产品货号：P64586
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV-Tet-miniCMV-RNF216(human)-GFP-nanobody-mCherry-Puro |
|---|---|
| 别称: | NM_207111.4;CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN |
| 基因长: | 2772bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达,四环素诱导 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 红色 |
| 启动子: | Tet-miniCMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV-Tet-miniCMV-MCS-GFP-nanobody-mCherry-Puro |
| 正向引物: | PFLAG-CMV-F |
| 反向引物: | |
| 诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTCCCCAA
GTAGCAGTGA
ACTCCAAGCC
ACCATGGAAG
AGGGAAACAA
CAATGAAGAG
GTAATTCACT
TGAACAACTT
TCACTGCCAT
CGGGGACAAG
AGTGGATCAA
TCTCCGAGAT
GGGCCCATCA
CCATATCTGA
CTCCTCAGAT
GAGGAAAGGA
TTCCAATGCT
GGTCACCCCA
GCTCCTCAGC
AGCATGAAGA
AGAGGACCTG
GATGATGATG
TCATCCTGAC
AGAAACAAAT
AAACCTCAGA
GATCACGACC
CAATCTCATC
AAACCAGCTG
CCCAGTGGCA
AGATCTGAAA
AGGTTGGGAG
AAGAAAGGCC
TAAAAAGTCT
AGAGCAGCAT
TTGAATCAGA
TAAGAGCAGC
TATTTTTCAG
TGTGTAACAA
CCCATTGTTT
GATTCTGGGG
CACAGGATGA
TTCTGAGGAT
GACTACGGTG
AATTTCTGGA
TCTTGGGCCT
CCTGGAATCT
CTGAATTCAC
TAAGCCAAGT
GGCCAAACAG
AAAGAGAACC
CAAGCCTGGA
CCGAGTCATA
ACCAAGCAGC
AAATGACATT
GTCAACCCCA
GATCAGAGCA
GAAAGTCATC
ATCTTGGAAG
AAGGTAGCCT
TCTTTACACA
GAAAGCGATC
CTTTGGAAAC
TCAGAACCAG
TCATCCGAAG
ACTCAGAGAC
AGAGCTGTTA
TCAAATCTAG
GAGAGTCAGC
TGCTCTAGCA
GATGATCAGG
CCATCGAAGA
AGACTGCTGG
TTAGATCATC
CTTACTTCCA
GTCTCTGAAC
CAACAGCCCC
GTGAAATAAC
AAACCAGGTC
GTTCCTCAGG
AACGGCAGCC
TGAAGCAGAA
CTGGGCCGCT
TGTTGTTTCA
GCATGAATTC
CCAGGGCCCG
CTTTTCCAAG
GCCGGAACCC
CAGCAAGGTG
GGATTTCAGG
CCCCTCTTCT
CCTCAGCCTG
CCCATCCTCT
AGGAGAGTTT
GAAGACCAGC
AGTTAGCAAG
TGATGATGAA
GAGCCAGGTC
CAGCCTTTCC
AATGCAAGAA
TCTCAAGAGC
CCAATTTGGA
AAACATTTGG
GGGCAAGAAG
CTGCAGAGGT
AGATCAAGAG
CTCGTTGAAC
TACTAGTGAA
AGAAACGGAA
GCAAGATTTC
CAGATGTAGC
AAATGGGTTT
ATTGAGGAAA
TAATTCATTT
TAAGAATTAT
TATGATCTGA
ATGTACTTTG
TAATTTTCTT
CTGGAAAACC
CAGATTATCC
AAAGAGAGAA
GACAGAATCA
TTATAAATCC
CAGTAGCAGT
CTGCTGGCCA
GCCAAGATGA
GACAAAGTTG
CCTAAAATAG
ACTTTTTTGA
CTATTCTAAA
TTGACCCCTC
TTGACCAGCG
CTGCTTCATC
CAAGCTGCTG
ACCTCCTCAT
GGCCGACTTC
AAAGTGCTCA
GTAGTCAGGA
CATCAAGTGG
GCCCTGCACG
AGCTCAAAGG
ACACTATGCA
ATCACCCGAA
AGGCCTTGTC
TGATGCCATT
AAAAAATGGC
AGGAGCTGTC
ACCAGAAACC
AGTGGAAAAA
GGAAGAAGAG
AAAACAAATG
AACCAGTATT
CTTACATTGA
TTTCAAGTTT
GAACAAGGTG
ACATAAAAAT
AGAAAAGAGG
ATGTTCTTTC
TTGAAAATAA
GCGACGACAT
TGTAGGTCCT
ATGACCGACG
TGCTCTCCTT
CCAGCTGTGC
AACAAGAGCA
GGAGTTCTAT
GAGCAGAAAA
TCAAAGAGAT
GGCAGAGCAT
GAAGACTTTT
TGCTTGCCCT
ACAGATGAAT
GAAGAACAGT
ATCAAAAGGA
TGGCCAGCTG
ATTGAGTGTC
GCTGCTGCTA
TGGGGAATTT
CCATTCGAGG
AGCTGACGCA
GTGCGCAGAT
GCTCACTTGT
TCTGCAAAGA
GTGTCTCATC
AGATATGCCC
AAGAGGCAGT
CTTTGGATCT
GGAAAGTTGG
AGCTCAGCTG
CATGGAAGGC
AGCTGCACGT
GTTCGTTCCC
AACCAGTGAG
CTGGAGAAGG
TGCTCCCCCA
GACCATCCTG
TATAAGTACT
ATGAGCGAAA
AGCCGAGGAG
GAGGTTGCGG
CAGCCTACGC
CGACGAGCTT
GTCAGGTGCC
CGTCCTGTAG
CTTTCCGGCT
CTGTTGGACA
GTGATGTGAA
GAGGTTCAGC
TGTCCTAATC
CTCACTGCCG
AAAGGAAACC
TGTAGGAAGT
GTCAGGGACT
CTGGAAAGAA
CATAATGGCC
TCACCTGTGA
AGAGCTGGCT
GAAAAAGACG
ACATCAAGTA
CCGTACCTCT
ATTGAAGAAA
AAATGACTGC
TGCCCGCATT
AGAAAATGCC
ACAAGTGTGG
GACTGGCCTC
ATCAAATCTG
AAGGCTGCAA
CCGCATGTCT
TGCCGCTGTG
GTGCCCAGAT
GTGCTACCTC
TGTCGAGTTT
CTATTAATGG
ATATGACCAT
TTCTGCCAAC
ATCCCCGCTC
ACCAGGAGCC
CCTTGCCAGG
AGTGTTCAAG
ATGCTCTCTC
TGGACCGATC
CCACTGAAGA
TGATGAGAAG
CTTATTGAGG
AAATCCAGAA
GGAGGCTGAA
GAGGAACAGA
AAAGAAAGAA
TGGAGAGAAC
ACCTTCAAAC
GCATTGGACC
CCCGCTGGAG
AAGCCTGTGG
AGAAGGTGCA
GAGGGTGGAG
GCCCTCCCGA
GGCCCGTTCC
GCAGAACCTG
CCACAGCCAC
AGATGCCACC
CTATGCCTTC
GCGCACCCAC
CCTTCCCCCT
GCCTCCCGTG
CGGCCTGTGT
TCAACAACTT
CCCACTCAAC
ATGGGGCCTA
TCCCAGCCCC
GTACGTGCCC
CCTCTGCCCA
ACGTGCGGGT
CAACTATGAC
TTCGGTCCCA
TCCACATGCC
CCTGGAGCAC
AACCTGCCCA
TGCACTTTGG
CCCCCAGCCG
CGGCATCGCT
TCTACCCCTA
CGACGTGCCT
GACTACGCCG
GCGGCGGTGG
TTCAGGCGGT
GGCGGTTCCG
GTGGTGGGGG
ATCAGTCCAA
CTGGTGGAGT
CTGGTGGCGC
TTTGGTGCAG
CCAGGTGGCT
CTCTGCGTTT
GTCCTGTGCC
GCTTCTGGCT
TCCCAGTGAA
CCGCTATTCC
ATGCGCTGGT
ATCGCCAGGC
TCCAGGCAAA
GAGCGTGAGT
GGGTAGCCGG
TATGTCCAGC
GCGGGTGATC
GTAGCTCCTA
TGAAGACTCC
GTGAAGGGCC
GTTTCACCAT
CAGCCGTGAC
GATGCCCGTA
ACACGGTGTA
TCTGCAAATG
AACAGCTTGA
AACCTGAAGA
TACGGCCGTG
TATTACTGTA
ATGTGAACGT
GGGCTTCGAG
TATTGGGGCC
AAGGCACCCA
GGTCACCGTC
TCCAGCTAAG
ATCCTTGACT
TGCGGCCGCA
ACTCCCACCT
GCAACATGCG
TGACTGACTG
AGGCCGCGAC
TCTAGAGTCG
ACCTGCATCT
AGGCGCCGGA
ATTAGATCTC
TCGAGGTTAA
CGAATTCTAC
CGGGTAGGGG
AGGCGCTTTT
CCCAAGGCAG
TCTGGAGCAT
GCGCTTTAGC
AGCCCCGCTG
GGCACTTGGC
GCTACACAAG
TGGCCTCTGG
CCTCGCACAC
ATTCCACATC
CACCGGTAGG
CGCCAACCGG
CTCCGTTCTT
TGGTGGCCCC
TTCGCGCCAC
CTTCTACTCC
TCCCCTAGTC
AGGAAGTTCC
CCCCCGCCCC
GCAGCTCGCG
TCGTGCAGGA
CGTGACAAAT
GGAAGTAGCA
CGTCTCACTA
GTCTCGTGCA
GATGGACAGC
ACCGCTGAGC
AATGGAAGCG
GGTAGGCCTT
TGGGGCAGCG
GCCAATAGCA
GCTTTGCTCC
TTCGCTTTCT
GGGCTCAGAG
GCTGGGAAGG
GGTGGGTCCG
GGGGCGGGCT
CAGGGGCGGG
CTCAGGGGCG
GGGCGGGCGC
CCGAAGGTCC
TCCGGAGGCC
CGGCATTCTG
CACGCTTCAA
AAGCGCACGT
CTGCCGCGCT
GTTCTCCTCT
TCCTCATCTC
CGGGCCTTTC
GACCTGCAGC
CCAAGCTTAC
CATGGTGAGC
AAGGGCGAGG
AGGATAACAT
GGCCATCATC
AAGGAGTTCA
TGCGCTTCAA
GGTGCACATG
GAGGGCTCCG
TGAACGGCCA
CGAGTTCGAG
ATCGAGGGCG
AGGGCGAGGG
CCGCCCCTAC
GAGGGCACCC
AGACCGCCAA
GCTGAAGGTG
ACCAAGGGTG
GCCCCCTGCC
CTTCGCCTGG
GACATCCTGT
CCCCTCAGTT
CATGTACGGC
TCCAAGGCCT
ACGTGAAGCA
CCCCGCCGAC
ATCCCCGACT
ACTTGAAGCT
GTCCTTCCCC
GAGGGCTTCA
AGTGGGAGCG
CGTGATGAAC
TTCGAGGACG
GCGGCGTGGT
GACCGTGACC
CAGGACTCCT
CCCTGCAGGA
CGGCGAGTTC
ATCTACAAGG
TGAAGCTGCG
CGGCACCAAC
TTCCCCTCCG
ACGGCCCCGT
AATGCAGAAG
AAGACCATGG
GCTGGGAGGC
CTCCTCCGAG
CGGATGTACC
CCGAGGACGG
CGCCCTGAAG
GGCGAGATCA
AGCAGAGGCT
GAAGCTGAAG
GACGGCGGCC
ACTACGACGC
TGAGGTCAAG
ACCACCTACA
AGGCCAAGAA
GCCCGTGCAG
CTGCCCGGCG
CCTACAACGT
CAACATCAAG
TTGGACATCA
CCTCCCACAA
CGAGGACTAC
ACCATCGTGG
AACAGTACGA
ACGCGCCGAG
GGCCGCCACT
CCACCGGCGG
CATGGACGAG
CTGTACAAGG
CAACAAACTT
CTCTCTGCTG
AAACAAGCCG
GAGATGTCGA
AGAGAATCCT
GGACCGATGA
CCGAGTACAA
GCCCACGGTG
CGCCTCGCCA
CCCGCGACGA
CGTCCCCAGG
GCCGTACGCA
CCCTCGCCGC
CGCGTTCGCC
GACTACCCCG
CCACGCGCCA
CACCGTCGAT
CCGGACCGCC
ACATCGAGCG
GGTCACCGAG
CTGCAAGAAC
TCTTCCTCAC
GCGCGTCGGG
CTCGACATCG
GCAAGGTGTG
GGTCGCGGAC
GACGGCGCCG
CGGTGGCGGT
CTGGACCACG
CCGGAGAGCG
TCGAAGCGGG
GGCGGTGTTC
GCCGAGATCG
GCCCGCGCAT
GGCCGAGTTG
AGCGGTTCCC
GGCTGGCCGC
GCAGCAACAG
ATGGAAGGCC
TCCTGGCGCC
GCACCGGCCC
AAGGAGCCCG
CGTGGTTCCT
GGCCACCGTC
GGCGTCTCGC
CCGACCACCA
GGGCAAGGGT
CTGGGCAGCG
CCGTCGTGCT
CCCCGGAGTG
GAGGCGGCCG
AGCGCGCCGG
GGTGCCCGCC
TTCCTGGAGA
CCTCCGCGCC
CCGCAACCTC
CCCTTCTACG
AGCGGCTCGG
CTTCACCGTC
ACCGCCGACG
TCGAGGTGCC
CGAAGGACCG
CGCACCTGGT
GCATGACCCG
CAAGCCCGGT
GCCTGATCTT
ATCGATAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AATCATCGTC
CTTTCCTTGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCATCGA
TACCGTCGAC
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATCTGCTTTT
TGCTTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGCATCTAGA
ATTAATTCCG
TGTATTCTAT
AGTGTCACCT
AAATCGTATG
TGTATGATAC
ATAAGGTTAT
GTATTAATTG
TAGCCGCGTT
CTAACGACAA
TATGTACAAG
CCTAATTGTG
TAGCATCTGG
CTTACTGAAG
CAGACCCTAT
CATCTCTCTC
GTAAACTGCC
GTCAGAGTCG
GTTTGGTTGG
ACGAACCTTC
TGAGTTTCTG
GTAACGCCGT
CCCGCACCCG
GAAATGGTCA
GCGAACCAAT
CAGCAGGGTC
ATCGCTAGCC
AGATCCTCTA
CGCCGGACGC
ATCGTGGCCG
GCATCACCGG
CGCCACAGGT
GCGGTTGCTG
GCGCCTATAT
CGCCGACATC
ACCGATGGGG
AAGATCGGGC
TCGCCACTTC
GGGCTCATGA
GCGCTTGTTT
CGGCGTGGGT
ATGGTGGCAG
GCCCCGTGGC
CGGGGGACTG
TTGGGCGCCA
TCTCCTTGCA
TGCACCATTC
CTTGCGGCGG
CGGTGCTCAA
CGGCCTCAAC
CTACTACTGG
GCTGCTTCCT
AATGCAGGAG
TCGCATAAGG
GAGAGCGTCG
AATGGTGCAC
TCTCAGTACA
ATCTAGCTCT
GATGCCGCAT
AGTTAAGCCA
GCCCCGACAC
CCGCCAACAC
CCGCTGACGC
GCCCTGACGG
GCTTGTCTGC
TCCCGGCATC
CGCTTACAGA
CAAGCTGTGA
CCGTCTCCGG
GAGCTGCATG
TGTCAGAGGT
TTTCACCGTC
ATCACCGAAA
CGCGCGAGAC
GAAAGGGCCT
CGTGATACGC
CTATTTTTAT
AGGTTAATGT
CATGATAATA
ATGGTTTCTT
AGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
GACAATAACC
CTGATAAATG
CTTCAATAAT
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
ATTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GTAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGGTCTCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTCCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCCCAATAC
GCAAACCGCC
TCTCCCCGCG
CGTTGGCCGA
TTCATTAATG
CAGCTGTGGA
ATGTGTGTCA
GTTAGGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCAGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAT
AGTCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CGGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGCTTTTGCA
AAAAGCTTGG
ACACAAGACA
GGCTTGCGAG
ATATGTTTGA
GAATACCACT
TTATCCCGCG
TCAGGGAGAG
GCAGTGCGTA
AAAAGACGCG
GACTCATGTG
AAATACTGGT
TTTTAGTGCG
CCAGATCTCT
ATAATCTCGC
GCAACCTATT
TTCCCCTCGA
ACACTTTTTA
AGCCGTAGAT
AAACAGGCTG
GGACACTTCA
CATGAGCGAA
AAATACATCG
TCACCTGGGA
CATGTTGCAG
ATCCATGCAC
GTAAACTCGC
AAGCCGACTG
ATGCCTTCTG
AACAATGGAA
AGGCATTATT
GCCGTAAGCC
GTGGCGGTCT
GTACCGGGTG
CGTTACTGGC
GCGTGAACTG
GGTATTCGTC
ATGTCGATAC
CGTTTGTATT
TCCAGCTACG
ATCACGACAA
CCAGCGCGAG
CTTAAAGTGC
TGAAACGCGC
AGAAGGCGAT
GGCGAAGGCT
TCATCGTTAT
TGATGACCTG
GTGGATACCG
GTGGTACTGC
GGTTGCGATT
CGTGAAATGT
ATCCAAAAGC
GCACTTTGTC
ACCATCTTCG
CAAAACCGGC
TGGTCGTCCG
CTGGTTGATG
ACTATGTTGT
TGATATCCCG
CAAGATACCT
GGATTGAACA
GCCGTGGGAT
ATGGGCGTCG
TATTCGTCCC
GCCAATCTCC
GGTCGCTAAT
CTTTTCAACG
CCTGGCACTG
CCGGGCGTTG
TTCTTTTTAA
CTTCAGGCGG
GTTACAATAG
TTTCCAGTAA
GTATTCTGGA
GGCTGCATCC
ATGACACAGG
CAAACCTGAG
CGAAACCCTG
TTCAAACCCC
GCTTTAAACA
TCCTGAAACC
TCGACGCTAG
TCCGCCGCTT
TAATCACGGC
GCACAACCGC
CTGTGCAGTC
GGCCCTTGAT
GGTAAAACCA
TCCCTCACTG
GTATCGCATG
ATTAACCGTC
TGATGTGGAT
CTGGCGCGGC
ATTGACCCAC
GCGAAATCCT
CGACGTCCAG
GCACGTATTG
TGATGAGCGA
TGCCGAACGT
ACCGACGATG
ATTTATACGA
TACGGTGATT
GGCTACCGTG
GCGGCAACTG
GATTTATGAG
TGGGCCCCGG
ATCTTTGTGA
AGGAACCTTA
CTTCTGTGGT
GTGACATAAT
TGGACAAACT
ACCTACAGAG
ATTTAAAGCT
CTAAGGTAAA
TATAAAATTT
TTAAGTGTAT
AATGTGTTAA
ACTACTGATT
CTAATTGTTT
GTGTATTTTA
GATTCCAACC
TATGGAACTG
ATGAATGGGA
GCAGTGGTGG
AATGCCTTTA
ATGAGGAAAA
CCTGTTTTGC
TCAGAAGAAA
TGCCATCTAG
TGATGATGAG
GCTACTGCTG
ACTCTCAACA
TTCTACTCCT
CCAAAAAAGA
AGAGAAAGGT
AGAAGACCCC
AAGGACTTTC
CTTCAGAATT
GCTAAGTTTT
TTGAGTCATG
CTGTGTTTAG
TAATAGAACT
CTTGCTTGCT
TTGCTATTTA
CACCACAAAG
GAAAAAGCTG
CACTGCTATA
CAAGAAAATT
ATGGAAAAAT
ATTCTGTAAC
CTTTATAAGT
AGGCATAACA
GTTATAATCA
TAACATACTG
TTTTTTCTTA
CTCCACACAG
GCATAGAGTG
TCTGCTATTA
ATAACTATGC
TCAAAAATTG
TGTACCTTTA
GCTTTTTAAT
TTGTAAAGGG
GTTAATAAGG
AATATTTGAT
GTATAGTGCC
TTGACTAGAG
ATCATAATCA
GCCATACCAC
ATTTGTAGAG
GTTTTACTTG
CTTTAAAAAA
CCTCCCACAC
CTCCCCCTGA
ACCTGAAACA
TAAAATGAAT
GCAATTGTTG
TTGTTAACTT
GTTTATTGCA
GCTTATAATG
GTTACAAATA
AAGCAATAGC
ATCACAAATT
TCACAAATAA
AGCATTTTTT
TCACTGCATT
CTAGTTGTGG
TTTGTCCAAA
CTCATCAATG
TATCTTATCA
TGTCTGGATC
AACTGGATAA
CTCAAGCTAA
CCAAAATCAT
CCCAAACTTC
CCACCCCATA
CCCTATTACC
ACTGCCAATT
ACCTAGTGGT
TTCATTTACT
CTAAACCTGT
GATTCCTCTG
AATTATTTTC
ATTTTAAAGA
AATTGTATTT
GTTAAATATG
TACTACAAAC
TTAGTAGTTG
GAAGGGCTAA
TTCACTCCCA
AAGAAGACAA
GATATCCTTG
ATCTGTGGAT
CTACCACACA
CAAGGCTACT
TCCCTGATTA
GCAGAACTAC
ACACCAGGGC
CAGGGGTCAG
ATATCCACTG
ACCTTTGGAT
GGTGCTACAA
GCTAGTACCA
GTTGAGCCAG
ATAAGGTAGA
AGAGGCCAAT
AAAGGAGAGA
ACACCAGCTT
GTTACACCCT
GTGAGCCTGC
ATGGGATGGA
TGACCCGGAG
AGAGAAGTGT
TAGAGTGGAG
GTTTGACAGC
CGCCTAGCAT
TTCATCACGT
GGCCCGAGAG
CTGCATCCGG
AGTACTTCAA
GAACTGCTGA
TATCGAGCTT
GCTACAAGGG
ACTTTCCGCT
GGGGACTTTC
CAGGGAGGCG
TGGCCTGGGC
GGGACTGGGG
AGTGGCGAGC
CCTCAGATCC
TGCATATAAG
CAGCTGCTTT
TTGCCTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTGGCGCC
CGAACAGGGA
CTTGAAAGCG
AAAGGGAAAC
CAGAGGAGCT
CTCTCGACGC
AGGACTCGGC
TTGCTGAAGC
GCGCACGGCA
AGAGGCGAGG
GGCGGCGACT
GGTGAGTACG
CCAAAAATTT
TGACTAGCGG
AGGCTAGAAG
GAGAGAGATG
GGTGCGAGAG
CGTCAGTATT
AAGCGGGGGA
GAATTAGATC
GCGATGGGAA
AAAATTCGGT
TAAGGCCAGG
GGGAAAGAAA
AAATATAAAT
TAAAACATAT
AGTATGGGCA
AGCAGGGAGC
TAGAACGATT
CGCAGTTAAT
CCTGGCCTGT
TAGAAACATC
AGAAGGCTGT
AGACAAATAC
TGGGACAGCT
ACAACCATCC
CTTCAGACAG
GATCAGAAGA
ACTTAGATCA
TTATATAATA
CAGTAGCAAC
CCTCTATTGT
GTGCATCAAA
GGATAGAGAT
AAAAGACACC
AAGGAAGCTT
TAGACAAGAT
AGAGGAAGAG
CAAAACAAAA
GTAAGACCAC
CGCACAGCAA
GCGGCCGGTG
ATCTTCAGAC
CTGGACGATA
TATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCTCGA
CGGTCG
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