产品货号：P64552
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV2-CMV-Eif3a(mouse)-3×FLAG-IRES-Puro |
|---|---|
| 别称: | NM_010123.3;A830012B05Rik/Csma/Eif3/Eif3s10/mKIAA0139 |
| 基因长: | 4035bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 小鼠 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | EF1a |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | BL21(DE3) |
| 温度: | 30度 |
| 背景载体: | pLV2-MCS-3×FLAG-IRES-Puro |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATAAGCTT
GGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCTATTTCCG
GTGAATTCGC
CACCATGCCG
GCCTATTTCC
AGAGGCCGGA
AAATGCCCTC
AAGCGCGCCA
ACGAATTTCT
TGAGGTTGGC
AAAAAGCAGC
CTGCCCTGGA
TGTTCTTTAT
GATGTAATGA
AAAGTAAGAA
GCACAGAACA
TGGCAGAAGA
TACATGAGCC
AATTATGCTG
AAATACTTGG
AACTGTGTGT
GGATCTTCGT
AAGAGCCACT
TGGCTAAGGA
AGGGTTATAT
CAATATAAGA
ACATATGCCA
ACAGGTAAAC
ATTAAATCTT
TAGAAGATGT
TGTTAGGGCA
TATTTGAAAT
TAGCAGAGGA
AAAAACAGAA
GCTGCTAAAG
AAGAGTCCCA
ACAAATGGTG
TTAGATATAG
AAGATCTGGA
TAATATTCAG
ACTCCTGAGA
GTGTTCTCTT
AAGTGCAGTA
AGTGGGGAAG
ATACTCAGGA
TCGTACTGAC
AGATTGCTAC
TGACTCCCTG
GGTCAAGTTC
CTGTGGGAAT
CATACAGGCA
GTGTTTGGAC
CTTCTTCGAA
ACAATTCTAG
AGTAGAGCGC
CTTTACCATG
ATATCGCCCA
ACAAGCTTTC
AAATTCTGCC
TCCAGTATAC
TCGGAAGGCT
GAGTTCCGCA
AGCTATGTGA
CAACTTGCGA
ATGCACTTAT
CCCAGATTCA
GCGCCACCAT
AACCAAAGCA
CAGCAATTAA
TCTTAATAAT
CCAGAGAGCC
AGTCTATGCA
TTTGGAAACC
AGACTTGTTC
AGTTGGACAG
TGCTATCAGC
ATGGAATTAT
GGCAGGAAGC
CTTCAAAGCT
GTGGAAGATA
TTCATGGACT
ATTTTCCTTG
TCTAAGAAAC
CACCTAAGCC
TCAGTTGATG
GCAAATTACT
ATAACAAAGT
TTCAACAGTG
TTTTGGAAAT
CTGGAAATGC
TTTGTTCCAT
GCATCTACAC
TTCATCGTCT
TTATCATCTG
TCTAGAGAAA
TGAGAAAGAA
TCTTACACAA
GAAGAGATGC
AAAGAATGTC
TACTAGAGTC
CTTTTGGCGA
CTCTTTCCAT
TCCTATTACT
CCTGAGCGAA
CTGATATTGC
TCGACTTTTG
GATATGGATG
GTATTATAGT
TGAGAAGCAG
CGACGCCTTG
CCACATTGCT
AGGTCTTCAA
GCCCCACCCA
CACGAATCGG
CCTAATTAAT
GATATGGTCA
GGTTCAGTGT
GCTACAGTAT
GTTGTCCCAG
AAGTGAAAGA
CCTTTACAAC
TGGTTGGAGG
TGGAATTCAA
CCCACTAAAA
CTCTGTGAGA
GAGTTACAAA
GGTATTAAAT
TGGGTTAGGG
AACAACCTGA
AAAAGAACCA
GAATTGCAAC
AATATGTACC
ACAACTCCAG
AACAATACCA
TACTCCGCCT
TCTGCAACAG
GTGGCACAGA
TTTATCAGAG
CATTGAGTTT
TCTCGTTTGA
CTTCTCTGGT
TCCTTTTGTT
GATGCTTTCC
AACTGGAACG
GGCCATAGTA
GATGCAGCCA
GGCACTGTGA
CCTGCAGGTA
CGTATTGACC
ATACTTCCCG
GACTCTGAGT
TTTGGATCAG
ATTTGAATTA
TGCAACTCGA
GAAGATGCCC
CAGTTGGCCC
TCATCTGCAG
AGCATGCCTT
CAGAGCAGAT
AAGAAACCAG
CTCACGGCCA
TGTCCTCAGT
GCTTGCCAAA
GCACTTGAAG
TCATCAGACC
TGCTCACATT
CTGCAAGAGA
AAGAAGAACA
GCATCAATTG
GCAGTTAATG
CATATCTTAA
AAATTCAAGA
AAAGAGCACC
AGAGGATCCT
GGCTCGGAGA
CAGACAATTG
AGGAAAGAAA
AGAGCGTCTT
GAGAGTCTGA
ATATTCAACG
TGAGAAGGAA
GAACTCGAGC
AGAGGGAAGC
TGAACTCCAG
AAAGTACGAA
AGGCTGAAGA
AGAAAGGCTG
CGCCAAGAGG
CAAAGGAGCG
AGAGAAGGAA
CGAATCCTTC
AAGAACACGA
GCAAATCAAG
AAGAAAACTG
TTCGGGAGCG
GTTAGAGCAG
ATCAAGAAGA
CAGAGCTGGG
CGCCAAAGCA
TTTAAAGATA
TTGACATTGA
AGACCTTGAA
GAACTGGATC
CAGATTTTAT
TATGGCCAAA
CAGGTTGAAC
AACTGGAGAA
AGAAAAGAAG
GAACTTCAGG
AACGCCTGAA
GAATCAAGAA
AAGAAGATTG
ACTATTTTGA
GAGAGCTAAG
CGTTTGGAAG
AAATTCCTTT
AATAAAGAGT
GCTTATGAGG
AACAAAGGGT
TAAAGACATG
GACCTGTGGG
AACAGCAAGA
AGAAGAAAGA
ATCACTACAA
TGCAGCTAGA
ACGAGAAAAA
GCTCTGGAGC
ATAAGAATAG
GATGTCACGA
ATGTTGGAAG
ACAGAGATCT
ATTTGTGATG
CGCCTCAAAG
CTGCCCGGCA
GTCTGTCTAC
GAGGAAAAAC
TGAAACAGTT
TGAAGAGCGC
TTAGCAGAAG
AAAGGCATAG
TCGCCTAGAA
GATCGGAAAA
GGCAGCGGAA
AGAAGAACGC
AAAATAACTT
ATTACAGAGA
AAAGGAAGAA
GAAGAGCAGA
GGAGGGCAGA
GGAGCAGATG
CTCAAAGAGC
GAGAAGAAAG
AGAACGTGCT
GAGAGAGCAA
AACGGGAGGA
AGAACTTAGA
GAGTATCAGG
AGCGAGTCAA
GAAACTAGAA
GAAGTAGAAA
GGAAAAAGCG
GCAAAGAGAG
CTGGAAATTG
AAGAAAGGGA
AAGGCGCAGA
GAGGAAGAAA
GGAGACTTGG
TGATGATCCA
CTTTCTAGGA
AGGACTCTCG
GTGGGGAGAT
AGAGATTCAG
AAGGCACCTG
GAGGAAAGGA
CCAGAAGCTG
ACTCTGAGTG
GAGAAGAGGC
CCACCAGAAA
AGGAGTGGAG
ACGAGAAACT
CGGGATGATG
AGAGGCCTCA
CAGGAGAGAT
GAGGATCGGC
TGAGGCGCTT
GGGGGGTGAC
GATGAAGAGA
GAGAGTCTTC
TCTTAGACCG
GATGATGATC
GTATCCCCAG
GCGTGGCCTG
GATGATGACA
GAGGGCCTAG
ACGTGGTCCC
GATGAAGATA
GATTTTCTCG
TAGAGGAACA
GATGATGACC
GTCCTTCCTG
GCGTAATGCA
GATGATGATA
GGCCTCCCAG
GCGAATTGGT
GATGATGACA
GGGGAAGCTG
GCGTCACACA
GATGACGACA
GACCACCCAG
ACGGGGACTG
GATGATGAGA
GAGGAAGCTG
GCGTACAGCA
GATGAGGACA
GAGGACCTAG
ACGAGGGATG
GATGATGACC
GGGGGCCAAG
ACGAGGAGGT
GCTGATGATG
AACGGTCATC
CTGGCGGAAT
GCTGATGATG
ATAGGGGTCC
CAGGAGAGGC
ATGGATGATG
ATAGGGGTCC
CAGGCGAGGG
CTGGATGATG
ACCGAGGACC
TTGGAGGAAT
GCTGCTGAAG
ATAGGATTTC
CAGGAGAGGT
GCAGATGATG
ACCGAGGGCC
TTGGAGAAAT
ATGGATGATG
ATAGGGTTCC
TAGAAGGGGT
GATGATGCAA
GACCTGGTCC
TTGGAGACCA
TTTGTCAAGC
CAGGTGGATG
GAGAGAGAAA
GAAAAGGCTA
GAGAAGAGAG
TTGGGGTCCA
CCTCGAGAAT
CAAGACCATC
AGAAGAACGT
GAATGGGATA
GAGACAAAGA
GAAGGACAGA
GATAATCAAG
ATCGAGAGGA
GAATGACAAA
GACCTTGAAC
GAGATAGGGA
CAGAGAGAGA
GATGGGGACC
GGGAGGATCG
ATTCAGAAGA
CCCAGGGATG
AAGGTGGCTG
GAGAAGAGGA
CCAGCAGAAG
AATCTTCAAG
CTGGAGAGAT
TCAAGTCGCC
GTGATGATAG
GGACAGGGAA
GACCGTCGAC
GGGATAGAGA
TGATCGTCGT
GATCTGAGAG
ACCTGAGAGA
CAGAAGGGAT
TTAAGAGATG
ATAGAGATCG
GAGAGGACCT
CCCCTCAGAT
CAGAGCGAGA
AGAAGCAAGC
TCTTGGAGAC
GCACTGATGA
CAGGAAAGAT
GACCGGACTG
AAGAGAGGGA
TCCACCTCGT
CGTGTTCCTC
CCCCAGCTCT
TTCAAGAGAT
CGAGAAAGAG
AGCGAGAACG
AGAAGGTGAG
AAAGAGAAAG
CATCCTGGAG
AGCTGAGAAA
GATAGGGAGT
CCCTTCGTCG
TACTAAGAAT
GAAACTGATG
AAGATGGATG
GACCACAGTA
CGACGTCTCG
AGGACTACAA
AGACCATGAC
GGTGATTATA
AAGATCATGA
CATCGACTAC
AAGGATGACG
ATGACAAGTA
GGATCCCGCC
CCTCTCCCTC
CCCCCCCCCT
AACGTTACTG
GCCGAAGCCG
CTTGGAATAA
GGCCGGTGTG
CGTTTGTCTA
TATGTTATTT
TCCACCATAT
TGCCGTCTTT
TGGCAATGTG
AGGGCCCGGA
AACCTGGCCC
TGTCTTCTTG
ACGAGCATTC
CTAGGGGTCT
TTCCCCTCTC
GCCAAAGGAA
TGCAAGGTCT
GTTGAATGTC
GTGAAGGAAG
CAGTTCCTCT
GGAAGCTTCT
TGAAGACAAA
CAACGTCTGT
AGCGACCCTT
TGCAGGCAGC
GGAACCCCCC
ACCTGGCGAC
AGGTGCCTCT
GCGGCCAAAA
GCCACGTGTA
TAAGATACAC
CTGCAAAGGC
GGCACAACCC
CAGTGCCACG
TTGTGAGTTG
GATAGTTGTG
GAAAGAGTCA
AATGGCTCTC
CTCAAGCGTA
TTCAACAAGG
GGCTGAAGGA
TGCCCAGAAG
GTACCCCATT
GTATGGGATC
TGATCTGGGG
CCTCGGTGCA
CATGCTTTAC
ATGTGTTTAG
TCGAGGTTAA
AAAAACGTCT
AGGCCCCCCG
AACCACGGGG
ACGTGGTTTT
CCTTTGAAAA
ACACGATGAT
AAGCTTGCCA
CAACCCACAA
GGAGACGACC
TTCCATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCCGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGACCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTAGACGCGT
CTGGAACAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAGCTGACGT
CCTTTCCATG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCCTGG
AATTAATTCT
GCAGTCGAGA
CCTAGAAAAA
CATGGAGCAA
TCACAAGTAG
CAATACAGCA
GCTACCAATG
CTGATTGTGC
CTGGCTAGAA
GCACAAGAGG
AGGAGGAGGT
GGGTTTTCCA
GTCACACCTC
AGGTACCTTT
AAGACCAATG
ACTTACAAGG
CAGCTGTAGA
TCTTAGCCAC
TTTTTAAAAG
AAAAGAGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ACAAGATATC
CTTGATCTGT
GGATCTACCA
CACACAAGGC
TACTTCCCTG
ATTAGCAGAA
CTACACACCA
GGGCCAGGGG
TCAGATATCC
ACTGACCTTT
GGATGGTGCT
ACAAGCTAGT
ACCAGTTGAG
CCAGATAAGG
TAGAAGAGGC
CAATAAAGGA
GAGAACACCA
GCTTGTTACA
CCCTGTGAGC
CTGCATGGGA
TGGATGACCC
GGAGAGAGAA
GTGTTAGAGT
GGAGGTTTGA
CAGCCGCCTA
GCATTTCATC
ACGTGGCCCG
AGAGCTGCAT
CCGGAGTACT
TCAAGAACTG
CTGATATCGA
GCTTGCTACA
AGGGACTTTC
CGCTGGGGAC
TTTCCAGGGA
GGCGTGGCCT
GGGCGGGACT
GGGGAGTGGC
GAGCCCTCAG
ATCCTGCATA
TAAGCAGCTG
CTTTTTGCCT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTAG
TAGTTCATGT
CATCTTATTA
TTCAGTATTT
ATAACTTGCA
AAGAAATGAA
TATCAGAGAG
TGAGAGGCCT
TGACATTGCT
AGCGTTTACC
GTCGACCTCT
AGCTAGAGCT
TGGCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGAACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CGACGGATCG
GGAGATCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGGA
TCAACTGGAT
AACTCAAGCT
AACCAAAATC
ATCCCAAACT
TCCCACCCCA
TACCCTATTA
CCACTGCCAA
TTACCTGTGG
TTTCATTTAC
TCTAAACCTG
TGATTCCTCT
GAATTATTTT
CATTTTAAAG
AAATTGTATT
TGTTAAATAT
GTACTACAAA
CTTAGTAGTT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGT
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