名称: | pEF1a-SALL2(human)-EGFP-FLAG-IRES-Puro |
---|---|
别称: | NM_005407.3;COLB/HSAL2/p150(Sal2)/Sal-2/ZNF795 |
基因长: | 3024bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pKD-Flag-IRES2-Puro |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATATGTCGC
GTGTAGTCTT
ATGCAATACT
CTTGTAGTCT
TGCAACATGG
TAACGATGAG
TTAGCAACAT
GCCTTACAAG
GAGAGAAAAA
GCACCGTGCA
TGCCGATTGG
TGGAAGTAAG
GTGGTACGAT
CGTGCCTTAT
TAGGAAGGCA
ACAGACGGGT
CTGACATGGA
TTGGACGAAC
CACTGAATTG
CCGCATTGCA
GAGATATTGT
ATTTAAGTGC
CTAGCTCGAT
ACAATAAACG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACC
TGAAAGCGAA
AGGGAAACCA
GAGCTCTCTC
GACGCAGGAC
TCGGCTTGCT
GAAGCGCGCA
CGGCAAGAGG
CGAGGGGCGG
CGACTGGTGA
GTACGCCAAA
AATTTTGACT
AGCGGAGGCT
AGAAGGAGAG
AGATGGGTGC
GAGAGCGTCA
GTATTAAGCG
GGGGAGAATT
AGATCGCGAT
GGGAAAAAAT
TCGGTTAAGG
CCAGGGGGAA
AGAAAAAATA
TAAATTAAAA
CATATAGTAT
GGGCAAGCAG
GGAGCTAGAA
CGATTCGCAG
TTAATCCTGG
CCTGTTAGAA
ACATCAGAAG
GCTGTAGACA
AATACTGGGA
CAGCTACAAC
CATCCCTTCA
GACAGGATCA
GAAGAACTTA
GATCATTATA
TAATACAGTA
GCAACCCTCT
ATTGTGTGCA
TCAAAGGATA
GAGATAAAAG
ACACCAAGGA
AGCTTTAGAC
AAGATAGAGG
AAGAGCAAAA
CAAAAGTAAG
ACCACCGCAC
AGCAAGCGGC
CGCTGATCTT
CAGACCTGGA
GGAGGAGATA
TGAGGGACAA
TTGGAGAAGT
GAATTATATA
AATATAAAGT
AGTAAAAATT
GAACCATTAG
GAGTAGCACC
CACCAAGGCA
AAGAGAAGAG
TGGTGCAGAG
AGAAAAAAGA
GCAGTGGGAA
TAGGAGCTTT
GTTCCTTGGG
TTCTTGGGAG
CAGCAGGAAG
CACTATGGGC
GCAGCGTCAA
TGACGCTGAC
GGTACAGGCC
AGACAATTAT
TGTCTGGTAT
AGTGCAGCAG
CAGAACAATT
TGCTGAGGGC
TATTGAGGCG
CAACAGCATC
TGTTGCAACT
CACAGTCTGG
GGCATCAAGC
AGCTCCAGGC
AAGAATCCTG
GCTGTGGAAA
GATACCTAAA
GGATCAACAG
CTCCTGGGGA
TTTGGGGTTG
CTCTGGAAAA
CTCATTTGCA
CCACTGCTGT
GCCTTGGAAT
GCTAGTTGGA
GTAATAAATC
TCTGGAACAG
ATTTGGAATC
ACACGACCTG
GATGGAGTGG
GACAGAGAAA
TTAACAATTA
CACAAGCTTA
ATACACTCCT
TAATTGAAGA
ATCGCAAAAC
CAGCAAGAAA
AGAATGAACA
AGAATTATTG
GAATTAGATA
AATGGGCAAG
TTTGTGGAAT
TGGTTTAACA
TAACAAATTG
GCTGTGGTAT
ATAAAATTAT
TCATAATGAT
AGTAGGAGGC
TTGGTAGGTT
TAAGAATAGT
TTTTGCTGTA
CTTTCTATAG
TGAATAGAGT
TAGGCAGGGA
TATTCACCAT
TATCGTTTCA
GACCCACCTC
CCAACCCCGA
GGGGACCCGA
CAGGCCCGAA
GGAATAGAAG
AAGAAGGTGG
AGAGAGAGAC
AGAGACAGAT
CCATTCGATT
AGTGAACGGA
TCTCGACGGT
ATCGATGTCG
ACGATAAGCT
TTGCAAAGAT
GGATAAAGTT
TTAAACAGAG
AGGAATCTTT
GCAGCTAATG
GACCTTCTAG
GTCTTGAAAG
GAGTGGGAAT
TGGCTCCGGT
GCCCGTCAGT
GGGCAGAGCG
CACATCGCCC
ACAGTCCCCG
AGAAGTTGGG
GGGAGGGGTC
GGCAATTGAA
CCGGTGCCTA
GAGAAGGTGG
CGCGGGGTAA
ACTGGGAAAG
TGATGTCGTG
TACTGGCTCC
GCCTTTTTCC
CGAGGGTGGG
GGAGAACCGT
ATATAAGTGC
AGTAGTCGCC
GTGAACGTTC
TTTTTCGCAA
CGGGTTTGCC
GCCAGAACAC
AGGTAAGTGC
CGTGTGTGGT
TCCCGCGGGC
CTGGCCTCTT
TACGGGTTAT
GGCCCTTGCG
TGCCTTGAAT
TACTTCCACT
GGCTGCAGTA
CGTGATTCTT
GATCCCGAGC
TTCGGGTTGG
AAGTGGGTGG
GAGAGTTCGA
GGCCTTGCGC
TTAAGGAGCC
CCTTCGCCTC
GTGCTTGAGT
TGAGGCCTGG
CCTGGGCGCT
GGGGCCGCCG
CGTGCGAATC
TGGTGGCACC
TTCGCGCCTG
TCTCGCTGCT
TTCGATAAGT
CTCTAGCCAT
TTAAAATTTT
TGATGACCTG
CTGCGACGCT
TTTTTTCTGG
CAAGATAGTC
TTGTAAATGC
GGGCCAAGAT
CTGCACACTG
GTATTTCGGT
TTTTGGGGCC
GCGGGCGGCG
ACGGGGCCCG
TGCGTCCCAG
CGCACATGTT
CGGCGAGGCG
GGGCCTGCGA
GCGCGGCCAC
CGAGAATCGG
ACGGGGGTAG
TCTCAAGCTG
GCCGGCCTGC
TCTGGTGCCT
GGCCTCGCGC
CGCCGTGTAT
CGCCCCGCCC
TGGGCGGCAA
GGCTGGCCCG
GTCGGCACCA
GTTGCGTGAG
CGGAAAGATG
GCCGCTTCCC
GGCCCTGCTG
CAGGGAGCTC
AAAATGGAGG
ACGCGGCGCT
CGGGAGAGCG
GGCGGGTGAG
TCACCCACAC
AAAGGAAAAG
GGCCTTTCCG
TCCTCAGCCG
TCGCTTCATG
TGACTCCACG
GAGTACCGGG
CGCCGTCCAG
GCACCTCGAT
TAGTTCTCGA
GCTTTTGGAG
TACGTCGTCT
TTAGGTTGGG
GGGAGGGGTT
TTATGCGATG
GAGTTTCCCC
ACACTGAGTG
GGTGGAGACT
GAAGTTAGGC
CAGCTTGGCA
CTTGATGTAA
TTCTCCTTGG
AATTTGCCCT
TTTTGAGTTT
GGATCTTGGT
TCATTCTCAA
GCCTCAGACA
GTGGTTCAAA
GTTTTTTTCT
TCCATTTCAG
GTGTCGTGAG
GAATTTCGAC
ATTTAAATTT
AATTAATCTC
GACGGTATCG
GTTAACTTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCCGATC
ACGAGACTAG
CCTCGAGGTT
TAAACTACGG
GATCCGCCAC
CATGTCTCGG
CGAAAGCAGC
GGAAACCCCA
ACAGTTAATC
TCGGACTGCG
AAGGTCCCAG
CGCGTCTGAG
AACGGTGATG
CTAGCGAGGA
GGATCACCCC
CAAGTCTGTG
CCAAGTGCTG
CGCACAATTC
ACTGACCCAA
CTGAATTCCT
CGCCCACCAG
AACGCATGTT
CTACTGACCC
TCCTGTAATG
GTGATAATTG
GGGGCCAGGA
GAACCCCAAC
AACTCTTCGG
CCTCCTCTGA
ACCCCGGCCT
GAGGGTCACA
ATAATCCTCA
GGTCATGGAC
ACAGAGCATA
GCAACCCCCC
AGATTCTGGG
TCCTCCGTGC
CCACGGATCC
CACCTGGGGC
CCAGAGAGGA
GAGGAGAGGA
GTCTTCAGGG
CATTTCCTGG
TCGCTGCCAC
AGGTACAGCG
GCTGGGGGAG
GCGGGGGCCT
GATCTTGGCC
AGTCCCAAGC
TGGGAGCAAC
CCCATTACCT
CCAGAATCGA
CCCCTGCACC
CCCTCCTCCT
CCACCACCCC
CTCCGCCCCC
AGGGGTAGGC
AGTGGCCACT
TGAATATCCC
CCTGATCTTG
GAAGAGCTAC
GGGTGCTGCA
GCAGCGGCAG
ATCCATCAGA
TGCAGATGAC
TGAGCAAATC
TGCAGGCAGG
TGCTGTTGCT
TGGCTCCTTA
GGCCAGACGG
TGGGTGCCCC
TGCCAGTCCC
TCAGAGCTAC
CTGGGACAGG
GACTGCCTCT
TCCACCAAGC
CCCTACTACC
CCTCTTCAGC
CCCATCAAGC
CTGTCCAAAC
CAGCAAGACA
CTGGCATCTT
CCTCCTCCTC
CTCCTCTTCC
TCTTCAGGGG
CAGAAACGCC
CAAGCAGGCC
TTCTTCCACC
TTTACCACCC
ACTGGGGTCA
CAGCATCCTT
TCTCTGCTGG
AGGGGTTGGG
CGAAGCCACA
AACCCACCCC
TGCCCCTTCC
CCAGCCTTGC
CAGGCAGCAC
AGATCAGCTG
ATTGCCTCGC
CTCATCTGGC
ATTCCCAAGC
ACCACGGGAC
TACTGGCAGC
ACAGTGTCTT
GGGGCAGCCC
GAGGCCTTGA
GGCCACTGCC
TCCCCAGGGC
TCCTGAAGCC
AAAGAATGGA
AGTGGTGAGC
TGAGCTACGG
AGAAGTGATG
GGTCCCTTGG
AGAAGCCTGG
TGGAAGGCAC
AAATGCCGCT
TCTGTGCCAA
AGTATTTGGC
AGTGACAGTG
CCCTGCAGAT
CCACCTTCGT
TCCCACACGG
GTGAGAGGCC
CTATAAGTGC
AATGTCTGTG
GAAACCGTTT
TACCACCCGT
GGCAACCTCA
AAGTGCATTT
CCACCGGCAT
CGTGAGAAGT
ACCCACATGT
GCAGATGAAC
CCACACCCAG
TACCAGAGCA
CCTAGACTAT
GTCATTACCA
GCAGTGGCTT
GCCTTATGGT
ATGTCCGTGC
CACCAGAGAA
GGCCGAGGAG
GAGGCAGCCA
CTCCAGGTGG
AGGGGTTGAG
CGCAAGCCTC
TGGTGGCCTC
CACAACAGCA
CTCAGTGCCA
CAGAGAGCCT
GACTCTGCTC
TCCACCAGTG
CAGGCACAGC
CACGGCTCCA
GGACTCCCTG
CTTTCAATAA
GTTTGTGCTC
ATGAAAGCAG
TGGAACCCAA
GAATAAAGCT
GATGAAAACA
CCCCCCCAGG
GAGTGAGGGC
TCAGCCATCA
GTGGAGTGGC
AGAAAGTAGC
ACGGCAACTC
GCATGCAACT
AAGTAAGTTG
GTGACTTCAC
TACCAAGCTG
GGCACTGCTT
ACCAACCACT
TCAAGTCCAC
TGGCAGCTTC
CCCTTCCCCT
ATGTGCTAGA
GCCCTTGGGG
GCCTCACCCT
CTGAGACATC
AAAGCTGCAG
CAACTGGTAG
AAAAGATTGA
CCGGCAAGGA
GCTGTGGCGG
TGACCTCAGC
TGCCTCAGGA
GCCCCCACCA
CCTCTGCCCC
TGCACCTTCA
TCCTCAGCCT
CTTCTGGACC
TAACCAGTGT
GTCATCTGTC
TCCGAGTGCT
TAGCTGTCCT
CGGGCCCTAC
GCCTTCATTA
TGGCCAACAT
GGAGGTGAGA
GGCCCTTCAA
ATGCAAAGTG
TGTGGCAGAG
CCTTCTCCAC
CAGGGGTAAT
CTGCGTGCAC
ATTTCGTGGG
CCACAAGGCC
AGTCCAGCTG
CCCGGGCACA
GAATTCCTGC
CCCATCTGCC
AGAAGAAGTT
CACCAATGCT
GTCACTCTGC
AGCAGCATGT
CCGGATGCAC
CTGGGGGGCC
AGATCCCCAA
CGGTGGTACT
GCACTCCCTG
AAGGTGGAGG
AGCTGCTCAG
GAGAATGGCT
CCGAGCAATC
TACAGTCTCC
GGGGCACGGA
GTTTCCCCCA
GCAGCAGTCC
CAGCAGCCAT
CACCGGAAGA
GGAGTTGTCT
GAGGAGGAGG
AAGAGGAGGA
TGAGGAAGAA
GAGGAAGATG
TGACTGATGA
AGATTCCCTG
GCAGGGAGAG
GCTCAGAGAG
TGGAGGTGAG
AAGGCAATAT
CAGTGAGAGG
TGATTCAGAA
GAGGCATCTG
GGGCAGAGGA
GGAGGTGGGG
ACAGTGGCGG
CAGCAGCCAC
AGCTGGGAAG
GAGATGGACA
GTAATGAGAA
AACTACTCAA
CAGTCTTCTT
TGCCACCACC
ACCACCACCT
GACAGCCTGG
ATCAGCCTCA
GCCAATGGAG
CAGGGAAGCA
GTGGTGTTTT
AGGAGGCAAG
GAAGAGGGGG
GCAAACCGGA
GAGAAGCTCA
AGTCCGGCAT
CAGCACTCAC
CCCAGAAGGG
GAAGCCACCA
GCGTGACCTT
GGTAGAGGAG
CTGAGCCTGC
AGGAGGCAAT
GAGAAAGGAG
CCAGGAGAGA
GCAGCAGCAG
AAAGGCCTGC
GAAGTGTGTG
GCCAGGCCTT
TCCCTCCCAG
GCAGCTCTGG
AGGAGCATCA
GAAGACCCAC
CCCAAGGAGG
GGCCGCTCTT
CACTTGTGTT
TTCTGCAGGC
AGGGCTTTCT
TGAGCGGGCT
ACCCTCAAGA
AGCATATGCT
CCTGGCACAC
CACCAGGTAC
AGCCCTTTGC
CCCCCATGGC
CCTCAGAATA
TTGCTGCTCT
TTCTCTAGTC
CCTGGCTGTT
CGCCTTCCAT
CACCTCCACA
GGGCTCTCCC
CCTTTCCCCG
AAAAGATGAC
CCCACGATCC
CACGCGTCCT
AGCGCTACCG
GTCGCCACCA
TGGTGAGCAA
GGGCGAGGAG
CTGTTCACCG
GGGTGGTGCC
CATCCTGGTC
GAGCTGGACG
GCGACGTAAA
CGGCCACAAG
TTCAGCGTGT
CCGGCGAGGG
CGAGGGCGAT
GCCACCTACG
GCAAGCTGAC
CCTGAAGTTC
ATCTGCACCA
CCGGCAAGCT
GCCCGTGCCC
TGGCCCACCC
TCGTGACCAC
CCTGACCTAC
GGCGTGCAGT
GCTTCAGCCG
CTACCCCGAC
CACATGAAGC
AGCACGACTT
CTTCAAGTCC
GCCATGCCCG
AAGGCTACGT
CCAGGAGCGC
ACCATCTTCT
TCAAGGACGA
CGGCAACTAC
AAGACCCGCG
CCGAGGTGAA
GTTCGAGGGC
GACACCCTGG
TGAACCGCAT
CGAGCTGAAG
GGCATCGACT
TCAAGGAGGA
CGGCAACATC
CTGGGGCACA
AGCTGGAGTA
CAACTACAAC
AGCCACAACG
TCTATATCAT
GGCCGACAAG
CAGAAGAACG
GCATCAAGGT
GAACTTCAAG
ATCCGCCACA
ACATCGAGGA
CGGCAGCGTG
CAGCTCGCCG
ACCACTACCA
GCAGAACACC
CCCATCGGCG
ACGGCCCCGT
GCTGCTGCCC
GACAACCACT
ACCTGAGCAC
CCAGTCCGCC
CTGAGCAAAG
ACCCCAACGA
GAAGCGCGAT
CACATGGTCC
TGCTGGAGTT
CGTGACCGCC
GCCGGGATCA
CTCTCGGCAT
GGACGAGCTG
TACAAGTCCG
GACTCAGATC
TCGATAGCTA
GTAGCTAGCT
AGCTAGTCGA
GCTCAACTTC
GAATTCGACT
ACAAGGACGA
CGATGACAAG
TGAGCCCCTC
TCCCTCCCCC
CCCCCTAACG
TTACTGGCCG
AAGCCGCTTG
GAATAAGGCC
GGTGTGCGTT
TGTCTATATG
TTATTTTCCA
CCATATTGCC
GTCTTTTGGC
AATGTGAGGG
CCCGGAAACC
TGGCCCTGTC
TTCTTGACGA
GCATTCCTAG
GGGTCTTTCC
CCTCTCGCCA
AAGGAATGCA
AGGTCTGTTG
AATGTCGTGA
AGGAAGCAGT
TCCTCTGGAA
GCTTCTTGAA
GACAAACAAC
GTCTGTAGCG
ACCCTTTGCA
GGCAGCGGAA
CCCCCCACCT
GGCGACAGGT
GCCTCTGCGG
CCAAAAGCCA
CGTGTATAAG
ATACACCTGC
AAAGGCGGCA
CAACCCCAGT
GCCACGTTGT
GAGTTGGATA
GTTGTGGAAA
GAGTCAAATG
GCTCTCCTCA
AGCGTATTCA
ACAAGGGGCT
GAAGGATGCC
CAGAAGGTAC
CCCATTGTAT
GGGATCTGAT
CTGGGGCCTC
GGTGCACATG
CTTTACATGT
GTTTAGTCGA
GGTTAAAAAA
ACGTCTAGGC
CCCCCGAACC
ACGGGGACGT
GGTTTTCCTT
TGAAAAACAC
GATGATAATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGCGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGAACT
AGTCATATGA
TAATCAACCT
CTGGATTACA
AAATTTGTGA
AAGATTGACT
GGTATTCTTA
ACTATGTTGC
TCCTTTTACG
CTATGTGGAT
ACGCTGCTTT
AATGCCTTTG
TATCATGCTA
TTGCTTCCCG
TATGGCTTTC
ATTTTCTCCT
CCTTGTATAA
ATCCTGGTTG
CTGTCTCTTT
ATGAGGAGTT
GTGGCCCGTT
GTCAGGCAAC
GTGGCGTGGT
GTGCACTGTG
TTTGCTGACG
CAACCCCCAC
TGGTTGGGGC
ATTGCCACCA
CCTGTCAGCT
CCTTTCCGGG
ACTTTCGCTT
TCCCCCTCCC
TATTGCCACG
GCGGAACTCA
TCGCCGCCTG
CCTTGCCCGC
TGCTGGACAG
GGGCTCGGCT
GTTGGGCACT
GACAATTCCG
TGGTGTTGTC
GGGGAAGCTG
ACGTCCTTTC
CATGGCTGCT
CGCCTGTGTT
GCCACCTGGA
TTCTGCGCGG
GACGTCCTTC
TGCTACGTCC
CTTCGGCCCT
CAATCCAGCG
GACCTTCCTT
CCCGCGGCCT
GCTGCCGGCT
CTGCGGCCTC
TTCCGCGTCT
TCGCCTTCGC
CCTCAGACGA
GTCGGATCTC
CCTTTGGGCC
GCCTCCCCGC
ATCGGTACGT
ATGGCCAGGT
ACCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GGGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATCTGCTTT
TTGCTTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGCATCTAG
AAATGAATAT
CAGAGAGTGA
GAGGAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGCTCT
AGCTATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGACTTTTG
CAGAGACGGC
CCAAATTCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGT