名称: | pLV-Tet-miniCMV-ASB15(human)-GFP-nanobody-mCherry-Puro |
---|---|
别称: | NM_080928.4 |
基因长: | 1764bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 红色 |
启动子: | Tet-miniCMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV-Tet-miniCMV-MCS-GFP-nanobody-mCherry-Puro |
正向引物: | PFLAG-CMV-F |
反向引物: | |
诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTAGGGCC
GCGGCAGTGA
ACTCCAAGCC
ACCATGGATA
CTAATGATGA
CCCTGATGAA
GACCATCTTA
CAAGTTATGA
TATTCAGCTA
AGTATTCAAG
AATCCATTGA
AGCCAGCAAG
ACTGCACTTT
GTCCTGAAAG
ATTTGTACCC
CTAAGTGCTC
AAAACAGAAA
ACTTGTGGAG
GCCATAAAAC
AAGGTCACAT
TCCTGAGCTC
CAGGAGTATG
TAAAATATAA
ATATGCAATG
GATGAAGCTG
ATGAAAAAGG
ATGGTTTCCA
TTGCATGAAG
CTGTTGTTCA
ACCCATTCAA
CAAATACTTG
AGATTGTTCT
GGATGCATCC
TATAAGACAC
TCTGGGAATT
CAAGACCTGT
GATGGAGAAA
CACCCTTGAC
TTTGGCAGTC
AAAGCTGGTC
TGGTGGAAAA
TGTAAGAACT
TTATTAGAAA
AGGGAGTGTG
GCCCAACACA
AAAAATGATA
AAGGAGAGAC
CCCCCTTCTG
ATTGCTGTGA
AAAAGGGCTC
CTATGACATG
GTGTCGACTC
TGATCAAACA
TAACACTAGC
CTAGACCAGC
CCTGTGTCAA
GCGATGGTCA
GCAATGCATG
AAGCAGCCAA
GCAAGGCCGA
AAAGATATCG
TAGCTCTGCT
GCTGAAACAT
GGAGGCAATG
TCCACCTGAG
AGATGGATTT
GGAGTCACAC
CACTAGGCGT
CGCTGCCGAG
TATGGTCACT
GTGACGTGTT
AGAACATCTA
ATCCACAAAG
GTGGTGATGT
GCTTGCTTTG
GCGGATGATG
GGGCGTCGGT
GCTGTTTGAG
GCAGCAGGAG
GTGGCAATCC
CGACTGCATT
TCCCTCCTGC
TGGAATATGG
AGGAAGCGGA
AATGTACCTA
ACCGAGCAGG
ACATCTTCCT
ATACACCGAG
CTGCCTATGA
GGGGCATTAT
CTTGCACTGA
AATATCTTAT
CCCAGTAACA
TCTAAAAATG
CAATTCGGAA
AAGTGGGCTA
ACACCAATTC
ACTCAGCAGC
AGATGGACAA
AATGCACAGT
GTCTAGAACT
GCTCATTGAA
AATGGTTTTG
ATGTCAACAC
TCTACTTGCT
GACCACATTT
CCCAGAGCTA
TGACGATGAG
AGGAAGACTG
CGCTGTATTT
TGGCGTTTCT
AATAATGACG
TTCATTGCAC
AGAAGTCCTT
CTGGCTGCAG
GTGCAGACCC
AAACTTAGAT
CCCCTCAACT
GTCTACTTGT
TGCAGTGAGG
GCCAATAATT
ATGAAATTGT
CAGGCTGCTT
CTCTCCCATG
GAGCTAATGT
CAATTGTTAT
TTTATGCATG
TGAATGACAC
TCGTTTCCCC
AGTGTCATTC
AATATGCTCT
AAACGACGAG
GTAATGCTGA
GGCTATTGCT
GAATAATGGC
TATCAAGTGG
AGATGTGCTT
TGACTGCATG
CATGGTGACA
TCTTTGGAAA
TTCATTTGTG
TGGTCAGAGA
TACAGGAAGA
GGTGCTGCCA
GGATGGACAT
CTTGTGTAAT
AAAAGATAAC
CCGTTCTGTG
AGTTTATTAC
AGTTCCTTGG
ATGAAGCACT
TGGTAGGCAG
AGTTACTCGT
GTACTAATAG
ATTACATGGA
TTATGTTCCT
CTGTGTGCTA
AACTGAAGTC
TGCACTAGAA
GTACAGAGAG
AATGGCCAGA
AATCCGCCAA
ATACTAGAGA
ATCCTTGTTC
ATTGAAGCAT
TTGTGTCGGT
TAAAAATTCG
AAGGCTTATG
GGTCTCCAGA
AACTCTGCCA
GCCAGCCTCA
GTGGAGAAGC
TTCCTCTACC
ACCAGCTATT
CAAAGATACA
TATTATTTAA
AGAGTATGAT
CTCTATGGAC
AAGAGCTAAA
ATTGACATAC
CCCTACGACG
TGCCTGACTA
CGCCGGCGGC
GGTGGTTCAG
GCGGTGGCGG
TTCCGGTGGT
GGGGGATCAG
TCCAACTGGT
GGAGTCTGGT
GGCGCTTTGG
TGCAGCCAGG
TGGCTCTCTG
CGTTTGTCCT
GTGCCGCTTC
TGGCTTCCCA
GTGAACCGCT
ATTCCATGCG
CTGGTATCGC
CAGGCTCCAG
GCAAAGAGCG
TGAGTGGGTA
GCCGGTATGT
CCAGCGCGGG
TGATCGTAGC
TCCTATGAAG
ACTCCGTGAA
GGGCCGTTTC
ACCATCAGCC
GTGACGATGC
CCGTAACACG
GTGTATCTGC
AAATGAACAG
CTTGAAACCT
GAAGATACGG
CCGTGTATTA
CTGTAATGTG
AACGTGGGCT
TCGAGTATTG
GGGCCAAGGC
ACCCAGGTCA
CCGTCTCCAG
CTAAGATCCT
TGACTTGCGG
CCGCAACTCC
CACCTGCAAC
ATGCGTGACT
GACTGAGGCC
GCGACTCTAG
AGTCGACCTG
CATCTAGGCG
CCGGAATTAG
ATCTCTCGAG
GTTAACGAAT
TCTACCGGGT
AGGGGAGGCG
CTTTTCCCAA
GGCAGTCTGG
AGCATGCGCT
TTAGCAGCCC
CGCTGGGCAC
TTGGCGCTAC
ACAAGTGGCC
TCTGGCCTCG
CACACATTCC
ACATCCACCG
GTAGGCGCCA
ACCGGCTCCG
TTCTTTGGTG
GCCCCTTCGC
GCCACCTTCT
ACTCCTCCCC
TAGTCAGGAA
GTTCCCCCCC
GCCCCGCAGC
TCGCGTCGTG
CAGGACGTGA
CAAATGGAAG
TAGCACGTCT
CACTAGTCTC
GTGCAGATGG
ACAGCACCGC
TGAGCAATGG
AAGCGGGTAG
GCCTTTGGGG
CAGCGGCCAA
TAGCAGCTTT
GCTCCTTCGC
TTTCTGGGCT
CAGAGGCTGG
GAAGGGGTGG
GTCCGGGGGC
GGGCTCAGGG
GCGGGCTCAG
GGGCGGGGCG
GGCGCCCGAA
GGTCCTCCGG
AGGCCCGGCA
TTCTGCACGC
TTCAAAAGCG
CACGTCTGCC
GCGCTGTTCT
CCTCTTCCTC
ATCTCCGGGC
CTTTCGACCT
GCAGCCCAAG
CTTACCATGG
TGAGCAAGGG
CGAGGAGGAT
AACATGGCCA
TCATCAAGGA
GTTCATGCGC
TTCAAGGTGC
ACATGGAGGG
CTCCGTGAAC
GGCCACGAGT
TCGAGATCGA
GGGCGAGGGC
GAGGGCCGCC
CCTACGAGGG
CACCCAGACC
GCCAAGCTGA
AGGTGACCAA
GGGTGGCCCC
CTGCCCTTCG
CCTGGGACAT
CCTGTCCCCT
CAGTTCATGT
ACGGCTCCAA
GGCCTACGTG
AAGCACCCCG
CCGACATCCC
CGACTACTTG
AAGCTGTCCT
TCCCCGAGGG
CTTCAAGTGG
GAGCGCGTGA
TGAACTTCGA
GGACGGCGGC
GTGGTGACCG
TGACCCAGGA
CTCCTCCCTG
CAGGACGGCG
AGTTCATCTA
CAAGGTGAAG
CTGCGCGGCA
CCAACTTCCC
CTCCGACGGC
CCCGTAATGC
AGAAGAAGAC
CATGGGCTGG
GAGGCCTCCT
CCGAGCGGAT
GTACCCCGAG
GACGGCGCCC
TGAAGGGCGA
GATCAAGCAG
AGGCTGAAGC
TGAAGGACGG
CGGCCACTAC
GACGCTGAGG
TCAAGACCAC
CTACAAGGCC
AAGAAGCCCG
TGCAGCTGCC
CGGCGCCTAC
AACGTCAACA
TCAAGTTGGA
CATCACCTCC
CACAACGAGG
ACTACACCAT
CGTGGAACAG
TACGAACGCG
CCGAGGGCCG
CCACTCCACC
GGCGGCATGG
ACGAGCTGTA
CAAGGCAACA
AACTTCTCTC
TGCTGAAACA
AGCCGGAGAT
GTCGAAGAGA
ATCCTGGACC
GATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCAGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGATCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGCGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
ATCTTATCGA
TAATCAACCT
CTGGATTACA
AAATTTGTGA
AAGATTGACT
GGTATTCTTA
ACTATGTTGC
TCCTTTTACG
CTATGTGGAT
ACGCTGCTTT
AATGCCTTTG
TATCATGCTA
TTGCTTCCCG
TATGGCTTTC
ATTTTCTCCT
CCTTGTATAA
ATCCTGGTTG
CTGTCTCTTT
ATGAGGAGTT
GTGGCCCGTT
GTCAGGCAAC
GTGGCGTGGT
GTGCACTGTG
TTTGCTGACG
CAACCCCCAC
TGGTTGGGGC
ATTGCCACCA
CCTGTCAGCT
CCTTTCCGGG
ACTTTCGCTT
TCCCCCTCCC
TATTGCCACG
GCGGAACTCA
TCGCCGCCTG
CCTTGCCCGC
TGCTGGACAG
GGGCTCGGCT
GTTGGGCACT
GACAATTCCG
TGGTGTTGTC
GGGGAAATCA
TCGTCCTTTC
CTTGGCTGCT
CGCCTGTGTT
GCCACCTGGA
TTCTGCGCGG
GACGTCCTTC
TGCTACGTCC
CTTCGGCCCT
CAATCCAGCG
GACCTTCCTT
CCCGCGGCCT
GCTGCCGGCT
CTGCGGCCTC
TTCCGCGTCT
TCGCCTTCGC
CCTCAGACGA
GTCGGATCTC
CCTTTGGGCC
GCCTCCCCGC
ATCGATACCG
TCGACCAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGGGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ACAAGATCTG
CTTTTTGCTT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGCAT
CTAGAATTAA
TTCCGTGTAT
TCTATAGTGT
CACCTAAATC
GTATGTGTAT
GATACATAAG
GTTATGTATT
AATTGTAGCC
GCGTTCTAAC
GACAATATGT
ACAAGCCTAA
TTGTGTAGCA
TCTGGCTTAC
TGAAGCAGAC
CCTATCATCT
CTCTCGTAAA
CTGCCGTCAG
AGTCGGTTTG
GTTGGACGAA
CCTTCTGAGT
TTCTGGTAAC
GCCGTCCCGC
ACCCGGAAAT
GGTCAGCGAA
CCAATCAGCA
GGGTCATCGC
TAGCCAGATC
CTCTACGCCG
GACGCATCGT
GGCCGGCATC
ACCGGCGCCA
CAGGTGCGGT
TGCTGGCGCC
TATATCGCCG
ACATCACCGA
TGGGGAAGAT
CGGGCTCGCC
ACTTCGGGCT
CATGAGCGCT
TGTTTCGGCG
TGGGTATGGT
GGCAGGCCCC
GTGGCCGGGG
GACTGTTGGG
CGCCATCTCC
TTGCATGCAC
CATTCCTTGC
GGCGGCGGTG
CTCAACGGCC
TCAACCTACT
ACTGGGCTGC
TTCCTAATGC
AGGAGTCGCA
TAAGGGAGAG
CGTCGAATGG
TGCACTCTCA
GTACAATCTA
GCTCTGATGC
CGCATAGTTA
AGCCAGCCCC
GACACCCGCC
AACACCCGCT
GACGCGCCCT
GACGGGCTTG
TCTGCTCCCG
GCATCCGCTT
ACAGACAAGC
TGTGACCGTC
TCCGGGAGCT
GCATGTGTCA
GAGGTTTTCA
CCGTCATCAC
CGAAACGCGC
GAGACGAAAG
GGCCTCGTGA
TACGCCTATT
TTTATAGGTT
AATGTCATGA
TAATAATGGT
TTCTTAGACG
TCAGGTGGCA
CTTTTCGGGG
AAATGTGCGC
GGAACCCCTA
TTTGTTTATT
TTTCTAAATA
CATTCAAATA
TGTATCCGCT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAATATTGA
AAAAGGAAGA
GTATGAGTAT
TCAACATTTC
CGTGTCGCCC
TTATTCCCTT
TTTTGCGGCA
TTTTGCCTTC
CTGTTTTTGC
TCACCCAGAA
ACGCTGGTGA
AAGTAAAAGA
TGCTGAAGAT
CAGTTGGGTG
CACGAGTGGG
TTACATCGAA
CTGGATCTCA
ACAGCGGTAA
GATCCTTGAG
AGTTTTCGCC
CCGAAGAACG
TTTTCCAATG
ATGAGCACTT
TTAAAGTTCT
GCTATGTGGC
GCGGTATTAT
CCCGTATTGA
CGCCGGGCAA
GAGCAACTCG
GTCGCCGCAT
ACACTATTCT
CAGAATGACT
TGGTTGAGTA
CTCACCAGTC
ACAGAAAAGC
ATCTTACGGA
TGGCATGACA
GTAAGAGAAT
TATGCAGTGC
TGCCATAACC
ATGAGTGATA
ACACTGCGGC
CAACTTACTT
CTGACAACGA
TCGGAGGACC
GAAGGAGCTA
ACCGCTTTTT
TGCACAACAT
GGGGGATCAT
GTAACTCGCC
TTGATCGTTG
GGAACCGGAG
CTGAATGAAG
CCATACCAAA
CGACGAGCGT
GACACCACGA
TGCCTGTAGC
AATGGCAACA
ACGTTGCGCA
AACTATTAAC
TGGCGAACTA
CTTACTCTAG
CTTCCCGGCA
ACAATTAATA
GACTGGATGG
AGGCGGATAA
AGTTGCAGGA
CCACTTCTGC
GCTCGGCCCT
TCCGGCTGGC
TGGTTTATTG
CTGATAAATC
TGGAGCCGGT
GAGCGTGGGT
CTCGCGGTAT
CATTGCAGCA
CTGGGGCCAG
ATGGTAAGCC
CTCCCGTATC
GTAGTTATCT
ACACGACGGG
GAGTCAGGCA
ACTATGGATG
AACGAAATAG
ACAGATCGCT
GAGATAGGTG
CCTCACTGAT
TAAGCATTGG
TAACTGTCAG
ACCAAGTTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTTTCGT
TCCACTGAGC
GTCAGACCCC
GTAGAAAAGA
TCAAAGGATC
TTCTTGAGAT
CCTTTTTTTC
TGCGCGTAAT
CTGCTGCTTG
CAAACAAAAA
AACCACCGCT
ACCAGCGGTG
GTTTGTTTGC
CGGATCAAGA
GCTACCAACT
CTTTTTCCGA
AGGTAACTGG
CTTCAGCAGA
GCGCAGATAC
CAAATACTGT
CCTTCTAGTG
TAGCCGTAGT
TAGGCCACCA
CTTCAAGAAC
TCTGTAGCAC
CGCCTACATA
CCTCGCTCTG
CTAATCCTGT
TACCAGTGGC
TGCTGCCAGT
GGCGATAAGT
CGTGTCTTAC
CGGGTTGGAC
TCAAGACGAT
AGTTACCGGA
TAAGGCGCAG
CGGTCGGGCT
GAACGGGGGG
TTCGTGCACA
CAGCCCAGCT
TGGAGCGAAC
GACCTACACC
GAACTGAGAT
ACCTACAGCG
TGAGCTATGA
GAAAGCGCCA
CGCTTCCCGA
AGGGAGAAAG
GCGGACAGGT
ATCCGGTAAG
CGGCAGGGTC
GGAACAGGAG
AGCGCACGAG
GGAGCTTCCA
GGGGGAAACG
CCTGGTATCT
TTATAGTCCT
GTCGGGTTTC
GCCACCTCTG
ACTTGAGCGT
CGATTTTTGT
GATGCTCGTC
AGGGGGGCGG
AGCCTATGGA
AAAACGCCAG
CAACGCGGCC
TTTTTACGGT
TCCTGGCCTT
TTGCTGGCCT
TTTGCTCACA
TGTTCTTTCC
TGCGTTATCC
CCTGATTCTG
TGGATAACCG
TATTACCGCC
TTTGAGTGAG
CTGATACCGC
TCGCCGCAGC
CGAACGACCG
AGCGCAGCGA
GTCAGTGAGC
GAGGAAGCGG
AAGAGCGCCC
AATACGCAAA
CCGCCTCTCC
CCGCGCGTTG
GCCGATTCAT
TAATGCAGCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCGGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAAAG
CTTGGACACA
AGACAGGCTT
GCGAGATATG
TTTGAGAATA
CCACTTTATC
CCGCGTCAGG
GAGAGGCAGT
GCGTAAAAAG
ACGCGGACTC
ATGTGAAATA
CTGGTTTTTA
GTGCGCCAGA
TCTCTATAAT
CTCGCGCAAC
CTATTTTCCC
CTCGAACACT
TTTTAAGCCG
TAGATAAACA
GGCTGGGACA
CTTCACATGA
GCGAAAAATA
CATCGTCACC
TGGGACATGT
TGCAGATCCA
TGCACGTAAA
CTCGCAAGCC
GACTGATGCC
TTCTGAACAA
TGGAAAGGCA
TTATTGCCGT
AAGCCGTGGC
GGTCTGTACC
GGGTGCGTTA
CTGGCGCGTG
AACTGGGTAT
TCGTCATGTC
GATACCGTTT
GTATTTCCAG
CTACGATCAC
GACAACCAGC
GCGAGCTTAA
AGTGCTGAAA
CGCGCAGAAG
GCGATGGCGA
AGGCTTCATC
GTTATTGATG
ACCTGGTGGA
TACCGGTGGT
ACTGCGGTTG
CGATTCGTGA
AATGTATCCA
AAAGCGCACT
TTGTCACCAT
CTTCGCAAAA
CCGGCTGGTC
GTCCGCTGGT
TGATGACTAT
GTTGTTGATA
TCCCGCAAGA
TACCTGGATT
GAACAGCCGT
GGGATATGGG
CGTCGTATTC
GTCCCGCCAA
TCTCCGGTCG
CTAATCTTTT
CAACGCCTGG
CACTGCCGGG
CGTTGTTCTT
TTTAACTTCA
GGCGGGTTAC
AATAGTTTCC
AGTAAGTATT
CTGGAGGCTG
CATCCATGAC
ACAGGCAAAC
CTGAGCGAAA
CCCTGTTCAA
ACCCCGCTTT
AAACATCCTG
AAACCTCGAC
GCTAGTCCGC
CGCTTTAATC
ACGGCGCACA
ACCGCCTGTG
CAGTCGGCCC
TTGATGGTAA
AACCATCCCT
CACTGGTATC
GCATGATTAA
CCGTCTGATG
TGGATCTGGC
GCGGCATTGA
CCCACGCGAA
ATCCTCGACG
TCCAGGCACG
TATTGTGATG
AGCGATGCCG
AACGTACCGA
CGATGATTTA
TACGATACGG
TGATTGGCTA
CCGTGGCGGC
AACTGGATTT
ATGAGTGGGC
CCCGGATCTT
TGTGAAGGAA
CCTTACTTCT
GTGGTGTGAC
ATAATTGGAC
AAACTACCTA
CAGAGATTTA
AAGCTCTAAG
GTAAATATAA
AATTTTTAAG
TGTATAATGT
GTTAAACTAC
TGATTCTAAT
TGTTTGTGTA
TTTTAGATTC
CAACCTATGG
AACTGATGAA
TGGGAGCAGT
GGTGGAATGC
CTTTAATGAG
GAAAACCTGT
TTTGCTCAGA
AGAAATGCCA
TCTAGTGATG
ATGAGGCTAC
TGCTGACTCT
CAACATTCTA
CTCCTCCAAA
AAAGAAGAGA
AAGGTAGAAG
ACCCCAAGGA
CTTTCCTTCA
GAATTGCTAA
GTTTTTTGAG
TCATGCTGTG
TTTAGTAATA
GAACTCTTGC
TTGCTTTGCT
ATTTACACCA
CAAAGGAAAA
AGCTGCACTG
CTATACAAGA
AAATTATGGA
AAAATATTCT
GTAACCTTTA
TAAGTAGGCA
TAACAGTTAT
AATCATAACA
TACTGTTTTT
TCTTACTCCA
CACAGGCATA
GAGTGTCTGC
TATTAATAAC
TATGCTCAAA
AATTGTGTAC
CTTTAGCTTT
TTAATTTGTA
AAGGGGTTAA
TAAGGAATAT
TTGATGTATA
GTGCCTTGAC
TAGAGATCAT
AATCAGCCAT
ACCACATTTG
TAGAGGTTTT
ACTTGCTTTA
AAAAACCTCC
CACACCTCCC
CCTGAACCTG
AAACATAAAA
TGAATGCAAT
TGTTGTTGTT
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGATCAACTG
GATAACTCAA
GCTAACCAAA
ATCATCCCAA
ACTTCCCACC
CCATACCCTA
TTACCACTGC
CAATTACCTA
GTGGTTTCAT
TTACTCTAAA
CCTGTGATTC
CTCTGAATTA
TTTTCATTTT
AAAGAAATTG
TATTTGTTAA
ATATGTACTA
CAAACTTAGT
AGTTGGAAGG
GCTAATTCAC
TCCCAAAGAA
GACAAGATAT
CCTTGATCTG
TGGATCTACC
ACACACAAGG
CTACTTCCCT
GATTAGCAGA
ACTACACACC
AGGGCCAGGG
GTCAGATATC
CACTGACCTT
TGGATGGTGC
TACAAGCTAG
TACCAGTTGA
GCCAGATAAG
GTAGAAGAGG
CCAATAAAGG
AGAGAACACC
AGCTTGTTAC
ACCCTGTGAG
CCTGCATGGG
ATGGATGACC
CGGAGAGAGA
AGTGTTAGAG
TGGAGGTTTG
ACAGCCGCCT
AGCATTTCAT
CACGTGGCCC
GAGAGCTGCA
TCCGGAGTAC
TTCAAGAACT
GCTGATATCG
AGCTTGCTAC
AAGGGACTTT
CCGCTGGGGA
CTTTCCAGGG
AGGCGTGGCC
TGGGCGGGAC
TGGGGAGTGG
CGAGCCCTCA
GATCCTGCAT
ATAAGCAGCT
GCTTTTTGCC
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTG
GCGCCCGAAC
AGGGACTTGA
AAGCGAAAGG
GAAACCAGAG
GAGCTCTCTC
GACGCAGGAC
TCGGCTTGCT
GAAGCGCGCA
CGGCAAGAGG
CGAGGGGCGG
CGACTGGTGA
GTACGCCAAA
AATTTTGACT
AGCGGAGGCT
AGAAGGAGAG
AGATGGGTGC
GAGAGCGTCA
GTATTAAGCG
GGGGAGAATT
AGATCGCGAT
GGGAAAAAAT
TCGGTTAAGG
CCAGGGGGAA
AGAAAAAATA
TAAATTAAAA
CATATAGTAT
GGGCAAGCAG
GGAGCTAGAA
CGATTCGCAG
TTAATCCTGG
CCTGTTAGAA
ACATCAGAAG
GCTGTAGACA
AATACTGGGA
CAGCTACAAC
CATCCCTTCA
GACAGGATCA
GAAGAACTTA
GATCATTATA
TAATACAGTA
GCAACCCTCT
ATTGTGTGCA
TCAAAGGATA
GAGATAAAAG
ACACCAAGGA
AGCTTTAGAC
AAGATAGAGG
AAGAGCAAAA
CAAAAGTAAG
ACCACCGCAC
AGCAAGCGGC
CGGTGATCTT
CAGACCTGGA
CGATATATAT
GAGGGACAAT
TGGAGAAGTG
AATTATATAA
ATATAAAGTA
GTAAAAATTG
AACCATTAGG
AGTAGCACCC
ACCAAGGCAA
AGAGAAGAGT
GGTGCAGAGA
GAAAAAAGAG
CAGTGGGAAT
AGGAGCTTTG
TTCCTTGGGT
TCTTGGGAGC
AGCAGGAAGC
ACTATGGGCG
CAGCGTCAAT
GACGCTGACG
GTACAGGCCA
GACAATTATT
GTCTGGTATA
GTGCAGCAGC
AGAACAATTT
GCTGAGGGCT
ATTGAGGCGC
AACAGCATCT
GTTGCAACTC
ACAGTCTGGG
GCATCAAGCA
GCTCCAGGCA
AGAATCCTGG
CTGTGGAAAG
ATACCTAAAG
GATCAACAGC
TCCTGGGGAT
TTGGGGTTGC
TCTGGAAAAC
TCATTTGCAC
CACTGCTGTG
CCTTGGAATG
CTAGTTGGAG
TAATAAATCT
CTGGAACAGA
TTTGGAATCA
CACGACCTGG
ATGGAGTGGG
ACAGAGAAAT
TAACAATTAC
ACAAGCTTAA
TACACTCCTT
AATTGAAGAA
TCGCAAAACC
AGCAAGAAAA
GAATGAACAA
GAATTATTGG
AATTAGATAA
ATGGGCAAGT
TTGTGGAATT
GGTTTAACAT
AACAAATTGG
CTGTGGTATA
TAAAATTATT
CATAATGATA
GTAGGAGGCT
TGGTAGGTTT
AAGAATAGTT
TTTGCTGTAC
TTTCTATAGT
GAATAGAGTT
AGGCAGGGAT
ATTCACCATT
ATCGTTTCAG
ACCCACCTCC
CAACCCCGAG
GGGACCCGAC
AGGCCCGAAG
GAATAGAAGA
AGAAGGTGGA
GAGAGAGACA
GAGACAGATC
CATTCGATTA
GTGAACGGAT
CTCGACGGTC
G