名称: | pLV-Tet-miniCMV-IPP(human)-GFP-nanobody-mCherry-Puro |
---|---|
别称: | NM_005897.3;KLHL27 |
基因长: | 1752bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 红色 |
启动子: | Tet-miniCMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV-Tet-miniCMV-MCS-GFP-nanobody-mCherry-Puro |
正向引物: | PFLAG-CMV-F |
反向引物: | |
诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTCAAGTT
GCCGCAGTGA
ACTCCAAGCC
ACCATGGCTA
ATGAGGACTG
TCCCAAGGCT
GCTGATAGTC
CTTTTTCATC
AGATAAACAT
GCCCAACTCA
TCTTGGCCCA
AATCAATAAG
ATGAGAAATG
GACAGCATTT
CTGTGATGTG
CAGCTGCAAG
TTGGACAGGA
AAGTTTTAAA
GCTCATCGGC
TGGTTTTGGC
TGCCAGCAGT
CCTTACTTTG
CAGCTTTGTT
CACTGGAGGA
ATGAAAGAGT
CCTCAAAAGA
TGTTGTACCG
ATTCTAGGAA
TTGAAGCAGG
AATCTTTCAG
ATACTTCTAG
ATTTCATTTA
CACAGGTATA
GTGAACATAG
GTGTGAATAA
TGTCCAGGAG
TTGATTATTG
CAGCAGACAT
GCTACAGTTG
ACTGAAGTTG
TTCATCTTTG
CTGTGAATTT
CTGAAAGGAC
AAATTGATCC
ACTGAACTGC
ATTGGAATTT
TTCAGTTCTC
TGAGCAAATT
GCCTGCCATG
ATCTCTTGGA
ATTCTCAGAA
AACTACATTC
ATGTCCATTT
CTTGGAGGTT
CATAGTGGAG
AAGAGTTCCT
GGCACTTACG
AAAGATCAGC
TGATCAAAAT
TTTGCGAAGT
GAAGAGCTTA
GCATTGAGGA
TGAATACCAG
GTCTTCTTAG
CTGCAATGCA
ATGGATTCTG
AAAGATTTGG
GAAAAAGAAG
AAAACATGTG
GTGGAAGTGC
TAGACCCAAT
TCGATTCCCT
TTATTACCTC
CTCAGAGACT
TTTAAAGTAT
ATAGAAGGAG
TATCCGATTT
TAATCTTCGT
GTTGCATTGC
AAACACTTCT
GAAAGAGTAC
TGTGAAGTAT
GCAAATCTCC
CAAAGAGAAC
AAGTTTTGTA
GTTTTCTGCA
GACATCTAAG
GTTCGACCTC
GGAAGAAAGC
AAGAAAGTAC
CTGTATGCAG
TAGGTGGATA
TACTCGGTTG
CAGGGGGGTC
GCTGGAGTGA
TAGCAGAGCC
CTCAGCTGTG
TAGAACGTTT
TGACACCTTT
AGCCAGTACT
GGACCACTGT
GTCTTCACTT
CATCAGGCTC
GAAGTGGGCT
GGGAGTAACA
GTTCTGGGAG
GGATGGTCTA
CGCTATTGGA
GGTGAAAAGG
ATTCAATGAT
TTTTGATTGT
ACTGAATGCT
ATGATCCAGT
TACTAAACAG
TGGACAACTG
TAGCTTCGAT
GAATCATCCC
CGCTGCGGCT
TAGGAGTGTG
TGTGTGTTAT
GGGGCTATCT
ATGCTTTGGG
TGGATGGGTT
GGAGCTGAGA
TAGGGAACAC
CATTGAACGA
TTTGATCCTG
ATGAAAATAA
ATGGGAAGTA
GTTGGTAACA
TGGCTGTGTC
ACGCTACTAC
TTTGGGTGCT
GTGAAATGCA
AGGTTTAATT
TATGTAATTG
GGGGCATCAG
CAATGAAGGA
ATAGAACTTC
GTTCTTTTGA
AGTCTATGAT
CCACTTTCTA
AGCGTTGGTC
TCCACTTCCT
CCAATGGGAA
CCAGGAGAGC
ATATCTTGGT
GTGGCTGCAC
TCAATGACTG
CATCTATTCT
GTTGGAGGAT
GGAATGAGAC
CCAAGATGCT
CTTCATACTG
TAGAAAAATA
TTCCTTTGAA
GAGGAAAAGT
GGGTTGAAGT
TGCCTCAATG
AAAGTGCCTA
GAGCAGGCAT
GTGTGTTGTG
GCAGTCAATG
GTCTTCTGTA
TGTTTCTGGA
GGTCGATCTT
CCAGCCATGA
TTTTTTGGCT
CCAGGTACCT
TGGACTCAGT
TGAAGTTTAT
AACCCTCATT
CAGATACATG
GACAGAAATT
GGTAACATGA
TCACCAGTCG
TTGTGAAGGG
GGCGTTGCTG
TGCTATACCC
CTACGACGTG
CCTGACTACG
CCGGCGGCGG
TGGTTCAGGC
GGTGGCGGTT
CCGGTGGTGG
GGGATCAGTC
CAACTGGTGG
AGTCTGGTGG
CGCTTTGGTG
CAGCCAGGTG
GCTCTCTGCG
TTTGTCCTGT
GCCGCTTCTG
GCTTCCCAGT
GAACCGCTAT
TCCATGCGCT
GGTATCGCCA
GGCTCCAGGC
AAAGAGCGTG
AGTGGGTAGC
CGGTATGTCC
AGCGCGGGTG
ATCGTAGCTC
CTATGAAGAC
TCCGTGAAGG
GCCGTTTCAC
CATCAGCCGT
GACGATGCCC
GTAACACGGT
GTATCTGCAA
ATGAACAGCT
TGAAACCTGA
AGATACGGCC
GTGTATTACT
GTAATGTGAA
CGTGGGCTTC
GAGTATTGGG
GCCAAGGCAC
CCAGGTCACC
GTCTCCAGCT
AAGATCCTTG
ACTTGCGGCC
GCAACTCCCA
CCTGCAACAT
GCGTGACTGA
CTGAGGCCGC
GACTCTAGAG
TCGACCTGCA
TCTAGGCGCC
GGAATTAGAT
CTCTCGAGGT
TAACGAATTC
TACCGGGTAG
GGGAGGCGCT
TTTCCCAAGG
CAGTCTGGAG
CATGCGCTTT
AGCAGCCCCG
CTGGGCACTT
GGCGCTACAC
AAGTGGCCTC
TGGCCTCGCA
CACATTCCAC
ATCCACCGGT
AGGCGCCAAC
CGGCTCCGTT
CTTTGGTGGC
CCCTTCGCGC
CACCTTCTAC
TCCTCCCCTA
GTCAGGAAGT
TCCCCCCCGC
CCCGCAGCTC
GCGTCGTGCA
GGACGTGACA
AATGGAAGTA
GCACGTCTCA
CTAGTCTCGT
GCAGATGGAC
AGCACCGCTG
AGCAATGGAA
GCGGGTAGGC
CTTTGGGGCA
GCGGCCAATA
GCAGCTTTGC
TCCTTCGCTT
TCTGGGCTCA
GAGGCTGGGA
AGGGGTGGGT
CCGGGGGCGG
GCTCAGGGGC
GGGCTCAGGG
GCGGGGCGGG
CGCCCGAAGG
TCCTCCGGAG
GCCCGGCATT
CTGCACGCTT
CAAAAGCGCA
CGTCTGCCGC
GCTGTTCTCC
TCTTCCTCAT
CTCCGGGCCT
TTCGACCTGC
AGCCCAAGCT
TACCATGGTG
AGCAAGGGCG
AGGAGGATAA
CATGGCCATC
ATCAAGGAGT
TCATGCGCTT
CAAGGTGCAC
ATGGAGGGCT
CCGTGAACGG
CCACGAGTTC
GAGATCGAGG
GCGAGGGCGA
GGGCCGCCCC
TACGAGGGCA
CCCAGACCGC
CAAGCTGAAG
GTGACCAAGG
GTGGCCCCCT
GCCCTTCGCC
TGGGACATCC
TGTCCCCTCA
GTTCATGTAC
GGCTCCAAGG
CCTACGTGAA
GCACCCCGCC
GACATCCCCG
ACTACTTGAA
GCTGTCCTTC
CCCGAGGGCT
TCAAGTGGGA
GCGCGTGATG
AACTTCGAGG
ACGGCGGCGT
GGTGACCGTG
ACCCAGGACT
CCTCCCTGCA
GGACGGCGAG
TTCATCTACA
AGGTGAAGCT
GCGCGGCACC
AACTTCCCCT
CCGACGGCCC
CGTAATGCAG
AAGAAGACCA
TGGGCTGGGA
GGCCTCCTCC
GAGCGGATGT
ACCCCGAGGA
CGGCGCCCTG
AAGGGCGAGA
TCAAGCAGAG
GCTGAAGCTG
AAGGACGGCG
GCCACTACGA
CGCTGAGGTC
AAGACCACCT
ACAAGGCCAA
GAAGCCCGTG
CAGCTGCCCG
GCGCCTACAA
CGTCAACATC
AAGTTGGACA
TCACCTCCCA
CAACGAGGAC
TACACCATCG
TGGAACAGTA
CGAACGCGCC
GAGGGCCGCC
ACTCCACCGG
CGGCATGGAC
GAGCTGTACA
AGGCAACAAA
CTTCTCTCTG
CTGAAACAAG
CCGGAGATGT
CGAAGAGAAT
CCTGGACCGA
TGACCGAGTA
CAAGCCCACG
GTGCGCCTCG
CCACCCGCGA
CGACGTCCCC
AGGGCCGTAC
GCACCCTCGC
CGCCGCGTTC
GCCGACTACC
CCGCCACGCG
CCACACCGTC
GATCCGGACC
GCCACATCGA
GCGGGTCACC
GAGCTGCAAG
AACTCTTCCT
CACGCGCGTC
GGGCTCGACA
TCGGCAAGGT
GTGGGTCGCG
GACGACGGCG
CCGCGGTGGC
GGTCTGGACC
ACGCCGGAGA
GCGTCGAAGC
GGGGGCGGTG
TTCGCCGAGA
TCGGCCCGCG
CATGGCCGAG
TTGAGCGGTT
CCCGGCTGGC
CGCGCAGCAA
CAGATGGAAG
GCCTCCTGGC
GCCGCACCGG
CCCAAGGAGC
CCGCGTGGTT
CCTGGCCACC
GTCGGCGTCT
CGCCCGACCA
CCAGGGCAAG
GGTCTGGGCA
GCGCCGTCGT
GCTCCCCGGA
GTGGAGGCGG
CCGAGCGCGC
CGGGGTGCCC
GCCTTCCTGG
AGACCTCCGC
GCCCCGCAAC
CTCCCCTTCT
ACGAGCGGCT
CGGCTTCACC
GTCACCGCCG
ACGTCGAGGT
GCCCGAAGGA
CCGCGCACCT
GGTGCATGAC
CCGCAAGCCC
GGTGCCTGAT
CTTATCGATA
ATCAACCTCT
GGATTACAAA
ATTTGTGAAA
GATTGACTGG
TATTCTTAAC
TATGTTGCTC
CTTTTACGCT
ATGTGGATAC
GCTGCTTTAA
TGCCTTTGTA
TCATGCTATT
GCTTCCCGTA
TGGCTTTCAT
TTTCTCCTCC
TTGTATAAAT
CCTGGTTGCT
GTCTCTTTAT
GAGGAGTTGT
GGCCCGTTGT
CAGGCAACGT
GGCGTGGTGT
GCACTGTGTT
TGCTGACGCA
ACCCCCACTG
GTTGGGGCAT
TGCCACCACC
TGTCAGCTCC
TTTCCGGGAC
TTTCGCTTTC
CCCCTCCCTA
TTGCCACGGC
GGAACTCATC
GCCGCCTGCC
TTGCCCGCTG
CTGGACAGGG
GCTCGGCTGT
TGGGCACTGA
CAATTCCGTG
GTGTTGTCGG
GGAAATCATC
GTCCTTTCCT
TGGCTGCTCG
CCTGTGTTGC
CACCTGGATT
CTGCGCGGGA
CGTCCTTCTG
CTACGTCCCT
TCGGCCCTCA
ATCCAGCGGA
CCTTCCTTCC
CGCGGCCTGC
TGCCGGCTCT
GCGGCCTCTT
CCGCGTCTTC
GCCTTCGCCC
TCAGACGAGT
CGGATCTCCC
TTTGGGCCGC
CTCCCCGCAT
CGATACCGTC
GACCAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAC
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGCATCT
AGAATTAATT
CCGTGTATTC
TATAGTGTCA
CCTAAATCGT
ATGTGTATGA
TACATAAGGT
TATGTATTAA
TTGTAGCCGC
GTTCTAACGA
CAATATGTAC
AAGCCTAATT
GTGTAGCATC
TGGCTTACTG
AAGCAGACCC
TATCATCTCT
CTCGTAAACT
GCCGTCAGAG
TCGGTTTGGT
TGGACGAACC
TTCTGAGTTT
CTGGTAACGC
CGTCCCGCAC
CCGGAAATGG
TCAGCGAACC
AATCAGCAGG
GTCATCGCTA
GCCAGATCCT
CTACGCCGGA
CGCATCGTGG
CCGGCATCAC
CGGCGCCACA
GGTGCGGTTG
CTGGCGCCTA
TATCGCCGAC
ATCACCGATG
GGGAAGATCG
GGCTCGCCAC
TTCGGGCTCA
TGAGCGCTTG
TTTCGGCGTG
GGTATGGTGG
CAGGCCCCGT
GGCCGGGGGA
CTGTTGGGCG
CCATCTCCTT
GCATGCACCA
TTCCTTGCGG
CGGCGGTGCT
CAACGGCCTC
AACCTACTAC
TGGGCTGCTT
CCTAATGCAG
GAGTCGCATA
AGGGAGAGCG
TCGAATGGTG
CACTCTCAGT
ACAATCTAGC
TCTGATGCCG
CATAGTTAAG
CCAGCCCCGA
CACCCGCCAA
CACCCGCTGA
CGCGCCCTGA
CGGGCTTGTC
TGCTCCCGGC
ATCCGCTTAC
AGACAAGCTG
TGACCGTCTC
CGGGAGCTGC
ATGTGTCAGA
GGTTTTCACC
GTCATCACCG
AAACGCGCGA
GACGAAAGGG
CCTCGTGATA
CGCCTATTTT
TATAGGTTAA
TGTCATGATA
ATAATGGTTT
CTTAGACGTC
AGGTGGCACT
TTTCGGGGAA
ATGTGCGCGG
AACCCCTATT
TGTTTATTTT
TCTAAATACA
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTT
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACGATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGGTCT
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ACTGTCAGAC
CAAGTTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTCC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCCAA
TACGCAAACC
GCCTCTCCCC
GCGCGTTGGC
CGATTCATTA
ATGCAGCTGT
GGAATGTGTG
TCAGTTAGGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
TGGACACAAG
ACAGGCTTGC
GAGATATGTT
TGAGAATACC
ACTTTATCCC
GCGTCAGGGA
GAGGCAGTGC
GTAAAAAGAC
GCGGACTCAT
GTGAAATACT
GGTTTTTAGT
GCGCCAGATC
TCTATAATCT
CGCGCAACCT
ATTTTCCCCT
CGAACACTTT
TTAAGCCGTA
GATAAACAGG
CTGGGACACT
TCACATGAGC
GAAAAATACA
TCGTCACCTG
GGACATGTTG
CAGATCCATG
CACGTAAACT
CGCAAGCCGA
CTGATGCCTT
CTGAACAATG
GAAAGGCATT
ATTGCCGTAA
GCCGTGGCGG
TCTGTACCGG
GTGCGTTACT
GGCGCGTGAA
CTGGGTATTC
GTCATGTCGA
TACCGTTTGT
ATTTCCAGCT
ACGATCACGA
CAACCAGCGC
GAGCTTAAAG
TGCTGAAACG
CGCAGAAGGC
GATGGCGAAG
GCTTCATCGT
TATTGATGAC
CTGGTGGATA
CCGGTGGTAC
TGCGGTTGCG
ATTCGTGAAA
TGTATCCAAA
AGCGCACTTT
GTCACCATCT
TCGCAAAACC
GGCTGGTCGT
CCGCTGGTTG
ATGACTATGT
TGTTGATATC
CCGCAAGATA
CCTGGATTGA
ACAGCCGTGG
GATATGGGCG
TCGTATTCGT
CCCGCCAATC
TCCGGTCGCT
AATCTTTTCA
ACGCCTGGCA
CTGCCGGGCG
TTGTTCTTTT
TAACTTCAGG
CGGGTTACAA
TAGTTTCCAG
TAAGTATTCT
GGAGGCTGCA
TCCATGACAC
AGGCAAACCT
GAGCGAAACC
CTGTTCAAAC
CCCGCTTTAA
ACATCCTGAA
ACCTCGACGC
TAGTCCGCCG
CTTTAATCAC
GGCGCACAAC
CGCCTGTGCA
GTCGGCCCTT
GATGGTAAAA
CCATCCCTCA
CTGGTATCGC
ATGATTAACC
GTCTGATGTG
GATCTGGCGC
GGCATTGACC
CACGCGAAAT
CCTCGACGTC
CAGGCACGTA
TTGTGATGAG
CGATGCCGAA
CGTACCGACG
ATGATTTATA
CGATACGGTG
ATTGGCTACC
GTGGCGGCAA
CTGGATTTAT
GAGTGGGCCC
CGGATCTTTG
TGAAGGAACC
TTACTTCTGT
GGTGTGACAT
AATTGGACAA
ACTACCTACA
GAGATTTAAA
GCTCTAAGGT
AAATATAAAA
TTTTTAAGTG
TATAATGTGT
TAAACTACTG
ATTCTAATTG
TTTGTGTATT
TTAGATTCCA
ACCTATGGAA
CTGATGAATG
GGAGCAGTGG
TGGAATGCCT
TTAATGAGGA
AAACCTGTTT
TGCTCAGAAG
AAATGCCATC
TAGTGATGAT
GAGGCTACTG
CTGACTCTCA
ACATTCTACT
CCTCCAAAAA
AGAAGAGAAA
GGTAGAAGAC
CCCAAGGACT
TTCCTTCAGA
ATTGCTAAGT
TTTTTGAGTC
ATGCTGTGTT
TAGTAATAGA
ACTCTTGCTT
GCTTTGCTAT
TTACACCACA
AAGGAAAAAG
CTGCACTGCT
ATACAAGAAA
ATTATGGAAA
AATATTCTGT
AACCTTTATA
AGTAGGCATA
ACAGTTATAA
TCATAACATA
CTGTTTTTTC
TTACTCCACA
CAGGCATAGA
GTGTCTGCTA
TTAATAACTA
TGCTCAAAAA
TTGTGTACCT
TTAGCTTTTT
AATTTGTAAA
GGGGTTAATA
AGGAATATTT
GATGTATAGT
GCCTTGACTA
GAGATCATAA
TCAGCCATAC
CACATTTGTA
GAGGTTTTAC
TTGCTTTAAA
AAACCTCCCA
CACCTCCCCC
TGAACCTGAA
ACATAAAATG
AATGCAATTG
TTGTTGTTAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
ATCAACTGGA
TAACTCAAGC
TAACCAAAAT
CATCCCAAAC
TTCCCACCCC
ATACCCTATT
ACCACTGCCA
ATTACCTAGT
GGTTTCATTT
ACTCTAAACC
TGTGATTCCT
CTGAATTATT
TTCATTTTAA
AGAAATTGTA
TTTGTTAAAT
ATGTACTACA
AACTTAGTAG
TTGGAAGGGC
TAATTCACTC
CCAAAGAAGA
CAAGATATCC
TTGATCTGTG
GATCTACCAC
ACACAAGGCT
ACTTCCCTGA
TTAGCAGAAC
TACACACCAG
GGCCAGGGGT
CAGATATCCA
CTGACCTTTG
GATGGTGCTA
CAAGCTAGTA
CCAGTTGAGC
CAGATAAGGT
AGAAGAGGCC
AATAAAGGAG
AGAACACCAG
CTTGTTACAC
CCTGTGAGCC
TGCATGGGAT
GGATGACCCG
GAGAGAGAAG
TGTTAGAGTG
GAGGTTTGAC
AGCCGCCTAG
CATTTCATCA
CGTGGCCCGA
GAGCTGCATC
CGGAGTACTT
CAAGAACTGC
TGATATCGAG
CTTGCTACAA
GGGACTTTCC
GCTGGGGACT
TTCCAGGGAG
GCGTGGCCTG
GGCGGGACTG
GGGAGTGGCG
AGCCCTCAGA
TCCTGCATAT
AAGCAGCTGC
TTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
GTGATCTTCA
GACCTGGACG
ATATATATGA
GGGACAATTG
GAGAAGTGAA
TTATATAAAT
ATAAAGTAGT
AAAAATTGAA
CCATTAGGAG
TAGCACCCAC
CAAGGCAAAG
AGAAGAGTGG
TGCAGAGAGA
AAAAAGAGCA
GTGGGAATAG
GAGCTTTGTT
CCTTGGGTTC
TTGGGAGCAG
CAGGAAGCAC
TATGGGCGCA
GCGTCAATGA
CGCTGACGGT
ACAGGCCAGA
CAATTATTGT
CTGGTATAGT
GCAGCAGCAG
AACAATTTGC
TGAGGGCTAT
TGAGGCGCAA
CAGCATCTGT
TGCAACTCAC
AGTCTGGGGC
ATCAAGCAGC
TCCAGGCAAG
AATCCTGGCT
GTGGAAAGAT
ACCTAAAGGA
TCAACAGCTC
CTGGGGATTT
GGGGTTGCTC
TGGAAAACTC
ATTTGCACCA
CTGCTGTGCC
TTGGAATGCT
AGTTGGAGTA
ATAAATCTCT
GGAACAGATT
TGGAATCACA
CGACCTGGAT
GGAGTGGGAC
AGAGAAATTA
ACAATTACAC
AAGCTTAATA
CACTCCTTAA
TTGAAGAATC
GCAAAACCAG
CAAGAAAAGA
ATGAACAAGA
ATTATTGGAA
TTAGATAAAT
GGGCAAGTTT
GTGGAATTGG
TTTAACATAA
CAAATTGGCT
GTGGTATATA
AAATTATTCA
TAATGATAGT
AGGAGGCTTG
GTAGGTTTAA
GAATAGTTTT
TGCTGTACTT
TCTATAGTGA
ATAGAGTTAG
GCAGGGATAT
TCACCATTAT
CGTTTCAGAC
CCACCTCCCA
ACCCCGAGGG
GACCCGACAG
GCCCGAAGGA
ATAGAAGAAG
AAGGTGGAGA
GAGAGACAGA
GACAGATCCA
TTCGATTAGT
GAACGGATCT
CGACGGTCG