产品货号：P63806
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV-Tet-miniCMV-ZNRF3(human-gc-opt)-GFP-nanobody-mCherry-Puro |
|---|---|
| 别称: | NM_001206998.2;BK747E2.3; RNF203 |
| 基因长: | 2808bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达,四环素诱导 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 红色 |
| 启动子: | Tet-miniCMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pLV-Tet-miniCMV-MCS-GFP-nanobody-mCherry-Puro |
| 正向引物: | PFLAG-CMV-F |
| 反向引物: | |
| 诱导方式: | 四环素诱导 |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTTGGAAG
TTTGCAGTGA
ACTCCAAGCC
ACCATGAGAC
CCCGGAGCGG
GGGAAGACCC
GGGGCTACCG
GAAGAAGAAG
AAGAAGGCTG
AGAAGAAGAC
CAAGAGGACT
GAGATGCAGC
AGACTGCCAC
CCCCCCCCCC
CCTGCCTCTG
CTGCTGGGAC
TGCTGCTGGC
TGCCGCTGGA
CCCGGAGCTG
CTAGAGCCAA
AGAAACCGCC
TTCGTGGAGG
TGGTGCTGTT
CGAGAGCAGC
CCCTCCGGCG
ACTACACCAC
CTACACCACC
GGCCTGACCG
GCCGGTTCAG
CAGAGCTGGC
GCCACCCTGA
GCGCCGAGGG
AGAAATTGTG
CAGATGCATC
CCCTGGGCCT
GTGCAACAAC
AACGACGAAG
AGGACCTGTA
CGAATACGGA
TGGGTGGGAG
TGGTGAAGCT
GGAGCAGCCT
GAGCTGGACC
CTAAGCCTTG
CCTGACCGTG
CTGGGCAAAG
CTAAGAGAGC
CGTGCAGAGA
GGAGCCACCG
CTGTGATCTT
TGACGTGAGC
GAGAACCCCG
AGGCCATCGA
CCAGCTGAAC
CAGGGAAGCG
AGGACCCCCT
GAAGAGACCC
GTGGTGTACG
TGAAGGGAGC
CGACGCCATT
AAGCTGATGA
ATATCGTGAA
CAAACAGAAG
GTGGCCAGAG
CCAGGATTCA
GCACAGACCC
CCTAGACAGC
CCACCGAATA
CTTCGACATG
GGAATCTTTC
TGGCCTTCTT
TGTGGTGGTG
AGCCTGGTGT
GCCTGATCCT
GCTGGTGAAG
ATTAAGCTGA
AGCAGAGAAG
AAGCCAGAAC
AGCATGAACA
GACTGGCCGT
GCAGGCCCTG
GAGAAGATGG
AGACCAGGAA
GTTCAATAGC
AAAAGCAAGG
GAAGGAGAGA
AGGAAGCTGC
GGAGCCCTGG
ACACCCTGAG
CAGCAGCTCC
ACCAGCGACT
GCGCCATTTG
CCTGGAAAAG
TACATCGACG
GCGAGGAGCT
GAGGGTGATC
CCATGCACAC
ACAGATTTCA
TAGAAAGTGC
GTGGACCCCT
GGCTGCTGCA
GCACCACACC
TGCCCCCACT
GTAGACACAA
CATCATCGAG
CAGAAAGGCA
ACCCCTCCGC
CGTGTGCGTG
GAGACCAGCA
ACCTGTCCAG
AGGCCGGCAG
CAGAGAGTGA
CCCTGCCCGT
GCACTACCCC
GGCAGAGTGC
ACAGAACCAA
CGCCATCCCC
GCCTACCCCA
CCCGCACCTC
TATGGATAGC
CACGGCAACC
CCGTGACCCT
GCTGACCATG
GACAGACACG
GCGAGCAGAG
CCTGTACAGC
CCCCAGACCC
CCGCCTACAT
CAGAAGCTAC
CCCCCCCTGC
ACCTGGACCA
CAGCCTGGCT
GCTCACAGAT
GTGGCCTGGA
ACACAGAGCC
TACAGCCCTG
CCCACCCCTT
CCGCAGACCC
AAACTGTCCG
GACGCAGCTT
CTCCAAGGCC
GCCTGCTTCT
CTCAGTACGA
GACTATGTAT
CAGCACTACT
ATTTCCAGGG
CCTGTCCTAC
CCCGAACAGG
AGGGCCAGAG
CCCCCCCTCT
CTGGCTCCTA
GAGGACCTGC
CCGCGCCTTC
CCCCCTTCCG
GATCTGGATC
CCTGCTGTTC
CCCACCGTGG
TGCACGTGGC
CCCCCCTTCT
CACCTGGAGT
CTGGCAGCAC
CTCCTCCTTC
TCCTGCTACC
ATGGCCACAG
ATCCGTGTGC
TCCGGCTACC
TGGCTGACTG
CCCCGGCAGC
GACAGCTCTT
CCTCCTCCTC
CTCTGGCCAG
TGCCACTGCA
GCTCCTCCGA
TTCCGTGGTG
GACTGCACCG
AAGTGTCCAA
CCAGGGGGTG
TATGGCAGCT
GCAGCACCTT
CAGAAGCAGC
CTGTCCTCCG
ACTATGACCC
CTTTATCTAC
AGAAGCAGAA
GCCCCTGCCG
CGCTAGCGAA
GCCGGCGGAA
GTGGCAGCTC
AGGCAGAGGC
CCCGCCCTGT
GTTTCGAGGG
CAGCCCACCC
CCCGAGGAGC
TGCCTGCTGT
GCACAGCCAC
GGAGCCGGAA
GAGGCGAGCC
TTGGCCAGGA
CCCGCTAGCC
CTTCCGGCGA
CCAGGTGAGC
ACCTGCAGCC
TGGAGATGAA
CTACAGCAGC
AACAGCTCCC
TGGAGCATAG
AGGACCCAAT
TCTAGTACAT
CCGAGGTGGG
GCTGGAAGCC
TCTCCCGGCG
CTGCTCCTGA
CCTGAGAAGA
ACCTGGAAAG
GAGGGCACGA
GCTGCCCAGC
TGCGCTTGCT
GTTGCGAGCC
CCAGCCCTCC
CCCGCTGGAC
CTTCTGCTGG
CGCTGCCGGA
TCCAGCACCC
TGTTCCTGGG
CCCCCACCTG
TACGAGGGAA
GCGGCCCTGC
CGGCGGAGAG
CCTCAGTCTG
GAAGCTCACA
GGGCCTGTAC
GGACTGCACC
CTGATCACCT
GCCCAGAACC
GACGGCGTGA
AGTACGAGGG
CCTGCCCTGC
TGTTTTTATG
AGGAAAAGCA
GGTGGCTAGA
GGCGGGGGGG
GAGGCTCAGG
ATGCTACACC
GAAGACTACA
GCGTGAGCGT
GCAGTACACA
CTGACCGAGG
AGCCACCTCC
AGGCTGTTAT
CCCGGCGCCA
GAGACCTGTC
TCAGAGAATC
CCTATTATCC
CTGAGGACGT
GGACTGCGAC
CTGGGCCTGC
CTTCCGATTG
CCAGGGCACC
CACAGCCTGG
GCTCCTGGGG
AGGAACCAGA
GGCCCCGACA
CACCCAGGCC
ACACCGGGGA
CTGGGCGCTA
CAAGAGAGGA
AGAGAGAGCT
CTGTGCTGCC
AGGCCAGAGC
CCTGCTGAGA
CCCGGCTGCC
CTCCCGAAGA
AGCCGGAGCT
GTGAGAGCCA
ACTTCCCCAG
CGCTCTGCAG
GATACCCAGG
AAAGCTCAAC
CACAGCCACC
GAGGCCGCCG
GACCCAGATC
CCATTCCGCC
GACTCCTCCA
GCCCAGGAGC
CTACCCCTAC
GACGTGCCTG
ACTACGCCGG
CGGCGGTGGT
TCAGGCGGTG
GCGGTTCCGG
TGGTGGGGGA
TCAGTCCAAC
TGGTGGAGTC
TGGTGGCGCT
TTGGTGCAGC
CAGGTGGCTC
TCTGCGTTTG
TCCTGTGCCG
CTTCTGGCTT
CCCAGTGAAC
CGCTATTCCA
TGCGCTGGTA
TCGCCAGGCT
CCAGGCAAAG
AGCGTGAGTG
GGTAGCCGGT
ATGTCCAGCG
CGGGTGATCG
TAGCTCCTAT
GAAGACTCCG
TGAAGGGCCG
TTTCACCATC
AGCCGTGACG
ATGCCCGTAA
CACGGTGTAT
CTGCAAATGA
ACAGCTTGAA
ACCTGAAGAT
ACGGCCGTGT
ATTACTGTAA
TGTGAACGTG
GGCTTCGAGT
ATTGGGGCCA
AGGCACCCAG
GTCACCGTCT
CCAGCTAAGA
TCCTTGACTT
GCGGCCGCAA
CTCCCACCTG
CAACATGCGT
GACTGACTGA
GGCCGCGACT
CTAGAGTCGA
CCTGCATCTA
GGCGCCGGAA
TTAGATCTCT
CGAGGTTAAC
GAATTCTACC
GGGTAGGGGA
GGCGCTTTTC
CCAAGGCAGT
CTGGAGCATG
CGCTTTAGCA
GCCCCGCTGG
GCACTTGGCG
CTACACAAGT
GGCCTCTGGC
CTCGCACACA
TTCCACATCC
ACCGGTAGGC
GCCAACCGGC
TCCGTTCTTT
GGTGGCCCCT
TCGCGCCACC
TTCTACTCCT
CCCCTAGTCA
GGAAGTTCCC
CCCCGCCCCG
CAGCTCGCGT
CGTGCAGGAC
GTGACAAATG
GAAGTAGCAC
GTCTCACTAG
TCTCGTGCAG
ATGGACAGCA
CCGCTGAGCA
ATGGAAGCGG
GTAGGCCTTT
GGGGCAGCGG
CCAATAGCAG
CTTTGCTCCT
TCGCTTTCTG
GGCTCAGAGG
CTGGGAAGGG
GTGGGTCCGG
GGGCGGGCTC
AGGGGCGGGC
TCAGGGGCGG
GGCGGGCGCC
CGAAGGTCCT
CCGGAGGCCC
GGCATTCTGC
ACGCTTCAAA
AGCGCACGTC
TGCCGCGCTG
TTCTCCTCTT
CCTCATCTCC
GGGCCTTTCG
ACCTGCAGCC
CAAGCTTACC
ATGGTGAGCA
AGGGCGAGGA
GGATAACATG
GCCATCATCA
AGGAGTTCAT
GCGCTTCAAG
GTGCACATGG
AGGGCTCCGT
GAACGGCCAC
GAGTTCGAGA
TCGAGGGCGA
GGGCGAGGGC
CGCCCCTACG
AGGGCACCCA
GACCGCCAAG
CTGAAGGTGA
CCAAGGGTGG
CCCCCTGCCC
TTCGCCTGGG
ACATCCTGTC
CCCTCAGTTC
ATGTACGGCT
CCAAGGCCTA
CGTGAAGCAC
CCCGCCGACA
TCCCCGACTA
CTTGAAGCTG
TCCTTCCCCG
AGGGCTTCAA
GTGGGAGCGC
GTGATGAACT
TCGAGGACGG
CGGCGTGGTG
ACCGTGACCC
AGGACTCCTC
CCTGCAGGAC
GGCGAGTTCA
TCTACAAGGT
GAAGCTGCGC
GGCACCAACT
TCCCCTCCGA
CGGCCCCGTA
ATGCAGAAGA
AGACCATGGG
CTGGGAGGCC
TCCTCCGAGC
GGATGTACCC
CGAGGACGGC
GCCCTGAAGG
GCGAGATCAA
GCAGAGGCTG
AAGCTGAAGG
ACGGCGGCCA
CTACGACGCT
GAGGTCAAGA
CCACCTACAA
GGCCAAGAAG
CCCGTGCAGC
TGCCCGGCGC
CTACAACGTC
AACATCAAGT
TGGACATCAC
CTCCCACAAC
GAGGACTACA
CCATCGTGGA
ACAGTACGAA
CGCGCCGAGG
GCCGCCACTC
CACCGGCGGC
ATGGACGAGC
TGTACAAGGC
AACAAACTTC
TCTCTGCTGA
AACAAGCCGG
AGATGTCGAA
GAGAATCCTG
GACCGATGAC
CGAGTACAAG
CCCACGGTGC
GCCTCGCCAC
CCGCGACGAC
GTCCCCAGGG
CCGTACGCAC
CCTCGCCGCC
GCGTTCGCCG
ACTACCCCGC
CACGCGCCAC
ACCGTCGATC
CGGACCGCCA
CATCGAGCGG
GTCACCGAGC
TGCAAGAACT
CTTCCTCACG
CGCGTCGGGC
TCGACATCGG
CAAGGTGTGG
GTCGCGGACG
ACGGCGCCGC
GGTGGCGGTC
TGGACCACGC
CGGAGAGCGT
CGAAGCGGGG
GCGGTGTTCG
CCGAGATCGG
CCCGCGCATG
GCCGAGTTGA
GCGGTTCCCG
GCTGGCCGCG
CAGCAACAGA
TGGAAGGCCT
CCTGGCGCCG
CACCGGCCCA
AGGAGCCCGC
GTGGTTCCTG
GCCACCGTCG
GCGTCTCGCC
CGACCACCAG
GGCAAGGGTC
TGGGCAGCGC
CGTCGTGCTC
CCCGGAGTGG
AGGCGGCCGA
GCGCGCCGGG
GTGCCCGCCT
TCCTGGAGAC
CTCCGCGCCC
CGCAACCTCC
CCTTCTACGA
GCGGCTCGGC
TTCACCGTCA
CCGCCGACGT
CGAGGTGCCC
GAAGGACCGC
GCACCTGGTG
CATGACCCGC
AAGCCCGGTG
CCTGATCTTA
TCGATAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCATCGAT
ACCGTCGACC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAGACAAGA
TCTGCTTTTT
GCTTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GCATCTAGAA
TTAATTCCGT
GTATTCTATA
GTGTCACCTA
AATCGTATGT
GTATGATACA
TAAGGTTATG
TATTAATTGT
AGCCGCGTTC
TAACGACAAT
ATGTACAAGC
CTAATTGTGT
AGCATCTGGC
TTACTGAAGC
AGACCCTATC
ATCTCTCTCG
TAAACTGCCG
TCAGAGTCGG
TTTGGTTGGA
CGAACCTTCT
GAGTTTCTGG
TAACGCCGTC
CCGCACCCGG
AAATGGTCAG
CGAACCAATC
AGCAGGGTCA
TCGCTAGCCA
GATCCTCTAC
GCCGGACGCA
TCGTGGCCGG
CATCACCGGC
GCCACAGGTG
CGGTTGCTGG
CGCCTATATC
GCCGACATCA
CCGATGGGGA
AGATCGGGCT
CGCCACTTCG
GGCTCATGAG
CGCTTGTTTC
GGCGTGGGTA
TGGTGGCAGG
CCCCGTGGCC
GGGGGACTGT
TGGGCGCCAT
CTCCTTGCAT
GCACCATTCC
TTGCGGCGGC
GGTGCTCAAC
GGCCTCAACC
TACTACTGGG
CTGCTTCCTA
ATGCAGGAGT
CGCATAAGGG
AGAGCGTCGA
ATGGTGCACT
CTCAGTACAA
TCTAGCTCTG
ATGCCGCATA
GTTAAGCCAG
CCCCGACACC
CGCCAACACC
CGCTGACGCG
CCCTGACGGG
CTTGTCTGCT
CCCGGCATCC
GCTTACAGAC
AAGCTGTGAC
CGTCTCCGGG
AGCTGCATGT
GTCAGAGGTT
TTCACCGTCA
TCACCGAAAC
GCGCGAGACG
AAAGGGCCTC
GTGATACGCC
TATTTTTATA
GGTTAATGTC
ATGATAATAA
TGGTTTCTTA
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTCCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAGAGC
GCCCAATACG
CAAACCGCCT
CTCCCCGCGC
GTTGGCCGAT
TCATTAATGC
AGCTGTGGAA
TGTGTGTCAG
TTAGGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCAGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCATA
GTCCCGCCCC
TAACTCCGCC
CATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
GGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTTGGA
CACAAGACAG
GCTTGCGAGA
TATGTTTGAG
AATACCACTT
TATCCCGCGT
CAGGGAGAGG
CAGTGCGTAA
AAAGACGCGG
ACTCATGTGA
AATACTGGTT
TTTAGTGCGC
CAGATCTCTA
TAATCTCGCG
CAACCTATTT
TCCCCTCGAA
CACTTTTTAA
GCCGTAGATA
AACAGGCTGG
GACACTTCAC
ATGAGCGAAA
AATACATCGT
CACCTGGGAC
ATGTTGCAGA
TCCATGCACG
TAAACTCGCA
AGCCGACTGA
TGCCTTCTGA
ACAATGGAAA
GGCATTATTG
CCGTAAGCCG
TGGCGGTCTG
TACCGGGTGC
GTTACTGGCG
CGTGAACTGG
GTATTCGTCA
TGTCGATACC
GTTTGTATTT
CCAGCTACGA
TCACGACAAC
CAGCGCGAGC
TTAAAGTGCT
GAAACGCGCA
GAAGGCGATG
GCGAAGGCTT
CATCGTTATT
GATGACCTGG
TGGATACCGG
TGGTACTGCG
GTTGCGATTC
GTGAAATGTA
TCCAAAAGCG
CACTTTGTCA
CCATCTTCGC
AAAACCGGCT
GGTCGTCCGC
TGGTTGATGA
CTATGTTGTT
GATATCCCGC
AAGATACCTG
GATTGAACAG
CCGTGGGATA
TGGGCGTCGT
ATTCGTCCCG
CCAATCTCCG
GTCGCTAATC
TTTTCAACGC
CTGGCACTGC
CGGGCGTTGT
TCTTTTTAAC
TTCAGGCGGG
TTACAATAGT
TTCCAGTAAG
TATTCTGGAG
GCTGCATCCA
TGACACAGGC
AAACCTGAGC
GAAACCCTGT
TCAAACCCCG
CTTTAAACAT
CCTGAAACCT
CGACGCTAGT
CCGCCGCTTT
AATCACGGCG
CACAACCGCC
TGTGCAGTCG
GCCCTTGATG
GTAAAACCAT
CCCTCACTGG
TATCGCATGA
TTAACCGTCT
GATGTGGATC
TGGCGCGGCA
TTGACCCACG
CGAAATCCTC
GACGTCCAGG
CACGTATTGT
GATGAGCGAT
GCCGAACGTA
CCGACGATGA
TTTATACGAT
ACGGTGATTG
GCTACCGTGG
CGGCAACTGG
ATTTATGAGT
GGGCCCCGGA
TCTTTGTGAA
GGAACCTTAC
TTCTGTGGTG
TGACATAATT
GGACAAACTA
CCTACAGAGA
TTTAAAGCTC
TAAGGTAAAT
ATAAAATTTT
TAAGTGTATA
ATGTGTTAAA
CTACTGATTC
TAATTGTTTG
TGTATTTTAG
ATTCCAACCT
ATGGAACTGA
TGAATGGGAG
CAGTGGTGGA
ATGCCTTTAA
TGAGGAAAAC
CTGTTTTGCT
CAGAAGAAAT
GCCATCTAGT
GATGATGAGG
CTACTGCTGA
CTCTCAACAT
TCTACTCCTC
CAAAAAAGAA
GAGAAAGGTA
GAAGACCCCA
AGGACTTTCC
TTCAGAATTG
CTAAGTTTTT
TGAGTCATGC
TGTGTTTAGT
AATAGAACTC
TTGCTTGCTT
TGCTATTTAC
ACCACAAAGG
AAAAAGCTGC
ACTGCTATAC
AAGAAAATTA
TGGAAAAATA
TTCTGTAACC
TTTATAAGTA
GGCATAACAG
TTATAATCAT
AACATACTGT
TTTTTCTTAC
TCCACACAGG
CATAGAGTGT
CTGCTATTAA
TAACTATGCT
CAAAAATTGT
GTACCTTTAG
CTTTTTAATT
TGTAAAGGGG
TTAATAAGGA
ATATTTGATG
TATAGTGCCT
TGACTAGAGA
TCATAATCAG
CCATACCACA
TTTGTAGAGG
TTTTACTTGC
TTTAAAAAAC
CTCCCACACC
TCCCCCTGAA
CCTGAAACAT
AAAATGAATG
CAATTGTTGT
TGTTAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGATCA
ACTGGATAAC
TCAAGCTAAC
CAAAATCATC
CCAAACTTCC
CACCCCATAC
CCTATTACCA
CTGCCAATTA
CCTAGTGGTT
TCATTTACTC
TAAACCTGTG
ATTCCTCTGA
ATTATTTTCA
TTTTAAAGAA
ATTGTATTTG
TTAAATATGT
ACTACAAACT
TAGTAGTTGG
AAGGGCTAAT
TCACTCCCAA
AGAAGACAAG
ATATCCTTGA
TCTGTGGATC
TACCACACAC
AAGGCTACTT
CCCTGATTAG
CAGAACTACA
CACCAGGGCC
AGGGGTCAGA
TATCCACTGA
CCTTTGGATG
GTGCTACAAG
CTAGTACCAG
TTGAGCCAGA
TAAGGTAGAA
GAGGCCAATA
AAGGAGAGAA
CACCAGCTTG
TTACACCCTG
TGAGCCTGCA
TGGGATGGAT
GACCCGGAGA
GAGAAGTGTT
AGAGTGGAGG
TTTGACAGCC
GCCTAGCATT
TCATCACGTG
GCCCGAGAGC
TGCATCCGGA
GTACTTCAAG
AACTGCTGAT
ATCGAGCTTG
CTACAAGGGA
CTTTCCGCTG
GGGACTTTCC
AGGGAGGCGT
GGCCTGGGCG
GGACTGGGGA
GTGGCGAGCC
CTCAGATCCT
GCATATAAGC
AGCTGCTTTT
TGCCTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTGGCGCCC
GAACAGGGAC
TTGAAAGCGA
AAGGGAAACC
AGAGGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCGGTGA
TCTTCAGACC
TGGACGATAT
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTCG
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