名称: | pEGAD |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | 35S |
复制子: | oriV |
拷贝数: | 低 |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pROKII |
正向引物: | 35s |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGAGCGTCGC
AAAGGCGCTC
GGTCTTGCCT
TGCTCGTCGG
TGATGTACTT
CACCAGCTCC
GCGAAGTCGC
TCTTCTTGAT
GGAGCGCATG
GGGACGTGCT
TGGCAATCAC
GCGCACCCCC
CGGCCGTTTT
AGCGGCTAAA
AAAGTCATGG
CTCTGCCCTC
GGGCGGACCA
CGCCCATCAT
GACCTTGCCA
AGCTCGTCCT
GCTTCTCTTC
GATCTTCGCC
AGCAGGGCGA
GGATCGTGGC
ATCACCGAAC
CGCGCCGTGC
GCGGGTCGTC
GGTGAGCCAG
AGTTTCAGCA
GGCCGCCCAG
GCGGCCCAGG
TCGCCATTGA
TGCGGGCCAG
CTCGCGGACG
TGCTCATAGT
CCACGACGCC
CGTGATTTTG
TAGCCCTGGC
CGACGGCCAG
CAGGTAGGCC
GACAGGCTCA
TGCCGGCCGC
CGCCGCCTTT
TCCTCAATCG
CTCTTCGTTC
GTCTGGAAGG
CAGTACACCT
TGATAGGTGG
GCTGCCCTTC
CTGGTTGGCT
TGGTTTCATC
AGCCATCCGC
TTGCCCTCAT
CTGTTACGCC
GGCGGTAGCC
GGCCAGCCTC
GCAGAGCAGG
ATTCCCGTTG
AGCACCGCCA
GGTGCGAATA
AGGGACAGTG
AAGAAGGAAC
ACCCGCTCGC
GGGTGGGCCT
ACTTCACCTA
TCCTGCCCGG
CTGACGCCGT
TGGATACACC
AAGGAAAGTC
TACACGAACC
CTTTGGCAAA
ATCCTGTATA
TCGTGCGAAA
AAGGATGGAT
ATACCGAAAA
AATCGCTATA
ATGACCCCGA
AGCAGGGTTA
TGCAGCGGAA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCAGA
AGGCCGCCAG
AGAGGCCGAG
CGCGGCCGTG
AGGCTTGGAC
GCTAGGGCAG
GGCATGAAAA
AGCCCGTAGC
GGGCTGCTAC
GGGCGTCTGA
CGCGGTGGAA
AGGGGGAGGG
GATGTTGTCT
ACATGGCTCT
GCTGTAGTGA
GTGGGTTGCG
CTCCGGCAGC
GGTCCTGATC
AATCGTCACC
CTTTCTCGGT
CCTTCAACGT
TCCTGACAAC
GAGCCTCCTT
TTCGCCAATC
CATCGACAAT
CACCGCGAGT
CCCTGCTCGA
ACGCTGCGTC
CGGACCGGCT
TCGTCGAAGG
CGTCTATCGC
GGCCCGCAAC
AGCGGCGAGA
GCGGAGCCTG
TTCAACGGTG
CCGCCGCGCT
CGCCGGCATC
GCTGTCGCCG
GCCTGCTCCT
CAAGCACGGC
CCCAACAGTG
AAGTAGCTGA
TTGTCATCAG
CGCATTGACG
GCGTCCCCGG
CCGAAAAACC
CGCCTCGCAG
AGGAAGCGAA
GCTGCGCGTC
GGCCGTTTCC
ATCTGCGGTG
CGCCCGGTCG
CGTGCCGGCA
TGGATGCGCG
CGCCATCGCG
GTAGGCGAGC
AGCGCCTGCC
TGAAGCTGCG
GGCATTCCCG
ATCAGAAATG
AGCGCCAGTC
GTCGTCGGCT
CTCGGCACCG
AATGCGTATG
ATTCTCCGCC
AGCATGGCTT
CGGCCAGTGC
GTCGAGCAGC
GCCCGCTTGT
TCCTGAAGTG
CCAGTAAAGC
GCCGGCTGCT
GAACCCCCAA
CCGTTCCGCC
AGTTTGCGTG
TCGTCAGACC
GTCTACGCCG
ACCTCGTTCA
ACAGGTCCAG
GGCGGCACGG
ATCACTGTAT
TCGGCTGCAA
CTTTGTCATG
CTTGACACTT
TATCACTGAT
AAACATAATA
TGTCCACCAA
CTTATCAGTG
ATAAAGAATC
CGCGCGTTCA
ATCGGACCAG
CGGAGGCTGG
TCCGGAGGCC
AGACGTGAAA
CCCAACATAC
CCCTGATCGT
AATTCTGAGC
ACTGTCGCGC
TCGACGCTGT
CGGCATCGGC
CTGATTATGC
CGGTGCTGCC
GGGCCTCCTG
CGCGATCTGG
TTCACTCGAA
CGACGTCACC
GCCCACTATG
GCATTCTGCT
GGCGCTGTAT
GCGTTGGTGC
AATTTGCCTG
CGCACCTGTG
CTGGGCGCGC
TGTCGGATCG
TTTCGGGCGG
CGGCCAATCT
TGCTCGTCTC
GCTGGCCGGC
GCCAGATCTG
GGGAACCCTG
TGGTTGGCAT
GCACATACAA
ATGGACGAAC
GGATAAACCT
TTTCACGCCC
TTTTAAATAT
CCGATTATTC
TAATAAACGC
TCTTTTCTCT
TAGGTTTACC
CGCCAATATA
TCCTGTCAAA
CACTGATAGT
TTAAACTGAA
GGCGGGAAAC
GACAATCTGA
TCATGAGCGG
AGAATTAAGG
GAGTCACGTT
ATGACCCCCG
CCGATGACGC
GGGACAAGCC
GTTTTACGTT
TGGAACTGAC
AGAACCGCAA
CGTTGAAGGA
GCCACTCAGC
CGCGGGTTTC
TGGAGTTTAA
TGAGCTAAGC
ACATACGTCA
GAAACCATTA
TTGCGCGTTC
AAAAGTCGCC
TAAGGTCACT
ATCAGCTAGC
GGGCAATTGA
GACTTTTCAA
CAAAGGGTAA
TATCCGGAAA
CCTCCTCGGA
TTCCATTGCC
CAGCTATCTG
TCACTTTATT
GTGAAGATAG
TGGAAAAGGA
AGGTGGCTCC
TACAAATGCC
ATCATTGCGA
TAAAGGAAAG
GCCATCGTTG
AAGATGCCTC
TGCCGACAGT
GGTCCCAAAG
ATGGACCCCC
ACCCACGAGG
AGCATCGTGG
AAAAAGAAGA
CGTTCCAACC
ACGTCTTCAA
AGCAAGTGGA
TTGATGTGAT
ATCTCCACTG
ACGTAAGGGA
TGACGCACAA
TCCCACTATC
CTTCGCAAGA
CCCTTCCTCT
ATATAAAGAA
GTTCATTTCA
TTTGGAGAGA
ACACGGGGGA
CGAGCTCATG
GTGAGCAAGG
GCGAGGAGCT
GTTCACCGGG
GTGGTGCCCA
TCCTGGTCGA
GCTGGACGGC
GACGTAAACG
GCCACAAGTT
CAGCGTGTCC
GGCGAGGGCG
AGGGCGATGC
CACCTACGGC
AAGCTGACCC
TGAAGTTCAT
CTGCACCACC
GGCAAGCTGC
CCGTGCCCTG
GCCCACCCTC
GTGACCACCC
TGACCTACGG
CGTGCAGTGC
TTCAGCCGCT
ACCCCGACCA
CATGAAGCAG
CACGACTTCT
TCAAGTCCGC
CATGCCCGAA
GGCTACGTCC
AGGAGCGCAC
CATCTTCTTC
AAGGACGACG
GCAACTACAA
GACCCGCGCC
GAGGTGAAGT
TCGAGGGCGA
CACCCTGGTG
AACCGCATCG
AGCTGAAGGG
CATCGACTTC
AAGGAGGACG
GCAACATCCT
GGGGCACAAG
CTGGAGTACA
ACTACAACAG
CCACAACGTC
TATATCATGG
CCGACAAGCA
GAAGAACGGC
ATCAAGGTGA
ACTTCAAGAT
CCGCCACAAC
ATCGAGGACG
GCAGCGTGCA
GCTCGCCGAC
CACTACCAGC
AGAACACCCC
CATCGGCGAC
GGCCCCGTGC
TGCTGCCCGA
CAACCACTAC
CTGAGCACCC
AGTCCGCCCT
GAGCAAAGAC
CCCAACGAGA
AGCGCGATCA
CATGGTCCTG
CTGGAGTTCG
TGACCGCCGC
CGGGATCACT
CTCGGCATGG
ACGAGCTGTA
CAAGAAGAAG
GGCGGAAAGA
TCGCCGTGGG
AGGTGGCTCA
TCTGGCGGAG
GTCAGATCTC
GTACGCGTCC
CGGGGCGGTA
CCCGGGATCC
TCTAGAGGGC
CCGGCGCGCC
ACTAGTGTCG
ACCTGCAGCT
GATCTCGAGG
AGCTAGCTCG
AATTTCCCCG
ATCGTTCAAA
CATTTGGCAA
TAAAGTTTCT
TAAGATTGAA
TCCTGTTGCC
GGTCTTGCGA
TGATTATCAT
ATAATTTCTG
TTGAATTACG
TTAAGCATGT
AATAATTAAC
ATGTAATGCA
TGACGTTATT
TATGAGATGG
GTTTTTATGA
TTAGAGTCCC
GCAATTATAC
ATTTAATACG
CGATAGAAAA
CAAAATATAG
CGCGCAAACT
AGGATAAATT
ATCGCGCGCG
GTGTCATCTA
TGTTACTAGA
TCGGGAGTAC
TATTCCAGTA
TGGACGATTC
AAGGCTTGCT
TCACAAACCA
AGGCAAGTAA
TAGAGATTGG
AGTCTCTAAA
AAGGTAGTTC
CCACTGAATC
AAAGGCCATG
GAGTCAAAGA
TTCAAATAGA
GGACCTAACA
GAACTCGCCG
TAAAGACTGG
CGAACAGTTC
ATACAGAGTC
TCTTACGACT
CAATGACAAG
AAGAAAATCT
TCGTCAACAT
GGTGGAGCAC
GACACACTTG
TCTACTCCAA
AAATATCAAA
GATACAGTCT
CAGAAGACCA
AAGGGCAATT
GAGACTTTTC
AACAAAGGGT
AATATCCGGA
AACCTCCTCG
GATTCCATTG
CCCAGCTATC
TGTCACTTTA
TTGTGAAGAT
AGTGGAAAAG
GAAGGTGGCT
CCTACAAATG
CCATCATTGC
GATAAAGGAA
AGGCCATCGT
TGAAGATGCC
TCTGCCGACA
GTGGTCCCAA
AGATGGACCC
CCACCCACGA
GGAGCATCGT
GGAAAAAGAA
GACGTTCCAA
CCACGTCTTC
AAAGCAAGTG
GATTGATGTG
ATATCTCCAC
TGACGTAAGG
GATGACGCAC
AATCCCACTA
TCCTTCGCAA
GACCCTTCCT
CTATATAAGG
AAGTTCATTT
CATTTGGAGA
GAACACGGGG
GACTCTAGAC
CATGAGCCCA
GAACGACGCC
CGGCCGACAT
CCGCCGTGCC
ACCGAGGCGG
ACATGCCGGC
GGTCTGCACC
ATCGTCAACC
ACTACATCGA
GACAAGCACG
GTCAACTTCC
GTACCGAGCC
GCAGGAACCG
CAGGAGTGGA
CGGACGACCT
CGTCCGTCTG
CGGGAGCGCT
ATCCCTGGCT
CGTCGCCGAG
GTGGACGGCG
AGGTCGCCGG
CATCGCCTAC
GCGGGCCCCT
GGAAGGCACG
CAACGCCTAC
GACTGGACGG
CCGAGTCGAC
CGTGTACGTC
TCCCCCCGCC
ACCAGCGGAC
GGGACTGGGC
TCCACGCTCT
ACACCCACCT
GCTGAAGTCC
CTGGAGGCAC
AGGGCTTCAA
GAGCGTGGTC
GCTGTCATCG
GGCTGCCCAA
CGACCCGAGC
GTGCGCATGC
ACGAGGCGCT
CGGATATGCC
CCCCGCGGCA
TGCTGCGGGC
GGCCGGCTTC
AAGCACGGGA
ACTGGCATGA
CGTGGGTTTC
TGGCAGCTGG
ACTTCAGCCT
GCCGGTACCG
CCCCGTCCGG
TCCTGCCCGT
CACCGAGATC
TGAGGTACCG
AGCTCGAATT
TCCCCGATCG
TTCAAACATT
TGGCAATAAA
GTTTCTTAAG
ATTGAATCCT
GTTGCCGGTC
TTGCGATGAT
TATCATATAA
TTTCTGTTGA
ATTACGTTAA
GCATGTAATA
ATTAACATGT
AATGCATGAC
GTTATTTATG
AGATGGGTTT
TTATGATTAG
AGTCCCGCAA
TTATACATTT
AATACGCGAT
AGAAAACAAA
ATATAGCGCG
CAAACTAGGA
TAAATTATCG
CGCGCGGTGT
CATCTATGTT
ACTAGATCGG
GAATTCACTG
GCCGTCGTTT
TACAACGTCG
TGACTGGGAA
AACCCTGGCG
TTACCCAACT
TAATCGCCTT
GCAGCACATC
CCCCTTTCGC
CAGCTGGCGT
AATAGCGAAG
AGGCCCGCAC
CGATCGCCCT
TCCCAACAGT
TGCGCAGCCT
GAATGGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TTGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGGCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGAACC
ACCATCAAAC
AGGATTTTCG
CCTGCTGGGG
CAAACCAGCG
TGGACCGCTT
GCTGCAACTC
TCTCAGGGCC
AGGCGGTGAA
GGGCAATCAG
CTGTTGCCCG
TCTCACTGGT
GAAAAGAAAA
ACCACCCCAG
TACATTAAAA
ACGTCCGCAA
TGTGTTATTA
AGTTGTCTAA
GCGTCAATTT
GTTTACACCA
CAATATATCC
TGCCACCAGC
CAGCCAACAG
CTCCCCGACC
GGCAGCTCGG
CACAAAATCA
CCACTCGATA
CAGGCAGCCC
ATCAGTCCGG
GACGGCGTCA
GCGGGAGAGC
CGTTGTAAGG
CGGCAGACTT
TGCTCATGTT
ACCGATGCTA
TTCGGAAGAA
CGGCAACTAA
GCTGCCGGGT
TTGAAACACG
GATGATCTCG
CGGAGGGTAG
CATGTTGATT
GTAACGATGA
CAGAGCGTTG
CTGCCTGTGA
TCAAATATCA
TCTCCCTCGC
AGAGATCCGA
ATTATCAGCC
TTCTTATTCA
TTTCTCGCTT
AACCGTGACA
GGCTGTCGAT
CTTGAGAACT
ATGCCGACAT
AATAGGAAAT
CGCTGGATAA
AGCCGCTGAG
GAAGCTGAGT
GGCGCTATTT
CTTTAGAAGT
GAACGTTGAC
GATATCAACT
CCCCTATCCA
TTGCTCACCG
AATGGTACAG
GTCGGGGACC
CGAAGTTCCG
ACTGTCGGCC
TGATGCATCC
CCGGCTGATC
GACCCCAGAT
CTGGGGCTGA
GAAAGCCCAG
TAAGGAAACA
ACTGTAGGTT
CGAGTCGCGA
GATCCCCCGG
AACCAAAGGA
AGTAGGTTAA
ACCCGCTCCG
ATCAGGCCGA
GCCACGCCAG
GCCGAGAACA
TTGGTTCCTG
TAGGCATCGG
GATTGGCGGA
TCAAACACTA
AAGCTACTGG
AACGAGCAGA
AGTCCTCCGG
CCGCCAGTTG
CCAGGCGGTA
AAGGTGAGCA
GAGGCACGGG
AGGTTGCCAC
TTGCGGGTCA
GCACGGTTCC
GAACGCCATG
GAAACCGCCC
CCGCCAGGCC
CGCTGCGACG
CCGACAGGAT
CTAGCGCTGC
GTTTGGTGTC
AACACCAACA
GCGCCACGCC
CGCAGTTCCG
CAAATAGCCC
CCAGGACCGC
CATCAATCGT
ATCGGGCTAC
CTAGCAGAGC
GGCAGAGATG
AACACGACCA
TCAGCGGCTG
CACAGCGCCT
ACCGTCGCCG
CGACCCCGCC
CGGCAGGCGG
TAGACCGAAA
TAAACAACAA
GCTCCAGAAT
AGCGAAATAT
TAAGTGCGCC
GAGGATGAAG
ATGCGCATCC
ACCAGATTCC
CGTTGGAATC
TGTCGGACGA
TCATCACGAG
CAATAAACCC
GCCGGCAACG
CCCGCAGCAG
CATACCGGCG
ACCCCTCGGC
CTCGCTGTTC
GGGCTCCACG
AAAACGCCGG
ACAGATGCGC
CTTGTGAGCG
TCCTTGGGGC
CGTCCTCCTG
TTTGAAGACC
GACAGCCCAA
TGATCTCGCC
GTCGATGTAG
GCGCCGAATG
CCACGGCATC
TCGCAACCGT
TCAGCGAACG
CCTCCATGGG
CTTTTTCTCC
TCGTGCTCGT
AAACGGACCC
GAACATCTCT
GGAGCTTTCT
TCAGGGCCGA
CAATCGGATC
TCGCGGAAAT
CCTGCACGTC
GGCCGCTCCA
AGCCGTCGAA
TCTGAGCCTT
AATCACAATT
GTCAATTTTA
ATCCTCTGTT
TATCGGCAGT
TCGTAGAGCG
CGCCGTGCGT
CCCGAGCGAT
ACTGAGCGAA
GCAAGTGCGT
CGAGCAGTGC
CCGCTTGTTC
CTGAAATGCC
AGTAAAGCGC
TGGCTGCTGA
ACCCCCAGCC
GGAACTGACC
CCACAAGGCC
CTAGCGTTTG
CAATGCACCA
GGTCATCATT
GACCCAGGCG
TGTTCCACCA
GGCCGCTGCC
TCGCAACTCT
TCGCAGGCTT
CGCCGACCTG
CTCGCGCCAC
TTCTTCACGC
GGGTGGAATC
CGATCCGCAC
ATGAGGCGGA
AGGTTTCCAG
CTTGAGCGGG
TACGGCTCCC
GGTGCGAGCT
GAAATAGTCG
AACATCCGTC
GGGCCGTCGG
CGACAGCTTG
CGGTACTTCT
CCCATATGAA
TTTCGTGTAG
TGGTCGCCAG
CAAACAGCAC
GACGATTTCC
TCGTCGATCA
GGACCTGGCA
ACGGGACGTT
TTCTTGCCAC
GGTCCAGGAC
GCGGAAGCGG
TGCAGCAGCG
ACACCGATTC
CAGGTGCCCA
ACGCGGTCGG
ACGTGAAGCC
CATCGCCGTC
GCCTGTAGGC
GCGACAGGCA
TTCCTCGGCC
TTCGTGTAAT
ACCGGCCATT
GATCGACCAG
CCCAGGTCCT
GGCAAAGCTC
GTAGAACGTG
AAGGTGATCG
GCTCGCCGAT
AGGGGTGCGC
TTCGCGTACT
CCAACACCTG
CTGCCACACC
AGTTCGTCAT
CGTCGGCCCG
CAGCTCGACG
CCGGTGTAGG
TGATCTTCAC
GTCCTTGTTG
ACGTGGAAAA
TGACCTTGTT
TTGCAGCGCC
TCGCGCGGGA
TTTTCTTGTT
GCGCGTGGTG
AACAGGGCAG
AGCGGGCCGT
GTCGTTTGGC
ATCGCTCGCA
TCGTGTCCGG
CCACGGCGCA
ATATCGAACA
AGGAAAGCTG
CATTTCCTTG
ATCTGCTGCT
TCGTGTGTTT
CAGCAACGCG
GCCTGCTTGG
CCTCGCTGAC
CTGTTTTGCC
AGGTCCTCGC
CGGCGGTTTT
TCGCTTCTTG
GTCGTCATAG
TTCCTCGCGT
GTCGATGGTC
ATCGACTTCG
CCAAACCTGC
CGCCTCCTGT
TCGAGACGAC
GCGAACGCTC
CACGGCGGCC
GATGGCGCGG
GCAGGGCAGG
GGGAGCCAGT
TGCACGCTGT
CGCGCTCGAT
CTTGGCCGTA
GCTTGCTGGA
CCATCGAGCC
GACGGACTGG
AAGGTTTCGC
GGGGCGCACG
CATGACGGTG
CGGCTTGCGA
TGGTTTCGGC
ATCCTCGGCG
GAAAACCCCG
CGTCGATCAG
TTCTTGCCTG
TATGCCTTCC
GGTCAAACGT
CCGATTCATT
CACCCTCCTT
GCGGGATTGC
CCCGACTCAC
GCCGGGGCAA
TGTGCCCTTA
TTCCTGATTT
GACCCGCCTG
GTGCCTTGGT
GTCCAGATAA
TCCACCTTAT
CGGCAATGAA
GTCGGTCCCG
TAGACCGTCT
GGCCGTCCTT
CTCGTACTTG
GTATTCCGAA
TCTTGCCCTG
CACGAATACC
AGCGACCCCT
TGCCCAAATA
CTTGCCGTGG
GCCTCGGCCT
GAGAGCCAAA
ACACTTGATG
CGGAAGAAGT
CGGTGCGCTC
CTGCTTGTCG
CCGGCATCGT
TGCGCCACAT
CTAGGTACTA
AAACAATTCA
TCCAGTAAAA
TATAATATTT
TATTTTCTCC
CAATCAGGCT
TGATCCCCAG
TAAGTCAAAA
AATAGCTCGA
CATACTGTTC
TTCCCCGATA
TCCTCCCTGA
TCGACCGGAC
GCAGAAGGCA
ATGTCATACC
ACTTGTCCGC
CCTGCCGCTT
CTCCCAAGAT
CAATAAAGCC
ACTTACTTTG
CCATCTTTCA
CAAAGATGTT
GCTGTCTCCC
AGGTCGCCGT
GGGAAAAGAC
AAGTTCCTCT
TCGGGCTTTT
CCGTCTTTAA
AAAATCATAC
AGCTCGCGCG
GATCTTTAAA
TGGAGTGTCT
TCTTCCCAGT
TTTCGCAATC
CACATCGGCC
AGATCGTTAT
TCAGTAAGTA
ATCCAATTCG
GCTAAGCGGC
TGTCTAAGCT
ATTCGTATAG
GGACAATCCG
ATATGTCGAT
GGAGTGAAAG
AGCCTGATGC
ACTCCGCATA
CAGCTCGATA
ATCTTTTCAG
GGCTTTGTTC
ATCTTCATAC
TCTTCCGAGC
AAAGGACGCC
ATCGGCCTCA
CTCATGAGCA
GATTGCTCCA
GCCATCATGC
CGTTCAAAGT
GCAGGACCTT
TGGAACAGGC
AGCTTTCCTT
CCAGCCATAG
CATCATGTCC
TTTTCCCGTT
CCACATCATA
GGTGGTCCCT
TTATACCGGC
TGTCCGTCAT
TTTTAAATAT
AGGTTTTCAT
TTTCTCCCAC
CAGCTTATAT
ACCTTAGCAG
GAGACATTCC
TTCCGTATCT
TTTACGCAGC
GGTATTTTTC
GATCAGTTTT
TTCAATTCCG
GTGATATTCT
CATTTTAGCC
ATTTATTATT
TCCTTCCTCT
TTTCTACAGT
ATTTAAAGAT
ACCCCAAGAA
GCTAATTATA
ACAAGACGAA
CTCCAATTCA
CTGTTCCTTG
CATTCTAAAA
CCTTAAATAC
CAGAAAACAG
CTTTTTCAAA
GTTGTTTTCA
AAGTTGGCGT
ATAACATAGT
ATCGACGGAG
CCGATTTTGA
AACCACAATT
ATGGGTGATG
CTGCCAACTT
ACTGATTTAG
TGTATGATGG
TGTTTTTGAG
GTGCTCCAGT
GGCTTCTGTG
TCTATCAGCT
GTCCCTCCTG
TTCAGCTACT
GACGGGGTGG
TGCGTAACGG
CAAAAGCACC
GCCGGACATC
AGCGCTATCT
CTGCTCTCAC
TGCCGTAAAA
CATGGCAACT
GCAGTTCACT
TACACCGCTT
CTCAACCCGG
TACGCACCAG
AAAATCATTG
ATATGGCCAT
GAATGGCGTT
GGATGCCGGG
CAACAGCCCG
CATTATGGGC
GTTGGCCTCA
ACACGATTTT
ACGTCACTTA
AAAAACTCAG
GCCGCAGTCG
GTAACCTCGC
GCATACAGCC
GGGCAGTGAC
GTCATCGTCT
GCGCGGAAAT
GGACGAACAG
TGGGGCTATG
TCGGGGCTAA
ATCGCGCCAG
CGCTGGCTGT
TTTACGCGTA
TGACAGTCTC
CGGAAGACGG
TTGTTGCGCA
CGTATTCGGT
GAACGCACTA
TGGCGACGCT
GGGGCGTCTT
ATGAGCCTGC
TGTCACCCTT
TGACGTGGTG
ATATGGATGA
CGGATGGCTG
GCCGCTGTAT
GAATCCCGCC
TGAAGGGAAA
GCTGCACGTA
ATCAGCAAGC
GATATACGCA
GCGAATTGAG
CGGCATAACC
TGAATCTGAG
GCAGCACCTG
GCACGGCTGG
GACGGAAGTC
GCTGTCGTTC
TCAAAATCGG
TGGAGCTGCA
TGACAAAGTC
ATCGGGCATT
ATCTGAACAT
AAAACACTAT
CAATAAGTTG
GAGTCATTAC
CCAATTATGA
TAGAATTTAC
AAGCTATAAG
GTTATTGTCC
TGGGTTTCAA
GCATTAGTCC
ATGCAAGTTT
TTATGCTTTG
CCCATTCTAT
AGATATATTG
ATAAGCGCGC
TGCCTATGCC
TTGCCCCCTG
AAATCCTTAC
ATACGGCGAT
ATCTTCTATA
TAAAAGATAT
ATTATCTTAT
CAGTATTGTC
AATATATTCA
AGGCAATCTG
CCTCCTCATC
CTCTTCATCC
TCTTCGTCTT
GGTAGCTTTT
TAAATATGGC
GCTTCATAGA
GTAATTCTGT
AAAGGTCCAA
TTCTCGTTTC
ATACCTCGGT
ATAATCTTAC
CTATCACCTC
AAATGGTTCG
CTGGGTTTAT
CGCACCCCCG
AACACGAGCA
CGGCACCCGC
GACCACTATG
CCAAGAATGC
CCAAGGTAAA
AATTGCCGGC
CCCGCCATGA
AGTCCGTGAA
TGCCCCGACG
GCCGAAGTGA
AGGGCAGGCC
GCCACCAGGC
CGCCGCCCTC
ACTGCCCGGC
ACCTGGTCGC
TGAATGTCGA
TGCCAGCACC
TGCGGCACGT
CAATGCTTCC
GGGCGTCGCG
CTCGGGCTGA
TCGCCCATCC
CGTTACTGCC
CCGATCCCGG
CAATGGCAAG
GACTGCCAGC
GCTGCCATTT
TTGGGGTGAG
GCCGTTCGCG
GCCGAGGGGC
GCAGCCCCTG
GGGGGATGGG
AGGCCCGCGT
TAGCGGGCCG
GGAGGGTTCG
AGAAGGGGGG
GCACCCCCCT
TCGGCGTGCG
CGGTCACGCG
CACAGGGCGC
AGCCCTGGTT
AAAAACAAGG
TTTATAAATA
TTGGTTTAAA
AGCAGGTTAA
AAGACAGGTT
AGCGGTGGCC
GAAAAACGGG
CGGAAACCCT
TGCAAATGCT
GGATTTTCTG
CCTGTGGACA
GCCCCTCAAA
TGTCAATAGG
TGCGCCCCTC
ATCTGTCAGC
ACTCTGCCCC
TCAAGTGTCA
AGGATCGCGC
CCCTATCTGT
CAGTAGTCGC
GCCCCTCAAG
TGTCAATACC
GCAGGGCACT
TATCCCCAGG
CTTGTCCACA
TCATCTGTGG
GAAACTCGCG
TAAAATCAGG
CGTTTTCGCC
GATTTGCGAG
GCTGGCCAGC
TCCACGTCGC
CGGCCGAAAT
CGAGCCTGCC
CCTCATCTGT
CAACGCCGCG
CCGGGTGAGT
CGGCCCCTCA
AGTGTCAACG
TCCGCCCCTC
ATCTGTCAGT
GAGGGCCAAG
TTTTCCGCGA
GGTATCCACA
ACGCCGGCGG
CCGCGGTGTC
TCGCACACGG
CTTCGACGGC
GTTTCTGGCG
CGTTTGCAGG
GCCATAGACG
GCCGCCAGCC
CAGCGGCGAG
GGCAACCAGC
CCGG