名称: | pLV-Tet-miniCMV-KLHL24(human)-GFP-nanobody-mCherry-Puro |
---|---|
别称: | NM_001349413.1;CMH29; DRE1; EBS6; EBSSH; KRIP6 |
基因长: | 1875bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 红色 |
启动子: | Tet-miniCMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV-Tet-miniCMV-MCS-GFP-nanobody-mCherry-Puro |
正向引物: | PFLAG-CMV-F |
反向引物: | |
诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTTCCACT
GTGGCAGTGA
ACTCCAAGCC
ACCATGGTAC
TAATATTGGG
ACGCAGACTA
AACAGAGAGG
ATCTTGGGGT
GCGTGATTCC
CCAGCAACTA
AGCGAAAAGT
TTTTGAAATG
GACCCCAAAT
CTCTGACAGG
TCATGAGTTT
TTTGACTTCT
CTTCAGGATC
ATCCCATGCC
GAAAACATAC
TCCAGATATT
TAATGAATTT
CGTGATAGCC
GCTTATTCAC
AGATGTTATC
ATTTGTGTGG
AAGGAAAAGA
ATTTCCTTGC
CATAGAGCTG
TGCTCTCAGC
CTGTAGCAGC
TACTTCAGAG
CTATGTTTTG
TAATGACCAC
AGGGAAAGCC
GAGAAATGTT
GGTTGAGATC
AATGGTATTT
TAGCTGAAGC
TATGGAATGT
TTTTTGCAGT
ATGTTTATAC
TGGAAAGGTG
AAGATCACTA
CAGAGAATGT
ACAGTATCTC
TTTGAGACAT
CAAGCCTCTT
TCAGATTAGT
GTTCTCCGTG
ATGCATGTGC
CAAGTTCTTG
GAGGAGCAAC
TTGATCCTTG
TAATTGCTTA
GGAATCCAGC
GCTTTGCTGA
TACCCATTCA
CTCAAAACAC
TCTTCACAAA
ATGCAAAAAT
TTTGCGTTAC
AGACTTTTGA
GGATGTATCC
CAGCACGAAG
AATTTCTTGA
GCTTGACAAA
GATGAACTTA
TTGATTATAT
TTGTAGTGAT
GAACTTGTTA
TTGGTAAAGA
GGAGATGGTT
TTTGAAGCCG
TCATGCGTTG
GGTCTATCGT
GCCGTTGATC
TGAGAAGACC
ACTGTTACAC
GAGCTCCTGA
CACATGTGAG
ACTCCCTCTG
TTGCATCCCA
ACTACTTTGT
TCAAACAGTT
GAAGTGGACC
AATTGATCCA
GAATTCTCCT
GAGTGTTATC
AGTTGTTGCA
TGAAGCAAGA
CGGTACCACA
TACTTGGGAA
TGAAATGATG
TCCCCAAGGA
CTAGGCCACG
CAGGTCCACT
GGCTATTCTG
AGGTGATAGT
TGTCGTTGGA
GGATGTGAGC
GAGTTGGAGG
ATTTAATCTT
CCATACACTG
AGTGCTACGA
TCCTGTAACA
GGAGAATGGA
AGTCTTTGGC
TAAGCTTCCA
GAATTTACCA
AATCAGAGTA
TGCAGTCTGT
GCTCTAAGGA
ATGACATTCT
TGTTTCAGGT
GGAAGAATCA
ACAGCCGTGA
TGTCTGGATT
TATAACTCAC
AGTTAAATAT
TTGGATCAGA
GTTGCCTCTC
TCAATAAAGG
CAGATGGCGT
CACAAAATGG
CTGTCCTCCT
TGGTAAAGGG
TTTCTTAAAA
CTTCTATACT
AGGCCAGTCG
TGGTGGCTCA
CACCTGTAAT
CCCAGCACTT
TGGGAGGCCA
AGGTATATGT
TGTCGGTGGC
TATGATGGGC
AAAACAGACT
TAGCAGCGTA
GAATGTTATG
ATTCCTTTTC
AAATCGATGG
ACTGAAGTTG
CTCCCCTTAA
GGAAGCCGTG
AGTTCTCCTG
CAGTGACTAG
CTGTGTAGGC
AAACTGTTTG
TGATTGGTGG
AGGACCTGAT
GATAATACTT
GTTCTGATAA
GGTTCAATCT
TATGATCCAG
AAACCAATTC
TTGGCTACTT
CGTGCAGCTA
TCCCAATTGC
CAAAAGGTGT
ATAACAGCTG
TATCCCTAAA
CAACCTGATC
TATGTTGCCG
GTGGACTGAC
CAAGGCAATA
TACTGTTACG
ATCCAGTTGA
AGATTACTGG
ATGCACGTAC
AGAATACATT
CAGCCGTCAG
GAAAACTGTG
GTATGTCTGT
GTGTAATGGT
AAAATATATA
TCCTGGGCGG
AAGACGGGAA
AATGGAGAAG
CCACAGACAC
TATTCTCTGT
TATGATCCTG
CAACAAGTAT
CATCACAGGG
GTAGCTGCAA
TGCCCAGGCC
AGTGTCCTAT
CATGGCTGTG
TGACTATTCA
TAGATACAAT
GAGAAATGCT
TTAAACTCTA
CCCCTACGAC
GTGCCTGACT
ACGCCGGCGG
CGGTGGTTCA
GGCGGTGGCG
GTTCCGGTGG
TGGGGGATCA
GTCCAACTGG
TGGAGTCTGG
TGGCGCTTTG
GTGCAGCCAG
GTGGCTCTCT
GCGTTTGTCC
TGTGCCGCTT
CTGGCTTCCC
AGTGAACCGC
TATTCCATGC
GCTGGTATCG
CCAGGCTCCA
GGCAAAGAGC
GTGAGTGGGT
AGCCGGTATG
TCCAGCGCGG
GTGATCGTAG
CTCCTATGAA
GACTCCGTGA
AGGGCCGTTT
CACCATCAGC
CGTGACGATG
CCCGTAACAC
GGTGTATCTG
CAAATGAACA
GCTTGAAACC
TGAAGATACG
GCCGTGTATT
ACTGTAATGT
GAACGTGGGC
TTCGAGTATT
GGGGCCAAGG
CACCCAGGTC
ACCGTCTCCA
GCTAAGATCC
TTGACTTGCG
GCCGCAACTC
CCACCTGCAA
CATGCGTGAC
TGACTGAGGC
CGCGACTCTA
GAGTCGACCT
GCATCTAGGC
GCCGGAATTA
GATCTCTCGA
GGTTAACGAA
TTCTACCGGG
TAGGGGAGGC
GCTTTTCCCA
AGGCAGTCTG
GAGCATGCGC
TTTAGCAGCC
CCGCTGGGCA
CTTGGCGCTA
CACAAGTGGC
CTCTGGCCTC
GCACACATTC
CACATCCACC
GGTAGGCGCC
AACCGGCTCC
GTTCTTTGGT
GGCCCCTTCG
CGCCACCTTC
TACTCCTCCC
CTAGTCAGGA
AGTTCCCCCC
CGCCCCGCAG
CTCGCGTCGT
GCAGGACGTG
ACAAATGGAA
GTAGCACGTC
TCACTAGTCT
CGTGCAGATG
GACAGCACCG
CTGAGCAATG
GAAGCGGGTA
GGCCTTTGGG
GCAGCGGCCA
ATAGCAGCTT
TGCTCCTTCG
CTTTCTGGGC
TCAGAGGCTG
GGAAGGGGTG
GGTCCGGGGG
CGGGCTCAGG
GGCGGGCTCA
GGGGCGGGGC
GGGCGCCCGA
AGGTCCTCCG
GAGGCCCGGC
ATTCTGCACG
CTTCAAAAGC
GCACGTCTGC
CGCGCTGTTC
TCCTCTTCCT
CATCTCCGGG
CCTTTCGACC
TGCAGCCCAA
GCTTACCATG
GTGAGCAAGG
GCGAGGAGGA
TAACATGGCC
ATCATCAAGG
AGTTCATGCG
CTTCAAGGTG
CACATGGAGG
GCTCCGTGAA
CGGCCACGAG
TTCGAGATCG
AGGGCGAGGG
CGAGGGCCGC
CCCTACGAGG
GCACCCAGAC
CGCCAAGCTG
AAGGTGACCA
AGGGTGGCCC
CCTGCCCTTC
GCCTGGGACA
TCCTGTCCCC
TCAGTTCATG
TACGGCTCCA
AGGCCTACGT
GAAGCACCCC
GCCGACATCC
CCGACTACTT
GAAGCTGTCC
TTCCCCGAGG
GCTTCAAGTG
GGAGCGCGTG
ATGAACTTCG
AGGACGGCGG
CGTGGTGACC
GTGACCCAGG
ACTCCTCCCT
GCAGGACGGC
GAGTTCATCT
ACAAGGTGAA
GCTGCGCGGC
ACCAACTTCC
CCTCCGACGG
CCCCGTAATG
CAGAAGAAGA
CCATGGGCTG
GGAGGCCTCC
TCCGAGCGGA
TGTACCCCGA
GGACGGCGCC
CTGAAGGGCG
AGATCAAGCA
GAGGCTGAAG
CTGAAGGACG
GCGGCCACTA
CGACGCTGAG
GTCAAGACCA
CCTACAAGGC
CAAGAAGCCC
GTGCAGCTGC
CCGGCGCCTA
CAACGTCAAC
ATCAAGTTGG
ACATCACCTC
CCACAACGAG
GACTACACCA
TCGTGGAACA
GTACGAACGC
GCCGAGGGCC
GCCACTCCAC
CGGCGGCATG
GACGAGCTGT
ACAAGGCAAC
AAACTTCTCT
CTGCTGAAAC
AAGCCGGAGA
TGTCGAAGAG
AATCCTGGAC
CGATGACCGA
GTACAAGCCC
ACGGTGCGCC
TCGCCACCCG
CGACGACGTC
CCCAGGGCCG
TACGCACCCT
CGCCGCCGCG
TTCGCCGACT
ACCCCGCCAC
GCGCCACACC
GTCGATCCGG
ACCGCCACAT
CGAGCGGGTC
ACCGAGCTGC
AAGAACTCTT
CCTCACGCGC
GTCGGGCTCG
ACATCGGCAA
GGTGTGGGTC
GCGGACGACG
GCGCCGCGGT
GGCGGTCTGG
ACCACGCCGG
AGAGCGTCGA
AGCGGGGGCG
GTGTTCGCCG
AGATCGGCCC
GCGCATGGCC
GAGTTGAGCG
GTTCCCGGCT
GGCCGCGCAG
CAACAGATGG
AAGGCCTCCT
GGCGCCGCAC
CGGCCCAAGG
AGCCCGCGTG
GTTCCTGGCC
ACCGTCGGCG
TCTCGCCCGA
CCACCAGGGC
AAGGGTCTGG
GCAGCGCCGT
CGTGCTCCCC
GGAGTGGAGG
CGGCCGAGCG
CGCCGGGGTG
CCCGCCTTCC
TGGAGACCTC
CGCGCCCCGC
AACCTCCCCT
TCTACGAGCG
GCTCGGCTTC
ACCGTCACCG
CCGACGTCGA
GGTGCCCGAA
GGACCGCGCA
CCTGGTGCAT
GACCCGCAAG
CCCGGTGCCT
GATCTTATCG
ATAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAATC
ATCGTCCTTT
CCTTGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CATCGATACC
GTCGACCAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGCA
TCTAGAATTA
ATTCCGTGTA
TTCTATAGTG
TCACCTAAAT
CGTATGTGTA
TGATACATAA
GGTTATGTAT
TAATTGTAGC
CGCGTTCTAA
CGACAATATG
TACAAGCCTA
ATTGTGTAGC
ATCTGGCTTA
CTGAAGCAGA
CCCTATCATC
TCTCTCGTAA
ACTGCCGTCA
GAGTCGGTTT
GGTTGGACGA
ACCTTCTGAG
TTTCTGGTAA
CGCCGTCCCG
CACCCGGAAA
TGGTCAGCGA
ACCAATCAGC
AGGGTCATCG
CTAGCCAGAT
CCTCTACGCC
GGACGCATCG
TGGCCGGCAT
CACCGGCGCC
ACAGGTGCGG
TTGCTGGCGC
CTATATCGCC
GACATCACCG
ATGGGGAAGA
TCGGGCTCGC
CACTTCGGGC
TCATGAGCGC
TTGTTTCGGC
GTGGGTATGG
TGGCAGGCCC
CGTGGCCGGG
GGACTGTTGG
GCGCCATCTC
CTTGCATGCA
CCATTCCTTG
CGGCGGCGGT
GCTCAACGGC
CTCAACCTAC
TACTGGGCTG
CTTCCTAATG
CAGGAGTCGC
ATAAGGGAGA
GCGTCGAATG
GTGCACTCTC
AGTACAATCT
AGCTCTGATG
CCGCATAGTT
AAGCCAGCCC
CGACACCCGC
CAACACCCGC
TGACGCGCCC
TGACGGGCTT
GTCTGCTCCC
GGCATCCGCT
TACAGACAAG
CTGTGACCGT
CTCCGGGAGC
TGCATGTGTC
AGAGGTTTTC
ACCGTCATCA
CCGAAACGCG
CGAGACGAAA
GGGCCTCGTG
ATACGCCTAT
TTTTATAGGT
TAATGTCATG
ATAATAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAGAGCGCC
CAATACGCAA
ACCGCCTCTC
CCCGCGCGTT
GGCCGATTCA
TTAATGCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTTGGACAC
AAGACAGGCT
TGCGAGATAT
GTTTGAGAAT
ACCACTTTAT
CCCGCGTCAG
GGAGAGGCAG
TGCGTAAAAA
GACGCGGACT
CATGTGAAAT
ACTGGTTTTT
AGTGCGCCAG
ATCTCTATAA
TCTCGCGCAA
CCTATTTTCC
CCTCGAACAC
TTTTTAAGCC
GTAGATAAAC
AGGCTGGGAC
ACTTCACATG
AGCGAAAAAT
ACATCGTCAC
CTGGGACATG
TTGCAGATCC
ATGCACGTAA
ACTCGCAAGC
CGACTGATGC
CTTCTGAACA
ATGGAAAGGC
ATTATTGCCG
TAAGCCGTGG
CGGTCTGTAC
CGGGTGCGTT
ACTGGCGCGT
GAACTGGGTA
TTCGTCATGT
CGATACCGTT
TGTATTTCCA
GCTACGATCA
CGACAACCAG
CGCGAGCTTA
AAGTGCTGAA
ACGCGCAGAA
GGCGATGGCG
AAGGCTTCAT
CGTTATTGAT
GACCTGGTGG
ATACCGGTGG
TACTGCGGTT
GCGATTCGTG
AAATGTATCC
AAAAGCGCAC
TTTGTCACCA
TCTTCGCAAA
ACCGGCTGGT
CGTCCGCTGG
TTGATGACTA
TGTTGTTGAT
ATCCCGCAAG
ATACCTGGAT
TGAACAGCCG
TGGGATATGG
GCGTCGTATT
CGTCCCGCCA
ATCTCCGGTC
GCTAATCTTT
TCAACGCCTG
GCACTGCCGG
GCGTTGTTCT
TTTTAACTTC
AGGCGGGTTA
CAATAGTTTC
CAGTAAGTAT
TCTGGAGGCT
GCATCCATGA
CACAGGCAAA
CCTGAGCGAA
ACCCTGTTCA
AACCCCGCTT
TAAACATCCT
GAAACCTCGA
CGCTAGTCCG
CCGCTTTAAT
CACGGCGCAC
AACCGCCTGT
GCAGTCGGCC
CTTGATGGTA
AAACCATCCC
TCACTGGTAT
CGCATGATTA
ACCGTCTGAT
GTGGATCTGG
CGCGGCATTG
ACCCACGCGA
AATCCTCGAC
GTCCAGGCAC
GTATTGTGAT
GAGCGATGCC
GAACGTACCG
ACGATGATTT
ATACGATACG
GTGATTGGCT
ACCGTGGCGG
CAACTGGATT
TATGAGTGGG
CCCCGGATCT
TTGTGAAGGA
ACCTTACTTC
TGTGGTGTGA
CATAATTGGA
CAAACTACCT
ACAGAGATTT
AAAGCTCTAA
GGTAAATATA
AAATTTTTAA
GTGTATAATG
TGTTAAACTA
CTGATTCTAA
TTGTTTGTGT
ATTTTAGATT
CCAACCTATG
GAACTGATGA
ATGGGAGCAG
TGGTGGAATG
CCTTTAATGA
GGAAAACCTG
TTTTGCTCAG
AAGAAATGCC
ATCTAGTGAT
GATGAGGCTA
CTGCTGACTC
TCAACATTCT
ACTCCTCCAA
AAAAGAAGAG
AAAGGTAGAA
GACCCCAAGG
ACTTTCCTTC
AGAATTGCTA
AGTTTTTTGA
GTCATGCTGT
GTTTAGTAAT
AGAACTCTTG
CTTGCTTTGC
TATTTACACC
ACAAAGGAAA
AAGCTGCACT
GCTATACAAG
AAAATTATGG
AAAAATATTC
TGTAACCTTT
ATAAGTAGGC
ATAACAGTTA
TAATCATAAC
ATACTGTTTT
TTCTTACTCC
ACACAGGCAT
AGAGTGTCTG
CTATTAATAA
CTATGCTCAA
AAATTGTGTA
CCTTTAGCTT
TTTAATTTGT
AAAGGGGTTA
ATAAGGAATA
TTTGATGTAT
AGTGCCTTGA
CTAGAGATCA
TAATCAGCCA
TACCACATTT
GTAGAGGTTT
TACTTGCTTT
AAAAAACCTC
CCACACCTCC
CCCTGAACCT
GAAACATAAA
ATGAATGCAA
TTGTTGTTGT
TAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGATCAACT
GGATAACTCA
AGCTAACCAA
AATCATCCCA
AACTTCCCAC
CCCATACCCT
ATTACCACTG
CCAATTACCT
AGTGGTTTCA
TTTACTCTAA
ACCTGTGATT
CCTCTGAATT
ATTTTCATTT
TAAAGAAATT
GTATTTGTTA
AATATGTACT
ACAAACTTAG
TAGTTGGAAG
GGCTAATTCA
CTCCCAAAGA
AGACAAGATA
TCCTTGATCT
GTGGATCTAC
CACACACAAG
GCTACTTCCC
TGATTAGCAG
AACTACACAC
CAGGGCCAGG
GGTCAGATAT
CCACTGACCT
TTGGATGGTG
CTACAAGCTA
GTACCAGTTG
AGCCAGATAA
GGTAGAAGAG
GCCAATAAAG
GAGAGAACAC
CAGCTTGTTA
CACCCTGTGA
GCCTGCATGG
GATGGATGAC
CCGGAGAGAG
AAGTGTTAGA
GTGGAGGTTT
GACAGCCGCC
TAGCATTTCA
TCACGTGGCC
CGAGAGCTGC
ATCCGGAGTA
CTTCAAGAAC
TGCTGATATC
GAGCTTGCTA
CAAGGGACTT
TCCGCTGGGG
ACTTTCCAGG
GAGGCGTGGC
CTGGGCGGGA
CTGGGGAGTG
GCGAGCCCTC
AGATCCTGCA
TATAAGCAGC
TGCTTTTTGC
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
GGCGCCCGAA
CAGGGACTTG
AAAGCGAAAG
GGAAACCAGA
GGAGCTCTCT
CGACGCAGGA
CTCGGCTTGC
TGAAGCGCGC
ACGGCAAGAG
GCGAGGGGCG
GCGACTGGTG
AGTACGCCAA
AAATTTTGAC
TAGCGGAGGC
TAGAAGGAGA
GAGATGGGTG
CGAGAGCGTC
AGTATTAAGC
GGGGGAGAAT
TAGATCGCGA
TGGGAAAAAA
TTCGGTTAAG
GCCAGGGGGA
AAGAAAAAAT
ATAAATTAAA
ACATATAGTA
TGGGCAAGCA
GGGAGCTAGA
ACGATTCGCA
GTTAATCCTG
GCCTGTTAGA
AACATCAGAA
GGCTGTAGAC
AAATACTGGG
ACAGCTACAA
CCATCCCTTC
AGACAGGATC
AGAAGAACTT
AGATCATTAT
ATAATACAGT
AGCAACCCTC
TATTGTGTGC
ATCAAAGGAT
AGAGATAAAA
GACACCAAGG
AAGCTTTAGA
CAAGATAGAG
GAAGAGCAAA
ACAAAAGTAA
GACCACCGCA
CAGCAAGCGG
CCGGTGATCT
TCAGACCTGG
ACGATATATA
TGAGGGACAA
TTGGAGAAGT
GAATTATATA
AATATAAAGT
AGTAAAAATT
GAACCATTAG
GAGTAGCACC
CACCAAGGCA
AAGAGAAGAG
TGGTGCAGAG
AGAAAAAAGA
GCAGTGGGAA
TAGGAGCTTT
GTTCCTTGGG
TTCTTGGGAG
CAGCAGGAAG
CACTATGGGC
GCAGCGTCAA
TGACGCTGAC
GGTACAGGCC
AGACAATTAT
TGTCTGGTAT
AGTGCAGCAG
CAGAACAATT
TGCTGAGGGC
TATTGAGGCG
CAACAGCATC
TGTTGCAACT
CACAGTCTGG
GGCATCAAGC
AGCTCCAGGC
AAGAATCCTG
GCTGTGGAAA
GATACCTAAA
GGATCAACAG
CTCCTGGGGA
TTTGGGGTTG
CTCTGGAAAA
CTCATTTGCA
CCACTGCTGT
GCCTTGGAAT
GCTAGTTGGA
GTAATAAATC
TCTGGAACAG
ATTTGGAATC
ACACGACCTG
GATGGAGTGG
GACAGAGAAA
TTAACAATTA
CACAAGCTTA
ATACACTCCT
TAATTGAAGA
ATCGCAAAAC
CAGCAAGAAA
AGAATGAACA
AGAATTATTG
GAATTAGATA
AATGGGCAAG
TTTGTGGAAT
TGGTTTAACA
TAACAAATTG
GCTGTGGTAT
ATAAAATTAT
TCATAATGAT
AGTAGGAGGC
TTGGTAGGTT
TAAGAATAGT
TTTTGCTGTA
CTTTCTATAG
TGAATAGAGT
TAGGCAGGGA
TATTCACCAT
TATCGTTTCA
GACCCACCTC
CCAACCCCGA
GGGGACCCGA
CAGGCCCGAA
GGAATAGAAG
AAGAAGGTGG
AGAGAGAGAC
AGAGACAGAT
CCATTCGATT
AGTGAACGGA
TCTCGACGGT
CG