名称: | pU6-MATN3(human)-sgRNA1-Cas9-Puro |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | U6,CBH |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pX459M |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTGGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
TTGATAGTTC
TCGTAGCGTA
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTTTTA
GCGCGTGCGC
CAATTCTGCA
GACAAATGGC
TCTAGAGTCG
ACACTAGTCT
CGAGATCGAT
AAGCTTCCTA
GAGGTACCCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
GTGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTCGAG
GTGAGCCCCA
CGTTCTGCTT
CACTCTCCCC
ATCTCCCCCC
CCTCCCCACC
CCCAATTTTG
TATTTATTTA
TTTTTTAATT
ATTTTGTGCA
GCGATGGGGG
CGGGGGGGGG
GGGGGGGCGC
GCGCCAGGCG
GGGCGGGGCG
GGGCGAGGGG
CGGGGCGGGG
CGAGGCGGAG
AGGTGCGGCG
GCAGCCAATC
AGAGCGGCGC
GCTCCGAAAG
TTTCCTTTTA
TGGCGAGGCG
GCGGCGGCGG
CGGCCCTATA
AAAAGCGAAG
CGCGCGGCGG
GCGGGAGTCG
CTGCGACGCT
GCCTTCGCCC
CGTGCCCCGC
TCCGCCGCCG
CCTCGCGCCG
CCCGCCCCGG
CTCTGACTGA
CCGCGTTACT
CCCACAGGTG
AGCGGGCGGG
ACGGCCCTTC
TCCTCCGGGC
TGTAATTAGC
TGAGCAAGAG
GTAAGGGTTT
AAGGGATGGT
TGGTTGGTGG
GGTATTAATG
TTTAATTACC
TGGAGCACCT
GCCTGAAATC
ACTTTTTTTC
AGGTTGGACC
GGTGCCACCA
TGGACTATAA
GGACCACGAC
GGAGACTACA
AGGATCATGA
TATTGATTAC
AAAGACGATG
ACGATAAGAT
GGCCCCAAAG
AAGAAGCGGA
AGGTCGGTAT
CCACGGAGTC
CCAGCAGCCG
ACAAGAAGTA
CAGCATCGGC
CTGGACATCG
GCACCAACTC
TGTGGGCTGG
GCCGTGATCA
CCGACGAGTA
CAAGGTGCCC
AGCAAGAAAT
TCAAGGTGCT
GGGCAACACC
GACCGGCACA
GCATCAAGAA
GAACCTGATC
GGAGCCCTGC
TGTTCGACAG
CGGCGAAACA
GCCGAGGCCA
CCCGGCTGAA
GAGAACCGCC
AGAAGAAGAT
ACACCAGACG
GAAGAACCGG
ATCTGCTATC
TGCAAGAGAT
CTTCAGCAAC
GAGATGGCCA
AGGTGGACGA
CAGCTTCTTC
CACAGACTGG
AAGAGTCCTT
CCTGGTGGAA
GAGGATAAGA
AGCACGAGCG
GCACCCCATC
TTCGGCAACA
TCGTGGACGA
GGTGGCCTAC
CACGAGAAGT
ACCCCACCAT
CTACCACCTG
AGAAAGAAAC
TGGTGGACAG
CACCGACAAG
GCCGACCTGC
GGCTGATCTA
TCTGGCCCTG
GCCCACATGA
TCAAGTTCCG
GGGCCACTTC
CTGATCGAGG
GCGACCTGAA
CCCCGACAAC
AGCGACGTGG
ACAAGCTGTT
CATCCAGCTG
GTGCAGACCT
ACAACCAGCT
GTTCGAGGAA
AACCCCATCA
ACGCCAGCGG
CGTGGACGCC
AAGGCCATCC
TGTCTGCCAG
ACTGAGCAAG
AGCAGACGGC
TGGAAAATCT
GATCGCCCAG
CTGCCCGGCG
AGAAGAAGAA
TGGCCTGTTC
GGAAACCTGA
TTGCCCTGAG
CCTGGGCCTG
ACCCCCAACT
TCAAGAGCAA
CTTCGACCTG
GCCGAGGATG
CCAAACTGCA
GCTGAGCAAG
GACACCTACG
ACGACGACCT
GGACAACCTG
CTGGCCCAGA
TCGGCGACCA
GTACGCCGAC
CTGTTTCTGG
CCGCCAAGAA
CCTGTCCGAC
GCCATCCTGC
TGAGCGACAT
CCTGAGAGTG
AACACCGAGA
TCACCAAGGC
CCCCCTGAGC
GCCTCTATGA
TCAAGAGATA
CGACGAGCAC
CACCAGGACC
TGACCCTGCT
GAAAGCTCTC
GTGCGGCAGC
AGCTGCCTGA
GAAGTACAAA
GAGATTTTCT
TCGACCAGAG
CAAGAACGGC
TACGCCGGCT
ACATTGACGG
CGGAGCCAGC
CAGGAAGAGT
TCTACAAGTT
CATCAAGCCC
ATCCTGGAAA
AGATGGACGG
CACCGAGGAA
CTGCTCGTGA
AGCTGAACAG
AGAGGACCTG
CTGCGGAAGC
AGCGGACCTT
CGACAACGGC
AGCATCCCCC
ACCAGATCCA
CCTGGGAGAG
CTGCACGCCA
TTCTGCGGCG
GCAGGAAGAT
TTTTACCCAT
TCCTGAAGGA
CAACCGGGAA
AAGATCGAGA
AGATCCTGAC
CTTCCGCATC
CCCTACTACG
TGGGCCCTCT
GGCCAGGGGA
AACAGCAGAT
TCGCCTGGAT
GACCAGAAAG
AGCGAGGAAA
CCATCACCCC
CTGGAACTTC
GAGGAAGTGG
TGGACAAGGG
CGCTTCCGCC
CAGAGCTTCA
TCGAGCGGAT
GACCAACTTC
GATAAGAACC
TGCCCAACGA
GAAGGTGCTG
CCCAAGCACA
GCCTGCTGTA
CGAGTACTTC
ACCGTGTATA
ACGAGCTGAC
CAAAGTGAAA
TACGTGACCG
AGGGAATGAG
AAAGCCCGCC
TTCCTGAGCG
GCGAGCAGAA
AAAGGCCATC
GTGGACCTGC
TGTTCAAGAC
CAACCGGAAA
GTGACCGTGA
AGCAGCTGAA
AGAGGACTAC
TTCAAGAAAA
TCGAGTGCTT
CGACTCCGTG
GAAATCTCCG
GCGTGGAAGA
TCGGTTCAAC
GCCTCCCTGG
GCACATACCA
CGATCTGCTG
AAAATTATCA
AGGACAAGGA
CTTCCTGGAC
AATGAGGAAA
ACGAGGACAT
TCTGGAAGAT
ATCGTGCTGA
CCCTGACACT
GTTTGAGGAC
AGAGAGATGA
TCGAGGAACG
GCTGAAAACC
TATGCCCACC
TGTTCGACGA
CAAAGTGATG
AAGCAGCTGA
AGCGGCGGAG
ATACACCGGC
TGGGGCAGGC
TGAGCCGGAA
GCTGATCAAC
GGCATCCGGG
ACAAGCAGTC
CGGCAAGACA
ATCCTGGATT
TCCTGAAGTC
CGACGGCTTC
GCCAACAGAA
ACTTCATGCA
GCTGATCCAC
GACGACAGCC
TGACCTTTAA
AGAGGACATC
CAGAAAGCCC
AGGTGTCCGG
CCAGGGCGAT
AGCCTGCACG
AGCACATTGC
CAATCTGGCC
GGCAGCCCCG
CCATTAAGAA
GGGCATCCTG
CAGACAGTGA
AGGTGGTGGA
CGAGCTCGTG
AAAGTGATGG
GCCGGCACAA
GCCCGAGAAC
ATCGTGATCG
AAATGGCCAG
AGAGAACCAG
ACCACCCAGA
AGGGACAGAA
GAACAGCCGC
GAGAGAATGA
AGCGGATCGA
AGAGGGCATC
AAAGAGCTGG
GCAGCCAGAT
CCTGAAAGAA
CACCCCGTGG
AAAACACCCA
GCTGCAGAAC
GAGAAGCTGT
ACCTGTACTA
CCTGCAGAAT
GGGCGGGATA
TGTACGTGGA
CCAGGAACTG
GACATCAACC
GGCTGTCCGA
CTACGATGTG
GACCATATCG
TGCCTCAGAG
CTTTCTGAAG
GACGACTCCA
TCGACAACAA
GGTGCTGACC
AGAAGCGACA
AGAACCGGGG
CAAGAGCGAC
AACGTGCCCT
CCGAAGAGGT
CGTGAAGAAG
ATGAAGAACT
ACTGGCGGCA
GCTGCTGAAC
GCCAAGCTGA
TTACCCAGAG
AAAGTTCGAC
AATCTGACCA
AGGCCGAGAG
AGGCGGCCTG
AGCGAACTGG
ATAAGGCCGG
CTTCATCAAG
AGACAGCTGG
TGGAAACCCG
GCAGATCACA
AAGCACGTGG
CACAGATCCT
GGACTCCCGG
ATGAACACTA
AGTACGACGA
GAATGACAAG
CTGATCCGGG
AAGTGAAAGT
GATCACCCTG
AAGTCCAAGC
TGGTGTCCGA
TTTCCGGAAG
GATTTCCAGT
TTTACAAAGT
GCGCGAGATC
AACAACTACC
ACCACGCCCA
CGACGCCTAC
CTGAACGCCG
TCGTGGGAAC
CGCCCTGATC
AAAAAGTACC
CTAAGCTGGA
AAGCGAGTTC
GTGTACGGCG
ACTACAAGGT
GTACGACGTG
CGGAAGATGA
TCGCCAAGAG
CGAGCAGGAA
ATCGGCAAGG
CTACCGCCAA
GTACTTCTTC
TACAGCAACA
TCATGAACTT
TTTCAAGACC
GAGATTACCC
TGGCCAACGG
CGAGATCCGG
AAGCGGCCTC
TGATCGAGAC
AAACGGCGAA
ACCGGGGAGA
TCGTGTGGGA
TAAGGGCCGG
GATTTTGCCA
CCGTGCGGAA
AGTGCTGAGC
ATGCCCCAAG
TGAATATCGT
GAAAAAGACC
GAGGTGCAGA
CAGGCGGCTT
CAGCAAAGAG
TCTATCCTGC
CCAAGAGGAA
CAGCGATAAG
CTGATCGCCA
GAAAGAAGGA
CTGGGACCCT
AAGAAGTACG
GCGGCTTCGA
CAGCCCCACC
GTGGCCTATT
CTGTGCTGGT
GGTGGCCAAA
GTGGAAAAGG
GCAAGTCCAA
GAAACTGAAG
AGTGTGAAAG
AGCTGCTGGG
GATCACCATC
ATGGAAAGAA
GCAGCTTCGA
GAAGAATCCC
ATCGACTTTC
TGGAAGCCAA
GGGCTACAAA
GAAGTGAAAA
AGGACCTGAT
CATCAAGCTG
CCTAAGTACT
CCCTGTTCGA
GCTGGAAAAC
GGCCGGAAGA
GAATGCTGGC
CTCTGCCGGC
GAACTGCAGA
AGGGAAACGA
ACTGGCCCTG
CCCTCCAAAT
ATGTGAACTT
CCTGTACCTG
GCCAGCCACT
ATGAGAAGCT
GAAGGGCTCC
CCCGAGGATA
ATGAGCAGAA
ACAGCTGTTT
GTGGAACAGC
ACAAGCACTA
CCTGGACGAG
ATCATCGAGC
AGATCAGCGA
GTTCTCCAAG
AGAGTGATCC
TGGCCGACGC
TAATCTGGAC
AAAGTGCTGT
CCGCCTACAA
CAAGCACCGG
GATAAGCCCA
TCAGAGAGCA
GGCCGAGAAT
ATCATCCACC
TGTTTACCCT
GACCAATCTG
GGAGCCCCTG
CCGCCTTCAA
GTACTTTGAC
ACCACCATCG
ACCGGAAGAG
GTACACCAGC
ACCAAAGAGG
TGCTGGACGC
CACCCTGATC
CACCAGAGCA
TCACCGGCCT
GTACGAGACA
CGGATCGACC
TGTCTCAGCT
GGGAGGCGAC
AAAAGGCCGG
CGGCCACGAA
AAAGGCCGGC
CAGGCAAAAA
AGAAAAAGGA
ATTCGGCAGT
GGAGAGGGCA
GAGGAAGTCT
GCTAACATGC
GGTGACGTCG
AGGAGAATCC
TGGCCCAATG
ACCGAGTACA
AGCCCACGGT
GCGCCTCGCC
ACCCGCGACG
ACGTCCCCAG
GGCCGTACGC
ACCCTCGCCG
CCGCGTTCGC
CGACTACCCC
GCCACGCGCC
ACACCGTCGA
TCCGGACCGC
CACATCGAGC
GGGTCACCGA
GCTGCAAGAA
CTCTTCCTCA
CGCGCGTCGG
GCTCGACATC
GGCAAGGTGT
GGGTCGCGGA
CGACGGCGCC
GCGGTGGCGG
TCTGGACCAC
GCCGGAGAGC
GTCGAAGCGG
GGGCGGTGTT
CGCCGAGATC
GGCCCGCGCA
TGGCCGAGTT
GAGCGGTTCC
CGGCTGGCCG
CGCAGCAACA
GATGGAAGGC
CTCCTGGCGC
CGCACCGGCC
CAAGGAGCCC
GCGTGGTTCC
TGGCCACCGT
CGGAGTCTCG
CCCGACCACC
AGGGCAAGGG
TCTGGGCAGC
GCCGTCGTGC
TCCCCGGAGT
GGAGGCGGCC
GAGCGCGCCG
GGGTGCCCGC
CTTCCTGGAG
ACCTCCGCGC
CCCGCAACCT
CCCCTTCTAC
GAGCGGCTCG
GCTTCACCGT
CACCGCCGAC
GTCGAGGTGC
CCGAAGGACC
GCGCACCTGG
TGCATGACCC
GCAAGCCCGG
TGCCTGAGAA
TTCTAACTAG
AGCTCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGAGAA
TAGCAGGCAT
GCTGGGGAGC
GGCCGCAGGA
ACCCCTAGTG
ATGGAGTTGG
CCACTCCCTC
TCTGCGCGCT
CGCTCGCTCA
CTGAGGCCGG
GCGACCAAAG
GTCGCCCGAC
GCCCGGGCTT
TGCCCGGGCG
GCCTCAGTGA
GCGAGCGAGC
GCGCAGCTGC
CTGCAGGGGC
GCCTGATGCG
GTATTTTCTC
CTTACGCATC
TGTGCGGTAT
TTCACACCGC
ATACGTCAAA
GCAACCATAG
TACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTTGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGGCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
TTAACAAAAT
ATTAACGTTT
ACAATTTTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGCCC
CGACACCCGC
CAACACCCGC
TGACGCGCCC
TGACGGGCTT
GTCTGCTCCC
GGCATCCGCT
TACAGACAAG
CTGTGACCGT
CTCCGGGAGC
TGCATGTGTC
AGAGGTTTTC
ACCGTCATCA
CCGAAACGCG
CGAGACGAAA
GGGCCTCGTG
ATACGCCTAT
TTTTATAGGT
TAATGTCATG
ATAATAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGA
AGCCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGT