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pCMV-HA-RRBP1(human)

产品货号:P64688

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCMV-HA-RRBP1(human)
别称: NM_001365613.2;ES/130/ES130/hES/p180/RRp
基因长: 4233bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体: pCMV-HA-N
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GAGTTCGAGC

TTGCATGCCT

GCAGGTCGTT

ACATAACTTA

CGGTAAATGG

CCCGCCTGGC

61

TGACCGCCCA

ACGACCCCCG

CCCATTGACG

TCAATAATGA

CGTATGTTCC

CATAGTAACG

121

CCAATAGGGA

CTTTCCATTG

ACGTCAATGG

GTGGAGTATT

TACGGTAAAC

TGCCCACTTG

181

GCAGTACATC

AAGTGTATCA

TATGCCAAGT

ACGCCCCCTA

TTGACGTCAA

TGACGGTAAA

241

TGGCCCGCCT

GGCATTATGC

CCAGTACATG

ACCTTATGGG

ACTTTCCTAC

TTGGCAGTAC

301

ATCTACGTAT

TAGTCATCGC

TATTACCATG

GTGATGCGGT

TTTGGCAGTA

CATCAATGGG

361

CGTGGATAGC

GGTTTGACTC

ACGGGGATTT

CCAAGTCTCC

ACCCCATTGA

CGTCAATGGG

421

AGTTTGTTTT

GGCACCAAAA

TCAACGGGAC

TTTCCAAAAT

GTCGTAACAA

CTCCGCCCCA

481

TTGACGCAAA

TGGGCGGTAG

GCGTGTACGG

TGGGAGGTCT

ATATAAGCAG

AGCTCGTTTA

541

GTGAACCGTC

AGATCGCCTG

GAGACGCCAT

CCACGCTGTT

TTGACCTCCA

TAGAAGACAC

601

CGGGACCGAT

CCAGCCTCCG

GACTCTAGAG

GATCCGGTAC

TAGAGGAACT

GAAAAACCAG

661

AAAGTTAACT

GGTAAGTTTA

GTCTTTTTGT

CTTTTATTTC

AGGTCCCGGA

TCCGGTGGTG

721

GTGCAAATCA

AAGAACTGCT

CCTCAGTGGA

TGTTGCCTTT

ACTTCTAGGC

CTGTACGGAA

781

GTGTTACTTC

TGCTCTAAAA

GCTGCGGAAT

TGTACCCGCG

GGCCCACCAT

GTACCCATAC

841

GATGTTCCAG

ATTACGCTCT

TATGGCCATG

GAGGCCCGAA

TTATGGATAT

TTACGACACT

901

CAAACCTTGG

GGGTTGTGGT

CTTTGGAGGA

TTCATGGTTG

TTTCTGCCAT

TGGCATCTTC

961

CTGGTGTCGA

CTTTCTCCAT

GAAGGAAACG

TCATATGAAG

AAGCCCTAGC

CAACCAGCGC

1021

AAGGAGATGG

CGAAAACTCA

CCACCAGAAA

GTCGAGAAGA

AAAAGAAGGA

GAAAACAGTG

1081

GAGAAGAAAG

GAAAGACCAA

GAAAAAGGAA

GAGAAACCTA

ATGGGAAGAT

ACCTGATCAT

1141

GATCCAGCCC

CCAATGTGAC

TGTCCTCCTT

CGAGAACCAG

TGCGGGCTCC

TGCTGTGGCT

1201

GTGGCTCCAA

CCCCAGTGCA

GCCCCCCATT

ATCGTTGCTC

CTGTCGCCAC

AGTTCCAGCC

1261

ATGCCCCAGG

AGAAGCTGGC

CTCCTCCCCC

AAGGACAAAA

AGAAGAAGGA

GAAAAAAGTG

1321

GCAAAAGTGG

AACCAGCTGT

CAGCTCTGTA

GTGAATTCCA

TCCAGGTTCT

CACTTCGAAG

1381

GCTGCCATCT

TGGAAACTGC

TCCCAAGGAG

GTGCCGATGG

TGGTGGTGCC

CCCAGTGGGT

1441

GCCAAGGGCA

ACACACCAGC

CACTGGCACT

ACTCAGGGCA

AAAAGGCGGA

GGGGACTCAG

1501

AATCAAAGCA

AAAAGGCTGA

AGGAGCCCCA

AACCAGGGCA

GAAAGGCAGA

GGGAACCCCA

1561

AACCAGGGCA

AAAAGACAGA

GGGAACCCCA

AACCAAGGGA

AAAAGGCAGA

GGGAACCCCA

1621

AACCAAGGCA

AAAAGGCAGA

AGGAACCCCA

AACCAAGGCA

AAAAGGCGGA

GGGGGCCCAG

1681

AACCAGGGTA

AAAAGGTAGA

TACAACCCCA

AACCAGGGGA

AAAAGGTGGA

GGGGGCCCCA

1741

ACCCAGGGCA

GAAAGGCCGA

GGGGGCTCAG

AACCAGGCCA

AAAAGGTAGA

AGGGGCCCAG

1801

AACCAGGGCA

AAAAGGCAGA

GGGGGCCCAG

AATCAGGGCA

AAAAGGGAGA

GGGGGCCCAG

1861

AACCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

AATCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

1921

AATCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

AATCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

1981

AATCAGGGCA

AGAAGGCTGA

GGGGGCTCAG

AACCAGGGCA

AAAAGGCCGA

GGGGGCTCAG

2041

AACCAGGGCA

AAAAAGTAGA

AGGGGCCCAG

AACCAGGGCA

AGAAGGCTGA

GGGTGCCCAG

2101

AACCAGGGCA

AAAAGGCCGA

GGGGGCCCAG

AATCAGGGCA

AAAAGGCCGA

GGGGGCCCAG

2161

AACCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

AACCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

2221

AACCAGGGCA

AGAAGGCCGA

GGGGGCCCAG

AACCAGGGCA

AGAAGGCCGA

GGGTGCTCAG

2281

AACCAGGGCA

AAAAAGTAGA

AGGGGCCCAG

AACCAGGGCA

AGAAGGCTGA

GGGGGCCCAG

2341

AACCAGGGCA

AGAAGGCCGA

GGGGGCTCAG

AACCAGGGCA

AAAAGGCCGA

GGGAGCCCAG

2401

AACCAGGGCC

AAAAAGGAGA

GGGAGCCCAG

AATCAGGGTA

AAAAGACAGA

AGGGGCTCAG

2461

GGCAAAAAGG

CAGAAAGGAG

TCCCAACCAA

GGCAAAAAAG

GAGAGGGAGC

TCCCATCCAG

2521

GGCAAAAAGG

CAGATTCGGT

TGCTAATCAG

GGCACAAAGG

TAGAGGGTAT

TACAAACCAG

2581

GGGAAAAAAG

CAGAAGGGTC

CCCCAGTGAA

GGCAAAAAGG

CAGAAGGGTC

CCCCAACCAA

2641

GGCAAAAAGG

CAGACGCAGC

TGCCAATCAG

GGTAAAAAGA

CAGAGTCAGC

TTCTGTCCAG

2701

GGCAGAAATA

CAGATGTGGC

CCAGAGCCCA

GAGGCACCAA

AGCAAGAGGC

TCCTGCCAAG

2761

AAGAAGTCTG

GTTCAAAGAA

AAAAGGTGAG

CCTGGGCCCC

CAGATGCCGA

CGGCCCTCTC

2821

TACCTCCCCT

ACAAGACGCT

GGTCTCCACG

GTTGGGAGCA

TGGTGTTCAA

CGAGGGCGAG

2881

GCCCAGCGGC

TCATCGAGAT

CCTGTCTGAG

AAGGCTGGCA

TCATTCAGGA

CACCTGGCAC

2941

AAGGCCACTC

AGAAGGGTGA

CCCTGTGGCG

ATTCTGAAAC

GCCAGCTGGA

AGAGAAGGAA

3001

AAACTGCTGG

CCACAGAACA

GGAAGATGCG

GCTGTCGCCA

AGAGCAAACT

GAGGGAGCTC

3061

AACAAGGAGA

TGGCAGCAGA

AAAGGCCAAA

GCAGCAGCCG

GGGAGGCCAA

AGTGAAAAAG

3121

CAGCTGGTGG

CCCGGGAGCA

GGAGATCACG

GCTGTGCAGG

CACGCATGCA

GGCCAGCTAC

3181

CGGGAGCACG

TGAAGGAGGT

GCAGCAGCTG

CAGGGCAAGA

TCCGGACTCT

TCAGGAGCAG

3241

CTGGAGAATG

GCCCCAACAC

GCAGCTGGCC

CGCCTGCAGC

AGGAGAACTC

CATCCTGCGG

3301

GATGCCTTGA

ACCAGGCCAC

GAGCCAGGTG

GAGAGCAAGC

AGAACGCAGA

GCTGGCCAAG

3361

CTTCGGCAGG

AGCTCAGCAA

GGTCAGCAAA

GAGCTGGTGG

AGAAGTCAGA

GGCTGTGCGG

3421

CAAGATGAGC

AGCAGCGGAA

AGCTCTGGAA

GCCAAGGCAG

CTGCCTTCGA

GAAGCAGGTC

3481

CTGCAGCTGC

AGGCGTCCCA

CAGGGAGAGT

GAGGAGGCCC

TGCAGAAGCG

CCTGGACGAG

3541

GTCAGCCGGG

AGCTGTGCCA

CACGCAGAGC

AGCCACGCCA

GCCTCCGGGC

GGATGCCGAG

3601

AAGGCCCAGG

AGCAACAGCA

GCAGATGGCC

GAGCTGCACA

GCAAGTTACA

GTCCTCCGAG

3661

GCGGAGGTGC

GCAGCAAATG

CGAGGAGCTG

AGTGGCCTCC

ACGGGCAGCT

CCAGGAGGCC

3721

AGGGCGGAGA

ACTCCCAGCT

CACAGAGAGA

ATCCGTTCCA

TTGAGGCCCT

GCTGGAGGCG

3781

GGCCAGGCGC

GGGATGCCCA

GGACGTCCAG

GCCAGCCAGG

CGGAGGCTGA

CCAGCAGCAG

3841

ACTCGCCTCA

AGGAGCTGGA

GTCCCAGGTG

TCGGGTCTGG

AGAAGGAGGC

CATCGAGCTC

3901

AGGGAGGCCG

TCGAGCAGCA

GAAAGTGAAG

AACAATGACC

TCCGGGAGAA

GAACTGGAAG

3961

GCCATGGAGG

CACTGGCCAC

GGCCGAGCAG

GCCTGCAAGG

AGAAGCTGCT

CTCCCTGACC

4021

CAGGCCAAGG

AGGAATCGGA

GAAGCAGCTC

TGTCTGATTG

AGGCGCAGAC

CATGGAGGCC

4081

CTGCTGGCTC

TGCTCCCAGA

ACTCTCTGTC

TTGGCACAAC

AGAATTACAC

CGAGTGGCTG

4141

CAGGATCTCA

AAGAGAAAGG

CCCCACGCTG

CTGAAGCACC

CGCCAGCTCC

CGCGGAGCCC

4201

TCCTCGGACC

TGGCCTCCAA

GTTGAGGGAG

GCCGAGGAGA

CGCAGAGCAC

ACTGCAGGCC

4261

GAGTGTGACC

AGTACCGCAG

CATCCTGGCG

GAGACGGAGG

GCATGCTCAG

AGACCTGCAG

4321

AAGAGCGTGG

AGGAGGAGGA

GCAGGTGTGG

AGGGCCAAGG

TGGGCGCCGC

AGAGGAGGAG

4381

CTCCAGAAGT

CCCGGGTCAC

AGTGAAGCAT

CTCGAAGAGA

TTGTAGAGAA

GCTAAAAGGA

4441

GAACTTGAAA

GTTCGGACCA

GGTGAGGGAG

CACACGTCGC

ATTTGGAGGC

AGAGCTGGAA

4501

AAGCACATGG

CGGCCGCCAG

CGCCGAGTGC

CAGAACTACG

CCAAGGAGGT

GGCAGGGCTG

4561

AGGCAACTTC

TCCTAGAATC

TCAATCTCAG

CTCGATGCCG

CCAAGAGCGA

AGCCCAGAAA

4621

CAGAGCGATG

AGCTTGCCCT

GGTCAGGCAG

CAGTTGAGTG

AAATGAAGAG

CCACGTAGAG

4681

GATGGTGACA

TAGCTGGGGC

CCCAGCTTCC

TCCCCAGAGG

CGCCCCCAGC

CGAGCAGGAC

4741

CCCGTTCAGC

TGAAGACGCA

GCTGGAGTGG

ACAGAAGCCA

TCCTGGAGGA

TGAGCAGACA

4801

CAGCGGCAGA

AGCTCACGGC

CGAGTTTGAG

GAGGCTCAGA

CCTCGGCATG

TCGGTTACAA

4861

GAAGAATTGG

AGAAGCTCCG

CACAGCCGGC

CCCCTAGAGT

CTTCAGAAAC

AGAGGAGGCC

4921

TCACAGCTGA

AGGAGAGACT

AGAAAAAGAG

AAGAAGTTAA

CAAGTGACCT

GGGGCGCGCC

4981

GCCACGAGAC

TGCAGGAGCT

TCTGAAGACG

ACCCAGGAGC

AGCTGGCAAG

GGAGAAGGAC

5041

ACGGTGAAGA

AGCTGCAGGA

ACAGCTGGAA

AAGGCAGAGG

ACGGCAGCAG

CTCAAAGGAG

5101

GGCACCTCTG

TCTGACTCGA

GGTACCGCGG

CCGCGGGGAT

CCAGACATGA

TAAGATACAT

5161

TGATGAGTTT

GGACAAACCA

CAACTAGAAT

GCAGTGAAAA

AAATGCTTTA

TTTGTGAAAT

5221

TTGTGATGCT

ATTGCTTTAT

TTGTAACCAT

TATAAGCTGC

AATAAACAAG

TTAACAACAA

5281

CAATTGCATT

CATTTTATGT

TTCAGGTTCA

GGGGGAGGTG

TGGGAGGTTT

TTTCGGATCC

5341

TCTAGAGTCG

ATCTGCAGGC

ATGCTAGCTT

GGCGTAATCA

TGGTCATAGC

TGTTTCCTGT

5401

GTGAAATTGT

TATCCGCTCA

CAATTCCACA

CAACATACGA

GCCGGAAGCA

TAAAGTGTAA

5461

AGCCTGGGGT

GCCTAATGAG

TGAGCTAACT

CACATTAATT

GCGTTGCGCT

CACTGCCCGC

5521

TTTCCAGTCG

GGAAACCTGT

CGTGCCAGCT

GCATTAATGA

ATCGGCCAAC

GCGCGGGGAG

5581

AGGCGGTTTG

CGTATTGGGC

GCTCTTCCGC

TTCCTCGCTC

ACTGACTCGC

TGCGCTCGGT

5641

CGTTCGGCTG

CGGCGAGCGG

TATCAGCTCA

CTCAAAGGCG

GTAATACGGT

TATCCACAGA

5701

ATCAGGGGAT

AACGCAGGAA

AGAACATGTG

AGCAAAAGGC

CAGCAAAAGG

CCAGGAACCG

5761

TAAAAAGGCC

GCGTTGCTGG

CGTTTTTCCA

TAGGCTCCGC

CCCCCTGACG

AGCATCACAA

5821

AAATCGACGC

TCAAGTCAGA

GGTGGCGAAA

CCCGACAGGA

CTATAAAGAT

ACCAGGCGTT

5881

TCCCCCTGGA

AGCTCCCTCG

TGCGCTCTCC

TGTTCCGACC

CTGCCGCTTA

CCGGATACCT

5941

GTCCGCCTTT

CTCCCTTCGG

GAAGCGTGGC

GCTTTCTCAT

AGCTCACGCT

GTAGGTATCT

6001

CAGTTCGGTG

TAGGTCGTTC

GCTCCAAGCT

GGGCTGTGTG

CACGAACCCC

CCGTTCAGCC

6061

CGACCGCTGC

GCCTTATCCG

GTAACTATCG

TCTTGAGTCC

AACCCGGTAA

GACACGACTT

6121

ATCGCCACTG

GCAGCAGCCA

CTGGTAACAG

GATTAGCAGA

GCGAGGTATG

TAGGCGGTGC

6181

TACAGAGTTC

TTGAAGTGGT

GGCCTAACTA

CGGCTACACT

AGAAGAACAG

TATTTGGTAT

6241

CTGCGCTCTG

CTGAAGCCAG

TTACCTTCGG

AAAAAGAGTT

GGTAGCTCTT

GATCCGGCAA

6301

ACAAACCACC

GCTGGTAGCG

GTGGTTTTTT

TGTTTGCAAG

CAGCAGATTA

CGCGCAGAAA

6361

AAAAGGATCT

CAAGAAGATC

CTTTGATCTT

TTCTACGGGG

TCTGACGCTC

AGTGGAACGA

6421

AAACTCACGT

TAAGGGATTT

TGGTCATGAG

ATTATCAAAA

AGGATCTTCA

CCTAGATCCT

6481

TTTAAATTAA

AAATGAAGTT

TTAAATCAAT

CTAAAGTATA

TATGAGTAAA

CTTGGTCTGA

6541

CAGTTACCAA

TGCTTAATCA

GTGAGGCACC

TATCTCAGCG

ATCTGTCTAT

TTCGTTCATC

6601

CATAGTTGCC

TGACTCCCCG

TCGTGTAGAT

AACTACGATA

CGGGAGGGCT

TACCATCTGG

6661

CCCCAGTGCT

GCAATGATAC

CGCGAGACCC

ACGCTCACCG

GCTCCAGATT

TATCAGCAAT

6721

AAACCAGCCA

GCCGGAAGGG

CCGAGCGCAG

AAGTGGTCCT

GCAACTTTAT

CCGCCTCCAT

6781

CCAGTCTATT

AATTGTTGCC

GGGAAGCTAG

AGTAAGTAGT

TCGCCAGTTA

ATAGTTTGCG

6841

CAACGTTGTT

GCCATTGCTA

CAGGCATCGT

GGTGTCACGC

TCGTCGTTTG

GTATGGCTTC

6901

ATTCAGCTCC

GGTTCCCAAC

GATCAAGGCG

AGTTACATGA

TCCCCCATGT

TGTGCAAAAA

6961

AGCGGTTAGC

TCCTTCGGTC

CTCCGATCGT

TGTCAGAAGT

AAGTTGGCCG

CAGTGTTATC

7021

ACTCATGGTT

ATGGCAGCAC

TGCATAATTC

TCTTACTGTC

ATGCCATCCG

TAAGATGCTT

7081

TTCTGTGACT

GGTGAGTACT

CAACCAAGTC

ATTCTGAGAA

TAGTGTATGC

GGCGACCGAG

7141

TTGCTCTTGC

CCGGCGTCAA

TACGGGATAA

TACCGCGCCA

CATAGCAGAA

CTTTAAAAGT

7201

GCTCATCATT

GGAAAACGTT

CTTCGGGGCG

AAAACTCTCA

AGGATCTTAC

CGCTGTTGAG

7261

ATCCAGTTCG

ATGTAACCCA

CTCGTGCACC

CAACTGATCT

TCAGCATCTT

TTACTTTCAC

7321

CAGCGTTTCT

GGGTGAGCAA

AAACAGGAAG

GCAAAATGCC

GCAAAAAAGG

GAATAAGGGC

7381

GACACGGAAA

TGTTGAATAC

TCATACTCTT

CCTTTTTCAA

TATTATTGAA

GCATTTATCA

7441

GGGTTATTGT

CTCATGAGCG

GATACATATT

TGAATGTATT

TAGAAAAATA

AACAAATAGG

7501

GGTTCCGCGC

ACATTTCCCC

GAAAAGTGCC

ACCTGACGTC

TAAGAAACCA

TTATTATCAT

7561

GACATTAACC

TATAAAAATA

GGCGTATCAC

GAGGCCCTTT

CGTCTCGCGC

GTTTCGGTGA

7621

TGACGGTGAA

AACCTCTGAC

ACATGCAGCT

CCCGGAGACG

GTCACAGCTT

GTCTGTAAGC

7681

GGATGCCGGG

AGCAGACAAG

CCCGTCAGGG

CGCGTCAGCG

GGTGTTGGCG

GGTGTCGGGG

7741

CTGGCTTAAC

TATGCGGCAT

CAGAGCAGAT

TGTACTGAGA

GTGCACCATA

TGCGGTGTGA

7801

AATACCGCAC

AGATGCGTAA

GGAGAAAATA

CCGCATCAGG

CGCCATTCGC

CATTCAGGCT

7861

GCGCAACTGT

TGGGAAGGGC

GATCGGTGCG

GGCCTCTTCG

CTATTACGCC

AGCTGGCGAA

7921

AGGGGGATGT

GCTGCAAGGC

GATTAAGTTG

GGTAACGCCA

GGGTTTTCCC

AGTCACGACG

7981

TTGTAAAACG

ACGGCCAGT

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