名称: | pSIN-EF1a-EIF3G(human)-FLAG-Puro |
---|---|
别称: | NM_003755.5;"eIF3-delta; EIF3-P42; eIF3-p44; EIF3S4" |
基因长: | 963bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pSIN-EF2-Puro |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGACCGCCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTGGA
AGGGCTAATT
CACTCCCAAA
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTAGC
AGAACTACAC
ACCAGGGCCA
GGGGTCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCCAGAT
AAGGTAGAAG
AGGCCAATAA
AGGAGAGAAC
ACCAGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTGTTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACGTGG
CCCGAGAGCT
GCATCCGGAG
TACTTCAAGA
ACTGCTGATA
TCGAGCTTGC
TACAAGGGAC
TTTCCGCTGG
GGACTTTCCA
GGGAGGCGTG
GCCTGGGCGG
GACTGGGGAG
TGGCGAGCCC
TCAGATCCTG
CATATAAGCA
GCTGCTTTTT
GCCTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGCTGAT
CTTCAGACCT
GGAGGAGGAG
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGCCA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAGGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
ACTTTGGATC
GATAAGCTTT
GCAAAGATGG
ATAAAGTTTT
AAACAGAGAG
GAATCTTTGC
AGCTAATGGA
CCTTCTAGGT
CTTGAAAGGA
GTGGGAATTG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GTAAGTGCCG
TGTGTGGTTC
CCGCGGGCCT
GGCCTCTTTA
CGGGTTATGG
CCCTTGCGTG
CCTTGAATTA
CTTCCACCTG
GCTGCAGTAC
GTGATTCTTG
ATCCCGAGCT
TCGGGTTGGA
AGTGGGTGGG
AGAGTTCGAG
GCCTTGCGCT
TAAGGAGCCC
CTTCGCCTCG
TGCTTGAGTT
GAGGCCTGGC
CTGGGCGCTG
GGGCCGCCGC
GTGCGAATCT
GGTGGCACCT
TCGCGCCTGT
CTCGCTGCTT
TCGATAAGTC
TCTAGCCATT
TAAAATTTTT
GATGACCTGC
TGCGACGCTT
TTTTTCTGGC
AAGATAGTCT
TGTAAATGCG
GGCCAAGATC
TGCACACTGG
TATTTCGGTT
TTTGGGGCCG
CGGGCGGCGA
CGGGGCCCGT
GCGTCCCAGC
GCACATGTTC
GGCGAGGCGG
GGCCTGCGAG
CGCGGCCACC
GAGAATCGGA
CGGGGGTAGT
CTCAAGCTGG
CCGGCCTGCT
CTGGTGCCTG
GCCTCGCGCC
GCCGTGTATC
GCCCCGCCCT
GGGCGGCAAG
GCTGGCCCGG
TCGGCACCAG
TTGCGTGAGC
GGAAAGATGG
CCGCTTCCCG
GCCCTGCTGC
AGGGAGCTCA
AAATGGAGGA
CGCGGCGCTC
GGGAGAGCGG
GCGGGTGAGT
CACCCACACA
AAGGAAAAGG
GCCTTTCCGT
CCTCAGCCGT
CGCTTCATGT
GACTCCACGG
AGTACCGGGC
GCCGTCCAGG
CACCTCGATT
AGTTCTCGAG
CTTTTGGAGT
ACGTCGTCTT
TAGGTTGGGG
GGAGGGGTTT
TATGCGATGG
AGTTTCCCCA
CACTGAGTGG
GTGGAGACTG
AAGTTAGGCC
AGCTTGGCAC
TTGATGTAAT
TCTCCTTGGA
ATTTGCCCTT
TTTGAGTTTG
GATCTTGGTT
CATTCTCAAG
CCTCAGACAG
TGGTTCAAAG
TTTTTTTCTT
CCATTTCAGG
TGTCGTGAGG
AATTGGGATC
CGCCACCATG
CCTACTGGAG
ACTTTGATTC
GAAGCCCAGT
TGGGCCGACC
AGGTGGAGGA
GGAGGGGGAG
GACGACAAAT
GTGTCACCAG
CGAGCTCCTC
AAGGGGATCC
CTCTGGCCAC
AGGTGACACC
AGCCCAGAGC
CAGAGCTACT
GCCGGGAGCT
CCACTGCCGC
CTCCCAAGGA
GGTCATCAAC
GGAAACATAA
AGACAGTGAC
AGAGTACAAG
ATAGATGAGG
ATGGCAAGAA
GTTCAAGATT
GTCCGCACCT
TCAGGATTGA
GACCCGGAAG
GCTTCAAAGG
CTGTCGCAAG
GAGGAAGAAC
TGGAAGAAGT
TCGGGAACTC
AGAGTTTGAC
CCCCCCGGAC
CCAATGTGGC
CACCACCACT
GTCAGTGACG
ATGTCTCTAT
GACGTTCATC
ACCAGCAAAG
AGGACCTGAA
CTGCCAGGAG
GAGGAGGACC
CTATGAACAA
ACTCAAGGGC
CAGAAGATCG
TGTCCTGCCG
CATCTGCAAG
GGCGACCACT
GGACCACCCG
CTGCCCCTAC
AAGGATACGC
TGGGGCCCAT
GCAGAAGGAG
CTGGCCGAGC
AGCTGGGCCT
GTCTACTGGC
GAGAAGGAGA
AGCTGCCGGG
AGAGCTAGAG
CCGGTGCAGG
CCACGCAGAA
CAAGACAGGG
AAGTATGTGC
CGCCGAGCCT
GCGCGACGGG
GCCAGCCGCC
GCGGGGAGTC
CATGCAGCCC
AACCGCAGAG
CCGACGACAA
CGCCACCATC
CGTGTCACCA
ACTTGTCAGA
GGACACGCGT
GAGACCGACC
TGCAGGAGCT
CTTCCGGCCT
TTCGGCTCCA
TCTCCCGCAT
CTACCTGGCT
AAGGACAAGA
CCACTGGCCA
ATCCAAGGGC
TTTGCCTTCA
TCAGCTTCCA
CCGCCGCGAG
GATGCTGCGC
GTGCCATTGC
CGGGGTGTCC
GGCTTTGGCT
ACGACCACCT
CATCCTCAAC
GTCGAGTGGG
CCAAGCCGTC
CACCAACGAC
TACAAGGACG
ACGATGACAA
GTAGGAATTC
TTCGAACATA
TGAAGCTTGC
TAGCACTAGT
GATCCGCATG
CATCTAGGGC
GGCCAATTCC
GCCCCTCTCC
CCCCCCCCCC
TCTCCCTCCC
CCCCCCCTAA
CGTTACTGGC
CGAAGCCGCT
TGGAATAAGG
CCGGTGTGCG
TTTGTCTATA
TGTTATTTTC
CACCATATTG
CCGTCTTTTG
GCAATGTGAG
GGCCCGGAAA
CCTGGCCCTG
TCTTCTTGAC
GAGCATTCCT
AGGGGTCTTT
CCCCTCTCGC
CAAAGGAATG
CAAGGTCTGT
TGAATGTCGT
GAAGGAAGCA
GTTCCTCTGG
AAGCTTCTTG
AAGACAAACA
ACGTCTGTAG
CGACCCTTTG
CAGGCAGCGG
AACCCCCCAC
CTGGCGACAG
GTGCCTCTGC
GGCCAAAAGC
CACGTGTATA
AGATACACCT
GCAAAGGCGG
CACAACCCCA
GTGCCACGTT
GTGAGTTGGA
TAGTTGTGGA
AAGAGTCAAA
TGGCTCTCCT
CAAGCGTATT
CAACAAGGGG
CTGAAGGATG
CCCAGAAGGT
ACCCCATTGT
ATGGGATCTG
ATCTGGGGCC
TCGGTGCACA
TGCTTTACAT
GTGTTTAGTC
GAGGTTAAAA
AAACGTCTAG
GCCCCCCGAA
CCACGGGGAC
GTGGTTTTCC
TTTGAAAAAC
ACGATGATAA
GCTTGCCACA
ACCCACAAGG
AGACGACCTT
CCATGACCGA
GTACAAGCCC
ACGGTGCGCC
TCGCCACCCG
CGACGACGTC
CCCCGGGCCG
TACGCACCCT
CGCCGCCGCG
TTCGCCGACT
ACCCCGCCAC
GCGCCACACC
GTCGACCCGG
ACCGCCACAT
CGAGCGGGTC
ACCGAGCTGC
AAGAACTCTT
CCTCACGCGC
GTCGGGCTCG
ACATCGGCAA
GGTGTGGGTC
GCGGACGACG
GCGCCGCGGT
GGCGGTCTGG
ACCACGCCGG
AGAGCGTCGA
AGCGGGGGCG
GTGTTCGCCG
AGATCGGCCC
GCGCATGGCC
GAGTTGAGCG
GTTCCCGGCT
GGCCGCGCAG
CAACAGATGG
AAGGCCTCCT
GGCGCCGCAC
CGGCCCAAGG
AGCCCGCGTG
GTTCCTGGCC
ACCGTCGGCG
TCTCGCCCGA
CCACCAGGGC
AAGGGTCTGG
GCAGCGCCGT
CGTGCTCCCC
GGAGTGGAGG
CGGCCGAGCG
CGCCGGGGTG
CCCGCCTTCC
TGGAGACCTC
CGCGCCCCGC
AACCTCCCCT
TCTACGAGCG
GCTCGGCTTC
ACCGTCACCG
CCGACGTCGA
GGTGCCCGAA
GGACCGCGCA
CCTGGTGCAT
GACCCGCAAG
CCCGGTGCCT
GATAATAGGC
GGCCGCTCGA
GACCTAGAAA
AACATGGAGC
AATCACAAGT
AGCAATACAG
CAGCTACCAA
TGCTGATTGT
GCCTGGCTAG
AAGCACAAGA
GGAGGAGGAG
GTGGGTTTTC
CAGTCACACC
TCAGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGACAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
AGTAGTTCAT
GTCATCTTAT
TATTCAGTAT
TTATAACTTG
CAAAGAAATG
AATATCAGAG
AGTGAGAGGC
CTTGACATTA
TAATAGATTT
AGCAGGAATT
GAACTAGGAG
TGGAGCACAC
AGGCAAAGTT
CTAGAGCTCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGGTG
GGGCAGGACA
GCAAGGGGGG
AGGATTGGGA
AGACAATAGC
AGGCATGCTG
GGGATGCGGT
GGGCTCTATG
GCTTCTGAGG
CGGAAAGAAC
CAGCTGGGGG
CGCGCCCCTC
GAGGCCGCCA
TGGTCATAGC
TGTTTGACGT
CAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ACGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTATTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTT
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
TGGCCTTTTG
CA