产品货号：P63916
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV3-CMV-fLuc-CHL1(human)-Puro |
|---|---|
| 别称: | NM_006614.4;CALL; L1CAM2 |
| 基因长: | 3675bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | Fluc |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV3-CMV-Fluc-MCS-EF1a-Puro |
| 正向引物: | Luc-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTGC
CACCATGGAA
GACGCCAAAA
ACATAAAGAA
AGGCCCGGCG
CCATTCTATC
CGCTAGAGGA
TGGAACCGCT
GGAGAGCAAC
TGCATAAGGC
TATGAAGAGA
TACGCCCTGG
TTCCTGGAAC
AATTGCTTTT
ACAGATGCAC
ATATCGAGGT
GAACATCACG
TACGCGGAAT
ACTTCGAAAT
GTCCGTTCGG
TTGGCAGAAG
CTATGAAACG
ATATGGGCTG
AATACAAATC
ACAGAATCGT
CGTATGCAGT
GAAAACTCTC
TTCAATTCTT
TATGCCGGTG
TTGGGCGCGT
TATTTATCGG
AGTTGCAGTT
GCGCCCGCGA
ACGACATTTA
TAATGAACGT
GAATTGCTCA
ACAGTATGAA
CATTTCGCAG
CCTACCGTAG
TGTTTGTTTC
CAAAAAGGGG
TTGCAAAAAA
TTTTGAACGT
GCAAAAAAAA
TTACCAATAA
TCCAGAAAAT
TATTATCATG
GATTCTAAAA
CGGATTACCA
GGGATTTCAG
TCGATGTACA
CGTTCGTCAC
ATCTCATCTA
CCTCCCGGTT
TTAATGAATA
CGATTTTGTA
CCAGAGTCCT
TTGATCGTGA
CAAAACAATT
GCACTGATAA
TGAACTCCTC
TGGATCTACT
GGGTTACCTA
AGGGTGTGGC
CCTTCCGCAT
AGAACTGCCT
GCGTCAGATT
CTCGCATGCC
AGAGATCCTA
TTTTTGGCAA
TCAAATCATT
CCGGATACTG
CGATTTTAAG
TGTTGTTCCA
TTCCATCACG
GTTTTGGAAT
GTTTACTACA
CTCGGATATT
TGATATGTGG
ATTTCGAGTC
GTCTTAATGT
ATAGATTTGA
AGAAGAGCTG
TTTTTACGAT
CCCTTCAGGA
TTACAAAATT
CAAAGTGCGT
TGCTAGTACC
AACCCTATTT
TCATTCTTCG
CCAAAAGCAC
TCTGATTGAC
AAATACGATT
TATCTAATTT
ACACGAAATT
GCTTCTGGGG
GCGCACCTCT
TTCGAAAGAA
GTCGGGGAAG
CGGTTGCAAA
ACGCTTCCAT
CTTCCAGGGA
TACGACAAGG
ATATGGGCTC
ACTGAGACTA
CATCAGCTAT
TCTGATTACA
CCCGAGGGGG
ATGATAAACC
GGGCGCGGTC
GGTAAAGTTG
TTCCATTTTT
TGAAGCGAAG
GTTGTGGATC
TGGATACCGG
GAAAACGCTG
GGCGTTAATC
AGAGAGGCGA
ATTATGTGTC
AGAGGACCTA
TGATTATGTC
CGGTTATGTA
AACAATCCGG
AAGCGACCAA
CGCCTTGATT
GACAAGGATG
GATGGCTACA
TTCTGGAGAC
ATAGCTTACT
GGGACGAAGA
CGAACACTTC
TTCATAGTTG
ACCGCTTGAA
GTCTTTAATT
AAATACAAAG
GATACCAGGT
GGCCCCCGCT
GAATTGGAGT
CGATATTGTT
ACAACACCCC
AACATCTTCG
ACGCGGGCGT
GGCAGGTCTT
CCCGACGATG
ACGCCGGTGA
ACTTCCCGCC
GCCGTTGTTG
TTTTGGAGCA
CGGAAAGACG
ATGACGGAAA
AAGAGATCGT
GGATTACGTC
GCCAGTCAAG
TAACAACCGC
GAAAAAGTTG
CGCGGAGGAG
TTGTGTTTGT
GGACGAAGTA
CCGAAAGGTC
TTACCGGAAA
ACTCGACGCA
AGAAAAATCA
GAGAGATCCT
CATAAAGGCC
AAGAAGGGCG
GAAAGTCCAA
ATTGGAATTC
ATGGAGCCGC
TTTTACTTGG
AAGAGGACTA
ATCGTATATC
TAATGTTCCT
CCTGTTAAAA
TTCTCAAAAG
CAATTGAAAT
ACCATCTTCA
GTTCAACAGG
TTCCAACAAT
CATAAAACAG
TCAAAAGTCC
AAGTTGCCTT
TCCCTTCGAT
GAGTATTTTC
AAATTGAATG
TGAAGCTAAA
GGAAATCCAG
AACCAACATT
TTCGTGGACT
AAGGATGGCA
ACCCTTTTTA
TTTCACTGAC
CATCGGATAA
TTCCATCGAA
CAATTCAGGA
ACATTCAGGA
TCCCAAACGA
GGGGCACATA
TCTCACTTTC
AAGGGAAATA
CCGCTGCTTT
GCTTCAAATA
AACTGGGAAT
CGCTATGTCA
GAAGAAATAG
AATTTATAGT
TCCAAGTGTT
CCAAAATTCC
CAAAAGAAAA
AATTGACCCT
CTTGAAGTGG
AGGAGGGAGA
TCCAATTGTC
CTCCCATGCA
ATCCTCCCAA
AGGCCTCCCA
CCTTTACACA
TTTATTGGAT
GAATATTGAA
TTAGAACACA
TCGAACAAGA
TGAAAGAGTA
TACATGAGCC
AAAAGGGAGA
TCTATACTTC
GCAAACGTGG
AAGAAAAGGA
CAGTCGCAAT
GACTACTGTT
GCTTTGCTGC
ATTTCCAAGA
TTAAGGACTA
TTGTACAGAA
AATGCCAATG
AAACTAACAG
TTAACAGTTT
AAAGCATGCT
AATGACTCAA
GTTCATCCAC
AGAAATTGGT
TCCAAGGCAA
ATTCCATCAA
GCAAAGAAAA
CCCAAACTGC
TGTTGCCTCC
CACTGAGAGT
GGCAGTGAGT
CTTCAATTAC
CATCCTCAAA
GGGGAAATCT
TGCTGCTTGA
GTGTTTTGCT
GAAGGCTTGC
CAACTCCACA
GGTTGATTGG
AACAAAATTG
GTGGTGACTT
ACCAAAGGGG
AGAGAAACAA
AAGAAAATTA
TGGCAAGACT
TTGAAGATAG
AGAATGTCTC
CTACCAGGAC
AAAGGAAATT
ATCGCTGCAC
AGCCAGCAAT
TTCTTGGGAA
CAGCCACTCA
CGATTTTCAC
GTTATAGTAG
AAGAGCCTCC
TCGCTGGACA
AAGAAGCCTC
AGAGTGCTGT
GTATAGCACC
GGAAGCAATG
GCATCTTGTT
ATGTGAGGCT
GAAGGAGAAC
CTCAACCCAC
AATCAAGTGG
AGAGTCAATG
GCTCCCCAGT
TGACAATCAT
CCATTTGCTG
GTGATGTTGT
CTTCCCCAGG
GAAATCAGTT
TTACCAACCT
TCAACCAAAT
CATACTGCTG
TGTACCAGTG
TGAAGCCTCA
AATGTCCATG
GAACTATCCT
TGCCAATGCC
AATATTGATG
TTGTGGATGT
CCGTCCATTG
ATACAAACCA
AAGATGGAGA
AAATTACGCT
ACAGTGGTTG
GGTACAGTGC
TTTCTTACAT
TGCGAGTTCT
TTGCTTCACC
TGAGGCAGTC
GTGTCCTGGC
AGAAGGTGGA
AGAAGTGAAA
CCCCTGGAGG
GCAGGCGGTA
TCATATCTAT
GAAAATGGCA
CATTGCAGAT
CAACAGAACC
ACCGAAGAAG
ATGCTGGGTC
TTACTCATGT
TGGGTAGAAA
ATGCTATAGG
AAAAACTGCA
GTCACAGCCA
ATTTGGATAT
TAGAAATGCT
ACAAAACTTA
GAGTTTCTCC
TAAGAATCCT
CGTATCCCCA
AATTGCATAT
GCTTGAATTA
CATTGTGAAA
GCAAATGTGA
CTCACATTTG
AAACACAGTT
TGAAGTTGTC
CTGGAGTAAA
GATGGAGAAG
CCTTTGAAAT
TAATGGCACA
GAAGATGGCA
GGATAATTAT
TGATGGAGCT
AATTTGACCA
TATCTAATGT
AACTTTAGAG
GACCAAGGTA
TTTACTGCTG
TTCAGCTCAT
ACTGCTCTAG
ACAGTGCTGC
CGATATAACT
CAAGTAACTG
TTCTTGATGT
TCCGGATCCA
CCAGAAAACC
TTCACTTGTC
TGAAAGACAG
AACAGGAGTG
TTCGGCTGAC
CTGGGAAGCT
GGAGCTGACC
ACAACAGCAA
TATTAGCGAG
TATATTGTTG
AATTTGAAGG
AAACAAAGAA
GAGCCTGGAA
GGTGGGAGGA
ACTGACCAGA
GTCCAAGGAA
AGAAAACCAC
AGTTATCTTA
CCTTTGGCTC
CATTTGTGAG
ATACCAGTTC
AGGGTCATAG
CCGTGAACGA
AGTAGGGAGA
AGTCAGCCTA
GCCAGCCGTC
AGACCATCAT
GAAACACCAC
CAGCAGCTCC
AGATAGGAAT
CCACAAAACA
TAAGGGTTCA
AGCCTCTCAA
CCCAAGGAAA
TGATTATAAA
GTGGGAGCCT
TTGAAATCCA
TGGAGCAGAA
TGGACCAGGC
CTAGAGTACA
GAGTGACCTG
GAAGCCACAG
GGAGCCCCAG
TGGAGTGGGA
AGAAGAAACA
GTCACAAACC
ACACATTGCG
GGTGATGACG
CCTGCTGTCT
ATGCCCCTTA
TGATGTCAAG
GTCCAGGCTA
TCAATCAACT
AGGATCTGGG
CCTGACCCTC
AGTCAGTGAC
TCTCTATTCT
GGAGAAGACT
ATCCTGATAC
AGCTCCAGTG
ATCCATGGGG
TGGACGTTAT
AAACAGTACA
TTAGTTAAAG
TTACCTGGTC
AACAGTTCCA
AAGGACAGAG
TACATGGACG
TCTGAAAGGC
TATCAGATAA
ATTGGTGGAA
AACAAAAAGT
CTGTTGGATG
GAAGAACACA
TCCCAAAGAA
GTGAACATTC
TAAGATTTTC
AGGACAAAGA
AACTCTGGAA
TGGTTCCTTC
CTTAGATGCC
TTTAGTGAAT
TTCATTTAAC
AGTCTTAGCC
TATAACTCTA
AAGGAGCTGG
TCCTGAAAGT
GAGCCTTATA
TATTTCAAAC
ACCAGAAGGA
GTACCTGAAC
AGCCAACTTT
TCTAAAGGTC
ATCAAAGTTG
ATAAAGACAC
TGCCACTTTA
TCTTGGGGAC
TACCTAAGAA
ATTAAATGGA
AACTTAACTG
GCTATCTTTT
GCAATATCAG
ATAATAAATG
ACACCTACGA
GATTGGAGAA
TTAAATGATA
TTAACATTAC
AACTCCATCA
AAGCCCAGCT
GGCACCTCTC
AAACCTGAAT
GCAACTACCA
AGTACAAATT
CTACTTGAGG
GCTTGCACTT
CACAGGGCTG
TGGAAAACCG
ATCACGGAGG
AAAGCTCCAC
CTTAGGAGAA
GGGAGTAAAG
GTATCGGGAA
GATATCAGGA
GTAAATCTTA
CTCAAAAGAC
TCACCCAATA
GAGGTATTTG
AGCCGGGAGC
TGAACATATA
GTTCGCCTAA
TGACTAAGAA
TTGGGGCGAT
AATGATAGCA
TTTTTCAAGA
TGTAATTGAG
ACAAGAGGGA
GAGAATATGC
TGGTTTATAT
GATGACATCT
CCACTCAAGG
CTGGTTTATT
GGACTGATGT
GTGCGATTGC
TCTTCTCACA
CTACTATTAT
TAACTGTTTG
CTTTGTGAAG
AGGAATAGAG
GTGGAAAGTA
CTCAGTTAAA
GAAAAGGAAG
ATTTGCATCC
AGACCCAGAA
ATTCAGTCAG
TAAAAGATGA
AACCTTTGGT
GAATACAGTG
ACAGTGATGA
AAAGCCTCTC
AAAGGAAGCC
TTCGGTCCCT
TAATAGGGAT
ATGCAGCCTA
CTGAAAGTGC
TGACAGCTTA
GTCGAATACG
GAGAGGGAGA
CCATGGTCTC
TTCAGTGAAG
ATGGATCATT
TATTGGTGCC
TACGCTGGAT
CTAAGGAGAA
GGGATCTGTT
GAAAGCAATG
GAAGTTCTAC
AGCAACTTTT
CCCCTTCGGG
CATAAGCGGC
CGCGAAGGAT
CTGCGATCGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGAACGGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGCT
GAAGCTTCGA
GGGGCTCGCA
TCTCTCCTTC
ACGCGCCCGC
CGCCCTACCT
GAGGCCGCCA
TCCACGCCGG
TTGAGTCGCG
TTCTGCCGCC
TCCCGCCTGT
GGTGCCTCCT
GAACTGCGTC
CGCCGTCTAG
GTAAGTTTAA
AGCTCAGGTC
GAGACCGGGC
CTTTGTCCGG
CGCTCCCTTG
GAGCCTACCT
AGACTCAGCC
GGCTCTCCAC
GCTTTGCCTG
ACCCTGCTTG
CTCAACTCTA
CGTCTTTGTT
TCGTTTTCTG
TTCTGCGCCG
TTACAGATCC
AAGCTGTGAC
CGGCGCCTAC
GCTAGATGAC
CGAGTACAAG
CCCACGGTGC
GCCTCGCCAC
CCGCGACGAC
GTCCCCCGGG
CCGTACGCAC
CCTCGCCGCC
GCGTTCGCCG
ACTACCCCGC
CACGCGCCAC
ACCGTCGACC
CGGACCGCCA
CATCGAGCGG
GTCACCGAGC
TGCAAGAACT
CTTCCTCACG
CGCGTCGGGC
TCGACATCGG
CAAGGTGTGG
GTCGCGGACG
ACGGCGCCGC
GGTGGCGGTC
TGGACCACGC
CGGAGAGCGT
CGAAGCGGGG
GCGGTGTTCG
CCGAGATCGG
CCCGCGCATG
GCCGAGTTGA
GCGGTTCCCG
GCTGGCCGCG
CAGCAACAGA
TGGAAGGCCT
CCTGGCGCCG
CACCGGCCCA
AGGAGCCCGC
GTGGTTCCTG
GCCACCGTCG
GCGTCTCGCC
CGACCACCAG
GGCAAGGGTC
TGGGCAGCGC
CGTCGTGCTC
CCCGGAGTGG
AGGCGGCCGA
GCGCGCCGGG
GTGCCCGCCT
TCCTGGAGAC
CTCCGCGCCC
CGCAACCTCC
CCTTCTACGA
GCGGCTCGGC
TTCACCGTCA
CCGCCGACGT
CGAGGTGCCC
GAAGGACCGC
GCACCTGGTG
CATGACCCGC
AAGCCCGGTG
CCTGAGTCGA
CAATCAACCT
CTGGATTACA
AAATTTGTGA
AAGATTGACT
GGTATTCTTA
ACTATGTTGC
TCCTTTTACG
CTATGTGGAT
ACGCTGCTTT
AATGCCTTTG
TATCATGCTA
TTGCTTCCCG
TATGGCTTTC
ATTTTCTCCT
CCTTGTATAA
ATCCTGGTTG
CTGTCTCTTT
ATGAGGAGTT
GTGGCCCGTT
GTCAGGCAAC
GTGGCGTGGT
GTGCACTGTG
TTTGCTGACG
CAACCCCCAC
TGGTTGGGGC
ATTGCCACCA
CCTGTCAGCT
CCTTTCCGGG
ACTTTCGCTT
TCCCCCTCCC
TATTGCCACG
GCGGAACTCA
TCGCCGCCTG
CCTTGCCCGC
TGCTGGACAG
GGGCTCGGCT
GTTGGGCACT
GACAATTCCG
TGGTGTTGTC
GGGGAAATCA
TCGTCCTTTC
CTTGGCTGCT
CGCCTGTGTT
GCCACCTGGA
TTCTGCGCGG
GACGTCCTTC
TGCTACGTCC
CTTCGGCCCT
CAATCCAGCG
GACCTTCCTT
CCCGCGGCCT
GCTGCCGGCT
CTGCGGCCTC
TTCCGCGTCT
TCGCCTTCGC
CCTCAGACGA
GTCGGATCTC
CCTTTGGGCC
GCCTCCCCGC
CTGGTACCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
AATAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
GTAGTTCATG
TCATCTTATT
ATTCAGTATT
TATAACTTGC
AAAGAAATGA
ATATCAGAGA
GTGAGAGGAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
CTCTAGCTAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGACT
TTTGCAGAGA
CGGCCCAAAT
TCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGTCT
AAGAAACCAT
TATTATCATG
ACATTAACCT
ATAAAAATAG
GCGTATCACG
AGGCCCTTTC
GTCTCGCGCG
TTTCGGTGAT
GACGGTGAAA
ACCTCTGACA
CATGCAGCTC
CCGGAGACGG
TCACAGCTTG
TCTGTAAGCG
GATGCCGGGA
GCAGACAAGC
CCGTCAGGGC
GCGTCAGCGG
GTGTTGGCGG
GTGTCGGGGC
TGGCTTAACT
ATGCGGCATC
AGAGCAGATT
GTACTGAGAG
TGCACCATAT
GCGGTGTGAA
ATACCGCACA
GATGCGTAAG
GAGAAAATAC
CGCATCAGGC
GCCATTCGCC
ATTCAGGCTG
CGCAACTGTT
GGGAAGGGCG
ATCGGTGCGG
GCCTCTTCGC
TATTACGCCA
GCTGGCGAAA
GGGGGATGTG
CTGCAAGGCG
ATTAAGTTGG
GTAACGCCAG
GGTTTTCCCA
GTCACGACGT
TGTAAAACGA
CGGCCAGTGC
CAAGCTG
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