名称: | pEF1a-Pml(mouse)-tdTomato-Neo |
---|---|
别称: | NM_178087.4;1200009E24Rik/AI661194/Trim19 |
基因长: | 2658bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 红色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pEF1a-MCS-Neo |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGCGTTGACA
TTGATTATTG
ACTAGGCTTT
TGCAAAAAGC
TTTGCAAAGA
TGGATAAAGT
TTTAAACAGA
GAGGAATCTT
TGCAGCTAAT
GGACCTTCTA
GGTCTTGAAA
GGAGTGGGAA
TTGGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACCGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGGTAAGTG
CCGTGTGTGG
TTCCCGCGGG
CCTGGCCTCT
TTACGGGTTA
TGGCCCTTGC
GTGCCTTGAA
TTACTTCCAC
CTGGCTGCAG
TACGTGATTC
TTGATCCCGA
GCTTCGGGTT
GGAAGTGGGT
GGGAGAGTTC
GAGGCCTTGC
GCTTAAGGAG
CCCCTTCGCC
TCGTGCTTGA
GTTGAGGCCT
GGCCTGGGCG
CTGGGGCCGC
CGCGTGCGAA
TCTGGTGGCA
CCTTCGCGCC
TGTCTCGCTG
CTTTCGATAA
GTCTCTAGCC
ATTTAAAATT
TTTGATGACC
TGCTGCGACG
CTTTTTTTCT
GGCAAGATAG
TCTTGTAAAT
GCGGGCCAAG
ATCTGCACAC
TGGTATTTCG
GTTTTTGGGG
CCGCGGGCGG
CGACGGGGCC
CGTGCGTCCC
AGCGCACATG
TTCGGCGAGG
CGGGGCCTGC
GAGCGCGGCC
ACCGAGAATC
GGACGGGGGT
AGTCTCAAGC
TGGCCGGCCT
GCTCTGGTGC
CTGGCCTCGC
GCCGCCGTGT
ATCGCCCCGC
CCTGGGCGGC
AAGGCTGGCC
CGGTCGGCAC
CAGTTGCGTG
AGCGGAAAGA
TGGCCGCTTC
CCGGCCCTGC
TGCAGGGAGC
TCAAAATGGA
GGACGCGGCG
CTCGGGAGAG
CGGGCGGGTG
AGTCACCCAC
ACAAAGGAAA
AGGGCCTTTC
CGTCCTCAGC
CGTCGCTTCA
TGTGACTCCA
CGGAGTACCG
GGCGCCGTCC
AGGCACCTCG
ATTAGTTCTC
GAGCTTTTGG
AGTACGTCGT
CTTTAGGTTG
GGGGGAGGGG
TTTTATGCGA
TGGAGTTTCC
CCACACTGAG
TGGGTGGAGA
CTGAAGTTAG
GCCAGCTTGG
CACTTGATGT
AATTCTCCTT
GGAATTTGCC
CTTTTTGAGT
TTGGATCTTG
GTTCATTCTC
AAGCCTCAGA
CAGTGGTTCA
AAGTTTTTTT
CTTCCATTTC
AGGTGTCGTG
AGGAATTAGC
TTGGTACGCT
AGCGTTTAAA
CTTAAGCTTG
GTACCGCCAC
CATGGAAACT
GAACCAGTTT
CCGTGCAGAA
GGTACCTGCA
CCCCCTGGAT
CTCCCTGTCG
ACAACAGGAC
TCTGCCCTGA
CCCCCACGCC
CACCATGCCT
CCCCCAGAGG
AACCCTCCGA
AGACTATGAA
CACAGCCAAA
GCCCTGCAGA
GCAAGCCATA
CAGGAGGAAT
TTCAGTTTCT
GCGCTGCCCG
AGCTGCCAGG
CCCAAGCCAA
GTGCCCCAAA
CTGCTGCCTT
GCCTGCACAC
GCTGTGCTCC
GGATGCCTGG
AGGCGCCTGG
CCTGCAGTGC
CCCATCTGCA
AGGCTCCTGG
GCAGGCCGAT
GCTAATGGGG
AGGCCCTGGA
TAACGTGTTC
TTCGAGAGCC
TGCAGCGGCG
CCTGGCGGTG
TTCCGGCAGA
TCGTGGATGC
TCAGGCTGCG
TGCACCCGCT
GCAAAGGCTT
GGCCGACTTC
TGGTGTTTCG
AGTGTGAACA
GCTCATTTGC
AGCAAGTGCT
TCGAAGCACA
CCAGTGGTAC
CTCAAGCATG
AGGCCCGGCC
CCTGGCTGAT
CTCCGCGACA
ATTCAGTGAG
CAGCTTCCTC
GACAGTACGC
GCAAGTCCAA
TATCTTCTGC
TCCAATACCA
ACCACCGCAA
CCCTGCGCTG
ACTGACATCT
ACTGCCGAGG
CTGCGCCAAG
CCTCTGTGTT
GCACATGCGC
GCTCCTGGAC
CGCAACCACA
GCCATCTCCA
TTGCGATATT
GGTGAGGAGA
TTCAGCAGTG
GCATGAGGAA
CTAGGCACCA
TGACACAGAC
TCTGGAGGAG
CAGGGCAGAA
CCTTCGACAG
TGCCCATGCA
CAGATGTGCT
CAGCTATAGG
ACAGCTGGAC
CACGCACGCG
CAGACATTGA
GAAGCAGATC
CGCGCACGCG
TGCGCCAGGT
GGTAGACTAC
GTGCAGGCTC
AGGAGCGCGA
GCTGCTCGAG
GCGGTGAATG
ACCGCTACCA
GCGCGACTAC
CAGGAAATAG
CTGGCCAGCT
GAGCTGCCTG
GAAGCTGTGC
TGCAGCGCAT
CCGCACTAGT
GGGGCGCTGG
TCAAGAGGAT
GAAGCTCTAT
GCCTCCGACC
AGGAGGTGCT
GGATATGCAC
AGCTTTCTGC
GCAAGGCACT
CTGTAGCTTG
CGCCAGGAGG
AGCCCCAGAA
CCAGAAAGTC
CAGCTGCTCA
CCAGAGGTTT
CGAGGAGTTC
AAGCTGTGCC
TGCAGGACTT
CATCTCCTGC
ATCACCCAGA
GGATAAATGC
AGCTGTAGCC
AGCCCAGAGG
CAGCCAGCAA
TCAACCAGAG
GCAGCCAGCA
CTCACCCAGT
GACAACCAGC
ACGCCTGAGG
ACCTTGAGCA
GCCCAAAGAA
GTGCAGAGTG
TACAGGCTCA
GGCCCTAGAG
CTGTCTAAGA
CCCAACCTGT
GGCTATGGTA
AAAACAGTGC
CTGGAGCACA
CCCTGTACCA
GTGTATGCCT
TTTCAATGCA
AGGCCCCACC
TACAGAGAGG
AGGCTTCTCA
GACAGTCGGC
TCCATGAAGA
GGAAGTGCTC
CCACGAAGAT
TGCTCCAGGA
AGATCATCAA
GATGGAGTCC
ACAGAGGAGA
ACGAGGACAG
GTTGGCCACA
AGCTCCCCCG
AGCAGTCCTG
GCCCAGCACT
TTCAAGGCCA
CCTCCCCTCC
CCATCTGGAT
GGGACTTCCA
ACCCTGAGAG
CACCGTCCCT
GAAAAAAAGA
TCCTCCTGCC
CAACAACAAT
CATGTTACCA
GTGACACAGG
GGAAACAGAG
GAGCGAGTTG
TGGTGATCAG
CAGCTCAGAG
GATTCGGACA
CCGAGAATCT
GTCCTCCCAC
GAGTTGGACG
ATAGCAGCAG
TGAGTCCAGC
AGCCTGCAGC
TGGAGGGCCC
CAATTCCCTC
AAGGCCTTGG
ATGAGAGCCT
CGCCGAACCC
CACTTAGAAG
ACAGGACCCT
GGTTTTCTTT
GACCTCAAGA
TTGACAATGA
AACCCAGAAA
ATTAGCCAGC
TGGCCGCGGT
GAACCGGGAA
AGCAAGTTCC
GTGTGCTCAT
CCAGCCCGAG
GCCTTCAGTG
TCTACTCCAA
AGCTGTCTCC
CTGGAGGCGG
GGCTCCGGCA
CTTCCTCAGC
TTCCTCACCA
CCATGCACCG
TCCCATCTTG
GCGTGTTCTA
GGCTGTGGGG
GCCGGGACTC
CCCATCTTCT
TCCAGACCCT
GAGTGATATT
AACAAGCTGT
GGGAATTCCA
GGACACCATC
TCAGGTTTCT
TGGCCGTGCT
GCCCCTCATC
CGGGAACGCA
TACCCGGCGC
TAGCAGCTTC
AAACTTGGGA
ACCTAGCCAA
GACCTACCTG
GCGAGAAACA
TGAGCGAACG
CAGCGCTCTG
GCTTCTGTGC
TGGCCATGAG
GGACTTGTGC
TGCCTCCTTG
AGATCTCCCC
AGGACTGCCG
CTGGCCCAGC
ATATCTACTC
CTTTAGTAGC
TTGCAGTGTT
TTGCTTCCCT
GCAGCCCCTA
ATTCAGGCCA
GCGTCCTGCC
ACAGTCCGAG
GCCCGCCTCT
TGGCCCTCCA
CAATGTGAGC
TTTGTAGAGT
TACTGAATGC
ATATCGCACC
AACAGGCAAG
AAGGCTTGAA
GAAGTATGTC
CACTATCTGA
GCCTGCAGAC
CACCCCGTTG
TCATCGTCGG
CTTCCACCCA
AGTTGCCCAA
TTCCTGCAGG
CTCTTAGCAC
CCACATGGAA
GGACTGTTGG
AAGGCCATGC
CCCTGCTGGG
GCAGAAGGCA
AAGCTGAAAG
TAAGGGATGC
CTGGCCGGTG
GAGGCGGATC
AGGAGGCGGT
GGATCCATGG
TGAGCAAGGG
CGAGGAGGTC
ATCAAAGAGT
TCATGCGCTT
CAAGGTGCGC
ATGGAGGGCT
CCATGAACGG
CCACGAGTTC
GAGATCGAGG
GCGAGGGCGA
GGGCCGCCCC
TACGAGGGCA
CCCAGACCGC
CAAGCTGAAG
GTGACCAAGG
GCGGCCCCCT
GCCCTTCGCC
TGGGACATCC
TGTCCCCCCA
GTTCATGTAC
GGCTCCAAGG
CGTACGTGAA
GCACCCCGCC
GACATCCCCG
ATTACAAGAA
GCTGTCCTTC
CCCGAGGGCT
TCAAGTGGGA
GCGCGTGATG
AACTTCGAGG
ACGGCGGTCT
GGTGACCGTG
ACCCAGGACT
CCTCCCTGCA
GGACGGCACG
CTGATCTACA
AGGTGAAGAT
GCGCGGCACC
AACTTCCCCC
CCGACGGCCC
CGTAATGCAG
AAGAAGACCA
TGGGCTGGGA
GGCCTCCACC
GAGCGCCTGT
ACCCCCGCGA
CGGCGTGCTG
AAGGGCGAGA
TCCACCAGGC
CCTGAAGCTG
AAGGACGGCG
GCCACTACCT
GGTGGAGTTC
AAGACCATCT
ACATGGCCAA
GAAGCCCGTG
CAACTGCCCG
GCTACTACTA
CGTGGACACC
AAGCTGGACA
TCACCTCCCA
CAACGAGGAC
TACACCATCG
TGGAACAGTA
CGAGCGCTCC
GAGGGCCGCC
ACCACCTGTT
CCTGGGGCAT
GGCACCGGCA
GCACCGGCAG
CGGCAGCTCC
GGCACCGCCT
CCTCCGAGGA
CAACAACATG
GCCGTCATCA
AAGAGTTCAT
GCGCTTCAAG
GTGCGCATGG
AGGGCTCCAT
GAACGGCCAC
GAGTTCGAGA
TCGAGGGCGA
GGGCGAGGGC
CGCCCCTACG
AGGGCACCCA
GACCGCCAAG
CTGAAGGTGA
CCAAGGGCGG
CCCCCTGCCC
TTCGCCTGGG
ACATCCTGTC
CCCCCAGTTC
ATGTACGGCT
CCAAGGCGTA
CGTGAAGCAC
CCCGCCGACA
TCCCCGATTA
CAAGAAGCTG
TCCTTCCCCG
AGGGCTTCAA
GTGGGAGCGC
GTGATGAACT
TCGAGGACGG
CGGTCTGGTG
ACCGTGACCC
AGGACTCCTC
CCTGCAGGAC
GGCACGCTGA
TCTACAAGGT
GAAGATGCGC
GGCACCAACT
TCCCCCCCGA
CGGCCCCGTA
ATGCAGAAGA
AGACCATGGG
CTGGGAGGCC
TCCACCGAGC
GCCTGTACCC
CCGCGACGGC
GTGCTGAAGG
GCGAGATCCA
CCAGGCCCTG
AAGCTGAAGG
ACGGCGGCCA
CTACCTGGTG
GAGTTCAAGA
CCATCTACAT
GGCCAAGAAG
CCCGTGCAAC
TGCCCGGCTA
CTACTACGTG
GACACCAAGC
TGGACATCAC
CTCCCACAAC
GAGGACTACA
CCATCGTGGA
ACAGTACGAG
CGCTCCGAGG
GCCGCCACCA
CCTGTTCCTG
TACGGCATGG
ACGAGCTGTA
CAAGTAGGGA
TCCTCAGACG
CGGCCGCGAC
TCTAGATCAT
AATCAGCCAT
ACCACATTTG
TAGAGGTTTT
ACTTGCTTTA
AAAAACCTCC
CACACCTCCC
CCTGAACCTG
AAACATAAAA
TGAATGCAAT
TGTTGTTGTT
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TAAGGCGTAA
ATTGTAAGCG
TTAATATTTT
GTTAAAATTC
GCGTTAAATT
TTTGTTAAAT
CAGCTCATTT
TTTAACCAAT
AGGCCGAAAT
CGGCAAAATC
CCTTATAAAT
CAAAAGAATA
GACCGAGATA
GGGTTGAGTG
TTGTTCCAGT
TTGGAACAAG
AGTCCACTAT
TAAAGAACGT
GGACTCCAAC
GTCAAAGGGC
GAAAAACCGT
CTATCAGGGC
GATGGCCCAC
TACGTGAACC
ATCACCCTAA
TCAAGTTTTT
TGGGGTCGAG
GTGCCGTAAA
GCACTAAATC
GGAACCCTAA
AGGGAGCCCC
CGATTTAGAG
CTTGACGGGG
AAAGCCGGCG
AACGTGGCGA
GAAAGGAAGG
GAAGAAAGCG
AAAGGAGCGG
GCGCTAGGGC
GCTGGCAAGT
GTAGCGGTCA
CGCTGCGCGT
AACCACCACA
CCCGCCGCGC
TTAATGCGCC
GCTACAGGGC
GCGTCAGGTG
GCACTTTTCG
GGGAAATGTG
CGCGGAACCC
CTATTTGTTT
ATTTTTCTAA
ATACATTCAA
ATATGTATCC
GCTCATGAGA
CAATAACCCT
GATAAATGCT
TCAATAATAT
TGAAAAAGGA
AGAGTCCTGA
GGCGGAAAGA
ACCAGCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAGATCGA
TCAAGAGACA
GGATGAGGAT
CGTTTCGCAT
GATTGAACAA
GATGGATTGC
ACGCAGGTTC
TCCGGCCGCT
TGGGTGGAGA
GGCTATTCGG
CTATGACTGG
GCACAACAGA
CAATCGGCTG
CTCTGATGCC
GCCGTGTTCC
GGCTGTCAGC
GCAGGGGCGC
CCGGTTCTTT
TTGTCAAGAC
CGACCTGTCC
GGTGCCCTGA
ATGAACTGCA
AGACGAGGCA
GCGCGGCTAT
CGTGGCTGGC
CACGACGGGC
GTTCCTTGCG
CAGCTGTGCT
CGACGTTGTC
ACTGAAGCGG
GAAGGGACTG
GCTGCTATTG
GGCGAAGTGC
CGGGGCAGGA
TCTCCTGTCA
TCTCACCTTG
CTCCTGCCGA
GAAAGTATCC
ATCATGGCTG
ATGCAATGCG
GCGGCTGCAT
ACGCTTGATC
CGGCTACCTG
CCCATTCGAC
CACCAAGCGA
AACATCGCAT
CGAGCGAGCA
CGTACTCGGA
TGGAAGCCGG
TCTTGTCGAT
CAGGATGATC
TGGACGAAGA
GCATCAGGGG
CTCGCGCCAG
CCGAACTGTT
CGCCAGGCTC
AAGGCGAGCA
TGCCCGACGG
CGAGGATCTC
GTCGTGACCC
ATGGCGATGC
CTGCTTGCCG
AATATCATGG
TGGAAAATGG
CCGCTTTTCT
GGATTCATCG
ACTGTGGCCG
GCTGGGTGTG
GCGGACCGCT
ATCAGGACAT
AGCGTTGGCT
ACCCGTGATA
TTGCTGAAGA
GCTTGGCGGC
GAATGGGCTG
ACCGCTTCCT
CGTGCTTTAC
GGTATCGCCG
CTCCCGATTC
GCAGCGCATC
GCCTTCTATC
GCCTTCTTGA
CGAGTTCTTC
TGAGCGGGAC
TCTGGGGTTC
GAAATGACCG
ACCAAGCGAC
GCCCAACCTG
CCATCACGAG
ATTTCGATTC
CACCGCCGCC
TTCTATGAAA
GGTTGGGCTT
CGGAATCGTT
TTCCGGGACG
CCGGCTGGAT
GATCCTCCAG
CGCGGGGATC
TCATGCTGGA
GTTCTTCGCC
CACCCTAGGG
GGAGGCTAAC
TGAAACACGG
AAGGAGACAA
TACCGGAAGG
AACCCGCGCT
ATGACGGCAA
TAAAAAGACA
GAATAAAACG
CACGGTGTTG
GGTCGTTTGT
TCATAAACGC
GGGGTTCGGT
CCCAGGGCTG
GCACTCTGTC
GATACCCCAC
CGAGACCCCA
TTGGGGCCAA
TACGCCCGCG
TTTCTTCCTT
TTCCCCACCC
CACCCCCCAA
GTTCGGGTGA
AGGCCCAGGG
CTCGCAGCCA
ACGTCGGGGC
GGCAGGCCCT
GCCATAGCCT
CAGGTTACTC
ATATATACTT
TAGATTGATT
TAAAACTTCA
TTTTTAATTT
AAAAGGATCT
AGGTGAAGAT
CCTTTTTGAT
AATCTCATGA
CCAAAATCCC
TTAACGTGAG
TTTTCGTTCC
ACTGAGCGTC
AGACCCCGTA
GAAAAGATCA
AAGGATCTTC
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTTCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT