATG
ACCGACCAAG
CGACGCCCAA
CCTGCCATCA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GTATACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTC