名称: | pLV3-U6-VHL(human)-sgRNA1-HIF1A(human)-sgRNA2-Cas9-EGFP-Puro |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-U6-VHL(human)-sgRNA1-Cas9-EGFP-Puro |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTAGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
CCCGGAAGAG
TCCGGCCCGG
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGAATTCTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTGAGGGCCT
ATTTCCCATG
ATTCCTTCAT
ATTTGCATAT
ACGATACAAG
GCTGTTAGAG
AGATAATTGG
AATTAATTTG
ACTGTAAACA
CAAAGATATT
AGTACAAAAT
ACGTGACGTA
GAAAGTAATA
ATTTCTTGGG
TAGTTTGCAG
TTTTAAAATT
ATGTTTTAAA
ATGGACTATC
ATATGCTTAC
CGTAACTTGA
AAGTATTTCG
ATTTCTTGGC
TTTATATATC
TTGTGGAAAG
GACGAAACAC
CGGATGGTAA
GCCTCATCAC
AGGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTATCA
ACTTGAAAAA
GTGGCACCGA
GTCGGTGCTT
TTTTGGCTAG
CTAGGTCTTG
AAAGGAGTGG
GAATTGGCTC
CGGTGCCCGT
CAGTGGGCAG
AGCGCACATC
GCCCACAGTC
CCCGAGAAGT
TGGGGGGAGG
GGTCGGCAAT
TGATCCGGTG
CCTAGAGAAG
GTGGCGCGGG
GTAAACTGGG
AAAGTGATGT
CGTGTACTGG
CTCCGCCTTT
TTCCCGAGGG
TGGGGGAGAA
CCGTATATAA
GTGCAGTAGT
CGCCGTGAAC
GTTCTTTTTC
GCAACGGGTT
TGCCGCCAGA
ACACAGGACC
GGTTCTAGAG
CGCTGCCACC
ATGGACAAGA
AGTACAGCAT
CGGCCTGGAC
ATCGGCACCA
ACTCTGTGGG
CTGGGCCGTG
ATCACCGACG
AGTACAAGGT
GCCCAGCAAG
AAATTCAAGG
TGCTGGGCAA
CACCGACCGG
CACAGCATCA
AGAAGAACCT
GATCGGAGCC
CTGCTGTTCG
ACAGCGGCGA
AACAGCCGAG
GCCACCCGGC
TGAAGAGAAC
CGCCAGAAGA
AGATACACCA
GACGGAAGAA
CCGGATCTGC
TATCTGCAAG
AGATCTTCAG
CAACGAGATG
GCCAAGGTGG
ACGACAGCTT
CTTCCACAGA
CTGGAAGAGT
CCTTCCTGGT
GGAAGAGGAT
AAGAAGCACG
AGCGGCACCC
CATCTTCGGC
AACATCGTGG
ACGAGGTGGC
CTACCACGAG
AAGTACCCCA
CCATCTACCA
CCTGAGAAAG
AAACTGGTGG
ACAGCACCGA
CAAGGCCGAC
CTGCGGCTGA
TCTATCTGGC
CCTGGCCCAC
ATGATCAAGT
TCCGGGGCCA
CTTCCTGATC
GAGGGCGACC
TGAACCCCGA
CAACAGCGAC
GTGGACAAGC
TGTTCATCCA
GCTGGTGCAG
ACCTACAACC
AGCTGTTCGA
GGAAAACCCC
ATCAACGCCA
GCGGCGTGGA
CGCCAAGGCC
ATCCTGTCTG
CCAGACTGAG
CAAGAGCAGA
CGGCTGGAAA
ATCTGATCGC
CCAGCTGCCC
GGCGAGAAGA
AGAATGGCCT
GTTCGGAAAC
CTGATTGCCC
TGAGCCTGGG
CCTGACCCCC
AACTTCAAGA
GCAACTTCGA
CCTGGCCGAG
GATGCCAAAC
TGCAGCTGAG
CAAGGACACC
TACGACGACG
ACCTGGACAA
CCTGCTGGCC
CAGATCGGCG
ACCAGTACGC
CGACCTGTTT
CTGGCCGCCA
AGAACCTGTC
CGACGCCATC
CTGCTGAGCG
ACATCCTGAG
AGTGAACACC
GAGATCACCA
AGGCCCCCCT
GAGCGCCTCT
ATGATCAAGA
GATACGACGA
GCACCACCAG
GACCTGACCC
TGCTGAAAGC
TCTCGTGCGG
CAGCAGCTGC
CTGAGAAGTA
CAAAGAGATT
TTCTTCGACC
AGAGCAAGAA
CGGCTACGCC
GGCTACATTG
ACGGCGGAGC
CAGCCAGGAA
GAGTTCTACA
AGTTCATCAA
GCCCATCCTG
GAAAAGATGG
ACGGCACCGA
GGAACTGCTC
GTGAAGCTGA
ACAGAGAGGA
CCTGCTGCGG
AAGCAGCGGA
CCTTCGACAA
CGGCAGCATC
CCCCACCAGA
TCCACCTGGG
AGAGCTGCAC
GCCATTCTGC
GGCGGCAGGA
AGATTTTTAC
CCATTCCTGA
AGGACAACCG
GGAAAAGATC
GAGAAGATCC
TGACCTTCCG
CATCCCCTAC
TACGTGGGCC
CTCTGGCCAG
GGGAAACAGC
AGATTCGCCT
GGATGACCAG
AAAGAGCGAG
GAAACCATCA
CCCCCTGGAA
CTTCGAGGAA
GTGGTGGACA
AGGGCGCTTC
CGCCCAGAGC
TTCATCGAGC
GGATGACCAA
CTTCGATAAG
AACCTGCCCA
ACGAGAAGGT
GCTGCCCAAG
CACAGCCTGC
TGTACGAGTA
CTTCACCGTG
TATAACGAGC
TGACCAAAGT
GAAATACGTG
ACCGAGGGAA
TGAGAAAGCC
CGCCTTCCTG
AGCGGCGAGC
AGAAAAAGGC
CATCGTGGAC
CTGCTGTTCA
AGACCAACCG
GAAAGTGACC
GTGAAGCAGC
TGAAAGAGGA
CTACTTCAAG
AAAATCGAGT
GCTTCGACTC
CGTGGAAATC
TCCGGCGTGG
AAGATCGGTT
CAACGCCTCC
CTGGGCACAT
ACCACGATCT
GCTGAAAATT
ATCAAGGACA
AGGACTTCCT
GGACAATGAG
GAAAACGAGG
ACATTCTGGA
AGATATCGTG
CTGACCCTGA
CACTGTTTGA
GGACAGAGAG
ATGATCGAGG
AACGGCTGAA
AACCTATGCC
CACCTGTTCG
ACGACAAAGT
GATGAAGCAG
CTGAAGCGGC
GGAGATACAC
CGGCTGGGGC
AGGCTGAGCC
GGAAGCTGAT
CAACGGCATC
CGGGACAAGC
AGTCCGGCAA
GACAATCCTG
GATTTCCTGA
AGTCCGACGG
CTTCGCCAAC
AGAAACTTCA
TGCAGCTGAT
CCACGACGAC
AGCCTGACCT
TTAAAGAGGA
CATCCAGAAA
GCCCAGGTGT
CCGGCCAGGG
CGATAGCCTG
CACGAGCACA
TTGCCAATCT
GGCCGGCAGC
CCCGCCATTA
AGAAGGGCAT
CCTGCAGACA
GTGAAGGTGG
TGGACGAGCT
CGTGAAAGTG
ATGGGCCGGC
ACAAGCCCGA
GAACATCGTG
ATCGAAATGG
CCAGAGAGAA
CCAGACCACC
CAGAAGGGAC
AGAAGAACAG
CCGCGAGAGA
ATGAAGCGGA
TCGAAGAGGG
CATCAAAGAG
CTGGGCAGCC
AGATCCTGAA
AGAACACCCC
GTGGAAAACA
CCCAGCTGCA
GAACGAGAAG
CTGTACCTGT
ACTACCTGCA
GAATGGGCGG
GATATGTACG
TGGACCAGGA
ACTGGACATC
AACCGGCTGT
CCGACTACGA
TGTGGACCAT
ATCGTGCCTC
AGAGCTTTCT
GAAGGACGAC
TCCATCGACA
ACAAGGTGCT
GACCAGAAGC
GACAAGAACC
GGGGCAAGAG
CGACAACGTG
CCCTCCGAAG
AGGTCGTGAA
GAAGATGAAG
AACTACTGGC
GGCAGCTGCT
GAACGCCAAG
CTGATTACCC
AGAGAAAGTT
CGACAATCTG
ACCAAGGCCG
AGAGAGGCGG
CCTGAGCGAA
CTGGATAAGG
CCGGCTTCAT
CAAGAGACAG
CTGGTGGAAA
CCCGGCAGAT
CACAAAGCAC
GTGGCACAGA
TCCTGGACTC
CCGGATGAAC
ACTAAGTACG
ACGAGAATGA
CAAGCTGATC
CGGGAAGTGA
AAGTGATCAC
CCTGAAGTCC
AAGCTGGTGT
CCGATTTCCG
GAAGGATTTC
CAGTTTTACA
AAGTGCGCGA
GATCAACAAC
TACCACCACG
CCCACGACGC
CTACCTGAAC
GCCGTCGTGG
GAACCGCCCT
GATCAAAAAG
TACCCTAAGC
TGGAAAGCGA
GTTCGTGTAC
GGCGACTACA
AGGTGTACGA
CGTGCGGAAG
ATGATCGCCA
AGAGCGAGCA
GGAAATCGGC
AAGGCTACCG
CCAAGTACTT
CTTCTACAGC
AACATCATGA
ACTTTTTCAA
GACCGAGATT
ACCCTGGCCA
ACGGCGAGAT
CCGGAAGCGG
CCTCTGATCG
AGACAAACGG
CGAAACCGGG
GAGATCGTGT
GGGATAAGGG
CCGGGATTTT
GCCACCGTGC
GGAAAGTGCT
GAGCATGCCC
CAAGTGAATA
TCGTGAAAAA
GACCGAGGTG
CAGACAGGCG
GCTTCAGCAA
AGAGTCTATC
CTGCCCAAGA
GGAACAGCGA
TAAGCTGATC
GCCAGAAAGA
AGGACTGGGA
CCCTAAGAAG
TACGGCGGCT
TCGACAGCCC
CACCGTGGCC
TATTCTGTGC
TGGTGGTGGC
CAAAGTGGAA
AAGGGCAAGT
CCAAGAAACT
GAAGAGTGTG
AAAGAGCTGC
TGGGGATCAC
CATCATGGAA
AGAAGCAGCT
TCGAGAAGAA
TCCCATCGAC
TTTCTGGAAG
CCAAGGGCTA
CAAAGAAGTG
AAAAAGGACC
TGATCATCAA
GCTGCCTAAG
TACTCCCTGT
TCGAGCTGGA
AAACGGCCGG
AAGAGAATGC
TGGCCTCTGC
CGGCGAACTG
CAGAAGGGAA
ACGAACTGGC
CCTGCCCTCC
AAATATGTGA
ACTTCCTGTA
CCTGGCCAGC
CACTATGAGA
AGCTGAAGGG
CTCCCCCGAG
GATAATGAGC
AGAAACAGCT
GTTTGTGGAA
CAGCACAAGC
ACTACCTGGA
CGAGATCATC
GAGCAGATCA
GCGAGTTCTC
CAAGAGAGTG
ATCCTGGCCG
ACGCTAATCT
GGACAAAGTG
CTGTCCGCCT
ACAACAAGCA
CCGGGATAAG
CCCATCAGAG
AGCAGGCCGA
GAATATCATC
CACCTGTTTA
CCCTGACCAA
TCTGGGAGCC
CCTGCCGCCT
TCAAGTACTT
TGACACCACC
ATCGACCGGA
AGAGGTACAC
CAGCACCAAA
GAGGTGCTGG
ACGCCACCCT
GATCCACCAG
AGCATCACCG
GCCTGTACGA
GACACGGATC
GACCTGTCTC
AGCTGGGAGG
CGACAAGCGA
CCTGCCGCCA
CAAAGAAGGC
TGGACAGGCT
AAGAAGAAGA
AAGATTACAA
AGACGATGAC
GATAAGGGAT
CCGGCGCAAC
AAACTTCTCT
CTGCTGAAAC
AAGCCGGAGA
TGTCGAAGAG
AATCCTGGAC
CGATGGTGTC
TAAGGGCGAA
GAGCTGTTCA
CCGGGGTGGT
GCCCATCCTG
GTCGAGCTGG
ACGGCGACGT
AAACGGCCAC
AAGTTCAGCG
TGTCCGGCGA
GGGCGAGGGC
GATGCCACCT
ACGGCAAGCT
GACCCTGAAG
TTCATCTGCA
CCACCGGCAA
GCTGCCCGTG
CCCTGGCCCA
CCCTCGTGAC
CACCCTGACC
TACGGCGTGC
AGTGCTTCAG
CCGCTACCCC
GACCACATGA
AGCAGCACGA
CTTCTTCAAG
TCCGCCATGC
CCGAAGGCTA
CGTCCAGGAG
CGCACCATCT
TCTTCAAGGA
CGACGGCAAC
TACAAGACCC
GCGCCGAGGT
GAAGTTCGAG
GGCGACACCC
TGGTGAACCG
CATCGAGCTG
AAGGGCATCG
ACTTCAAGGA
GGACGGCAAC
ATCCTGGGGC
ACAAGCTGGA
GTACAACTAC
AACAGCCACA
ACGTCTATAT
CATGGCCGAC
AAGCAGAAGA
ACGGCATCAA
GGTGAACTTC
AAGATCCGCC
ACAACATCGA
GGACGGCAGC
GTGCAGCTCG
CCGACCACTA
CCAGCAGAAC
ACCCCCATCG
GCGACGGCCC
CGTGCTGCTG
CCCGACAACC
ACTACCTGAG
CACCCAGTCC
GCCCTGAGCA
AAGACCCCAA
CGAGAAGCGC
GATCACATGG
TCCTGCTGGA
GTTCGTGACC
GCCGCCGGGA
TCACTCTCGG
CATGGACGAG
CTGTACAAGG
AGGGCAGAGG
AAGTCTTCTA
ACATGCGGTG
ACGTGGAGGA
GAATCCCGGC
CCTATGACCG
AGTACAAGCC
CACGGTGCGC
CTCGCCACCC
GCGACGACGT
CCCCAGGGCC
GTACGCACCC
TCGCCGCCGC
GTTCGCCGAC
TACCCCGCCA
CGCGCCACAC
CGTCGATCCG
GACCGCCACA
TCGAGCGGGT
CACCGAGCTG
CAAGAACTCT
TCCTCACGCG
CGTCGGGCTC
GACATCGGCA
AGGTGTGGGT
CGCGGACGAC
GGCGCCGCGG
TGGCGGTCTG
GACCACGCCG
GAGAGCGTCG
AAGCGGGGGC
GGTGTTCGCC
GAGATCGGCC
CGCGCATGGC
CGAGTTGAGC
GGTTCCCGGC
TGGCCGCGCA
GCAACAGATG
GAAGGCCTCC
TGGCGCCGCA
CCGGCCCAAG
GAGCCCGCGT
GGTTCCTGGC
CACCGTCGGA
GTCTCGCCCG
ACCACCAGGG
CAAGGGTCTG
GGCAGCGCCG
TCGTGCTCCC
CGGAGTGGAG
GCGGCCGAGC
GCGCCGGGGT
GCCCGCCTTC
CTGGAGACCT
CCGCGCCCCG
CAACCTCCCC
TTCTACGAGC
GGCTCGGCTT
CACCGTCACC
GCCGACGTCG
AGGTGCCCGA
AGGACCGCGC
ACCTGGTGCA
TGACCCGCAA
GCCCGGTGCC
TGACTCGAGT
ACGCGTTAAG
TCGACAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCGTCGAC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
GGCCCGTTTA
AACCCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGACAA
TAGCAGGCAT
GCTGGGGATG
CGGTGGGCTC
TATGGCTTCT
GAGGCGGAAA
GAACCAGCTG
GGGCTCTAGG
GGGTATCCCC
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTA
ATTCTGTGGA
ATCACGTGCT
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCG
ACGGATCGGG
AGATCTCCCG
ATCCCCTATG
GTGCACTCTC
AGTACAATCT
GCTCTGATGC
CGCATAGTTA
AGCCAGTATC
TGCTCCCTGC
TTGTGTGTTG
GAGGTCGCTG
AGTAGTGCGC
GAGCAAAATT
TAAGCTACAA
CAAGGCAAGG
CTTGACCGAC
AATTGCATGA
AGAATCTGCT
TAGGGTTAGG
CGTTTTGCGC
TGCTTCGCGA
TGTACGGGCC
AGATATACGC
GTTGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTA
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTGATGC
GGTTTTGGCA
GTACATCAAT
GGGCGTGGAT
AGCGGTTTGA
CTCACGGGGA
TTTCCAAGTC
TCCACCCCAT
TGACGTCAAT
GGGAGTTTGT
TTTGGCACCA
AAATCAACGG
GACTTTCCAA
AATGTCGTAA
CAACTCCGCC
CCATTGACGC
AAATGGGCGG
TAGGCGTGTA
CGGTGGGAGG
TCTATATAAG
CAGCGCGTTT
TGCCTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTGGCGCCC
GAACAGGGAC
TTGAAAGCGA
AAGGGAAACC
AGAGGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCGCTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCGGCA
CTGCGTGCGC
CAATTCTGCA
GACAAATGGC
AGTATTCATC
CACAATTTTA
AAAGAAAAGG
GGGGATTGGG
GGGTACAGTG
CAGGGGAAAG
AATAGTAGAC
ATAATAGCAA
CAGACATACA
AACTAAAGAA
TTACAAAAAC
AAATTACAAA
AATTCAAAAT
TTTCGGGTTT
ATTACAGGGA
CAGCAGAGAT
CCAGTTTGGT
TAATTAAGGT
ACC