名称: | pCMV-ITGA2(human)-3×FLAG-Neo |
---|---|
别称: | NM_002203.4;BR; CD49B; GPIa; HPA-5; VLA-2; VLAA2 |
基因长: | 3543bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pCMV-T7-MCS-3×FLAG-Neo |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGAGCTCGG
ATCCACTAGT
CCAGTGTGGT
GGAATTCGCC
ACCATGGGGC
CAGAACGGAC
AGGGGCCGCG
CCGCTGCCGC
TGCTGCTGGT
GTTAGCGCTC
AGTCAAGGCA
TTTTAAATTG
TTGTTTGGCC
TACAATGTTG
GTCTCCCAGA
AGCAAAAATA
TTTTCCGGTC
CTTCAAGTGA
ACAGTTTGGC
TATGCAGTGC
AGCAGTTTAT
AAATCCAAAA
GGCAACTGGT
TACTGGTTGG
TTCACCCTGG
AGTGGCTTTC
CTGAGAACCG
AATGGGAGAT
GTGTATAAAT
GTCCTGTTGA
CCTATCCACT
GCCACATGTG
AAAAACTAAA
TTTGCAAACT
TCAACAAGCA
TTCCAAATGT
TACTGAGATG
AAAACCAACA
TGAGCCTCGG
CTTGATCCTC
ACCAGGAACA
TGGGAACTGG
AGGTTTTCTC
ACATGTGGTC
CTCTGTGGGC
ACAGCAATGT
GGGAATCAGT
ATTACACAAC
GGGTGTGTGT
TCTGACATCA
GTCCTGATTT
TCAGCTCTCA
GCCAGCTTCT
CACCTGCAAC
TCAGCCCTGC
CCTTCCCTCA
TAGATGTTGT
GGTTGTGTGT
GATGAATCAA
ATAGTATTTA
TCCTTGGGAT
GCAGTAAAGA
ATTTTTTGGA
AAAATTTGTA
CAAGGCCTGG
ATATAGGCCC
CACAAAGACA
CAGGTGGGGT
TAATTCAGTA
TGCCAATAAT
CCAAGAGTTG
TGTTTAACTT
GAACACATAT
AAAACCAAAG
AAGAAATGAT
TGTAGCAACA
TCCCAGACAT
CCCAATATGG
TGGGGACCTC
ACAAACACAT
TCGGAGCAAT
TCAATATGCA
AGAAAATATG
CTTATTCAGC
AGCTTCTGGT
GGGCGACGAA
GTGCTACGAA
AGTAATGGTA
GTTGTAACTG
ACGGTGAATC
ACATGATGGT
TCAATGTTGA
AAGCTGTGAT
TGATCAATGC
AACCATGACA
ATATACTGAG
GTTTGGCATA
GCAGTTCTTG
GGTACTTAAA
CAGAAACGCC
CTTGATACTA
AAAATTTAAT
AAAAGAAATA
AAAGCAATCG
CTAGTATTCC
AACAGAAAGA
TACTTTTTCA
ATGTGTCTGA
TGAAGCAGCT
CTACTAGAAA
AGGCTGGGAC
ATTAGGAGAA
CAAATTTTCA
GCATTGAAGG
TACTGTTCAA
GGAGGAGACA
ACTTTCAGAT
GGAAATGTCA
CAAGTGGGAT
TCAGTGCAGA
TTACTCTTCT
CAAAATGATA
TTCTGATGCT
GGGTGCAGTG
GGAGCTTTTG
GCTGGAGTGG
GACCATTGTC
CAGAAGACAT
CTCATGGCCA
TTTGATCTTT
CCTAAACAAG
CCTTTGACCA
AATTCTGCAG
GACAGAAATC
ACAGTTCATA
TTTAGGTTAC
TCTGTGGCTG
CAATTTCTAC
TGGAGAAAGC
ACTCACTTTG
TTGCTGGTGC
TCCTCGGGCA
AATTATACCG
GCCAGATAGT
GCTATATAGT
GTGAATGAGA
ATGGCAATAT
CACGGTTATT
CAGGCTCACC
GAGGTGACCA
GATTGGCTCC
TATTTTGGTA
GTGTGCTGTG
TTCAGTTGAT
GTGGATAAAG
ACACCATTAC
AGACGTGCTC
TTGGTAGGTG
CACCAATGTA
CATGAGTGAC
CTAAAGAAAG
AGGAAGGAAG
AGTCTACCTG
TTTACTATCA
AAGAGGGCAT
TTTGGGTCAG
CACCAATTTC
TTGAAGGCCC
CGAGGGCATT
GAAAACACTC
GATTTGGTTC
AGCAATTGCA
GCTCTTTCAG
ACATCAACAT
GGATGGCTTT
AATGATGTGA
TTGTTGGTTC
ACCACTAGAA
AATCAGAATT
CTGGAGCTGT
ATACATTTAC
AATGGTCATC
AGGGCACTAT
CCGCACAAAG
TATTCCCAGA
AAATCTTGGG
ATCCGATGGA
GCCTTTAGGA
GCCATCTCCA
GTACTTTGGG
AGGTCCTTGG
ATGGCTATGG
AGATTTAAAT
GGGGATTCCA
TCACCGATGT
GTCTATTGGT
GCCTTTGGAC
AAGTGGTTCA
ACTCTGGTCA
CAAAGTATTG
CTGATGTAGC
TATAGAAGCT
TCATTCACAC
CAGAAAAAAT
CACTTTGGTC
AACAAGAATG
CTCAGATAAT
TCTCAAACTC
TGCTTCAGTG
CAAAGTTCAG
ACCTACTAAG
CAAAACAATC
AAGTGGCCAT
TGTATATAAC
ATCACACTTG
ATGCAGATGG
ATTTTCATCC
AGAGTAACCT
CCAGGGGGTT
ATTTAAAGAA
AACAATGAAA
GGTGCCTGCA
GAAGAATATG
GTAGTAAATC
AAGCACAGAG
TTGCCCCGAG
CACATCATTT
ATATACAGGA
GCCCTCTGAT
GTTGTCAACT
CTTTGGATTT
GCGTGTGGAC
ATCAGTCTGG
AAAACCCTGG
CACTAGCCCT
GCCCTTGAAG
CCTATTCTGA
GACTGCCAAG
GTCTTCAGTA
TTCCTTTCCA
CAAAGACTGT
GGTGAGGACG
GACTTTGCAT
TTCTGATCTA
GTCCTAGATG
TCCGACAAAT
ACCAGCTGCT
CAAGAACAAC
CCTTTATTGT
CAGCAACCAA
AACAAAAGGT
TAACATTTTC
AGTAACGCTG
AAAAATAAAA
GGGAAAGTGC
ATACAACACT
GGAATTGTTG
TTGATTTTTC
AGAAAACTTG
TTTTTTGCAT
CATTCTCCCT
GCCGGTTGAT
GGGACAGAAG
TAACATGCCA
GGTGGCTGCA
TCTCAGAAGT
CTGTTGCCTG
CGATGTAGGC
TACCCTGCTT
TAAAGAGAGA
ACAACAGGTG
ACTTTTACTA
TTAACTTTGA
CTTCAATCTT
CAAAACCTTC
AGAATCAGGC
GTCTCTCAGT
TTCCAAGCCT
TAAGTGAAAG
CCAAGAAGAA
AACAAGGCTG
ATAATTTGGT
CAACCTCAAA
ATTCCTCTCC
TGTATGATGC
TGAAATTCAC
TTAACAAGAT
CTACCAACAT
AAATTTTTAT
GAAATCTCTT
CGGATGGGAA
TGTTCCTTCA
ATCGTGCACA
GTTTTGAAGA
TGTTGGTCCA
AAATTCATCT
TCTCCCTGAA
GGTAACAACA
GGAAGTGTTC
CAGTAAGCAT
GGCAACTGTA
ATCATCCACA
TCCCTCAGTA
TACCAAAGAA
AAGAACCCAC
TGATGTACCT
AACTGGGGTG
CAAACAGACA
AGGCTGGTGA
CATCAGTTGT
AATGCAGATA
TCAATCCACT
GAAAATAGGA
CAAACATCTT
CTTCTGTATC
TTTCAAAAGT
GAAAATTTCA
GGCACACCAA
AGAATTGAAC
TGCAGAACTG
CTTCCTGTAG
TAATGTTACC
TGCTGGTTGA
AAGACGTTCA
CATGAAAGGA
GAATACTTTG
TTAATGTGAC
TACCAGAATT
TGGAACGGGA
CTTTCGCATC
ATCAACGTTC
CAGACAGTAC
AGCTAACGGC
AGCTGCAGAA
ATCAACACCT
ATAACCCTGA
GATATATGTG
ATTGAAGATA
ACACTGTTAC
GATTCCCCTG
ATGATAATGA
AACCTGATGA
GAAAGCCGAA
GTACCAACAG
GAGTTATAAT
AGGAAGTATA
ATTGCTGGAA
TCCTTTTGCT
GTTAGCTCTG
GTTGCAATTT
TATGGAAGCT
CGGCTTCTTC
AAAAGAAAAT
ATGAAAAGAT
GACCAAAAAT
CCAGATGAGA
TTGATGAGAC
CACAGAGCTC
AGTAGCCTCG
AGGACTACAA
AGACCATGAC
GGTGATTATA
AAGATCATGA
CATCGACTAC
AAGGATGACG
ATGACAAGTA
GTGAGGGCCC
GTTTAAACCC
GCTGATCAGC
CTCGACTGTG
CCTTCTAGTT
GCCAGCCATC
TGTTGTTTGC
CCCTCCCCCG
TGCCTTCCTT
GACCCTGGAA
GGTGCCACTC
CCACTGTCCT
TTCCTAATAA
AATGAGGAAA
TTGCATCGCA
TTGTCTGAGT
AGGTGTCATT
CTATTCTGGG
GGGTGGGGTG
GGGCAGGACA
GCAAGGGGGA
GGATTGGGAA
GACAATAGCA
GGCATGCTGG
GGATGCGGTG
GGCTCTATGG
CTTCTGAGGC
GGAAAGAACC
AGCTGGGGCT
CTAGGGGGTA
TCCCCACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCCTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TAGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGTCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGCCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTAATTCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCTGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAAAG
CTCCCGGGAG
CTTGTATATC
CATTTTCGGA
TCTGATCAAG
AGACAGGATG
AGGATCGTTT
CGCATGATTG
AACAAGATGG
ATTGCACGCA
GGTTCTCCGG
CCGCTTGGGT
GGAGAGGCTA
TTCGGCTATG
ACTGGGCACA
ACAGACAATC
GGCTGCTCTG
ATGCCGCCGT
GTTCCGGCTG
TCAGCGCAGG
GGCGCCCGGT
TCTTTTTGTC
AAGACCGACC
TGTCCGGTGC
CCTGAATGAA
CTGCAGGACG
AGGCAGCGCG
GCTATCGTGG
CTGGCCACGA
CGGGCGTTCC
TTGCGCAGCT
GTGCTCGACG
TTGTCACTGA
AGCGGGAAGG
GACTGGCTGC
TATTGGGCGA
AGTGCCGGGG
CAGGATCTCC
TGTCATCTCA
CCTTGCTCCT
GCCGAGAAAG
TATCCATCAT
GGCTGATGCA
ATGCGGCGGC
TGCATACGCT
TGATCCGGCT
ACCTGCCCAT
TCGACCACCA
AGCGAAACAT
CGCATCGAGC
GAGCACGTAC
TCGGATGGAA
GCCGGTCTTG
TCGATCAGGA
TGATCTGGAC
GAAGAGCATC
AGGGGCTCGC
GCCAGCCGAA
CTGTTCGCCA
GGCTCAAGGC
GCGCATGCCC
GACGGCGAGG
ATCTCGTCGT
GACCCATGGC
GATGCCTGCT
TGCCGAATAT
CATGGTGGAA
AATGGCCGCT
TTTCTGGATT
CATCGACTGT
GGCCGGCTGG
GTGTGGCGGA
CCGCTATCAG
GACATAGCGT
TGGCTACCCG
TGATATTGCT
GAAGAGCTTG
GCGGCGAATG
GGCTGACCGC
TTCCTCGTGC
TTTACGGTAT
CGCCGCTCCC
GATTCGCAGC
GCATCGCCTT
CTATCGCCTT
CTTGACGAGT
TCTTCTGAGC
GGGACTCTGG
GGTTCGAAAT
GACCGACCAA
GCGACGCCCA
ACCTGCCATC
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTC