名称: | pCMV-HA-RRBP1(human) |
---|---|
别称: | NM_001365613.2;ES/130/ES130/hES/p180/RRp |
基因长: | 4233bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-HA-N |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAGTTCGAGC
TTGCATGCCT
GCAGGTCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAATGA
CGTATGTTCC
CATAGTAACG
CCAATAGGGA
CTTTCCATTG
ACGTCAATGG
GTGGAGTATT
TACGGTAAAC
TGCCCACTTG
GCAGTACATC
AAGTGTATCA
TATGCCAAGT
ACGCCCCCTA
TTGACGTCAA
TGACGGTAAA
TGGCCCGCCT
GGCATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTCT
ATATAAGCAG
AGCTCGTTTA
GTGAACCGTC
AGATCGCCTG
GAGACGCCAT
CCACGCTGTT
TTGACCTCCA
TAGAAGACAC
CGGGACCGAT
CCAGCCTCCG
GACTCTAGAG
GATCCGGTAC
TAGAGGAACT
GAAAAACCAG
AAAGTTAACT
GGTAAGTTTA
GTCTTTTTGT
CTTTTATTTC
AGGTCCCGGA
TCCGGTGGTG
GTGCAAATCA
AAGAACTGCT
CCTCAGTGGA
TGTTGCCTTT
ACTTCTAGGC
CTGTACGGAA
GTGTTACTTC
TGCTCTAAAA
GCTGCGGAAT
TGTACCCGCG
GGCCCACCAT
GTACCCATAC
GATGTTCCAG
ATTACGCTCT
TATGGCCATG
GAGGCCCGAA
TTATGGATAT
TTACGACACT
CAAACCTTGG
GGGTTGTGGT
CTTTGGAGGA
TTCATGGTTG
TTTCTGCCAT
TGGCATCTTC
CTGGTGTCGA
CTTTCTCCAT
GAAGGAAACG
TCATATGAAG
AAGCCCTAGC
CAACCAGCGC
AAGGAGATGG
CGAAAACTCA
CCACCAGAAA
GTCGAGAAGA
AAAAGAAGGA
GAAAACAGTG
GAGAAGAAAG
GAAAGACCAA
GAAAAAGGAA
GAGAAACCTA
ATGGGAAGAT
ACCTGATCAT
GATCCAGCCC
CCAATGTGAC
TGTCCTCCTT
CGAGAACCAG
TGCGGGCTCC
TGCTGTGGCT
GTGGCTCCAA
CCCCAGTGCA
GCCCCCCATT
ATCGTTGCTC
CTGTCGCCAC
AGTTCCAGCC
ATGCCCCAGG
AGAAGCTGGC
CTCCTCCCCC
AAGGACAAAA
AGAAGAAGGA
GAAAAAAGTG
GCAAAAGTGG
AACCAGCTGT
CAGCTCTGTA
GTGAATTCCA
TCCAGGTTCT
CACTTCGAAG
GCTGCCATCT
TGGAAACTGC
TCCCAAGGAG
GTGCCGATGG
TGGTGGTGCC
CCCAGTGGGT
GCCAAGGGCA
ACACACCAGC
CACTGGCACT
ACTCAGGGCA
AAAAGGCGGA
GGGGACTCAG
AATCAAAGCA
AAAAGGCTGA
AGGAGCCCCA
AACCAGGGCA
GAAAGGCAGA
GGGAACCCCA
AACCAGGGCA
AAAAGACAGA
GGGAACCCCA
AACCAAGGGA
AAAAGGCAGA
GGGAACCCCA
AACCAAGGCA
AAAAGGCAGA
AGGAACCCCA
AACCAAGGCA
AAAAGGCGGA
GGGGGCCCAG
AACCAGGGTA
AAAAGGTAGA
TACAACCCCA
AACCAGGGGA
AAAAGGTGGA
GGGGGCCCCA
ACCCAGGGCA
GAAAGGCCGA
GGGGGCTCAG
AACCAGGCCA
AAAAGGTAGA
AGGGGCCCAG
AACCAGGGCA
AAAAGGCAGA
GGGGGCCCAG
AATCAGGGCA
AAAAGGGAGA
GGGGGCCCAG
AACCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AATCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AATCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AATCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AATCAGGGCA
AGAAGGCTGA
GGGGGCTCAG
AACCAGGGCA
AAAAGGCCGA
GGGGGCTCAG
AACCAGGGCA
AAAAAGTAGA
AGGGGCCCAG
AACCAGGGCA
AGAAGGCTGA
GGGTGCCCAG
AACCAGGGCA
AAAAGGCCGA
GGGGGCCCAG
AATCAGGGCA
AAAAGGCCGA
GGGGGCCCAG
AACCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AACCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AACCAGGGCA
AGAAGGCCGA
GGGGGCCCAG
AACCAGGGCA
AGAAGGCCGA
GGGTGCTCAG
AACCAGGGCA
AAAAAGTAGA
AGGGGCCCAG
AACCAGGGCA
AGAAGGCTGA
GGGGGCCCAG
AACCAGGGCA
AGAAGGCCGA
GGGGGCTCAG
AACCAGGGCA
AAAAGGCCGA
GGGAGCCCAG
AACCAGGGCC
AAAAAGGAGA
GGGAGCCCAG
AATCAGGGTA
AAAAGACAGA
AGGGGCTCAG
GGCAAAAAGG
CAGAAAGGAG
TCCCAACCAA
GGCAAAAAAG
GAGAGGGAGC
TCCCATCCAG
GGCAAAAAGG
CAGATTCGGT
TGCTAATCAG
GGCACAAAGG
TAGAGGGTAT
TACAAACCAG
GGGAAAAAAG
CAGAAGGGTC
CCCCAGTGAA
GGCAAAAAGG
CAGAAGGGTC
CCCCAACCAA
GGCAAAAAGG
CAGACGCAGC
TGCCAATCAG
GGTAAAAAGA
CAGAGTCAGC
TTCTGTCCAG
GGCAGAAATA
CAGATGTGGC
CCAGAGCCCA
GAGGCACCAA
AGCAAGAGGC
TCCTGCCAAG
AAGAAGTCTG
GTTCAAAGAA
AAAAGGTGAG
CCTGGGCCCC
CAGATGCCGA
CGGCCCTCTC
TACCTCCCCT
ACAAGACGCT
GGTCTCCACG
GTTGGGAGCA
TGGTGTTCAA
CGAGGGCGAG
GCCCAGCGGC
TCATCGAGAT
CCTGTCTGAG
AAGGCTGGCA
TCATTCAGGA
CACCTGGCAC
AAGGCCACTC
AGAAGGGTGA
CCCTGTGGCG
ATTCTGAAAC
GCCAGCTGGA
AGAGAAGGAA
AAACTGCTGG
CCACAGAACA
GGAAGATGCG
GCTGTCGCCA
AGAGCAAACT
GAGGGAGCTC
AACAAGGAGA
TGGCAGCAGA
AAAGGCCAAA
GCAGCAGCCG
GGGAGGCCAA
AGTGAAAAAG
CAGCTGGTGG
CCCGGGAGCA
GGAGATCACG
GCTGTGCAGG
CACGCATGCA
GGCCAGCTAC
CGGGAGCACG
TGAAGGAGGT
GCAGCAGCTG
CAGGGCAAGA
TCCGGACTCT
TCAGGAGCAG
CTGGAGAATG
GCCCCAACAC
GCAGCTGGCC
CGCCTGCAGC
AGGAGAACTC
CATCCTGCGG
GATGCCTTGA
ACCAGGCCAC
GAGCCAGGTG
GAGAGCAAGC
AGAACGCAGA
GCTGGCCAAG
CTTCGGCAGG
AGCTCAGCAA
GGTCAGCAAA
GAGCTGGTGG
AGAAGTCAGA
GGCTGTGCGG
CAAGATGAGC
AGCAGCGGAA
AGCTCTGGAA
GCCAAGGCAG
CTGCCTTCGA
GAAGCAGGTC
CTGCAGCTGC
AGGCGTCCCA
CAGGGAGAGT
GAGGAGGCCC
TGCAGAAGCG
CCTGGACGAG
GTCAGCCGGG
AGCTGTGCCA
CACGCAGAGC
AGCCACGCCA
GCCTCCGGGC
GGATGCCGAG
AAGGCCCAGG
AGCAACAGCA
GCAGATGGCC
GAGCTGCACA
GCAAGTTACA
GTCCTCCGAG
GCGGAGGTGC
GCAGCAAATG
CGAGGAGCTG
AGTGGCCTCC
ACGGGCAGCT
CCAGGAGGCC
AGGGCGGAGA
ACTCCCAGCT
CACAGAGAGA
ATCCGTTCCA
TTGAGGCCCT
GCTGGAGGCG
GGCCAGGCGC
GGGATGCCCA
GGACGTCCAG
GCCAGCCAGG
CGGAGGCTGA
CCAGCAGCAG
ACTCGCCTCA
AGGAGCTGGA
GTCCCAGGTG
TCGGGTCTGG
AGAAGGAGGC
CATCGAGCTC
AGGGAGGCCG
TCGAGCAGCA
GAAAGTGAAG
AACAATGACC
TCCGGGAGAA
GAACTGGAAG
GCCATGGAGG
CACTGGCCAC
GGCCGAGCAG
GCCTGCAAGG
AGAAGCTGCT
CTCCCTGACC
CAGGCCAAGG
AGGAATCGGA
GAAGCAGCTC
TGTCTGATTG
AGGCGCAGAC
CATGGAGGCC
CTGCTGGCTC
TGCTCCCAGA
ACTCTCTGTC
TTGGCACAAC
AGAATTACAC
CGAGTGGCTG
CAGGATCTCA
AAGAGAAAGG
CCCCACGCTG
CTGAAGCACC
CGCCAGCTCC
CGCGGAGCCC
TCCTCGGACC
TGGCCTCCAA
GTTGAGGGAG
GCCGAGGAGA
CGCAGAGCAC
ACTGCAGGCC
GAGTGTGACC
AGTACCGCAG
CATCCTGGCG
GAGACGGAGG
GCATGCTCAG
AGACCTGCAG
AAGAGCGTGG
AGGAGGAGGA
GCAGGTGTGG
AGGGCCAAGG
TGGGCGCCGC
AGAGGAGGAG
CTCCAGAAGT
CCCGGGTCAC
AGTGAAGCAT
CTCGAAGAGA
TTGTAGAGAA
GCTAAAAGGA
GAACTTGAAA
GTTCGGACCA
GGTGAGGGAG
CACACGTCGC
ATTTGGAGGC
AGAGCTGGAA
AAGCACATGG
CGGCCGCCAG
CGCCGAGTGC
CAGAACTACG
CCAAGGAGGT
GGCAGGGCTG
AGGCAACTTC
TCCTAGAATC
TCAATCTCAG
CTCGATGCCG
CCAAGAGCGA
AGCCCAGAAA
CAGAGCGATG
AGCTTGCCCT
GGTCAGGCAG
CAGTTGAGTG
AAATGAAGAG
CCACGTAGAG
GATGGTGACA
TAGCTGGGGC
CCCAGCTTCC
TCCCCAGAGG
CGCCCCCAGC
CGAGCAGGAC
CCCGTTCAGC
TGAAGACGCA
GCTGGAGTGG
ACAGAAGCCA
TCCTGGAGGA
TGAGCAGACA
CAGCGGCAGA
AGCTCACGGC
CGAGTTTGAG
GAGGCTCAGA
CCTCGGCATG
TCGGTTACAA
GAAGAATTGG
AGAAGCTCCG
CACAGCCGGC
CCCCTAGAGT
CTTCAGAAAC
AGAGGAGGCC
TCACAGCTGA
AGGAGAGACT
AGAAAAAGAG
AAGAAGTTAA
CAAGTGACCT
GGGGCGCGCC
GCCACGAGAC
TGCAGGAGCT
TCTGAAGACG
ACCCAGGAGC
AGCTGGCAAG
GGAGAAGGAC
ACGGTGAAGA
AGCTGCAGGA
ACAGCTGGAA
AAGGCAGAGG
ACGGCAGCAG
CTCAAAGGAG
GGCACCTCTG
TCTGACTCGA
GGTACCGCGG
CCGCGGGGAT
CCAGACATGA
TAAGATACAT
TGATGAGTTT
GGACAAACCA
CAACTAGAAT
GCAGTGAAAA
AAATGCTTTA
TTTGTGAAAT
TTGTGATGCT
ATTGCTTTAT
TTGTAACCAT
TATAAGCTGC
AATAAACAAG
TTAACAACAA
CAATTGCATT
CATTTTATGT
TTCAGGTTCA
GGGGGAGGTG
TGGGAGGTTT
TTTCGGATCC
TCTAGAGTCG
ATCTGCAGGC
ATGCTAGCTT
GGCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGAACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCATA
TGCGGTGTGA
AATACCGCAC
AGATGCGTAA
GGAGAAAATA
CCGCATCAGG
CGCCATTCGC
CATTCAGGCT
GCGCAACTGT
TGGGAAGGGC
GATCGGTGCG
GGCCTCTTCG
CTATTACGCC
AGCTGGCGAA
AGGGGGATGT
GCTGCAAGGC
GATTAAGTTG
GGTAACGCCA
GGGTTTTCCC
AGTCACGACG
TTGTAAAACG
ACGGCCAGT