名称: | pLV3-U6-PYCARD(human)-sgRNA1-Cas9-Neo |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-U6-MCS-sgRNA-Cas9-EGFP-Neo |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTAGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
ACCGGGCTGC
GCTTATCGCG
AGGGTTTTAG
AGCTAGAAAT
AGCAAGTTAA
AATAAGGCTA
GTCCGTTATC
AACTTGAAAA
AGTGGCACCG
AGTCGGTGCT
TTTTTGAATT
CGCTAGCTAG
GTCTTGAAAG
GAGTGGGAAT
TGGCTCCGGT
GCCCGTCAGT
GGGCAGAGCG
CACATCGCCC
ACAGTCCCCG
AGAAGTTGGG
GGGAGGGGTC
GGCAATTGAT
CCGGTGCCTA
GAGAAGGTGG
CGCGGGGTAA
ACTGGGAAAG
TGATGTCGTG
TACTGGCTCC
GCCTTTTTCC
CGAGGGTGGG
GGAGAACCGT
ATATAAGTGC
AGTAGTCGCC
GTGAACGTTC
TTTTTCGCAA
CGGGTTTGCC
GCCAGAACAC
AGGACCGGTT
CTAGAGCGCT
GCCACCATGG
ACAAGAAGTA
CAGCATCGGC
CTGGACATCG
GCACCAACTC
TGTGGGCTGG
GCCGTGATCA
CCGACGAGTA
CAAGGTGCCC
AGCAAGAAAT
TCAAGGTGCT
GGGCAACACC
GACCGGCACA
GCATCAAGAA
GAACCTGATC
GGAGCCCTGC
TGTTCGACAG
CGGCGAAACA
GCCGAGGCCA
CCCGGCTGAA
GAGAACCGCC
AGAAGAAGAT
ACACCAGACG
GAAGAACCGG
ATCTGCTATC
TGCAAGAGAT
CTTCAGCAAC
GAGATGGCCA
AGGTGGACGA
CAGCTTCTTC
CACAGACTGG
AAGAGTCCTT
CCTGGTGGAA
GAGGATAAGA
AGCACGAGCG
GCACCCCATC
TTCGGCAACA
TCGTGGACGA
GGTGGCCTAC
CACGAGAAGT
ACCCCACCAT
CTACCACCTG
AGAAAGAAAC
TGGTGGACAG
CACCGACAAG
GCCGACCTGC
GGCTGATCTA
TCTGGCCCTG
GCCCACATGA
TCAAGTTCCG
GGGCCACTTC
CTGATCGAGG
GCGACCTGAA
CCCCGACAAC
AGCGACGTGG
ACAAGCTGTT
CATCCAGCTG
GTGCAGACCT
ACAACCAGCT
GTTCGAGGAA
AACCCCATCA
ACGCCAGCGG
CGTGGACGCC
AAGGCCATCC
TGTCTGCCAG
ACTGAGCAAG
AGCAGACGGC
TGGAAAATCT
GATCGCCCAG
CTGCCCGGCG
AGAAGAAGAA
TGGCCTGTTC
GGAAACCTGA
TTGCCCTGAG
CCTGGGCCTG
ACCCCCAACT
TCAAGAGCAA
CTTCGACCTG
GCCGAGGATG
CCAAACTGCA
GCTGAGCAAG
GACACCTACG
ACGACGACCT
GGACAACCTG
CTGGCCCAGA
TCGGCGACCA
GTACGCCGAC
CTGTTTCTGG
CCGCCAAGAA
CCTGTCCGAC
GCCATCCTGC
TGAGCGACAT
CCTGAGAGTG
AACACCGAGA
TCACCAAGGC
CCCCCTGAGC
GCCTCTATGA
TCAAGAGATA
CGACGAGCAC
CACCAGGACC
TGACCCTGCT
GAAAGCTCTC
GTGCGGCAGC
AGCTGCCTGA
GAAGTACAAA
GAGATTTTCT
TCGACCAGAG
CAAGAACGGC
TACGCCGGCT
ACATTGACGG
CGGAGCCAGC
CAGGAAGAGT
TCTACAAGTT
CATCAAGCCC
ATCCTGGAAA
AGATGGACGG
CACCGAGGAA
CTGCTCGTGA
AGCTGAACAG
AGAGGACCTG
CTGCGGAAGC
AGCGGACCTT
CGACAACGGC
AGCATCCCCC
ACCAGATCCA
CCTGGGAGAG
CTGCACGCCA
TTCTGCGGCG
GCAGGAAGAT
TTTTACCCAT
TCCTGAAGGA
CAACCGGGAA
AAGATCGAGA
AGATCCTGAC
CTTCCGCATC
CCCTACTACG
TGGGCCCTCT
GGCCAGGGGA
AACAGCAGAT
TCGCCTGGAT
GACCAGAAAG
AGCGAGGAAA
CCATCACCCC
CTGGAACTTC
GAGGAAGTGG
TGGACAAGGG
CGCTTCCGCC
CAGAGCTTCA
TCGAGCGGAT
GACCAACTTC
GATAAGAACC
TGCCCAACGA
GAAGGTGCTG
CCCAAGCACA
GCCTGCTGTA
CGAGTACTTC
ACCGTGTATA
ACGAGCTGAC
CAAAGTGAAA
TACGTGACCG
AGGGAATGAG
AAAGCCCGCC
TTCCTGAGCG
GCGAGCAGAA
AAAGGCCATC
GTGGACCTGC
TGTTCAAGAC
CAACCGGAAA
GTGACCGTGA
AGCAGCTGAA
AGAGGACTAC
TTCAAGAAAA
TCGAGTGCTT
CGACTCCGTG
GAAATCTCCG
GCGTGGAAGA
TCGGTTCAAC
GCCTCCCTGG
GCACATACCA
CGATCTGCTG
AAAATTATCA
AGGACAAGGA
CTTCCTGGAC
AATGAGGAAA
ACGAGGACAT
TCTGGAAGAT
ATCGTGCTGA
CCCTGACACT
GTTTGAGGAC
AGAGAGATGA
TCGAGGAACG
GCTGAAAACC
TATGCCCACC
TGTTCGACGA
CAAAGTGATG
AAGCAGCTGA
AGCGGCGGAG
ATACACCGGC
TGGGGCAGGC
TGAGCCGGAA
GCTGATCAAC
GGCATCCGGG
ACAAGCAGTC
CGGCAAGACA
ATCCTGGATT
TCCTGAAGTC
CGACGGCTTC
GCCAACAGAA
ACTTCATGCA
GCTGATCCAC
GACGACAGCC
TGACCTTTAA
AGAGGACATC
CAGAAAGCCC
AGGTGTCCGG
CCAGGGCGAT
AGCCTGCACG
AGCACATTGC
CAATCTGGCC
GGCAGCCCCG
CCATTAAGAA
GGGCATCCTG
CAGACAGTGA
AGGTGGTGGA
CGAGCTCGTG
AAAGTGATGG
GCCGGCACAA
GCCCGAGAAC
ATCGTGATCG
AAATGGCCAG
AGAGAACCAG
ACCACCCAGA
AGGGACAGAA
GAACAGCCGC
GAGAGAATGA
AGCGGATCGA
AGAGGGCATC
AAAGAGCTGG
GCAGCCAGAT
CCTGAAAGAA
CACCCCGTGG
AAAACACCCA
GCTGCAGAAC
GAGAAGCTGT
ACCTGTACTA
CCTGCAGAAT
GGGCGGGATA
TGTACGTGGA
CCAGGAACTG
GACATCAACC
GGCTGTCCGA
CTACGATGTG
GACCATATCG
TGCCTCAGAG
CTTTCTGAAG
GACGACTCCA
TCGACAACAA
GGTGCTGACC
AGAAGCGACA
AGAACCGGGG
CAAGAGCGAC
AACGTGCCCT
CCGAAGAGGT
CGTGAAGAAG
ATGAAGAACT
ACTGGCGGCA
GCTGCTGAAC
GCCAAGCTGA
TTACCCAGAG
AAAGTTCGAC
AATCTGACCA
AGGCCGAGAG
AGGCGGCCTG
AGCGAACTGG
ATAAGGCCGG
CTTCATCAAG
AGACAGCTGG
TGGAAACCCG
GCAGATCACA
AAGCACGTGG
CACAGATCCT
GGACTCCCGG
ATGAACACTA
AGTACGACGA
GAATGACAAG
CTGATCCGGG
AAGTGAAAGT
GATCACCCTG
AAGTCCAAGC
TGGTGTCCGA
TTTCCGGAAG
GATTTCCAGT
TTTACAAAGT
GCGCGAGATC
AACAACTACC
ACCACGCCCA
CGACGCCTAC
CTGAACGCCG
TCGTGGGAAC
CGCCCTGATC
AAAAAGTACC
CTAAGCTGGA
AAGCGAGTTC
GTGTACGGCG
ACTACAAGGT
GTACGACGTG
CGGAAGATGA
TCGCCAAGAG
CGAGCAGGAA
ATCGGCAAGG
CTACCGCCAA
GTACTTCTTC
TACAGCAACA
TCATGAACTT
TTTCAAGACC
GAGATTACCC
TGGCCAACGG
CGAGATCCGG
AAGCGGCCTC
TGATCGAGAC
AAACGGCGAA
ACCGGGGAGA
TCGTGTGGGA
TAAGGGCCGG
GATTTTGCCA
CCGTGCGGAA
AGTGCTGAGC
ATGCCCCAAG
TGAATATCGT
GAAAAAGACC
GAGGTGCAGA
CAGGCGGCTT
CAGCAAAGAG
TCTATCCTGC
CCAAGAGGAA
CAGCGATAAG
CTGATCGCCA
GAAAGAAGGA
CTGGGACCCT
AAGAAGTACG
GCGGCTTCGA
CAGCCCCACC
GTGGCCTATT
CTGTGCTGGT
GGTGGCCAAA
GTGGAAAAGG
GCAAGTCCAA
GAAACTGAAG
AGTGTGAAAG
AGCTGCTGGG
GATCACCATC
ATGGAAAGAA
GCAGCTTCGA
GAAGAATCCC
ATCGACTTTC
TGGAAGCCAA
GGGCTACAAA
GAAGTGAAAA
AGGACCTGAT
CATCAAGCTG
CCTAAGTACT
CCCTGTTCGA
GCTGGAAAAC
GGCCGGAAGA
GAATGCTGGC
CTCTGCCGGC
GAACTGCAGA
AGGGAAACGA
ACTGGCCCTG
CCCTCCAAAT
ATGTGAACTT
CCTGTACCTG
GCCAGCCACT
ATGAGAAGCT
GAAGGGCTCC
CCCGAGGATA
ATGAGCAGAA
ACAGCTGTTT
GTGGAACAGC
ACAAGCACTA
CCTGGACGAG
ATCATCGAGC
AGATCAGCGA
GTTCTCCAAG
AGAGTGATCC
TGGCCGACGC
TAATCTGGAC
AAAGTGCTGT
CCGCCTACAA
CAAGCACCGG
GATAAGCCCA
TCAGAGAGCA
GGCCGAGAAT
ATCATCCACC
TGTTTACCCT
GACCAATCTG
GGAGCCCCTG
CCGCCTTCAA
GTACTTTGAC
ACCACCATCG
ACCGGAAGAG
GTACACCAGC
ACCAAAGAGG
TGCTGGACGC
CACCCTGATC
CACCAGAGCA
TCACCGGCCT
GTACGAGACA
CGGATCGACC
TGTCTCAGCT
GGGAGGCGAC
AAGCGACCTG
CCGCCACAAA
GAAGGCTGGA
CAGGCTAAGA
AGAAGAAAGA
TTACAAAGAC
GATGACGATA
AGGGATCCGG
CGCAACAAAC
TTCTCTCTGC
TGAAACAAGC
CGGAGATGTC
GAAGAGAATC
CTGGACCGAT
GGTGTCTAAG
GGCGAAGAGC
TGTTCACCGG
GGTGGTGCCC
ATCCTGGTCG
AGCTGGACGG
CGACGTAAAC
GGCCACAAGT
TCAGCGTGTC
CGGCGAGGGC
GAGGGCGATG
CCACCTACGG
CAAGCTGACC
CTGAAGTTCA
TCTGCACCAC
CGGCAAGCTG
CCCGTGCCCT
GGCCCACCCT
CGTGACCACC
CTGACCTACG
GCGTGCAGTG
CTTCAGCCGC
TACCCCGACC
ACATGAAGCA
GCACGACTTC
TTCAAGTCCG
CCATGCCCGA
AGGCTACGTC
CAGGAGCGCA
CCATCTTCTT
CAAGGACGAC
GGCAACTACA
AGACCCGCGC
CGAGGTGAAG
TTCGAGGGCG
ACACCCTGGT
GAACCGCATC
GAGCTGAAGG
GCATCGACTT
CAAGGAGGAC
GGCAACATCC
TGGGGCACAA
GCTGGAGTAC
AACTACAACA
GCCACAACGT
CTATATCATG
GCCGACAAGC
AGAAGAACGG
CATCAAGGTG
AACTTCAAGA
TCCGCCACAA
CATCGAGGAC
GGCAGCGTGC
AGCTCGCCGA
CCACTACCAG
CAGAACACCC
CCATCGGCGA
CGGCCCCGTG
CTGCTGCCCG
ACAACCACTA
CCTGAGCACC
CAGTCCGCCC
TGAGCAAAGA
CCCCAACGAG
AAGCGCGATC
ACATGGTCCT
GCTGGAGTTC
GTGACCGCCG
CCGGGATCAC
TCTCGGCATG
GACGAGCTGT
ACAAGGGATC
CGGCGCAACA
AACTTCTCTC
TGCTGAAACA
AGCCGGAGAT
GTCGAAGAGA
ATCCTGGACC
GATTGAACAA
GATGGATTGC
ACGCAGGTTC
TCCGGCCGCT
TGGGTGGAGA
GGCTATTCGG
CTATGACTGG
GCACAACAGA
CAATCGGCTG
CTCTGATGCC
GCCGTGTTCC
GGCTGTCAGC
GCAGGGGCGC
CCGGTTCTTT
TTGTCAAGAC
CGACCTGTCC
GGTGCCCTGA
ATGAACTGCA
GGACGAGGCA
GCGCGGCTAT
CGTGGCTGGC
CACGACGGGC
GTTCCTTGCG
CAGCTGTGCT
CGACGTTGTC
ACTGAAGCGG
GAAGGGACTG
GCTGCTATTG
GGCGAAGTGC
CGGGGCAGGA
TCTCCTGTCA
TCTCACCTTG
CTCCTGCCGA
GAAAGTATCC
ATCATGGCTG
ATGCAATGCG
GCGGCTGCAT
ACGCTTGATC
CGGCTACCTG
CCCATTCGAC
CACCAAGCGA
AACATCGCAT
CGAGCGAGCA
CGTACTCGGA
TGGAAGCCGG
TCTTGTCGAT
CAGGATGATC
TGGACGAAGA
GCATCAGGGG
CTCGCGCCAG
CCGAACTGTT
CGCCAGGCTC
AAGGCGCGCA
TGCCCGACGG
CGAGGATCTC
GTCGTGACCC
ATGGCGATGC
CTGCTTGCCG
AATATCATGG
TGGAAAATGG
CCGCTTTTCT
GGATTCATCG
ACTGTGGCCG
GCTGGGTGTG
GCGGACCGCT
ATCAGGACAT
AGCGTTGGCT
ACCCGTGATA
TTGCTGAAGA
GCTTGGCGGC
GAATGGGCTG
ACCGCTTCCT
CGTGCTTTAC
GGTATCGCCG
CTCCCGATTC
GCAGCGCATC
GCCTTCTATC
GCCTTCTTGA
CGAGTTCTTC
TGACTCGAGT
ACGCGTTAAG
TCGACAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCGTCGAC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
GGCCCGTTTA
AACCCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGACAA
TAGCAGGCAT
GCTGGGGATG
CGGTGGGCTC
TATGGCTTCT
GAGGCGGAAA
GAACCAGCTG
GGGCTCTAGG
GGGTATCCCC
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTA
ATTCTGTGGA
ATCACGTGCT
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCG
ACGGATCGGG
AGATCTCCCG
ATCCCCTATG
GTGCACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGTATC
TGCTCCCTGC
TTGTGTGTTG
GAGGTCGCTG
AGTAGTGCGC
GAGCAAAATT
TAAGCTACAA
CAAGGCAAGG
CTTGACCGAC
AATTGCATGA
AGAATCTGCT
TAGGGTTAGG
CGTTTTGCGC
TGCTTCGCGA
TGTACGGGCC
AGATATACGC
GTTGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTA
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTGATGC
GGTTTTGGCA
GTACATCAAT
GGGCGTGGAT
AGCGGTTTGA
CTCACGGGGA
TTTCCAAGTC
TCCACCCCAT
TGACGTCAAT
GGGAGTTTGT
TTTGGCACCA
AAATCAACGG
GACTTTCCAA
AATGTCGTAA
CAACTCCGCC
CCATTGACGC
AAATGGGCGG
TAGGCGTGTA
CGGTGGGAGG
TCTATATAAG
CAGCGCGTTT
TGCCTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTGGCGCCC
GAACAGGGAC
TTGAAAGCGA
AAGGGAAACC
AGAGGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCGCTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCGGCA
CTGCGTGCGC
CAATTCTGCA
GACAAATGGC
AGTATTCATC
CACAATTTTA
AAAGAAAAGG
GGGGATTGGG
GGGTACAGTG
CAGGGGAAAG
AATAGTAGAC
ATAATAGCAA
CAGACATACA
AACTAAAGAA
TTACAAAAAC
AAATTACAAA
AATTCAAAAT
TTTCGGGTTT
ATTACAGGGA
CAGCAGAGAT
CCAGTTTGGT
TAATTAAGGT
ACC