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pT7-OPA1(human)-6×His

产品货号:P64650

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pT7-OPA1(human)-6×His
别称: NM_130837.3;BERHS; largeG; MGM1; MTDPS14; NPG; NTG
基因长: 3048bp
质粒宿主: 大肠杆菌
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Kan
筛选标记:
荧光标记:
启动子: T7
复制子: pBR322
拷贝数:
感受态: DH5a
温度: 37度
背景载体: pET-28a
正向引物: T7
反向引物:
诱导方式: IPTG

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TGGCGAATGG

GACGCGCCCT

GTAGCGGCGC

ATTAAGCGCG

GCGGGTGTGG

TGGTTACGCG

61

CAGCGTGACC

GCTACACTTG

CCAGCGCCCT

AGCGCCCGCT

CCTTTCGCTT

TCTTCCCTTC

121

CTTTCTCGCC

ACGTTCGCCG

GCTTTCCCCG

TCAAGCTCTA

AATCGGGGGC

TCCCTTTAGG

181

GTTCCGATTT

AGTGCTTTAC

GGCACCTCGA

CCCCAAAAAA

CTTGATTAGG

GTGATGGTTC

241

ACGTAGTGGG

CCATCGCCCT

GATAGACGGT

TTTTCGCCCT

TTGACGTTGG

AGTCCACGTT

301

CTTTAATAGT

GGACTCTTGT

TCCAAACTGG

AACAACACTC

AACCCTATCT

CGGTCTATTC

361

TTTTGATTTA

TAAGGGATTT

TGCCGATTTC

GGCCTATTGG

TTAAAAAATG

AGCTGATTTA

421

ACAAAAATTT

AACGCGAATT

TTAACAAAAT

ATTAACGTTT

ACAATTTCAG

GTGGCACTTT

481

TCGGGGAAAT

GTGCGCGGAA

CCCCTATTTG

TTTATTTTTC

TAAATACATT

CAAATATGTA

541

TCCGCTCATG

AATTAATTCT

TAGAAAAACT

CATCGAGCAT

CAAATGAAAC

TGCAATTTAT

601

TCATATCAGG

ATTATCAATA

CCATATTTTT

GAAAAAGCCG

TTTCTGTAAT

GAAGGAGAAA

661

ACTCACCGAG

GCAGTTCCAT

AGGATGGCAA

GATCCTGGTA

TCGGTCTGCG

ATTCCGACTC

721

GTCCAACATC

AATACAACCT

ATTAATTTCC

CCTCGTCAAA

AATAAGGTTA

TCAAGTGAGA

781

AATCACCATG

AGTGACGACT

GAATCCGGTG

AGAATGGCAA

AAGTTTATGC

ATTTCTTTCC

841

AGACTTGTTC

AACAGGCCAG

CCATTACGCT

CGTCATCAAA

ATCACTCGCA

TCAACCAAAC

901

CGTTATTCAT

TCGTGATTGC

GCCTGAGCGA

GACGAAATAC

GCGATCGCTG

TTAAAAGGAC

961

AATTACAAAC

AGGAATCGAA

TGCAACCGGC

GCAGGAACAC

TGCCAGCGCA

TCAACAATAT

1021

TTTCACCTGA

ATCAGGATAT

TCTTCTAATA

CCTGGAATGC

TGTTTTCCCG

GGGATCGCAG

1081

TGGTGAGTAA

CCATGCATCA

TCAGGAGTAC

GGATAAAATG

CTTGATGGTC

GGAAGAGGCA

1141

TAAATTCCGT

CAGCCAGTTT

AGTCTGACCA

TCTCATCTGT

AACATCATTG

GCAACGCTAC

1201

CTTTGCCATG

TTTCAGAAAC

AACTCTGGCG

CATCGGGCTT

CCCATACAAT

CGATAGATTG

1261

TCGCACCTGA

TTGCCCGACA

TTATCGCGAG

CCCATTTATA

CCCATATAAA

TCAGCATCCA

1321

TGTTGGAATT

TAATCGCGGC

CTAGAGCAAG

ACGTTTCCCG

TTGAATATGG

CTCATAACAC

1381

CCCTTGTATT

ACTGTTTATG

TAAGCAGACA

GTTTTATTGT

TCATGACCAA

AATCCCTTAA

1441

CGTGAGTTTT

CGTTCCACTG

AGCGTCAGAC

CCCGTAGAAA

AGATCAAAGG

ATCTTCTTGA

1501

GATCCTTTTT

TTCTGCGCGT

AATCTGCTGC

TTGCAAACAA

AAAAACCACC

GCTACCAGCG

1561

GTGGTTTGTT

TGCCGGATCA

AGAGCTACCA

ACTCTTTTTC

CGAAGGTAAC

TGGCTTCAGC

1621

AGAGCGCAGA

TACCAAATAC

TGTCCTTCTA

GTGTAGCCGT

AGTTAGGCCA

CCACTTCAAG

1681

AACTCTGTAG

CACCGCCTAC

ATACCTCGCT

CTGCTAATCC

TGTTACCAGT

GGCTGCTGCC

1741

AGTGGCGATA

AGTCGTGTCT

TACCGGGTTG

GACTCAAGAC

GATAGTTACC

GGATAAGGCG

1801

CAGCGGTCGG

GCTGAACGGG

GGGTTCGTGC

ACACAGCCCA

GCTTGGAGCG

AACGACCTAC

1861

ACCGAACTGA

GATACCTACA

GCGTGAGCTA

TGAGAAAGCG

CCACGCTTCC

CGAAGGGAGA

1921

AAGGCGGACA

GGTATCCGGT

AAGCGGCAGG

GTCGGAACAG

GAGAGCGCAC

GAGGGAGCTT

1981

CCAGGGGGAA

ACGCCTGGTA

TCTTTATAGT

CCTGTCGGGT

TTCGCCACCT

CTGACTTGAG

2041

CGTCGATTTT

TGTGATGCTC

GTCAGGGGGG

CGGAGCCTAT

GGAAAAACGC

CAGCAACGCG

2101

GCCTTTTTAC

GGTTCCTGGC

CTTTTGCTGG

CCTTTTGCTC

ACATGTTCTT

TCCTGCGTTA

2161

TCCCCTGATT

CTGTGGATAA

CCGTATTACC

GCCTTTGAGT

GAGCTGATAC

CGCTCGCCGC

2221

AGCCGAACGA

CCGAGCGCAG

CGAGTCAGTG

AGCGAGGAAG

CGGAAGAGCG

CCTGATGCGG

2281

TATTTTCTCC

TTACGCATCT

GTGCGGTATT

TCACACCGCA

TATATGGTGC

ACTCTCAGTA

2341

CAATCTGCTC

TGATGCCGCA

TAGTTAAGCC

AGTATACACT

CCGCTATCGC

TACGTGACTG

2401

GGTCATGGCT

GCGCCCCGAC

ACCCGCCAAC

ACCCGCTGAC

GCGCCCTGAC

GGGCTTGTCT

2461

GCTCCCGGCA

TCCGCTTACA

GACAAGCTGT

GACCGTCTCC

GGGAGCTGCA

TGTGTCAGAG

2521

GTTTTCACCG

TCATCACCGA

AACGCGCGAG

GCAGCTGCGG

TAAAGCTCAT

CAGCGTGGTC

2581

GTGAAGCGAT

TCACAGATGT

CTGCCTGTTC

ATCCGCGTCC

AGCTCGTTGA

GTTTCTCCAG

2641

AAGCGTTAAT

GTCTGGCTTC

TGATAAAGCG

GGCCATGTTA

AGGGCGGTTT

TTTCCTGTTT

2701

GGTCACTGAT

GCCTCCGTGT

AAGGGGGATT

TCTGTTCATG

GGGGTAATGA

TACCGATGAA

2761

ACGAGAGAGG

ATGCTCACGA

TACGGGTTAC

TGATGATGAA

CATGCCCGGT

TACTGGAACG

2821

TTGTGAGGGT

AAACAACTGG

CGGTATGGAT

GCGGCGGGAC

CAGAGAAAAA

TCACTCAGGG

2881

TCAATGCCAG

CGCTTCGTTA

ATACAGATGT

AGGTGTTCCA

CAGGGTAGCC

AGCAGCATCC

2941

TGCGATGCAG

ATCCGGAACA

TAATGGTGCA

GGGCGCTGAC

TTCCGCGTTT

CCAGACTTTA

3001

CGAAACACGG

AAACCGAAGA

CCATTCATGT

TGTTGCTCAG

GTCGCAGACG

TTTTGCAGCA

3061

GCAGTCGCTT

CACGTTCGCT

CGCGTATCGG

TGATTCATTC

TGCTAACCAG

TAAGGCAACC

3121

CCGCCAGCCT

AGCCGGGTCC

TCAACGACAG

GAGCACGATC

ATGCGCACCC

GTGGGGCCGC

3181

CATGCCGGCG

ATAATGGCCT

GCTTCTCGCC

GAAACGTTTG

GTGGCGGGAC

CAGTGACGAA

3241

GGCTTGAGCG

AGGGCGTGCA

AGATTCCGAA

TACCGCAAGC

GACAGGCCGA

TCATCGTCGC

3301

GCTCCAGCGA

AAGCGGTCCT

CGCCGAAAAT

GACCCAGAGC

GCTGCCGGCA

CCTGTCCTAC

3361

GAGTTGCATG

ATAAAGAAGA

CAGTCATAAG

TGCGGCGACG

ATAGTCATGC

CCCGCGCCCA

3421

CCGGAAGGAG

CTGACTGGGT

TGAAGGCTCT

CAAGGGCATC

GGTCGAGATC

CCGGTGCCTA

3481

ATGAGTGAGC

TAACTTACAT

TAATTGCGTT

GCGCTCACTG

CCCGCTTTCC

AGTCGGGAAA

3541

CCTGTCGTGC

CAGCTGCATT

AATGAATCGG

CCAACGCGCG

GGGAGAGGCG

GTTTGCGTAT

3601

TGGGCGCCAG

GGTGGTTTTT

CTTTTCACCA

GTGAGACGGG

CAACAGCTGA

TTGCCCTTCA

3661

CCGCCTGGCC

CTGAGAGAGT

TGCAGCAAGC

GGTCCACGCT

GGTTTGCCCC

AGCAGGCGAA

3721

AATCCTGTTT

GATGGTGGTT

AACGGCGGGA

TATAACATGA

GCTGTCTTCG

GTATCGTCGT

3781

ATCCCACTAC

CGAGATATCC

GCACCAACGC

GCAGCCCGGA

CTCGGTAATG

GCGCGCATTG

3841

CGCCCAGCGC

CATCTGATCG

TTGGCAACCA

GCATCGCAGT

GGGAACGATG

CCCTCATTCA

3901

GCATTTGCAT

GGTTTGTTGA

AAACCGGACA

TGGCACTCCA

GTCGCCTTCC

CGTTCCGCTA

3961

TCGGCTGAAT

TTGATTGCGA

GTGAGATATT

TATGCCAGCC

AGCCAGACGC

AGACGCGCCG

4021

AGACAGAACT

TAATGGGCCC

GCTAACAGCG

CGATTTGCTG

GTGACCCAAT

GCGACCAGAT

4081

GCTCCACGCC

CAGTCGCGTA

CCGTCTTCAT

GGGAGAAAAT

AATACTGTTG

ATGGGTGTCT

4141

GGTCAGAGAC

ATCAAGAAAT

AACGCCGGAA

CATTAGTGCA

GGCAGCTTCC

ACAGCAATGG

4201

CATCCTGGTC

ATCCAGCGGA

TAGTTAATGA

TCAGCCCACT

GACGCGTTGC

GCGAGAAGAT

4261

TGTGCACCGC

CGCTTTACAG

GCTTCGACGC

CGCTTCGTTC

TACCATCGAC

ACCACCACGC

4321

TGGCACCCAG

TTGATCGGCG

CGAGATTTAA

TCGCCGCGAC

AATTTGCGAC

GGCGCGTGCA

4381

GGGCCAGACT

GGAGGTGGCA

ACGCCAATCA

GCAACGACTG

TTTGCCCGCC

AGTTGTTGTG

4441

CCACGCGGTT

GGGAATGTAA

TTCAGCTCCG

CCATCGCCGC

TTCCACTTTT

TCCCGCGTTT

4501

TCGCAGAAAC

GTGGCTGGCC

TGGTTCACCA

CGCGGGAAAC

GGTCTGATAA

GAGACACCGG

4561

CATACTCTGC

GACATCGTAT

AACGTTACTG

GTTTCACATT

CACCACCCTG

AATTGACTCT

4621

CTTCCGGGCG

CTATCATGCC

ATACCGCGAA

AGGTTTTGCG

CCATTCGATG

GTGTCCGGGA

4681

TCTCGACGCT

CTCCCTTATG

CGACTCCTGC

ATTAGGAAGC

AGCCCAGTAG

TAGGTTGAGG

4741

CCGTTGAGCA

CCGCCGCCGC

AAGGAATGGT

GCATGCAAGG

AGATGGCGCC

CAACAGTCCC

4801

CCGGCCACGG

GGCCTGCCAC

CATACCCACG

CCGAAACAAG

CGCTCATGAG

CCCGAAGTGG

4861

CGAGCCCGAT

CTTCCCCATC

GGTGATGTCG

GCGATATAGG

CGCCAGCAAC

CGCACCTGTG

4921

GCGCCGGTGA

TGCCGGCCAC

GATGCGTCCG

GCGTAGAGGA

TCGAGATCTC

GATCCCGCGA

4981

AATTAATACG

ACTCACTATA

GGGGAATTGT

GAGCGGATAA

CAATTCCCCT

CTAGAAATAA

5041

TTTTGTTTAA

CTTTAAGAAG

GAGATATACC

ATGGGCAGCA

GCCATCATCA

TCATCATCAC

5101

AGCAGCGGCC

TGGTGCCGCG

CGGCAGCCAT

ATGGCTAGCA

TGACTGGTGG

ACAGCAAATG

5161

GGTCGCGGAT

CCATGTGGCG

ACTACGTCGG

GCCGCTGTGG

CCTGTGAGGT

CTGCCAGTCT

5221

TTAGTGAAAC

ACAGCTCTGG

AATAAAAGGA

AGTTTACCAC

TACAAAAACT

ACATCTGGTT

5281

TCACGAAGCA

TTTATCATTC

ACATCATCCT

ACCTTAAAGC

TTCAACGACC

CCAATTAAGG

5341

ACATCCTTTC

AGCAGTTCTC

TTCTCTGACA

AACCTTCCTT

TACGTAAACT

GAAATTCTCT

5401

CCAATTAAAT

ATGGCTACCA

GCCTCGCAGG

AATTTTTGGC

CAGCAAGATT

AGCTACGAGA

5461

CTCTTAAAAC

TTCGCTATCT

CATACTAGGA

TCGGCTGTTG

GGGGTGGCTA

CACAGCCAAA

5521

AAGACTTTTG

ATCAGTGGAA

AGATATGATA

CCGGACCTTA

GTGAATATAA

ATGGATTGTG

5581

CCTGACATTG

TGTGGGAAAT

TGATGAGTAT

ATCGATTTTG

AGAAAATTAG

AAAAGCCCTT

5641

CCTAGTTCAG

AAGACCTTGT

AAAGTTAGCA

CCAGACTTTG

ACAAGATTGT

TGAAAGCCTT

5701

AGCTTATTGA

AGGACTTTTT

TACCTCAGGT

CACAAATTGG

TTAGTGAAGT

CATAGGAGCT

5761

TCTGACCTAC

TTCTCTTGTT

AGGTTCTCCG

GAAGAAACGG

CGTTTAGAGC

AACAGATCGT

5821

GGATCTGAAA

GTGACAAGCA

TTTTAGAAAG

GGTCTGCTTG

GTGAGCTCAT

TCTCTTACAA

5881

CAACAAATTC

AAGAGCATGA

AGAGGAAGCG

CGCAGAGCCG

CTGGCCAATA

TAGCACGAGC

5941

TATGCCCAAC

AGAAGCGCAA

GGTGTCAGAC

AAAGAGAAAA

TTGACCAACT

TCAGGAAGAA

6001

CTTCTGCACA

CTCAGTTGAA

GTATCAGAGA

ATCTTGGAAC

GATTAGAAAA

GGAGAACAAA

6061

GAATTGAGAA

AATTAGTATT

GCAGAAAGAT

GACAAAGGCA

TTCATCATAG

AAAGCTTAAG

6121

AAATCTTTGA

TTGACATGTA

TTCTGAAGTT

CTTGATGTTC

TCTCTGATTA

TGATGCCAGT

6181

TATAATACGC

AAGATCATCT

GCCACGGGTT

GTTGTGGTTG

GAGATCAGAG

TGCTGGAAAG

6241

ACTAGTGTGT

TGGAAATGAT

TGCCCAAGCT

CGAATATTCC

CAAGAGGATC

TGGGGAGATG

6301

ATGACACGTT

CTCCAGTTAA

GGTGACTCTG

AGTGAAGGTC

CTCACCATGT

GGCCCTATTT

6361

AAAGATAGTT

CTCGGGAGTT

TGATCTTACC

AAAGAAGAAG

ATCTTGCAGC

ATTAAGACAT

6421

GAAATAGAAC

TTCGAATGAG

GAAAAATGTG

AAAGAAGGCT

GTACCGTTAG

CCCTGAGACC

6481

ATATCCTTAA

ATGTAAAAGG

CCCTGGACTA

CAGAGGATGG

TGCTTGTTGA

CTTACCAGGT

6541

GTGATTAATA

CTGTGACATC

AGGCATGGCT

CCTGACACAA

AGGAAACTAT

TTTCAGTATC

6601

AGCAAAGCTT

ACATGCAGAA

TCCTAATGCC

ATCATACTGT

GTATTCAAGA

TGGATCTGTG

6661

GATGCTGAAC

GCAGTATTGT

TACAGACTTG

GTCAGTCAAA

TGGACCCTCA

TGGAAGGAGA

6721

ACCATATTCG

TTTTGACCAA

AGTAGACCTG

GCAGAGAAAA

ATGTAGCCAG

TCCAAGCAGG

6781

ATTCAGCAGA

TAATTGAAGG

AAAGCTCTTC

CCAATGAAAG

CTTTAGGTTA

TTTTGCTGTT

6841

GTAACAGGAA

AAGGGAACAG

CTCTGAAAGC

ATTGAAGCTA

TAAGAGAATA

TGAAGAAGAG

6901

TTTTTTCAGA

ATTCAAAGCT

CCTAAAGACA

AGCATGCTAA

AGGCACACCA

AGTGACTACA

6961

AGAAATTTAA

GCCTTGCAGT

ATCAGACTGC

TTTTGGAAAA

TGGTACGAGA

GTCTGTTGAA

7021

CAACAGGCTG

ATAGTTTCAA

AGCAACACGT

TTTAACCTTG

AAACTGAATG

GAAGAATAAC

7081

TATCCTCGCC

TGCGGGAACT

TGACCGGAAT

GAACTATTTG

AAAAAGCTAA

AAATGAAATC

7141

CTTGATGAAG

TTATCAGTCT

GAGCCAGGTT

ACACCAAAAC

ATTGGGAGGA

AATCCTTCAA

7201

CAATCTTTGT

GGGAAAGAGT

ATCAACTCAT

GTGATTGAAA

ACATCTACCT

TCCAGCTGCG

7261

CAGACCATGA

ATTCAGGAAC

TTTTAACACC

ACAGTGGATA

TCAAGCTTAA

ACAGTGGACT

7321

GATAAACAAC

TTCCTAATAA

AGCAGTAGAG

GTTGCTTGGG

AGACCCTACA

AGAAGAATTT

7381

TCCCGCTTTA

TGACAGAACC

GAAAGGGAAA

GAGCATGATG

ACATATTTGA

TAAACTTAAA

7441

GAGGCTGTTA

AGGAAGAAAG

TATTAAACGA

CACAAGTGGA

ATGACTTTGC

GGAGGACAGC

7501

TTGAGGGTTA

TTCAACACAA

TGCTTTGGAA

GACCGATCCA

TATCTGATAA

ACAGCAATGG

7561

GATGCAGCTA

TTTATTTTAT

GGAAGAGGCT

CTGCAGGCTC

GTCTCAAGGA

TACTGAAAAT

7621

GCAATTGAAA

ACATGGTGGG

TCCAGACTGG

AAAAAGAGGT

GGTTATACTG

GAAGAATCGG

7681

ACCCAAGAAC

AGTGTGTTCA

CAATGAAACC

AAGAATGAAT

TGGAGAAGAT

GTTGAAATGT

7741

AATGAGGAGC

ACCCAGCTTA

TCTTGCAAGT

GATGAAATAA

CCACAGTCCG

GAAGAACCTT

7801

GAATCCCGAG

GAGTAGAAGT

AGATCCAAGC

TTGATTAAGG

ATACTTGGCA

TCAAGTTTAT

7861

AGAAGACATT

TTTTAAAAAC

AGCTCTAAAC

CATTGTAACC

TTTGTCGAAG

AGGTTTTTAT

7921

TACTACCAAA

GGCATTTTGT

AGATTCTGAG

TTGGAATGCA

ATGATGTGGT

CTTGTTTTGG

7981

CGTATACAGC

GCATGCTTGC

TATCACCGCA

AATACTTTAA

GGCAACAACT

TACAAATACT

8041

GAAGTTAGGC

GATTAGAGAA

AAATGTTAAA

GAGGTATTGG

AAGATTTTGC

TGAAGATGGT

8101

GAGAAGAAGA

TTAAATTGCT

TACTGGTAAA

CGCGTTCAAC

TGGCGGAAGA

CCTCAAGAAA

8161

GTTAGAGAAA

TTCAAGAAAA

ACTTGATGCT

TTCATTGAAG

CTCTTCATCA

GGAGAAACTC

8221

GAGCACCACC

ACCACCACCA

CTGAGATCCG

GCTGCTAACA

AAGCCCGAAA

GGAAGCTGAG

8281

TTGGCTGCTG

CCACCGCTGA

GCAATAACTA

GCATAACCCC

TTGGGGCCTC

TAAACGGGTC

8341

TTGAGGGGTT

TTTTGCTGAA

AGGAGGAACT

ATATCCGGAT

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