名称: | pHAGE-TO-GAG-2-nmdCas9-3XmCherry |
---|---|
别称: | · |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 红色 |
启动子: | CMV/TO |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pHAGE-TO-nmdCas9-3XmCherry |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTCT
ATATAAGCAG
AGCTCTCCCT
ATCAGTGATA
GAGATCTCCC
TATCAGTGAT
AGAGATCGTC
GACGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGGGAC
CGATCCAGCC
TACCGGTTAA
GGTGAACGCG
TGCAGGCTGG
CGCCACCATG
GCCATGTTCA
TACCTCTTAT
CTTCCTCCCA
CAGCTCCTGG
GCAACGTGCT
GGTCTGTGTG
CTGGCCCATC
ACTTTGGCAA
AGAATTCATG
GGCCAGACTG
TTACCACTCC
CTTAAGTTTG
ACCTTAGGTC
ACTGGAAAGA
TGTCGAGCGG
ATCGCTCACA
ACCAGTCGGT
AGATGTCAAG
AAGAGACGTT
GGGTTACCTT
CTGCTCTGCA
GAATGGCCAA
CCTTTAACGT
CGGATGGCCG
CGAGACGGCA
CCTTTAACCG
AGACCTCATC
ACCCAGGTTA
AGATCAAGGT
CTTTTCACCT
GGCCCGCATG
GACACCCAGA
CCAGGTCCCC
TACATCGTGA
CCTGGGAAGC
CTTGGCTTTT
GACCCCCCTC
CCTGGGTCAA
GCCCTTTGTA
CACCCTAAGC
CTCCGCCTCC
TCTTCCTCCA
TCCGCCCCGT
CTCTCCCCCT
TGAACCTCCT
CGTTCGACCC
CGCCTCGATC
CTCCCTTTAT
CCAGCCCTCA
CTCCTTCTCT
AGGCGCCAAA
CCTAAACCTC
AAGTTCTTTC
TGACAGTGGG
GGGCCGCTCA
TCGACCTACT
TACAGAAGAC
CCCCCGCCTT
ATAGGGACCC
AAGACCACCC
CCTTCCGACA
GGGACGGAAA
TGGTGGAGAA
GCGACCCCTG
CGGGAGAGGC
ACCGGACCCC
TCCCCAATGG
CATCTCGCCT
ACGTGGGAGA
CGGGAGCCCC
CTGTGGCCGA
CTCCACTACC
TCGCAGGCAT
TCCCCCTCCG
CGCAGGAGGA
AACGGACAGC
TTCAATACTG
GCCGTTCTCC
TCTTCTGACC
TTTACAACTG
GAAAAATAAT
AACCCTTCTT
TTTCTGAAGA
TCCAGGTAAA
CTGACAGCTC
TGATCGAGTC
TGTTCTCATC
ACCCATCAGC
CCACCTGGGA
CGACTGTCAG
CAGCTGTTGG
GGACTCTGCT
GACCGGAGAA
GAAAAACAAC
GGGTGCTCTT
AGAGGCTAGA
AAGGCGGTGC
GGGGCGATGA
TGGGCGCCCC
ACTCAACTGC
CCAATGAAGT
CGATGCCGCT
TTTCCCCTCG
AGCGCCCAGA
CTGGGATTAC
ACCACCCAGG
CAGGTAGGAA
CCACCTAGTC
CACTATCGCC
AGTTGCTCCT
AGCGGGTCTC
CAAAACGCGG
GCAGAAGCCC
CACCAATTTG
GCCAAGGTAA
AAGGAATAAC
ACAAGGGCCC
AATGAGTCTC
CCTCGGCCTT
CCTAGAGAGA
CTTAAGGAAG
CCTATCGCAG
GTACACTCCT
TATGACCCTG
AGGACCCAGG
GCAAGAAACT
AATGTGTCTA
TGTCTTTCAT
TTGGCAGTCT
GCCCCAGACA
TTGGGAGAAA
GTTAGAGAGG
TTAGAAGATT
TAAAAAACAA
GACGCTTGGA
GATTTGGTTA
GAGAGGCAGA
AAAGATCTTT
AATAAACGAG
AAACCCCGGA
AGAAAGAGAG
GAACGTATCA
GGAGAGAAAC
AGAGGAAAAA
GAAGAACGCC
GTAGGACAGA
GGATGAGCAG
AAAGAGAAAG
AAAGAGATCG
TAGGAGACAT
AGAGAGATGA
GCAAGCTATT
GGCCACTGTC
GTTAGTGGAC
AGAAACAGGA
TAGACAGGGA
GGAGAACGAA
GGAGGTCCCA
ACTCGATCGC
GACCAGTGTG
CCTACTGCAA
AGAAAAGGGG
CACTGGGCTA
AAGATTGTCC
CAAGAAACCA
CGAGGACCTC
GGGGACCAAG
ACCGCAACCG
GTCGCCACCC
CTAAAAAGAA
ACGCAAGGTC
GAATTCGGAA
GCGCCGCCTT
CAAGCCCAAC
CCCATCAACT
ACATCCTGGG
CCTGGCCATC
GGCATCGCCA
GCGTGGGCTG
GGCCATGGTG
GAGATCGACG
AGGACGAGAA
CCCCATCTGC
CTGATCGACC
TGGGTGTGCG
CGTGTTCGAG
CGCGCTGAGG
TGCCCAAGAC
TGGTGACAGT
CTGGCTATGG
CTCGCCGGCT
TGCTCGCTCT
GTTCGGCGCC
TTACTCGCCG
GCGCGCTCAC
CGCCTTCTGC
GCGCTCGCCG
CCTGCTGAAG
CGCGAGGGTG
TGCTGCAGGC
TGCCGACTTC
GACGAGAACG
GCCTGATCAA
GAGCCTGCCC
AACACTCCTT
GGCAGCTGCG
CGCTGCCGCT
CTGGACCGCA
AGCTGACTCC
TCTGGAGTGG
AGCGCCGTGC
TGCTGCACCT
GATCAAGCAC
CGCGGCTACC
TGAGCCAGCG
CAAGAACGAG
GGCGAGACCG
CCGACAAGGA
GCTGGGTGCT
CTGCTGAAGG
GCGTGGCCGA
CAACGCCCAC
GCCCTGCAGA
CTGGTGACTT
CCGCACTCCT
GCTGAGCTGG
CCCTGAACAA
GTTCGAGAAG
GAGAGCGGCC
ACATCCGCAA
CCAGCGCGGC
GACTACAGCC
ACACCTTCAG
CCGCAAGGAC
CTGCAGGCCG
AGCTGATCCT
GCTGTTCGAG
AAGCAGAAGG
AGTTCGGCAA
CCCCCACGTG
AGCGGCGGCC
TGAAGGAGGG
CATCGAGACC
CTGCTGATGA
CCCAGCGCCC
CGCCCTGAGC
GGCGACGCCG
TGCAGAAGAT
GCTGGGCCAC
TGCACCTTCG
AGCCAGCCGA
GCCCAAGGCC
GCCAAGAACA
CCTACACCGC
CGAGCGCTTC
ATCTGGCTGA
CCAAGCTGAA
CAACCTGCGC
ATCCTGGAGC
AGGGCAGCGA
GCGCCCCCTG
ACCGACACCG
AGCGCGCCAC
CCTGATGGAC
GAGCCCTACC
GCAAGAGCAA
GCTGACCTAC
GCCCAGGCCC
GCAAGCTGCT
GGGTCTGGAG
GACACCGCCT
TCTTCAAGGG
CCTGCGCTAC
GGCAAGGACA
ACGCCGAGGC
CAGCACCCTG
ATGGAGATGA
AGGCCTACCA
CGCCATCAGC
CGCGCCCTGG
AGAAGGAGGG
CCTGAAGGAC
AAGAAGAGTC
CTCTGAACCT
GAGCCCCGAG
CTGCAGGACG
AGATCGGCAC
CGCCTTCAGC
CTGTTCAAGA
CCGACGAGGA
CATCACCGGC
CGCCTGAAGG
ACCGCATCCA
GCCCGAGATC
CTGGAGGCCC
TGCTGAAGCA
CATCAGCTTC
GACAAGTTCG
TGCAGATCAG
CCTGAAGGCC
CTGCGCCGCA
TCGTGCCCCT
GATGGAGCAG
GGCAAGCGCT
ACGACGAGGC
CTGCGCCGAG
ATCTACGGCG
ACCACTACGG
CAAGAAGAAC
ACCGAGGAGA
AGATCTACCT
GCCTCCTATC
CCCGCCGACG
AGATCCGCAA
CCCCGTGGTG
CTGCGCGCCC
TGAGCCAGGC
CCGCAAGGTG
ATCAACGGCG
TGGTGCGCCG
CTACGGCAGC
CCCGCCCGCA
TCCACATCGA
GACCGCCCGC
GAGGTGGGCA
AGAGCTTCAA
GGACCGCAAG
GAGATCGAGA
AGCGCCAGGA
GGAGAACCGC
AAGGACCGCG
AGAAGGCCGC
CGCCAAGTTC
CGCGAGTACT
TCCCCAACTT
CGTGGGCGAG
CCCAAGAGCA
AGGACATCCT
GAAGCTGCGC
CTGTACGAGC
AGCAGCACGG
CAAGTGCCTG
TACAGCGGCA
AGGAGATCAA
CCTGGGCCGC
CTGAACGAGA
AGGGCTACGT
GGAGATCGCC
GCTGCCCTGC
CCTTCAGCCG
CACCTGGGAC
GACAGCTTCA
ACAACAAGGT
GCTGGTGCTG
GGCAGCGAGG
CTCAGAACAA
GGGCAACCAG
ACCCCCTACG
AGTACTTCAA
CGGCAAGGAC
AACAGCCGCG
AGTGGCAGGA
GTTCAAGGCC
CGCGTGGAGA
CCAGCCGCTT
CCCCCGCAGC
AAGAAGCAGC
GCATCCTGCT
GCAGAAGTTC
GACGAGGACG
GCTTCAAGGA
GCGCAACCTG
AACGACACCC
GCTACGTGAA
CCGCTTCCTG
TGCCAGTTCG
TGGCCGACCG
CATGCGCCTG
ACCGGCAAGG
GCAAGAAGCG
CGTGTTCGCC
AGCAACGGCC
AGATCACCAA
CCTGCTGCGC
GGCTTCTGGG
GCCTGCGCAA
GGTGCGCGCC
GAGAACGACC
GCCACCACGC
CCTGGACGCC
GTGGTGGTGG
CCTGCAGCAC
CGTGGCCATG
CAGCAGAAGA
TCACCCGCTT
CGTGCGCTAC
AAGGAGATGA
ACGCCTTCGA
CGGTAAAACC
ATCGACAAGG
AGACCGGCGA
GGTGCTGCAC
CAGAAGACCC
ACTTCCCCCA
GCCCTGGGAG
TTCTTCGCCC
AGGAGGTGAT
GATCCGCGTG
TTCGGCAAGC
CCGACGGCAA
GCCCGAGTTC
GAGGAGGCCG
ACACCCCCGA
GAAGCTGCGC
ACCCTGCTGG
CCGAGAAGCT
GAGCAGCCGC
CCTGAGGCCG
TGCACGAGTA
CGTGACTCCT
CTGTTCGTGA
GCCGCGCCCC
CAACCGCAAG
ATGAGCGGTC
AGGGTCACAT
GGAGACCGTG
AAGAGCGCCA
AGCGCCTGGA
CGAGGGCGTG
AGCGTGCTGC
GCGTGCCCCT
GACCCAGCTG
AAGCTGAAGG
ACCTGGAGAA
GATGGTGAAC
CGCGAGCGCG
AGCCCAAGCT
GTACGAGGCC
CTGAAGGCCC
GCCTGGAGGC
CCACAAGGAC
GACCCCGCCA
AGGCCTTCGC
CGAGCCCTTC
TACAAGTACG
ACAAGGCCGG
CAACCGCACC
CAGCAGGTGA
AGGCCGTGCG
CGTGGAGCAG
GTGCAGAAGA
CCGGCGTGTG
GGTGCGCAAC
CACAACGGCA
TCGCCGACAA
CGCCACCATG
GTGCGCGTGG
ACGTGTTCGA
GAAGGGCGAC
AAGTACTACC
TGGTGCCCAT
CTACAGCTGG
CAGGTGGCCA
AGGGCATCCT
GCCCGACCGC
GCCGTGGTGC
AGGGCAAGGA
CGAGGAGGAC
TGGCAGCTGA
TCGACGACAG
CTTCAACTTC
AAGTTCAGCC
TGCACCCCAA
CGACCTGGTG
GAGGTGATCA
CCAAGAAGGC
CCGCATGTTC
GGCTACTTCG
CCAGCTGCCA
CCGCGGCACC
GGCAACATCA
ACATCCGCAT
CCACGACCTG
GACCACAAGA
TCGGCAAGAA
CGGCATCCTG
GAGGGCATCG
GCGTGAAGAC
CGCCCTGAGC
TTCCAGAAGT
ACCAGATCGA
CGAGCTGGGC
AAGGAGATCC
GCCCCTGCCG
CCTGAAGAAG
CGCCCTCCTG
TGCGCGGCTC
TACTAGTGGC
TCTCCCAAGA
AGAAGAGGAA
GGTAGGATCC
GGAAGTATGG
TGAGCAAGGG
CGAGGAGGAT
AACATGGCCA
TCATCAAGGA
GTTCATGCGC
TTCAAGGTGC
ACATGGAGGG
CTCCGTGAAC
GGCCACGAGT
TCGAGATCGA
GGGCGAGGGC
GAGGGCCGCC
CCTACGAGGG
CACCCAGACC
GCCAAGCTGA
AGGTGACCAA
GGGTGGCCCC
CTGCCCTTCG
CCTGGGACAT
CCTGTCCCCT
CAGTTCATGT
ACGGCTCCAA
GGCCTACGTG
AAGCACCCCG
CCGACATCCC
CGACTACTTG
AAGCTGTCCT
TCCCCGAGGG
CTTCAAGTGG
GAGCGCGTGA
TGAACTTCGA
GGACGGCGGC
GTGGTGACCG
TGACCCAGGA
CTCCTCACTG
CAGGACGGCG
AGTTCATCTA
CAAGGTGAAG
CTGCGCGGCA
CCAACTTCCC
CTCCGACGGC
CCCGTAATGC
AGAAGAAGAC
CATGGGCTGG
GAGGCCTCCT
CCGAGCGGAT
GTACCCCGAG
GACGGCGCCC
TGAAGGGCGA
GATCAAGCAG
AGGCTGAAGC
TGAAGGACGG
CGGCCACTAC
GACGCTGAGG
TCAAGACCAC
CTACAAGGCC
AAGAAGCCCG
TGCAGCTGCC
CGGCGCCTAC
AACGTCAACA
TCAAGTTGGA
CATCACCTCC
CACAACGAGG
ACTACACCAT
CGTGGAACAG
TACGAACGCG
CTGAGGGCCG
CCACTCCACC
GGCGGCATGG
ACGAGCTGTA
CAAGGGTAGC
ATCGATGGTA
GCATGGTGAG
CAAGGGCGAG
GAGGATAACA
TGGCCATCAT
CAAGGAGTTC
ATGCGCTTCA
AGGTGCACAT
GGAGGGCTCC
GTGAACGGCC
ACGAGTTCGA
GATCGAGGGC
GAGGGCGAGG
GCCGCCCCTA
CGAGGGCACC
CAGACCGCCA
AGCTGAAGGT
GACCAAGGGT
GGCCCCCTGC
CCTTCGCCTG
GGACATCCTG
TCCCCTCAGT
TCATGTACGG
CTCCAAGGCC
TACGTGAAGC
ACCCCGCCGA
CATCCCCGAC
TACTTGAAGC
TGTCCTTCCC
CGAGGGCTTC
AAGTGGGAGC
GCGTGATGAA
CTTCGAGGAC
GGCGGCGTGG
TGACCGTGAC
CCAGGACTCC
TCACTGCAGG
ACGGCGAGTT
CATCTACAAG
GTGAAGCTGC
GCGGCACCAA
CTTCCCCTCC
GACGGCCCCG
TAATGCAGAA
GAAGACCATG
GGCTGGGAGG
CCTCCTCCGA
GCGGATGTAC
CCCGAGGACG
GCGCCCTGAA
GGGCGAGATC
AAGCAGAGGC
TGAAGCTGAA
GGACGGCGGC
CACTACGACG
CTGAGGTCAA
GACCACCTAC
AAGGCCAAGA
AGCCCGTGCA
GCTGCCCGGC
GCCTACAACG
TCAACATCAA
GTTGGACATC
ACCTCCCACA
ACGAGGACTA
CACCATCGTG
GAACAGTACG
AACGCGCTGA
GGGCCGCCAC
TCCACCGGCG
GCATGGACGA
GCTGTACAAG
TCTGGCGGCC
GCTCTGGCAT
GGTGAGCAAG
GGCGAGGAGG
ATAACATGGC
CATCATCAAG
GAGTTCATGC
GCTTCAAGGT
GCACATGGAG
GGCTCCGTGA
ACGGCCACGA
GTTCGAGATC
GAGGGCGAGG
GCGAGGGCCG
CCCCTACGAG
GGCACCCAGA
CCGCCAAGCT
GAAGGTGACC
AAGGGTGGCC
CCCTGCCCTT
CGCCTGGGAC
ATCCTGTCCC
CTCAGTTCAT
GTACGGCTCC
AAGGCCTACG
TGAAGCACCC
CGCCGACATC
CCCGACTACT
TGAAGCTGTC
CTTCCCCGAG
GGCTTCAAGT
GGGAGCGCGT
GATGAACTTC
GAGGACGGCG
GCGTGGTGAC
CGTGACCCAG
GACTCCTCAC
TGCAGGACGG
CGAGTTCATC
TACAAGGTGA
AGCTGCGCGG
CACCAACTTC
CCCTCCGACG
GCCCCGTAAT
GCAGAAGAAG
ACCATGGGCT
GGGAGGCCTC
CTCCGAGCGG
ATGTACCCCG
AGGACGGCGC
CCTGAAGGGC
GAGATCAAGC
AGAGGCTGAA
GCTGAAGGAC
GGCGGCCACT
ACGACGCTGA
GGTCAAGACC
ACCTACAAGG
CCAAGAAGCC
CGTGCAGCTG
CCCGGCGCCT
ACAACGTCAA
CATCAAGTTG
GACATCACCT
CCCACAACGA
GGACTACACC
ATCGTGGAAC
AGTACGAACG
CGCTGAGGGC
CGCCACTCCA
CCGGCGGCAT
GGACGAGCTG
TACAAGCTCG
AGTAGGGTAA
TCTAGATAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAATCATCGT
CCTTTCCTTG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCCTGA
GATCCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAC
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTAGTA
GTTCATGTCA
TCTTATTATT
CAGTATTTAT
AACTTGCAAA
GAAATGAATA
TCAGAGAGTG
AGAGGCCCGG
GTTAATTAAG
GAAAGGGCTA
GATCATTCTT
GAAGACGAAA
GGGCCTCGTG
ATACGCCTAT
TTTTATAGGT
TAATGTCATG
ATAATAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTGTTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TTCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAGAGCGCC
CAATACGCAA
ACCGCCTCTC
CCCGCGCGTT
GGCCGATTCA
TTAATGCAGC
AAGCTCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCGGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGCTTTTGC
AAAAAGCTCC
CCGTGGCACG
ACAGGTTTCC
CGACTGGAAA
GCGGGCAGTG
AGCGCAACGC
AATTAATGTG
AGTTAGCTCA
CTCATTAGGC
ACCCCAGGCT
TTACACTTTA
TGCTTCCGGC
TCGTATGTTG
TGTGGAATTG
TGAGCGGATA
ACAATTTCAC
ACAGGAAACA
GCTATGACAT
GATTACGAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGGAT
CAACTGGATA
ACTCAAGCTA
ACCAAAATCA
TCCCAAACTT
CCCACCCCAT
ACCCTATTAC
CACTGCCAAT
TACCTGTGGT
TTCATTTACT
CTAAACCTGT
GATTCCTCTG
AATTATTTTC
ATTTTAAAGA
AATTGTATTT
GTTAAATATG
TACTACAAAC
TTAGTAGTTG
GAAGGGCTAA
TTCACTCCCA
AAGAAGACAA
GATATCCTTG
ATCTGTGGAT
CTACCACACA
CAAGGCTACT
TCCCTGATTA
GCAGAACTAC
ACACCAGGGC
CAGGGGTCAG
ATATCCACTG
ACCTTTGGAT
GGTGCTACAA
GCTAGTACCA
GTTGAGCCAG
ATAAGGTAGA
AGAGGCCAAT
AAAGGAGAGA
ACACCAGCTT
GTTACACCCT
GTGAGCCTGC
ATGGGATGGA
TGACCCGGAG
AGAGAAGTGT
TAGAGTGGAG
GTTTGACAGC
CGCCTAGCAT
TTCATCACGT
GGCCCGAGAG
CTGCATCCGG
AGTACTTCAA
GAACTGCTGA
TATCGAGCTT
GCTACAAGGG
ACTTTCCGCT
GGGGACTTTC
CAGGGAGGCG
TGGCCTGGGC
GGGACTGGGG
AGTGGCGAGC
CCTCAGATCC
TGCATATAAG
CAGCTGCTTT
TTGCCTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTGGCGCC
CGAACAGGGA
CTTGAAAGCG
AAAGGGAAAC
CAGAGGAGCT
CTCTCGACGC
AGGACTCGGC
TTGCTGAAGC
GCGCACGGCA
AGAGGCGAGG
GGCGGCGACT
GGTGAGTACG
CCAAAAATTT
TGACTAGCGG
AGGCTAGAAG
GAGAGAGATG
GGTGCGAGAG
CGTCAGTATT
AAGCGGGGGA
GAATTAGATC
GCGATGGGAA
AAAATTCGGT
TAAGGCCAGG
GGGAAAGAAA
AAATATAAAT
TAAAACATAT
AGTATGGGCA
AGCAGGGAGC
TAGAACGATT
CGCAGTTAAT
CCTGGCCTGT
TAGAAACATC
AGAAGGCTGT
AGACAAATAC
TGGGACAGCT
ACAACCATCC
CTTCAGACAG
GATCAGAAGA
ACTTAGATCA
TTATATAATA
CAGTAGCAAC
CCTCTATTGT
GTGCATCAAA
GGATAGAGAT
AAAAGACACC
AAGGAAGCTT
TAGACAAGAT
AGAGGAAGAG
CAAAACAAAA
GTAAGACCAC
CGCACAGCAA
GCGGCCGGCC
GCTGATCTTC
AGACCTGGAG
GAGGAGATAT
GAGGGACAAT
TGGAGAAGTG
AATTATATAA
ATATAAAGTA
GTAAAAATTG
AACCATTAGG
AGTAGCACCC
ACCAAGGCAA
AGAGAAGAGT
GGTGCAGAGA
GAAAAAAGAG
CAGTGGGAAT
AGGAGCTTTG
TTCCTTGGGT
TCTTGGGAGC
AGCAGGAAGC
ACTATGGGCG
CAGCGTCAAT
GACGCTGACG
GTACAGGCCA
GACAATTATT
GTCTGGTATA
GTGCAGCAGC
AGAACAATTT
GCTGAGGGCT
ATTGAGGCGC
AACAGCATCT
GTTGCAACTC
ACAGTCTGGG
GCATCAAGCA
GCTCCAGGCA
AGAATCCTGG
CTGTGGAAAG
ATACCTAAAG
GATCAACAGC
TCCTGGGGAT
TTGGGGTTGC
TCTGGAAAAC
TCATTTGCAC
CACTGCTGTG
CCTTGGAATG
CTAGTTGGAG
TAATAAATCT
CTGGAACAGA
TTTGGAATCA
CACGACCTGG
ATGGAGTGGG
ACAGAGAAAT
TAACAATTAC
ACAAGCTTAA
TACACTCCTT
AATTGAAGAA
TCGCAAAACC
AGCAAGAAAA
GAATGAACAA
GAATTATTGG
AATTAGATAA
ATGGGCAAGT
TTGTGGAATT
GGTTTAACAT
AACAAATTGG
CTGTGGTATA
TAAAATTATT
CATAATGATA
GTAGGAGGCT
TGGTAGGTTT
AAGAATAGTT
TTTGCTGTAC
TTTCTATAGT
GAATAGAGTT
AGGCAGGGAT
ATTCACCATT
ATCGTTTCAG
ACCCACCTCC
CAACCCCGAG
GGGACCCGAC
AGGCCCGAAG
GAATAGAAGA
AGAAGGTGGA
GAGAGAGACA
GAGACAGATC
CATTCGATTA
GTGAACGGAT
CTCGACGGTA
TCGCCGAATT
GACAAATGGC
AGTATTCATC
CACAATTTTA
AAAGAAAAGG
GGGGATTGGG
GGGTACAGTG
CAGGGGAAAG
AATAGTAGAC
ATAATAGCAA
CAGACATACA
AACTAAAGAA
TTACAAAAAC
AAATTACAAA
AATTCAAAAT
TTTCGGGTTT
ATTACAGGGA
CAGCAGAGAT
CCAGTTTGGA
CTAGCGATGC
TAGCTGATGC
GGGCCCTAGT
TATTAATAGT
AATCAATTAC
GGGGTCATTA
GTTCATAGCC
CATATATGGA
GTTCCGCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAATGA
CGTATGTTCC
CATAGTAACG
CCAATAGGGA
CTTTCCATTG
ACGTCAATGG
GTGGAGTATT
TACGGTAAAC
TGCCCACTTG
GCAGTACATC
AAGTGTATCA
TATGCCAAGT
ACGCCCCCTA
TTGACGTCAA
TGACGGTAAA
TGGCCCGCCT
GGCATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG